CN103837610A - Method for determining shikimic acid of pulse-activating injection - Google Patents
Method for determining shikimic acid of pulse-activating injection Download PDFInfo
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- CN103837610A CN103837610A CN201210486122.7A CN201210486122A CN103837610A CN 103837610 A CN103837610 A CN 103837610A CN 201210486122 A CN201210486122 A CN 201210486122A CN 103837610 A CN103837610 A CN 103837610A
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Abstract
The invention relates to a method for determining shikimic acid of a pulse-activating injection. The method comprises the steps: injecting the pulse-activating inspection in a high performance liquid chromatograph after properly diluting, and determining the content of the shikimic acid by adopting an external standard method.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to the assay method of shikimic acid in a kind of shengmai injection.
Background technology
Shengmai injection be develop according to the famous classical ancient prescription of the traditional Chinese medical science " Shengmai San " can injection for intravenous traditional Chinese medicine injection, by ginseng, the tuber of dwarf lilyturf, the fruit of Chinese magnoliavine three taste drug matchings and adopt modern science and technology to extract refining forming.
Ancient prescription " Shengmai San " is gone through more than one thousand years prolonged application, safe and effective, lasting.Ancient medicine is both for rescuing the severes such as thermal burn vigour, body fluid being damaged, feeble and impalpable pulse, again in order to the tonifying recipes as deficiency of both qi and yin patient.About the implication of Shengmai San side's name, says in Wu Kun " hospital examines ": " name is said raw arteries and veins person, bring about the desired sensation and fill with arteries and veins, mistake gas a little less than, therefore named it ".The arteries and veins person of the traditional Chinese medical science, main in the heart, and rise in lung, and so gas in lung, the survivor of relying, must provide nationality especially in kidney yin.Therefore " collection of prescriptions with notes " is said: " tonifying lung clears away heart-fire for lid the heart controlling blood vessels, the convergence of vessels in the lung, and gas fills and arteries and veins is multiple, therefore say raw arteries and veins also." can say, Shengmai San is named according to its effect.What raw arteries and veins also can be separated is multiple arteries and veins, and " the people exhausted person of arteries and veins that checkmates, take this can demutation it ".More figuratively understand effect of Shengmai San and the effect in emergency and severe disease rescues thereof.
Shikimic acid (Shikimic Acid, chemical name: 3,4,5-trihydroxy-1-cyclohexene-1-carboxylic acid) be in plant and microorganism, when phenylalanine, tyrosine and tryptophan biosynthesis, from phosphoenolpyruvate, the trihydroxy aromatic series intermediate product synthetic with erythrose-4-phosphate.Being the source of various aromatics, is also the important source material of some secondary metabolites.
At present, in shengmai injection, not yet report shikimic acid.Shikimic acid is mainly derived from Chinese anise, is the effective constituent of Chinese anise, is the primary raw material of antiviral drugs " Tamiflu ".The another fruit of Chinese magnoliavine and Chinese anise belong to Magnoliacea plant, measure in schisandra chinensis medicinal material and also contain a large amount of shikimic acid compositions, therefore think and shikimic acid validity feature composition in schisandra chinensis medicinal material can be controlled according to us.
In shengmai injection prescription, fruit of Chinese magnoliavine crude drug accounts for higher proportion, and very high according to surveying and determination in this product finished product.Therefore fruit of Chinese magnoliavine feature effective constituent, as testing index item, has more rationality than schizandrin assay in taking shikimic acid composition as shengmai injection quality standard.
Because Chinese medicine has complicated material base, therefore its quality control method cannot characterize its complicated biological substance composition, is in fact difficult to control its interior quality.Therefore,, how effectively the quality of specific control shengmai injection becomes the focus of people's research.
Summary of the invention
The object of the invention is to provide the assay method of shikimic acid in a kind of shengmai injection.The method is with strong points, for the quality standard control of traditional Chinese medicine provides new means.
Shikimic acid content assaying method in a kind of shengmai injection of the present invention, implement by the following technical programs:
A. get shengmai injection sample, suitably after dilution as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm 4micron, detect wavelength: 200 ~ 230nm; Column temperature: 25 ~ 40 DEG C; Flow velocity: 0.5 ~ 0.7ml/mim; The KH of mobile phase: 15 ~ 25mM/L
2pO
4solution (H
3pO
4adjust pH to 2.0 ~ 3.0);
D. adopt external standard method, utilize calculated by peak area to go out the content of shikimic acid.
Preferably, shikimic acid content assaying method in a kind of shengmai injection of the present invention, implement by the following technical programs:
A. get shengmai injection sample, dilute after 20-50 times, as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm, and 4micron, detects wavelength: 200-230nm; Column temperature: 25-40 DEG C; Flow velocity: 0.5-0.7ml/mim; The KH of mobile phase: 15 ~ 25mM/L
2pO
4solution, H
3pO
4adjust pH to 2.0 ~ 3.0;
D. adopt external standard method, utilize calculated by peak area to go out the content of shikimic acid.
Most preferably, shikimic acid content assaying method in a kind of shengmai injection of the present invention, implement by the following technical programs:
A. get shengmai injection sample, after diluting 20 times, as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm, and 4micron, detects wavelength: 218nm; Column temperature: 30 DEG C; Flow velocity: 0.6ml/mim; The KH of mobile phase: 20mM/L
2pO
4solution (H
3pO
4adjust pH to 2.5);
D. adopt external standard method, utilize calculated by peak area to go out the content of shikimic acid.
In shengmai injection of the present invention, in shikimic acid, content must not be lower than 1.2mg/ml.
The preparation method of the application's shengmai injection is as follows:
A. according to the following weight parts material of getting it filled: 25 parts of red ginsengs, 39 parts, the fruit of Chinese magnoliavine, 78 parts of the tubers of dwarf lilyturf;
B. red ginseng extracts: get red ginseng shave or pulverizing, add ethanol and carry out continuously or gradation refluxing extraction, merge extract, refrigeration, filters, suitably concentration and recovery ethanol, add proper amount of active carbon, agitation and filtration, filtrate regulates pH value near neutral, leave standstill, filtrate is concentrated into thick paste, injects water, stir evenly, refrigeration, filters, add proper amount of active carbon to boil, filter, to be prepared;
C. fruit of Chinese magnoliavine distillate: get the fruit of Chinese magnoliavine, the distillation that adds water after pulverizing, collects distillate, and refrigeration is to be prepared;
D. Fructus Schisandrae Chinensis extractive solution: the dregs of a decoction boiling after distillation, merge decoction liquor, filter, filtrate is concentrated into thick paste, adds ethanol to carry out secondary alcohol precipitation, and filtrate regulates pH value near neutral, refrigeration, filters, and reclaims ethanol and is concentrated into thick paste, add water for injection, stir, refrigeration, filter, filtrate adds proper amount of active carbon to boil 30 minutes, slightly cold, be filtered to clear and bright, to be prepared;
E. extract the tuber of dwarf lilyturf: get the tuber of dwarf lilyturf, boiling, the method for rear same Fructus Schisandrae Chinensis extractive solution, to be prepared;
F. get above-mentioned red ginseng extract, Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extractive solution, fruit of Chinese magnoliavine distillate,, mixing, heating, leaves standstill, filter, add polyoxyethylene sorbitan monoleate, mix, it is dissolved completely, moisturizing, stirs evenly, adjust pH, filters, embedding, and sterilizing, obtains shengmai injection.
Preferably, the preparation method of the application's shengmai injection is as follows:
A. according to the following weight parts material of getting it filled: 25 parts of red ginsengs, 39 parts, the fruit of Chinese magnoliavine, 78 parts of the tubers of dwarf lilyturf;
B. red ginseng extracts: get red ginseng sheet and add in refluxing extraction tank, and 90% above alcohol heating reflux extraction 4-5 time, each 2 hours, or continuous circumfluence extraction 6.5 hours, merging phegma, refrigeration, filters, and recovery ethanol extremely every 1ml contains crude drug 0.3～0.4g, add proper amount of active carbon, agitation and filtration, filtrate regulates pH value near neutral, leave standstill, filtrate is concentrated into thick paste, injects water, stir evenly, refrigeration, filters, add proper amount of active carbon to boil, filter, to be prepared;
C. fruit of Chinese magnoliavine distillation: get the fruit of Chinese magnoliavine, the distillation that adds water after pulverizing, collects distillate, and refrigeration is to be prepared;
D. Fructus Schisandrae Chinensis extractive solution: the dregs of a decoction boiling after distillation, merge decoction liquor, filter, filtrate is concentrated into thick paste, add ethanol to carry out secondary alcohol precipitation, make for the first time to reach 80% containing alcohol amount, make for the second time to reach 85% containing alcohol amount, filtrate regulates pH value near neutral, and refrigeration, filters, reclaim ethanol and be concentrated into thick paste, adding water for injection, stirring, refrigeration, filters, and filtrate adds proper amount of active carbon to boil 30 minutes, slightly cold, be filtered to clear and bright, to be prepared;
E. extract the tuber of dwarf lilyturf: get the tuber of dwarf lilyturf, boiling, rear same Fructus Schisandrae Chinensis extractive solution method;
F. get red ginseng extract, Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extractive solution, fruit of Chinese magnoliavine distillate, respectively ultrafiltration again after initial filter, mix filtrate, add 0.5-0.9% polyoxyethylene sorbitan monoleate, it is dissolved completely, moisturizing, to the volume of 1.5 ~ 2.0 times of medicinal material three taste medicinal material total amounts, stirs evenly adjust pH 7.5.Essence filter, embedding, sterilizing.
The assay method of shikimic acid in the application's shengmai injection, specificity is strong, and in the concentration range of 4-128 μ g/ml, linear good, the method can be for the assay of shikimic acid in shengmai injection.
Below, by methodological study, beneficial effect of the present invention is described.
1, specificity research
According to the method for embodiment 1, get red ginseng parenteral solution, the tuber of dwarf lilyturf parenteral solution and tween of shengmai injection in producing, be not made into containing the negative sample of the raw shengmai injection of fruit of Chinese magnoliavine liquid by configuration proportion.By shengmai injection and scarce fruit of Chinese magnoliavine liquid feminine gender with after 50 times of water for injection dilutions, by method sample introduction.A: shikimic acid reference substance; B: shengmai injection; C: lack fruit of Chinese magnoliavine liquid feminine gender, various kinds is all entered 5 μ l.The results are shown in Figure 1-Fig. 3;
2, detect determining of wavelength
It is appropriate that precision takes shikimic acid reference substance, with the KH of 2m mol/L
2pO
4, H
3pO
4the aqueous solution of adjusting pH=2.5, is diluted to the reference substance solution that content is about 40 μ g/ml.Get reference substance solution 190-400nm length scanning, obtain shikimic acid ultraviolet spectrogram, see Fig. 4.Can find out, shikimic acid has absorption at 190-250nm, and obtained the maximum absorption is at 218nm;
3, chromatographic condition
Chromatographic column: Synergi 4u Hydro-RP 80A, 250 x 4.6 mm; Detect wavelength: 218nm; Column temperature: 25 DEG C; Mobile phase: single pump, the KH of 20mM/L
2pO
4(H
3pO
4adjust pH to 2.5); Flow velocity: 0.6ml/mim; Sample size: 10 μ L; Must not be lower than 15000 in the shikimic acid number of plates;
4 repeated experiments
4.1 withinday precision
Method according to embodiment 1 is prepared sample, gets shengmai injection sample, after diluting 50 times, and sample introduction 5 μ l, continuous sample introduction 6 times, gained shikimic acid peak calculated by peak area RSD=0.12%, in table 1.Illustrate that this method is good for measuring shikimic acid withinday precision;
Table 1, Precision Experiment Timeing Detection Table
4.2 day to day precision
Method according to embodiment 1 is prepared sample, gets 5, same lot number shengmai injection sample, divides and within 5 days, detects its shikimic acid content, calculates RSD=1.49% of its content.In table 2, illustrate that this method is good for precision in the middle of measuring shikimic acid;
Table 2, Precision Experiment Timeing Detection Table
5 linearities and sensing range
Take shikimic acid reference substance appropriate, be made into every milliliter of reference substance storing solution containing shikimic acid 200 μ g/ml.Get appropriate storing solution and be made into the titer that content is 4 μ g/ml, 8 μ g/ml, 16 μ g/ml, 32 μ g/ml, 64 μ g/ml, 128 μ g/ml.Titer is pressed to aforementioned chromatographic process sample introduction respectively.Least square method linearity of regression is in table 3;
Table 3, regression beeline equation
| Straight-line equation | R 2 |
| A = 0.1486C+0.1975 | 0.9996 |
6 detectabilities and quantitative limit
By the dilution of shikimic acid reference substance, detect by above-mentioned potassium dihydrogen phosphate chromatographic process, in the time that sample introduction concentration is 0.35 μ g/ml, reach minimum quantitative limit.By the dilution of shikimic acid reference substance, detect by potassium hydrogen phosphate chromatographic process, in the time that sample introduction concentration is 0.1 μ g/ml, reach lowest detectable limit;
7 accuracy
Method according to embodiment 1 is prepared sample, gets 1 batch of shengmai injection (shikimic acid assay is 1.59mg/ml), dilutes 50 times, does for sample.Get shikimic acid titer (32 μ g/ml), prepare with supplying sample 1:1 volume, prepare altogether 6 groups of samples, to be measured.Calculating shikimic acid recovery mean value is 101.5, RSD=1.39%.In table 4;
Table 4, shikimic acid assay average recovery experimental result
8 brief summaries
With the KH of 20mM/L
2pO
4(H
3pO
4adjust pH to 2.5) solution makes mobile phase, under applicable uv absorption wavelength, can carry out quantitatively shikimic acid composition in shengmai injection accurately, and in the concentration range of 4-128 μ g/ml, linear good, the method can be for the assay of shikimic acid in shengmai injection.
Brief description of the drawings
Fig. 1 is that shikimic acid is at shengmai injection special secondary school attributes research collection of illustrative plates (shikimic acid reference substance).
Fig. 2 is that shikimic acid is at shengmai injection special secondary school attributes research collection of illustrative plates (shengmai injection).
Fig. 3 is that shikimic acid is at shengmai injection special secondary school attributes research collection of illustrative plates (lacking fruit of Chinese magnoliavine negative control product).
Fig. 4 is shikimic acid ultraviolet scanning spectrum.
Specific embodiment
The preparation of embodiment 1(shengmai injection)
[prescription] red ginseng 100g 312g tuber of dwarf lilyturf fruit of Chinese magnoliavine 156g
[method for making] above three tastes, red ginseng shave or pulverizing, by heating and refluxing extraction after 90% above alcohol dipping 4～5 times, each 2 hours; Or continuous circumfluence extraction 6.5 hours, merge extract, refrigeration, filter, recovery ethanol containing crude drug 0.3～0.4g, adds proper amount of active carbon to every 1ml, stir, filtrate adjust pH, near neutral, leaves standstill, filtrate is concentrated into thick paste shape, adds water for injection to 200ml, stirs evenly, refrigeration, filters, and adds proper amount of active carbon and boils, filter, filtrate is for dosing; The fruit of Chinese magnoliavine, pulverizes if desired, with steam distillation collection distillate 150ml, refrigeration, for dosing, the dregs of a decoction are boiling secondary again, each 40 minutes, merge decoction liquor, filter, filtrate is concentrated into thick paste shape, adds ethanol to carry out secondary alcohol precipitation, makes for the first time to reach 80% containing alcohol amount, make for the second time to reach 85% containing alcohol amount, regulate pH value near neutral, refrigeration, filter, reclaim ethanol and be concentrated into thick paste shape, adding water for injection to 150ml, stirring evenly, refrigeration, filter, filtrate adds proper amount of active carbon boils 30 minutes, slightly cold, be filtered to clear and bright, for dosing; Make clear and bright tuber of dwarf lilyturf aqueous solution 200ml according to the fruit of Chinese magnoliavine water liquid and preparation method thereof tuber of dwarf lilyturf, for dosing.By above-mentioned red ginseng water liquid, fruit of Chinese magnoliavine distillate, fruit of Chinese magnoliavine water liquid and the water liquid tuber of dwarf lilyturf (mix, heating, leaves standstill, and filters), add 3～5g polyoxyethylene sorbitan monoleate, mix, filter, filtrate injects water to 1000ml, regulates liquid pH value to 7.5, filter, embedding, sterilizing, to obtain final product).
Embodiment 2(shikimic acid assay)
A. get shengmai injection sample, suitably dilute after 20 times, as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm, and 4micron, detects wavelength: 218nm; Column temperature: 30 DEG C; Flow velocity: 0.6ml/mim; The KH of mobile phase: 20mM/L
2pO
4solution (H
3pO
4adjust pH to 2.5);
D. the content of shikimic acid in external standard method shengmai injection.
Embodiment 3(shikimic acid assay)
A. get shengmai injection sample, suitably dilute after 20 times, as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm, and 4micron, detects wavelength: 218nm; Column temperature: 30 DEG C; Flow velocity: 0.6ml/mim; The KH of mobile phase: 15mM/L
2pO
4solution (H
3pO
4adjust pH to 2.5);
D. the content of shikimic acid in external standard method shengmai injection.
Embodiment 4(shikimic acid assay)
A. get shengmai injection sample, suitably dilute after 20 times, as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm, and 4micron, detects wavelength: 218nm; Column temperature: 30 DEG C; Flow velocity: 0.6ml/mim; The KH of mobile phase: 25mM/L
2pO
4solution (H
3pO
4adjust pH to 2.5);
D. the content of shikimic acid in external standard method shengmai injection.
Claims (7)
1. an assay method for shikimic acid in shengmai injection, is made up of following step:
A. get shengmai injection sample, suitably after dilution as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm 4micron, detect wavelength: 200-230nm; Column temperature: 25-40 DEG C; Flow velocity: 0.5-0.7ml/mim; The KH of mobile phase: 15 ~ 25mM/L
2pO
4solution, H
3pO
4adjust pH to 2.0 ~ 3.0;
D. adopt external standard method, utilize calculated by peak area to go out the content of shikimic acid.
2. method claimed in claim 1, wherein in step a, dilution is 20-50 times.
3. method claimed in claim 1, wherein in step c, mobile phase is: the KH of 15 ~ 25mM/L
2pO
4solution, H
3pO
4adjust pH to 2.0 ~ 3.0.
4. method claimed in claim 1, is characterized in that being made up of following step:
A. get shengmai injection sample, dilute after 20-50 times, as test sample;
B. get shikimic acid reference substance, preparation is into about 80 μ g/ml;
C. carry out high-performance liquid chromatogram determination, wherein chromatographic condition is, chromatographic column: Phenomenex Synergi 4 μ m Hydro-RP 80A 250 × 4.60mm, and 4micron, detects wavelength: 200-230nm; Column temperature: 25-40 DEG C; Flow velocity: 0.5-0.7ml/mim; The KH of mobile phase: 15 ~ 25mM/L
2pO
4solution, H
3pO
4adjust pH to 2.0 ~ 3.0;
D. adopt external standard method, utilize calculated by peak area to go out the content of shikimic acid.
5. method claimed in claim 1, wherein shengmai injection is made according to following step:
A. according to the following weight parts material of getting it filled: 25 parts of red ginsengs, 39 parts, the fruit of Chinese magnoliavine, 78 parts of the tubers of dwarf lilyturf;
B. red ginseng extracts: get red ginseng shave or pulverizing, add ethanol and carry out continuously or gradation refluxing extraction, merge extract, refrigeration, filters, suitably concentration and recovery ethanol, add proper amount of active carbon, agitation and filtration, filtrate regulates pH value near neutral, leave standstill, filtrate is concentrated into thick paste, injects water, stir evenly, refrigeration, filters, add proper amount of active carbon to boil, filter, to be prepared;
C. fruit of Chinese magnoliavine distillate: get the fruit of Chinese magnoliavine, the distillation that adds water after pulverizing, collects distillate, and refrigeration is to be prepared;
D. Fructus Schisandrae Chinensis extractive solution: the dregs of a decoction boiling after distillation, merge decoction liquor, filter, filtrate is concentrated into thick paste, adds ethanol to carry out secondary alcohol precipitation, and filtrate regulates pH value near neutral, refrigeration, filters, and reclaims ethanol and is concentrated into thick paste, add water for injection, stir, refrigeration, filter, filtrate adds proper amount of active carbon to boil 30 minutes, slightly cold, be filtered to clear and bright, to be prepared;
E. extract the tuber of dwarf lilyturf: get the tuber of dwarf lilyturf, boiling, the method for rear same Fructus Schisandrae Chinensis extractive solution, to be prepared;
F. get above-mentioned red ginseng extract, Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extractive solution, fruit of Chinese magnoliavine distillate, mixes, and heating leaves standstill, and filters, add polyoxyethylene sorbitan monoleate, mix, it is dissolved completely, moisturizing, stirs evenly, adjust pH, filters, embedding, and sterilizing, obtains shengmai injection.
6. the method for claim 5, is characterized in that, shengmai injection is made up of following step:
A. according to the following weight parts material of getting it filled: 25 parts of red ginsengs, 39 parts, the fruit of Chinese magnoliavine, 78 parts of the tubers of dwarf lilyturf;
B. red ginseng extracts: get red ginseng sheet and add in refluxing extraction tank, and 90% above alcohol heating reflux extraction 4-5 time, each 2 hours, or continuous circumfluence extraction 6.5 hours, merging phegma, refrigeration, filters, and recovery ethanol extremely every 1ml contains crude drug 0.3～0.4g, add proper amount of active carbon, agitation and filtration, filtrate regulates pH value near neutral, leave standstill, filtrate is concentrated into thick paste, injects water, stir evenly, refrigeration, filters, add proper amount of active carbon to boil, filter, to be prepared;
C. fruit of Chinese magnoliavine distillation: get the fruit of Chinese magnoliavine, the distillation that adds water after pulverizing, collects distillate, and refrigeration is to be prepared;
D. Fructus Schisandrae Chinensis extractive solution: the dregs of a decoction boiling after distillation, merge decoction liquor, filter, filtrate is concentrated into thick paste, add ethanol to carry out secondary alcohol precipitation, make for the first time to reach 80% containing alcohol amount, make for the second time to reach 85% containing alcohol amount, filtrate regulates pH value near neutral, and refrigeration, filters, reclaim ethanol and be concentrated into thick paste, adding water for injection, stirring, refrigeration, filters, and filtrate adds proper amount of active carbon to boil 30 minutes, slightly cold, be filtered to clear and bright, to be prepared;
E. extract the tuber of dwarf lilyturf: get the tuber of dwarf lilyturf, boiling, rear same Fructus Schisandrae Chinensis extractive solution method;
F. get red ginseng extract, Radix Ophiopogonis extract, Fructus Schisandrae Chinensis extractive solution, fruit of Chinese magnoliavine distillate, respectively ultrafiltration again after initial filter, mix filtrate, add 0.5-0.9% polyoxyethylene sorbitan monoleate, it is dissolved completely, moisturizing, to the volume of 1.5 ~ 2.0 times of medicinal material three taste medicinal material total amounts, stirs evenly adjust pH 7.5; Essence filter, embedding, sterilizing.
7. method claimed in claim 1, wherein in shengmai injection in shikimic acid, content must not be lower than 1.2mg/ml.
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| CN105116092A (en) * | 2015-09-06 | 2015-12-02 | 山东省果树研究所 | HPLC determination method for shikimic acid and 3-dehydrogenated shikimic acid in pomegranate peels |
| CN108195988A (en) * | 2018-02-01 | 2018-06-22 | 华润三九（雅安）药业有限公司 | Using the schisandra chinensis medicinal material of the construction method of HPLC finger-prints and its pulse invigorating injection liquid formulation |
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