CN103842493A - Living cell cryopreservation tool - Google Patents
Living cell cryopreservation tool Download PDFInfo
- Publication number
- CN103842493A CN103842493A CN201280048620.1A CN201280048620A CN103842493A CN 103842493 A CN103842493 A CN 103842493A CN 201280048620 A CN201280048620 A CN 201280048620A CN 103842493 A CN103842493 A CN 103842493A
- Authority
- CN
- China
- Prior art keywords
- viable cell
- mentioned
- freezing preservation
- maintaining part
- recess
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/22—Means for packing or storing viable microorganisms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
- A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mechanical Engineering (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A living cell cryopreservation tool (1) is provided with a main body (3) formed from a cold-resistant material, and a living cell holding part (2) formed from a cold-resistant material. The living cell holding part (2) is provided with an elongated living cell attachment holding part (22). The living cell attachment holding part (22) has: a plurality of concave parts (28a, 28b, 28c, 28d, 28e) for storing living cells and disposed along the lengthwise direction of the living cell attachment holding part (22); and excess cryopreservation liquid discharge paths (29a, 29b, 29c, 29d, 29e, 29f) connected to the concave parts (28a, 28b, 28c, 28d, 28e) for storing living cells.
Description
Technical field
The present invention relates to a kind of by ovum such as mammiferous ovum, embryos, the stem cells such as hemopoietic stem cell, multipotential stem cell, sperm etc., the cell freezing that various viable cell are freezing to be used while preserving is preserved apparatus.
Background technology
The freezing preservation of mammal embryo can be preserved the genetic resources of specific system, kind.In addition be also effective for the animal species that maintains endangered crisis.And, in the mankind's infertile treatment, be also useful.
As the freezing and storing method of mammal embryo, at patent documentation 1(TOHKEMY 2000-189155) in such method proposed: utilize the minimum vetrifying solution of enough parcel mammal embryos or ovum these embryos or ovum to be bonded to the internal surface of the freezing preservation use such as freezing suction pipe, freezing bottle or the freeze pipe container after sterilising treatment, by this freezing preservation container sealing, then make this container contact with liquid nitrogen and this container be carried out cooling rapidly.And, melting in method, the freezing preservation container that utilizes aforesaid method to preserve is taken out from above-mentioned liquid nitrogen, make the open-ended of container, to the direct diluent of 33 DEG C～39 DEG C of injections in this container, the freeze-thaw of embryo or ovum is diluted.Adopt the method, have can without because of virus, the sorrow that infects of bacterium preserve and melt dilution mammal embryo or the so excellent effect of ovum with higher survival rate.
But utilizing the minimum vetrifying solution of the ovum such as enough parcel mammal embryo or ovums to be bonded at the operation of the internal surface of the freezing preservation use such as freezing suction pipe, freezing bottle or freeze pipe container may not be easy.
Formerly technical literature
Patent documentation
Patent documentation 1: TOHKEMY 2000-189155
Patent documentation 2: TOHKEMY 2002-315573(WO02-085110A1)
Patent documentation 3: the open 2004-0259072 of the TOHKEMY 2004-329202(U.S.)
Summary of the invention
the problem that invention will solve
Therefore, present inventor has proposed patent documentation 2(TOHKEMY 2002-315573, WO02-085110A1).The egg freezing of patent documentation 2 is preserved apparatus 1 and is adhered to and kept forming with band 3 and cylindrical member 4 by main part 2, ovum, this main part 2 is formed by winter hardiness material, this ovum adheres to the one end that keeps being arranged on band 3 main part 2, formed by flexible, transparent and resistance to liquid nitrogen material, this cylindrical member 4 is formed by winter hardiness material, and it can wrap up ovum and adhere to and keep with band 3 and install and remove this one end that is arranged on freely main part 2 being blocked.
In addition, present inventor has also proposed patent documentation 3(TOHKEMY 2004-329202, the open 2004-0259072 of the U.S.).The egg freezing of patent documentation 3 is preserved apparatus 1 and is had the egg freezing preservation being formed by the material of resistance to liquid nitrogen pipe 2 and the metal tubular protection processed member 3 for the protection of pipe 2.Pipe 2 has the ovum that main part 21 and internal diameter are 0.1mm～0.5mm to be preserved with small diameter part 22, and can be sealed by the tip side at small diameter part, can be sealed at main part 21.Tubular protection member 3 has cylindrical portion 31 and half cylindrical portion 32, and this cylindrical portion 31 is for the top ends of the small diameter part 22 of containing tube 2, and this half cylindrical portion 32 is for receiving the part of not received by cylindrical portion 31 of small diameter part 22 and the top ends 21a of main part 21.
It is effective that apparatus is preserved in the egg freezing of patent documentation 2 and patent documentation 3.
But egg freezing operation can be more easily carried out in expectation, and can prevent reliably that the egg freezing that the ovum that is frozen departs from from preserving apparatus.
The object of the invention is to, provide a kind of viable cell is loaded in the processing ease of the freezing preservation apparatus of viable cell, in the time of the operation of freezing viable cell, viable cell can not depart from, and can promptly make the freezing preservation apparatus of the freezing viable cell of viable cell.
for the scheme of dealing with problems
The freezing preservation apparatus of viable cell achieving the above object is as follows.
The freezing preservation apparatus of a kind of viable cell, it has the main part being formed by winter hardiness material and the viable cell maintaining part being formed by winter hardiness material, wherein, above-mentioned viable cell maintaining part has elongated viable cell and adheres to maintaining part, above-mentioned viable cell adheres to maintaining part and has viable cell storage recess and the freezing preservation liquid drain passageway of residue, the storage of this viable cell is adhered at above-mentioned viable cell with recess and on the length direction of maintaining part, is provided with multiplely, and the freezing preservation liquid of this residue drain passageway and above-mentioned viable cell are received and be communicated with recess.
Brief description of the drawings
Fig. 1 is the front view of the freezing preservation apparatus of the viable cell of embodiments of the invention.
Fig. 2 is the enlarged front view of the tip side part of the freezing preservation member of the viable cell shown in Fig. 1.
Fig. 3 is the left view of the freezing preservation member of the viable cell shown in Fig. 2.
Fig. 4 is the A-A sectional view of Fig. 2.
Fig. 5 is the B-B sectional view of Fig. 2.
Fig. 6 is the amplification plan view of the freezing preservation member of the viable cell shown in Fig. 2.
Fig. 7 is the C-C amplification view of Fig. 2.
Fig. 8 is the enlarged front view of the top ends of the freezing preservation member of the viable cell shown in Fig. 1.
Fig. 9 is the D-D sectional view of Fig. 8.
To be the freezing preservation member of the viable cell of another embodiment of the present invention be truncated with recess place and the amplification sectional elevation that obtains in viable cell storage Figure 10.
Figure 11 is the amplification longitudinal section of the freezing preservation member of the viable cell of another embodiment of the present invention.
Figure 12 is the enlarged front view of the top ends of the freezing preservation apparatus of the viable cell of another embodiment of the present invention.
Figure 13 is the E-E sectional view of Figure 12.
Figure 14 is the F-F sectional view of Figure 12.
Embodiment
Use illustrated embodiment that the freezing preservation apparatus of viable cell of the present invention is described.
The viable cell maintaining part (ovum maintaining part) 2 that the freezing preservation apparatus 1 of viable cell of the present invention has the main part 3 being formed by winter hardiness material and formed by winter hardiness material.Ovum maintaining part 2 has elongated ovum and adheres to maintaining part 22, and ovum adheres to maintaining part 22 to be had and on ovum adheres to the length direction of maintaining part 22, be provided with multiple viable cell storage recess (ovum storage recess) 28a, 28b, 28c, 28d, 28e and receive with ovum the freezing preservation liquid of the residue drain passageway 29a, 29b, 29c, 29d, 29e, the 29f that are communicated with recess 28a, 28b, 28c, 28d, 28e.
Particularly, it is that apparatus is preserved in egg freezing that the cell freezing of this embodiment is preserved apparatus 1, and viable cell retaining member 2 is ovum maintaining part.In addition, cell freezing of the present invention is preserved apparatus and be can be used in the ovum such as freezing preservation ovum, embryo, the stem cells such as hemopoietic stem cell, multipotential stem cell, and sperm etc., various cells, particularly can be used in freezing preservation viable cell as described above.
As shown in Fig. 1～Fig. 5, the freezing preservation apparatus 1 of viable cell has the main part 3 being formed by winter hardiness material and the viable cell maintaining part 2 being formed by winter hardiness material.In this embodiment, as shown in Fig. 2～Fig. 5 (particularly Fig. 4), be formed with the 21a of hole portion that extends specified length to top orientation at the base end part of viable cell maintaining part 2, be provided with the protuberance 24 that can enter into the extension specified length in the above-mentioned hole 21a of portion in the top ends of main part 3.And, by the protuberance of main part 3 24 being inserted in the 21a of hole portion of viable cell maintaining part 2, both are fixed together.
The cross section of viable cell maintaining part 2 is essentially rectangular shape, its have the base end part that is connected with main part 3 as described above and certainly this base end part adhere to maintaining part 22 to the outstanding viable cell of tip side.And in this embodiment, viable cell adheres to maintaining part 22 for band shape (lamellar) elongated and that width is narrower.And its surface forms viable cell and adheres to maintenance face.
And in the freezing preservation apparatus 1 of viable cell of the present invention, as shown in Fig. 2～Fig. 9, the surface of adhering to maintaining part 22 at viable cell is provided with and is provided with in the longitudinal direction multiple viable cell storage recess 28a, 28b, 28c, 28d, 28e.As shown in Fig. 4 and Fig. 9, recess 28a for viable cell storage, 28b, 28c, 28d, 28e are hemispheric recess roughly.In addition, multiple recess 28a, 28b, 28c, 28d, 28e preserve the top of apparatus 1 and arrange with linearity to base end side by the position of base end side specified length from specific activity cell freezing, and each recess is roughly uniformly-spaced to separate.In this embodiment, recess is provided with multiple, but also can be 1.In addition,, in the situation that being provided with multiple recess, be preferably 2～8.The degree of depth of recess is preferably 0.05mm～0.5mm, and the opening diameter of the upper surface of recess is preferably 0.1mm～0.5mm.In addition, the spacing distance between recess is preferably 1mm～3mm.
And, in this embodiment, as shown in Fig. 7 and Fig. 9, the rear side part that is formed with the part of viable cell storage recess 28a, 28b, 28c, 28d, 28e is formed as bellying 38a, 38b, 38c, 38d, 38e, forming the viable cell storage part of recess is not thin-walled, and has sufficient intensity.In addition, viable cell storage recess also can be set, and be formed flatly the surface of the freezing preservation apparatus 1 of viable cell, its tabular surface is adhered to maintaining part as viable cell.In addition, the width that viable cell adheres to maintaining part 22 is preferably 0.4mm～1.0mm, and length is preferably 5mm～30mm, and the thickness of entirety and the thickness of recess part are preferably 0.08mm～1.0mm.In addition, the length of the base end part of the heavy wall of viable cell maintaining part 2 is preferably 5mm～30mm, and the length of main part is preferably 20mm～100mm.
And, in the freezing preservation apparatus 1 of viable cell of this embodiment, as shown in Figure 6 to 8, have be located at viable cell adhere to maintaining part 22 the part that is formed with viable cell storage recess 28a, 28b, 28c, 28d, 28e both sides and adhere to two sidepiece bellyings 35,36 of the length direction extension of maintaining part 22 along viable cell.Therefore, the freezing preservation apparatus 1 of viable cell is owing to having bellying in viable cell storage with the both sides of recess, therefore can be suppressed at reliably mounting when viable cell viable cell move to side direction, and can also prevent that the viable cell after mounting from deviating from from viable cell housing recess.
And, in the freezing preservation apparatus 1 of viable cell of this embodiment, as shown in Fig. 1～Fig. 9 (particularly Fig. 6 and Fig. 7), viable cell adheres to maintaining part 22 and has the ratio viable cell storage recess 28a that is located at viable cell and adheres to maintaining part 22,28b, 28c, 28d, the 28e protuberance 27 by the position of tip side.Protuberance 27 adheres to the top of maintaining part 22 to be given prominence to upper surface side (forming that side of viable cell storage recess) from viable cell.In addition, as shown in Figure 9, the scarp that the top end face of protuberance 27 is formed as tilting slightly to base end side.By forming such protuberance 27, while movement to tip side if viable cell departs from from viable cell incorporating section, can prevent that it from falling from the freezing preservation apparatus 1 of viable cell, in other words can prevent that it from adhering to maintaining part 22 from viable cell and falling.Particularly, in the freezing preservation apparatus 1 of viable cell of this embodiment, by be located at as described above viable cell and adhere to bellying 35,36 teamworks of the both sides of maintaining part 22, thereby prevent that viable cell from falling.
And, in the freezing preservation apparatus 1 of viable cell of this embodiment, as shown in Fig. 2～Fig. 9 (particularly Fig. 8 and Fig. 9), viable cell adheres to maintaining part 22 to be had with viable cell and receives the freezing preservation liquid of the residue drain passageway being communicated with recess 28a, 28b, 28c, 28d, 28e.In the freezing preservation apparatus 1 of viable cell of this embodiment, remain freezing preservation liquid drain passageway and formed by slot part 29a, 29b, 29c, 29d, 29e, the 29f of the length direction extension of adhering to maintaining part 22 along viable cell.And in this embodiment, slot part 29b, 29c, 29d, 29e link up adjacent viable cell storage with recess.Particularly, slot part 29b is communicated with recess 28a and recess 28b, and slot part 29c is communicated with recess 28b and recess 28c, and slot part 29d is communicated with recess 28c and recess 28d, and slot part 29e is communicated with recess 28d and recess 28e.
And, in this embodiment, as shown in Fig. 8 and Fig. 9, viable cell adheres to maintaining part 22 and has the slot part 29a extending to tip side by the viable cell storage recess 28a of tip side that adheres to maintaining part 22 from viable cell, and the end of this slot part 29a arrives above-mentioned protuberance or extends near above-mentioned protuberance.And in this embodiment, viable cell adheres to maintaining part 22 and has the slot part 29f extending to base end side by the viable cell storage recess 28e of base end side that adheres to maintaining part 22 from viable cell.
Such receive with viable cell the freezing preservation liquid of the residue drain passageway being communicated with recess by arranging, together being incorporated in the viable cell storage remainder of the freezing preservation liquid in recess with viable cell flow in this slot part, therefore, can suppress viable cell and be covered by excessive freezing preservation liquid, thereby can promptly carry out freezing.And because adjacent viable cell storage is communicated with by slot part with recess, therefore, freezing preservation liquid becomes easily and moves from viable cell storage recess, and, remain in multiple viable cell storage freezing preservation liquid measures of recess and also become even.The width of slot part is preferably 100 μ m～500 μ m, and the degree of depth of slot part is preferably 50 μ m～500 μ m.
In the freezing preservation apparatus 1 of viable cell of above-described embodiment, as shown in Fig. 6, Fig. 7 and Fig. 9 (particularly Fig. 7), slot part 29a, 29b, 29c, 29d, 29e do not arrive the bottom of viable cell storage recess 28a, 28b, 28c, 28d, 28e.So, can stockpile some freezing preservation liquid with the bottom of recess 28a, 28b, 28c, 28d, 28e in viable cell storage.
But, be not limited to mode as described above, the freezing preservation apparatus 10 of viable cell that also can be shown in Figure 10 and Figure 11, slot part 29a, 29b, 29c, 29d, 29e arrive the bottom of viable cell storage recess 28a, 28b, 28c, 28d, 28e.So, can further reduce the freezing preservation liquid stockpiling in viable cell storage recess 28a, 28b, 28c, 28d, 28e.
In addition the mode that, viable cell storage receives with recess and with this viable cell the freezing preservation liquid of the residue drain passageway being communicated with recess is not limited to mode as described above.
It can be also for example the such type of the freezing preservation apparatus 20 of the viable cell shown in Figure 12～Figure 14.Figure 12 is the enlarged front view of the top ends of the freezing preservation apparatus of the viable cell of another embodiment of the present invention.Figure 13 is the E-E sectional view of Figure 12.Figure 14 is the F-F sectional view of Figure 12.
In the freezing preservation apparatus 20 of viable cell, viable cell adheres to maintaining part 22 and has the multiple short cylinder shape protuberances that are formed on its upper surface, is formed with viable cell storage recess 51,52,53,54,55 in the inside of these protuberances.And, there are two slit 61a, 61b being communicated with recess 51 at the sidewall of protuberance, two slit 62a, 62b being communicated with recess 52, two slit 63a, 63b being communicated with recess 53, two slit 64a, 64b being communicated with recess 54, and two slit 65a, 65b being communicated with recess 55.In addition, each slit is communicated in the bottom of place recess separately.In addition, the slit being communicated with recess can be also 1, but preferably arranges more than two.In addition, the degree of depth of recess is preferably 0.05mm～0.5mm.And, in the freezing preservation apparatus 20 of this viable cell, be formed with the recess 56 extending in the mode of extending along axis direction and pass through from the below of the bottom surface of recess 51 in its rear side (not being formed with the side side of short cylinder shape protuberance).Therefore, the part that forms the bottom surface of recess 51 is thinner wall section, makes to the heat conduction of recess good.
The using method of the freezing preservation apparatus 1 of viable cell of the present invention then, is described.
In this explanation, describe as an example of the situation of freezing preservation viable cell (specifically ovum) example.
First, extract multiple ovums as viable cell, carry out the intracellular fluid of ovum to be replaced into the operation of balance liquid, then carry out extracellular fluid to be replaced into the operation of vetrifying solution.Then, under the microscope, ovum and a small amount of vetrifying solution are together configured be attached to the viable cell of being located at the freezing preservation apparatus 1 of viable cell to adhere to each viable cell storage recess 28a, 28b, 28c, 28d, the 28e of maintaining part 22.Freezing the viable cell that is attached with ovum preservation apparatus 1 is immersed in preprepared liquid nitrogen its freezing (vitrifying) from tip side (viable cell maintaining part 22 sides).By contacting with liquid nitrogen, make viable cell sharply cooling.After in the cylindrical container that freezing viable cell that is attached with freezing viable cell preservation apparatus 1 is accommodated in to other preparation, then be accommodated in accommodating container (cane), accommodating container put into liquid nitrogen container and save.
utilizability in industry
The freezing preservation apparatus of viable cell of the present invention is as follows.
(1) the freezing preservation apparatus of a kind of viable cell, it has the main part being formed by winter hardiness material and the viable cell maintaining part being formed by winter hardiness material, in the freezing preservation apparatus of this viable cell, above-mentioned viable cell maintaining part has elongated viable cell and adheres to maintaining part, above-mentioned viable cell adheres to maintaining part and has viable cell storage recess and the freezing preservation liquid drain passageway of residue, the storage of this viable cell is adhered at above-mentioned viable cell with recess and on the length direction of maintaining part, is provided with multiplely, and the freezing preservation liquid of this residue drain passageway and above-mentioned viable cell are received and be communicated with recess.
In the freezing conservator of above-mentioned viable cell, viable cell adheres to maintaining part and has multiple viable cell storage recesses, thus, in viable cell configuration operation process, viable cell adheres to maintaining part becomes the target of configuration viable cell, thereby can be configured well operation, and, because the movement of the viable cell that is incorporated in viable cell storage recess is restricted, therefore, can prevent viable cell after freezing operation process in move, depart from.And, receive owing to having with viable cell the freezing preservation liquid of the residue drain passageway being communicated with recess, therefore, can in recess, not stockpile excessive freezing preservation liquid, can be promptly that viable cell is cooling in the time of freezing operation.
And embodiments of the present invention also can be as follows.
(2), according to the freezing preservation apparatus of viable cell above-mentioned (1) Suo Shu, wherein, the freezing preservation liquid of above-mentioned residue drain passageway is to be communicated with and to adhere to along above-mentioned viable cell the slot part of the length direction extension of maintaining part with above-mentioned viable cell storage recess.
(3) according to the freezing preservation apparatus of viable cell above-mentioned (1) or (2) Suo Shu, wherein, above-mentioned viable cell maintaining part has two sidepiece bellyings, these two sidepiece bellyings are located at the both sides that above-mentioned viable cell adheres to the part of the above-mentioned viable cell storage of being formed with of maintaining part recess, and adhere to the length direction extension of maintaining part along above-mentioned viable cell.
(4), according to the freezing preservation apparatus of viable cell described in any one in above-mentioned (1)～(3), wherein, above-mentioned viable cell adheres to maintaining part to be had and is located at than the protuberance by the position of tip side by the above-mentioned viable cell storage recess of tip side.
(5), according to the freezing preservation apparatus of viable cell described in any one in above-mentioned (2)～(4), wherein, above-mentioned slot part couples together adjacent above-mentioned viable cell storage with recess.
(6), according to the freezing preservation apparatus of viable cell described in any one in above-mentioned (2)～(5), wherein, above-mentioned viable cell adheres to maintaining part to be had from the slot part extending to tip side by the above-mentioned viable cell storage recess of tip side.
(7), according to the freezing preservation apparatus of viable cell described in any one in above-mentioned (2)～(6), wherein, above-mentioned viable cell adheres to maintaining part to be had from the slot part extending to base end side by the above-mentioned viable cell storage recess of base end side.
(8) according to the freezing preservation apparatus of viable cell described in any one in above-mentioned (1)～(7), wherein, above-mentioned winter hardiness material is resistance to liquid nitrogen material.
(9) according to the freezing preservation apparatus of viable cell described in any one in above-mentioned (1)～(8), wherein, it is lamellar that above-mentioned viable cell adheres to maintaining part.
Claims (9)
1. the freezing preservation apparatus of viable cell, it has the main part being formed by winter hardiness material and the viable cell maintaining part being formed by winter hardiness material, and the freezing preservation apparatus of this viable cell is characterised in that,
Above-mentioned viable cell maintaining part has elongated viable cell and adheres to maintaining part, above-mentioned viable cell adheres to maintaining part and has viable cell storage recess and the freezing preservation liquid drain passageway of residue, the storage of this viable cell is adhered at above-mentioned viable cell with recess and on the length direction of maintaining part, is provided with multiplely, and the freezing preservation liquid of this residue drain passageway and above-mentioned viable cell are received and be communicated with recess.
2. the freezing preservation apparatus of viable cell according to claim 1, wherein,
The freezing preservation liquid of above-mentioned residue drain passageway is to be communicated with and to adhere to along above-mentioned viable cell the slot part of the length direction extension of maintaining part with above-mentioned viable cell storage recess.
3. the freezing preservation apparatus of viable cell according to claim 1 and 2, wherein,
Above-mentioned viable cell maintaining part has two sidepiece bellyings, and these two sidepiece bellyings are located at the both sides that above-mentioned viable cell adheres to the part of the above-mentioned viable cell storage of being formed with of maintaining part recess, and adheres to the length direction extension of maintaining part along above-mentioned viable cell.
4. according to the freezing preservation apparatus of the viable cell described in any one in claim 1～3, wherein,
Above-mentioned viable cell adheres to maintaining part to be had and is located at than the protuberance by the position of tip side by the above-mentioned viable cell storage recess of tip side.
5. according to the freezing preservation apparatus of the viable cell described in any one in claim 2～4, wherein,
Above-mentioned slot part couples together adjacent above-mentioned viable cell storage with recess.
6. according to the freezing preservation apparatus of the viable cell described in any one in claim 2～5, wherein,
Above-mentioned viable cell adheres to maintaining part to be had from the slot part extending to tip side by the above-mentioned viable cell storage recess of tip side.
7. according to the freezing preservation apparatus of the viable cell described in any one in claim 2～6, wherein,
Above-mentioned viable cell adheres to maintaining part to be had from the slot part extending to base end side by the above-mentioned viable cell storage recess of base end side.
8. according to the freezing preservation apparatus of the viable cell described in any one in claim 1～7, wherein,
Above-mentioned winter hardiness material is resistance to liquid nitrogen material.
9. according to the freezing preservation apparatus of the viable cell described in any one in claim 1～8, wherein,
It is lamellar that above-mentioned viable cell adheres to maintaining part.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2011219592 | 2011-10-03 | ||
| JP2011-219592 | 2011-10-03 | ||
| PCT/JP2012/075431 WO2013051520A1 (en) | 2011-10-03 | 2012-10-01 | Living cell cryopreservation tool |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103842493A true CN103842493A (en) | 2014-06-04 |
| CN103842493B CN103842493B (en) | 2015-09-23 |
Family
ID=48043678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280048620.1A Active CN103842493B (en) | 2011-10-03 | 2012-10-01 | Viable cell freezen protective apparatus |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9538746B2 (en) |
| EP (1) | EP2765181B1 (en) |
| JP (1) | JP5781621B2 (en) |
| KR (1) | KR101934031B1 (en) |
| CN (1) | CN103842493B (en) |
| HK (1) | HK1198177A1 (en) |
| WO (1) | WO2013051520A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114008185A (en) * | 2019-06-19 | 2022-02-01 | 株式会社先端生殖技术研究所 | Instrument for use in manipulating cells |
| WO2023036225A1 (en) * | 2021-09-10 | 2023-03-16 | 深圳拜尔洛克生物技术有限公司 | Device for cryopreservation or thawing and recovery of biological tissue |
| WO2023036223A1 (en) * | 2021-09-10 | 2023-03-16 | 深圳拜尔洛克生物技术有限公司 | Device for freezing preservation or unfreezing resuscitation of biological tissue |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3386297A1 (en) | 2015-12-07 | 2018-10-17 | CooperSurgical, Inc. | Low temperature specimen carriers and related methods |
| KR102288612B1 (en) | 2017-06-12 | 2021-08-11 | 미쓰비시 세이시 가부시키가이샤 | Jig for cryopreservation of cells or tissues |
| US11116206B2 (en) | 2018-10-01 | 2021-09-14 | Cook Medical Technologies Llc | Cryocontainer |
| USD918707S1 (en) * | 2018-12-04 | 2021-05-11 | Jun Tao | Vitrification and storage device |
| JP2022515526A (en) | 2018-12-28 | 2022-02-18 | オーバーチュア ライフ，インコーポレイテッド | Cryopreservation device for oocytes and embryos during cryopreservation |
| USD951481S1 (en) | 2020-09-01 | 2022-05-10 | TMRW Life Sciences, Inc. | Cryogenic vial |
| USD963194S1 (en) | 2020-12-09 | 2022-09-06 | TMRW Life Sciences, Inc. | Cryogenic vial carrier |
| AU2021396319B2 (en) | 2020-12-10 | 2024-07-18 | TMRW Life Sciences, Inc. | Specimen holder with wireless transponder for attachment to secimen collection body |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101087658A (en) * | 2004-12-21 | 2007-12-12 | 麦吉尔大学 | Retractable tool and method for manipulating developmental cells in a process of cryopreservation |
| US20090298116A1 (en) * | 2008-05-30 | 2009-12-03 | Ye Fang | Cell culture apparatus having different micro-well topography |
| WO2010047133A1 (en) * | 2008-10-24 | 2010-04-29 | 株式会社クラレ | Cell storage method and cell transport method |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000189155A (en) | 1998-12-25 | 2000-07-11 | Livestock Improvement Association Of Japan Inc | Preservation of mammalian embryo or ovum and thawing dilution of frozen mammalian embryo or ovum |
| JP4373025B2 (en) | 2001-04-18 | 2009-11-25 | 株式会社北里サプライ | Egg cryopreservation tool and cylindrical member holding device |
| JP4355186B2 (en) | 2003-04-15 | 2009-10-28 | 株式会社北里サプライ | Egg cryopreservation tool |
| US20090123992A1 (en) | 2007-11-12 | 2009-05-14 | Milton Chin | Shape-Shifting Vitrification Device |
| EP3510865A3 (en) * | 2008-02-25 | 2020-01-15 | Genea IP Holdings Pty Limited | Cryopreservation of biological cells and tissues |
| US8030063B2 (en) * | 2008-06-18 | 2011-10-04 | The Cleveland Clinic Foundation | Systems and methods for vitrifying tissue |
| JP5062164B2 (en) * | 2008-12-25 | 2012-10-31 | ニプロ株式会社 | Cell device and method for manufacturing cell device |
| US20110129811A1 (en) * | 2009-12-01 | 2011-06-02 | Scitech Invention, Llc | Cryopreservation and Storage Systems and Methods |
| US20110196358A1 (en) | 2010-02-09 | 2011-08-11 | Enrique Criado Scholz | Closed ultra-rapid cell vitrification device and sealing procedure of the device |
| WO2011140299A1 (en) * | 2010-05-06 | 2011-11-10 | Butler Brad K | Carrier comprising a seal for cyrogenic storage |
| JP5935313B2 (en) * | 2010-12-17 | 2016-06-15 | ニプロ株式会社 | Cryopreservation equipment |
-
2012
- 2012-10-01 CN CN201280048620.1A patent/CN103842493B/en active Active
- 2012-10-01 US US14/349,169 patent/US9538746B2/en active Active
- 2012-10-01 KR KR1020147008506A patent/KR101934031B1/en active IP Right Grant
- 2012-10-01 JP JP2013537503A patent/JP5781621B2/en active Active
- 2012-10-01 EP EP12838223.1A patent/EP2765181B1/en active Active
- 2012-10-01 WO PCT/JP2012/075431 patent/WO2013051520A1/en active Application Filing
-
2014
- 2014-11-19 HK HK14111680.6A patent/HK1198177A1/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101087658A (en) * | 2004-12-21 | 2007-12-12 | 麦吉尔大学 | Retractable tool and method for manipulating developmental cells in a process of cryopreservation |
| US20090298116A1 (en) * | 2008-05-30 | 2009-12-03 | Ye Fang | Cell culture apparatus having different micro-well topography |
| WO2010047133A1 (en) * | 2008-10-24 | 2010-04-29 | 株式会社クラレ | Cell storage method and cell transport method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114008185A (en) * | 2019-06-19 | 2022-02-01 | 株式会社先端生殖技术研究所 | Instrument for use in manipulating cells |
| WO2023036225A1 (en) * | 2021-09-10 | 2023-03-16 | 深圳拜尔洛克生物技术有限公司 | Device for cryopreservation or thawing and recovery of biological tissue |
| WO2023036223A1 (en) * | 2021-09-10 | 2023-03-16 | 深圳拜尔洛克生物技术有限公司 | Device for freezing preservation or unfreezing resuscitation of biological tissue |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101934031B1 (en) | 2019-01-07 |
| US9538746B2 (en) | 2017-01-10 |
| WO2013051520A1 (en) | 2013-04-11 |
| CN103842493B (en) | 2015-09-23 |
| JPWO2013051520A1 (en) | 2015-03-30 |
| HK1198177A1 (en) | 2015-03-13 |
| EP2765181A1 (en) | 2014-08-13 |
| EP2765181B1 (en) | 2016-07-13 |
| JP5781621B2 (en) | 2015-09-24 |
| US20140212962A1 (en) | 2014-07-31 |
| KR20140070575A (en) | 2014-06-10 |
| EP2765181A4 (en) | 2015-06-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103842493B (en) | Viable cell freezen protective apparatus | |
| CN103874761B (en) | Cell freezing preserves apparatus | |
| CN103857783B (en) | Viable cell freezen protective apparatus | |
| EP1619243B1 (en) | Implement and method for freezing and storing egg | |
| JP4324181B2 (en) | Egg cryopreservation tool | |
| EP3196290B1 (en) | Tool for cryopreserving collected biological tissue, and method for cryopreserving tissue fragment | |
| US20060019233A1 (en) | Delivery of high cell mass in a syringe and related methods of cryopreserving cells | |
| JP7108318B2 (en) | Biological cell cryopreservation device and biological cell cryopreservation device | |
| EP3772934B1 (en) | Carrier for freezing, storing, transporting, and thawing biological products storage bags | |
| JP5224703B2 (en) | Collected tissue piece vitrification cryopreservation tool, tissue piece cryopreservation plate, and harvested tissue piece vitrification cryopreservation method | |
| JP3173617U (en) | Cell freezing tool | |
| CN212232860U (en) | Freezing carrying rod for ultralow-temperature freezing and closed preservation of biological samples | |
| JP4498260B2 (en) | Method for sealing vitrification tools |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1198177 Country of ref document: HK |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: GR Ref document number: 1198177 Country of ref document: HK |