CN103834632B - The liquid Bromenzyme that a kind of enzyme activity is stable - Google Patents
The liquid Bromenzyme that a kind of enzyme activity is stable Download PDFInfo
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- CN103834632B CN103834632B CN201310705809.XA CN201310705809A CN103834632B CN 103834632 B CN103834632 B CN 103834632B CN 201310705809 A CN201310705809 A CN 201310705809A CN 103834632 B CN103834632 B CN 103834632B
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- enzyme activity
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- bromenzyme
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- bromeline
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
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Abstract
The present invention relates to a kind of liquid bromeline, particularly relate to the liquid Bromenzyme that a kind of enzyme activity is stable.The liquid Bromenzyme that a kind of enzyme activity disclosed by the invention is stable, is made up of bromelain enzyme aqueous solution, sugar alcohol, monosaccharide and disaccharide, oligofructose.With the liquid Bromenzyme of above-mentioned formulated, store 12 months at normal temperatures, enzyme activity still preserves more than 95%, have that enzyme activity stability is high, equipment investment is few, energy-conservation, joint time, movement requirement is low, user is easy to use, be easy to the feature of enhancing production capacities.
Description
Technical field
The present invention relates to a kind of liquid bromeline, particularly relate to the liquid Bromenzyme that a kind of enzyme activity is stable.
Background technology
Bromeline is the proteolytic enzyme being present in pineapple plant (comprising fruit, stem, handle and blade), and sulfydryl (-SH) is contained in its active centre, is widely used in the industries such as medicine, food, makeup, weaving, feed, leather.
The extracting and purifying method of protein mainly contain saltout, the method such as isoelectric precipitation, organic solvent deposit, tannin (tannic acid) precipitation, ion exchange chromatography, affinity chromatography, ultrafiltration and concentration.The production technique of bromeline mainly contains ammonium sulfate precipitation method, the tannin precipitator method, ultrafiltration process etc., and last step of these methods is all drying process, and dry method mainly contains lyophilize and vacuum and heating drying.Compared with vacuum and heating drying, the solvability of lyophilize products obtained therefrom is good, and enzymatic activity recovery is high.But lyophilize needs freeze drier, equipment investment is high, power consumption, consuming time, production capacity also not easily improves, and in addition, in freeze-drying process, the loss of enzyme activity still reaches 10% ~ 30%.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, the object of the present invention is to provide the liquid bromeline that a kind of enzyme activity is stable, to avoid the drying process of enzyme in bromeline production process, reach when reducing equipment investment, energy-conservation, joint, be user-friendly to, be easy to the object of enhancing production capacities.
Object of the present invention is achieved through the following technical solutions:
The liquid Bromenzyme that enzyme activity is stable, is characterized in that: it is made up of the component of following weight percent:
Preferably: described sugar alcohol is sorbyl alcohol or N.F,USP MANNITOL, monose is glucose or fructose, and disaccharide is sucrose, and oligofructose is kestose.
Preferably: in bromelain enzyme aqueous solution, bromeline content is 1 ~ 5%.
Preferably: described sugar alcohol, monosaccharide and disaccharide, oligofructose are food grade or pharmaceutical grade.
Preferably: the stable liquid Bromenzyme storage requirement of described enzyme activity is 0 ~ 35 DEG C, and best storage temperature is 4 DEG C.
Preferably: after the liquid bromeline preparation that described enzyme activity is stable, regulate pH, pH to be 3 ~ 5 with 2mol/L citric acid solution.
With the liquid bromeline of above-mentioned formulated, store 12 months at normal temperatures, enzyme activity still preserves more than 95%, have that enzyme activity stability is high, equipment investment is few, energy-conservation, joint time, movement requirement is low, user is easy to use, be easy to the feature of enhancing production capacities.
Embodiment
Below in conjunction with specific embodiment, elaboration detailed is further done to the present invention, but embodiments of the present invention are not limited to the scope that embodiment represents.These embodiments only for illustration of the present invention, but not for limiting the scope of the invention.In addition, after reading content of the present invention, those skilled in the art can do various amendment to the present invention, and these equivalent variations fall within appended claims limited range of the present invention equally.
Embodiment 1
In 200 grams of Traumanase aqueous solution (bromeline content is 4%) of preliminary purification, concentrated, filtration sterilization, adding 600 grams of sorbyl alcohols respectively, 50 grams of glucose, 100 grams of sucrose, 50 grams of kestoses, described sorbyl alcohol, glucose, sucrose, kestose are food grade or pharmaceutical grade.Under room temperature (25 DEG C), stir (100r/min) 60 minutes, wait that added enzyme activity protective material all dissolves, adjust pH to 3 with citric acid solution (2mol/L), in 2 hours, measure enzyme activity.Measure enzyme activity after depositing 6 months in 0 DEG C, enzyme activity declines 1.8%; Measure enzyme activity after continuing to deposit 6 months in 0 DEG C, enzyme activity declines 3.9%.
Embodiment 2
In 230 grams of Traumanase aqueous solution (bromeline content is 5%) of preliminary purification, concentrated, filtration sterilization, adding 520 grams of sorbyl alcohols respectively, 150 grams of glucose, 50 grams of sucrose, 50 grams of kestoses, described sorbyl alcohol, glucose, sucrose, kestose are food grade or pharmaceutical grade.Under room temperature (25 DEG C), stir (90r/min) 60 minutes, wait that added enzyme activity protective material all dissolves, adjust pH to 3.5 with citric acid solution (2mol/L), in 2 hours, measure enzyme activity.Measure enzyme activity after depositing 6 months in 35 DEG C, enzyme activity declines 2.3%; Measure enzyme activity after continuing to deposit 6 months in 35 DEG C, enzyme activity declines 4.8%.
Embodiment 3
In 250 grams of Traumanase aqueous solution (bromeline content is 2%) of preliminary purification, concentrated, filtration sterilization, adding 500 grams of sorbyl alcohols respectively, 150 grams of glucose, 50 grams of sucrose, 50 grams of kestoses, described sorbyl alcohol, glucose, sucrose, kestose are food grade or pharmaceutical grade.Under room temperature (25 DEG C), stir (90r/min) 60 minutes, wait that added enzyme activity protective material all dissolves, adjust pH to 5 with citric acid solution (2mol/L), in 2 hours, measure enzyme activity.Measure enzyme activity after depositing 6 months in 4 DEG C, enzyme activity declines 1.3%; Measure enzyme activity after continuing to deposit 6 months in 4 DEG C, enzyme activity declines 3.5%.
Embodiment 4
In 200 grams of Traumanase aqueous solution (bromeline content is 3%) of preliminary purification, concentrated, filtration sterilization, adding 450 grams of N.F,USP MANNITOL respectively, 100 grams of fructose, 150 grams of sucrose, 100 grams of kestoses, described N.F,USP MANNITOL, fructose, sucrose, kestose are food grade or pharmaceutical grade.Under room temperature (25 DEG C), stir (90r/min) 60 minutes, wait that added enzyme activity protective material all dissolves, adjust pH to 5 with citric acid solution (2mol/L), in 2 hours, measure enzyme activity.Measure enzyme activity after depositing 6 months in 10 ~ 29 DEG C, enzyme activity declines 2.1%; Measure enzyme activity after continuing to deposit 6 months in 4 DEG C, enzyme activity declines 4.1%.
Through preparing liquid bromeline with aforesaid method, enzyme activity stability is high, in all only loss less than 5% of 12 months inner enzyme vigors, and have that equipment investment is few, energy-conservation, joint time, simple to operate, be easy to the feature expanded.
Claims (2)
1. the liquid Bromenzyme that enzyme activity is stable, is characterized in that: it is made up of the component of following weight percent:
Described sugar alcohol is sorbyl alcohol or N.F,USP MANNITOL, and monose is glucose or fructose, and disaccharide is sucrose, and oligofructose is kestose;
In bromelain enzyme aqueous solution, bromeline content is 1-5%;
Sugar alcohol used, monosaccharide and disaccharide, oligofructose are food grade or pharmaceutical grade;
The stable liquid Bromenzyme storage requirement of described enzyme activity is 0-35 DEG C;
PH is regulated to be 3-5 with 2mol/L citric acid solution after the liquid bromeline preparation that described enzyme activity is stable.
2. the liquid Bromenzyme that enzyme activity according to claim 1 is stable, is characterized in that: the stable liquid Bromenzyme storage requirement of described enzyme activity is 4 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310705809.XA CN103834632B (en) | 2013-12-19 | 2013-12-19 | The liquid Bromenzyme that a kind of enzyme activity is stable |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310705809.XA CN103834632B (en) | 2013-12-19 | 2013-12-19 | The liquid Bromenzyme that a kind of enzyme activity is stable |
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| Publication Number | Publication Date |
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| CN103834632A CN103834632A (en) | 2014-06-04 |
| CN103834632B true CN103834632B (en) | 2015-11-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310705809.XA Expired - Fee Related CN103834632B (en) | 2013-12-19 | 2013-12-19 | The liquid Bromenzyme that a kind of enzyme activity is stable |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113197271A (en) * | 2021-06-08 | 2021-08-03 | 西南大学 | Solid beverage with digestion promoting function |
| CN114699517B (en) * | 2022-04-25 | 2023-04-14 | 南京鲸力医药科技有限公司 | Bromelain preparation and application thereof in detumescence composition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0821058A2 (en) * | 1996-07-25 | 1998-01-28 | The Institute Of Physical & Chemical Research | Method for enhancing enzyme activity at elevated temperature |
| CN101173264A (en) * | 2007-10-31 | 2008-05-07 | 天津大学 | Beta-mannase composite preparations and method for producing the same |
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2013
- 2013-12-19 CN CN201310705809.XA patent/CN103834632B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0821058A2 (en) * | 1996-07-25 | 1998-01-28 | The Institute Of Physical & Chemical Research | Method for enhancing enzyme activity at elevated temperature |
| CN101173264A (en) * | 2007-10-31 | 2008-05-07 | 天津大学 | Beta-mannase composite preparations and method for producing the same |
Non-Patent Citations (1)
| Title |
|---|
| 不同添加物对菠萝蛋白酶活稳定性和抑制作用的影响;薛力荔 等;《肉类研究》;20110430;第25卷(第4期);第7-11页 * |
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| CN103834632A (en) | 2014-06-04 |
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Granted publication date: 20151118 Termination date: 20161219 |