CN103875846A - Bagged antrodia camphorata strain and pseudostellaria heterophylla tea and preparation method thereof - Google Patents
Bagged antrodia camphorata strain and pseudostellaria heterophylla tea and preparation method thereof Download PDFInfo
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Abstract
The invention relates to bagged antrodia camphorata strain and pseudostellaria heterophylla tea and a preparation method of the bagged antrodia camphorata strain and pseudostellaria heterophylla tea, and in particular discloses application of antrodia camphorata to preparation of the bagged antrodia camphorata strain and pseudostellaria heterophylla tea by fermentation and a method for preparing the bagged antrodia camphorata strain and pseudostellaria heterophylla tea by fermenting pseudostellaria heterophylla by antrodia camphorata. An antrodia camphorata strain is bought from ATCC (American Type Culture Collection) and has the number of 200183. The preparation method comprises the following steps: performing culture fermentation in the pseudostellaria heterophylla as a main matrix raw material by taking the antrodia camphorata as the fermenting strain, post-processing and bagging to obtain the bagged antrodia camphorata strain and pseudostellaria heterophylla tea. The contents of crude polysaccharides, total triterpenes and total amino acids in tea soup are all greatly increased in comparison with references; the tea is clear and transparent in soup color and is mellow and full in mouth feel, has the specific fruit aroma of the antrodia camphorata, and is light and pleasant; the original special smell of the pseudostellaria heterophylla is greatly improved, so that the tea is relatively easy to accept.
Description
Technical field
The present invention relates to a kind of bacterium ginseng fermentation bag and make tea and preparation method thereof, relate in particular to a kind of fermented tea and processing technology thereof containing camphor tree sesame and radix pseudostellariae, belong to tea substitute field.
Background technology
Radix pseudostellariae is the dried root of pinkwort caryophyllaceous ginseng [Pseud ostellaria heterophylla (Miq.) Pax ex Pax et Hoffm.], sweet, the micro-hardship of its taste, property flat, return spleen lung channel, can help gas the moon, promote the production of body fluid to quench thirst, effect and ginseng are close, but strength a little less than, the efficacy of a drug is gentle, to mend and to be good at clearly, all-ages.Research shows, radix pseudostellariae is rich in nutrition and the functional component (Qin Minjian such as carbohydrate, cyclic peptide, amino acids, saponins, trace element, Yu Yongbang, Huang Wenzhe, Deng. the attributional analysis of different places of production radix pseudostellariae. R&D of modern TCM and practice, 2005,19 (5): 29-32; Lin Mao, Zheng Jiong, Yang Lin, Deng. chemical composition analysis in the radix pseudostellariae of the different places of production. Food Science, 2012,33 (2): 204-207), have antifatigue, anti-oxidant, anti-stress, hypoglycemic, strengthen remarkable efficacy (the Wong C K such as immunity, Leung K N, Fung K P. The immunostimulating activities of anti-tumor polysaccharides from Pseudostellaria heterophylla. Immunopharmacology, 1994,28 (1): 47-54; NG T B, LIU F, WANG H X. The antioxldant efects of aqueous and organic extracts of Panax quinquefolium, Panax notoginseng, Codonopsis pilosula, Pseudostella riaheterophylla and Glehnia littoralis. Ethnopharmacol, 2004,93 (23): 285-288; Wu Chaofeng, Lin Yanquan. the progress of medicinal plant radix pseudostellariae. University Of Agriculture and Forestry In Fujian's journal (natural science edition), 2004,33 (4): 426-430), having high edible and medical value, is to have simply to replenish qi to invigorate the spleen and the effect such as promoting production of body fluid and nourishing the lung and nutritious preciousness are mended clearly good merchantable brand.
Before 20 century 70s, radix pseudostellariae, take wild as main, has distribution on China East China, Central China, North China and northwest and other places; Rear expansion cultivation, now in Fujian, all there are scale plantation and cultivation in the ground such as Shandong, Jiangsu and Anhui, resource is very abundant, carry out the edible developmental research of radix pseudostellariae, the demand that can boost consumption, improves making full use of of its resource, for Development area economy, the transformation of the mode of promoting economic development, has meaning of crucial importance.Be used for Baoshang or effective component extracting as meals raw material and make traditional edible way that tablet, oral liquid etc. are radix pseudostellariaes, along with the continually developing of the radix pseudostellariae utilization of resources, the raising of people's quality of the life and the expansion of the market demand, take radix pseudostellariae as primary raw material and the help health care tea of formula etc. constantly develop, there is great application prospect because it is easy to carry and eats.
Chinese patent CN103404676A discloses a kind of preparation method of radix pseudostellariae tea, and this radix pseudostellariae tea only has radix pseudostellariae to form, and method comprises raw material selection, grade screening, cleaning, oven dry, frying processing, pulverizing, preservation and packaging.
The white tea of radix pseudostellariae is made up of according to certain proportioning Zherong radix pseudostellariae and Fuding white tea, not only keep the radioresistance of white tea but also increased the health care (Cai Binxin of radix pseudostellariae tonifying Qi benefit spleen and nourishing Yin and promoting production of body fluid, Chen Aiping, Lin Jinke, Deng. the protective action of the white tea of radix pseudostellariae to radiation. Ningde college of education journal (natural science edition), 2013,25 (1): 21-27.).
The prescription of ginseng wheat tea is radix pseudostellariae 9g, light wheat 15g, 20 pieces of red dates, have qi-supplementing, blood-engendering effect (Zhang Jie, in Hua Sheng, stragglers and disbanded soldiers' base. the edible and medical value of Zherong radix pseudostellariae. Strait Pharmaceutical Journal, 2011,23 (5): 48-50).
Chinese patent CN10208492A discloses the preparation method of honeysuckle radix pseudostellariae tea, and this preparation method comprises that raw material chooses and prepare burden, extracting honeysuckle 6g and radix pseudostellariae 15g, and both pulverize rear mixing, obtain honeysuckle radix pseudostellariae tea, have the effect of tonifying Qi and heat-clearing.
The radix pseudostellariae teas goods that these develop, are the conventional pulverization process of raw material, and are the simple mixing of radix pseudostellariae and other food (or medicine).And only improving the degree of separating out of radix pseudostellariae tea functional component by pulverizing, effect is limited, and pulverizes the meticulous easy millet paste clarity that affects; In addition, radix pseudostellariae medicinal material itself contains the compounds such as methyl mercaptan, dimethyl disulfide, little molecule furans and furfural, (money is big can to give out a kind of seemingly " BHC " sample characteristic odor, Han Le, Liu Xunhong etc. the HSGC-MS analysis and research of radix pseudostellariae medicinal material characteristic odor composition. R&D of modern TCM and practice, 2010,24 (5): 25-27), affect mouthfeel, simple mixing can not improve the original special odor of radix pseudostellariae significantly.
Antrodia camphorata is the distinctive rare edible mushroom in Taiwan, have calm the nerves, gentleness disappears long-pending, removing toxicity for detumescence, anti-oxidant, immunity strengthens, protect the liver, effect (Li-Shian Shi such as antitumor, Chih-Hua Chao, De-Yang Shen, et al. Biologically active constituents from the fruiting body of Taiw anofungus camphorates. Bioorganic & Medicinal Chemistry, 2011,19:677-683), can decompose and utilize the matrix such as lignin and cellulose to grow.
Utilize radix pseudostellariae raw material to carry out fermented and cultured to Antrodia camphorata, make bacterium ginseng fermentation bag and make tea.Functional component content in fermented tea millet paste is significantly improved; Meanwhile, the quality of tea product be improved significantly, millet paste presents good color and luster, mouthfeel is mellow and full, the distinctive fruital of Antrodia camphorata has improved the original special odor of radix pseudostellariae, make us being easier to accept.
summary of the inventionthe object of the present invention is to provide a kind of Antrodia camphorata (
antrodia camphorata,purchased from ATCC, deposit number is 200183) prepare radix pseudostellariae tea in fermentation, promote radix pseudostellariae tea product functional component to separate out and improve the application aspect radix pseudostellariae tea product smell.
The present invention also aims to provide a kind of Antrodia camphorata fermentation radix pseudostellariae to prepare the method that bacterium ginseng fermentation bag is made tea.
Antrodia camphorata fermentation radix pseudostellariae is prepared the method that bacterium ginseng fermentation bag is made tea, and comprises the following steps:
(1) raw material is processed and proportioning:
Radix pseudostellariae is cleaned, and spreads out fully and dries, and pulverizes and cross 20 mesh sieves; By radix pseudostellariae powder, analysis for soybean powder, corn flour, edible plaster mixed preparing material, sterilizing in autoclave, cooling rear for subsequent use;
(2) bacterial classification preparation:
Antrodia camphorata slant strains (big or small about 5mm × 5mm) is seeded to potato dextrose agar (PDA), in constant temperature and humidity incubator, cultivates, obtain the dull and stereotyped bacterial classification of Antrodia camphorata;
(3) inoculation fermentation:
In superclean bench, just the dull and stereotyped bacterial classification of Antrodia camphorata divides the fritter into about 2.5mm × 2.5mm, is seeded in the fermentation raw material after sterilization treatment, and fully mixes with raw material; Be placed in constant temperature and humidity incubator and carry out pure culture;
(4) post processing of fermented tea and packing:
After fermentation ends, take out fermentate and dry, crush caking and fully mix, be distributed into food-grade filter paper tea bag bag (2 g/bag) sealing, make bacterium ginseng fermentation bag and make tea.
Wherein the raw material proportioning described in step (1) is that every 100g material is containing radix pseudostellariae powder 85 g-97 g, analysis for soybean powder 1 g-10 g, corn flour 1 g-10 g, edible plaster 1 g-5 g; It is 40 g-65 g that described material moisture is controlled at every 100g water content.
Wherein condition of culture described in step (2) is 27 ℃ of cultivation 12 d; Described PDA culture medium comprises following component: potato 200 g, and glucose 20 g, agar 20 g, water is settled to 1000 mL, natural pH.
Wherein pure culture condition described in step (3) is 25 ℃-32 ℃ cultivation 15 d-30 d.
Wherein described in step (4), dry extremely every 100 g moisture content of material and be less than 5 g; It is 2 g/bags that described bacterium ginseng fermentation bag is made tea.
Beneficial effect:
Existing report or disclosed radix pseudostellariae tea preparation method, is the conventional pulverization process of raw material, and the simple mixing of radix pseudostellariae and other food (or medicine), there is no radix pseudostellariae is fermented and prepares the relevant achievement report of fermented tea.Radix pseudostellariae medicinal material itself contains the compounds such as methyl mercaptan, dimethyl disulfide, little molecule furans and furfural, can give out a kind of like " BHC " sample characteristic odor, mouthfeel when impact is taken, the processing simply mixing with other compositions can not improve the original special odor of radix pseudostellariae significantly.
Thereby the present invention is fermented and is prepared fermented tea radix pseudostellariae, the quality of tea product be improved significantly, millet paste presents good color and luster, mouthfeel is mellow and full, the distinctive fruital of Antrodia camphorata has improved the original special odor of radix pseudostellariae, make us being easier to accept; And the thick polysaccharide of fermented tea, the functional components such as total triterpene and total amino acid more easily disengage due to the degradation of Antrodia camphorata to radix pseudostellariae cellulose and lignin etc., and the content in millet paste contrasts and is significantly improved.
The specific embodiment
The present invention is further illustrated in conjunction with the embodiments now.
embodiment 1: the preparation of raw material:
(1) PDA culture medium preparation
Potato 200 g, glucose 20 g, agar 20 g, water is settled to 1000 mL, natural pH.115 ℃, 0.1 MPa autoclaving 20 min.
(2) Antrodia camphorata actication of culture
By the Antrodia camphorata of Refrigerator store (
antrodia camphorate,200183) bacterial classification is seeded on PDA slant medium in superclean bench, in 27 ℃ of constant temperature and humidity incubators, cultivates 10 d, obtains slant activation bacterial classification.
(3) the dull and stereotyped bacterial classification preparation of Antrodia camphorata
Antrodia camphorata slant activation bacterial classification (big or small about 5mm × 5mm) is seeded to the dull and stereotyped central authorities of PDA, in 27 ℃ of constant temperature and humidity incubators, cultivates 12 d, obtain the dull and stereotyped bacterial classification of Antrodia camphorata.
(4) preparation of radix pseudostellariae powder
Select materials: select without disease and pest, without going mouldy, radix pseudostellariae of uniform size, for subsequent use.
Cleaning is tedded: the radix pseudostellariae of select is washed with clear water, remove the floating ash of surperficial earth etc., cleaned radix pseudostellariae is evenly spread out in bamboo plaque and ted, note ventilating, it is parched naturally.
Pulverize and sieve: the radix pseudostellariae naturally parching pulverizer is pulverized, crossed 20 mesh sieves and obtain radix pseudostellariae powder, for subsequent use.
embodiment: 2:
Get radix pseudostellariae powder 85 g, analysis for soybean powder 10 g, corn flour 10 g, the proportioning of edible plaster 5 g is carried out raw material preparation, and controlling water content is 40 g/100 g materials, and sterilizing in autoclave is cooling.In superclean bench, with inoculation shovel, dull and stereotyped Antrodia camphorata in embodiment 1 bacterial classification is seeded in the fermentation raw material after sterilization treatment, and fully mixes with raw material.Postvaccinal material is placed in to 25 ℃ of constant temperature and humidity incubators and carries out pure culture, the time is 15 d.After fermentation ends, take out fermentate and dry to moisture and be less than 5 g/100 g materials, crush caking and fully mix, be distributed into food-grade filter paper tea bag bag (2 g/bag) sealing, make bacterium and join fermentation bag and make tea.
embodiment 3:
Get radix pseudostellariae powder 95 g, analysis for soybean powder 3 g, corn flour 1 g, the proportioning of edible plaster 1 g is carried out raw material preparation, and controlling water content is 55 g/100 g materials, and sterilizing in autoclave is cooling.In superclean bench, with inoculation shovel, dull and stereotyped Antrodia camphorata in embodiment 1 bacterial classification is seeded in the fermentation raw material after sterilization treatment, and fully mixes with raw material.Postvaccinal material is placed in to 25 ℃ of constant temperature and humidity incubators and carries out pure culture, the time is 20 d.After fermentation ends, take out fermentate and dry to moisture and be less than 5 g/100 g materials, crush caking and fully mix, be distributed into food-grade filter paper tea bag bag (2 g/bag) sealing, make bacterium and join fermentation bag and make tea.
embodiment 4:
Get radix pseudostellariae powder 90 g, analysis for soybean powder 5 g, corn flour 3 g, the proportioning of edible plaster 2 g is carried out raw material preparation, and controlling water content is 45 g/100 g materials, and sterilizing in autoclave is cooling.In superclean bench, with inoculation shovel, dull and stereotyped Antrodia camphorata in embodiment 1 bacterial classification is seeded in the fermentation raw material after sterilization treatment, and fully mixes with raw material.Postvaccinal material is placed in to 32 ℃ of constant temperature and humidity incubators and carries out pure culture, the time is 18 d.After fermentation ends, take out fermentate and dry to moisture and be less than 5 g/100 g materials, crush caking and fully mix, be distributed into food-grade filter paper tea bag bag (2 g/bag) sealing, make bacterium and join fermentation bag and make tea.
embodiment 5:
Get radix pseudostellariae powder 97 g, analysis for soybean powder 1 g, corn flour 1 g, the proportioning of edible plaster 1 g is carried out raw material preparation, and controlling water content is 65 g/100 g materials, and sterilizing in autoclave is cooling.In superclean bench, with inoculation shovel, dull and stereotyped Antrodia camphorata in embodiment 1 bacterial classification is seeded in the fermentation raw material after sterilization treatment, and fully mixes with raw material.Postvaccinal material is placed in to 27 ℃ of constant temperature and humidity incubators and carries out pure culture, the time is 30 d.After fermentation ends, take out fermentate and dry to moisture and be less than 5 g/100 g materials, crush caking and fully mix, be distributed into food-grade filter paper tea bag bag (2 g/bag) sealing, make bacterium and join fermentation bag and make tea.
Tea-water proportion by the tea in bag making in embodiment 2-5 by 1: 50, boiling water brews 5 min, does not connect contrasting together of bacterium with corresponding, and millet paste is evaluated, and outcome record is as follows:
Table 1 fermented tea millet paste quality and functional component content
Claims (6)
1. bacterium ginseng fermentation bag is made tea, and it is characterized in that, utilizes radix pseudostellariae raw material to the Antrodia camphorata preparation of fermenting.
2. the preparation method that bacterium ginseng fermentation bag is made tea, is characterized in that, carries out according to following steps:
(1) raw material is processed and proportioning:
Radix pseudostellariae is cleaned, and spreads out fully and dries, and pulverizes and cross 20 mesh sieves; By radix pseudostellariae powder, analysis for soybean powder, corn flour, edible plaster mixed preparing material, sterilizing in autoclave, cooling rear for subsequent use;
(2) bacterial classification preparation:
Antrodia camphorata slant strains is seeded to PDA culture medium, in constant temperature and humidity incubator, cultivates, obtain the dull and stereotyped bacterial classification of Antrodia camphorata;
(3) inoculation fermentation:
In superclean bench, dull and stereotyped Antrodia camphorata bacterial classification is divided into fritter, be seeded in the fermentation raw material after sterilization treatment, and fully mix with raw material; Be placed in constant temperature and humidity incubator and carry out pure culture;
(4) post processing of fermented tea and packing:
After fermentation ends, take out fermentate and dry, crush caking and fully mix, be distributed into food-grade filter paper tea bag bag sealing, make bacterium ginseng fermentation bag and make tea.
3. the preparation method that a kind of bacterium ginseng fermentation bag according to claim 2 is made tea, it is characterized in that, the raw material proportioning described in step (1) is that every 100g material is containing radix pseudostellariae powder 85 g-97 g, analysis for soybean powder 1 g-10 g, corn flour 1 g-10 g, edible plaster 1 g-5 g; It is 40 g-65 g that described material moisture is controlled at every 100g water content.
4. the preparation method that a kind of bacterium ginseng fermentation bag according to claim 2 is made tea, is characterized in that, condition of culture described in step (2) is 27 ℃ and cultivates 12 d; Described PDA culture medium comprises following component: potato 200 g, and glucose 20 g, agar 20 g, water is settled to 1000 mL, natural pH.
5. the preparation method that a kind of bacterium ginseng fermentation bag according to claim 2 is made tea, is characterized in that, pure culture condition described in step (3) is 25 ℃-32 ℃ and cultivates 15 d-30 d.
6. the preparation method that a kind of bacterium ginseng fermentation bag according to claim 2 is made tea, is characterized in that, dries extremely every 100 g moisture content of material and be less than 5 g described in step (4); It is 2 g/bags that described bacterium ginseng fermentation bag is made tea.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104472786A (en) * | 2014-11-21 | 2015-04-01 | 柳州市天姿园艺有限公司 | Preparation method of antrodia tea composition |
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| TW200942180A (en) * | 2008-04-03 | 2009-10-16 | Wen-Hu Liao | Manufacturing method of Antrodia Cinnamomea tea bag |
| TW201218945A (en) * | 2010-11-11 | 2012-05-16 | Univ Nat Pingtung Sci & Tech | A culture method of Djulis (Chenopodium formosanum) for increasing the GABA content |
| TW201221065A (en) * | 2011-12-22 | 2012-06-01 | Ting-Chien Liu | Method for producing Antrodia cinnamomea tea leaves |
| TW201402018A (en) * | 2012-07-05 | 2014-01-16 | Ting-Chien Liu | Taiwanofungus Camphorates-containing cereal product and manufacturing method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200942180A (en) * | 2008-04-03 | 2009-10-16 | Wen-Hu Liao | Manufacturing method of Antrodia Cinnamomea tea bag |
| TW201218945A (en) * | 2010-11-11 | 2012-05-16 | Univ Nat Pingtung Sci & Tech | A culture method of Djulis (Chenopodium formosanum) for increasing the GABA content |
| TW201221065A (en) * | 2011-12-22 | 2012-06-01 | Ting-Chien Liu | Method for producing Antrodia cinnamomea tea leaves |
| TW201402018A (en) * | 2012-07-05 | 2014-01-16 | Ting-Chien Liu | Taiwanofungus Camphorates-containing cereal product and manufacturing method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472786A (en) * | 2014-11-21 | 2015-04-01 | 柳州市天姿园艺有限公司 | Preparation method of antrodia tea composition |
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