CN103842521A - 选择适配体的组合物和方法 - Google Patents

选择适配体的组合物和方法 Download PDF

Info

Publication number
CN103842521A
CN103842521A CN201280038577.0A CN201280038577A CN103842521A CN 103842521 A CN103842521 A CN 103842521A CN 201280038577 A CN201280038577 A CN 201280038577A CN 103842521 A CN103842521 A CN 103842521A
Authority
CN
China
Prior art keywords
aptamer
nucleic acid
composition
target
stranded nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201280038577.0A
Other languages
English (en)
Chinese (zh)
Inventor
Y-H.张
L.田
R.王
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mediomics LLC
Original Assignee
Mediomics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mediomics LLC filed Critical Mediomics LLC
Publication of CN103842521A publication Critical patent/CN103842521A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201280038577.0A 2011-07-22 2012-07-23 选择适配体的组合物和方法 Pending CN103842521A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161510796P 2011-07-22 2011-07-22
US61/510796 2011-07-22
PCT/US2012/047840 WO2013016280A2 (en) 2011-07-22 2012-07-23 Compositions and methods for selecting aptamers

Publications (1)

Publication Number Publication Date
CN103842521A true CN103842521A (zh) 2014-06-04

Family

ID=47601735

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280038577.0A Pending CN103842521A (zh) 2011-07-22 2012-07-23 选择适配体的组合物和方法

Country Status (5)

Country Link
US (1) US20140243208A1 (https=)
EP (1) EP2734646A4 (https=)
CN (1) CN103842521A (https=)
IN (1) IN2014CN00312A (https=)
WO (1) WO2013016280A2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
EP1907410B1 (en) 2005-06-10 2012-07-04 Saint Louis University Methods for the selection of aptamers
US8993245B2 (en) 2008-11-21 2015-03-31 Mediomics, Llc Biosensor for detecting multiple epitopes on a target
WO2011100561A1 (en) 2010-02-12 2011-08-18 Saint Louis University Molecular biosensors capable of signal amplification
WO2016040830A1 (en) 2014-09-12 2016-03-17 Mediomics, Llc Molecular biosensors with a modular design
GB201705764D0 (en) 2017-04-10 2017-05-24 Imp Innovations Ltd Analyte detection method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050009050A1 (en) * 2003-04-18 2005-01-13 James Nadeau Immuno-amplification
US20050089890A1 (en) * 1998-05-20 2005-04-28 Cubicciotti Roger S. Multimolecular devices and drug delivery systems
US20100021899A1 (en) * 2006-09-27 2010-01-28 National University Corporation Tokyo University Of Agriculture And Technology Method of assaying target substance in sample, aptamer molecule and method of constructing the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2046998B1 (en) * 2006-08-09 2013-10-02 Saint Louis University Molecular biosensors for detecting macromolecules and other analytes
WO2011100561A1 (en) * 2010-02-12 2011-08-18 Saint Louis University Molecular biosensors capable of signal amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050089890A1 (en) * 1998-05-20 2005-04-28 Cubicciotti Roger S. Multimolecular devices and drug delivery systems
US20050009050A1 (en) * 2003-04-18 2005-01-13 James Nadeau Immuno-amplification
US20100021899A1 (en) * 2006-09-27 2010-01-28 National University Corporation Tokyo University Of Agriculture And Technology Method of assaying target substance in sample, aptamer molecule and method of constructing the same

Also Published As

Publication number Publication date
WO2013016280A3 (en) 2013-03-21
US20140243208A1 (en) 2014-08-28
EP2734646A2 (en) 2014-05-28
EP2734646A4 (en) 2014-12-24
WO2013016280A2 (en) 2013-01-31
IN2014CN00312A (https=) 2015-04-03

Similar Documents

Publication Publication Date Title
US8105982B2 (en) Aptamers and methods for their in vitro selection and uses thereof
ES2848531T3 (es) Métodos mejorados para la selección de proteínas de unión
CN103842521A (zh) 选择适配体的组合物和方法
US8404448B2 (en) DNA aptamer specifically binding to human cardiac troponin I
JP7628153B2 (ja) ｍＲＮＡディスプレイ抗体ライブラリー及び方法
CA2737035A1 (en) Improved method of rna display
CN102333790A (zh) 检测维生素d的分析方法及用于其的抗体
CN107430130A (zh) 方法
CN114127282A (zh) 适体的筛选方法和使用适体的免疫分析方法
CN105039346B (zh) 一种与三聚氰胺特异结合的核酸适配体及应用
KR102133618B1 (ko) 히스티딘 태그에 결합하는 dna 앱타머와 그것의 용도
KR20180054018A (ko) 글리포세이트 특이 결합 앱타머 및 이의 용도
JP6793917B2 (ja) アプタマー及び抗体検出方法
CA3248461A1 (en) A PRODUCTIBLE APTAMER SELECTION METHOD USING CLOSED SEQUENCE SOLUTION SPACES
US20180245070A1 (en) Dna display and methods thereof
KR101069589B1 (ko) Ｃｅａ에 특이적으로 결합하는 단일 가닥 ｄｎａ 압타머
US11041849B2 (en) Methods and systems for identifying candidate nucleic acid agent
KR101069590B1 (ko) Ｃｅａ에 특이적으로 결합하는 단일 가닥 ｄｎａ 압타머
CN106520777A (zh) 一种单链抗体及其在特异性检测正平行型g‑四链体中的应用
US20030091986A1 (en) Identification of expressed genes using phage display
WO2018038076A1 (ja) Ｅ－カドヘリン結合型核酸アプタマーを用いる細胞足場材料
KR101069591B1 (ko) Ｃｅａ에 특이적으로 결합하는 단일 가닥 ｄｎａ 압타머

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20140604