CN103880926A - Polypeptide, detection device and detection kit comprising same - Google Patents
Polypeptide, detection device and detection kit comprising same Download PDFInfo
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- CN103880926A CN103880926A CN201210560335.XA CN201210560335A CN103880926A CN 103880926 A CN103880926 A CN 103880926A CN 201210560335 A CN201210560335 A CN 201210560335A CN 103880926 A CN103880926 A CN 103880926A
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Abstract
The invention relates to polypeptide, a detection device and a detection kit comprising the same. The polypeptide is composed of an amino acid sequence represented as SEQ ID No.1. The polypeptide, the detection device and the detection kit comprising the same of the present invention are useful in the diagnosis of sicca syndrome.
Description
Technical field
Detection means and detection kit that the present invention relates generally to polypeptide, comprises this polypeptide, belong to biological technical field.
Background technology
Sjogren syndrome (Sjogren Syndrome, SS) is a kind of to invade the exocrine glands such as lachrymal gland, sialisterium as main chronic autoimmune disorder, is called again autoimmunity exocrine gland disease.Main manifestations is keratoconjunctivitis sicca, xerostomia or other rheumatisms such as rheumatoid arthritis that occur together, and it can involve other system and cause multisystem, many organs impaired as breathed system, Digestive, urinary system, blood system, nervous system and muscle, joint etc.
Sjogren syndrome is a global disease, and 90 % are women above, and age of onset is mostly the person in middle and old age of 40-60 years old, and children's is less.Owing to lacking so far unified Case definition, so this sick morbidity is very inaccurate.Generally be estimated as 0.1 %-0.7 %.In the U.S., the sickness rate of sjogren syndrome is only second to rheumatoid arthritis.Also be not less than the sickness rate of rheumatoid arthritis 0.3 %～0.4 % in the morbidity of China.
Diagnosis of xerosis mainly depends on clinical symptom sign performance (the puckery disease of dry, keratitis sicca etc.) and diagnoses.But only judge and be also difficult to and distinguish mutually as other the autoimmune disorder such as connective tissue disease by symptom, therefore also need to detect auxiliary diagnosis by detecting specific autoantibody in serum.
Summary of the invention
Antibody, the detection means that comprises this polypeptide, the detection kit that comprises this polypeptide or this detection means and this polypeptide that the object of the present invention is to provide a kind of nucleic acid, the expression vector that comprises this nucleic acid to the useful polypeptide of the diagnosis of sjogren syndrome, this polypeptide of encoding, has imported the host cell of this expression vector, anti-this polypeptide is detecting purposes in sjogren syndrome, this polypeptide in the purposes for the preparation of detecting in test kit or the detection means of sjogren syndrome., the present invention comprises following technical proposals:
1. the polypeptide being formed by the aminoacid sequence shown in SEQ ID NO:1.
2. the nucleic acid of coding polypeptide claimed in claim 1.
3. comprise the expression vector of nucleic acid claimed in claim 2.
4. imported the host cell of expression vector claimed in claim 3.
5. a detection means, it comprises:
Solid carrier, and
Be connected in the polypeptide claimed in claim 1 on this solid carrier.
6. detection means according to claim 5, wherein, described solid carrier is SJ modified silica-gel.
7. a detection kit, it comprises the detection means described in polypeptide claimed in claim 1 or claim 5 or 6.
8. the antibody of anti-polypeptide claimed in claim 1.
9. the purposes of polypeptide claimed in claim 1 in test kit or detection means for the preparation of detection sjogren syndrome.
Polypeptide of the present invention is applied to the diagnosis of sjogren syndrome, can obtains gratifying effect.
Accompanying drawing explanation
Fig. 1 is fixed on polypeptide the schematic diagram on SJ modified silica-gel surface by chemical covalency.
The HPLC collection of illustrative plates that Fig. 2 confirms the polypeptide of the present invention of chemosynthesis.
Fig. 3 is that the MS that the polypeptide of the present invention of chemosynthesis is confirmed characterizes collection of illustrative plates.The schematic diagram that Fig. 4 describes the making processes of SJ modified silica-gel (iPDMS film).
The figure that Fig. 5 describes polypeptide microarray chemistry fixation procedure.
Fig. 6 illustrates the schematic diagram of polypeptide microarray spot sample mode.
Fig. 7 shows the result photo that healthy normal people or non-sjogren syndrome patients serum are detected.
Fig. 8 shows the result photo that sjogren syndrome patients serum is detected.
Embodiment
polypeptide of the present invention
Polypeptide of the present invention is 20 peptides.20 peptides of the present invention are made up of the aminoacid sequence shown in SEQ ID NO:1, that is: QCSDISTKQAAFKAVSEVCR.As shown in the Examples, its serum to sjogren syndrome patient is positive, and to healthy normal people or the non-sjogren syndrome patients serum reaction that is negative.Therefore, this 20 peptide is useful as the diagnostic tool of sjogren syndrome.
Polypeptide of the present invention, having commercially available in the situation that, can use commercially available product, in addition, can also suitable employing (1) chemical synthesis process or the known method such as (2) enzyme reaction synthetic method obtain, wherein chemosynthesis is more easy.In chemosynthesis polypeptide situation of the present invention, undertaken by or semi-synthetic this polypeptide synthetic with peptide synthesizer.As chemical synthesis process, can list such as peptide solid-phase synthesis etc.Synthetic like this peptide can adopt conventional means such as ion-exchange chromatography, reversed phase high efficiency liquid color spectrum, affinity chromatography etc. to carry out purifying.Such peptide solid phase synthesis process with and subsequent peptide purification be all well-known in the art.
In addition; in the situation that producing polypeptide of the present invention by enzyme reaction; can adopt the method described in No. WO2004/011653, International Publication brochure for example: by esterified the C-terminal of a side amino acid or dipeptides or amidation and the amino acid obtaining or dipeptides, amino acid (amino acid of for example carboxy protective) with amino acid in unbound state under the existence of peptide synthetase, react, the dipeptides of generation or tripeptides.As peptide synthetase, can list: bacterial disposing thing or this microbe-derived peptide synthetase with culture, the microbial cells being separated by this culture or this microorganism of the microorganism of the ability that generates peptide.
And except above-mentioned enzyme method, chemical synthesis process, in some cases, polypeptide of the present invention may be also natural existence (but not being separated).In naturally occurring situation, can also be separated.
nucleic acid of the present invention, expression vector host cell, and the antibody of anti-polypeptide of the present invention
The invention still further relates to the nucleic acid (nucleic acid of the present invention), the expression vector (expression vector of the present invention) that comprises this nucleic acid of this polypeptide of coding, the host cell (host cell of the present invention) that has imported this expression vector, they preferably can be used for producing polypeptide of the present invention.Nucleic acid of the present invention, expression vector, host cell can adopt the method for well known to a person skilled in the art to prepare.The antibody that the invention still further relates to anti-polypeptide of the present invention, it can be used for detecting antibody of the present invention.Antibody of the present invention can adopt the method for well known to a person skilled in the art to prepare.
detection means of the present invention
The invention still further relates to a kind of detection means (detection means of the present invention), it comprises solid carrier and is connected in the polypeptide of the present invention on this solid carrier.
In the present invention, solid carrier is not particularly limited, as long as for example, carrier as solid or insoluble material (being the material that can separate from reaction mixture by filtration, precipitation, magnetic resolution etc.).
The material that forms solid carrier includes but not limited to: silica gel (polydimethylsiloxane, PDMS), Mierocrystalline cellulose, teflon
tM, Nitrocellulose, agarose, dextran, chitosan, polystyrene, polyacrylamide, polyester, polycarbonate, polymeric amide, polypropylene, nylon, poly(vinylidene fluoride), latex, silicon-dioxide, glass, glass fibre, gold, platinum, silver, copper, iron, stainless steel, ferrite, silicon wafer, polyethylene, polymine, poly(lactic acid), resin, polyose, albumen (albumin etc.), carbon or their combination etc.
The shape of solid carrier comprises but is not limited to: pearl, magnetic bead, film, microcapillary, filter membrane, plate, micro plate, carbon nanotube, sensor chip etc.Pit, groove, filter membrane bottom etc. can be set on the solid carrier that just as known in the art, film or plate etc. are smooth.
In the present invention, magnetic bead can have the sphere diameter of about 25nm ~ about 1mm scope.In a preferred embodiment, magnetic bead has the diameter of about 50nm ~ approximately 10 μ m scope.The size of magnetic bead can be selected according to specific purposes.
In the present invention, the pearl of being made up of the contour crosslinked spherical agarose of Sepharose has the diameter of approximately 24 μ m ~ approximately 165 μ m scope.Preferably, high crosslinked spherical sepharose 4B has the diameter of approximately 24 μ m ~ approximately 44 μ m scope.The size of high crosslinked spherical sepharose 4B can be selected according to specific purposes.
The example with the solid carrier of water repellent surface comprises can be from Polysciences, Warrington, PA or Spherotech, Liberville, the polystyrene latex beads such as the goods that IL buys.
Silicon-dioxide (SiO
2)-process or silicon-dioxide (SiO
2) example of solid carrier of base comprises can be from Polysciences, Warrington, the extraordinary magnetic silica pearl that PA buys etc., it can for example, for catching nucleic acid (DNA).Or, can also use the M-280 etc. that can buy from Dynal Biotech.
The magnetic bead with hydrophilic surface can be used for catching bacterial cell, nucleic acid and other composition of proliferation period.As the example of this magnetic bead, can list Polysciences, Warrington, pearl (title: Biomag (registered trademark) carboxyl) or Bangs Laboratory that PA sells, Inc., Fishers, the name of IN is called the pearl of MC02N/2928.Or, can use the M-270 of Dynal Biotech sale etc.
In a preferred embodiment of the present invention, described solid carrier is SJ modified silica-gel, for the microarray solid support material (having another name called iPDMS film, referring to Chinese patent CN101265329A) of a kind of silicon rubber material of Suzhou Siju Biomaterials Co., Ltd. exploitation.This material is take the conventional PDMS of biological study as basis, add therein specific initiator composition (making this material realize surface-functionalized modification by surface initiated polymerization (SIP)), pass through again polyethylene glycol methacrylate-styrene polymer (poly (oligo (ethylene glycol) methacrylate), pOEGMA) finishing obtain.SJ modified silica-gel has outstanding anti-protein non-specific adsorption (Nonspecific protein adsorption, NPA) ability, non-specific protein absorption in complicated protein immunization can being detected controls to " absolute 0 " level (being near or below the limit of detection of instrument) that approaches, the trouble that not only can exempt sealing and repeatedly clean, can also be by improving the susceptibility of protein microarray by stronger amplification of signal means.And the essence of its silicon rubber has been given mechanical property that this material is stronger and good operability.The poly-combination assay microarray ELISA test kit that successfully SJ modified silica-gel has been applied to 11 tumor markers compositions of Suzhou Yvonne, realize high-throughput and high-sensitive detection, proved that this material is a kind of outstanding protein microarray solid support material.Meanwhile, this material also has the adjustable characteristic of surface properties, can adjust within the specific limits its surface topography by the controlled modification reaction times.
Polypeptide of the present invention can adopt the method for attachment that well known to a person skilled in the art polypeptide and solid carrier to carry out with being connected of solid carrier.For example, for being connected of protein/polypeptide and modified silica-gel surface, can pass through 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide [1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide, EDC] and N-hydroxy-succinamide (N-hydroxysuccinimide, NHS) reaction changes carboxyl (COOH) group on the macromolecular chain of modified silica-gel surface into activating group, this activating group can with on protein/polypeptide with amino (NH2) thus react realize protein/polypeptide is fixed on to solid carrier surface (referring to Fig. 1).
In the sampling liquid using during for point sample, the concentration of polypeptide of the present invention is not particularly limited, and those skilled in the art can select according to conventional, are preferably 1 μ g ~ 1000 μ g/mL, more preferably 10 μ g ~ 500 μ g/mL.In addition, the density distributing on solid carrier for polypeptide of the present invention is not particularly limited, and those skilled in the art can select according to conventional, are preferably 1 ~ 100 points/10mm2, more preferably 5 ~ 50 points/10mm2.
Detection means of the present invention can be for detection of sjogren syndrome or for the preparation of the test kit that detects sjogren syndrome.
detection kit of the present invention
The invention still further relates to a kind of detection kit (detection kit of the present invention), it comprises polypeptide of the present invention or detection means.This detection kit is preferred for detecting sjogren syndrome.
Detection means of the present invention or polypeptide of the present invention are the important documents of detection kit of the present invention.Detection kit of the present invention can also comprise:
1. the serum dilution preparing or serum dilution component solution: serum dilution, for example, have sample diluent (production code member 070021-S2), the application of sample variable color sample diluent (production code member bwj010103) of Bo Weijia bio tech ltd, Zhengzhou etc. of Sai Chi bio tech ltd, Beijing.This serum dilution is used for dilute serum, and the serum that test kit detects will dilute suitable multiple, and for example 2 ~ 200 times, preferably 10 ~ 100 times.
Detection kit of the present invention can also comprise:
2. concentrated washing lotion: solid carrier surface is hatched after serum and ELIAS secondary antibody, need wash the unconjugated antibody of solid carrier surface and ELIAS secondary antibody by washing lotion.Concentrated washing lotion is for example 1% the polysorbas20 aqueous solution, need dilute 2 ~ 40 times, preferably 5 ~ 20 times when use.
Detection kit of the present invention can also comprise:
3. ELIAS secondary antibody solution: the sjogren syndrome autoantibody (IgG) in sjogren syndrome patients serum can be combined by for example, polypeptide of the present invention on solid carrier (SJ modified silica-gel), two anti-can with antibodies, and two markers that resist can react with luminous substrate, thereby send detectable light.ELIAS secondary antibody can be the goat anti-human igg of for example horseradish peroxidase-labeled.As ELIAS secondary antibody solution, can list the mountain goat anti-human igg (H+L) of the horseradish peroxidase-labeled of Beijing Bioisystech Co., Ltd of Zhong Shan Golden Bridge production, production code member ZB-2304.Concentration to ELIAS secondary antibody in ELIAS secondary antibody solution is not particularly limited, and can be for example 1ng ~ 1000ng/mL.
Detection kit of the present invention can also comprise:
4. luminescent solution component solution: luminescent solution can react with the horseradish peroxidase of two anti-upper marks, makes reaction send the chemical light that instrument can detect.Luminescent solution is mixed by two kinds of solution, is respectively A liquid-superoxol, and B liquid-luminol solution.Luminol (luminol,3-aminophthalic acid cyclic hydrazide) only has that cross just by oxidizer treatment can be luminous.Conventionally use the mixed aqueous solution of hydrogen peroxide and a kind of hydroxide bases as exciting agent.Under horseradish peroxidase enzyme catalytic, decomposing hydrogen dioxide solution is oxygen G&W:
2 H
2O
2→ O
2 + 2 H
2O
Luminol,3-aminophthalic acid cyclic hydrazide has generated a pairs of anion while reaction with oxyhydroxide, the dioxygen oxidation that it can be decomposited by hydrogen peroxide, and product is an organo-peroxide.This superoxide is very unstable, decomposites immediately nitrogen, generates the 3-aminophthalic acid of excited state.During excited state to ground state transforms, the energy of release exists with the form of photon, and wavelength is positioned at the blue light part of visible ray.The SuperSignal ELISA Femto Maximum Sensitivity Substrate of for example Thermo Seientific company of the example of luminescent solution component solution, article No. 37074.
Detection kit of the present invention can also comprise:
5. one or more reaction cavity (for example Chinese patent Granted publication CN202054829U).
Detection kit of the present invention can also comprise:
6. other detection molecules for detection of sjogren syndrome (such as polypeptide, protein, nucleic acid etc.).
Detection kit of the present invention can also comprise:
7. working instructions.
Embodiment
Below, by embodiment, the present invention is carried out to more specific description, but be not the restriction to the technology of the present invention scope.By the record of this specification sheets, those skilled in the art can be easy to the present invention to modify/change, and these are included in technical scope of the present invention.
the preparation of 1.30 peptides and confirmation
20 peptides that use in embodiment have the aminoacid sequence shown in SEQ ID NO:1, and by gill, biochemical (Shanghai) Co., Ltd. is synthetic, and the sign collection of illustrative plates of this polypeptide is shown in Fig. 2 and Fig. 3, can confirm to have synthesized described polypeptide.
2. the preparation of detection means
Detection chip is take SJ modified silica-gel (iPDMS film) as solid support material, is prepared from thereon by point sample immobilized polypeptide solution.Modified silica-gel is in traditional polydimethylsiloxane material, to add with initiator olefin-terminal, surface initiated polymerization, and be fixed in the three-dimensional structure of polydimethylsiloxane by heat cross-linking (si-h bond bonding), obtaining a kind of new material is SJ modified silica-gel.Its making processes as shown in Figure 4.
A wherein and B are two components of polydimethylsiloxane, polydimethylsiloxane (Poly (dimethylsiloxane), Sylgard 184) buy from Dow corning (DowCorning) company, comprising liquid composition A(composition is metal platinum catalyzer and the diformazan siloxanes polymer precursor mixture with vinyl) and two kinds of compositions of crosslinking agent B (composition is the dimethyl siloxane precursor with vinyl and Si-H group).C is the initiator of end band vinyl, is purchased from Hangzhou Dong Wei company.Polymer on finally modifying is that oligomeric ethylene glycol methacrylate monomer (Oligo (ethylene glycol) methacrylate, hereinafter to be referred as OEGMA, molecular weight Mw=526) is bought in Aldrich.Polydimethylsiloxane precursor A and crosslinking agent B are fully mixed with A:B:C=10:1:0.5 ratio with the initiator C with vinyl end.Make transparent elastic silicone rubber by curing reaction, then carry out finishing by SIP technology and can obtain SJ modified silica-gel.Experiment shows, the surface of SJ modified silica-gel have enough highdensity, by the fixing initiator of covalent linkage, it can pass through surface initiated polymerization (SIP), and to realize surface macromolecule modified.Use poly (OEGMA) (polyethylene glycol methacrylate-styrene polymer) to react and obtain the surface that polyoxyethylene glycol (Polyethylene Glycol, PEG) is modified, realize the ability of stronger anti-albumen non-specific adsorption.
The SJ modified silica-gel film making need be kept in 4 ℃ of refrigerators.
Adopt 16 people's point sample instruments of brilliant core PersonalArrayerTM on modified silica-gel, to prepare polypeptide microarray, process is:
1) pre-treatment
By SJ modified silica-gel thin slice (15 × 15mm
2) be immersed in activation solution, after 30min, take out with deionized water drip washing 3 times, dry up with nitrogen, at once for point sample.
2) point sample
Sampling liquid is diluted and gets well and transfer in the corresponding micropore of 384 orifice plate, will be placed on point sample instrument base station with 384 orifice plates of sample, pretreated modified silica-gel thin slice is placed on the base station of point sample instrument simultaneously, carry out point sample at once.Point sample envrionment conditions is room temperature (25 ℃), and humidity is set as 50%.On the polypeptide microarray of making, the point sample amount of each point is about 0.6nL, and sampling point radius is 200 μ m.
3) chemistry is fixing
The polypeptide microarray just having made will be placed on fixing at least 6h in climatic chamber (26 ℃, 60% humidity).Chemistry fixation procedure as shown in Figure 5.
First will include the damping fluid point of catching peptide molecule on modified silica-gel film by point sample instrument, then damping fluid start vaporizer, catch peptide molecule and the surface intimate contact of SJ modified silica-gel and interact, by Chemical bond, the high molecular end-COOH of the ploy on modified silica-gel surface (OEGMA) and peptide molecule--NH
2form and stablize covalent linkage, and then will have chemically active peptide molecule to be fixed on SJ modified silica-gel surface.
5) assembling
The polypeptide microarray of fixing 6h must assemble in two days.First by gum, SJ modified silica-gel thin slice is attached on special reaction column, covers reaction cavity.A reactor is made up of two reaction columns and a reaction cavity.
6) preserve
The polypeptide microarray assembling, need to vacuumize sealing, is kept in the refrigerator of 4 ℃, for subsequent use.
3. detect by detection means
Checking procedure
1, before starting to detect, add purified water or distilled water to dilute in the ratio of 1:10 concentrated cleaning solutions, diluted rear direct use.Use liquid-transfering gun that 2mL scavenging solution is added to chip surface, soak chip 3 minutes, guarantee that chip surface is by complete wetting.
2, test serum sample is mixed according to 1:40 dilution with sample diluent.
3, discard the scavenging solution that soaks chip, under the completely moistening state of chip surface, the serum that each serum sample is drawn after 200 μ L dilutions joins in chip reactor.
4, chip reactor is put into chip permanent seat, be put on shaking table, open shaking table, 150 revs/min of frequencies, incubated at room 30 minutes.
5, discard the serum sample in chip reactor, use 15mL washing lotion cleaning reaction cavity and chip surface 3 times.
6, after having cleaned, each chip reactor adds respectively 200 μ L enzyme labelled antibody solution, and chip reactor is put into chip permanent seat, is put on shaking table, opens shaking table, 150 revs/min of frequencies, incubated at room 30 minutes.
7, discard the enzyme labelled antibody solution in chip reactor, use 15mL washing lotion cleaning reaction cavity and chip surface 3 times.
8, after having cleaned, take off reaction cavity, each chip surface adds respectively 15 μ L luminous substrate liquid, makes luminescent solution can be laid on uniformly chip surface.
9, the chip that has added luminescent solution is placed in to gel imaging instrument chemoluminescence imaging, and sentence read result.
Sjogren syndrome patients serum derives from Shenyang No. 1 Hospital Affiliated to Chinese Medical Univ, be clinical definite the sick patients serum of sjogren syndrome.Serum is transported or is handed to soon laboratory with parcels such as ice cube/dry ice by related personnel.
Negative control has the contrast of PBS damping fluid (hatch without test serum in the 3rd step, and hatch with PBS solution, all the other steps are identical), the contrast of serum dilution, and the contrast of patients with negative (referring to Healthy Human Serum and non-sjogren syndrome patient) serum.
The spot sample mode of polypeptide microarray as shown in Figure 6.Wherein, the sample of leg-of-mutton 20 points is human IgG, as the locating point of experiment; The sample of foursquare 4 points is PB sampling liquid, as the blank of experiment; Circular point sample be other sjogren syndrome autoantigen protein polypeptide, as the detection index (these polypeptide have response explanation to detect in serum and have sjogren syndrome autoantibody) of experiment; The sample polypeptide of star point is polypeptide SEQ ID NO:1 of the present invention, and it is sjogren syndrome autoantigen protein polypeptide, can produce response to Patients with Sjogren Syndrome serum.
Experimental result as shown in Figure 7 and Figure 8.Wherein, Fig. 7 has shown the detected result of negative control, only has the sample of the point shown in trilateral to have response.Fig. 8 has shown sjogren syndrome patients serum's detected result, and the sample of trilateral, circle and star point has response.It should be noted that, instrument records signal value from low to high, and corresponding signaling point color is by black-red-white gradual change.
<110> Suzhou Siju Biomaterials Co., Ltd.
<120> polypeptide, the detection means that comprises this polypeptide and detection kit
<130> Ro60-11
<160> 1
<170> PatentIn version 3.1
<210> 1
<211> 20
<212> PRT
<213> artificial sequence
<220>
<223> sjogren syndrome antigenic polypeptide
<400> 1
QCSDISTKQAAFKAVSEVCR 20
Claims (9)
1. the polypeptide being formed by the aminoacid sequence shown in SEQ ID NO:1.
2. the nucleic acid of coding polypeptide claimed in claim 1.
3. comprise the expression vector of nucleic acid claimed in claim 2.
4. imported the host cell of expression vector claimed in claim 3.
5. a detection means, it comprises:
Solid carrier, and
Be connected in the polypeptide claimed in claim 1 on this solid carrier.
6. detection means according to claim 5, wherein, described solid carrier is SJ modified silica-gel.
7. a detection kit, it comprises the detection means described in polypeptide claimed in claim 1 or claim 5 or 6.
8. the antibody of anti-polypeptide claimed in claim 1.
9. the purposes of polypeptide claimed in claim 1 in test kit or detection means for the preparation of detection sjogren syndrome.
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|---|---|---|---|
| CN201210560335.XA CN103880926A (en) | 2012-12-21 | 2012-12-21 | Polypeptide, detection device and detection kit comprising same |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210560335.XA CN103880926A (en) | 2012-12-21 | 2012-12-21 | Polypeptide, detection device and detection kit comprising same |
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| Publication Number | Publication Date |
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| CN103880926A true CN103880926A (en) | 2014-06-25 |
Family
ID=50950087
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| CN201210560335.XA Pending CN103880926A (en) | 2012-12-21 | 2012-12-21 | Polypeptide, detection device and detection kit comprising same |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105753943A (en) * | 2016-02-18 | 2016-07-13 | 苏州偲聚生物材料有限公司 | Detecting device, detection kit and diagnosis method for diagnosing systemic lupus erythematosus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040078035A1 (en) * | 1999-01-25 | 2004-04-22 | Olympus Optical Co., Ltd. | Medical treatment instrument |
| CN1935837A (en) * | 2006-08-30 | 2007-03-28 | 中国科学院广州生物医药与健康研究院 | Beta-fodrin antigen epitope polypeptide, and its screening method and use |
-
2012
- 2012-12-21 CN CN201210560335.XA patent/CN103880926A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040078035A1 (en) * | 1999-01-25 | 2004-04-22 | Olympus Optical Co., Ltd. | Medical treatment instrument |
| CN1935837A (en) * | 2006-08-30 | 2007-03-28 | 中国科学院广州生物医药与健康研究院 | Beta-fodrin antigen epitope polypeptide, and its screening method and use |
Non-Patent Citations (3)
| Title |
|---|
| ROUTSIAS JG ET AL.: "Sjogren’s Syndrome-Study of Autoantigens", 《CLINIC REV ALLERG IMMUNOL》 * |
| TAWATCHAI PAISANSINSUP ET AL.: "HLA Class II Influences the Immune Response and Antibody Diversification to Ro60/Sjo gren’s Syndrome-A: Heightened Antibody Responses and Epitope Spreading in Mice Expressing HLA-DR molecules", 《THE JOURNAL OF IMMUNOLOGY》 * |
| TAWATCHAI PAISANSINSUP ET AL.: "HLA Class II Influences the Immune Response and Antibody Diversification to Ro60/Sjo gren’s Syndrome-A: Heightened Antibody Responses and Epitope Spreading in Mice Expressing HLA-DR molecules", 《THE JOURNAL OF IMMUNOLOGY》, vol. 168, no. 11, 1 June 2002 (2002-06-01) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105753943A (en) * | 2016-02-18 | 2016-07-13 | 苏州偲聚生物材料有限公司 | Detecting device, detection kit and diagnosis method for diagnosing systemic lupus erythematosus |
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Application publication date: 20140625 |