Summary of the invention
The object of the invention is to solve blank of the prior art, a kind of method of utilizing municipal sludge to prepare flowers matrix is provided, not only efficiently solve the problem of municipal sludge, and provide a kind of efficient nutraceutical matrix for the growth of flowers.
The technical solution adopted in the present invention is: a kind of method of utilizing municipal sludge to prepare flowers matrix, comprises the following steps:
Step 1, by wheat stalk and air-dry after municipal sludge pulverize, and the screen filtration that is 1-2mm with aperture respectively, according to the mass ratio mixing of 4:1, makes mixed-matrix I by the wheat stalk after filtering and municipal sludge after stirring, for subsequent use;
Step 2, in mixed-matrix I, add water, the mass percent concentration that makes water in mixed-matrix I is 68-72%, in mixed-matrix I, add microbial starter culture afterwards, and mixed-matrix I is 5:1 with the ratio of microbial starter culture, wherein the unit of mixed-matrix I is m
3, the unit of microbial starter culture is kg, makes mixed-matrix II after stirring, it is the environment of 30 ℃ that mixed-matrix II is put into temperature, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare the required each raw material weight umber of starter and be respectively soybean extraction 20-25 part, subtilis 1-2 part, silicate bacteria 3-4 part, viride 2-4 part, Lactococcus lactis 8-12 part, actinomycetes 2-4 part, colloid bacillus cereus 1-2 part, 2 parts of rice Flavobacteriums, yeast 3-6 part, azotobacter chroococcum 10-13 part, cellulase 8-12 part, proteinase 8-12 part and Starch phosphorylase 5-6 part,
Step 3, by matrix III proceed in proving room, ferment 25 days afterwards taking-up make product.And in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/minm
3, in 16-25 days proving rooms, strength ofdraft is 20L/minm
3;
Described in step 2, the preparation method of microbial starter culture comprises the following steps:
The preparation of step 1, soybean extraction
(1), by soybean clean after dry, pulverize, the screen filtration that is 1mm with diameter afterwards, makes soyflour, for subsequent use;
(2), soyflour is added in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, make mixed solution I after stirring, every gram of phosphoric acid buffer volume corresponding to soyflour is 10mL;
(3) the mixed solution I, step (2) being made is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, in the phosphate buffered saline buffer that afterwards precipitation is dissolved in to pH and is 6.5, volumetric molar concentration is 0.05mol/L, then proceed to centrifugal 15min in the whizzer that rotating speed is 4000r/min, collect supernatant liquor, make soybean extraction, for subsequent use;
Step 2, take each component according to the parts by weight of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, make complex liquid;
Step 4, soybean extraction is put into frequency is that the ultrasonic wave of 20kHz is processed 20min, and every processing 3min stops 15s, to the complex liquid that adds step 3 to make in soybean extraction, stirs afterwards.
Subtilis in the present invention (
bacillus subtilis) culture presevation number is CICC20632; Viride (
trichoderma viride) culture presevation number is CICC40202; Lactococcus lactis (
lactococcus lactis) culture presevation number is CICC23610; Actinomycetes (
dactylosporangium sp.) culture presevation number is ACCC40661; Azotobacter chroococcum (
azotobacter chrooccum) culture presevation number is ACCC10098; Colloid bacillus cereus (
bacillus mucilaginosus) culture presevation number is ACCC02983; Rice Flavobacterium (
flavobacterium oryzae) culture presevation number is CGMCC1.1585; Yeast (
saccharomyces sp.) culture presevation number is CCTCC AY 91003; Above bacterial classification is all purchased from Beijing North Na Chuanlian Bioteknologisk Institut.
beneficial effect
One, the present invention is by taking the starter that is added with soybean extraction to ferment, greatly reduce intensification and the fermentation time of wheat stalk and municipal sludge fermentation, improve fermented substrate to the absorbing of starter, enriched the nutritive ingredient after fermentation, also improved working efficiency simultaneously.
Two, the soybean extraction in the present invention first mixes with complex liquid by ultrasonication again, composition and soybean extraction in complex liquid are merged evenly, improve municipal sludge and the stalk absorption rate to starter, made municipal sludge and stalk fermentation more thorough.In addition, the present invention, in the pause of having a rest with ultrasonic treatment time, has avoided the damage of ultrasonic wave to composition in soybean extraction.
Three, the present invention is 40L/minm by strength ofdraft in 1-10 days proving rooms of fermentation
3, in 16-25 days proving rooms, strength ofdraft is 20L/minm
3, make hot stage of the present invention can reach 60 ℃ and maintain 5 ~ 6 days, can kill the disease and pest in matrix.
Four, preparation technology of the present invention is simple, by being combined with multiple-microorganism, enzyme, can decomposite the multiple nutrients material of municipal sludge, has obtained the effect turning waste into wealth, when being beneficial to urban beautification, for the growth of plant provides nutraceutical matrix.
Embodiment
Utilize municipal sludge to prepare a method for flowers matrix, comprise the following steps:
Step 1, by wheat stalk and air-dry after municipal sludge pulverize, and the screen filtration that is 1-2mm with aperture respectively, according to the mass ratio mixing of 4:1, makes mixed-matrix I by the wheat stalk after filtering and municipal sludge after stirring, for subsequent use;
Step 2, in mixed-matrix I, add water, the mass percent concentration that makes water in mixed-matrix I is 68-72%, in mixed-matrix I, add microbial starter culture afterwards, and mixed-matrix I is 5:1 with the ratio of microbial starter culture, wherein the unit of mixed-matrix I is m
3, the unit of microbial starter culture is kg, makes mixed-matrix II after stirring, it is the environment of 30 ℃ that mixed-matrix II is put into temperature, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare the required each raw material weight umber of starter and be respectively soybean extraction 20-25 part, subtilis 1-2 part, silicate bacteria 3-4 part, viride 2-4 part, Lactococcus lactis 8-12 part, actinomycetes 2-4 part, colloid bacillus cereus 1-2 part, 2 parts of rice Flavobacteriums, yeast 3-6 part, azotobacter chroococcum 10-13 part, cellulase 8-12 part, proteinase 8-12 part and Starch phosphorylase 5-6 part,
Step 3, by matrix III proceed in proving room, ferment 25 days afterwards taking-up make product.And in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/minm
3, in 16-25 days proving rooms, strength ofdraft is 20L/minm
3;
Described in step 2, the preparation method of microbial starter culture comprises the following steps:
The preparation of step 1, soybean extraction
(1), by soybean clean after dry, pulverize, the screen filtration that is 1mm with diameter afterwards, makes soyflour, for subsequent use;
(2), soyflour is added in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, make mixed solution I after stirring, every gram of phosphoric acid buffer volume corresponding to soyflour is 10mL;
(3) the mixed solution I, step (2) being made is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, in the phosphate buffered saline buffer that afterwards precipitation is dissolved in to pH and is 6.5, volumetric molar concentration is 0.05mol/L, then proceed to centrifugal 15min in the whizzer that rotating speed is 4000r/min, collect supernatant liquor, make soybean extraction, for subsequent use;
Step 2, take each component according to the parts by weight of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, make complex liquid;
Step 4, soybean extraction is put into frequency is that the ultrasonic wave of 20kHz is processed 20min, and every processing 3min stops 15s, to the complex liquid that adds step 3 to make in soybean extraction, stirs afterwards.
Embodiment 1
Utilize municipal sludge to prepare a method for flowers matrix, comprise the following steps:
Step 1, by wheat stalk and air-dry after municipal sludge pulverize, and the screen filtration that is 1mm with aperture respectively, according to the mass ratio mixing of 4:1, makes mixed-matrix I by the wheat stalk after filtering and municipal sludge after stirring, for subsequent use;
Step 2, in mixed-matrix I, add water, the mass percent concentration that makes water in mixed-matrix I is 68-72%, in mixed-matrix I, add microbial starter culture afterwards, and mixed-matrix I is 5:1 with the ratio of microbial starter culture, wherein the unit of mixed-matrix I is m
3, the unit of microbial starter culture is kg, makes mixed-matrix II after stirring, it is the environment of 30 ℃ that mixed-matrix II is put into temperature, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare the required each raw material weight umber of starter and be respectively 20 parts of soybean extraction, 1 part of subtilis, 3 parts of silicate bacterias, 2 parts of virides, 8 parts of Lactococcus lactis, 2 parts, actinomycetes, 1 part of colloid bacillus cereus, 2 parts of rice Flavobacteriums, 3 parts, yeast, 10 parts of azotobacter chroococcums, 8 parts of cellulases, 5 parts of proteinase 8 part and Starch phosphorylases,
Step 3, by matrix III proceed in proving room, ferment 25 days afterwards taking-up make product.And in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/minm
3, in 16-25 days proving rooms, strength ofdraft is 20L/minm
3;
Described in step 2, the preparation method of microbial starter culture comprises the following steps:
The preparation of step 1, soybean extraction
(1), by soybean clean after dry, pulverize, the screen filtration that is 1mm with diameter afterwards, makes soyflour, for subsequent use;
(2), soyflour is added in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, make mixed solution I after stirring, every gram of phosphoric acid buffer volume corresponding to soyflour is 10mL;
(3) the mixed solution I, step (2) being made is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, in the phosphate buffered saline buffer that afterwards precipitation is dissolved in to pH and is 6.5, volumetric molar concentration is 0.05mol/L, then proceed to centrifugal 15min in the whizzer that rotating speed is 4000r/min, collect supernatant liquor, make soybean extraction, for subsequent use;
Step 2, take each component according to the parts by weight of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, make complex liquid;
Step 4, soybean extraction is put into frequency is that the ultrasonic wave of 20kHz is processed 20min, and every processing 3min stops 15s, to the complex liquid that adds step 3 to make in soybean extraction, stirs afterwards.
Result test: the matrix color after utilizing aforesaid method to become thoroughly decomposed is chocolate, moisture content 49%, the C/N ratio of matrix is reduced to 12.5.60 ℃ of hot stages can maintain 5 days, and T=(C/N is eventually)/(C/N beginning) T<0.6.Available N-P potassium content is respectively 0.67mg/kg, 837.85mg/kg, 3590.57mg/kg.This available nutrient content can meet the needs of plant-growth completely.
Embodiment 2
Utilize municipal sludge to prepare a method for flowers matrix, comprise the following steps:
Step 1, by wheat stalk and air-dry after municipal sludge pulverize, and the screen filtration that is 2mm with aperture respectively, according to the mass ratio mixing of 4:1, makes mixed-matrix I by the wheat stalk after filtering and municipal sludge after stirring, for subsequent use;
Step 2, in mixed-matrix I, add water, the mass percent concentration that makes water in mixed-matrix I is 68-72%, in mixed-matrix I, add microbial starter culture afterwards, and mixed-matrix I is 5:1 with the ratio of microbial starter culture, wherein the unit of mixed-matrix I is m
3, the unit of microbial starter culture is kg, makes mixed-matrix II after stirring, it is the environment of 30 ℃ that mixed-matrix II is put into temperature, for subsequent use;
Described microbial starter culture is by soybean extraction, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum, cellulase, proteolytic enzyme and Starch phosphorylase are made, and prepare the required each raw material weight umber of starter and be respectively 25 parts of soybean extraction, 2 parts of subtilises, 4 parts of silicate bacterias, 4 parts of virides, 12 parts of Lactococcus lactis, 4 parts, actinomycetes, 2 parts of colloid bacillus cereus, 2 parts of rice Flavobacteriums, 6 parts, yeast, 13 parts of azotobacter chroococcums, 12 parts of cellulases, 6 parts of 12 parts, proteolytic enzyme and Starch phosphorylases,
Step 3, by matrix III proceed in proving room, ferment 25 days afterwards taking-up make product.And in 1-10 days proving rooms of fermentation, strength ofdraft is 40L/minm
3, in 16-25 days proving rooms, strength ofdraft is 20L/minm
3;
Described in step 2, the preparation method of microbial starter culture comprises the following steps:
The preparation of step 1, soybean extraction
(1), by soybean clean after dry, pulverize, the screen filtration that is 1mm with diameter afterwards, makes soyflour, for subsequent use;
(2), soyflour is added in the phosphate buffered saline buffer that pH is 5.2, volumetric molar concentration is 0.05mol/L, make mixed solution I after stirring, every gram of phosphoric acid buffer volume corresponding to soyflour is 10mL;
(3) the mixed solution I, step (2) being made is put into the centrifugal 30min of whizzer that rotating speed is 6000r/min, in the phosphate buffered saline buffer that afterwards precipitation is dissolved in to pH and is 6.5, volumetric molar concentration is 0.05mol/L, then proceed to centrifugal 15min in the whizzer that rotating speed is 4000r/min, collect supernatant liquor, make soybean extraction, for subsequent use;
Step 2, take each component according to the parts by weight of described each component;
Step 3, subtilis, silicate bacteria, viride, Lactococcus lactis, actinomycetes, colloid bacillus cereus, rice Flavobacterium, yeast, azotobacter chroococcum are stirred after, progressively add proteolytic enzyme, Starch phosphorylase and cellulase, make complex liquid;
Step 4, soybean extraction is put into frequency is that the ultrasonic wave of 20kHz is processed 20min, and every processing 3min stops 15s, to the complex liquid that adds step 3 to make in soybean extraction, stirs afterwards.
Result test: the matrix color after utilizing aforesaid method to become thoroughly decomposed is chocolate, moisture content 46%, the C/N ratio of matrix is reduced to 12.3.60 ℃ of hot stages can maintain 6 days, and T=(C/N is eventually)/(C/N beginning) T<0.6.Available N-P potassium content is respectively 0.70mg/kg, 838.26mg/kg, 3592.12mg/kg.This available nutrient content can meet the needs of plant-growth completely.