CN103881150B - A kind of natural polymer/inorganic nano antiseptic composite and preparation method thereof - Google Patents
A kind of natural polymer/inorganic nano antiseptic composite and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of natural polymer/inorganic nano antiseptic composite and preparation method thereof.This kind of natural polymer/inorganic nano antiseptic composite, it is characterised in that: mixed by nano silver colloidal sol and nano-zinc oxide sol.Its preparation method comprises the following steps: the preparation of (1) nano silver colloidal sol；(2) preparation of nano-zinc oxide sol；(3) preparation of natural polymer/inorganic nano antiseptic composite: step (1) gained nano silver colloidal sol is mixed with step (2) gained nano-zinc oxide sol 1:2 1:3 by volume, obtains natural polymer/inorganic nano antiseptic composite.Natural polymer prepared by the present invention/inorganic nano antiseptic composite good anti-bacterial effect, antibacterial timeliness length and nontoxic.
Description
Technical field
The invention belongs to functional natural macromolecular material field, natural particularly to one
Macromolecule/inorganic nano antiseptic composite and preparation method thereof.
Background technology
Along with expanding economy, the raising of living standards of the people, comfortable joyful pursuing
While life style, also look forward to having that one comfortable, health, the living environment of safety.
Healthy air, healthy water source, healthy food, healthy house etc. all represent
The new trend of current product.Noxious bacteria always affects the master in human health and life-span
Want factor.The raising realized along with national life level and health environment, nano-antibacterial material
The demand of material is also gradually increased, and the research of nano anti-biotic material will become important with preparation
New industry field, nano anti-biotic material has weight to creation clean environment, health environment
Act on.
Traditional anti-biotic material uses lead salt, mercury salt, hydrochlorate etc., toxicity own is very big,
The longevity of residure is longer, has been prohibited from using；Although and using synthesis organic bactericide consumption
Few, effect is relatively good, but also exist that effect duration is short, toxicity big, poor heat resistance,
Poor chemical stability, easily the shortcoming such as develop immunity to drugs.Some inorganic nano-particle is such as received
Rice ZnO, nanometer Ag etc. because of have good anti-bacterial effect, effect length, spectrum anti-/ sterilize with
And to advantages such as humans and animals avirulences, have begun to use in some high-grade paints,
For preparing antimicrobial nano composite environmental-friendly coating.
Owing to nanoparticle has stronger skin effect, so using traditional method to prepare
Nano anti-biotic material after placing for a long time, there will be nanoparticle agglomeration, directly
Connect the antibacterial effect having influence on nano anti-biotic material.
Summary of the invention
The technical problem to be solved be for present in prior art above-mentioned not
Foot, it is provided that a kind of natural polymer/inorganic nano antiseptic composite and preparation method thereof,
It is with low cost, preparation method is easy, and the natural polymer/inorganic nano prepared resists
Bacterium composite good anti-bacterial effect, antibacterial timeliness length, nontoxic to people and animals.
Solve the technology of the present invention problem and be employed technical scheme comprise that this natural polymer/nothing
Machine nano-antibacterial composite material is mixed by nano silver colloidal sol and nano-zinc oxide sol.
Silver-series antibacterial agent is that the effect of microorganism is obtained anti-by the silver ion by dissolution type
Bacterium characteristic, it has a good anti-microbial property, but nanometer silver is easily reunited and made its timeliness
Property is limited.Natural polymer prepared by the present invention/inorganic nano antiseptic composite is molten
Glue form, can effectively prevent the reunion of nanoparticle, prepare antibacterial effect excellent,
The nano-antibacterial composite material that good dispersion, stability are high.
Preferably, in described nano silver colloidal sol nanometer silver concentration be 80-1.12 ×
103mg/L；In described nano-zinc oxide sol, zinc oxide concentration is 4 × 103-5×103
mg/L；Described nano silver colloidal sol and nano-zinc oxide sol volume ratio are 1:2-1:3.
Present invention also offers above-mentioned natural polymer/inorganic nano antiseptic composite
Preparation method, it comprises the following steps:
(1) preparation of nano silver colloidal sol: Resina persicae is made peach glue colloidal sol, then to Resina persicae
The hydrosol adds soluble inorganic Ag-containing compound, stirs to soluble inorganic under room temperature
Ag-containing compound is completely dissolved, then containing silver sol, this is heated to 100-120 DEG C, reaction
3-4h, and be cooled to room temperature, dilute, obtain concentration of silver ions be 80-1.12 ×
103Mg/L containing silver sol；
(2) preparation of nano-zinc oxide sol: under room temperature, by 3-3.5 parts by weight acetic acid,
0.2-0.4 weight portion chitosan is dissolved in 100 weight parts waters, adds 11-13 weight portion
Carbamide, adds 20-23 weight portion soluble zinc salt, adds after stirring and dissolving after stirring and dissolving
Heat, to 75-85 DEG C, reacts 3-6h, and dilute, and obtaining zinc oxide concentration is 4 × 103-5
×103The nano-zinc oxide sol of mg/L；
(3) preparation of natural polymer/inorganic nano antiseptic composite: by step (1)
Gained nano silver colloidal sol and step (2) gained nano-zinc oxide sol 1:2-1:3 by volume
Mixing, obtains natural polymer/inorganic nano antiseptic composite.
In above-mentioned steps (1), Resina persicae simultaneously works as the effect of reducing agent and stabilizer, and one
Aspect, with aldehyde radical and the glucide of hydroxyl and soluble inorganic argentiferous chemical combination in Resina persicae
Thing generation redox reaction generates nanometer silver；On the other hand, nano silver particles attachment or
It is wrapped in Resina persicae the long chain structure of polysaccharide and a small amount of protein not participating in reaction
On, play stable effect.In step (2), chitosan is used as dispersant, and carbamide is used as
Reducing agent and zinc acetate generation redox reaction prepare nano zine oxide.Chitosan used
Itself also has anti-microbial property.
Preferably, step (1) described peach glue colloidal sol is dissolved in water by Resina persicae, removes miscellaneous
Matter, and drying, pulverization process, cross the sieve of 250 mesh, obtain Resina persicae powder, then
3 × 10 are obtained by soluble in water for Resina persicae powder3-7×103The solution of mg/L, centrifuging and taking upper strata
Clear liquid obtains.
It may further be preferable that described being dried is placed in baking oven in 60 DEG C of dry 36h；
Described centrifugal it be placed in centrifuge 3000 and leave heart 2min.
Preferably, step (1) described soluble inorganic Ag-containing compound includes silver nitrate
Or silver sulfate.
Preferably, step (2) described soluble zinc salt is Zn (CH3COO)2·2H2O
Or ZnSO4·7H2O。
It should be noted that described room temperature is 10-30 DEG C.
Natural polymer prepared by the present invention/inorganic nano antiseptic composite can directly be used
Make bactericidal liquid, take debita spissitudo and can kill more than 99% escherichia coli, golden yellow Portugal
The noxious bacteria such as grape coccus.
The rosaceous plant trunks such as Resina persicae system Fructus Persicae or Prunus davidiana by mechanical injury or cause a disease after secret out of
The colloid vitrina come, the main component in Resina persicae is polysaccharide, protein etc., tool
There is certain sterilization protective effect.The monosaccharide of composition peach gum polysaccharide mainly by arabinose and
Galactose forms, additionally possibly together with a small amount of rhamnose, mannose etc., and can be as reduction
Agent and stabilizer use.Resina persicae aboundresources, cheap and easy to get, but by Resina persicae industrialization
The research utilized, the most still at an early stage.
It addition, natural polymer of the present invention/inorganic nano antiseptic composite can be used as
Antibacterial additives, is used for preparing the chemical products such as antibiotic paint, textile, detergent.
The invention has the beneficial effects as follows: efficiently solve nanometer silver antimicrobial in prior art
Agent timeliness is short, the problem of cost intensive, utilizes Resina persicae to prepare good anti-bacterial effect, resist
Bacterium timeliness length, nontoxic natural polymer/inorganic nano antiseptic composite.Test
Result shows, escherichia coli, staphylococcus aureus are all had by this antimicrobial composite material
Good killing effect.
Accompanying drawing explanation
Fig. 1 is that the natural polymer/inorganic nano antiseptic prepared by the embodiment of the present invention one is multiple
The photo that escherichia coli minimal bactericidal concentration (MBC) is tested by condensation material；
Fig. 2 is that the natural polymer/inorganic nano antiseptic prepared by the embodiment of the present invention one is multiple
Staphylococcus aureus minimal bactericidal concentration (MBC) is tested photo by condensation material；
Fig. 3 is that the natural polymer/inorganic nano antiseptic prepared by the embodiment of the present invention one is multiple
Escherichia coli, staphylococcus aureus minimum inhibitory concentration (MIC) are surveyed by condensation material
Examination photo；
Fig. 4 is that the natural polymer/inorganic nano antiseptic prepared by the embodiment of the present invention two is multiple
Escherichia coli, staphylococcus aureus minimum inhibitory concentration (MIC) are surveyed by condensation material
Examination photo；
Fig. 5 is that the natural polymer/inorganic nano antiseptic prepared by the embodiment of the present invention four is multiple
Escherichia coli, staphylococcus aureus minimum inhibitory concentration (MIC) are surveyed by condensation material
Examination photo.
Detailed description of the invention
For making those skilled in the art be more fully understood that technical scheme, knot below
Close accompanying drawing the present invention is described in further detail.
Resina persicae of the present invention is commercially available natural Resina persicae.
The large intestine that strain subject used by the embodiment of the present invention provides for Central-South hospital of Wuhan University
Angstrom uncommon bacterium (ATCC25922), staphylococcus aureus (ATCC25923), antibacterial
Purification inoculates ordinary nutritional fluid medium after cultivating.
Embodiment one
100g Resina persicae is dissolved in 1L distilled water, after Resina persicae is swelling, removes residual bark
Deng impurity, then residue peach glue colloidal sol is put in 60 DEG C of dry 36h in baking oven, be dried
After it is pulverized with pulverizer, and by the sieve of 250 mesh, after sieving, obtain particle diameter
Resina persicae powder less than 3 μm.Take Resina persicae powder 2g and be dissolved in 400mL water, treat the most molten
Xie Hou, is placed in centrifuge 3000 and leaves heart 2min, take the supernatant and obtain
360mL peach glue colloidal sol.
Take addition 0.048g silver nitrate, room temperature (25 in above-mentioned peach glue colloidal sol 200mL
DEG C) under stir, until its dissolve after put into heated and stirred in magnetic force heating stirrer, temperature
It is set as 100 DEG C, reacts 3 hours, be cooled to room temperature, and dilute, obtain concentration
Golden yellow nano silver colloidal sol for 168mg/L.
In 100mL distilled water, add acetic acid and the 0.25g chitosan of 3mL, treat that it is complete
Add 12g carbamide after CL, add 21.95g zinc acetate, after it is completely dissolved,
Solution is imported in the there-necked flask of 250mL, 80 DEG C be slowly stirred in the case of be incubated 4 little
Time, obtaining the leucosol containing flocculence material, room temperature stands transparent colloidal sol after 12h,
Dilute, obtaining concentration is 4.575 × 103Mg/L nano-zinc oxide sol.
Above-mentioned prepared nano silver colloidal sol and nano-zinc oxide sol 1:2 by volume are mixed
Close, prepare natural polymer/inorganic nano antiseptic composite.
The double dilution method of test tube is used to measure the minimum inhibitory concentration of above-mentioned antimicrobial composite material
(MIC) and minimum bactericidal concentration (MBC), detection method is as follows:
1. the mensuration of minimum inhibitory concentration (MIC)
The natural polymer that the present embodiment is prepared/inorganic nano antiseptic composite
The antimicrobial fluid of the variable concentrations obtained after doubling dilution is added separately to 20 hole polychlorostyrene second of sterilizing
In alkene ELISA Plate, the 1st to the 9th hole of upper and lower two rows adds antimicrobial fluid, every hole 10 μ L,
10th hole is not added with antibacterial as growth control, seals, less than-20 DEG C after lyophilization
Save backup.
The escherichia coli (ATCC25922) of 18 to 24h, golden yellow Fructus Vitis viniferae will be cultivated
Coccus (ATCC25923) bacterium colony is deployed into the bacteria suspension of 0.5 Maxwell standard opacity tube,
By the standard bacteria suspension of preparation after 1:1000 dilutes, in above-mentioned every hole, add 100 μ L,
Seal in rearmounted 35 DEG C of common biochemical cultivation cases, cultivate judged result after 24h.Pass through mesh
Survey, little for MIC(with the minimum antimicrobial fluid concentration completely inhibiting bacterial growth in aperture
The transparent explanation in hole good anti-bacterial effect, bacterial clump does not grow；Occur that muddiness has then illustrated
Growth).
2. minimal bactericidal concentration (MBC) measures
The escherichia coli (ATCC25922) of 18 to 24h, golden yellow Fructus Vitis viniferae will be cultivated
Coccus (ATCC25923) bacterium colony is deployed into the bacteria suspension of 0.5 Maxwell standard opacity tube,
With nutrient broth, bacteria suspension good for above-mentioned configuration is carried out 1:1000 dilution and wait standby.Take
Concentration is 1.5 × 106The bacteria suspension 0.2mL of individual/mL is seeded to 1.8mL variable concentrations
Antimicrobial fluid and do not add antimicrobial composite material as comparison culture medium in, streak inoculation is common
Cultivate on nutrient agar panel, observe after 37 DEG C of cultivation 24h and judge the antibacterial of variable concentrations
The liquid antibacterial effect to two kinds of bacterium.Diluted concentration and anti-bacteria test result are as shown in the table:
Note :+represent that there is sterilization effect；-represent there is no sterilization effect
Anti-biotic material to the measurement result of the antibacterial experiment of two kinds of bacterium the most as shown in Figure 1, Figure 2,
Fig. 3.Fig. 1 is escherichia coli growing state in variable concentrations antimicrobial fluid, from 1
To No. 10, the content of nanoparticle is fewer and feweri, and No. 5 is that the minimum of escherichia coli kills
Bacteria concentration, now nanometer silver and nano oxidized zinc concentration be respectively 0.7mg/L and
38.125mg/L.Fig. 2 is staphylococcus aureus growth in variable concentrations antimicrobial fluid
Situation, from 1 to No. 10, the content of nanoparticle is fewer and feweri, and No. 7 is that large intestine angstrom is wished
The minimum bactericidal concentration of bacterium, now nanometer silver and nano oxidized zinc concentration are respectively
0.175mg/L and 9.5313mg/L.According to Fig. 3 antibacterial test MIC effect photo (on
Row escherichia coli, lower row staphylococcus aureus), for escherichia coli, antibacterial multiple
There is muddiness to solution during 1:64 in condensation material concentration dilution, and now nanometer silver concentration is
0.35mg/L, nano oxidized zinc concentration is 19.0625mg/L.For staphylococcus aureus,
There is muddiness, now nanometer silver concentration to solution during 1:256 in antimicrobial composite material concentration dilution
For 0.0875mg/L, nano oxidized zinc concentration is 4.765mg/L.
By measurement result find obtained by natural polymer/inorganic nano antiseptic be combined
Material is to escherichia coli (ATCC25922), staphylococcus aureus (ATCC25923)
There is good antibacterial effect.
Embodiment two
100g Resina persicae is dissolved in 1L distilled water, after Resina persicae is swelling, removes residual bark
Deng impurity, then residue peach glue colloidal sol is put in 60 DEG C of dry 36h in baking oven, be dried
After it is pulverized with pulverizer, and by the sieve of 250 mesh, after sieving, obtain particle diameter
Resina persicae powder less than 3 μm.Take Resina persicae powder 2g and be dissolved in 400mL water, treat the most molten
Xie Hou, is placed in centrifuge 3000 and leaves heart 2min, take the supernatant and obtain
360mL peach glue colloidal sol.
Take addition 0.024g silver nitrate, room temperature 25 DEG C in above-mentioned peach glue colloidal sol 200mL
Lower stirring, puts into heated and stirred in magnetic force heating stirrer after it dissolves, and temperature sets
Being 100 DEG C, react 3 hours, be cooled to room temperature, it is 84mg/L that dilute obtains concentration
Golden yellow nano silver colloidal sol.
In 100mL distilled water, add acetic acid and the 0.4g chitosan of 3.3mL, treat it
Add 13g carbamide after being completely dissolved, add 23g zinc acetate, after it is completely dissolved,
Solution is imported in the there-necked flask of 250mL, 80 DEG C be slowly stirred in the case of be incubated 4 little
Time, obtaining the leucosol containing flocculence material, room temperature stands transparent colloidal sol after 12h,
Dilute, obtaining concentration is 4.778 × 103Mg/L nano-zinc oxide sol.
Above-mentioned prepared nano silver colloidal sol and nano-zinc oxide sol 1:2 by volume are mixed
Close, prepare natural polymer/inorganic nano antiseptic composite.
Use natural polymer that the present embodiment prepared by the method identical with embodiment one/
Inorganic nano antiseptic composite is tested: the minimum bactericidal concentration of escherichia coli is
Nanometer silver and nano oxidized zinc concentration are respectively 0.7mg/L and 38.125mg/L；Golden yellow
The staphylococcic minimum bactericidal concentration of color is 0.7mg/L and 38.125mg/L.Such as Fig. 4,
Antibacterial test MIC photo (above arranging escherichia coli, lower row staphylococcus aureus) shows,
For escherichia coli, during antimicrobial composite material concentration dilution to 1:64, there is muddiness in solution,
For staphylococcus aureus, during antimicrobial composite material concentration dilution to 1:64, solution occurs
Muddiness, and when concentration is more than 1:64 to escherichia coli (ATCC25922), golden yellow
Color staphylococcus (ATCC25923) has good antibacterial effect.
Embodiment three
100g Resina persicae is dissolved in 1L distilled water, after Resina persicae is swelling, removes residual bark
Deng impurity, then residue peach glue colloidal sol is put in 60 DEG C of dry 36h in baking oven, be dried
After it is pulverized with pulverizer, and by the sieve of 250 mesh, after sieving, obtain particle diameter
Resina persicae powder less than 3 μm.Take Resina persicae powder 2g and be dissolved in 100mL water, treat the most molten
Xie Hou, is placed in centrifuge 3000 and leaves heart 2min, take the supernatant and obtain 90mL
Peach glue colloidal sol.
In above-mentioned peach glue colloidal sol, add 0.144g silver nitrate, stir at room temperature 25 DEG C,
Putting into heated and stirred in magnetic force heating stirrer after it dissolves, temperature is set as 100 DEG C,
React 3 hours, be cooled to room temperature, and dilute, obtain containing a little red-brown precipitation
Concentration is the rufous nano silver colloidal sol of 1120mg/L.
In 100mL distilled water, add acetic acid and the 0.2g chitosan of 2.85mL, treat it
Add 11g carbamide after being completely dissolved, add 20g zinc acetate, after it is completely dissolved,
Solution is imported in the there-necked flask of 250mL, 80 DEG C be slowly stirred in the case of be incubated 4 little
Time, obtaining the leucosol containing flocculence material, room temperature stands transparent colloidal sol after 12h,
Dilute, obtaining concentration is 4.175 × 103Mg/L nano-zinc oxide sol.
Above-mentioned prepared nano silver colloidal sol and nano-zinc oxide sol 1:3 by volume are mixed
Close, prepare natural polymer/inorganic nano antiseptic composite.
Use natural polymer that the present embodiment prepared by the method identical with embodiment one/
Inorganic nano antiseptic composite is tested, and test result shows: escherichia coli
Minimum bactericidal concentration is nanometer silver and nano oxidized zinc concentration be respectively 11.2mg/L and
610mg/L.The minimum bactericidal concentration of staphylococcus aureus is nanometer silver and nano zine oxide
Concentration be respectively 5.6mg/L and 305mg/L.Antibacterial test MIC shows: for greatly
The uncommon bacterium of intestinal angstrom, during antimicrobial composite material concentration dilution to 1:8, there is muddiness in solution, for gold
Staphylococcus aureus, during antimicrobial composite material concentration dilution to 1:8, there is muddiness in solution.
Embodiment four
100g Resina persicae is dissolved in 1L distilled water, after Resina persicae is swelling, removes residual bark
Deng impurity, then residue peach glue colloidal sol is put in 60 DEG C of dry 36h in baking oven, be dried
After it is pulverized with pulverizer, and by the sieve of 250 mesh, after sieving, obtain particle diameter
Resina persicae powder less than 3 μm.Take Resina persicae powder 1g and be dissolved in 400mL water, obtain Resina persicae
The hydrosol.Until completely dissolved, it is placed in centrifuge 3000 and leaves heart 2min, take
The supernatant obtains 360mL peach glue colloidal sol.
Take addition 0.072g silver nitrate, room temperature 25 DEG C in above-mentioned peach glue colloidal sol 200mL
Lower stirring, puts into heated and stirred in magnetic force heating stirrer after it dissolves, and temperature sets
Being 100 DEG C, react 3 hours, be cooled to room temperature, and dilute, obtaining concentration is
The golden yellow nano silver colloidal sol of 252mg/L.
In 100mL distilled water, add acetic acid and the 0.25g chitosan of 3mL, treat that it is complete
Add 12g carbamide after CL, add 21.95g zinc acetate, after it is completely dissolved,
Solution is imported in the there-necked flask of 250mL, 80 DEG C be slowly stirred in the case of be incubated 4 little
Time, obtaining the leucosol containing flocculence material, room temperature stands transparent colloidal sol after 12h,
Dilute, obtaining concentration is 4.575 × 103Mg/L nano-zinc oxide sol.
Above-mentioned prepared nano silver colloidal sol and nano-zinc oxide sol 1:2 by volume are mixed
Close, prepare natural polymer/inorganic nano antiseptic composite.
Use natural polymer/nothing that the present embodiment is prepared by the method identical with embodiment
Machine nano-antibacterial composite material is tested, and test result shows: escherichia coli is
Low bacteriocidal concentration is nanometer silver and nano oxidized zinc concentration be respectively 0.7mg/L and
38.125mg/L；The minimum bactericidal concentration of staphylococcus aureus is nanometer silver and nano oxygen
Change zinc concentration and be respectively 0.7mg/L and 38.125mg/L.As it is shown in figure 5, antibacterial survey
Examination MIC effect photo (above arranging escherichia coli, lower row staphylococcus aureus) display:
For escherichia coli, during antimicrobial composite material concentration dilution to 1:64, there is muddiness in solution,
For staphylococcus aureus, during antimicrobial composite material concentration dilution to 1:64, solution occurs
Muddy.
By the above detailed description to the embodiment of the present invention, it will be appreciated that the present invention solves
Conventional method prepares antimicrobial composite material cost height and the problem of complex process, prepares simultaneously
Natural polymer/inorganic nano antiseptic composite good anti-bacterial effect, antibacterial timeliness length and
To nontoxic, it is adaptable to preparation bactericidal liquid, antibacterial etc., it is possible to as antibacterial interpolation
Antibiotic paint is prepared in agent.
It is understood that the principle that embodiment of above is intended to be merely illustrative of the present
And the illustrative embodiments used, but the invention is not limited in this.For ability
For those of ordinary skill in territory, in the situation without departing from spirit and substance of the present invention
Under, various modification and improvement can be made, these modification and improvement are also considered as the present invention's
Protection domain.
Claims (6)
1. natural polymer/inorganic nano antiseptic composite, it is characterised in that: by
Nano silver colloidal sol and nano-zinc oxide sol mix, nanometer in described nano silver colloidal sol
Silver concentration is 80-1.12 × 103mg/L；Zinc oxide concentration in described nano-zinc oxide sol
It is 4 × 103-5×103mg/L；Described nano silver colloidal sol and nano-zinc oxide sol volume ratio
For 1:2-1:3, its preparation method comprises the following steps:
(1) preparation of nano silver colloidal sol: Resina persicae is made peach glue colloidal sol, then to Resina persicae
The hydrosol adds soluble inorganic Ag-containing compound, stirs to soluble inorganic under room temperature
Ag-containing compound is completely dissolved, then containing silver sol, this is heated to 100-120 DEG C, reaction
3-4h, and be cooled to room temperature and obtain nano silver colloidal sol, dilute, obtain silver ion dense
Degree is 80-1.12 × 103Mg/L containing silver sol；
(2) preparation of nano-zinc oxide sol: under room temperature, by 3-3.5 parts by weight acetic acid,
0.2-0.4 weight portion chitosan is dissolved in 100 weight parts waters, adds 11-13 weight portion
Carbamide, adds 20-23 weight portion soluble zinc salt, adds after stirring and dissolving after stirring and dissolving
Heat, to 75-85 DEG C, reacts 3-6h, and dilute, and obtaining zinc oxide concentration is 4 × 103-5
×103The nano-zinc oxide sol of mg/L；
(3) preparation of natural polymer/inorganic nano antiseptic composite: by step (1)
Gained nano silver colloidal sol and step (2) gained nano-zinc oxide sol 1:2-1:3 by volume
Mixing, obtains natural polymer/inorganic nano antiseptic composite.
Natural polymer the most according to claim 1/inorganic nano antiseptic composite wood
The preparation method of material, it is characterised in that comprise the following steps:
(1) preparation of nano silver colloidal sol: Resina persicae is made peach glue colloidal sol, then to Resina persicae
The hydrosol adds soluble inorganic Ag-containing compound, stirs to soluble inorganic under room temperature
Ag-containing compound is completely dissolved, then containing silver sol, this is heated to 100-120 DEG C, reaction
3-4h, and be cooled to room temperature and obtain nano silver colloidal sol, dilute, obtain silver ion dense
Degree is 80-1.12 × 103Mg/L containing silver sol；
(2) preparation of nano-zinc oxide sol: under room temperature, by 3-3.5 parts by weight acetic acid,
0.2-0.4 weight portion chitosan is dissolved in 100 weight parts waters, adds 11-13 weight portion
Carbamide, adds 20-23 weight portion soluble zinc salt, adds after stirring and dissolving after stirring and dissolving
Heat, to 75-85 DEG C, reacts 3-6h, and dilute, and obtaining zinc oxide concentration is 4 × 103-5
×103The nano-zinc oxide sol of mg/L；
(3) preparation of natural polymer/inorganic nano antiseptic composite: by step (1)
Gained nano silver colloidal sol and step (2) gained nano-zinc oxide sol 1:2-1:3 by volume
Mixing, obtains natural polymer/inorganic nano antiseptic composite.
Natural polymer/inorganic nano antiseptic composite the most according to claim 2
Preparation method, it is characterised in that step (1) described peach glue colloidal sol is dissolved in water by Resina persicae,
Remove impurity, and drying, pulverization process, cross the sieve of 250 mesh, obtain peach gum powder
End, then obtain 3 × 10 by soluble in water for Resina persicae powder3-7×103The solution of mg/L, centrifugal
Take the supernatant to obtain.
Natural polymer/inorganic nano antiseptic composite the most according to claim 3
Preparation method, it is characterised in that described be dried be placed in baking oven in 60 DEG C of dry 36h；
Described centrifugal it be placed in centrifuge 3000 and leave heart 2min.
Natural polymer/inorganic nano antiseptic composite the most according to claim 2
Preparation method, it is characterised in that step (1) described soluble inorganic Ag-containing compound bag
Include silver nitrate or silver sulfate.
Natural polymer/inorganic nano antiseptic composite the most according to claim 2
Preparation method, it is characterised in that step (2) described soluble zinc salt is
Zn(CH3COO)2·2H2O or ZnSO4·7H2O。
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| CN102847193B (en) * | 2012-07-04 | 2015-04-29 | 杭州科洛生物技术有限公司 | Chitosan fluid dressing film |
| CN103463667A (en) * | 2013-05-07 | 2013-12-25 | 哈尔滨工程大学 | Preparation method of nanosilver-carried calcium alginate antibiotic medical dressing |
| CN103262859B (en) * | 2013-05-14 | 2015-04-08 | 武汉理工大学 | Preparation method of persimmon tannin-modified nanometer silver composite antibacterial powder |
| CN103586461B (en) * | 2013-11-16 | 2016-01-20 | 华中科技大学 | A kind of nano silver colloidal sol and preparation and purification method thereof |
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2014
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