CN103908474A - Method for producing and processing rabbit brain powder - Google Patents
Method for producing and processing rabbit brain powder Download PDFInfo
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- CN103908474A CN103908474A CN201410164727.3A CN201410164727A CN103908474A CN 103908474 A CN103908474 A CN 103908474A CN 201410164727 A CN201410164727 A CN 201410164727A CN 103908474 A CN103908474 A CN 103908474A
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- acetone
- meninges
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- seu oryctolagi
- leporis seu
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Abstract
The invention relates to a method for producing and processing rabbit brain powder. The method for producing and processing rabbit brain powder includes the following steps: (1) soaking fresh rabbit brains in low-temperature acetone or physiological saline, and removing blood vessels and meninges; (2) putting the rabbit brains with blood vessels and meninges removed in an extruding device soaked in acetone, and extruding the rabbit brains from the extruding device into a grinding machine; (3) grinding the rabbit brains by using the grinding machine in a slow grinding manner; (4) after the grinding operation, starting a filter screen at the bottom of the grinding machine to further filter out the blood vessels and meninge impurities, adding the acetone, slightly shaking for multiple times, centrifuging, then removing the acetone solvent; drying, packaging and storing at 4 DEG C. The method for producing and processing rabbit brain powder provided by the invention is used for further removing more than 70% of the residual blood vessels and meninge impurities by extruding and filtering; 20% of acetone is added before the grinding type crushing and centrifuging steps, so as to reduce the colloid state of the solution, thereby reducing the difficulty of subsequent centrifuging and drying and better keep the activity of the product.
Description
Technical field
The present invention relates to the production and processing method of rabbit brain powder.
Background technology
Tissue factor (tissue factor) is that film relies on glycoprotein in conjunction with fat, and it can form complex with factor VII, significantly improves the activation capacity of factor VII to F10 and IX.Tissue factor reacts to bring into play anastalsis by activating coagulation cascade, just contains abundant tissue factor, therefore rabbit brain powder is widely used in the Clinical Laboratory of medical science in the rabbit brain powder of acetone extraction.
At present rabbit brain powder production and processing mostly adopts to remove and pulverizes after blood vessel and meninges or the method for homogenate.The patent No. is the processing method that 201110070215.7 Chinese patent discloses a kind of suitability for industrialized production rabbit brain powder, and the method adds cold acetone homogenate to mix then centrifugal drying after will fresh Medulla Leporis seu Oryctolagi removing blood vessel and meninges.Because cerebral tissue is combined closely with blood vessel and meninges, adopt the method not remove completely, follow-up direct homogenate, pulverizing and dryly will cause pulverizing cost increasing, product medium vessels and meninges impurity are many, and homogenate grinding mode causes Medulla Leporis seu Oryctolagi and acetone to form colloidal solution, separation process difficulty.
Summary of the invention
The object of the invention is to solve the problem that prior art exists, a kind of rabbit brain powder production and processing method is provided, reduce finished product medium vessels and meninges impurity content, avoid forming colloidal solution, reduce separating difficulty.
To achieve these goals, the technical solution used in the present invention is: (1), fresh Medulla Leporis seu Oryctolagi is placed in to 10 DEG C of following acetone or normal saline soaks, remove blood vessel and meninges;
(2) Medulla Leporis seu Oryctolagi of, removing blood vessel and meninges is put into the pressurizing unit that acetone soaks, the volume of controlling acetone is 1.5～3 times of Medulla Leporis seu Oryctolagi weight, Medulla Leporis seu Oryctolagi in pressurizing unit is clamp-oned to grinder, reject the remaining blood vessel of part and meninges in Medulla Leporis seu Oryctolagi by the hour hand shape tuck of pressurizing unit bottom;
(3), grinder grinds 10min left and right in the mode of grinding at a slow speed by Medulla Leporis seu Oryctolagi;
(4), start the filter screen of grinder bottom after grinding, further filtering blood vessel and meninges impurity, then add the acetone of above-mentioned acetone volume 20%, gently shake several carry out after lower centrifugal, centrifugal rear removal acetone solvent, dry, subpackage, 4 DEG C of stored refrigerated.
The speed of grinding is at a slow speed 1～2 second/time.
Rabbit brain powder production and processing method of the present invention is further removed remaining more than 70% blood vessel and meninges impurity by pushing and filter two steps; By abrading type pulverize and centrifugal before add again 20% acetone, reduce the colloidal state of solution, in reducing follow-up centrifugal and dry difficulty, more keep the activity of product.
Detailed description of the invention
1 one kinds of rabbit brain powder production and processing methods of embodiment, comprise the following steps:
1, the acetone that fresh Medulla Leporis seu Oryctolagi is placed in to zero degree soaked about 10 minutes, then removed blood vessel and the meninges in Medulla Leporis seu Oryctolagi with tweezers;
2, the Medulla Leporis seu Oryctolagi of removing blood vessel and meninges is put into the pressurizing unit that acetone soaks, the volume of controlling acetone is 2 times of Medulla Leporis seu Oryctolagi weight, and the hour hand shape tuck of pressurizing unit bottom is rejected the remaining blood vessel of part and meninges in Medulla Leporis seu Oryctolagi;
3, by the extrusion piston in the middle of pressurizing unit, Medulla Leporis seu Oryctolagi is clamp-oned to grinder, grinder grinds Medulla Leporis seu Oryctolagi 10 minutes with the speed of 1 second/time;
4, start the filter screen of grinder bottom after grinding, further filtering blood vessel and meninges impurity, then add 20% acetone, gently shake several carry out after lower centrifugal, 2000rpm ∕ min, centrifugal 3 minutes, centrifugal rear removal acetone solvent, vacuum drying; Subpackage, 4 DEG C of stored refrigerated.
Claims (1)
1. a rabbit brain powder production and processing method, comprises the following steps: (1), fresh Medulla Leporis seu Oryctolagi is placed in to 10 DEG C of following acetone or normal saline soaks, remove blood vessel and meninges;
(2) Medulla Leporis seu Oryctolagi of, removing blood vessel and meninges is put into the pressurizing unit that acetone soaks, the volume of controlling acetone is 1.5～3 times of Medulla Leporis seu Oryctolagi weight, Medulla Leporis seu Oryctolagi in pressurizing unit is clamp-oned to grinder, reject the remaining blood vessel of part and meninges in Medulla Leporis seu Oryctolagi by the hour hand shape tuck of pressurizing unit bottom;
(3), grinder grinds 10min left and right in the mode of grinding at a slow speed by Medulla Leporis seu Oryctolagi;
(4), start the filter screen of grinder bottom after grinding, further filtering blood vessel and meninges impurity, then add the acetone of above-mentioned acetone volume 20%, gently shake several carry out after lower centrifugal, centrifugal rear removal acetone solvent, dry, subpackage, 4 DEG C of stored refrigerated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410164727.3A CN103908474B (en) | 2014-04-23 | 2014-04-23 | A kind of rabbit brain powder production and processing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410164727.3A CN103908474B (en) | 2014-04-23 | 2014-04-23 | A kind of rabbit brain powder production and processing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103908474A true CN103908474A (en) | 2014-07-09 |
| CN103908474B CN103908474B (en) | 2017-08-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410164727.3A Active CN103908474B (en) | 2014-04-23 | 2014-04-23 | A kind of rabbit brain powder production and processing method |
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| Country | Link |
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| CN (1) | CN103908474B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180958A (en) * | 2011-03-23 | 2011-09-14 | 宁波大学 | Processing method for industrially producing rabbit brain powder |
| CN102302768A (en) * | 2011-09-04 | 2012-01-04 | 潘首德 | Method for preparing brain protein hydrolysate oral liquid |
-
2014
- 2014-04-23 CN CN201410164727.3A patent/CN103908474B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180958A (en) * | 2011-03-23 | 2011-09-14 | 宁波大学 | Processing method for industrially producing rabbit brain powder |
| CN102302768A (en) * | 2011-09-04 | 2012-01-04 | 潘首德 | Method for preparing brain protein hydrolysate oral liquid |
Non-Patent Citations (2)
| Title |
|---|
| 梁彬: "关于自制兔脑凝血质代替进口兔脑凝血质的探索", 《卫生职业教育》 * |
| 郑智民: "《啮齿动物学》", 31 August 2012 * |
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| Publication number | Publication date |
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| CN103908474B (en) | 2017-08-11 |
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Effective date of registration: 20211011 Address after: 266406 zhangjialou Town, Huangdao District, Qingdao City, Shandong Province Patentee after: QINGDAO KONDE BIOTECHNOLOGY CO.,LTD. Address before: 266500 No. 1, Hainan Road, Huangdao District, Qingdao, Shandong Patentee before: QINGDAO KANGDA FOREIGN TRADE GROUP Co.,Ltd. |
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Effective date of registration: 20220819 Address after: Kangda Industrial Park, No. 317 Binhai Avenue, Huangdao District, Qingdao City, Shandong Province, 266000 Patentee after: Qingdao Kangda Aibo Biotechnology Co.,Ltd. Address before: 266406 resident of zhangjialou Town, Huangdao District, Qingdao City, Shandong Province Patentee before: QINGDAO KONDE BIOTECHNOLOGY CO.,LTD. |
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