CN103893774A - Application of beta-amylase in extraction of panax notoginseng saponins - Google Patents
Application of beta-amylase in extraction of panax notoginseng saponins Download PDFInfo
- Publication number
- CN103893774A CN103893774A CN201410141958.2A CN201410141958A CN103893774A CN 103893774 A CN103893774 A CN 103893774A CN 201410141958 A CN201410141958 A CN 201410141958A CN 103893774 A CN103893774 A CN 103893774A
- Authority
- CN
- China
- Prior art keywords
- ethanol
- radix notoginseng
- fresh
- precipitate
- beta amylase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010019077 beta-Amylase Proteins 0.000 title claims abstract description 53
- 238000000605 extraction Methods 0.000 title claims abstract description 30
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 212
- 241000180649 Panax notoginseng Species 0.000 claims abstract description 108
- 235000003143 Panax notoginseng Nutrition 0.000 claims abstract description 108
- 238000010828 elution Methods 0.000 claims abstract description 29
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 239000000706 filtrate Substances 0.000 claims abstract description 12
- 238000001556 precipitation Methods 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims description 46
- 239000002244 precipitate Substances 0.000 claims description 33
- 239000000284 extract Substances 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 102000005744 Glycoside Hydrolases Human genes 0.000 claims description 14
- 108010031186 Glycoside Hydrolases Proteins 0.000 claims description 14
- 241000985513 Penicillium oxalicum Species 0.000 claims description 14
- 239000011347 resin Substances 0.000 claims description 14
- 229920005989 resin Polymers 0.000 claims description 14
- 238000010992 reflux Methods 0.000 claims description 12
- 239000008187 granular material Substances 0.000 claims description 11
- 230000000274 adsorptive effect Effects 0.000 claims description 7
- 239000003957 anion exchange resin Substances 0.000 claims description 7
- 230000006837 decompression Effects 0.000 claims description 7
- 238000004090 dissolution Methods 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 238000004064 recycling Methods 0.000 claims description 7
- -1 again Substances 0.000 claims description 3
- OCLXJTCGWSSVOE-UHFFFAOYSA-N ethanol etoh Chemical compound CCO.CCO OCLXJTCGWSSVOE-UHFFFAOYSA-N 0.000 claims description 2
- 239000008394 flocculating agent Substances 0.000 claims description 2
- 238000013467 fragmentation Methods 0.000 claims description 2
- 238000006062 fragmentation reaction Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 27
- 102000004190 Enzymes Human genes 0.000 abstract description 13
- 108090000790 Enzymes Proteins 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 8
- 229930182490 saponin Natural products 0.000 abstract description 8
- 150000007949 saponins Chemical class 0.000 abstract description 8
- 235000017709 saponins Nutrition 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000011161 development Methods 0.000 abstract description 5
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 229940126680 traditional chinese medicines Drugs 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 12
- 230000007062 hydrolysis Effects 0.000 description 7
- 238000006460 hydrolysis reaction Methods 0.000 description 7
- 230000002255 enzymatic effect Effects 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000005469 granulation Methods 0.000 description 5
- 230000003179 granulation Effects 0.000 description 5
- 238000010298 pulverizing process Methods 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 108010059892 Cellulase Proteins 0.000 description 4
- 229940106157 cellulase Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 238000005325 percolation Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000003181 Panax pseudoginseng Nutrition 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410141958.2A CN103893774B (en) | 2014-04-10 | 2014-04-10 | A kind of beta amylase is extracting the application in Radix Notoginseng total arasaponins |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410141958.2A CN103893774B (en) | 2014-04-10 | 2014-04-10 | A kind of beta amylase is extracting the application in Radix Notoginseng total arasaponins |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103893774A true CN103893774A (en) | 2014-07-02 |
CN103893774B CN103893774B (en) | 2016-01-06 |
Family
ID=50985554
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410141958.2A Active CN103893774B (en) | 2014-04-10 | 2014-04-10 | A kind of beta amylase is extracting the application in Radix Notoginseng total arasaponins |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN103893774B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104473765A (en) * | 2014-12-17 | 2015-04-01 | 文山学院文山三七研究院 | Forming process of pseudo-ginseng formula granules |
CN106511417A (en) * | 2016-11-18 | 2017-03-22 | 云南金三奇药业有限公司 | Method for extracting panax notoginseng stock solution |
CN108938696A (en) * | 2018-09-13 | 2018-12-07 | 四川奇格曼药业有限公司 | A kind of extracting method of arasaponin |
CN111297928A (en) * | 2020-04-22 | 2020-06-19 | 广东一力罗定制药有限公司 | Method for extracting panax notoginseng saponins |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102210732A (en) * | 2010-04-02 | 2011-10-12 | 文山三七研究院 | Method for extracting panax notoginseng saponins from fresh panax notoginseng by enzyme treatment |
CN102559506A (en) * | 2010-12-07 | 2012-07-11 | 中国农业科学院作物科学研究所 | Penicillium oxalicum and application thereof |
CN103340808A (en) * | 2013-07-30 | 2013-10-09 | 云南人羞花化妆品有限公司 | Preparation technology for pseudo-ginseng medicament night cream |
-
2014
- 2014-04-10 CN CN201410141958.2A patent/CN103893774B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102210732A (en) * | 2010-04-02 | 2011-10-12 | 文山三七研究院 | Method for extracting panax notoginseng saponins from fresh panax notoginseng by enzyme treatment |
CN102559506A (en) * | 2010-12-07 | 2012-07-11 | 中国农业科学院作物科学研究所 | Penicillium oxalicum and application thereof |
CN103340808A (en) * | 2013-07-30 | 2013-10-09 | 云南人羞花化妆品有限公司 | Preparation technology for pseudo-ginseng medicament night cream |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104473765A (en) * | 2014-12-17 | 2015-04-01 | 文山学院文山三七研究院 | Forming process of pseudo-ginseng formula granules |
CN104473765B (en) * | 2014-12-17 | 2018-12-11 | 文山学院文山三七研究院 | A kind of moulding process of Radix Notoginseng granule |
CN106511417A (en) * | 2016-11-18 | 2017-03-22 | 云南金三奇药业有限公司 | Method for extracting panax notoginseng stock solution |
CN108938696A (en) * | 2018-09-13 | 2018-12-07 | 四川奇格曼药业有限公司 | A kind of extracting method of arasaponin |
CN111297928A (en) * | 2020-04-22 | 2020-06-19 | 广东一力罗定制药有限公司 | Method for extracting panax notoginseng saponins |
CN111297928B (en) * | 2020-04-22 | 2021-10-22 | 一力制药(罗定)有限公司 | Method for extracting panax notoginseng saponins |
Also Published As
Publication number | Publication date |
---|---|
CN103893774B (en) | 2016-01-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103933092B (en) | The method of Radix Notoginseng total arasaponins in the fresh Radix Notoginseng of a kind of multiplex-enzyme extraction | |
CN101559157B (en) | Method for fractional extraction of asparagus saponin and asparagus amylose using asparagus slag | |
CN102020692B (en) | Method for extracting refined tea saponin from camellia oleifera seed cake | |
CN102048885B (en) | Extraction process for lychee seed saponin | |
CN102250195B (en) | Method for producing xanthoceraside | |
CN101829164B (en) | Biological preparation method of Hypericum perforatum L extractive | |
CN101053589B (en) | Method for extracting active constituent from Tibetan capillary | |
CN110684128B (en) | Method for extracting and refining polygonatum sibiricum polysaccharide | |
CN109645304B (en) | Preparation method of mulberry leaf health food base material | |
CN108752231B (en) | Method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenol | |
CN103893774B (en) | A kind of beta amylase is extracting the application in Radix Notoginseng total arasaponins | |
CN104189041A (en) | Method for extracting ginsenoside, and prepared ginsenoside extract | |
CN102106928B (en) | Method for preparing high-purity oil tea saponins | |
CN102210732A (en) | Method for extracting panax notoginseng saponins from fresh panax notoginseng by enzyme treatment | |
CN103013936A (en) | Method for extracting anthocyanidin by using compound enzyme, and compound enzyme preparation thereof | |
CN111297928B (en) | Method for extracting panax notoginseng saponins | |
CN102349951A (en) | Preparation method of hawthorn leaf extract | |
CN101955548A (en) | Method for enzyme-assisted extraction of fructus tribuli polysaccharide | |
CN104127451B (en) | A kind of method simultaneously extracting polyphenol, flavonoid and triterpenes from Flos Granati | |
CN102718737A (en) | Method of using roxburgh rose pulp to prepare roxburgh rose procyanidine | |
CN109369733B (en) | Method for simultaneously extracting multiple flavonoid compounds from tartary buckwheat leaves | |
CN110882285A (en) | Efficient preparation method of active substances in phellinus igniarius | |
CN105169094A (en) | Method for extracting and purifying total flavonoids from Indocalamus leaves | |
CN104069191A (en) | Extraction process of total flavonoids of polygonum criopolitanum | |
CN103520256A (en) | Preparation method of high-purity gynostemma pentaphylla total saponin for veterinary drug |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C41 | Transfer of patent application or patent right or utility model | ||
CB03 | Change of inventor or designer information |
Inventor after: Shao Sibo Inventor after: Yang Xiankun Inventor after: Shang Chaorong Inventor before: Yang Bing |
|
COR | Change of bibliographic data | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20151208 Address after: 679100 Tengchong County of Baoshan City, Yunnan Province town of Guanyin pond community and vault District No. 150 Applicant after: Tengchong County, Yunnan Province Dongfanghong Pharmaceutical Co Address before: 655000, Baita Road, Panlong District, Yunnan, the Mixc, 5, 1606, Kunming Applicant before: Yang Bing |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee | ||
CP03 | Change of name, title or address |
Address after: 679100 Tengchong, Yunnan Province Economic Development Zone, Tengchong Road, Hing Road, No. 07 Patentee after: Tengchong Yunnan Dongfanghong Pharmaceutical Co., Ltd. Address before: 679100 Tengchong County of Baoshan City, Yunnan Province town of Guanyin pond community and vault District No. 150 Patentee before: Tengchong County, Yunnan Province Dongfanghong Pharmaceutical Co |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Application of beta-amylase in extraction of panax notoginseng saponins Effective date of registration: 20160523 Granted publication date: 20160106 Pledgee: Tengchong Rural Credit Cooperative Association Pledgor: Tengchong Yunnan Dongfanghong Pharmaceutical Co., Ltd. Registration number: 2016530000002 |
|
PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model |