CN103898161A - 在禽类中进行转基因表达 - Google Patents
在禽类中进行转基因表达 Download PDFInfo
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- CN103898161A CN103898161A CN201410006618.9A CN201410006618A CN103898161A CN 103898161 A CN103898161 A CN 103898161A CN 201410006618 A CN201410006618 A CN 201410006618A CN 103898161 A CN103898161 A CN 103898161A
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| CN105331637A (zh) * | 2015-12-10 | 2016-02-17 | 浙江大学 | 一种鸡卵清蛋白启动子慢病毒载体、构建方法及用途 |
| CN106048043A (zh) * | 2016-07-11 | 2016-10-26 | 山东农业大学 | 鸡pthlh基因5′调控区两个突变位点的分子标记方法及其在鸡育种中的应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101652475B (zh) * | 2007-01-26 | 2014-03-19 | 辛那杰瓦生物制药股份有限公司 | 在禽类中进行转基因表达 |
| US9150847B2 (en) * | 2011-09-21 | 2015-10-06 | Sangamo Biosciences, Inc. | Methods and compositions for regulation of transgene expression |
| US9999208B2 (en) * | 2013-11-15 | 2018-06-19 | University Of Maryland Baltimore County | Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos |
| RU2741645C2 (ru) | 2015-05-19 | 2021-01-28 | Юниверсити Оф Мэрилэнд Балтимор Каунти | Способы доставки агента в икринки рыб |
Citations (4)
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|---|---|---|---|---|
| WO2000011151A2 (en) * | 1998-08-25 | 2000-03-02 | University Of Georgia Research Foundation, Inc. | Direct oviduct transgenesis |
| WO2003022040A2 (en) * | 2001-09-13 | 2003-03-20 | California Institute Of Technology | Method for producing transgenic animals |
| WO2004022722A2 (en) * | 2002-09-06 | 2004-03-18 | Massachusetts Institute Of Technology | Lentiviral vectors, related reagents, and methods of use thereof |
| CN101652475B (zh) * | 2007-01-26 | 2014-03-19 | 辛那杰瓦生物制药股份有限公司 | 在禽类中进行转基因表达 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6362504B1 (en) * | 1995-11-22 | 2002-03-26 | Philips Electronics North America Corporation | Contoured nonvolatile memory cell |
| US6825396B2 (en) * | 1996-06-12 | 2004-11-30 | Board Of Trustees Operating Michigan State University | Methods for tissue specific synthesis of protein in eggs of transgenic hens |
| JP2006262875A (ja) * | 2005-03-25 | 2006-10-05 | Hiroshima Univ | 鳥類の卵管内でタンパク質を発現させるための遺伝子構築物、およびこれを用いたタンパク質の生産方法 |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000011151A2 (en) * | 1998-08-25 | 2000-03-02 | University Of Georgia Research Foundation, Inc. | Direct oviduct transgenesis |
| WO2003022040A2 (en) * | 2001-09-13 | 2003-03-20 | California Institute Of Technology | Method for producing transgenic animals |
| WO2004022722A2 (en) * | 2002-09-06 | 2004-03-18 | Massachusetts Institute Of Technology | Lentiviral vectors, related reagents, and methods of use thereof |
| CN101652475B (zh) * | 2007-01-26 | 2014-03-19 | 辛那杰瓦生物制药股份有限公司 | 在禽类中进行转基因表达 |
Non-Patent Citations (1)
| Title |
|---|
| 汪维 等: "自我失活型逆转录病毒载体在衰老基因转录调控中的应用", 《物理化学与生物物理进展》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105331637A (zh) * | 2015-12-10 | 2016-02-17 | 浙江大学 | 一种鸡卵清蛋白启动子慢病毒载体、构建方法及用途 |
| CN106048043A (zh) * | 2016-07-11 | 2016-10-26 | 山东农业大学 | 鸡pthlh基因5′调控区两个突变位点的分子标记方法及其在鸡育种中的应用 |
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| JP5796862B2 (ja) | 2015-10-21 |
| NZ578390A (en) | 2012-01-12 |
| JP5958982B2 (ja) | 2016-08-02 |
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| JP2010516277A (ja) | 2010-05-20 |
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| JP2014221059A (ja) | 2014-11-27 |
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