CN103882079A - Spinosad fermentation medium formula - Google Patents
Spinosad fermentation medium formula Download PDFInfo
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- CN103882079A CN103882079A CN201210553282.9A CN201210553282A CN103882079A CN 103882079 A CN103882079 A CN 103882079A CN 201210553282 A CN201210553282 A CN 201210553282A CN 103882079 A CN103882079 A CN 103882079A
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- spinosad
- fermentation medium
- pleocidin
- medium formula
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Abstract
A disclosed spinosad fermentation medium formula comprises the following compositions: 50.0 g/L of glucose, 25.0 g/L of cottonseed protein, 20.0 g/L of corn peptone, 0.8 g/L of ammonium sulfate, 1.25 g/L of glutamine, 0.8 g/L of sodium acetate, 3.0 g/L of sodium chloride, 2.5 g/L of dipotassium hydrogen phosphate, 50.0 mg/L of ferrous sulphate, 25.0 mg/L of zinc chloride, and 1.0 g/L of calcium carbonate. The fermentation medium is prepared by uniformly mixing the above compositions. Spinosad prepared by employing the fermentation medium is low in solubility in water and easy to dissolve in organic solvents, spinosad-producing strain is substantially improved on conventional fermentation level, and the domestic industrialization of spinosad is facilitated.
Description
Technical field
The invention belongs to the formula of fermention medium, be specifically related to a kind of pleocidin fermentative medium formula.
Background technology
Pleocidin is the interior secondary metabolite of born of the same parents of actinomycetes, thorn saccharopolyspora strain aerobic fermentation, and its activeconstituents is A83543A and A83543D, all belongs to macrolides compound.Pleocidin can effectively be prevented and treated most lepidopterans, Orthoptera, Diptera and coleopteran pest, because of its have insecticidal spectrum wide, active high, to non-target animal the harmless and degradation production advantage such as safely.At present, domestic have the research report that utilizes carbon nitrogen source, inorganic salt and precursor situation about thorn saccharopolyspora strain, but few to the research of thorn saccharopolyspora strain fermenting process metabolism situation, on the low side to the investigation level of fermention medium one-component.In addition, genetic background and the metabolic characteristic of different strains are different, and the plain fermention medium of optimum product is also often different.
Summary of the invention
The problems referred to above that exist in order to overcome prior art field, the object of the invention is to, a kind of pleocidin fermentative medium formula is provided, solves few, on the low side to the investigation level of the fermention medium one-component problem of research to thorn saccharopolyspora strain fermenting process metabolism situation at present.
Pleocidin fermentative medium formula provided by the invention, it comprises each component of following weight: glucose 50.0g/L, cottonseed protein 25.0g/L, zein peptone 20.0g/L, ammonium sulfate 0.8g/L, glutamine 1.25g/L, sodium acetate 0.8g/L, sodium-chlor 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, ferrous sulfate 50.0mg/L, zinc chloride 25.0mg/L, calcium carbonate 1.0g/L, the even mixed preparing of above each component forms.
Pleocidin fermentative medium formula provided by the invention, its beneficial effect is, this substratum pleocidin made solubleness in water of fermenting is low, be soluble in organic solvent, utilize this fermention medium, can make pleocidin producing bacteria greatly improve on original fermentation level basis, be conducive to the industrialization of domestic pleocidin.
Embodiment
Below in conjunction with embodiment, to pleocidin fermentative medium formula provided by the invention, be described in detail.
Embodiment
The pleocidin fermentative medium formula of the present embodiment, it comprises each component of following weight: glucose 50.0g/L, cottonseed protein 25.0g/L, zein peptone 20.0g/L, ammonium sulfate 0.8g/L, glutamine 1.25g/L, sodium acetate 0.8g/L, sodium-chlor 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, ferrous sulfate 50.0mg/L, zinc chloride 25.0mg/L, calcium carbonate 1.0g/L, the even mixed preparing of above each component forms.
The pleocidin output of this formula reaches 395.54Lg/mL, and contrast improves 62.5%.
Claims (1)
1. a pleocidin fermentative medium formula, it is characterized in that: it comprises each component of following weight: glucose 50.0g/L, cottonseed protein 25.0g/L, zein peptone 20.0g/L, ammonium sulfate 0.8g/L, glutamine 1.25g/L, sodium acetate 0.8g/L, sodium-chlor 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, ferrous sulfate 50.0mg/L, zinc chloride 25.0mg/L, calcium carbonate 1.0g/L, the even mixed preparing of above each component forms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210553282.9A CN103882079A (en) | 2012-12-19 | 2012-12-19 | Spinosad fermentation medium formula |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210553282.9A CN103882079A (en) | 2012-12-19 | 2012-12-19 | Spinosad fermentation medium formula |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103882079A true CN103882079A (en) | 2014-06-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210553282.9A Pending CN103882079A (en) | 2012-12-19 | 2012-12-19 | Spinosad fermentation medium formula |
Country Status (1)
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| CN (1) | CN103882079A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105506038A (en) * | 2014-09-26 | 2016-04-20 | 牡丹江佰佳信生物科技有限公司 | Method for producing pleocidin by adopting saccharopolyspora spinosa through fermentation |
| CN115011526A (en) * | 2022-07-08 | 2022-09-06 | 黄河三角洲京博化工研究院有限公司 | Fermentation medium of pleocidin and production method of pleocidin |
-
2012
- 2012-12-19 CN CN201210553282.9A patent/CN103882079A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105506038A (en) * | 2014-09-26 | 2016-04-20 | 牡丹江佰佳信生物科技有限公司 | Method for producing pleocidin by adopting saccharopolyspora spinosa through fermentation |
| CN115011526A (en) * | 2022-07-08 | 2022-09-06 | 黄河三角洲京博化工研究院有限公司 | Fermentation medium of pleocidin and production method of pleocidin |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140625 |