CN103865858A - Simple spread cultivation method for photosynthetic bacteria for treating landscape water - Google Patents
Simple spread cultivation method for photosynthetic bacteria for treating landscape water Download PDFInfo
- Publication number
- CN103865858A CN103865858A CN201410122552.XA CN201410122552A CN103865858A CN 103865858 A CN103865858 A CN 103865858A CN 201410122552 A CN201410122552 A CN 201410122552A CN 103865858 A CN103865858 A CN 103865858A
- Authority
- CN
- China
- Prior art keywords
- photosynthetic bacterium
- photosynthetic
- landscape water
- bacterium
- water processing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a simple spread cultivation method for photosynthetic bacteria for treating landscape water, belonging to the technical field of biology and being used for solving the problem of overhigh use cost of the traditional photosynthetic bacteria for treating landscape water. The method comprises the following steps of firstly, providing a photosynthetic bacterium strain; secondly, preparing a bacterium culture medium; next, inoculating photosynthetic bacterium strains to the bacterium culture medium according to a reserved number to obtain a photosynthetic bacterium inoculation solution; finally, placing the photosynthetic bacterium inoculation solution under the sun to culture for reserved days to obtain a photosynthetic bacterium fermentation solution, wherein 20-25% of photosynthetic bacterium fermentation solution can be retained for the photosynthetic bacterium strain, the rest of the photosynthetic bacterium fermentation solution can be directly used for treating landscape water on site, and the original photosynthetic bacterium strain can be repeatedly cultured 2-3 times. By using the method, not only is the transportation cost of a photosynthetic bacterium solution reduced, but also the quality stability of the photosynthetic bacterium solution for treating the landscape water is ensured.
Description
Technical field
The invention belongs to biological technical field, relate in particular to a kind of simple and easy propagation method of photosynthetic bacterium of landscape water processing.
Background technology
Photosynthetic bacterium be a class using light as the energy, can under anaerobism illumination or aerobic dark condition, utilize organism, sulfide, ammonia of occurring in nature etc. to carry out photosynthetic microorganism as the hydrogen donor carbon source of holding concurrently.The effect of photosynthetic bacterium in sewage fermentative processing becomes increasingly conspicuous.For example photosynthetic bacterium (capsula Rhodopseudomonas) can be converted into Carcinogenic Nitrosamines nontoxic compound, organic waste water for biochemical oxygen demand (BOD) (BOD) up to thousands of mg/L, the aerobic treatment methods such as some biomembrance process and activated sludge process are difficult to tolerance, photosynthetic bacterium can be born, therefore photosynthetic bacterium is being with a wide range of applications aspect processing high concentrated organic wastewater.
Due to the concentrated difficulty of photosynthetic bacterium, concentrated cost is higher, therefore common photosynthetic bacteria preparation is unconcentrated liquid preparation in the market.Unconcentrated photosynthetic bacteria liquid volume is large, Packing Fee is high, transport and preserve inconvenience, finally causes photosynthetic bacteria liquid use cost to raise.Due to photosynthetic bacterium in the outstanding representation aspect water treatment, recently photosynthetic bacterium is also widely used in landscape water process field, but the quantity of the photosynthetic bacterium that landscape water will be used in processing is larger, therefore the high problem of use cost of the photosynthetic bacterium in landscape water is processed seems particularly outstanding.
Therefore the photosynthetic bacteria liquid that, how to provide a kind of convenience, economy, can be used for landscape water processing is those skilled in the art's technical problems urgently to be resolved hurrily.
Summary of the invention
The object of the present invention is to provide the simple and easy propagation method of a kind of photosynthetic bacterium for landscape water processing, existing for the too high problem of the photosynthetic bacteria liquid use cost of landscape water processing to solve.
To achieve these goals, technical scheme of the present invention is:
For the simple and easy propagation method of photosynthetic bacterium of landscape water processing, comprise the steps:
Step 1: a photosynthetic bacterium bacterial classification is provided;
Step 2: preparation bacteria culture medium;
Step 3: inoculate described photosynthetic bacterium bacterial classification according to predetermined amount in described bacteria culture medium, obtain photosynthetic bacterium inoculation liquid;
Step 4: described photosynthetic bacterium inoculation liquid is positioned under sunlight and cultivates predetermined number of days, obtain Fermentation by Photosynthetic Bacteria liquid.
Further, described step 4 comprises: described photosynthetic bacterium inoculation liquid is divided and installed in some clear vial, and described clear vial is positioned under sunlight.
Further, after described step 4, also comprise:
Step 5: the described Fermentation by Photosynthetic Bacteria liquid in described some clear vial is mixed and poured in a plastic tank, and stir;
Step 6: leave and take Fermentation by Photosynthetic Bacteria liquid described in the step 5 of 20%-25% as described photosynthetic bacterium bacterial classification;
Step 7: described in recirculation, step 2 is to step 6.
Further, in described step 7,1-2 described step 2 of recirculation is to step 6.
Further, in described step 3, in the time of 20 ℃ of continuous 3 daily mean temperature >, in described bacteria culture medium, access the described photosynthetic bacterium bacterial classification of 12%-17%; In the time of continuous 3 daily mean temperature≤20 ℃, in described bacteria culture medium, access the described photosynthetic bacterium bacterial classification of 18%-22%.
Further, described predetermined number of days is 2-7 days.
Further, described bacteria culture medium chief component is:
Further, in described step 2, after even each components dissolved of the described bacteria culture medium of composition, test potential of hydrogen, and adjust pH value to 7.0-7.4.
Further, described clear vial is Plastic Bottle.
Compared with prior art, tool has the following advantages and beneficial effect in the present invention:
1, the simple and easy propagation method of the photosynthetic bacterium for landscape water processing of the present invention, take commercialization photosynthetic bacteria preparation product as original strain, by economic bacteria culture medium and easy propagation method, the vacant lot that the landscape water body of processing at needs is other or other shine upon good place and cultivate.Photosynthetic bacteria liquid after cultivation can come into operation immediately at use scene, has omitted transportation cost, and its using dosage is consistent with the dosage of general merchandise photosynthetic bacterium.Adopt present method not only to reduce the transportation cost for the photosynthetic bacteria liquid of landscape water processing, also guaranteed the quality stability for the photosynthetic bacteria liquid of landscape water processing.
2, the simple and easy propagation method of the photosynthetic bacterium for landscape water processing of the present invention, the original strain adopting is commercialization photosynthetic bacteria preparation, by economic bacteria culture medium and easy cultural method, can facilitate, obtain economically can be used for the photosynthetic bacteria liquid of landscape water processing, and original commercialization photosynthetic bacteria preparation can Cyclic culture 2-3 time as bacterial classification, further effectively reduces the production cost for the photosynthetic bacteria liquid of landscape water processing.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail for the simple and easy propagation method of photosynthetic bacterium of landscape water processing.According to the following describes and claims, advantages and features of the invention will be clearer.
For the simple and easy propagation method of photosynthetic bacterium of landscape water processing, comprise the steps:
Step 1: a photosynthetic bacterium bacterial classification is provided.
Preferably, can directly buy from the market commercialization photosynthetic bacteria preparation as photosynthetic bacterium bacterial classification.
Step 2: preparation bacteria culture medium.According to the growth characteristic of photosynthetic bacterium, select suitable microbial culture based formulas, specifically, for the Financial cost that makes to cultivate for the photosynthetic bacteria liquid of landscape water processing is minimum, obtain best culture effect, bacteria culture medium chief component is:
Weigh desired substance by above-mentioned formula, dissolve and mix one by one, test potential of hydrogen also regulates pH value, is made into bacteria culture medium.Certainly, also can first be made into mother liquor, when use, add in proportion certain amount.Preferably, in order to guarantee the normal growth of photosynthetic bacterium, regulating the pH value of bacteria culture medium is 7.0-7.4.
Step 3: according to predetermined amount inoculation photosynthetic bacterium bacterial classification, obtain photosynthetic bacterium inoculation liquid in bacteria culture medium.After bacteria culture medium prepares, should inoculate immediately, cultivate and select suitable inoculum size according to photosynthetic bacterium productivity.Inoculum size refers to bacterial classification mother liquor amount and newly joins the volume ratio of inoculum.While selecting inoculum size, to guarantee the absolute predominance of photosynthetic bacterium in inoculum, in order to avoid affect ultimate capacity and quality that photosynthetic bacterium is cultivated.
Step 4: photosynthetic bacterium inoculation liquid is positioned under sunlight and cultivates predetermined number of days, obtain Fermentation by Photosynthetic Bacteria liquid.In photosynthetic bacterium culturing process, need to rock, effect is to help the photosynthetic bacterium floating that is deposited in bottom to obtain illumination, and assurance photosynthetic bacterium mycetocyte is well grown.
Specifically, first the photosynthetic bacterium bacterial classification of predetermined amount is inoculated in the bacteria culture medium of having prepared, obtain photosynthetic bacterium inoculation liquid, then photosynthetic bacterium inoculation liquid is divided and installed in some clear vial, and some clear vial are positioned under sunlight is Fermentation by Photosynthetic Bacteria liquid to obtain satisfactory photosynthetic bacteria liquid.Adopt the method for packing more first inoculated, can reduce aerial open-assembly time of photosynthetic bacterium bacterial classification, disposable inoculation is complete can prevent the secondary pollution that causes because repeatedly opening photosynthetic bacterium bacterial classification container.
Especially, in order to meet the growing environment of photosynthetic bacterium, in clear vial, postvaccinal photosynthetic bacterium cultivated days is because of the variation difference of temperature and illumination.Because the selection of inoculum size is also closely related with temperature in photosynthetic bacterium culturing process, therefore should be according to selecting suitable inoculum size when ground temperature.Preferably, in the time of 20 ℃ of continuous 3 daily mean temperature >, in bacteria culture medium, access the photosynthetic bacterium bacterial classification of 12%-17%; In the time of continuous 3 daily mean temperature≤20 ℃, in bacteria culture medium, access the photosynthetic bacterium bacterial classification of 18%-22%.After having inoculated, obtain photosynthetic bacterium inoculation liquid, photosynthetic bacterium inoculation liquid is divided and installed in some clear vial, and some clear vial bottle caps are covered tightly, be positioned under sunlight, or the other vacant lot of the landscape water body that need to process or other shine upon good place, to meet the illumination condition of photosynthetic bacterium.Certainly,, in order to guarantee mycetocyte floating and the fermentation homogeneity of photosynthetic bacterium, sooner or later at least respectively shake clear vial 1 time every day.
Conventionally, need to cultivate 2-7 days, photosynthetic bacterium inoculation liquid can fully fermenting.How to judge whether fully fermenting of solution of photosynthetic bacteria, can visual observations, when upper and lower color even in clear vial, significantly layering without comparison, it is also more pleasing that outward appearance seems.Certainly,, for judgement more accurately, also can adopt turbidometer or bacterial turbidity meter to detect.
Further, in conjunction with the own characteristic of photosynthetic bacterium, generally select the cultivation of transparent Plastic Bottle for photosynthetic bacterium.In order conveniently to draw materials and cost-saving, it is the Plastic Bottle of 5L that the clear vial in step 3 can be selected volume.
Step 5: the Fermentation by Photosynthetic Bacteria liquid in some clear vial is mixed and poured in a plastic tank, and stir.Especially, and density unevenness cause use in different for fear of the concentration of the Fermentation by Photosynthetic Bacteria liquid in different clear vial, the Fermentation by Photosynthetic Bacteria liquid of cultivating the different clear vial of same batch after predetermined number of days for example can be poured into, in the container (plastic tank that volume is 300L) of a fixed volume, and the Fermentation by Photosynthetic Bacteria liquid stirring after being mixed.Photosynthetic bacteria liquid now can be directly used in landscape water processing.
Step 6: leave and take the Fermentation by Photosynthetic Bacteria liquid of 20%-25% as photosynthetic bacterium bacterial classification.Specifically, in order to reduce production costs, in conjunction with the feature of photosynthetic bacterium self growth, can leave and take the Fermentation by Photosynthetic Bacteria liquid obtaining in step 5 as photosynthetic bacterium bacterial classification, productivity for follow-up photosynthetic bacterium is cultivated, and remaining can directly be put in landscape water body according to the usage quantity of commercialization photosynthetic bacteria preparation.
Step 7: recirculation step 2, to step 6, obtains Fermentation by Photosynthetic Bacteria liquid, whether up to standard Fermentation by Photosynthetic Bacteria liquid now determination methods be the same, repeats no more herein.
Specifically, in order further to reduce production costs, can recirculation repeatedly step 2 to step 6.But in order to guarantee the activity of Fermentation by Photosynthetic Bacteria liquid, preferred, 1-2 step 2 of recirculation, to step 6, need to be bought new photosynthetic bacteria preparation again as photosynthetic bacterium bacterial classification.
In sum, the simple and easy propagation method of the photosynthetic bacterium for landscape water processing that the present embodiment provides, has solved existing for the too high problem of the photosynthetic bacterium use cost of landscape water processing.The simple and easy propagation method of photosynthetic bacterium for landscape water processing of the present invention, take commercialization photosynthetic bacteria preparation product as original strain, by economic bacteria culture medium and easy propagation method, the vacant lot that the landscape water body of processing at needs is other or other shine upon good place and cultivate.Can facilitate, obtain economically can be used for the photosynthetic bacteria liquid of landscape water processing by this easy cultural method, and original commercialization photosynthetic bacteria preparation can Cyclic culture 2-3 time as bacterial classification, further effectively reduces the production cost for the photosynthetic bacteria liquid of landscape water processing.The photosynthetic bacteria liquid that cultivation obtains can come into operation immediately at use scene, has omitted transportation cost, and its using dosage is consistent with the dosage of general merchandise photosynthetic bacterium.Adopt present method not only to reduce the transportation cost for the photosynthetic bacteria liquid of landscape water processing, also guaranteed the quality stability for the photosynthetic bacteria liquid of landscape water processing.
Foregoing description is only the description to preferred embodiment of the present invention, not any restriction to the scope of the invention.Any change, modification that those skilled in the art does according to above-mentioned disclosure, all belong to the protection domain of claim.
Claims (9)
1. for the simple and easy propagation method of photosynthetic bacterium of landscape water processing, it is characterized in that, comprise the steps:
Step 1: a photosynthetic bacterium bacterial classification is provided;
Step 2: preparation bacteria culture medium;
Step 3: inoculate described photosynthetic bacterium bacterial classification according to predetermined amount in described bacteria culture medium, obtain photosynthetic bacterium inoculation liquid;
Step 4: described photosynthetic bacterium inoculation liquid is positioned under sunlight and cultivates predetermined number of days, obtain Fermentation by Photosynthetic Bacteria liquid.
2. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 1, is characterized in that, described step 4 comprises: described photosynthetic bacterium inoculation liquid is divided and installed in some clear vial, and described clear vial is positioned under sunlight.
3. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 2, is characterized in that, after described step 4, also comprises:
Step 5: the described Fermentation by Photosynthetic Bacteria liquid in described some clear vial is mixed and poured in a plastic tank, and stir;
Step 6: leave and take Fermentation by Photosynthetic Bacteria liquid described in the step 5 of 20%-25% as described photosynthetic bacterium bacterial classification;
Step 7: described in recirculation, step 2 is to step 6.
4. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 3, is characterized in that, in described step 7,1-2 described step 2 of recirculation is to step 6.
5. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 1 or 2, is characterized in that, in described step 3,
In the time of 20 ℃ of continuous 3 daily mean temperature >, in described bacteria culture medium, access the described photosynthetic bacterium bacterial classification of 12%-17%;
In the time of continuous 3 daily mean temperature≤20 ℃, in described bacteria culture medium, access the described photosynthetic bacterium bacterial classification of 18%-22%.
6. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 1 or 2, is characterized in that, described predetermined number of days is 2-7 days.
7. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 1, is characterized in that, described bacteria culture medium chief component is:
8. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 7, is characterized in that, in described step 2, and after even each components dissolved of the described bacteria culture medium of composition, test potential of hydrogen, and adjust pH value to 7.0-7.4.
9. the simple and easy propagation method of the photosynthetic bacterium for landscape water processing as claimed in claim 2, is characterized in that, described clear vial is Plastic Bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410122552.XA CN103865858A (en) | 2014-03-28 | 2014-03-28 | Simple spread cultivation method for photosynthetic bacteria for treating landscape water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410122552.XA CN103865858A (en) | 2014-03-28 | 2014-03-28 | Simple spread cultivation method for photosynthetic bacteria for treating landscape water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103865858A true CN103865858A (en) | 2014-06-18 |
Family
ID=50904870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410122552.XA Pending CN103865858A (en) | 2014-03-28 | 2014-03-28 | Simple spread cultivation method for photosynthetic bacteria for treating landscape water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103865858A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105219680A (en) * | 2015-11-02 | 2016-01-06 | 湖北中化东方肥料有限公司 | A kind of preparation method of photosynthetic bacterium microbial inoculum and application thereof |
| CN106365322A (en) * | 2016-05-25 | 2017-02-01 | 路域生态工程有限公司 | Method for highly efficiently treating black stinking water |
| CN108484237A (en) * | 2018-04-28 | 2018-09-04 | 沈阳师范大学 | A kind of preparation method and application of agricultural photosynthetic bacteria microorganism fertilizer |
| CN110771426A (en) * | 2019-10-21 | 2020-02-11 | 郭红伟 | Stable culture method of phellinus igniarius strains |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597579A (en) * | 2009-05-14 | 2009-12-09 | 佛山市顺德区宏隆生物科技有限公司 | A kind of production method of agricultural photosynthetic bacteria preparation |
| CN101698539A (en) * | 2009-10-13 | 2010-04-28 | 王颖 | Microecological agent for purifying water and preparation method thereof |
-
2014
- 2014-03-28 CN CN201410122552.XA patent/CN103865858A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101597579A (en) * | 2009-05-14 | 2009-12-09 | 佛山市顺德区宏隆生物科技有限公司 | A kind of production method of agricultural photosynthetic bacteria preparation |
| CN101698539A (en) * | 2009-10-13 | 2010-04-28 | 王颖 | Microecological agent for purifying water and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 孙军德 等: "沼泽红假单胞菌培养条件研究", 《沈阳农业大学学报》 * |
| 宜春强微生物科技有限公司: "光合细菌的培养操作教程", 《HTTP://WWW.HL99CN.COM/SHOW.ASP?NEWSID=2380》 * |
| 宜春强微生物科技有限公司: "光合细菌的培养操作教程", 《HTTP://WWW.HL99CN.COM/SHOW.ASP?NEWSID=2380》, 27 May 2013 (2013-05-27) * |
| 陈秀为: "《光合菌在农业中的应用》", 31 March 1998 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105219680A (en) * | 2015-11-02 | 2016-01-06 | 湖北中化东方肥料有限公司 | A kind of preparation method of photosynthetic bacterium microbial inoculum and application thereof |
| CN106365322A (en) * | 2016-05-25 | 2017-02-01 | 路域生态工程有限公司 | Method for highly efficiently treating black stinking water |
| CN108484237A (en) * | 2018-04-28 | 2018-09-04 | 沈阳师范大学 | A kind of preparation method and application of agricultural photosynthetic bacteria microorganism fertilizer |
| CN110771426A (en) * | 2019-10-21 | 2020-02-11 | 郭红伟 | Stable culture method of phellinus igniarius strains |
| CN110771426B (en) * | 2019-10-21 | 2021-08-27 | 郭红伟 | Stable culture method of phellinus igniarius strains |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Removal of nutrients from undiluted anaerobically treated piggery wastewater by improved microalgae | |
| Meng et al. | Effects of dissolved oxygen concentration on photosynthetic bacteria wastewater treatment: pollutants removal, cell growth and pigments production | |
| CN100410362C (en) | Method for culturing chlorella with high-density and high-quality | |
| Fallowfield et al. | The photosynthetic treatment of pig slurry in temperate climatic conditions: a pilot-plant study | |
| CN101407774B (en) | Preparation technique of photosynthetic bacteria preparation | |
| CN103484370B (en) | A kind of method utilizing farm animal excrement waste water to cultivate spirulina | |
| CN102212491A (en) | Simple culture method of photosynthetic bacteria with high cell concentration | |
| CN103865858A (en) | Simple spread cultivation method for photosynthetic bacteria for treating landscape water | |
| CN101407761B (en) | Liquid inocula composed of yeast fused strain, Geotrichum candidum Link and Rhizopus, and preparation and use thereof | |
| Lu et al. | Production of photosynthetic bacteria using organic wastewater in photobioreactors in lieu of a culture medium in fermenters: From lab to pilot scale | |
| Barzee et al. | Pilot microalgae cultivation using food waste digestate with minimal resource inputs | |
| CN105505843B (en) | One plant of Photosynthetic bacterium strain, the Liquid Fertilizer containing the bacterial strain and preparation method, application | |
| CN106745814A (en) | Compound improver of water quality for aquaculture and preparation method thereof | |
| CN107384832B (en) | Method for culturing nitrogen-fixing blue algae in large quantity at low cost | |
| CN105886437A (en) | Composition or composite bacterium agent for controlling water body pollution | |
| CN101974473A (en) | Method for massively producing photosynthetic bacteria under natural ecological condition | |
| CN103966103A (en) | Culture medium and culture method for culturing haematococcus pluvialis by using brewery wastewater | |
| CN109797106A (en) | A kind of novel two stages autotrophy-is photosynthetic and supports the method for improving chlorella lipid | |
| CN103588304B (en) | A kind of cultivating pool Aquatic Microbiota group that is used for regulates and controls carbon source and application thereof | |
| CN103382491B (en) | Preparation method of citric acid | |
| CN106754543A (en) | A kind of microorganism formulation for sludge anaerobic fermentation | |
| CN106754385A (en) | A kind of utilization blue-green alga bloom is the method for raw material cultivation Chlorella phytoplankton | |
| CN112794774A (en) | Composite organic liquid biological bacterial fertilizer applied to grape planting and preparation method thereof | |
| CN204824659U (en) | Organic microbial manure apparatus for producing | |
| Krustok et al. | Cultivation of indigenous algae for increased biogas production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140618 |