CN103891614B - A kind of substratum of inducing purple dendrobium protocorm differentiation - Google Patents

A kind of substratum of inducing purple dendrobium protocorm differentiation Download PDF

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Publication number
CN103891614B
CN103891614B CN201410118983.9A CN201410118983A CN103891614B CN 103891614 B CN103891614 B CN 103891614B CN 201410118983 A CN201410118983 A CN 201410118983A CN 103891614 B CN103891614 B CN 103891614B
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substratum
purple dendrobium
dendrobium protocorm
mnso
znso
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CN103891614A (en
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李旦
王俊
陈远书
潘瑶
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Southwest Forestry University
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Southwest Forestry University
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Abstract

The present invention relates to a kind of substratum of inducing purple dendrobium protocorm differentiation, belong to field of plant tissue culture technique.Its component is: KNO 3, NH 4nO 3, KH 2pO 4, MgSO 47H 2o, CaCl 22H 2o, KI, H 3bO 3, MnSO 44H 2o, ZnSO 47H 2o, Na 2moO 42H 2o, CuSO 45H 2o, CoCl 26H 2o, Na 2eDTA, FeSO 47H 2o, inositol, glycine, vitamin, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana puree, mashed potato, gac.The invention has the beneficial effects as follows: this substratum does not add hormone, and the seedling time can be shortened.

Description

A kind of substratum of inducing purple dendrobium protocorm differentiation
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to a kind of substratum of inducing purple dendrobium protocorm differentiation.
Background technology
The one class the orchid family Dendrobium Sw (Dendrobium devonianum Paxt) of purple dendrobium to be orchid with Dens-valve Dendrobium be representative, because its stem is purple, therefore is called that purple dendrobium, purple skin are blue.Shennong's Herbal is recorded: " stem of noble dendrobium, a Lin Lan.Taste is sweet, flat, nontoxic.In main wound, except numbness, the thick stomach of tonifying the five internal organs, makes light of one's life by commiting suicide and prolongs life ".Compendium of Material Medica claim the stem of noble dendrobium have " reinforcing yin essence benefit essence, thick stomach, mend in not enough, make light of one's life by commiting suicide and prolong life " effect.
The artificial culture of purple dendrobium starts from the nineties in 20th century, formed the small-scale plantation of dispersion in 2006, in recent years along with its significant medicinal health effect obtains the favor of consumers in general day by day, price constantly rose, planting scale fast development, economic benefits.
But for many years, purple dendrobium, when by protocorm differentiation, add hormone, and the seedling time is longer, seriously compromises the healthy of people more, and affects applying of purple dendrobium in the substratum of employing.Therefore, not adopting hormone to during protocorm differentiation, can also shorten the seedling time becomes the technical problem needing solution badly.
Summary of the invention
The object of this invention is to provide a kind of substratum of inducing purple dendrobium protocorm differentiation, this substratum does not add hormone, and can shorten the seedling time.
The technical solution used in the present invention is a kind of substratum of inducing purple dendrobium protocorm differentiation, and its component and content thereof are: KNO 3550 ~ 1450mg/L, NH 4nO 3495 ~ 1245mg/L, KH 2pO 450 ~ 125mg/L, MgSO 47H 2o120 ~ 275mg/L, CaCl 22H 2o135 ~ 330mg/L, KI0.45 ~ 0.83mg/L, H 3bO 33.75 ~ 9.00mg/L, MnSO 44H 2o13.5 ~ 34.5mg/L, ZnSO 47H 2o5.2 ~ 12.8mg/L, Na 2moO 42H 2o0.15 ~ 0.35mg/L, CuSO 45H 2o0.01 ~ 0.035mg/L, CoCl 26H 2o0.01 ~ 0.035mg/L, Na 2eDTA25 ~ 55mg/L, FeSO 47H 2o18 ~ 42mg/L, inositol 50 ~ 300mg/L, glycine 1.2 ~ 2.8mg/L, vitamin 0.3 ~ 0.7mg/L, pyridoxine hydrochloride 0.3 ~ 0.7mg/L, nicotinic acid 0.3 ~ 0.7mg/L, sucrose 18000 ~ 55000mg/L, agar 2500 ~ 8000mg/L, banana puree 30000 ~ 75000mg/L, mashed potato 30000 ~ 75000mg/L, gac 500 ~ 2000mg/L.
Preferably, its component and content thereof are: KNO 3850 ~ 1150mg/L, NH 4nO 3750 ~ 990mg/L, KH 2pO 475 ~ 100mg/L, MgSO 47H 2o165 ~ 250mg/L, CaCl 22H 2o180 ~ 240mg/L, KI0.55 ~ 0.79mg/L, H 3bO 35.5 ~ 7.5mg/L, MnSO 44H 2o20 ~ 27mg/L, ZnSO 47H 2o7.5 ~ 10.5mg/L, Na 2moO 42H 2o0.21 ~ 0.3mg/L, CuSO 45H 2o0.02 ~ 0.03mg/L, CoCl 26H 2o0.02 ~ 0.03mg/L, Na 2eDTA30 ~ 42mg/L, FeSO 47H 2o25 ~ 32mg/L, inositol 70 ~ 250mg/L, glycine 1.5 ~ 2.2mg/L, vitamin 0.4 ~ 0.6mg/L, pyridoxine hydrochloride 0.4 ~ 0.6mg/L, nicotinic acid 0.4 ~ 0.6mg/L, sucrose 20000 ~ 50000mg/L, agar 3000 ~ 7000mg/L, banana puree 40000 ~ 65000mg/L, mashed potato 40000 ~ 65000mg/L, gac 600 ~ 1500mg/L.
Particularly preferred, its component and content thereof are: KNO 3923mg/L, NH 4nO 3780mg/L, KH 2pO 485mg/L, MgSO 47H 2o200mg/L, CaCl 22H 2o200mg/L, KI0.685mg/L, H 3bO 37mg/L, MnSO 44H 2o25mg/L, ZnSO 47H 2o7.8mg/L, Na 2moO 42H 2o0.23mg/L, CuSO 45H 2o0.025mg/L, CoCl 26H 2o0.025mg/L, Na 2eDTA35mg/L, FeSO 47H 2o28mg/L, inositol 100mg/L, glycine 1.8mg/L, vitamin 0.5mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30000mg/L, agar 5000mg/L, banana puree 50000mg/L, mashed potato 50000mg/L, gac 1000mg/L.
The present invention also provides a kind of method of described culture medium culturing purple dendrobium protocorm, be inoculated in described substratum by purple dendrobium protocorm, being placed in intensity of illumination is 2000 ~ 2500Lux, and temperature is cultivate under the environment of 18 ~ 25 DEG C, cultivate 35 ~ 45 days.Particularly preferred, intensity of illumination is 2200Lux, and temperature is 20 DEG C, 22 DEG C, and the time of cultivation is 40 days.
Preferably, the time of described illumination is 14 hours ~ 18 hours every day.Particularly preferred, the time of illumination every day is 16 hours.
Preferably, before the inoculation of described purple dendrobium protocorm, by the sterilizing 20 minutes under the condition of 121 DEG C of described substratum.
Beneficial effect of the present invention is: apply the purple dendrobium differentiation seedling that culture medium culturing of the present invention goes out, without hormone, leaf look green, and stem is sturdy, not easily lodges, and has stronger synchronism.The seedling time is short, only need can to obtain for 35 ~ 45 days the purple dendrobium differentiation seedling of about 1cm, and than the seedling-growing time of existing 60 days, cycle time more than 1/3rd, was applicable to industrial seedling rearing operation.Basal culture medium comparatively other conventional medium simultaneously, consumption is few, cost-saving.
Embodiment
For making those skilled in the art understand production technique of the present invention and technique effect in detail, introduce application of the present invention and technique effect further with concrete production instance below.
Embodiment 1:
Take appropriate KNO 3, NH 4nO 3, KH 2pO 4, MgSO 47H 2o, CaCl 22H 2o, KI, H 3bO 3, MnSO 44H 2o, ZnSO 47H 2o, Na 2moO 42H 2o, CuSO 45H 2o, CoCl 26H 2o, Na 2eDTA, FeSO 47H 2o, inositol, glycine, vitamin, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana puree, mashed potato and gac mix, and obtain the substratum that pH is 5.
And the content of each component is in this substratum:
KNO 3923mg/L, NH 4nO 3780mg/L, KH 2pO 485mg/L, MgSO 47H 2o200mg/L, CaCl 22H 2o200mg/L, KI0.685mg/L, H 3bO 37mg/L, MnSO 44H 2o25mg/L, ZnSO 47H 2o7.8mg/L, Na 2moO 42H 2o0.23mg/L, CuSO 45H 2o0.025mg/L, CoCl 26H 2o0.025mg/L, Na 2eDTA35mg/L, FeSO 47H 2o28mg/L, inositol 100mg/L, glycine 1.8mg/L, vitamin 0.5mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30000mg/L, agar 5000mg/L, banana puree 50000mg/L, mashed potato 50000mg/L, gac 1000mg/L.
Embodiment 2:
Take appropriate KNO 3, NH 4nO 3, KH 2pO 4, MgSO 47H 2o, CaCl 22H 2o, KI, H 3bO 3, MnSO 44H 2o, ZnSO 47H 2o, Na 2moO 42H 2o, CuSO 45H 2o, CoCl 26H 2o, Na 2eDTA, FeSO 47H 2o, inositol, glycine, vitamin, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana puree, mashed potato and gac mix, and obtain the substratum that pH is 6.
And the content of each component is in this substratum:
KNO 31450mg/L, NH 4nO 3495mg/L, KH 2pO 450mg/L, MgSO 47H 2o275mg/L, CaCl 22H 2o330mg/L, KI0.83mg/L, H 3bO 39.00mg/L, MnSO 44H 2o34.5mg/L, ZnSO 47H 2o12.8mg/L, Na 2moO 42H 2o0.35mg/L, CuSO 45H 2o0.035mg/L, CoCl 26H 2o0.035mg/L, Na 2eDTA55mg/L, FeSO 47H 2o42mg/L, inositol 300mg/L, glycine 2.8mg/L, vitamin 0.7mg/L, pyridoxine hydrochloride 0.7mg/L, nicotinic acid 0.7mg/L, sucrose 55000mg/L, agar 8000mg/L, banana puree 75000mg/L, mashed potato 75000mg/L, gac 2000mg/L.
Embodiment 3:
Take appropriate KNO 3, NH 4nO 3, KH 2pO 4, MgSO 47H 2o, CaCl 22H 2o, KI, H 3bO 3, MnSO 44H 2o, ZnSO 47H 2o, Na 2moO 42H 2o, CuSO 45H 2o, CoCl 26H 2o, Na 2eDTA, FeSO 47H 2o, inositol, glycine, vitamin, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana puree, mashed potato and gac mix, and obtain the substratum that pH is 5.5.
And the content of each component is in this substratum: KNO 3550mg/L, NH 4nO 31245mg/L, KH 2pO 4125mg/L, MgSO 47H 2o120mg/L, CaCl 22H 2o135mg/L, KI0.45mg/L, H 3bO 33.75mg/L, MnSO 44H 2o13.5mg/L, ZnSO 47H 2o5.2mg/L, Na 2moO 42H 2o0.15mg/L, CuSO 45H 2o0.01mg/L, CoCl 26H 2o0.01mg/L, Na 2eDTA25mg/L, FeSO 47H 2o18mg/L, inositol 50mg/L, glycine 1.2mg/L, vitamin 0.3mg/L, pyridoxine hydrochloride 0.3mg/L, nicotinic acid 0.3mg/L, sucrose 18000mg/L, agar 2500mg/L, banana puree 30000mg/L, mashed potato 30000mg/L, gac 500mg/L.
Embodiment 4:
Take appropriate KNO 3, NH 4nO 3, KH 2pO 4, MgSO 47H 2o, CaCl 22H 2o, KI, H 3bO 3, MnSO 44H 2o, ZnSO 47H 2o, Na 2moO 42H 2o, CuSO 45H 2o, CoCl 26H 2o, Na 2eDTA, FeSO 47H 2o, inositol, glycine, vitamin, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana puree, mashed potato and gac mix, and obtain the substratum that pH is 5.2.
And the content of each component is in this substratum: KNO 3850mg/L, NH 4nO 3750mg/L, KH 2pO 475mg/L, MgSO 47H 2o165mg/L, CaCl 22H 2o180mg/L, KI0.55mg/L, H 3bO 35.5mg/L, MnSO 44H 2o20mg/L, ZnSO 47H 2o7.5mg/L, Na 2moO 42H 2o0.21mg/L, CuSO 45H 2o0.02mg/L, CoCl 26H 2o0.02mg/L, Na 2eDTA30mg/L, FeSO 47H 2o25mg/L, inositol 70mg/L, glycine 1.5mg/L, vitamin 0.4mg/L, pyridoxine hydrochloride 0.4mg/L, nicotinic acid 0.4mg/L, sucrose 20000mg/L, agar 3000mg/L, banana puree 40000mg/L, mashed potato 40000mg/L, gac 600mg/L.
Embodiment 5:
Take appropriate KNO 3, NH 4nO 3, KH 2pO 4, MgSO 47H 2o, CaCl 22H 2o, KI, H 3bO 3, MnSO 44H 2o, ZnSO 47H 2o, Na 2moO 42H 2o, CuSO 45H 2o, CoCl 26H 2o, Na 2eDTA, FeSO 47H 2o, inositol, glycine, vitamin, pyridoxine hydrochloride, nicotinic acid, sucrose, agar, banana puree, mashed potato and gac mix, and obtain the substratum that pH is 5.8.
And the content of each component is in this substratum: KNO 31150mg/L, NH 4nO 3990mg/L, KH 2pO 4100mg/L, MgSO 47H 2o250mg/L, CaCl 22H 2o240mg/L, KI0.79mg/L, H 3bO 37.5mg/L, MnSO 44H 2o27mg/L, ZnSO 47H 2o10.5mg/L, Na 2moO 42H 2o0.3mg/L, CuSO 45H 2o0.03mg/L, CoCl 26H 2o0.03mg/L, Na 2eDTA42mg/L, FeSO 47H 2o32mg/L, inositol 250mg/L, glycine 2.2mg/L, vitamin 0.6mg/L, pyridoxine hydrochloride 0.6mg/L, nicotinic acid 0.6mg/L, sucrose 50000mg/L, agar 7000mg/L, banana puree 65000mg/L, mashed potato 65000mg/L, gac 1500mg/L.
For verifying the technique effect of substratum of the present invention, by the sterilizing 20 minutes under the condition of 121 DEG C of the substratum of the various embodiments described above gained, then purple dendrobium protocorm is inoculated in substratum by spy, being placed in intensity of illumination is 2000 ~ 2500Lux, temperature is cultivate under the environment of 18 ~ 25 DEG C, cultivate 35 ~ 45 days, the time of illumination is 14 hours ~ 18 hours every day, can obtain the purple dendrobium differentiation seedling of about 1cm.Compare existing substratum or the technical scheme not in the component and content thereof of the claimed scope of the present patent application, the seedling time shortens greatly, cost reduction; and without hormone, leaf look green, and stem is sturdy; not easily lodge, there is stronger synchronism, there is significant technical progress.
Finally it should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although with reference to above-described embodiment to invention has been detailed description, those skilled in the art are to be understood that, still can modify to the present invention or equivalent replacement, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in right of the present invention.

Claims (6)

1. induce a substratum for purple dendrobium protocorm differentiation, it is characterized in that: its component and content thereof are: KNO 3550 ~ 1450mg/L, NH 4nO 3495 ~ 1245mg/L, KH 2pO 450 ~ 125mg/L, MgSO 47H 2o120 ~ 275mg/L, CaCl 22H 2o135 ~ 330mg/L, KI0.45 ~ 0.83mg/L, H 3bO 33.75 ~ 9.00mg/L, MnSO 44H 2o13.5 ~ 34.5mg/L, ZnSO 47H 2o5.2 ~ 12.8mg/L, Na 2moO 42H 2o0.15 ~ 0.35mg/L, CuSO 45H 2o0.01 ~ 0.035mg/L, CoCl 26H 2o0.01 ~ 0.035mg/L, Na 2eDTA25 ~ 55mg/L, FeSO 47H 2o18 ~ 42mg/L, inositol 50 ~ 300mg/L, glycine 1.2 ~ 2.8mg/L, vitamin 0.3 ~ 0.7mg/L, pyridoxine hydrochloride 0.3 ~ 0.7mg/L, nicotinic acid 0.3 ~ 0.7mg/L, sucrose 18000 ~ 55000mg/L, agar 2500 ~ 8000mg/L, banana puree 30000 ~ 75000mg/L, mashed potato 30000 ~ 75000mg/L, gac 500 ~ 2000mg/L.
2. a kind of substratum of inducing purple dendrobium protocorm differentiation according to claim 1, is characterized in that: its component and content thereof are: KNO 3850 ~ 1150mg/L, NH 4nO 3750 ~ 990mg/L, KH 2pO 475 ~ 100mg/L, MgSO 47H 2o165 ~ 250mg/L, CaCl 22H 2o180 ~ 240mg/L, KI0.55 ~ 0.79mg/L, H 3bO 35.5 ~ 7.5mg/L, MnSO 44H 2o20 ~ 27mg/L, ZnSO 47H 2o7.5 ~ 10.5mg/L, Na 2moO 42H 2o0.21 ~ 0.3mg/L, CuSO 45H 2o0.02 ~ 0.03mg/L, CoCl 26H 2o0.02 ~ 0.03mg/L, Na 2eDTA30 ~ 42mg/L, FeSO 47H 2o25 ~ 32mg/L, inositol 70 ~ 250mg/L, glycine 1.5 ~ 2.2mg/L, vitamin 0.4 ~ 0.6mg/L, pyridoxine hydrochloride 0.4 ~ 0.6mg/L, nicotinic acid 0.4 ~ 0.6mg/L, sucrose 20000 ~ 50000mg/L, agar 3000 ~ 7000mg/L, banana puree 40000 ~ 65000mg/L, mashed potato 40000 ~ 65000mg/L, gac 600 ~ 1500mg/L.
3. a kind of substratum of inducing purple dendrobium protocorm differentiation according to claim 2, is characterized in that: its component and content thereof are: KNO 3923mg/L, NH 4nO 3780mg/L, KH 2pO 485mg/L, MgSO 47H 2o200mg/L, CaCl 22H 2o200mg/L, KI0.685mg/L, H 3bO 37mg/L, MnSO 44H 2o25mg/L, ZnSO 47H 2o7.8mg/L, Na 2moO 42H 2o0.23mg/L, CuSO 45H 2o0.025mg/L, CoCl 26H 2o0.025mg/L, Na 2eDTA35mg/L, FeSO 47H 2o28mg/L, inositol 100mg/L, glycine 1.8mg/L, vitamin 0.5mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30000mg/L, agar 5000mg/L, banana puree 50000mg/L, mashed potato 50000mg/L, gac 1000mg/L.
4. one kind utilizes the method for culture medium culturing purple dendrobium protocorm described in any one of claim 1-3, it is characterized in that: purple dendrobium protocorm is inoculated in described substratum, being placed in intensity of illumination is 2000 ~ 2500Lux, temperature is cultivate under the environment of 18 ~ 25 DEG C, cultivates 35 ~ 45 days.
5. the method for culture medium culturing purple dendrobium protocorm according to claim 4, is characterized in that: the time of described illumination is 14 hours ~ 18 hours every day.
6. the method for culture medium culturing purple dendrobium protocorm according to claim 5, is characterized in that: before described purple dendrobium protocorm inoculation, by the sterilizing 20 minutes under the condition of 121 DEG C of described substratum.
CN201410118983.9A 2014-03-27 2014-03-27 A kind of substratum of inducing purple dendrobium protocorm differentiation Expired - Fee Related CN103891614B (en)

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CN101889547A (en) * 2009-05-22 2010-11-24 云南省德宏热带农业科学研究所 Aseptic and rapid propagation method of dendrobium devonianum seeds

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