CN103911412A - Preparation method of melon seed polypeptide - Google Patents
Preparation method of melon seed polypeptide Download PDFInfo
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- CN103911412A CN103911412A CN201410098345.5A CN201410098345A CN103911412A CN 103911412 A CN103911412 A CN 103911412A CN 201410098345 A CN201410098345 A CN 201410098345A CN 103911412 A CN103911412 A CN 103911412A
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Abstract
The invention provides a preparation method of a melon seed polypeptide. The method includes: putting 45-58 parts of melon seed pulp and 700-800 parts of distilled water into a water-bath container, adjusting the pH to 9 by utilization of a NaOH solution having a concentration of 1 mol/L, maintaining in the water bath having a temperature of 50 DEG C for 68-73 min, adjusting the pH to 4.5 by utilization of hydrochloric acid having a concentration of 1 mol/L, centrifuging for 20 min at a speed of 4000 rpm to obtain protein, processing the protein by a vacuum freeze drier to obtain protein powder, adding a protein solution into an enzymolysis tank, heating the protein solution to 50 DEG C, adding 9-130 U/g of cellulase and 25-35 U/g of papain, adjusting the pH to 7.0 by utilization of the NaOH solution having a concentration of 1 mol/L or the hydrochloric acid having a concentration of 1 mol/L, putting the mixture into the enzymolysis tank into an ultrasonic wave apparatus, performing enzymolysis under assistant for 2.8-3.2 h with the pH range being plus or minus 0.1, putting the mixture into a water bath having a temperature of 85 DEG C after the enzymolysis is finished, maintaining for 18-23 min, cooling to room temperature, performing enzyme deactivation, centrifuging for 18-22 min at a speed of 4000 rpm, collecting the liquid supernatant, and preparing the liquid supernatant into a finished product of the melon seed polypeptide by a vacuum freeze drier.
Description
Technical field
The present invention relates to nutritive health-care product, the sub-dregs of rice of a kind of watermelon of selecting extract protein and make polypeptide products through protease hydrolysis, and nutritive value is high.
Background technology
Kind of watermelon is nuts food, and nutritive value is very high, and it is not only a kind of high-protein food, and the trace elements such as the iron that is also rich in, zinc, potassium, magnesium have the generation of the diseases such as prevention anaemia.Squeezed domestic feed or the fertilizer mostly done of the sub-dregs of rice of a kind of watermelon after oil, cause utilize unreasonable.In the sub-dregs of rice of a kind of watermelon, contain the nutritive ingredients such as rich in protein, fat, Mierocrystalline cellulose, minerals and vitamins, large quantity research shows, select suitable proteolytic enzyme, control certain degree of hydrolysis, the sub-protein of a hydrolysis kind of watermelon can obtain large number of biological active polypeptide, these biologically active polypeptidess are easily digested, and have anti-oxidant, hypotensive, get rid of illness, diseases prevention, promoting digestion absorb and strengthen the physiological actions such as body immunity.
The sub-polypeptide of a kind of watermelon of the present invention, is that sub-the kind of watermelon dregs of rice are extracted after protein, utilizes double-enzyme method to prepare the sub-polypeptide of a kind of watermelon by ultrasound-assisted enzymolysis, becomes functional edible fine work, has wide market sale prospect.
Summary of the invention
The object of the invention is to: the sub-polypeptide of a kind of watermelon making, have anti-oxidant, hypotensive, get rid of illness, diseases prevention, promoting digestion absorb and strengthen the different physiological actions such as body immunity, is deeply subject to human consumer's favor.
The object of the present invention is achieved like this: the preparation method of the sub-polypeptide of an a kind of kind of watermelon, implements step by step;
Raw material: the sub-dregs of rice 45-58 of kind of watermelon part, distilled water 700-800 part, cellulase 9-130U/g, papoid 25-35U/g composition;
The preparation of step 1 protein powder:
By sub-the kind of watermelon dregs of rice, distilled water is placed in water bath containers, with the NaOH tune pH to 9 of 1mol/L, in the water-bath of 50 ℃, maintain 68-73min, with the HCl tune pH to 4.5 of 1mol/L, under 4000rpm condition, centrifugal 20min obtains albumen, disposes through vacuum freeze drier, makes protein powder;
The preparation of the sub-polypeptide of step 2 kind of watermelon:
Get protein powder and distilled water is made into protein liquid, its powder with water than being 1:50;
Protein liquid is placed in enzymatic vessel and is heated to 50 ℃, add cellulase, papoid, with the NaOH of 1mol/L or the HCl of 1mol/L tune pH to 7.0, again mixture in enzymatic vessel is placed in to ultrasonic drilling machine assistance enzymolysis, its ultrasonic time 30min, ultrasonic power is 60%, and ultrasonic temperature is 40 ℃; Enzymolysis time 2.8-3.2h, pH value mobility scale ± 0.1, enzymolysis finishes, be placed in again 85 ℃ of water-baths and maintain 18-23min, be cooled to the room temperature enzyme that goes out, under 4000rpm condition, centrifugal 18-22min, collects supernatant liquor, makes the sub-polypeptide finished product of a kind of watermelon through vacuum freeze drier.
Trophism of the present invention: the sub-polypeptide of a kind of watermelon providing is biologically active peptides, determine in the sub-polypeptide of a kind of watermelon and contain crude protein 91.35% according to GB/T22492-2008, peptide content 80.26%, ash content (in butt)/%6.5, moisture/%7.0, crude fat (butt)/%1.0, meet primary standard, in addition, the sub-polypeptide of a kind of watermelon has the effects such as anti-oxidant, hypotensive, shows technical progress.
Embodiment
Below in conjunction with embodiment, invention is described further.
Embodiment
The sub-polypeptide technical process of a kind of watermelon adopting:
The sub-dregs of rice → the leach protein of kind of watermelon matter → enzymolysis (ultrasonic wave is auxiliary) the sub-polypeptide finished product of → enzyme that goes out → centrifugal → vacuum lyophilization → kind of watermelon.
1) sample pretreatment: sub-the kind of watermelon dregs of rice are pulverized with high speed disintegrator, crossed 40 mesh sieves for subsequent use;
2) leach protein matter: get 750 parts of 50 parts of the sub-dregs of rice of a kind of watermelon, distilled water and be placed in water bath containers, with 1mol/LNaOH tune pH to 9, at temperature 50 C, water-bath maintains 70min, with the HCl tune pH to 4.5 of 1mol/L, under the condition of rotating speed 4000rpm, centrifugal 20min, obtain protein isolate, by vacuum lyophilization, obtain protein powder.
3) enzymolysis:
Get protein powder and distilled water 1:50, be made into protein liquid;
The above-mentioned protein liquid being made into is positioned in enzymatic vessel, be heated to 50 ℃, add cellulase 11 0U/g, papoid 30U/g, be adjusted to pH7.0 with the NaOH of 1mol/L or the HCl of 1mol/L, add ultrasound-assisted enzymolysis, ultrasonic time is 30min, and ultrasonic power is 60%, and ultrasonic temperature is 40 ℃; Wherein enzyme digestion reaction 3h(pH value mobility scale ± 0.1) after, in 85 ℃ of water-baths, maintain 20min, enzyme is carried out to inactivation treatment, be cooled to room temperature, under 4000rpm condition, centrifugal 20min, collects supernatant liquor.
4) vacuum lyophilization: the supernatant liquor of collection is made to the sub-polypeptide finished product of a kind of watermelon by vacuum lyophilization.
It is Q/XZZ-2013 " the sub-polypeptide of a kind of watermelon " that this product meets Changji Hui Autonomous Prefecture, Xinjiang Minister of the General Administration for Quality Supervision formulation establishment company standard.The sub-polypeptide of a kind of watermelon of producing has identified by medicine inspecting institute of autonomous region and Department of Public Health of autonomous region verifies this product, and obtains " food of special nutrients " certificate.
The index of this product detects:
Oranoleptic indicator: faint yellow, there is the distinctive flavour of this product and smell.
Physical and chemical index: crude protein 91.35%; Peptide content 80.26%; VE=0.25mg/100gl, VA=0.13mg/100g, VB1=0.09mg/100g, VC=268mg/100g ash content (in butt)/%≤6.5, moisture/%≤7.0, crude fat (butt)/%≤1.0.
Microbiological indicator: total number of bacterial colony < 1 (cfu/ml); Coliform < 3 (MPN/100ml); Pathogenic bacterium do not detect.
Kind of watermelon Zi Poshi our company that present method is selected produces the byproduct after kind of watermelon seed oil; Cellulase is purchased from Nanning Chong Bo biotechnology company limited; Papoid is purchased from Nanning Chong Bo biotechnology company limited; BYK type vacuum freeze drier is purchased from Beijing universal love health laboratory apparatus company limited.
Claims (2)
1. a preparation method for the sub-polypeptide of a kind of watermelon, is characterized in that: implement step by step;
Raw material: the sub-dregs of rice 45-58 of kind of watermelon part, distilled water 700-800 part, cellulase 9-130U/g, papoid 25-35U/g composition;
The preparation of step 1 protein powder:
By sub-the kind of watermelon dregs of rice, distilled water is placed in water bath containers, with the NaOH tune pH to 9 of 1mol/L, in the water-bath of 50 ℃, maintain 68-73min, with the HCl tune pH to 4.5 of 1 mol/L, under 4000rpm condition, centrifugal 20min obtains albumen, disposes through vacuum freeze drier, makes protein powder;
The preparation of the sub-polypeptide of step 2 kind of watermelon:
Protein liquid preparation: get protein powder and distilled water, its solid-liquid ratio is that 1:50 makes;
Protein liquid is placed in enzymatic vessel and is heated to 50 ℃, add cellulase, papoid, with the NaOH of 1mol/L or the HCl of 1 mol/L, adjust pH to 7.0, again mixture in enzymatic vessel is placed in to ultrasonic drilling machine assistance enzymolysis, its ultrasonic time 30min, ultrasonic power is 60%, ultrasonic temperature is 40 ℃; Enzymolysis time 2.8-3.2h, pH value scope ± 0.1, enzymolysis finishes, then is placed in 85 ℃ of water-baths, maintains 18-23min, is cooled to the room temperature enzyme that goes out, and under 4000rpm condition, centrifugal 18-22min, collects supernatant liquor, makes the sub-polypeptide finished product of a kind of watermelon through vacuum freeze drier.
2. according to the preparation method described in letter of authorization 1, it is characterized in that: the sub-dregs of rice of a kind of watermelon are that our company produces the byproduct after kind of watermelon seed oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410098345.5A CN103911412A (en) | 2014-03-14 | 2014-03-14 | Preparation method of melon seed polypeptide |
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| CN201410098345.5A CN103911412A (en) | 2014-03-14 | 2014-03-14 | Preparation method of melon seed polypeptide |
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| CN103911412A true CN103911412A (en) | 2014-07-09 |
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| CN201410098345.5A Pending CN103911412A (en) | 2014-03-14 | 2014-03-14 | Preparation method of melon seed polypeptide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531814A (en) * | 2014-12-05 | 2015-04-22 | 中南林业科技大学 | Preparation method of oil-tea meal enzymatically-modified protein |
| CN107557422A (en) * | 2017-10-24 | 2018-01-09 | 西北民族大学 | A kind of cottonseed protein polypeptide with high ACE inhibitory activity and preparation method thereof |
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2014
- 2014-03-14 CN CN201410098345.5A patent/CN103911412A/en active Pending
Patent Citations (6)
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| CN1462594A (en) * | 2002-09-30 | 2003-12-24 | 马永东 | Method for preparing powder of black melon seed from seed melon and melon seed beverage |
| CN1632131A (en) * | 2004-11-02 | 2005-06-29 | 通化腾龙保健品有限公司 | Process for biological enzyme extraction of grape seed polypeptide protein |
| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
| CN102653780A (en) * | 2011-03-03 | 2012-09-05 | 徐金军 | Method for preparing low molecular peptide through proteolysis of pumpkin seeds and dregs |
| CN102197856A (en) * | 2011-06-22 | 2011-09-28 | 山西大学 | Preparation method of pumpkin seed antioxidant peptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531814A (en) * | 2014-12-05 | 2015-04-22 | 中南林业科技大学 | Preparation method of oil-tea meal enzymatically-modified protein |
| CN104531814B (en) * | 2014-12-05 | 2017-11-14 | 中南林业科技大学 | A kind of preparation method of Extracted From Oil-tea-cake enzyme modification albumen |
| CN107557422A (en) * | 2017-10-24 | 2018-01-09 | 西北民族大学 | A kind of cottonseed protein polypeptide with high ACE inhibitory activity and preparation method thereof |
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