CN103860653B - The hypertensive compound blood pressure reducing capsule of treatment-refractory and preparation and application - Google Patents
The hypertensive compound blood pressure reducing capsule of treatment-refractory and preparation and application Download PDFInfo
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Abstract
A kind of hypertensive compound blood pressure reducing capsule of treatment-refractory and preparation and application, it is a kind of liver-yang hyperactivity for treatment vascular hypertension, the preparation technology of the compound blood pressure reducing capsule of knot in the water stasis of blood, the alcohol extract of the dregs of a decoction that produced after Extraction Process of Volatile Oil by mother chrysanthemum, bluish dogbane, the root bark of white mulberry, sophora flower, the mixed liquor obtaining after the filtrate that the combination water extract of selfheal adds mother chrysanthemum to extract to filter in volatile oil process the dregs of a decoction to produce, by alcohol extract and mixed liquor respectively through concentrated, dry, two kinds of dried cream powders that obtain after pulverizing mix and add supplementary product starch granulation, the volatile oil of spraying again mother chrysanthemum extraction mixes composition. the compound blood pressure reducing capsule of making by preparation technology of the present invention can be assisted western medicine refractory hypertension, plays adjusting blood pressure, protection hypertensive cerebral vascular remodeling and prevent the effect of the target organ damages such as kidney.
Description
Technical field
The present invention relates to a kind of Chinese patent drug and preparation thereof and application, relate in particular to the hypertensive compound blood pressure reducing capsule of a kind of treatment-refractory and preparation and application.
Background technology
Refractory hypertension (RH) is the high blood pressure of pendulum a kind of specific type in face of all clinicians, and normal with serious target organ damage, patient the danger of heart failure, cerebral apoplexy, myocardial infarction, renal insufficiency occurs and increases, and prognosis is poor. Find that according to data statistics refractory hypertension accounts for 12%～15% of clinical hypertension incidence, and follow the aging of this population and increasing of the fat incidence of crowd, refractory hypertension has the trend raising gradually. Therefore, how to make refractory hypertension patient's blood pressure level up to standard, the conditions of patients tool that is improved is had very important significance.
Refractory hypertension refers on the basis of the pattern of making the life better, enough three kinds and above antihypertensive drugs (must comprise diuretics) are used, blood pressure people can not person up to standard (≤140/90mmHg), or diabetes, nephrotic's blood pressure > 130/80mmHg, be called refractory hypertension or intractable hypertension. Western medicine refractory hypertension is difficult to obtain further curative effect.
The traditional Chinese medical science has unique dialectical understanding for refractory hypertension, theory of traditional Chinese medical science belongs to refractory hypertension the categories such as " headache ", " dizzy ", " liver-yang ", " liver wind ", " apoplexy ", and thinks that the generation of this disease is main with disorder of emotion, eating and drinking without temperance, internal injury is deficient, native endowment is relevant. Traditional Chinese Medical on Treating Hypertension is with a long history, has also obtained clear and definite clinical efficacy. By the combination of Chinese tradiational and Western medicine, complement each other each other, be the new approaches for the treatment of refractory hypertension.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, and compound blood pressure reducing capsule and preparation and the application of the refractory hypertension of tying in a kind of liver-yang hyperactivity for treatment vascular hypertension, the water stasis of blood are provided.
For solving the problems of the technologies described above, the technical scheme that the present invention proposes is:
One can be used for the hypertensive compound blood pressure reducing capsule of treatment-refractory, and the filler of described compound blood pressure reducing capsule is mainly that after being mixed and made into a material and being sprayed into volatile oil a again by dried cream powder I, dried cream powder II and auxiliary material, mixture forms;
Described dried cream powder I is traditional Chinese medicine ingredients A gained dregs of a decoction after Extraction Process of Volatile Oil, then obtains after alcohol extracting, concentrate drying;
The water extraction liquid that described dried cream powder II is traditional Chinese medicine ingredients B merges the filtrate of filtering the dregs of a decoction generation when above-mentioned traditional Chinese medicine ingredients A extracts volatile oil, then obtains after concentrated, dry;
Described traditional Chinese medicine ingredients A is mother chrysanthemum 20 ~ 50 mass parts;
Described traditional Chinese medicine ingredients B comprises: bluish dogbane 30 ~ 50 mass parts, sophora flower 10 ~ 25 mass parts, the root bark of white mulberry 10 ~ 15 mass parts, selfheal 10 ~ 25 mass parts;
Described auxiliary material is starch.
The mass ratio of described dried cream powder and starch is 4:1.
Above-mentioned compound blood pressure reducing capsule, its preparation method is selected the raw material constituent that comprises following mass fraction proportioning:
30 ~ 50 parts of bluish dogbanes
20 ~ 50 parts of mother chrysanthemums
10 ~ 15 parts of the root barks of white mulberry
10 ~ 25 parts, sophora flower
10 ~ 25 parts of selfheals
5 ~ 15 parts of starch
Based on above-mentioned raw materials, described preparation method comprises the following steps:
(1) extract volatile oil: by traditional Chinese medicine ingredients A pretreatment, through the extraction process of volatile oil, collect volatile oil a and residual dregs of a decoction b, filtrate c with for subsequent use;
(2) alcohol extracting: carry out alcohol extracting to traditional Chinese medicine ingredients A residual dregs of a decoction b after Extraction Process of Volatile Oil, get alcohol extract, concentrate, be dried, pulverize, obtain dried cream powder I;
(3) water extraction: traditional Chinese medicine ingredients B is carried out to water extraction, after water extraction liquid is mixed with filtrate c, concentrate, be dried, pulverize, obtain dried cream powder II;
(4) mix: the dried cream powder I obtaining of step (2) is evenly mixed with the dried cream powder II that step (3) obtains, then add auxiliary material, granulation, dry, spray into volatile oil a, mix, obtain capsule filling;
(5) make finished product: the capsule filling that step (4) is obtained incapsulates, packaging, obtains finished product compound blood pressure reducing capsule.
In preparation method of the present invention, the extraction process of described volatile oil, for mother chrysanthemum is added after a small amount of water soaking 0.5 ~ 1.5h, continues to add adding water to 4 ~ 10 times of recipe quantities, with extraction by steam distillation volatile oil, and extraction time 3 ~ 6h.
In preparation method of the present invention, preferred, described alcohol extracting comprises three alcohol extractings, alcohol extracting for the first time: after Extraction Process of Volatile Oil in residual dregs of a decoction b, add 85% ethanol of 8 ~ 12 times of recipe quantities to mother chrysanthemum, extract taking ethanol as auxiliary agent, extraction time 1 ~ 2.5h, extract is for subsequent use; Alcohol extracting for the second time: the dregs of a decoction that produce with alcohol extracting for the first time add 85% ethanol of 6 ~ 10 times of recipe quantities, carry out second extraction taking ethanol as auxiliary agent, extraction time 0.5 ~ 2h, extract is for subsequent use; Alcohol extracting for the third time: the dregs of a decoction that produce with alcohol extracting for the second time add 85% ethanol of 4 ~ 10 times of recipe quantities, carries out three times and extracts taking ethanol as auxiliary agent, extraction time 0.5 ~ 2h, and extract is for subsequent use, then will each extract mixing, the dregs of a decoction discard.
In preparation method of the present invention, preferred, described water extraction comprises water extraction, for the first time water extraction three times: add after a small amount of water soaking 0 ~ 1.5h to traditional Chinese medicine ingredients B, continue to add adding water to 6 ~ 15 times of recipe quantities and extract, and extraction time 1 ~ 2h, extract is for subsequent use; Water extraction for the second time: add the water of 5 ~ 12 times of recipe quantities with the dregs of a decoction that water extraction produces for the first time, carry out second extraction, extraction time 0.5 ~ 1.5h, extract is for subsequent use; Water extraction for the third time: add the water of 5 ~ 12 times of recipe quantities with the dregs of a decoction that water extraction produces for the second time, carry out three times and extract, extraction time 0.5 ~ 1.5h, extract is for subsequent use, then each extract is mixed, and the dregs of a decoction discard.
In preparation method of the present invention, described mother chrysanthemum alcohol extracting extract carries out reduced pressure concentration in 50 ~ 70 DEG C, is concentrated to relative density as for 1.3 ~ 1.35, is then placed in 55 ~ 65 DEG C of vacuum, carries out vacuum drying.
In preparation method of the present invention, the extract of described water extraction carries out reduced pressure concentration in 60 ~ 80 DEG C, is concentrated to relative density as for 1.3 ~ 1.35, is then placed in 65 ~ 75 DEG C of vacuum, carries out vacuum drying.
In preparation method of the present invention, the described mixing granulation of step (4) adopts wet granulation, after adding supplementary product starch, add ethanol to granulate again, the particle of making is crossed 60 ~ 100 mesh sieves, then be placed in not higher than carrying out the dry of particle under the environment of 50 DEG C, the volatile oil a obtaining with step (1) mixes.
The present invention also comprises that compound blood pressure reducing capsule that above-mentioned compound blood pressure reducing capsule or above-mentioned preparation method make is in the application for the preparation of in treatment refractory hypertension medicine.
In the present invention, described compound blood pressure reducing capsule is adjuvant, is applied to treatment-refractory hypertension, and not only itself just has good voltage regulation result, and western medicine of depressurization fiting effect is more remarkable.
In the present invention, in raw material, the pharmacology of each component is:
Bluish dogbane: micro-hardship, cool. Return Liver Channel. Flat liver is calmed the nerves, effect of clearing away heat and promoting diuresis. Modern pharmacological research thinks that can expand peripheral vessels has hypotensive effect with suppressing adrenaline, has diuresis.
Mother chrysanthemum: pungent, sweet, bitter, be slightly cold. Return lung, Liver Channel. Dispelling wind and heat from the body, flat liver improving eyesight, clearing heat and detoxicating effect. Modern pharmacological research finds that there is calmness, refrigeration function; Can expand peripheral vessels and step-down; There is coronary artery dilator and increase coronary blood flow effect; Decoction soak has obvious effect to local inflammation.
The root bark of white mulberry: sweet, cold. Return lung channel. Removing heat from lung and relieving asthma, effect of inducing diuresis for removing edema. Pharmacological research finds that there is diuresis, can take out of compared with polychloride, and have calmness and hypotensive effect.
Sophora flower: bitter, be slightly cold. Return liver, large intestine channel. Cooling blood and hemostasis, effect of clearing liver-fire. Pharmacological research finds that there is and improves capillary fragility and hypotensive activity, and experimental atherosclerosis disease is had to preventive and therapeutic action.
Selfheal: bitter, acrid, cold. Return liver, gallbladder channel. Clear liver and improve vision, effect of mass dissipating and swelling eliminating. For the dizzy common medicine of headache for the treatment of irascibility order disease and liver-yang hyperactivity. Pharmacology: inorganic salts part has hypotensive effect; Decoction can reduce blood pressure and mitigation symptoms clinically; There is obvious diuresis.
Compared with prior art, the invention has the advantages that: in the present invention, a few herbs effect concentrates on flat liver, heat-clearing, Li Shui, for refractory hypertension liver-yang hyperactivity, the symptom of knot in the water stasis of blood. The expansible blood vessel of bluish dogbane and mother chrysanthemum reduces blood pressure; Bluish dogbane and selfheal etc. have obvious diuresis; Mother chrysanthemum, the root bark of white mulberry, sophora flower improve the effect of the performance protection blood vessel structures such as blood vessel elasticity by inflammation-inhibiting. Entirety can be assisted western medicine refractory hypertension, plays adjusting blood pressure, protects hypertensive cerebral vascular remodeling and prevents the effect of the target organ damages such as kidney.
Detailed description of the invention
Below in conjunction with concrete preferred embodiment, the invention will be further described, but protection domain not thereby limiting the invention.
Embodiment mono-
Former medicine is prepared: measure bluish dogbane 40g, mother chrysanthemum 20g, root bark of white mulberry 10g, sophora flower 10g, selfheal 10g, starch supplementary material 10g by prescription.
Preparation method embodiment mono-
(1) extract volatile oil: 20g mother chrysanthemum is added after a small amount of water soaking 0.5h, continue to add 100g water, with extraction by steam distillation volatile oil a, extraction time 3.5h;
Collect dregs of a decoction b, the filtrate c of volatile oil a and filtration residue with for subsequent use;
(2) alcohol extracting: get the dregs of a decoction b that step (1) generates and carry out three alcohol extractings: alcohol extracting for the first time: to mother chrysanthemum after Extraction Process of Volatile Oil in residual dregs of a decoction b, add 85% the ethanol of 180g, extract taking ethanol as auxiliary agent, extraction time 1.5h, extract is for subsequent use, alcohol extracting for the second time: the dregs of a decoction that alcohol extracting for the first time produces add 85% the ethanol of 140g, carry out second extraction taking ethanol as auxiliary agent, extraction time 1h, extract is for subsequent use, alcohol extracting for the third time: the dregs of a decoction that alcohol extracting for the second time produces add 85% the ethanol of 100g, carry out three times extracts taking ethanol as auxiliary agent, extraction time 2h, extract is for subsequent use, then by each alcohol extract mixing for standby use, the dregs of a decoction discard, alcohol extract is carried out to reduced pressure concentration in 50 DEG C, be concentrated to relative density as for 1.3, then be placed in 55 DEG C of vacuum, carry out vacuum drying, pulverize again, obtain dried cream powder I,
(3) water extraction: by bluish dogbane 40g, root bark of white mulberry 10g, sophora flower 10g, after selfheal 10g mixes, composition traditional Chinese medicine ingredients B carries out water extraction three times, water extraction for the first time: add after a small amount of water soaking 0.5h to traditional Chinese medicine ingredients B, continuing to add 560g water extracts, extraction time 1h, extract is for subsequent use, water extraction for the second time: the dregs of a decoction that water extraction produces for the first time add 420g water, carry out second extraction, extraction time 0.5h, extract is for subsequent use, water extraction for the third time: the dregs of a decoction that water extraction produces for the second time add 350g water, carrying out three times extracts, extraction time 1h, extract is for subsequent use, then each water extraction liquid is mixed, the dregs of a decoction discard, after the filtrate c that water extraction liquid is generated with step (1) mixes, mixed liquor is carried out to reduced pressure concentration in 60 DEG C, be concentrated to relative density as for 1.3, be then placed in 65 DEG C of vacuum, carry out vacuum drying, then pulverize, obtain dried cream powder II,
(4) mix: the dried cream powder I obtaining of step (2) is evenly mixed with the dried cream powder II that step (3) obtains, add again 10g supplementary product starch, add 95% ethanol granulation, cross 60 object sieves, then be placed in and under the environment of 40 DEG C, carry out the dry of particle, spray into again the volatile oil a that step (1) generates, mix, obtain capsule filling;
(5) make finished product: the capsule filling that step (4) is obtained incapsulates, packaging, obtains finished product compound blood pressure reducing capsule.
Embodiment bis-
Former medicine is prepared: measure bluish dogbane 45g by prescription, mother chrysanthemum 22g, root bark of white mulberry 12g, sophora flower 12g, selfheal 12g, starch supplementary material 15g
Preparation method embodiment bis-
(1) extract volatile oil: 22g mother chrysanthemum is added after a small amount of water soaking 1h, continue to add 132g water, with extraction by steam distillation volatile oil a, extraction time 4.5h;
Collect dregs of a decoction b, the filtrate c of volatile oil a and filtration residue with for subsequent use;
(2) alcohol extracting: get the dregs of a decoction b that step (1) generates and carry out three alcohol extractings: alcohol extracting for the first time: to mother chrysanthemum after Extraction Process of Volatile Oil in residual dregs of a decoction b, add 85% the ethanol of 220g, extract taking ethanol as auxiliary agent, extraction time 2h, extract is for subsequent use, alcohol extracting for the second time: the dregs of a decoction that alcohol extracting for the first time produces add 85% the ethanol of 176g, carry out second extraction taking ethanol as auxiliary agent, extraction time 1.5h, extract is for subsequent use, alcohol extracting for the third time: the dregs of a decoction that alcohol extracting for the second time produces add 85% the ethanol of 154g, carry out three times extracts taking ethanol as auxiliary agent, extraction time 1.5h, extract is for subsequent use, then by each alcohol extract mixing for standby use, the dregs of a decoction discard, alcohol extract is carried out to reduced pressure concentration in 60 DEG C, be concentrated to relative density as for 1.3, then be placed in 60 DEG C of vacuum, carry out vacuum drying, pulverize again, obtain dried cream powder I,
(3) water extraction: by bluish dogbane 45g, root bark of white mulberry 12g, sophora flower 12g, after selfheal 12g mixes, composition traditional Chinese medicine ingredients B carries out water extraction three times, water extraction for the first time: add after a small amount of water soaking 1h to traditional Chinese medicine ingredients B, continuing to add 810g water extracts, extraction time 1h, extract is for subsequent use, water extraction for the second time: the dregs of a decoction that water extraction produces for the first time add 640g water, carry out second extraction, extraction time 1.5h, extract is for subsequent use, water extraction for the third time: the dregs of a decoction that water extraction produces for the second time add 560g water, carrying out three times extracts, extraction time 1h, extract is for subsequent use, then each water extraction liquid is mixed, the dregs of a decoction discard, after the filtrate c that water extraction liquid is generated with step (1) mixes, mixed liquor is carried out to reduced pressure concentration in 70 DEG C, be concentrated to relative density as for 1.3, be then placed in 70 DEG C of vacuum, carry out vacuum drying, then pulverize, obtain dried cream powder II,
(4) mix: the dried cream powder I obtaining of step (2) is evenly mixed with the dried cream powder II that step (3) obtains, add again 15g supplementary product starch, add 95% ethanol granulation, cross 80 object sieves, then be placed in and under the environment of 45 DEG C, carry out the dry of particle, spray into again the volatile oil a that step (1) generates, mix, obtain capsule filling;
(5) make finished product: the capsule filling that step (4) is obtained incapsulates, packaging, obtains finished product compound blood pressure reducing capsule.
Embodiment tri-
Former medicine is prepared: measure bluish dogbane 50g by prescription, 25 parts of g of mother chrysanthemum, root bark of white mulberry 15g, sophora flower 15g, selfheal 15g, starch supplementary material 20g;
Preparation method embodiment tri-
(1) extract volatile oil: 25g mother chrysanthemum is added after a small amount of water soaking 1.5h, continue to add 225g water, with extraction by steam distillation volatile oil a, extraction time 5.5h;
Collect dregs of a decoction b, the filtrate c of volatile oil a and filtration residue with for subsequent use;
(2) alcohol extracting: get the dregs of a decoction b that step (1) generates and carry out three alcohol extractings: alcohol extracting for the first time: to mother chrysanthemum residual dregs of a decoction after Extraction Process of Volatile OilbIn, add 85% the ethanol of 275g, extract taking ethanol as auxiliary agent, extraction time 2.5h, extract is for subsequent use, alcohol extracting for the second time: the dregs of a decoction that alcohol extracting for the first time produces add 85% the ethanol of 225g, carry out second extraction taking ethanol as auxiliary agent, extraction time 1.5h, extract is for subsequent use, alcohol extracting for the third time: the dregs of a decoction that alcohol extracting for the second time produces add 85% the ethanol of 175g, carry out three times extracts taking ethanol as auxiliary agent, extraction time 2h, extract is for subsequent use, then by each alcohol extract mixing for standby use, the dregs of a decoction discard, alcohol extract is carried out to reduced pressure concentration in 70 DEG C, be concentrated to relative density as for 1.3, then be placed in 55 DEG C of vacuum, carry out vacuum drying, pulverize again, obtain dried cream powder I,
(3) water extraction: by bluish dogbane 50g, root bark of white mulberry 15g, sophora flower 15g, after selfheal 15g mixes, composition traditional Chinese medicine ingredients B carries out water extraction three times, water extraction for the first time: add after a small amount of water soaking 1.5h to traditional Chinese medicine ingredients B, continuing to add 1140g water extracts, extraction time 2h, extract is for subsequent use, water extraction for the second time: the dregs of a decoction that water extraction produces for the first time add 900g water, carry out second extraction, extraction time 1.5h, extract is for subsequent use, water extraction for the third time: the dregs of a decoction that water extraction produces for the second time add 700g water, carrying out three times extracts, extraction time 1.5h, extract is for subsequent use, then each water extraction liquid is mixed, the dregs of a decoction discard, after the filtrate c that water extraction liquid is generated with step (1) mixes, mixed liquor is carried out to reduced pressure concentration in 80 DEG C, be concentrated to relative density as for 1.3, be then placed in 75 DEG C of vacuum, carry out vacuum drying, then pulverize, obtain dried cream powder II,
(4) mix: the dried cream powder I obtaining of step (2) is evenly mixed with the dried cream powder II that step (3) obtains, add again 20g supplementary product starch, add 95% ethanol granulation, cross 100 object sieves, then be placed in and under the environment of 50 DEG C, carry out the dry of particle, spray into again the volatile oil a that step (1) generates, mix, obtain capsule filling;
(5) make finished product: the capsule filling that step (4) is obtained incapsulates, packaging, obtains finished product compound blood pressure reducing capsule.
Experimental verification of the present invention
The present invention is at spontaneous hypertensive rat (SHR) model, per os gives calcium channel antagonistic Amlodipine or Amlodipine share compound blood pressure reducing capsule, observes the effect of medicine to rat blood pressure, renal function, cardiac structure, sustainer and superior mesenteric artery vascular remodeling.
Test method: adopt 45 of the spontaneous hypertensive rats in 16 weeks age, be divided at random model group, amlodipine in treatment group and Amlodipine and add Herbal compound capsule low dosage, middle dosage treatment group and high-dose therapy group, treat 8 weeks continuously by gastric infusion. Within every 3 days, detect the variation of rat systolic pressure, diastolic pressure, mean arterial pressure; Blood drawing detects the indexs such as creatinine, urea nitrogen before administration and after administration; After administration finishes, dissect and core dirtyly, take and calculate cardiac weight, left ventricular mass, heart/body weight ratio, left ventricle/body weight ratio etc., separately core and dirtyly fix with aorta pectoralis, the conventional formaldehyde of superior mesenteric artery, FFPE, carries out tissue pathology checking.
Dosage:
Press Amlodipine 100mg/kg each, administration every day 2 times group as a comparison;
The compound blood pressure reducing capsule of making by preparation method described in compound blood pressure reducing capsule described in embodiment mono-, presses 50mg/kg to animals administer each, coordinates Amlodipine 100mg/kg each, and administration every day 2 times is as low dose group;
The compound blood pressure reducing capsule of making by preparation method described in compound blood pressure reducing capsule described in embodiment bis-, presses 100mg/kg to animals administer each, coordinates Amlodipine 100mg/kg each, dosage group in 2 conducts of administration every day;
The compound blood pressure reducing capsule of making by preparation method described in compound blood pressure reducing capsule described in embodiment tri-, presses 200mg/kg to animals administer each, coordinates Amlodipine 100mg/kg each, and administration every day 2 times is as high dose group.
Result:
1, the impact on blood pressure. Before treatment group, respectively organize rat blood pressure without significant difference. Treat after 8 weeks, amlodipine in treatment group obviously reduces spontaneous hypertensive rat systolic pressure, diastolic pressure and mean arterial pressure. Compared with amlodipine in treatment group, it is more remarkable that in Amlodipine+compound blood pressure reducing capsule low dose therapy group, Amlodipine+compound blood pressure reducing capsule, dosage treatment group and Amlodipine+compound blood pressure reducing capsule in high dose treatment group reduce the effect of systolic pressure, and Amlodipine+compound blood pressure reducing capsule in high dose reduces diastolic pressure effect and is significantly better than amlodipine in treatment group. Between group, relatively there is significant difference.
Table 1. medicine treatment SHR rat blood pressure level after 8 weeks
All numerical value represents with mean ± standard deviation. * P < 0.05vsSHR, #P < 0.05vs amlodipine in treatment group
2, the impact on renal function. After treatment; compared with SHR group; amlodipine in treatment group serum creatinine, urea nitrogen levels are without significant change; and in Amlodipine+compound blood pressure reducing capsule low dose therapy group, Amlodipine+compound blood pressure reducing capsule, dosage treatment group and Amlodipine+compound blood pressure reducing capsule in high dose treatment group rat serum creatinine (Scr), urea nitrogen (BUN) level significantly reduce, and point out it to have protective effect to renal function.
Table 2. medicine is treated the impact on SHR rat serum creatinine, urea nitrogen
All numerical value represents with mean ± standard deviation. * P < 0.05vsSHR
3, the impact on myocardial structural. Compared with normal group, SHR rat weighs whole-heartedly, left ventricle is heavy, when left ventricle weight/weight ratio conspicuousness increase (P < 0.01) of heavy/body weight whole-heartedly. Amlodipine in treatment group is without significant change, and in Amlodipine+compound blood pressure reducing capsule low dose therapy group, Amlodipine+compound blood pressure reducing capsule dosage treatment group and Amlodipine+compound blood pressure reducing capsule in high dose treatment group rat whole-heartedly heavy, left ventricle is heavy, whole-heartedly heavy/body weight when left ventricle weight/weight ratio significantly reduce. Histotomy and HE dyeing show, rats in normal control group cardiac muscle fibre marshalling, and border is complete, and space between cells is without congested, inflammatory cell infiltration. SHR rat heart muscle is generally loose, cardiac muscle cell's arrangement disorder. Amlodipine+compound blood pressure reducing capsule (basic, normal, high dosage) treatment suppresses cardiac myocyte hypertrophy, alleviates morphological change. Results suggest Amlodipine+compound blood pressure reducing capsule drug combination has protective effect to cardiac muscle.
4, the impact on aorta pectoralis and superior mesenteric artery reconstruct. Compared with normal group, in SHR group aorta pectoralis, superior mesenteric artery, film smooth muscle cell proliferation and vascular wall significantly thicken. Amlodipine in treatment group is without significant change, and Amlodipine+compound blood pressure reducing capsule (basic, normal, high dosage) treatment significantly suppresses film in SHR rat chest aorta, superior mesenteric artery and thickens, and alleviates morphological change. Results suggest Amlodipine+compound blood pressure reducing capsule drug combination has protective effect to cardiac muscle.
Conclusion: the compound blood pressure reducing capsule that the present invention prepares can effectively be controlled SHR rat blood pressure, protection renal function, improves myocardial structural and vascular remodeling, coordinates Amlodipine better effects if. Change other western medicine of depressurization effects consistent.
Claims (10)
1. the hypertensive compound blood pressure reducing capsule of treatment-refractory, is characterized in that the filling of described compound blood pressure reducing capsuleAfter owner will be mixed and made into a material and be sprayed into volatile oil a again by dried cream powder I, dried cream powder II and auxiliary material, mixture forms;
Described dried cream powder I is traditional Chinese medicine ingredients A gained dregs of a decoction after volatile oil a extraction process, then obtains after alcohol extracting, concentrate drying;
The water extraction liquid that described dried cream powder II is traditional Chinese medicine ingredients B filters dregs of a decoction generation while merging above-mentioned traditional Chinese medicine ingredients A extraction volatile oil aFiltrate, then obtain after concentrated, dry;
Described volatile oil a is after traditional Chinese medicine ingredients A soaks, and continues to add water, obtains with extraction by steam distillation;
Described traditional Chinese medicine ingredients A is mother chrysanthemum 20～50 weight portions;
Described traditional Chinese medicine ingredients B is: bluish dogbane 30～50 mass parts, sophora flower 10～25 mass parts, the root bark of white mulberry 10～15 mass parts,Selfheal 10～25 mass parts;
Described auxiliary material is starch.
2. a preparation method for the hypertensive compound blood pressure reducing capsule of treatment-refractory, is characterized in that, by following mass partsThe raw material of number proportioning is made:
Based on above-mentioned raw materials, described preparation method comprises the following steps:
(1) extract volatile oil a: by described mother chrysanthemum pretreatment, through the extraction process of volatile oil a, collect volatile oil a and mistakeFilter residual dregs of a decoction b, filtrate c with for subsequent use; The extraction process of described volatile oil a, for after mother chrysanthemum is soaked, continues to add water,With extraction by steam distillation volatile oil a;
(2) alcohol extracting: get the dregs of a decoction b that step (1) generates and carry out alcohol extracting, get alcohol extract, concentrate, be dried, pulverize,Obtain dried cream powder I;
(3) water extraction: traditional Chinese medicine ingredients B is carried out to water extraction, after the filtrate c that water intaking extract generates with step (1) mixes, carry outConcentrated, dry, pulverizing, obtain dried cream powder II; Described traditional Chinese medicine ingredients B is bluish dogbane 30～50 mass parts, sophora flower 10～25 qualityPart, the root bark of white mulberry 10～15 mass parts, selfheal 10～25 mass parts;
(4) mix: the dried cream powder I obtaining of step (2) is evenly mixed with the dried cream powder II that step (3) obtains, then addSupplementary product starch, granulation, dry, spray into the volatile oil a that step (1) generates, mix, obtain capsule filling;
(5) make finished product: the capsule filling that step (4) is obtained incapsulates, packaging, obtains finished product compound blood pressure reducingCapsule.
3. preparation method according to claim 2, is characterized in that, the extraction process of described volatile oil a is for to add mother chrysanthemumAdd after a small amount of water soaking 0.5～1.5h, continue to add adding water to 4～14 times of recipe quantities, with extraction by steam distillation volatile oil a, carryGet time 3～6h.
4. preparation method according to claim 2, is characterized in that, described alcohol extracting comprises three alcohol extractings; Alcohol extracting for the first time:In described dregs of a decoction b, add 85% ethanol of 8～12 times of recipe quantities, extract taking ethanol as auxiliary agent, extraction time 1～2.5h, extract is for subsequent use; Alcohol extracting for the second time: the dregs of a decoction that produce with alcohol extracting for the first time add 85% second of 6～10 times of recipe quantitiesAlcohol, carries out second extraction taking ethanol as auxiliary agent, extraction time 0.5～2h, and extract is for subsequent use; Alcohol extracting for the third time: with alcohol for the second timeThe dregs of a decoction of proposing generation add 85% ethanol of 4～10 times of recipe quantities, carry out three times and extract, extraction time taking ethanol as auxiliary agent0.5～2h, extract is for subsequent use, then each extract is mixed, and the dregs of a decoction discard.
5. preparation method according to claim 2, is characterized in that, described water extraction comprises water extraction, for the first time water extraction three times:Add after a small amount of water soaking 0～1.5h to traditional Chinese medicine ingredients B, continue to add adding water to 6～15 times of recipe quantities and extract, when extractionBetween 1～2h, extract is for subsequent use; Water extraction for the second time: add the water of 5～12 times of recipe quantities with the dregs of a decoction that water extraction produces for the first time,Carry out second extraction, extraction time 0.5～1.5h, extract is for subsequent use; Water extraction for the third time: add with the dregs of a decoction that water extraction produces for the second timeEnter the water of 5～12 times of recipe quantities, carry out three times and extract, extraction time 0.5～1.5h, extract is for subsequent use, then each time is extractedLiquid mixes, and the dregs of a decoction discard.
6. according to the preparation method described in any one in claim 2～5, it is characterized in that, in described step (2) concentrated,Dry referring to carried out reduced pressure concentration by described alcohol extract in 50～70 DEG C, is concentrated to relative density to 1.3～1.35, is then placed inIn 55～65 DEG C of vacuum, carry out vacuum drying.
7. according to the preparation method described in any one in claim 2～5, it is characterized in that, in described step (3) concentrated,Dry referring to carried out reduced pressure concentration by described water extraction liquid in 60～80 DEG C, is concentrated to relative density to 1.3～1.35, is then placed inIn 65～75 DEG C of vacuum, carry out vacuum drying.
8. according to the preparation method described in any one in claim 2～5, it is characterized in that the mixing system that step (4) is describedGrain adopts wet granulation, after interpolation supplementary product starch, adds ethanol to granulate again, and the particle of making is crossed 60～100 mesh sieves, is then placed in, higher than carrying out the dry of particle under the environment of 50 DEG C, the volatile oil a obtaining with step (1) does not mix.
A compound blood pressure reducing as claimed in claim 1 fall capsule for the preparation of in treatment-refractory hypertension drug shouldWith.
10. the compound blood pressure reducing capsule being obtained by the preparation method described in any one in claim 2～8 is for the preparation of auxiliaryHelp the application in treatment refractory hypertension medicine.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657079A (en) * | 2004-02-18 | 2005-08-24 | 袁泽依 | Method for treating hypertension disease |
| CN101401910A (en) * | 2008-09-23 | 2009-04-08 | 罗先德 | Medicament for treating hypertension |
| CN101468054A (en) * | 2007-12-28 | 2009-07-01 | 上海雷允上科技发展有限公司 | Wild chrysanthemum effective component composition, preparation method and use thereof as well as medicinal preparation containing the same |
| CN103041136A (en) * | 2013-02-02 | 2013-04-17 | 黄晶晶 | Chinese medicine preparation for treating hypertension |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1657079A (en) * | 2004-02-18 | 2005-08-24 | 袁泽依 | Method for treating hypertension disease |
| CN101468054A (en) * | 2007-12-28 | 2009-07-01 | 上海雷允上科技发展有限公司 | Wild chrysanthemum effective component composition, preparation method and use thereof as well as medicinal preparation containing the same |
| CN101401910A (en) * | 2008-09-23 | 2009-04-08 | 罗先德 | Medicament for treating hypertension |
| CN103041136A (en) * | 2013-02-02 | 2013-04-17 | 黄晶晶 | Chinese medicine preparation for treating hypertension |
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