CN103877446A - Wheat fiber powder preparation and preparation method and use thereof - Google Patents
Wheat fiber powder preparation and preparation method and use thereof Download PDFInfo
- Publication number
- CN103877446A CN103877446A CN201410138907.4A CN201410138907A CN103877446A CN 103877446 A CN103877446 A CN 103877446A CN 201410138907 A CN201410138907 A CN 201410138907A CN 103877446 A CN103877446 A CN 103877446A
- Authority
- CN
- China
- Prior art keywords
- wheat
- preparation
- fructus hordei
- hordei germinatus
- powder preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of traditional Chinese medicines, and particularly relates to a wheat fiber powder preparation used for treating ulcerative colitis and irritable bowel syndrome, and a preparation method and use thereof. The wheat fiber powder preparation includes the following raw material medicines in parts by weight: 4-7 parts of malt fiber, 4-5 parts of Poria cocos and 4-5 parts of Chinese yam. The invention further provides a preparation method and use of the wheat fiber powder preparation. Wheat fiber powder has an exact curative effect of treating the ulcerative colitis and the irritable bowel syndrome. The wheat fiber powder preparation has good effects of regulating intestinal bacteria, treating the irritable bowel syndrome and the ulcerative colitis, and the like. Medicinal materials used by the wheat fiber powder preparation provided by the invention are usually long-term edible medicine and food dual-purpose materials, so that the wheat fiber powder preparation is low in cost and free of any toxic or side effect, and has a very obvious effect of perfecting the intestinal bacteria.
Description
Technical field
The present invention relates to the field of Chinese medicines, in particular for the fine powder preparation of wheat for the treatment of ulcerative colitis and irritable bowel syndrome, and its production and use.
Background technology
Ulcerative colitis (ulcerative colitis, UC) is a kind of agnogenic rectum and colon chronic nonspecific inflammation enteropathy, is one of digestive system commonly encountered diseases.This disease sickness rate and prevalence are significantly increased trend in China in recent years.Suffer from the more than 10 years person of primary disease, the incidence rate of concurrent colon cancer is in 1% left and right.Because the touching difficulty of primary disease heals, lesion degree weight is different, and relapse rate is high, has been listed in one of modern difficult treatment.Research both at home and abroad all the time generally believes that it is relevant with the factor such as heredity, immunity, infection, environment, diet, spirit, modern medicine to the treatment of UC except colectomy, there is no specific short, general opinion internal medicine treatment is main, main medicine has following a few class: aminosalicylic acids is as sulfasalazine, mesalazine etc., cortical steroid is as prednisone, dexamethasone, hydrocortisone etc., and immunosuppressant class is as azathioprine and 6-MP, methotrexate etc.The upper doctor trained in Western medicine of at present treatment is still using aminosalicylic acids and hormone medicine as choice drug, although these medicines have rapid-action short-term advantage, but life-time service side effect is large, even the novel bigcatkin willow acid supplement that the side effect of new listing is relatively less, also eventually because of expensive, after drug withdrawal, easily recur and the shortcomings such as fulminant type and part intractable patient weak curative effect have been subject to challenge.Therefore, seek more efficientlyly, and few side effects, the ideal medicament that is difficult for recurrence have become the focus of research.Along with microbiological development, research finds that the intestinal microbial population of UC patient and animal model is in disturbance state, recover intestinal microbial population to remission have the reduction of a series of disease activity indexs after normal condition, therefore intestinal microbial population state is more and more subject to people's attention, and corresponding microbial ecological agent also arises at the historic moment.In addition, research shows UC when morbidity, exists short-chain fatty acid (short-chainfattyacids, SCFA) synthetic not enough or utilize the situation of obstacle, supplementary SCFA is conducive to the reparation of ulcer and the improvement of symptom, therefore the effect of SCFA also can not be ignored in ulcerative colitis research.Chinese medicine UC has multipath, multi-level, many target spots and few side effects, can long term maintenance the feature for the treatment of and day by day noticeable, therefore the effective formula and drug of further investigation treatment UC has very profound significance.
Irritable bowel syndrome is divided into diarrhea type irritable bowel syndrome (IBS-D), constipation-predominant of irritable bowel syndrome (IBS-C) and alternating diarrhea and constipation type irritable bowel syndrome according to clinical manifestation.The type occurred frequently that wherein IBS-D is irritable bowel syndrome, the demonstration of epidemiological study in recent years, morbidity and the enteric flora disturbance of IBS-D are contacted directly, and the main cause that causes enteric flora disturbance is intestinal infection, a large amount of abuses of antibiotics, feed fiber is very few relevant.Doctor trained in Western medicine often has medicine to be divided at present:
1) spasmolytic: have anticholinergic agents; Selectivity intestinal smooth calcium-ion channel antagonists: this is first medicine for the treatment of IBS for pinaverium bromide (dicetel); Ion channel modulators: Trimebutine Maleate, to improve clinical symptoms, but curative effect is dissatisfied;
2) diarrhea: dioctahedral smectite, clinical commonly used drug, can absorb moisture and pathogenic bacterium, short cell migration; The symptoms such as loperamide, acts on the opiate receptor of intestinal wall, stops acetylcholine and prostaglandin to discharge, and can improve stomachache, and abdomen is anxious;
3) visceral analgesics: mainly project spinal cord afferent inputs nerve and lower internal organs perception by rectum always, can delay the transhipment of mouth-caecum for IBS-D patient;
4) antidepressants: 5-hydroxy tryptamine (5-HT) reuptake inhibitor is as paroxetine, but onset is slow;
Microbial ecological agent, utilizes the normal microorganism useful to host and metabolite thereof and (or) promotes the preparation that the material of its growth and breeding is made, as probiotic bacteria, and prebiotics and symphysis unit etc.Above medicine is for improving IBS typical case diarrhoea, and the symptoms such as stomachache have certain effect.
But Western medicine easily stimulates stomach discomfort, and some drugs toxic side effect.
Summary of the invention
For overcoming above problem, the invention provides a kind of successful, and without any the fine powder preparation of wheat of the medicine-food two-purpose of toxic and side effects.
The fine powder preparation of a kind of wheat, the crude drug that comprises following portions by weight: Fructus Hordei Germinatus fiber 4-7 part, Poria 4-5 part, Rhizoma Dioscoreae 4-5 part.
Preferably, the crude drug that comprises following portions by weight: 6 parts of Fructus Hordei Germinatus fibers, 4 parts, Poria, 4 parts of Rhizoma Dioscoreaes.
Preferably, formed by the crude drug of following portions by weight: Fructus Hordei Germinatus fiber 4-7 part, Poria 4-5 part, Rhizoma Dioscoreae 4-5 part, Herba Portulacae 2-3 part, Radix Notoginseng powder 2-3 part.
Preferred, formed by the crude drug of following portions by weight: 6 parts of Fructus Hordei Germinatus fibers, 4 parts, Poria, 4 parts of Rhizoma Dioscoreaes, 3 parts of Herba Portulacaes, 3 parts of Radix Notoginseng powder.
In addition, the present invention also provides the preparation method and its usage of the fine powder preparation of wheat.
The preparation method of the fine powder preparation of wheat, comprises the steps:
(1), by germinating barley saccharifying processing
Choose high-quality germinating barley, go Mai Gen and selected after, at 47 ℃, adopt dry pulverization process 30min, 90 ℃ are dried to constant weight, the Fructus Hordei Germinatus after being pulverized.In brew kettle, first add suitable quantity of water (Fructus Hordei Germinatus and water quality ratio=1:4), be heated to 37 ℃, then add the Fructus Hordei Germinatus crushing, maintain (65-72 ℃) and manufacture wheat wine with dregs.The climb procedure of wheat wine with dregs temperature adopts secondary to cook method.When saccharifying finishes within the shortest time by wheat juice and wheat groove isolated by filtration.
(2), the extraction of Fructus Hordei Germinatus fiber
After wheat grain adopts indirect type heat drying method to dry, flattening, fragmentation, sieve through 40 mesh standard sieves, adopt alkali-enzyme combined techniques to extract Fructus Hordei Germinatus fiber in wheat groove.
(3), the preparation of the fine powder preparation of wheat
The prepared slices of Chinese crude drugs such as selected Poria, Rhizoma Dioscoreae, Herba Portulacae, Radix Notoginseng, remove respectively impurity, dry, and after pulverizing, then sieve for depositing through 120 mesh standard sieves with Chinese medicine high speed disintegrator, add in Fructus Hordei Germinatus fiber according to proportioning, have fully mixed the fine loose preparation of wheat.
Preferably, in step (1), the climb procedure of wheat wine with dregs temperature adopts secondary to cook method.
The fine powder preparation of wheat is for the preparation of the purposes of improving intestinal microbial population medicine.
The fine powder preparation of wheat is for the preparation of the purposes that prevents and/or treats ulcerative colitis medicine.
The fine powder preparation of wheat is for the preparation of the purposes that prevents and/or treats irritable bowel syndrome medicine.
Preferably, described irritable bowel syndrome is diarrhea type irritable bowel syndrome.
The dosage form of the fine powder preparation of wheat is granule, capsule or tablet.
Wherein, Fructus Hordei Germinatus fiber, Fructus Hordei Germinatus be the mature fruit of standing grain section plant Fructus Hordei Vulgaris through the dry product that germinate, tool promote qi circulation digestion promoting, spleen benefiting and stimulating the appetite, move back the merit of newborn relieving distension, be one of common drug of gastroenteropathy." book on Chinese herbal medicine converge with speech " said: " Fructus Hordei Germinatus, and in the medicine that helps digestion also; Mend and can profit, profit and can mend." " Records of Tradition Chinese and Western Medicine in Combination " also cloud: " can enter taste, digest all diet and gather, be the product of assisting a ruler in governing a country of subsidy taste ... the kind digestion of its property, holds concurrently energy tonneau two just." the fine loose wherein main component of wheat is dietary fiber, its source is during for beer fermentation, inner various enzymes and part disactivation enzyme activation and the growth forming, and the glutamine content in protein increases, and improves the hemicellulose level that forms cell wall.Fructus Hordei Germinatus is extracting after water solublity maltose and protein, the insoluble paddy skin staying, cortex and part plumule, remove and be the complex GBF(Germinated Barley Foodstuff that in Fructus Hordei Germinatus, dietary fiber and protein form after paddy skin), claim again Fructus Hordei Germinatus fiber, wherein contained beta glucan had both had antiinflammatory action, it can reduce again intestinal PH with the short fatty acid (SCFA) that connects of dead end product that large enteral bacterial fermentation produces acquisition simultaneously, promote propagation and the growth of enteral probiotics bacillus bifidus and other probiotics, suppress the growth of enteral Salmonella and putrefaction bacteria, prevent intestinal mucosa atrophy, regulate gastrointestinal function, maintain intestinal microbial balance with healthy, and it is synthetic to increase vitamin, improve immune function of human body.Meanwhile, cellulose, hemicellulose can promote intestinal movement, significantly improve symptom in intestinal, promote intestinal epithelial cell differentiation, promote intestinal mucosa reparation.In addition, in Fructus Hordei Germinatus fibre structure, there are a lot of hydrophilic genes, have very strong water absorption, high-moisture-retention, high-expansion, can promote colon to absorb liquid.Fiber surface has a lot of active genes, also can adsorb the organic molecule such as cholesterol, bile acid, suppress total cholesterol concentration and raise, reduce the synthetic of people's cholesterol and salt and absorb, reduce the cholesterol in human serum and liver, thereby can prevent coronary atherosclerosis and angiocardiopathy preventing.
Poria, property is flat, and slightly sweet flavor can promoting diuresis to eliminate damp pathogen, also can spleen-benefiting mind-tranquilizing, be especially good at treating insufficiency of the spleen wet card very, " sweet with supporing yang, the panacea of dehumidifying is also for Poria, the sharp key of light energy.The flat YANG invigorating of sweet in the mouth, strengthening the spleen is relieved oedema or abdominal distension through diuresis or purgation, the inducing QI of promoting the production of body fluid." (" medication heart method "); " Poria, can dry by sharp key, and sharp key is happy Fructus Alpiniae Oxyphyllae, leads turbid promoting the production of body fluid; The dry spleen of relieving oedema or abdominal distension through diuresis or purgation that dries, invigorating middle warmer stomach invigorating "; " diuretic dehumidifying key medicine the most.Book is said spleen invigorating, water go and spleen from strong meaning also." " book on Chinese herbal medicine is looked for the truth "); modern study proves that it can promote health immunocompetence; be the key medicine of the multiple compatibility of medicines in a prescription and the important source material of Chinese patent medicine, proves that it can move by regulating intestinal canal, all has inhibitory action to staphylococcus aureus, escherichia coli, Bacillus proteus etc. in intestinal simultaneously.
Rhizoma Dioscoreae, sweet in the mouth, property is flat, returns spleen, liver, kidney; Can supplementing QI and nourishing YIN, tonifying the lung strengthening the spleen, treating spontaneous emission and leukorrhagia, is referred to as in Compendium of Material Medica: " kidney tonifying gas, strengthening the spleen and stomach, antidiarrheal dysentery, the saliva that reduces phlegm, profit fur " is the flat product of mending three warmers gas the moon." because of its sweet in the mouth gas perfume (or spice), help spleen with it, control insufficiency of the spleen diarrhoea, tiredness with somnolence, extremity are sleepy.Get again its sweet YANG invigorating, with energy invigorating the spleen and replenishing QI, replenishing vital QI with drugs of warm nature muscle, is the dirty key medicine of lung spleen two." (" medicine justice ").Modern pharmacological research proves that it contains the material such as amylase, polyphenol oxidase, can move in stimulating gastrointestinal road, promote that gastrointestinal contents is emptying, be conducive to taste digestive and absorptive functions, for the weakness of the spleen and stomach better effect of having had loose bowels, there is the intestinal tube smooth muscle spasm of alleviation simultaneously and resist neurohumoral effect, can also strengthen Small Intestinal.
Herba Portulacae, cold in nature, sweet in the mouth acid, enters the heart, liver, spleen, large intestine channel.There is the effects such as heat-clearing and toxic substances removing, diuretic dry, loose blood detumescence, cooling blood for hemostasis, dysentery relieving, can be used for treating hematodiarrhoea pus and blood, pyretic stranguria, leukorrhagia, have blood in stool, the disease such as sore swollen toxin.Compendium of Material Medica is recorded: " loose blood detumescence, sharp intestinal habitual abortion, detoxify treating stranguria, manage property after sweating due to debility "." the southern regions of the Yunnan Province book on Chinese herbal medicine ": " QI invigorating, clearing away summer-heat heat, the alleviating distention in middle-JIAO therapeutic method to keep the adverse QI flowing downwards.Laxation, removing food stagnancy band, parasite killing, treats skin ulcer congestion and swelling pain." " Treatise on Dietetic Therapy " record Herba Portulacae " cook congee dysentery relieving and dysentery ".Modern study proves that Herba Portulacae has the antibacterial action of wide spectrum, the antibacterials such as Si Shi and expense Shigella flexneri, escherichia coli, Bacillus proteus, staphylococcus aureus and virus are all had to stronger inhibitory action, there is the laudatory title of " natural antibiotics ", evident in efficacy in the digestive system disease clinical treatment such as such as bacillary dysentery, diarrhoea, ulcerative colitis.In addition, all right excited intestinal smooth muscle of Herba Portulacae, promotes intestinal peristalsis promoting, and strengthens the effects such as immunity, antitumor, blood lipid-reducing blood sugar-decreasing.
Radix Notoginseng, its sweet in the mouth, micro-hardship, warm in nature.Return liver, stomach, the heart, lung meridian, large intestine channel.The title that always has " refreshing medicine stops blooding ", Li Shizhen (1518-1593 A.D.) is called " Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) ".In supplementary Amplifications of the Compendium of Materia Medica, record: " ginseng qi-tonifying the first, Radix Notoginseng enriches blood the first, taste with and merit also etc., therefore claim Panax pseudoginseng, be the most precious person in medicine." Compendium of Material Medica cloud: " Radix Notoginseng hemostasis, loose blood, analgesic therapy." " beautiful Chinese catalpa medicine solution " cloud: " Radix Notoginseng and battalion hemostasis, the clots absorbing of promoting blood circulation, clots absorbing blood and hold back fresh blood." cure mainly various hemorrhage, breast ventral spine pain, tumbling down swells and ache, to have the stagnant person of the stasis of blood concurrently particularly suitable to hemorrhage.Radix Notoginseng clinical practice is extensive, is also one of conventional combination drug of the digestive system disease such as treatment by Chinese herbs digestive tract ulcer and ulcerative colitis.The modern pharmacological research of Radix Notoginseng is mainly concentrated on to arasaponin and aminoacid, studies have shown that Radix Notoginseng has hemostasis, antithrombotic forms and promotes hemoposieis.Radix Notoginseng has stronger antiinflammatory action simultaneously, not only there is direct anti-inflammatory effect, to histamine, acetic acid, dimethylbenzene, 5-hydroxy tryptamine, the capillary permeability that Kallidin I etc. cause raises and has obvious inhibitory action, again can be by excited Pituitary Adrenal cortex system, reduce inflammatory exudation and swollen tissue, bring into play indirect antiinflammatory action.In addition the composition such as Radix Notoginseng polysaccharide, arasaponin also has immunological enhancement.Other researchs also confirm that Radix Notoginseng also has certain effect at aspects such as analgesia, antitumor.
In view of causing, ulcerative colitis sends out the ever-increasing clinical demand of colorectal cancer incidence rate, can effectively prevent and treat ulcerative colitis according to Fructus Hordei Germinatus fiber, be based on Fructus Hordei Germinatus and prevent and treat the useful function of the component regulating gastointestinals such as gastrointestinal disease experience and Poria, Rhizoma Dioscoreae, early stage, we researched and developed with Fructus Hordei Germinatus fiber, Poria, the fine powder preparation of the common YUANMAI forming of three components of Rhizoma Dioscoreae, on this basis, also carried out corresponding Clinical and experimental study, the data and information of feedback has verified that fine the faling apart of wheat has definite curative effect for treatment irritable bowel syndrome and ulcerative colitis.We are based on gastrointestinal system disorder diagnosis and treatment experience for many years and in conjunction with some up-to-date domestic and international results of study, make every effort to improve this formula and best its treatment effect of performance, intend by improved formulations composition, proportion compatibility and processing technology, researched and developed and had more curative effect and the cheap natural Chinese medicines preparation-new fine powder preparation of wheat (component comprises Fructus Hordei Germinatus fiber, Poria, Rhizoma Dioscoreae, Herba Portulacae and Radix Notoginseng powder) of safety.The fine powder preparation of new wheat is that we are in conjunction with early-stage Study achievement, still with Fructus Hordei Germinatus fiber as key component, in formula by certain technique compatibility the components such as the Poria of medicine-food two-purpose, Rhizoma Dioscoreae, Herba Portulacae and Radix Notoginseng powder, to the ratio of each component, we have also carried out groping many times, have made corresponding adjustment according to experiment and clinical therapeutic efficacy.Accordingly, our the follow-up relevant clinical of carrying out is observed and is found, compares with the fine powder preparation of YUANMAI, and the fine powder preparation of new wheat is applied to prevention and auxiliary treatment ulcerative colitis and irritable bowel syndrome and has more clinical efficacy and do not find irritated and other side effect.Experimentation has confirmed that the fine powder preparation of new wheat has more effect at aspects such as regulating intestinal canal probiotics, treatment irritable bowel syndrome and ulcerative colitiss.
The present invention's medical material used is the medical material of medicine-food two-purpose that on ordinary days can be edible for a long time, low price and without any toxic and side effects, and have fairly obvious effect for improving intestinal microbial population.
Accompanying drawing explanation
Fig. 1 is the assay method flow chart of Fructus Hordei Germinatus fiber fineness.
The specific embodiment
Further set forth the present invention below in conjunction with specific embodiment, should be understood that following examples are only not used in and limit the scope of the invention for the present invention is described.
In the following example, method therefor if no special instructions, is conventional method.Needed material or reagent in following examples, be if no special instructions market and buy.Wherein, Radix Notoginseng is the place of production, Yunnan, or is called Yunnan Radix Notoginseng, and all the other component places of production are without specific (special) requirements.
The requirement of high-quality germinating barley: 1) sense organ: have fresh Fructus Cucumidis sativi fragrance, without musty and abnormal flavour, be held in the hand flexible, soft sense; 2) germination percentage: should be more than 95%; 3) radical bud: healthy and strong, fresh, even, radical bud number is about 5 times; Pale malt radical bud length is 1～1.5 times of wheat grain length, and the radical bud length of heavy colour Fructus Hordei Germinatus is 2～2.5 times of wheat grain length; 4) leaf bud length: germination 5d, requiring pale malt leaf bud length is accounting for more than 80% of wheat grain length 2/3～3/4, heavy colour Fructus Hordei Germinatus leaf bud leading Catholic clergymen degree is accounting for more than 80% of wheat grain length 3/4～4/5; 5) endosperm shape: Testa Tritici strip off, with thumb and forefinger afterturn endosperm, scatter if be powdery, frangible and lubricated.
For convenience, the fine powder preparation of wheat that below, component is comprised to Fructus Hordei Germinatus fiber, Poria, Rhizoma Dioscoreae is called the fine powder preparation of YUANMAI; The fine powder preparation of wheat that component is comprised to Fructus Hordei Germinatus fiber, Poria, Rhizoma Dioscoreae, Herba Portulacae and Radix Notoginseng powder is called the fine powder preparation of new wheat.
The preparation of the fine powder preparation of embodiment 1 YUANMAI:
(1) by germinating barley saccharifying processing
Choose high-quality germinating barley, go Mai Gen and selected after, at 47 ℃, adopt dry pulverization process 30min, 90 ℃ are dried to constant weight, the Fructus Hordei Germinatus after being pulverized.In brew kettle, first add suitable quantity of water (Fructus Hordei Germinatus and water quality ratio=1:4), be heated to 37 ℃, then add the Fructus Hordei Germinatus crushing, maintain (65-72 ℃) and manufacture wheat wine with dregs.The climb procedure of wheat wine with dregs temperature adopts secondary to cook method.When saccharifying finishes within the shortest time by wheat juice and wheat groove isolated by filtration.
(2), the extraction of Fructus Hordei Germinatus fiber
After wheat grain adopts indirect type heat drying method to dry, flattening, fragmentation, sieve through 40 mesh standard sieves, adopt alkali-enzyme combined techniques to extract Fructus Hordei Germinatus fiber in wheat groove, and adopt enzyme-gravimetric method to analyze its purity.
The concrete preparation method of Fructus Hordei Germinatus fiber is as follows:
1. wheat grain is dried after pulverizing, cross 40 mesh standard sieves;
2. washing is boiled: add water (solid-to-liquid ratio 1:20) boils 15min;
3.0.4% enzymolyzing alpha-amylase: 65 ℃ of enzymolysis 45min, 2 enzyme denaturing activity of 100 ℃ of boiling water washings, the centrifugal 10min of 4000r/min, taking precipitate;
4. alkali treatment: precipitate adds 3%NaOH solution by solid-to-liquid ratio 1:10 is hydrolyzed 5h at 90 ℃, to remove protein and fat, is washed to neutrality, dries to constant weight for 90 ℃;
5.H
2o
2decolouring: take a certain amount of Fructus Hordei Germinatus fiber, add 5%H by solid-to-liquid ratio 1:20
2o
2, adjust pH to 10,55 ℃ of decolouring 2h, take out rapidly, the supernatant that inclines, then add water washing, with the HCl neutralization of 1mol/L, with water rinse, sucking filtration 3 times, 65 ℃ are dried to constant weight.In whole oxidation bleaching process, add 0.75% sodium tripolyphosphate and 0.75% sodium hydrogen phosphate is got rid of metal ion disturbance.Bleaching process repeats 2～3 times.
6. cross 120 mesh standard sieves, obtain Fructus Hordei Germinatus fibrous finished product and carry out purity testing.
The mensuration of Fructus Hordei Germinatus fiber fineness
Adopt soluble fiber, insoluble fibers and total fiber amount in enzyme-gravimetric detemination Fructus Hordei Germinatus fiber.
Determination step is as shown in Figure 1:
Accurately take double sample, be stirred to completely and disperse with buffer respectively, then use successively amylase and white enzyme enzymolysis, centrifugal after washing 2 times, the filtering residue obtaining obtains insoluble fibers with 78%, 95% ethanol, washing with acetone 2 times oven dry respectively.Filtrate is precipitated with 4 times of volumes, 95% ethanol, centrifugal rear 78%, 95% ethanol, the twice → oven dry → soluble fiber of washing with acetone used respectively.
Finally we record in Fructus Hordei Germinatus fiber
Soluble fiber: 14.30%; Insoluble fibers: 60.08%; Total fiber: 74.38%.
(3), the preparation of the fine powder preparation of wheat
Selected Poria, the Rhizoma Dioscoreae prepared slices of Chinese crude drugs are removed impurity, dry, and after pulverizing, then sieve for depositing through 120 mesh standard sieves with Chinese medicine high speed disintegrator.By Fructus Hordei Germinatus fiber 18g, the ratio of Poria 12g, Rhizoma Dioscoreae 12g is added after each component, has fully mixed the preparation of the fine powder preparation of YUANMAI.
The preparation of the fine powder preparation of the new wheat of embodiment 2:
(1), by germinating barley saccharifying processing
Choose high-quality germinating barley, go Mai Gen and selected after, at 47 ℃, adopt dry pulverization process 30min, 90 ℃ are dried to constant weight, the Fructus Hordei Germinatus after being pulverized.In brew kettle, first add suitable quantity of water (Fructus Hordei Germinatus and water quality ratio=1:4), be heated to 37 ℃, then add the Fructus Hordei Germinatus crushing, maintain (65-72 ℃) and manufacture wheat wine with dregs.The climb procedure of wheat wine with dregs temperature adopts secondary to cook method.When saccharifying finishes within the shortest time by wheat juice and wheat groove isolated by filtration.
(2), the extraction of Fructus Hordei Germinatus fiber
After wheat grain adopts indirect type heat drying method to dry, flattening, fragmentation, sieve through 40 mesh standard sieves, adopt alkali-enzyme combined techniques to extract Fructus Hordei Germinatus fiber in wheat groove.(preparation and determination methods method is with embodiment 1)
(3), the preparation of the fine powder preparation of new wheat
Selected Poria, Rhizoma Dioscoreae, Herba Portulacae, the Radix Notoginseng prepared slices of Chinese crude drugs are removed impurity, dry, and after pulverizing, then sieve for depositing through 120 mesh standard sieves with Chinese medicine high speed disintegrator.According to Fructus Hordei Germinatus fiber 18g, the ratio of Poria 12g, Rhizoma Dioscoreae 12g, Herba Portulacae 9g, Radix Notoginseng powder 9g is added after each component, has fully mixed the fine loose preparation of new wheat.
(4), loosely directly make and become granule, capsule or tablet fine new wheat, or add pharmaceutic adjuvant and become Chinese patent medicine.
Embodiment 3 clinical efficacies
1.1 clinical efficacy index analysis
After the treatment of surrounding, the overall symptom integral of IBS-D patient before has obvious decline, and cure rate is 2.5%, and obvious effective rate is up to 65%, and effective percentage is 30%.An invalid example, inefficiency is 2.5%.
Integration evaluation in 1.2 intestinals
According to collected clinical efficacy result, in intestinal, symptom improvement degree can obtain following result from the change of stool character:
The fine loose group of YUANMAI:
Treat front 40 routine IBS-D patients severe (watery stool) person 18 examples of defecating, account for 45.0%; Moderate patient's (sticking with paste sample just) 22 examples, account for 55.0%; After treatment, watery stool symptom all disappears, and stool character recovers to reach normally 25 examples (62.5%), sticks with paste sample and just drops to 4 examples, and proportion drops to 10%.
The fine loose group of new wheat:
Treat front 40 routine IBS-D patients severe (watery stool) person 21 examples of defecating, account for 52.5%; Moderate patient's (sticking with paste sample just) 19 examples, account for 47.5%; After treatment, watery stool symptom all disappears, and stool character is recovered normally up to 29 examples (72.5%), sticks with paste sample and just drops to 2 examples, and proportion drops to 5%.
Symptom total mark comparison before and after table 1IBS-D patient treatment
| Group | Number of cases | Before treatment | After treatment | Difference |
| YUANMAI is fine loose | 40 | 26.00±6.61 | 10.90±3.07 | 15.10±6.17 |
| New wheat is fine loose | 40 | 25.70±7.14 | 7.93±2.97 | 17.78±4.28 |
Note: before and after IBS-D patient treatment, symptom total mark difference is carried out paired t-test, p<0.01, illustrates before and after treatment and has significant difference.Total mark after treatment lower than treatment before, proves that fine loose and fine the faling apart of new wheat of YUANMAI can significantly improve bowel symptoms, there is treatment meaning.Between group, relatively adopt two independent sample t checks, p<0.05, new wheat is fine, and loose to the effect of improving of symptom total mark, to be better than YUANMAI fine loose.
Intestinal integral contrast before and after 1.3 treatments
Intestinal integral contrast before and after table 2IBS-D patient treatment
| Group | Number of cases | Before treatment | After treatment | Difference |
| YUANMAI is fine loose | 40 | 19.65±4.90 | 10.90±3.07 | 12.90±5.24 |
| New wheat is fine loose | 40 | 19.48±5.35 | 7.93±2.97 | 13.80±3.21 |
Note: before and after IBS-D patient treatment, intestinal integration differential carries out paired t-test, p<0.01, illustrates before and after treatment and has significant difference.Total mark after treatment lower than treatment before, proves that fine the faling apart of the fine loose and new wheat of YUANMAI improve bowel symptoms and have treatment meaning.Between group, relatively adopt two independent sample t checks, p<0.05, new wheat is fine, and loose to the effect of improving of bowel symptoms, to be better than YUANMAI fine loose.
2 quality of life efficacy evaluation
Relative analysis before and after the treatment of 2.1 quality of lifes
The analysis of the fine loose treatment Life For The Residents of Three Gorges Reservoir Before of the routine IBS-D patient's YUANMAI of table 340
| SF-36 dimension | Before treatment (n=40) | After treatment (n=40) | Difference |
| PF | 86.1±10.8 | 87.8±10.3 | 1.1±2.4 |
| RP | 57.5±24.8 | 86.9±13.8 | 29.4±21.1 |
| BP | 56.8±12.6 | 76.5±10.3 | 19.7±11.4 |
| GH | 38.7±11.5 | 45.5±14.1 | 6.8±7.1 |
| VT | 42.3±10.4 | 51.3±12.3 | 9.0±9.0 |
| SF | 66.6±10.7 | 89.1±12.7 | 22.5±12.7 |
| RE | 58.3±33.5 | 77.5±21.9 | 19.2±19.8 |
| MH | 56.8±12.6 | 62.0±11.9 | 9.7±7.1 |
The analysis of the fine loose treatment Life For The Residents of Three Gorges Reservoir Before of the routine IBS-D patient Xin Mai of table 440
| SF-36 dimension | Before treatment (n=40) | After treatment (n=40) | Difference |
| PF | 88.8±6.9 | 90.8±5.4 | 2.0±2.5 |
| RP | 55.0±36.3 | 85.6±20.3 | 30.6±20.3 |
| BP | 54.2±8.4 | 76.3±9.3 | 22.1±7.2 |
| GH | 31.8±10.4 | 38.6±7.5 | 8.4±5.6 |
| VT | 39.3±8.8 | 49.3±10.9 | 10.3±4.9 |
| SF | 52.5±15.2 | 75.0±15.0 | 22.8±6.9 |
| RE | 40.8±32.5 | 61.7±26.8 | 20.8±18.0 |
| MH | 55.6±11.2 | 65.6±9.1 | 10.0±5.7 |
Note: difference is carried out to Wilcoxon rank test analysis and draw, the equal p<0.05 of all dimensions, has statistical significance.
2.2 quality of lifes improved and clinical symptoms is improved correlation analysis
The fine loose treatment quality of life-symptom of the routine IBS-D patient's YUANMAI of table 540 is improved correlation analysis
Note: the improvement of symptom curative effect and quality of life is certain being correlated with, the wherein relevant not statistically significant (P>0.05) of part
The fine loose treatment quality of life-symptom of the routine IBS-D patient Xin Mai of table 640 is improved correlation analysis
Note: the improvement of symptom curative effect and quality of life is certain being correlated with, the wherein relevant not statistically significant (P>0.05) of part
Newer wheat fine loose and the fine loose concrete outcome that IBS-D quality of life and symptom are improved of YUANMAI, show that new wheat is fine to be loosely dispersed in and to improve patient's symptom and there is further raising raising life aspect compared with wheat fibre.In addition,, in clinical trial process, all do not find that IBS-D patient has anaphylaxis and do not tolerate phenomenon with the fine loose powder of new wheat fine the faling apart of YUANMAI, does not find obvious toxic-side effects.
By Fructus Hordei Germinatus fiber being regulated to the research of UC mouse intestinal flora impact, find to treat after 2 weeks through Fructus Hordei Germinatus fiber in addition, enterobacteria and enterococcus quantity reduce, and bacillus bifidus and lactobacillus quantity obviously increase.Research shows that Fructus Hordei Germinatus fiber has good regulating and controlling effect to UC mouse intestinal flora, can better regulate ulcerative colitis cytokine levels, and the signal conduction of nuclear factor KB is had to good regulating and controlling effect; Regulate, improve the effects such as immunity by its flora, can auxiliary treatment ulcerative colitis and dependency colorectal cancer, the side effect that alleviates hormone and immunosuppressant.Fructus Hordei Germinatus fiber affects concrete outcome in table 7 to UC rat cecal content butyric acid bacteria content.
The comparison of table 7 each group intestine in rats content butyric acid bacteria content (lgCFU/g)
Note: * represents and normal group comparison, P<0.05; ﹟ represents and model group comparison, P<0.05; ☆ represents and matched group comparison, P<0.05
According to NI of China relevant regulations, the fine powder preparation adult of YUANMAI (60kg) suitable intake is 30g/d, and the dose,equivalent that we calculate 200g left and right experimental rat according to this absorption ratio of 0.5g/kg is about 1g/d.For determining Fructus Hordei Germinatus fiber best compatibility dosage in the fine loose prescription of wheat, we have observed Fructus Hordei Germinatus fiber: Poria: Rhizoma Dioscoreae=1:1:1; 2:1:1; The concrete outcome that tri-different proportions of 3:1:1 form the impact on UC intestine in rats content butyric acid bacteria content is in table 8.
The comparison of table 8 each group intestine in rats content butyric acid bacteria content (lgCFU/g)
According to experimental result, finally we have determined that the fine loose best proportion compatibility of wheat is 3:2:2 and has carried out accordingly corresponding clinical trial and zoopery.
For determining the fine loose optimum proportion compatibility of new wheat, according to the combination principle of Chinese medicine monarch, new wheat fine loose in we still take Fructus Hordei Germinatus fiber as monarch drug (A), Poria, Rhizoma Dioscoreae are ministerial drug (B1, B2), Herba Portulacae, Radix Notoginseng powder are adjuvant (C1, C2).According to NI of China relevant regulations, the fine powder preparation adult of new wheat (60kg) suitable intake is 30g/d, the dose,equivalent that we calculate 200g left and right experimental rat according to this absorption ratio of 0.5g/kg is about 1g/d, in experimentation, calculates dosage according to rat ABW after modeling.We have designed 1,2,3 three level and have been respectively 0.3g, 0.2g, 0.15g, and according to the following experimental program of orthogonal, the concrete outcome of the impact of the fine loose different compatibilities of new wheat on UC intestine in rats content butyric acid bacteria content is in table 9.
The comparison of the different proportion compatibilities of table 9 to UC intestine in rats content butyric acid bacteria content (lgCFU/g)
Note: due to the dose,equivalent that the each medicine weight of experimental program 1 summation 1.5g obviously exceedes 1g, rejected, with 1 ˊ scheme implementation.Experimental result prompting is in the time that the fine loose middle Fructus Hordei Germinatus fiber of new wheat, Poria, Rhizoma Dioscoreae, Herba Portulacae, Radix Notoginseng powder content are respectively 0.3g, 0.2g, 0.2g, 0.15g, 0.15g, to adjustment the best of UC intestine in rats content butyric acid bacteria content, according to experimental result, finally we have determined that the fine loose best proportion compatibility of new wheat is Fructus Hordei Germinatus fiber (30%), Poria (20%), Rhizoma Dioscoreae (20%), Herba Portulacae (15%), Radix Notoginseng powder (15%).New wheat is fine loosely affects concrete outcome in table 10 to UC intestine in rats butyric acid bacteria content.
The comparison of the each group of table 10 intestine in rats butyric acid bacteria content
Note: * represents and normal group comparison, P<0.05; ﹟ represents and model group comparison, P<0.05; ☆ represents and matched group comparison, P<0.05; △ represents to compare with the fine loose group of YUANMAI, P<0.05.
Claims (10)
1. the fine powder preparation of wheat, the crude drug that comprises following portions by weight: Fructus Hordei Germinatus fiber 4-7 part, Poria 4-5 part, Rhizoma Dioscoreae 4-5 part.
2. the fine powder preparation of wheat according to claim 1, is characterized in that: the crude drug that comprises following portions by weight: 6 parts of Fructus Hordei Germinatus fibers, 4 parts, Poria, 4 parts of Rhizoma Dioscoreaes.
3. the fine powder preparation of wheat according to claim 1, is characterized in that: the crude drug by following portions by weight forms: Fructus Hordei Germinatus fiber 4-7 part, Poria 4-5 part, Rhizoma Dioscoreae 4-5 part, Herba Portulacae 2-3 part, Radix Notoginseng powder 2-3 part.
4. the fine powder preparation of wheat according to claim 3, is characterized in that: the crude drug by following portions by weight forms: 6 parts of Fructus Hordei Germinatus fibers, 4 parts, Poria, 4 parts of Rhizoma Dioscoreaes, 3 parts of Herba Portulacaes, 3 parts of Radix Notoginseng powder.
5. the preparation method of the fine powder preparation of the arbitrary described wheat of claim 1-4, comprises the steps:
(1), by germinating barley saccharifying processing
Choose high-quality germinating barley, go Mai Gen and selected after, at 47 ℃, adopt dry pulverization process 30min, 90 ℃ are dried to constant weight, and the Fructus Hordei Germinatus after being pulverized first adds suitable quantity of water in brew kettle, Fructus Hordei Germinatus and water quality ratio=1:4, be heated to 37 ℃, then add the Fructus Hordei Germinatus crushing, maintain 65-72 ℃ and manufacture wheat wine with dregs, the climb procedure of wheat wine with dregs temperature adopts secondary to cook method, when saccharifying finishes within the shortest time by wheat juice and wheat groove isolated by filtration;
(2), the extraction of Fructus Hordei Germinatus fiber
After wheat grain adopts indirect type heat drying method to dry, flattening, fragmentation, sieve through 40 mesh standard sieves, adopt alkali-enzyme combined techniques to extract Fructus Hordei Germinatus fiber in wheat groove;
(3), the preparation of the fine powder preparation of wheat
Selected Poria, Rhizoma Dioscoreae, or the prepared slices of Chinese crude drugs such as Poria, Rhizoma Dioscoreae, Herba Portulacae, Radix Notoginseng, remove respectively impurity, dry, after pulverizing with Chinese medicine high speed disintegrator, then sieve for depositing through 120 mesh standard sieves, add in Fructus Hordei Germinatus fiber according to proportioning, fully mixed the fine loose preparation of wheat.
6. the fine powder preparation of the arbitrary described wheat of claim 1-4 is for the preparation of the purposes of improving intestinal microbial population medicine.
7. the fine powder preparation of the arbitrary described wheat of claim 1-4 is for the preparation of the purposes that prevents and/or treats ulcerative colitis medicine.
8. the fine powder preparation of the arbitrary described wheat of claim 1-4 is for the preparation of the purposes that prevents and/or treats irritable bowel syndrome medicine.
9. purposes according to claim 8, is characterized in that: described irritable bowel syndrome is diarrhea type irritable bowel syndrome.
10. the dosage form of the fine powder preparation of wheat claimed in claim 1 is granule, capsule or tablet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410138907.4A CN103877446B (en) | 2014-04-09 | 2014-04-09 | Wheat fiber powder preparation and preparation method and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410138907.4A CN103877446B (en) | 2014-04-09 | 2014-04-09 | Wheat fiber powder preparation and preparation method and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103877446A true CN103877446A (en) | 2014-06-25 |
| CN103877446B CN103877446B (en) | 2017-05-17 |
Family
ID=50946782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410138907.4A Active CN103877446B (en) | 2014-04-09 | 2014-04-09 | Wheat fiber powder preparation and preparation method and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103877446B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113499402A (en) * | 2021-08-27 | 2021-10-15 | 赵站周 | Traditional Chinese medicine composition for treating irritable bowel syndrome and preparation method thereof |
-
2014
- 2014-04-09 CN CN201410138907.4A patent/CN103877446B/en active Active
Non-Patent Citations (4)
| Title |
|---|
| 张达荣等: ""肠易激综合征患者服用酪酸菌制剂前后肠道菌群状况"", 《中国微生态学杂志》 * |
| 蔡淦等: "《肠易激综合征诊治》", 31 May 2006, 上海世纪出版股份有限公司、上海科学技术出版社 * |
| 赵斌: ""溃疡性结肠炎的中药治疗"", 《中国民间疗法》 * |
| 郑红斌等: ""麦芽纤维对溃疡性结肠炎大鼠盲肠内酪酸菌及血液中酪酸含量的影响"", 《中华中医药杂志（原中国医药学报）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113499402A (en) * | 2021-08-27 | 2021-10-15 | 赵站周 | Traditional Chinese medicine composition for treating irritable bowel syndrome and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103877446B (en) | 2017-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102198262B (en) | Chinese medicine preparation for treating infant rotavirus enteritis and preparation method thereof | |
| CN101297947A (en) | Medicine powder for treating uncomfortable intestine and stomach and preparation thereof | |
| CN103005264B (en) | Chitosan oligosaccharide-containing healthcare food and preparation method thereof | |
| CN101757083B (en) | Costunolide-containing traditional Chinese medicine composition for treating gastrointestinal diseases and preparation method thereof | |
| CN108310295B (en) | Traditional Chinese medicine composition with spleen and stomach regulating function and preparation method thereof | |
| CN106490558A (en) | A kind of health-care honey and preparation method thereof | |
| CN102091279B (en) | Traditional Chinese medicine composition for treating duodenal ulcer | |
| CN105435084B (en) | Traditional Chinese medicine for tonifying spleen and removing dampness and preparation method thereof | |
| CN101468173B (en) | Chinese medicinal composition for treating children's indigestion and preparation method thereof | |
| CN103877446B (en) | Wheat fiber powder preparation and preparation method and use thereof | |
| CN102988747B (en) | Aristida triseta electuary for reducing hypertension, hyperglycemia and hyperlipidemia and preparation method thereof | |
| CN106334065A (en) | Edible and medicinal traditional Chinese medicine formula used for treating gynecological diseases and having beauty effect and production process | |
| CN112022984A (en) | Preparation for removing food retention, preparation method and application thereof | |
| CN105362914A (en) | Traditional Chinese medicinal composition for treating chronic gastritis | |
| CN103142918A (en) | Chinese patent drug for treating infantile diarrhea | |
| CN101461854B (en) | Colitis treating Chinese medicine preparation | |
| CN107281414A (en) | A kind of dietic medicinal tea and preparation method | |
| CN100998785B (en) | Traditional Chinese medicine composition for treating gastrointestinal disease and its preparing method | |
| CN106727681A (en) | A kind of pharmaceutical composition for treating constipation in children | |
| CN105079196A (en) | Health soup capable of promoting milk secretion and preparation method of health soup | |
| CN105962352A (en) | Food medicine for protecting male prostate gland and preparation method thereof | |
| CN102885988A (en) | Traditional Chinese medicine (TCM) for treating pancreatic cancers | |
| CN103861034B (en) | A kind of Chinese medicine helped digestion and preparation method thereof | |
| CN104740013A (en) | Traditional Chinese medicine composition for treating malignant tumors | |
| CN104474381B (en) | A kind of Chinese medicine composition of delaactation and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |