CN103816368A - Extraction method and application of ginseng-containing plant composition - Google Patents

Extraction method and application of ginseng-containing plant composition Download PDF

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CN103816368A
CN103816368A CN201410091162.0A CN201410091162A CN103816368A CN 103816368 A CN103816368 A CN 103816368A CN 201410091162 A CN201410091162 A CN 201410091162A CN 103816368 A CN103816368 A CN 103816368A
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radix ginseng
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李正梅
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Xinxiang Medical University
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Abstract

The invention provides an extraction method of ginseng-containing plant composition. The ginseng-containing plant composition is prepared with a microwave extraction method by the following active pharmaceutical ingredients: 75 g of ginseng, 150 g of dried rehmannia roots, 150 g of prepared rehmannia roots, 150 g of dogwood stir-fried with wine, 300 g of Chinese yam, 150 g of oriental waterplantain rhizome, 150 g of tree peony bark, 150 g of poria, 150 g of radix ophiopogonis and 150 g of cochinchinese asparagus roots, so that the content is increased greatly, and the dosage is reduced. The invention further provides application of the ginseng-containing plant composition in preparation of a cell proliferation drug for inhibiting adrenal gland pheochromocytoma PC-12 of a rat.

Description

A kind of vegetable composition extracting method and application containing Radix Ginseng
Technical field
The present invention relates to Chinese medicine preparation technical field, be specifically related to a kind of vegetable composition extracting method and application containing Radix Ginseng.
Background technology
Containing the vegetable composition of Radix Ginseng by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g make as crude drug, record in Ministry of Public Health standard (WS3-B-0010-89), current extracting method is above ten tastes, be ground into fine powder, sieve, mix.Every 100g powder adds refined honey 80~100g, makes big honeyed pills, to obtain final product energy enriching yin boost qi, vital base and primordial QI consolidating.For a little less than deficiency of YIN gas, cough due to consumptive disease, shortness of breath and palpitation, osteopyrexia and fever, soreness of waist tinnitus, seminal emission night sweat, is hard and dry.
In prior art, not yet have containing the vegetable composition of Radix Ginseng and aspect extraction preparation, adopting the report of microwave technology, and adopt the method for beating powder, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of vegetable composition extracting method containing Radix Ginseng.
Another object of the present invention is to provide a kind of vegetable composition containing Radix Ginseng to suppress the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation.
Technical scheme: the object of the invention is to realize by following scheme:
A kind of vegetable composition extracting method containing Radix Ginseng, vegetable composition is by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill, every ball 4.5g.
Above-mentioned a kind of vegetable composition extracting method containing Radix Ginseng, in preparation method, microwave extracting power is 500W, extracts 6 minutes at every turn.
A kind of vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation, contain the vegetable composition of Radix Ginseng by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
Above-mentioned a kind of vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation, and the power of microwave extracting described in preparation method is 500W, extracts 6 minutes at every turn.
In prior art, containing the every ball 9g of vegetable composition of Radix Ginseng, each 1 ball, 2 times on the one, the every ball 4.5g of vegetable composition containing Radix Ginseng that adopts the present invention to be prepared into, but the medical material amount containing is original 2 times, therefore only need 1 ball at every turn, within 1st, take 2 times, under the condition with more active component, greatly reduce dose, the present invention is with Radix Rehmanniae, Radix Rehmanniae Preparata, wine Fructus Corni Preparata YIN nourishing is monarch drug, Radix Ginseng, Rhizoma Dioscoreae QI invigorating is ministerial drug, Rhizoma Alismatis, Cortex Moutan, Poria, Radix Ophiopogonis, Radix Asparagi vital base and primordial QI consolidating is adjuvant, jointly reach enriching yin boost qi, the effect of vital base and primordial QI consolidating.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill, every 4.5g.
Embodiment 2
Get Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
Embodiment 3
Get Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
Chinese crude drug of the present invention is pharmacopeia kind.
Embodiment 4: the experimentation data that suppresses Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation containing the vegetable composition of Radix Ginseng
1 experiment material
1.1 experiment cell strains
Clonal Rat Pheochromocytoma tumor PC-12 cell, Nanjing Medical University's Experimental Animal Center laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: the present invention contains the vegetable composition of Radix Ginseng: press embodiment 1 method preparation.
Medicinal liquid liquid storage: take the vegetable composition of 100mg containing Radix Ginseng, be dissolved in 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ l doff pipe subpackages ,-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Lot.No.100419 of Tian Hang bio tech ltd, Zhejiang); NaHCO3(Shanghai hundred million Cat.No.11810-033Lot.No.1088387 of chemical reagent company limited of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS(laboratory autogamy);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound company of Su Jing group manufactures model: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) Clonal Rat Pheochromocytoma tumor PC-12 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells is during to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after piping and druming cell, proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjusts 3 × 104/ml of concentration of cell suspension.
2) cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, and culture plate is put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to Growth of Cells situation, generally grow to 50%-70%, add the vegetable composition solution containing Radix Ginseng, continue to cultivate 24h.
4) after 24h, add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT), continue to cultivate 4h.
5) after 4h, buckle method is removed supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), sets 6 multiple holes for every group.
7) result represents the suppression ratio of cell with medicine:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, with matched group comparison, in the time that dosage reaches 5mg/ml, to Clonal Rat Pheochromocytoma tumor PC-12 cell inhibitory effect variant (P<0.05), dosage this difference in the time of 10mg/ml has significance (P<0.01), has utmost point significant difference (P<0.001) in the time that dosage reaches 15-20mg/ml.
Table 1 affects research containing the vegetable composition of Radix Ginseng to Clonal Rat Pheochromocytoma tumor PC-12 cell inhibitory effect
Figure BDA0000475832740000052
Note: with matched group comparison, * P<0.01; * P<0.001
5 experiment conclusion
Vegetable composition containing Radix Ginseng can suppress Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation, reduces the Growth of Cells number of Clonal Rat Pheochromocytoma tumor PC-12 cell, and this effect is dose dependent.

Claims (4)

1. the vegetable composition extracting method containing Radix Ginseng, it is characterized in that vegetable composition is by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill, every ball 4.5g.
2. a kind of vegetable composition extracting method containing Radix Ginseng according to claim 1, is characterized in that in preparation method, microwave extracting power is 500W, extracts 6 minutes at every turn.
3. the vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation, it is characterized in that the vegetable composition that contains Radix Ginseng is by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
4. a kind of vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation according to claim 3, it is characterized in that the power of microwave extracting described in preparation method is 500W, extracts 6 minutes at every turn.
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104997874A (en) * 2015-08-19 2015-10-28 陆茹姣 Traditional Chinese medicine for treating pheochromocytoma
CN105193715A (en) * 2015-10-23 2015-12-30 云南云龙制药股份有限公司 Preparation method for Torvosaurus liquid and application of Torvosaurus liquid
CN105194002A (en) * 2015-09-16 2015-12-30 南京正亮医药科技有限公司 Method for extracting plant composition containing echinacea and application of plant composition
CN112057548A (en) * 2020-09-29 2020-12-11 鲁南制药集团股份有限公司 Application of ginseng root-strengthening preparation in treating sperm abnormality
CN114129656A (en) * 2020-09-04 2022-03-04 山东新时代药业有限公司 New use of Ginseng radix oral liquid for preventing and treating radiation-induced lung injury
CN114246905A (en) * 2020-09-21 2022-03-29 山东新时代药业有限公司 Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating coronary heart disease and restenosis after PCI (percutaneous coronary intervention) operation
CN114246908A (en) * 2021-02-09 2022-03-29 山东新时代药业有限公司 Application of ginseng root-strengthening oral liquid in treatment of thyroid dysfunction
CN114377076A (en) * 2021-02-09 2022-04-22 山东新时代药业有限公司 Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating hyperplasia of mammary glands
CN115068547A (en) * 2022-07-22 2022-09-20 青岛市第六人民医院(青岛市传染病医院、青岛肝病研究所) Traditional Chinese medicine formula granules for treating bone metastasis tumor
CN115429847A (en) * 2020-11-27 2022-12-06 山东新时代药业有限公司 Application of traditional Chinese medicine composition in preparation of medicine for treating intractable insomnia

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1175447A (en) * 1997-07-10 1998-03-11 迟经惠 Restoration injection for nephropathy
CN1390588A (en) * 2002-08-06 2003-01-15 王凯 Composite medicine for treating leukemia
CN101327307A (en) * 2008-07-29 2008-12-24 李天德 Medicament for treating cancer and preparation method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1199736B1 (en) * 2000-04-18 2004-06-30 Mitsumi Electric Co., Ltd. Membrane switch

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1175447A (en) * 1997-07-10 1998-03-11 迟经惠 Restoration injection for nephropathy
CN1390588A (en) * 2002-08-06 2003-01-15 王凯 Composite medicine for treating leukemia
CN101327307A (en) * 2008-07-29 2008-12-24 李天德 Medicament for treating cancer and preparation method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
周恩平等: "人参固本口服液的药理实验研究", 《山东生物医学工程》 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104997874A (en) * 2015-08-19 2015-10-28 陆茹姣 Traditional Chinese medicine for treating pheochromocytoma
CN105194002A (en) * 2015-09-16 2015-12-30 南京正亮医药科技有限公司 Method for extracting plant composition containing echinacea and application of plant composition
CN105193715A (en) * 2015-10-23 2015-12-30 云南云龙制药股份有限公司 Preparation method for Torvosaurus liquid and application of Torvosaurus liquid
CN105193715B (en) * 2015-10-23 2017-12-12 云南云龙制药股份有限公司 A kind of preparation method and application of Torvosaurus liquid
CN114129656A (en) * 2020-09-04 2022-03-04 山东新时代药业有限公司 New use of Ginseng radix oral liquid for preventing and treating radiation-induced lung injury
CN114246905A (en) * 2020-09-21 2022-03-29 山东新时代药业有限公司 Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating coronary heart disease and restenosis after PCI (percutaneous coronary intervention) operation
CN112057548A (en) * 2020-09-29 2020-12-11 鲁南制药集团股份有限公司 Application of ginseng root-strengthening preparation in treating sperm abnormality
CN115429847A (en) * 2020-11-27 2022-12-06 山东新时代药业有限公司 Application of traditional Chinese medicine composition in preparation of medicine for treating intractable insomnia
CN114246908A (en) * 2021-02-09 2022-03-29 山东新时代药业有限公司 Application of ginseng root-strengthening oral liquid in treatment of thyroid dysfunction
CN114377076A (en) * 2021-02-09 2022-04-22 山东新时代药业有限公司 Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating hyperplasia of mammary glands
CN115068547A (en) * 2022-07-22 2022-09-20 青岛市第六人民医院(青岛市传染病医院、青岛肝病研究所) Traditional Chinese medicine formula granules for treating bone metastasis tumor

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