CN103908474B - A kind of rabbit brain powder production and processing method - Google Patents
A kind of rabbit brain powder production and processing method Download PDFInfo
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- CN103908474B CN103908474B CN201410164727.3A CN201410164727A CN103908474B CN 103908474 B CN103908474 B CN 103908474B CN 201410164727 A CN201410164727 A CN 201410164727A CN 103908474 B CN103908474 B CN 103908474B
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- rabbit brain
- acetone
- meninx
- blood vessel
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Abstract
The present invention relates to the production and processing method of rabbit brain powder.A kind of rabbit brain powder production and processing method, comprises the following steps：（1）, new fresh rabbit brain is placed in the acetone of low temperature or physiological saline and soaked, remove blood vessel and meninx；（2）, the rabbit brain that removes blood vessel and meninx be put into the pressurizing unit of acetone soak, the rabbit brain in pressurizing unit is squeezed into grinder；（3）, grinder grinds rabbit brain in the way of grinding at a slow speed；（4）, start the filter screen of grinding motor spindle after grinding, further filter out blood vessel and meninx impurity, add acetone, gently shake it is several it is lower after centrifuged, acetone solvent is removed after centrifugation, is dried, is dispensed, 4 DEG C are stored refrigerated.The rabbit brain powder production and processing method of the present invention is by extruding and filtering blood vessel and meninx impurity that two steps further remove remaining more than 70%；20% acetone is added before crushing and centrifuge by abrading type, the colloidal state of solution is reduced, the activity of product is more kept while reducing follow-up centrifugation and drying difficulty.
Description
Technical field
The present invention relates to the production and processing method of rabbit brain powder.
Background technology
Tissue factor (tissue factor) is that film combination fat relies on glycoprotein, and it can form compound with factor VII
Thing, significantly improves factor VII to F10 and Ⅸ activation capacity.Tissue factor be by activate coagulation cascade reaction come
Anastalsis is played, just containing abundant tissue factor in the rabbit brain powder of acetone extraction, therefore rabbit brain powder is widely used in doctor
In clinical examination.
Current rabbit brain powder produces and processes the method crushed or be homogenized after blood vessel and meninx using removing mostly.Patent No.
201110070215.7 Chinese patent discloses a kind of processing method of industrialized production rabbit brain powder, and this method is by new fresh rabbit brain
Remove addition cold acetone homogenate mixing after blood vessel and meninx and be then centrifuged for drying.Because brain tissue is combined tightly with blood vessel and meninx
It is close, it can not possibly be removed completely using the method, follow-up directly homogenate, crushing and drying will cause to crush in added cost, product
Blood vessel and meninx impurity are more, and homogenate grinding mode causes rabbit brain and acetone formation colloidal solution, and separation process is difficult.
The content of the invention
There is provided a kind of rabbit brain powder production and processing method, reduction for the problem of existing present invention aim to address prior art
Finished product medium vessels and meninx impurity content, it is to avoid form colloidal solution, reduce separating difficulty.
To achieve these goals, the technical solution adopted by the present invention is：（1）, new fresh rabbit brain is placed in less than 10 DEG C
Soaked in acetone or physiological saline, remove blood vessel and meninx；
（2）, the rabbit brain that removes blood vessel and meninx be put into the pressurizing unit of acetone soak, the volume of control acetone is rabbit brain weight
1.5~3 times of amount, grinder is squeezed into by the rabbit brain in pressurizing unit, and rabbit is rejected by the hour hand shape tuck of pressurizing unit bottom
The remaining blood vessel in part and meninx in brain；
（3）, grinder rabbit brain is ground into 10min or so in the way of grinding at a slow speed；
（4）, start the filter screen of grinding motor spindle after grinding, further filter out blood vessel and meninx impurity, add above-mentioned third
Ketoboidies product 20% acetone, gently shake it is several it is lower after centrifuged, acetone solvent is removed after centrifugation, dry, packing, 4 DEG C stored refrigerated.
The speed ground at a slow speed is 1~2 second/time.
The present invention rabbit brain powder production and processing method by extrude and filtering two steps further remove remaining 70% with
On blood vessel and meninx impurity；20% acetone is added before crushing and centrifuge by abrading type, the colloidal state of solution is reduced,
The activity of product is more kept while the follow-up centrifugation of reduction and dry difficulty.
Embodiment
A kind of rabbit brain powder production and processing method of embodiment 1, comprises the following steps：
1st, new fresh rabbit brain is placed in the acetone of zero degree and soaked 10 minutes or so, then remove the blood vessel in rabbit brain with tweezers
And meninx；
The 2nd, the rabbit brain for removing blood vessel and meninx is put into the pressurizing unit of acetone soak, the volume of control acetone is rabbit brain weight
2 times of amount, the hour hand shape tuck of pressurizing unit bottom rejects the remaining blood vessel in part and meninx in rabbit brain；
3rd, rabbit brain is squeezed into by grinder by the extrusion piston in the middle of pressurizing unit, grinder is with 1 second/time of speed by rabbit
Brain is ground 10 minutes；
4th, grind after start grinding motor spindle filter screen, further filter out blood vessel and meninx impurity, add 20% third
Ketone, gently shake it is several it is lower after centrifuged, 2000rpm ∕ min, centrifuge 3 minutes, acetone solvent is removed after centrifugation, vacuum drying；Packing,
4 DEG C stored refrigerated.
Claims (1)
1. a kind of rabbit brain powder production and processing method, comprises the following steps：(1), by new fresh rabbit brain be placed in less than 10 DEG C acetone or
Soaked in physiological saline, remove blood vessel and meninx；
(2), the rabbit brain of removal blood vessel and meninx is put into the pressurizing unit of acetone soak, and the volume of control acetone is rabbit brain weight
1.5~3 times, the rabbit brain in pressurizing unit is squeezed into grinder, rejected by the hour hand shape tuck of pressurizing unit bottom in rabbit brain
The blood vessel and meninx of some residual；
(3), rabbit brain is ground 10min or so by grinder in the way of grinding at a slow speed, and the speed ground at a slow speed is 1~2 second/time；
(4) start the filter screen of grinding motor spindle after, grinding, further filter out blood vessel and meninx impurity, add above-mentioned acetone body
Product 20% acetone, gently shake it is several it is lower after centrifuged, acetone solvent is removed after centrifugation, dry, dispense, 4 DEG C stored refrigerated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410164727.3A CN103908474B (en) | 2014-04-23 | 2014-04-23 | A kind of rabbit brain powder production and processing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410164727.3A CN103908474B (en) | 2014-04-23 | 2014-04-23 | A kind of rabbit brain powder production and processing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103908474A CN103908474A (en) | 2014-07-09 |
| CN103908474B true CN103908474B (en) | 2017-08-11 |
Family
ID=51034678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410164727.3A Active CN103908474B (en) | 2014-04-23 | 2014-04-23 | A kind of rabbit brain powder production and processing method |
Country Status (1)
| Country | Link |
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| CN (1) | CN103908474B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180958A (en) * | 2011-03-23 | 2011-09-14 | 宁波大学 | Processing method for industrially producing rabbit brain powder |
| CN102302768A (en) * | 2011-09-04 | 2012-01-04 | 潘首德 | Method for preparing brain protein hydrolysate oral liquid |
-
2014
- 2014-04-23 CN CN201410164727.3A patent/CN103908474B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180958A (en) * | 2011-03-23 | 2011-09-14 | 宁波大学 | Processing method for industrially producing rabbit brain powder |
| CN102302768A (en) * | 2011-09-04 | 2012-01-04 | 潘首德 | Method for preparing brain protein hydrolysate oral liquid |
Non-Patent Citations (1)
| Title |
|---|
| 关于自制兔脑凝血质代替进口兔脑凝血质的探索;梁彬;《卫生职业教育》;20010330;第19卷(第3期);第36页。 * |
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| Publication number | Publication date |
|---|---|
| CN103908474A (en) | 2014-07-09 |
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Effective date of registration: 20211011 Address after: 266406 zhangjialou Town, Huangdao District, Qingdao City, Shandong Province Patentee after: QINGDAO KONDE BIOTECHNOLOGY CO.,LTD. Address before: 266500 No. 1, Hainan Road, Huangdao District, Qingdao, Shandong Patentee before: QINGDAO KANGDA FOREIGN TRADE GROUP Co.,Ltd. |
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Effective date of registration: 20220819 Address after: Kangda Industrial Park, No. 317 Binhai Avenue, Huangdao District, Qingdao City, Shandong Province, 266000 Patentee after: Qingdao Kangda Aibo Biotechnology Co.,Ltd. Address before: 266406 resident of zhangjialou Town, Huangdao District, Qingdao City, Shandong Province Patentee before: QINGDAO KONDE BIOTECHNOLOGY CO.,LTD. |