CN103830380A - Chinese medicinal composition for treating diabetic nephropathy and preparation method thereof - Google Patents
Chinese medicinal composition for treating diabetic nephropathy and preparation method thereof Download PDFInfo
- Publication number
- CN103830380A CN103830380A CN201410133378.9A CN201410133378A CN103830380A CN 103830380 A CN103830380 A CN 103830380A CN 201410133378 A CN201410133378 A CN 201410133378A CN 103830380 A CN103830380 A CN 103830380A
- Authority
- CN
- China
- Prior art keywords
- parts
- radix
- chinese medicine
- relative density
- achyranthis asperae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a Chinese medicinal composition for treating diabetic nephropathy. The Chinese medicinal composition is prepared from the following Chinese medicinal decoction pieces in parts by weight: 15-25 parts of astragalus, 7-13 parts of cherokee rose fruit, 7-13 parts of Szechuan lovage rhizome, 10-20 parts of sunset abelmoschus flower, 2-8 parts of rhubarb, 7-13 parts of kudzuvine root and 10-20 parts of common achyranthes herb. An oral preparation of the traditional Chinese medicine is prepared by the following steps: extracting the astragalus, the Szechuan lovage rhizome, the sunset abelmoschus flower and the kudzuvine root by using ethanol; collecting an extracting solution and concentrating into clear paste of which the relative density is 1.08-1.15; extracting together with the cherokee rose fruit, the rhubarb and the common achyranthes herb by using water; collecting an extracting solution and concentrating to clear paste of which the relative density is 1.08-1.5; mixing the two clear pastes; continually concentrating into thick paste of which the relative density is 1.30-1.40; adding auxiliary materials to obtain a dosage form suitable for clinical application. A prepared Chinese medicinal oral preparation has the advantages of high extraction efficiency of effective components, stable content, good medicinal effect, low dosage and easiness in preparation and formulation.
Description
Technical field
The present invention relates to Chinese medicine, be specifically related to a kind of Chinese medicine composition for the treatment of diabetic nephropathy and preparation method thereof.
Background technology
Diabetic nephropathy (diabetic nephropathy, DN) is one of chronic complicating diseases that diabetes are common and serious, is also diabetics major causes of death.Diabetes continue 10～20 years, it is reported that the sickness rate of DN is about 40～50% in type 1 diabetes, and in type 2 diabetes mellitus, DN incidence rate is about 30～40%.DN is the main microvascular complications of diabetes, mainly refers to diabetic glomerulosclerosis, a kind of taking vascular lesion as main Glomerular lesions.In early days how asymptomatic, its incidence rate is along with the course of disease of diabetes extends and increases.The early stage kidney volume of diabetes increases, glomerular filtration rate increases, and is high filtration state, engenders discontinuity albuminuria or microalbuminuria later, along with appearring continuing in the prolongation of the course of disease, albuminuria, edema, hypertension, glomerular filtration rate reduce, and then renal insufficiency, uremia.Diabetic nephropathy has been most important reason in whole nephropathy in latter stage (ESRF), also be the one of the main reasons that diabetics is lethal, this has been carried out to a large amount of research both at home and abroad, but pathogenesis is illustrated not yet completely so far, be there is no effective medicine and stop it to occur and worsen.
From traditional Chinese medical science angle, the normalized names of disease of tcm of diabetic nephropathy is the nephropathy of quenching one's thirst, and with the passing of time, the kidneys such as the caused turbid urine of pathogen usually intruding into collateral in protracted disease, edema, pain in the lumbar region, obstruction and rejection are complication to diabetes, and sick position, at kidney, is secondary to diabetes.
From doctor trained in Western medicine angle, the basic pathology feature of diabetic nephropathy is that proliferation of mesangial cells, ectoplasm increase, glomerular sclerosis and basement membrane thickened etc.Its definite pathogenesis is not yet illustrated, now generally believe that the interior three large substance metabolism disorders of body, renal hemodynamics change that long-term hyperglycemia causes are DN pathological changes bases, it is the direct factor that causes DN that cytokine is activated, and oxidative stress is the Common Mechanism that DN occurs.Clinical manifestation is mainly glomerular filtration rate increases, albuminuria, hypertension, renal insufficiency (albuminuria appearance, serum BUN, Scr content obviously raise) etc.Current clinical main bases is mainly the detection of urine protein.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine composition and Chinese medicine preparation for the treatment of diabetic nephropathy, another object of the present invention is to provide a kind of preparation method for the treatment of diabetic nephropathy Chinese medicine composition.
For achieving the above object, the present invention is by the following technical solutions:
Treat a Chinese medicine composition for diabetic nephropathy, this Chinese medicine composition is to be made up of the prepared slices of Chinese crude drugs of following weight proportioning: 15～25 parts of the Radixs Astragali, 7～13 parts of Fructus Rosae Laevigatae, 7～13 parts of Rhizoma Chuanxiongs, 10～20 parts of Flos abelmoschi manihots, 2～8 parts of Radix Et Rhizoma Rhei, 7～13 parts of Radix Puerariaes, 10～20 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s.
The Chinese medicine composition for the treatment of diabetic nephropathy as above, preferably, described Chinese medicine composition is to be made up of the prepared slices of Chinese crude drugs of following weight proportioning: 20 parts of the Radixs Astragali, 10 parts of Fructus Rosae Laevigatae, 10 parts of Rhizoma Chuanxiongs, 15 parts of Flos abelmoschi manihots, 5 parts of Radix Et Rhizoma Rhei, 15 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 10 parts of Radix Puerariaes.
A kind of preparation method for the treatment of diabetic nephropathy Chinese medicine composition, the method comprises: take the prepared slices of Chinese crude drugs by weight proportion as above, the Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae are added by weight to 50～80 volume % alcohol reflux 1～4 time of 8～14 times, each 0.5～3 hour, collecting extracting solution and being concentrated into relative density was 1.08～1.15 clear paste; Fructus Rosae Laevigatae, Radix Et Rhizoma Rhei, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 1～4 time of 8～12 times, each 0.5～2 hour, collecting extracting solution and being concentrated into relative density was 1.08～1.15 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.30～1.40 thick paste.
Preparation method as above, preferably, described method comprises: take the prepared slices of Chinese crude drugs by weight proportion as above, the Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae are added by weight to 63% alcohol reflux 2 times of 12 times, each 2.5 hours, collecting extracting solution and being concentrated into relative density was 1.1 clear paste; Fructus Rosae Laevigatae, Radix Et Rhizoma Rhei, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 3 times of 10 times, each 1.5 hours, collecting extracting solution and being concentrated into relative density was 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste.
Treat a Chinese medicine composition for diabetic nephropathy, it is prepared according to method described above.
Treat an oral preparation of Chinese traditional medicinal for diabetic nephropathy, it is made up of with adjuvant Chinese medicine composition as above and medicinal or food.
Oral preparation of Chinese traditional medicinal as above, preferably, described oral formulations is oral liquid, tablet, granule or capsule.
Treatment diabetic nephropathy oral preparation of Chinese traditional medicinal as above, preferably, described oral formulations is granule, and this granule is made up of the material of following weight proportioning: 1～5 part of 400～600 parts of described thick pastes, 200～300 parts, dextrin, 200～300 parts, mannitol and aspartame.
Treatment diabetic nephropathy oral preparation of Chinese traditional medicinal as above, preferably, described granule is made up of the material of following weight parts: 2.5 parts of 500 parts of described thick pastes, 250 parts, dextrin, 250 parts, mannitol and aspartames.
The detection method of Chinese medicine composition as mentioned above, preferably, ethanol extraction part is taking the content of astragaloside, hyperin, puerarin as testing index, minima is respectively 0.8214mg/g, 26.3417mg/g, 5.4362mg/g, water extraction part is taking the content of chrysophanic acid as testing index, and minima is 2.5141mg/g.
A kind of method of simultaneously measuring hyperin and puerarin content in Chinese medicine composition described above, the method is that detected sample is measured by high performance liquid chromatography, the mobile phase of this high performance liquid chromatography is acetonitrile and 0.05%～0.3%(volumn concentration) the mixed liquor of phosphate aqueous solution composition, the volume ratio of acetonitrile and phosphate aqueous solution is (10～30): (90～70); The chromatographic column that high performance liquid chromatography adopts is C
18, the flow velocity of mobile phase is (0.8～1.2) mL/min; Detect wavelength: puerarin is 250nm, and hyperin is 360nm, and sample size is 8～12 μ l.
Method as above, preferably, the method is that detected sample is measured by high performance liquid chromatography, and the mobile phase of this high performance liquid chromatography is the mixed liquor of acetonitrile and the volumn concentration phosphate aqueous solution composition that is 0.1%, and the volume ratio of acetonitrile and phosphate aqueous solution is 15:85; The chromatographic column of high performance liquid chromatography is C
18, flow rate of mobile phase is 1.0mL/min; Detect wavelength: puerarin is 250nm, and hyperin is 360nm, and sample size is 10 μ l.
A kind of method of measuring Rhein Content in Chinese medicine composition described above, the method is that detected sample is measured by high performance liquid chromatography, the mobile phase of high performance liquid chromatography is the mixed liquor of methanol and the volumn concentration phosphate aqueous solution composition that is 0.05～0.3%, and the percent by volume of methanol in mixed liquor is 60～85%; The chromatographic column of high performance liquid chromatography is C
18, flow rate of mobile phase is 0.8～1.2mL/min; Detection wavelength is 436nm, and sample size is 8～12 μ l.
Method as above, preferably, the method is that detected sample is measured by high performance liquid chromatography, and the mobile phase of high performance liquid chromatography is the mixed liquor of methanol and the volumn concentration phosphate aqueous solution composition that is 0.1%, and the volume ratio of methanol and phosphate aqueous solution is 75:25; The chromatographic column of high performance liquid chromatography is C
18, flow rate of mobile phase is 1.0mL/min; Detection wavelength is 436nm, and sample size is 10 μ l.
Beneficial effect of the present invention is: the Chinese medicine composition for the treatment of diabetic nephropathy provided by the invention has that QI invigorating is reinforced the kidney, effect of blood stasis dispelling the turbid descending, can obviously reduce DN Urinary albumen, improves renal function.Chinese medicine preparation prepared by the inventive method, extracts active ingredients efficiency is high, stable content, and the herbal medicine efficacy of preparing according to the present invention is good, and dose is low, and preparation molding is easy.
Brief description of the drawings
Fig. 1 is that in ethanol extraction extractum, astragaloside HPLC-ELSD measures collection of illustrative plates.
Fig. 2 is that in ethanol extraction extractum, puerarin HPLC-UV measures collection of illustrative plates.
Fig. 3 is that in ethanol extraction extractum, hyperin HPLC-UV measures collection of illustrative plates.
Fig. 4 is ethanol extraction part punctate opacity of the cornea design response surface contour map and 3D figure.
Fig. 5 be under pharmacopeia condition determination in water extraction extractum the HPLC-UV of Radix Et Rhizoma Rhei composition detect collection of illustrative plates.
Fig. 6 determines that Water Under extracts the HPLC-UV detection collection of illustrative plates of Radix Et Rhizoma Rhei composition in extractum.
Fig. 7 is the made granule sucting wet curve of single adjuvant.
Fig. 8 is that dextrin mixes made granule sucting wet curve with mannitol different proportion.
Fig. 9 is the made granule sucting wet curve of optimum process.
Detailed description of the invention
The present invention introduces the traditional Chinese medical science network ens morbi research of Chinese medicine prevention DN, thinks that network disease is the pathological state that is extensively present in the early, middle and late phase of diabetes nephropathy.Its pathogenic characteristic of DN is that kidney unit virtual loss, instability of kidney QI are this, stops as mark in kidney network stasis blocking, turbid poison.Kidney unit virtual loss, instability of kidney QI, opens entire mistake department, occurs edema, turbid urine, lumbago; Kidney network stasis blocking, stops in turbid poison, and contrary, generalized edema with distention of the chest and abdomen, obstruction and rejection appear telling in the three warmers that are jammed.For above-mentioned pathogenesis, the present invention adopts that QI invigorating is reinforced the kidney, the method for the treatment of of blood stasis dispelling the turbid descending is developed a kind of compound Chinese medicinal preparation.
Chinese medicine compound Raw medicine in the present invention adopts the Radix Astragali, Fructus Rosae Laevigatae, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Et Rhizoma Rhei, Radix Puerariae, Radix achyranthis asperae (Herba Achyranthis Asperae).
Wherein the Radix Astragali is monarch drug, sweet, tepor; Return lung, spleen, liver, kidney channel; Benefiting QI for strengthening the superficies, inducing diuresis to remove edema, can eliminate Jishengshenqiwan albuminuria, strengthens myocardial contraction, regulates blood sugar content, treatment chronic nephritis, albuminuria, diabetes etc.
Fructus Rosae Laevigatae is ministerial drug, and sweet, micro-acid, puckery is flat; Return spleen, kidney, large intestine, urinary bladder channel; Controlling nocturnal emission with astringent drugs reducing urination, increase serum urea and creatinine clearance rate reach the effect of renal function protecting.
Rhizoma Chuanxiong is ministerial drug, pungent, temperature; Return liver, gallbladder, heart channel; Blood-activating and qi-promoting blood stasis dispelling.Radix Et Rhizoma Rhei hardship, cold, clearing away damp-heat, removing heat from blood, blood stasis dispelling, removing toxic substances.Flos abelmoschi manihot is sweet, pungent, cool; Inducing diuresis for treating stranguria syndrome, invigorate blood circulation, hemostasis, subduing swelling and detoxicating.Two medicines are assistant, the merit of playing altogether the turbid descending.
Radix Puerariae, sweet, pungent, cool; Return spleen, stomach warp; Can be antipyretic, promote the production of body fluid, quench the thirst, also can reduce blood glucose.
Radix achyranthis asperae (Herba Achyranthis Asperae), toil, cold; Return liver, lung, urinary bladder channel; Can diuretic, invigorate blood circulation, nourishing stomach-YIN, born fluid, except deficiency-heat.
Radix Puerariae, Radix achyranthis asperae (Herba Achyranthis Asperae) both for making, auxiliary said medicine plays a role.
Determine that Effective Component of Chinese Medicine extracting method of the present invention comprises the steps:
(1) determine and extract route
The prepared slices of Chinese crude drugs that take by following weight proportion: 20 parts of the Radixs Astragali, 10 parts of Fructus Rosae Laevigatae, 10 parts of Rhizoma Chuanxiongs, 15 parts of Flos abelmoschi manihots, 5 parts of Radix Et Rhizoma Rhei, 15 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 10 parts of Radix Puerariaes.
1. draft extraction route
Draft three and extract route: (a) Radix Astragali, Fructus Rosae Laevigatae, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Et Rhizoma Rhei, Radix achyranthis asperae (Herba Achyranthis Asperae), Radix Puerariae water extraction, (b) Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae merge with ethanol extraction and Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) water extraction, (c) Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae with ethanol extraction once, medicinal residues and Fructus Rosae Laevigatae, Radix Et Rhizoma Rhei, Radix achyranthis asperae (Herba Achyranthis Asperae) share water extraction and merge.
2. extract the Pharmacodynamics screening of route
(1) model is set up and grouping
Screen with pharmacodynamic experiment and assay, adopt type 2 diabetes mellitus KK-Ay mice (Beijing HFK Bio-Technology Co., Ltd. in 8 week age, production permit number: SCXK capital 2009-0004) set up diabetic nephropathy model, setting up C57BL/6J mice (Beijing HFK Bio-Technology Co., Ltd., production permit number: SCXK capital 2009-0007) is blank group (CTL).Blank group C57BL/6J mice in 8 week age gives normal diet and feeds, and KK-Ay mice gives high lipid food and feeds.KK-Ay mice random blood sugar >=16.7mmol/L after 4 weeks, thinks that modeling is successful.The successful KK-Ay mice of modeling is divided into model group (M), a group, b group, c group at random.
(2) administration
Modeling success starts administration next day, a group, b group, c group is given respectively to the extract 20g(crude drug of above-mentioned circuit a group, b group, c group) kg
-1d
-1gavage, model group is given equal-volume distilled water, successive administration 12 weeks.
(3) index detects
Experimental session, in the time of 12 week age, 16 week age, 20 weeks, 24 week age, is placed in metabolic cage by each group of mice and collects 24h urine and record urine amount, adopts euzymelinked immunosorbent assay (ELISA) to measure 24h microdose urine protein content, monthly measures once.Result (in table 1) shows: after three kinds of extract administrations, mouse retention albumin excretion rate and model group more all reduce and have a significant difference, but b class mean is minimum, the general state of mice more approaches blank group as the mental status, hair color, and paste-forming rate is minimum in three simultaneously.
Table 1 different time points respectively organize the comparison of mouse retention albumin excretion rate (
) n=7-8
Note: △ shows and the comparison of blank group, P<0.05; ▲ show and model group comparison, P<0.05
Shown by the above results, preferred route is: the Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot ethanol extraction and Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) water extraction merge, and therefore carry out the optimization of extraction process condition for it.
(2) optimization of extraction process condition
1. ethanol extraction extracting section process optimization
Adopt punctate opacity of the cornea design experiment to determine the extracting method of alcohol reflux according to the character of each medicine main component in prescription, and carry out trial test, determine that influence factor is mainly amount of alcohol, concentration of alcohol, extraction time and extraction time, and its scope is investigated.Wherein extraction time is discontinuous variable, is unsuitable for regression treatment, therefore in advance extraction time is carried out to single factor investigation, finds taking 2 times as good.
Take the Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot four Chinese medicine by above-mentioned recipe quantity, design three factor table arrangement tests according to punctate opacity of the cornea.Carry out the reflux, extract, of the different amount of alcohol of variable concentrations, filter, collect filtrate, reclaim ethanol, be concentrated into thick extractum, dry, pulverize, be prepared into dried cream powder.
(1) determine evaluation index and detection method
1) overall desirability of choosing astragaloside, puerarin, Determination of Hyperoside (mg/g crude drug amount) is evaluation index.
2) determine astragaloside assay method in Chinese medicine compound
The sample solution that astragaloside is measured is prepared as follows: get dried cream powder (being approximately equivalent to crude drug 4g) prepared by above-mentioned a certain amount of said method, accurately weighed, the 20mL slight fever that adds water makes to dissolve, with water saturated n-butanol extraction 4 times, each 60mL, merge n-butanol extracting liquid, with ammonia solution extraction 2 times, each 60mL, discard ammonia solution, by n-butyl alcohol liquid evaporate to dryness, residue add water 5mL make dissolve, let cool, by D101 type macroporous resin column (1.5cm × 12cm), with water 50mL eluting, discard water liquid, use 30mL40% ethanol elution, discard eluent, use again 80mL70% ethanol elution, collect eluent evaporate to dryness, dissolve with methanol standardize solution are in 5mL volumetric flask, make sample liquid.
Chromatographic condition is: chromatographic column: C
18(250mm × 4.6mm, 5 μ are m); Mobile phase: acetonitrile-water (32: 68); Volume flow: 1.0mL/min; Drift tube temperature: 101 DEG C; Flow rate of carrier gas: 2.7L/mL.
Take its excess-three kind prepared slices of Chinese crude drugs except the Radix Astragali in prescription ratio, prepare negative sample solution; Take astragaloside reference substance, add dissolve with methanol standardize solution, be mixed with the reference substance solution containing astragaloside 0.528mg/mL; Get the each 20 μ L of reference substance solution, sample solution and negative control solution and inject high performance liquid chromatograph, result shows: need testing solution goes out peak in the time identical with astragaloside reference substance, negative control is noiseless, the results are shown in Figure 1, A astragaloside reference substance in figure, B sample, C negative sample.
3) determine the assay method of puerarin in Chinese medicine compound, hyperin
The sample solution that puerarin, hyperin are measured is prepared as follows: get above-mentioned dried cream powder 0.1g, accurately weighed, be placed in 100mL tool plug conical flask, precision adds methanol 25mL, weighed weight, supersound extraction 30min, let cool rear weighed weight, supply the weight of less loss with methanol, filter, get subsequent filtrate as sample solution.
Chromatographic condition is: chromatographic column:
sTAR LP RP-18endcapped(5 μ m), mobile phase: acetonitrile-0.1% phosphoric acid (15:85), flow velocity: 1mL/min, detect wavelength: 250nm(puerarin) and 360nm(hyperin).
Precision takes puerarin, hyperin reference substance respectively, adds dissolve with methanol standardize solution, is mixed with the reference substance solution containing puerarin 401.2 μ g/mL, hyperin 560.6 μ g/mL; Take its excess-three taste decoction pieces except Radix Puerariae by prescription, prepare negative sample solution; Getting the each 10 μ L of reference substance solution, sample solution and negative control solution is injected in high performance liquid chromatograph, result shows that sample solution goes out peak in the time identical with puerarin reference substance, negative control is noiseless, the results are shown in Figure 2, in figure, A is puerarin reference substance, B is sample, the negative sample of C.
Take its excess-three kind prepared slices of Chinese crude drugs except Flos abelmoschi manihot by prescription, prepare negative sample solution, getting the each 10 μ L of reference substance solution, sample solution and negative sample solution is injected in high performance liquid chromatograph, result shows: it is noiseless that sample solution goes out peak negative control in the time identical with hyperin reference substance, the results are shown in Figure A in 3, figure is that hyperin reference substance B is the negative sample of sample C.
(2) determine ethanol extraction part Optimizing Technical
Astragaloside, puerarin, three indexs of Determination of Hyperoside are normalized to i.e. d by Hansan method
ij=(X
ij-X
min)/(X
max-X
min), ask calculation geometric mean with " the normalizing value " of each index, obtain overall desirability, its formula is as follows: OD=(d
1× d
2× d
n)
1/k(wherein n is index number).
Data in application Design-Expert8.0 software his-and-hers watches 2 are processed, and carry out Multiple Non Linear Regression matching, result, software recommend adoption quadratic polynomial model taking OD value as index.Obtain concentration of alcohol (X
1), solid-liquid ratio (X
2) and extraction time (X
3) and OD value between the multinomial regression equation of secondary, model terms P < 0.05, illustrates Y and X
1, X
2, X
3the relation of regression equation is significant, loses and intends a P > 0.05, illustrates that in gained equation and actual matching, Non-normal errors proportion is little, the Representation Equation Y and X
1, X
2, X
3the relation of regression equation is good.Regression equation is (taking code as independent variable): Y=0.59-0.042X
1+ 0.067X
2+ 0.073X
3+ 0.078X
1x
2-0.071X
1x
3+ 0.11X
2x
3-0.13X
1 2-0.047X
2 2-0.011X
3 2, regression equation coefficient r
2=0.8083(r=0.8991).According to regression equation, be 0 o'clock keeping 1 factor encoded radio, by the three-dimensional response surface figure of other 2 factors of Design-Expert8.0 Software on Drawing and response value relation, the results are shown in Figure 4 is ethanol extraction part punctate opacity of the cornea design response surface 3D figure.
By Design-Expert8.0 software analysis, the optimum process that obtains maximum and be ethanol extraction part is concentration of alcohol (X
1) be 63%, solid-liquid ratio (X
2) be 12, extraction time (X
3) be 150min, now the maximum estimated value of OD value is 0.772.
Table 2 punctate opacity of the cornea contrived experiment arranges and result
2. the optimization of water extraction extracting section technique
Adopt positive quadraturing design test on list of references and trial test basis, determined influence factor and horizontal extent, adopt L
9(3
4) orthogonal water-glass tests, and taking the rate of transform of extracting rear effective ingredient chrysophanic acid as index, investigates the multiple (A) that adds water, extraction time (B), 3 kinds of factors of extraction time (C) to extracting the impact of result.
(1) determine evaluation index and detection method
1) taking the rate of transform of extracting rear effective ingredient chrysophanic acid as index.
2) assay method of chrysophanic acid in Chinese medicine compound
The sample preparation that chrysophanic acid is measured is as follows: precision takes the above-mentioned dried cream powder that is approximately equivalent to 0.15g Radix Et Rhizoma Rhei crude drug amount and is placed in tool plug conical flask, presses routine step prepare sample with reference to 2010 editions " Chinese Pharmacopoeia " Radix Et Rhizoma Rhei assay items.
Precision takes chrysophanic acid reference substance, adds dissolve with methanol standardize solution, is mixed with the reference substance solution containing chrysophanic acid 18.84 μ g/mL.
First adopt pharmacopeia condition determination mobile phase: methanol: 0.1% phosphoric acid (volume ratio 85:15); Detecting wavelength is the assay to the chrysophanic acid in compound extract under 254nm, and as seen from Figure 5 because appearance time is very fast, each component separating degree is not good, and impurity peaks is more, unsuitable identification, and conditions of streaking is comparatively serious.
Employing chromatographic condition is, chromatographic column: Kromasil100-5C
18(250mm × 4.6mm, 5 μ are m); Mobile phase: methanol: 0.1% phosphoric acid (volume ratio 75:25); Flow velocity: 1.0mL/min; Detection wavelength is 436nm; Column temperature: room temperature; Sample size: 10 μ l.
The results are shown in Figure 6, wherein A is reference substance, and B is sample, the negative contrast of C.1 aloe-emodin, 2 chrysophanic acids, 3 emodins, 4 chrysophanols, 5 physciones.
The a certain amount of above-mentioned chrysophanic acid reference substance solution of accurate absorption respectively, dilution obtains the solution of 0.60 μ g/mL, 0.94 μ g/mL, 1.88 μ g/mL, 2.36 μ g/mL, 9.42 μ g/mL, 18.84 μ g/mL6 kind variable concentrations, sample introduction 10 μ l respectively, draw chrysophanic acid standard curve, calculated the content of chrysophanic acid by standard curve, calculate the rate of transform.
(2) determine water extraction part Optimizing Technical
Take Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) with original prescription ratio, the decoction pieces 1h that is first soaked in water, by L
9(3
4) orthogonal table testing program extracts, and regulates electric jacket temperature controller at nearly 100 DEG C of gears, carries out constant temperature backflow.8 layers of filtered through gauze for medicinal liquid, 70 DEG C of concentrating under reduced pressure, 60 DEG C of vacuum dryings.No. four sieves of the broken mistake of dried cream powder save backup to exsiccator.Orthogonal test repeats 3 times.
Table 3 orthogonal test factor level table
Table 4 orthogonal test L
9(3
4) arrange and investigate result (
)
Table 5 chrysophanic acid rate of transform influence factor the results of analysis of variance
Result draws by orthogonal and variance analysis method, and the optimum extraction process of water extraction part is: with the water extraction of 10 times of amounts 3 times, extract 1.5h at every turn.Verify three times, result Radix Et Rhizoma Rhei rate of transform meansigma methods is 84.96%.
3. determine the preparation method for the treatment of diabetic nephropathy Effective Component of Chinese Medicine
Take by weight ratio Chinese crude drug, the Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot are added by weight to 50～80% alcohol reflux 1～4 time of 8～14 times, each 0.5～3 hour, collecting extracting solution and being concentrated into relative density was 1.08～1.15 clear paste; Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 1～4 time of 8～12 times, each 0.5～2 hour, collecting extracting solution and being concentrated into relative density was 1.08～1.15 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.30～1.40 thick paste.
(3) optimization of granulating process condition
Take the prepared slices of Chinese crude drugs by the weight proportion of 20 parts of the Radixs Astragali, 10 parts of Fructus Rosae Laevigatae, 10 parts of Rhizoma Chuanxiongs, 15 parts of Flos abelmoschi manihots, 5 parts of Radix Et Rhizoma Rhei, 15 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 10 parts of Radix Puerariaes, the Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae are added by weight to 63% alcohol reflux 2 times of 12 times, each 2.5 hours, collecting extracting solution and being concentrated into relative density was 1.1 clear paste; Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 3 times of 10 times, each 1.5 hours, collecting extracting solution and being concentrated into relative density was 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste, add adjuvant to make the dosage form that is applicable to clinical use.
Be prepared into granule concrete grammar as follows:
1 part of 1 part of said extracted thing and 1 part of soluble starch, 1 part, dextrin, 1 part of microcrystalline Cellulose, 1 part of lactose, 1 part, mannitol, xylitol are mixed homogeneously respectively mixing, add appropriate ethanol soft material processed, cross 14 order granulations, in baking oven, 65 DEG C are dried 30min, cross 10 mesh sieve granulate.Measure respectively its hydroscopicity, result as shown in Figure 7, show, in 6 groups of samples, hydroscopicity order is lactose < mannitol < microcrystalline Cellulose < dextrin < soluble starch < xylitol, comprehensive its granulation is difficult to degree, outward appearance, melting, under consideration, select soluble starch, dextrin lactose, microcrystalline Cellulose, the mannitol weak with hygroscopicity to mix use, in maintaining the form of granule, reduce the hygroscopicity of granule.
1 part of said extracted thing and dextrin and microcrystalline Cellulose are respectively (1:1, 2:1, 1 part, mixture 3:1), 1 part of said extracted thing and dextrin and lactose are respectively (1:1, 2:1, 1 part, mixture 3:1), 1 part of said extracted thing and dextrin and mannitol are respectively (1:1, 2:1, 1 part, mixture 3:1), 1 part of said extracted thing and soluble starch and mannitol are respectively (1:1, 2:1, 1 part, mixture 3:1), totally 12 duplicate samples, mix homogeneously mixes respectively, add appropriate ethanol soft material processed, cross 14 order granulations, in baking oven, 65 DEG C are dried 30min, cross 10 mesh sieve granulate.Measure respectively its hydroscopicity, result shows, after dextrin mixes in varing proportions with different auxiliary material, then mixes with 1 ︰ 1 with dried cream powder, and 12 duplicate samples hydroscopicities all decline to some extent.More (as shown in Figure 8) that wherein dextrin and mannitol 1 ︰ 1 reduce, after moisture absorption, grain shape maintains better, and granulate be easier to, dissolubility is better, be suitable for simultaneously diabetics use.Consider lower selection dextrin and mannitol 1 ︰ 1 writes out a prescription.
Therefore preferred pelletization is: 1 part-5 parts of said extracted thing 400-600 part, dextrin 200-300 part, mannitol 200-300 part and aspartames.More preferably: above-mentioned 2.5 parts of 500 parts of thing extracts, 250 parts, dextrin, 250 parts, mannitol and the aspartames of getting, after granulating, granule sucting wet curve as shown in Figure 9.
Be below specific embodiments of the invention, technical scheme of the present invention is done to further description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
(1) take Chinese crude drug by following weight proportion, 20 parts of the Radixs Astragali, 10 parts of Fructus Rosae Laevigatae, 10 parts of Rhizoma Chuanxiongs, 15 parts of Flos abelmoschi manihots, 5 parts of Radix Et Rhizoma Rhei, 15 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 10 parts of Radix Puerariaes.
(2) Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot are added by weight to 63% alcohol reflux 2 times of 12 times, each 150 minutes, collect extracting solution.
The content of high effective liquid chromatography for measuring astragaloside, hyperin, puerarin.Measure the content of astragaloside, detector: evaporative light scattering detector; The chromatographic column of high performance liquid chromatography is C
18; Mobile phase: acetonitrile-water (32: 68); Flow rate of mobile phase: 1.0mL/min; Drift tube temperature: 101 DEG C; Flow rate of carrier gas: 2.7L/mL; Sample size is 20 μ l.Measure the content of hyperin, puerarin, detector: UV-detector; Mobile phase: acetonitrile-0.1% phosphate aqueous solution (volumn concentration) (15:85); The chromatographic column of high performance liquid chromatography: C
18; Flow rate of mobile phase: 1.0mL/min; Detect wavelength: puerarin is 250nm, and hyperin is 360nm, and sample size is 10 μ l.
The content of astragaloside is that the content of 1.2954mg/g, puerarin is that the content of 47.8261mg/g, hyperin is 7.7515mg/g as calculated.
It is 1.1 clear paste that said extracted liquid is concentrated into relative density.
(3) Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 3 times of 10 times, each 1.5 hours, collect extracting solution.
High effective liquid chromatography for measuring Rhein Content, mobile phase is the mixed liquor of methanol and the volumn concentration phosphate aqueous solution composition that is 0.1%, the volume ratio of methanol and phosphate aqueous solution is 75:25; The chromatographic column of high performance liquid chromatography is C
18, flow rate of mobile phase is 1.0mL/min; Detection wavelength is 436nm, and sample size is 10 μ l.
As calculated, the content of chrysophanic acid is 3.5598mg/g.
It is 1.1 clear paste that said extracted liquid is concentrated into relative density.
(4) above step 2, two kinds of clear paste of 3 are merged, continue to be concentrated into relative density and be 1.4 thick paste, add following adjuvant granulation preparation.Consisting of of this granular preparation: 2.5 parts of 500 parts of above-mentioned thick pastes, 250 parts, dextrin, 250 parts, mannitol and aspartames.
Take Chinese crude drug by following weight proportion, 15 parts of the Radixs Astragali, 13 parts of Fructus Rosae Laevigatae, 7 parts of Rhizoma Chuanxiongs, 15 parts of Flos abelmoschi manihots, 3 parts of Radix Et Rhizoma Rhei, 15 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 13 parts of Radix Puerariaes.
The Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot are added by weight to 50% alcohol reflux 1 time of 14 times, each 180 minutes, collect extracting solution, the content that adopts the method identical with embodiment 1 to measure astragaloside is that the content of 1.3074mg/g, puerarin is that the content of 39.6242mg/g, hyperin is 7.1072mg/g, is concentrated into relative density and is 1.1 clear paste; Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 2 times of 10 times, each 1 hour, collect extracting solution, adopting the content of the method mensuration chrysophanic acid identical with embodiment 1 is 2.7084mg/g, is concentrated into relative density and is 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste, add following adjuvant granulation preparation.Consisting of of this granular preparation: 2.5 parts of 500 parts of above-mentioned thick pastes, 250 parts, dextrin, 250 parts, mannitol and aspartames.
Take Chinese crude drug by following weight proportion, 20 parts of the Radixs Astragali, 7 parts of Fructus Rosae Laevigatae, 10 parts of Rhizoma Chuanxiongs, 10 parts of Flos abelmoschi manihots, 3 parts of Radix Et Rhizoma Rhei, 10 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 7 parts of Radix Puerariaes.
The Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot are added by weight to 70% alcohol reflux 4 times of 8 times, each 30 minutes, collect extracting solution, the content that adopts the method identical with embodiment 1 to measure astragaloside is that the content of 1.2099mg/g, puerarin is that the content of 40.2475mg/g, hyperin is 7.0431mg/g, is concentrated into relative density and is 1.1 clear paste; Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 4 times of 8 times, each 0.5 hour, collect extracting solution, adopting the content of the method mensuration chrysophanic acid identical with embodiment 1 is 2.5489mg/g, is concentrated into relative density and is 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste, add following adjuvant granulation preparation.Consisting of of this granular preparation: 2.5 parts of 500 parts of above-mentioned thick pastes, 250 parts, dextrin, 250 parts, mannitol and aspartames.
Take Chinese crude drug by following weight proportion, 25 parts of the Radixs Astragali, 7 parts of Fructus Rosae Laevigatae, 7 parts of Rhizoma Chuanxiongs, 20 parts of Flos abelmoschi manihots, 6 parts of Radix Et Rhizoma Rhei, 10 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 12 parts of Radix Puerariaes.
The Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot are added by weight to 65% alcohol reflux 3 times of 10 times, each 120 minutes, collect extracting solution, the content that adopts the method identical with embodiment 1 to measure astragaloside is that the content of 1.2017mg/g, puerarin is that the content of 41.0188mg/g, hyperin is 7.9355mg/g, is concentrated into relative density and is 1.1 clear paste; Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 4 times of 8 times, each 1.5 hours, collect extracting solution, adopting the content of the method mensuration chrysophanic acid identical with embodiment 1 is 3.3461mg/g, is concentrated into relative density and is 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste, add following adjuvant granulation preparation.Consisting of of this granular preparation: 2.5 parts of 500 parts of above-mentioned thick pastes, 350 parts, dextrin, 350 parts, mannitol and aspartames.
Take Chinese crude drug by following weight proportion, 20 parts of the Radixs Astragali, 13 parts of Fructus Rosae Laevigatae, 13 parts of Rhizoma Chuanxiongs, 18 parts of Flos abelmoschi manihots, 5 parts of Radix Et Rhizoma Rhei, 10 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 10 parts of Radix Puerariaes.
The Radix Astragali, Radix Puerariae, Rhizoma Chuanxiong, Flos abelmoschi manihot are added by weight to 80% alcohol reflux 2 times of 8 times, each 90 minutes, collect extracting solution, the content that adopts the method identical with embodiment 1 to measure astragaloside is that the content of 1.1564mg/g, puerarin is that the content of 37.2573mg/g, hyperin is 8.6575mg/g, is concentrated into relative density and is 1.1 clear paste; Radix Et Rhizoma Rhei, Fructus Rosae Laevigatae, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 2 times of 12 times, each 2 hours, collect extracting solution, adopting the content of the method mensuration chrysophanic acid identical with embodiment 1 is 3.0489mg/g, is concentrated into relative density and is 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste, add following adjuvant granulation preparation.Consisting of of this granular preparation: 3 parts of 500 parts of above-mentioned thick pastes, 300 parts, dextrin, 300 parts, mannitol and aspartames.
(1) model is set up and grouping
Screen with pharmacodynamic experiment and assay, adopt type 2 diabetes mellitus KK-Ay mice (Beijing HFK Bio-Technology Co., Ltd. in 8 week age, production permit number: SCXK capital 2009-0004) set up diabetic nephropathy model, setting up C57BL/6J mice (Beijing HFK Bio-Technology Co., Ltd., production permit number: SCXK capital 2009-0007) is blank group (CTL).Blank group C57BL/6J mice in 8 week age gives normal diet and feeds, and KK-Ay mice gives high lipid food and feeds.KK-Ay mice random blood sugar >=16.7mmol/L after 4 weeks, thinks that modeling is successful.The successful KK-Ay mice of modeling is divided into model group (M), 1 group, 2 groups, 3 groups, 4 groups, 5 groups at random.
(2) administration
Modeling success starts administration next day, to 1 group, 2 groups, 3 groups, 4 groups, the 5 groups extract thick paste 20gkg that give respectively above-described embodiment 1,2,3,4,5
-1d
-1gavage, model group is given equal-volume distilled water, successive administration 12 weeks.
(3) index detects
Experimental session, in the time of 12 week age, 16 week age, 20 weeks, 24 week age, is placed in metabolic cage by each group of mice and collects 24h urine and record urine amount, adopts euzymelinked immunosorbent assay (ELISA) to measure 24h microdose urine protein content, monthly measures once.Result (in table 6) shows: after five kinds of extract administrations, mouse retention albumin excretion rate and model group more all reduce and have a significant difference.
Table 6 different time points respectively organize the comparison of mouse retention albumin excretion rate (
) n=7-8
Note: △ shows and the comparison of blank group, P<0.05; ▲ show and model group comparison, P<0.05.
Claims (10)
1. treat the Chinese medicine composition of diabetic nephropathy for one kind, it is characterized in that, this Chinese medicine composition is to be made up of the prepared slices of Chinese crude drugs of following weight proportioning: 15～25 parts of the Radixs Astragali, 7～13 parts of Fructus Rosae Laevigatae, 7～13 parts of Rhizoma Chuanxiongs, 10～20 parts of Flos abelmoschi manihots, 2～8 parts of Radix Et Rhizoma Rhei, 7～13 parts of Radix Puerariaes, 10～20 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s.
2. the Chinese medicine composition for the treatment of diabetic nephropathy as claimed in claim 1, it is characterized in that, described Chinese medicine composition is to be made up of the prepared slices of Chinese crude drugs of following weight proportioning: 20 parts of the Radixs Astragali, 10 parts of Fructus Rosae Laevigatae, 10 parts of Rhizoma Chuanxiongs, 15 parts of Flos abelmoschi manihots, 5 parts of Radix Et Rhizoma Rhei, 15 parts of Radix achyranthis asperae (Herba Achyranthis Asperae)s, 10 parts of Radix Puerariaes.
3. treat the preparation method of diabetic nephropathy Chinese medicine composition for one kind, it is characterized in that, described method comprises: take the prepared slices of Chinese crude drugs by weight proportion as claimed in claim 1 or 2, the Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae are added by weight to 50～80 volume % alcohol reflux 1～4 time of 8～14 times, each 0.5～3 hour, collecting extracting solution and being concentrated into relative density was 1.08～1.15 clear paste; Fructus Rosae Laevigatae, Radix Et Rhizoma Rhei, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 1～4 time of 8～12 times, each 0.5～2 hour, collecting extracting solution and being concentrated into relative density was 1.08～1.15 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.30～1.40 thick paste.
4. preparation method as claimed in claim 3, it is characterized in that, described method comprises: take the prepared slices of Chinese crude drugs by weight proportion as claimed in claim 1 or 2, the Radix Astragali, Rhizoma Chuanxiong, Flos abelmoschi manihot, Radix Puerariae are added by weight to 63% alcohol reflux 2 times of 12 times, each 2.5 hours, collecting extracting solution and being concentrated into relative density was 1.1 clear paste; Fructus Rosae Laevigatae, Radix Et Rhizoma Rhei, Radix achyranthis asperae (Herba Achyranthis Asperae) are added by weight to the water reflux, extract, 3 times of 10 times, each 1.5 hours, collecting extracting solution and being concentrated into relative density was 1.1 clear paste; Above two kinds of clear paste are merged, continue to be concentrated into relative density and be 1.4 thick paste.
5. a Chinese medicine composition for the treatment of diabetic nephropathy, is characterized in that, it is prepared according to method described in claim 3 or 4.
6. an oral preparation of Chinese traditional medicinal for the treatment of diabetic nephropathy, is characterized in that, it is made up of with adjuvant Chinese medicine composition claimed in claim 5 and medicinal or food.
7. treatment diabetic nephropathy oral preparation of Chinese traditional medicinal as claimed in claim 6, it is characterized in that, described oral formulations is granule, and this granule is made up of the material of following weight proportioning: 1～5 part of 400～600 parts of described thick pastes, 200～300 parts, dextrin, 200～300 parts, mannitol and aspartame.
8. the detection method of Chinese medicine composition described in claim 5, it is characterized in that, ethanol extraction part is taking the content of astragaloside, hyperin, puerarin as testing index, minima is respectively 0.8214mg/g, 26.3417mg/g, 5.4362mg/g, water extraction part is taking the content of chrysophanic acid as testing index, and minima is 2.5141mg/g.
9. measure described in claim 5 method of hyperin and puerarin content in Chinese medicine composition for one kind simultaneously, it is characterized in that, the method is that detected sample is measured by high performance liquid chromatography, the mobile phase of this high performance liquid chromatography is acetonitrile and 0.05%～0.3%(volumn concentration) the mixed liquor of phosphate aqueous solution composition, the volume ratio of acetonitrile and phosphate aqueous solution is (10～30): (90～70); The chromatographic column that high performance liquid chromatography adopts is C
18, the flow velocity of mobile phase is (0.8～1.2) mL/min; Detect wavelength: puerarin is 250nm, and hyperin is 360nm, and sample size is 8～12 μ l.
10. measure described in claim 5 method of Rhein Content in Chinese medicine composition for one kind, it is characterized in that, the method is that detected sample is measured by high performance liquid chromatography, the mobile phase of high performance liquid chromatography is the mixed liquor of methanol and the volumn concentration phosphate aqueous solution composition that is 0.05～0.3%, and the percent by volume of methanol in mixed liquor is 60～85%; The chromatographic column of high performance liquid chromatography is C
18, flow rate of mobile phase is 0.8～1.2mL/min; Detection wavelength is 436nm, and sample size is 8～12 μ l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410133378.9A CN103830380B (en) | 2014-03-19 | 2014-04-03 | A kind of Chinese medicine composition for the treatment of diabetic nephropathy and preparation method thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410103358.7 | 2014-03-19 | ||
| CN201410103358 | 2014-03-19 | ||
| CN201410133378.9A CN103830380B (en) | 2014-03-19 | 2014-04-03 | A kind of Chinese medicine composition for the treatment of diabetic nephropathy and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103830380A true CN103830380A (en) | 2014-06-04 |
| CN103830380B CN103830380B (en) | 2015-11-25 |
Family
ID=50794483
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410133378.9A Active CN103830380B (en) | 2014-03-19 | 2014-04-03 | A kind of Chinese medicine composition for the treatment of diabetic nephropathy and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103830380B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104107239A (en) * | 2014-05-29 | 2014-10-22 | 陈凯 | Traditional Chinese medicine composition for treating hypertension and preparation method thereof |
| CN105288410A (en) * | 2015-11-28 | 2016-02-03 | 青岛麦瑞特医药技术有限公司 | Traditional Chinese medicine composition for treating diabetic nephropathy and application of traditional Chinese medicine composition |
| CN112656920A (en) * | 2021-01-29 | 2021-04-16 | 成都中医药大学附属医院 | Medicine composition for treating diabetic nephropathy and renal failure and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101313942A (en) * | 2007-05-30 | 2008-12-03 | 江苏正大天晴药业股份有限公司 | Chinese medicine composition for treating nephropathy |
| CN102228536A (en) * | 2011-06-09 | 2011-11-02 | 首都医科大学 | Chinese herbal extract for treating diabetic nephropathy and preparation method thereof |
| CN102895567A (en) * | 2011-07-28 | 2013-01-30 | 浙江天草中药材专业合作社 | Chinese herbal medicine for treating diabetes and complications thereof |
| CN103566350A (en) * | 2013-11-07 | 2014-02-12 | 李涛 | Pharmaceutical composition having blood sugar reducing effect |
-
2014
- 2014-04-03 CN CN201410133378.9A patent/CN103830380B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101313942A (en) * | 2007-05-30 | 2008-12-03 | 江苏正大天晴药业股份有限公司 | Chinese medicine composition for treating nephropathy |
| CN102228536A (en) * | 2011-06-09 | 2011-11-02 | 首都医科大学 | Chinese herbal extract for treating diabetic nephropathy and preparation method thereof |
| CN102895567A (en) * | 2011-07-28 | 2013-01-30 | 浙江天草中药材专业合作社 | Chinese herbal medicine for treating diabetes and complications thereof |
| CN103566350A (en) * | 2013-11-07 | 2014-02-12 | 李涛 | Pharmaceutical composition having blood sugar reducing effect |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104107239A (en) * | 2014-05-29 | 2014-10-22 | 陈凯 | Traditional Chinese medicine composition for treating hypertension and preparation method thereof |
| CN105288410A (en) * | 2015-11-28 | 2016-02-03 | 青岛麦瑞特医药技术有限公司 | Traditional Chinese medicine composition for treating diabetic nephropathy and application of traditional Chinese medicine composition |
| CN112656920A (en) * | 2021-01-29 | 2021-04-16 | 成都中医药大学附属医院 | Medicine composition for treating diabetic nephropathy and renal failure and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103830380B (en) | 2015-11-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105687994B (en) | A kind of Chinese medicine composition and preparation method thereof for treating chronic renal failure | |
| WO2022036779A1 (en) | Huashibaidu granule, preparation method therefor and anti-viral drug | |
| CN104758515A (en) | Traditional Chinese medicinal composition for treating nephropathy as well as preparation method and detection method thereof | |
| CN101002909A (en) | Antiviral traditional Chinese medicine, its preparing method and quality control method | |
| CN103830380B (en) | A kind of Chinese medicine composition for the treatment of diabetic nephropathy and preparation method thereof | |
| CN110780004A (en) | Method for establishing fingerprint of traditional Chinese medicine composition or preparation thereof with depression mood regulating effect | |
| CN101940642B (en) | Chinese medicinal composition and application thereof | |
| CN102068507A (en) | Preparation method of Chinese medicinal capsules for tonifying lung and activating blood circulation | |
| CN102920964B (en) | Traditional Chinese medicine preparation for curing cough | |
| CN106138179A (en) | A kind of purposes of Radix Astragali extract | |
| CN102266503A (en) | Sugar-free anemopyretic cold granules and preparation method thereof | |
| CN102885928B (en) | Chinese medicinal injection for treating jaundice and preparation method thereof | |
| CN116808147A (en) | A Chinese medicinal composition for treating diabetic nephropathy and its quality detection method | |
| CN108714205B (en) | Traditional Chinese medicine granule for treating spleen-kidney yang deficiency type IgA nephropathy and preparation method and application thereof | |
| CN103316074B (en) | Medicine composite of halenia corni extract, astragalus extract and liquorice extract as well as preparation and application of medicine composite | |
| CN113769023B (en) | A Chinese medicinal composition for treating hypertension and its quality detection method | |
| CN102641402B (en) | Preparation method of traditional Chinese medicine compound preparation for treating nephritis | |
| CN102697932B (en) | Medicinal composition for treating skin itch and quality detection method | |
| CN101869675B (en) | Chinese medicinal composition granules for treating summer-heat damp cold and preparation method thereof | |
| CN101062204B (en) | Method for preparing capsule made from asarum, scutellaria and so on and the capsule prepared by using said method | |
| CN101422522B (en) | Preparation method of traditional Chinese medicine preparation | |
| CN114344358A (en) | Eucommia ulmoides composition containing aucubin and geniposide, preparation and application | |
| CN102233084B (en) | Chinese medicinal composition for treating prostatitis and preparation method thereof | |
| CN111068024A (en) | Chinese medicine compound preparation and its preparing method | |
| CN105548426B (en) | A kind of detection method for the Chinese medicine composition for treating chronic renal failure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |