CN103816368A - Extraction method and application of ginseng-containing plant composition - Google Patents
Extraction method and application of ginseng-containing plant composition Download PDFInfo
- Publication number
- CN103816368A CN103816368A CN201410091162.0A CN201410091162A CN103816368A CN 103816368 A CN103816368 A CN 103816368A CN 201410091162 A CN201410091162 A CN 201410091162A CN 103816368 A CN103816368 A CN 103816368A
- Authority
- CN
- China
- Prior art keywords
- radix
- ginseng
- radix ginseng
- preparation
- vegetable composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000208340 Araliaceae Species 0.000 title claims abstract description 38
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 38
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 38
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 38
- 239000000203 mixture Substances 0.000 title claims abstract description 31
- 238000000605 extraction Methods 0.000 title claims abstract description 17
- 241000196324 Embryophyta Species 0.000 title abstract 4
- 239000003814 drug Substances 0.000 claims abstract description 22
- 238000002360 preparation method Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 13
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims abstract description 10
- 229940079593 drug Drugs 0.000 claims abstract description 10
- 230000004663 cell proliferation Effects 0.000 claims abstract description 8
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 45
- 235000013311 vegetables Nutrition 0.000 claims description 26
- 206010028980 Neoplasm Diseases 0.000 claims description 12
- 208000028591 pheochromocytoma Diseases 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- 239000002671 adjuvant Substances 0.000 claims description 8
- 239000006187 pill Substances 0.000 claims description 8
- 230000000274 adsorptive effect Effects 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 230000006837 decompression Effects 0.000 claims description 7
- 239000003480 eluent Substances 0.000 claims description 7
- 238000010828 elution Methods 0.000 claims description 7
- 238000004064 recycling Methods 0.000 claims description 7
- 229920005989 resin Polymers 0.000 claims description 7
- 239000011347 resin Substances 0.000 claims description 7
- 239000002994 raw material Substances 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 3
- 241000405414 Rehmannia Species 0.000 abstract 2
- 241000049624 Alisma plantago-aquatica subsp. orientale Species 0.000 abstract 1
- 244000003416 Asparagus officinalis Species 0.000 abstract 1
- 235000005340 Asparagus officinalis Nutrition 0.000 abstract 1
- 240000006766 Cornus mas Species 0.000 abstract 1
- 235000002722 Dioscorea batatas Nutrition 0.000 abstract 1
- 235000006536 Dioscorea esculenta Nutrition 0.000 abstract 1
- 240000001811 Dioscorea oppositifolia Species 0.000 abstract 1
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 abstract 1
- 240000005001 Paeonia suffruticosa Species 0.000 abstract 1
- 235000003889 Paeonia suffruticosa Nutrition 0.000 abstract 1
- 239000008186 active pharmaceutical agent Substances 0.000 abstract 1
- 201000003352 adrenal gland pheochromocytoma Diseases 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241000721047 Danaus plexippus Species 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 206010033557 Palpitations Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012738 dissolution medium Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- -1 sieve Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides an extraction method of ginseng-containing plant composition. The ginseng-containing plant composition is prepared with a microwave extraction method by the following active pharmaceutical ingredients: 75 g of ginseng, 150 g of dried rehmannia roots, 150 g of prepared rehmannia roots, 150 g of dogwood stir-fried with wine, 300 g of Chinese yam, 150 g of oriental waterplantain rhizome, 150 g of tree peony bark, 150 g of poria, 150 g of radix ophiopogonis and 150 g of cochinchinese asparagus roots, so that the content is increased greatly, and the dosage is reduced. The invention further provides application of the ginseng-containing plant composition in preparation of a cell proliferation drug for inhibiting adrenal gland pheochromocytoma PC-12 of a rat.
Description
Technical field
The present invention relates to Chinese medicine preparation technical field, be specifically related to a kind of vegetable composition extracting method and application containing Radix Ginseng.
Background technology
Containing the vegetable composition of Radix Ginseng by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g make as crude drug, record in Ministry of Public Health standard (WS3-B-0010-89), current extracting method is above ten tastes, be ground into fine powder, sieve, mix.Every 100g powder adds refined honey 80～100g, makes big honeyed pills, to obtain final product energy enriching yin boost qi, vital base and primordial QI consolidating.For a little less than deficiency of YIN gas, cough due to consumptive disease, shortness of breath and palpitation, osteopyrexia and fever, soreness of waist tinnitus, seminal emission night sweat, is hard and dry.
In prior art, not yet have containing the vegetable composition of Radix Ginseng and aspect extraction preparation, adopting the report of microwave technology, and adopt the method for beating powder, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of vegetable composition extracting method containing Radix Ginseng.
Another object of the present invention is to provide a kind of vegetable composition containing Radix Ginseng to suppress the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation.
Technical scheme: the object of the invention is to realize by following scheme:
A kind of vegetable composition extracting method containing Radix Ginseng, vegetable composition is by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill, every ball 4.5g.
Above-mentioned a kind of vegetable composition extracting method containing Radix Ginseng, in preparation method, microwave extracting power is 500W, extracts 6 minutes at every turn.
A kind of vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation, contain the vegetable composition of Radix Ginseng by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
Above-mentioned a kind of vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation, and the power of microwave extracting described in preparation method is 500W, extracts 6 minutes at every turn.
In prior art, containing the every ball 9g of vegetable composition of Radix Ginseng, each 1 ball, 2 times on the one, the every ball 4.5g of vegetable composition containing Radix Ginseng that adopts the present invention to be prepared into, but the medical material amount containing is original 2 times, therefore only need 1 ball at every turn, within 1st, take 2 times, under the condition with more active component, greatly reduce dose, the present invention is with Radix Rehmanniae, Radix Rehmanniae Preparata, wine Fructus Corni Preparata YIN nourishing is monarch drug, Radix Ginseng, Rhizoma Dioscoreae QI invigorating is ministerial drug, Rhizoma Alismatis, Cortex Moutan, Poria, Radix Ophiopogonis, Radix Asparagi vital base and primordial QI consolidating is adjuvant, jointly reach enriching yin boost qi, the effect of vital base and primordial QI consolidating.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill, every 4.5g.
Embodiment 2
Get Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
Embodiment 3
Get Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
Chinese crude drug of the present invention is pharmacopeia kind.
Embodiment 4: the experimentation data that suppresses Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation containing the vegetable composition of Radix Ginseng
1 experiment material
1.1 experiment cell strains
Clonal Rat Pheochromocytoma tumor PC-12 cell, Nanjing Medical University's Experimental Animal Center laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: the present invention contains the vegetable composition of Radix Ginseng: press embodiment 1 method preparation.
Medicinal liquid liquid storage: take the vegetable composition of 100mg containing Radix Ginseng, be dissolved in 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ l doff pipe subpackages ,-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.
1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Lot.No.100419 of Tian Hang bio tech ltd, Zhejiang); NaHCO3(Shanghai hundred million Cat.No.11810-033Lot.No.1088387 of chemical reagent company limited of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS(laboratory autogamy);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound company of Su Jing group manufactures model: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) Clonal Rat Pheochromocytoma tumor PC-12 cell carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells is during to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after piping and druming cell, proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjusts 3 × 104/ml of concentration of cell suspension.
2) cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, and culture plate is put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to Growth of Cells situation, generally grow to 50%-70%, add the vegetable composition solution containing Radix Ginseng, continue to cultivate 24h.
4) after 24h, add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT), continue to cultivate 4h.
5) after 4h, buckle method is removed supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), sets 6 multiple holes for every group.
7) result represents the suppression ratio of cell with medicine:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, with matched group comparison, in the time that dosage reaches 5mg/ml, to Clonal Rat Pheochromocytoma tumor PC-12 cell inhibitory effect variant (P<0.05), dosage this difference in the time of 10mg/ml has significance (P<0.01), has utmost point significant difference (P<0.001) in the time that dosage reaches 15-20mg/ml.
Table 1 affects research containing the vegetable composition of Radix Ginseng to Clonal Rat Pheochromocytoma tumor PC-12 cell inhibitory effect
Note: with matched group comparison, * P<0.01; * P<0.001
5 experiment conclusion
Vegetable composition containing Radix Ginseng can suppress Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation, reduces the Growth of Cells number of Clonal Rat Pheochromocytoma tumor PC-12 cell, and this effect is dose dependent.
Claims (4)
1. the vegetable composition extracting method containing Radix Ginseng, it is characterized in that vegetable composition is by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill, every ball 4.5g.
2. a kind of vegetable composition extracting method containing Radix Ginseng according to claim 1, is characterized in that in preparation method, microwave extracting power is 500W, extracts 6 minutes at every turn.
3. the vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation, it is characterized in that the vegetable composition that contains Radix Ginseng is by Radix Ginseng 75g, Radix Rehmanniae 150g, Radix Rehmanniae Preparata 150g, wine Fructus Corni Preparata 150g, Rhizoma Dioscoreae 300g, Rhizoma Alismatis 150g, Cortex Moutan 150g, Poria 150g, Radix Ophiopogonis 150g, Radix Asparagi 150g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into pill.
4. a kind of vegetable composition containing Radix Ginseng suppresses the application in Clonal Rat Pheochromocytoma tumor PC-12 cell proliferation medicine in preparation according to claim 3, it is characterized in that the power of microwave extracting described in preparation method is 500W, extracts 6 minutes at every turn.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410091162.0A CN103816368B (en) | 2014-03-12 | 2014-03-12 | A kind of vegetable composition extracting method containing Radix Ginseng and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410091162.0A CN103816368B (en) | 2014-03-12 | 2014-03-12 | A kind of vegetable composition extracting method containing Radix Ginseng and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103816368A true CN103816368A (en) | 2014-05-28 |
| CN103816368B CN103816368B (en) | 2016-02-17 |
Family
ID=50751848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410091162.0A Expired - Fee Related CN103816368B (en) | 2014-03-12 | 2014-03-12 | A kind of vegetable composition extracting method containing Radix Ginseng and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103816368B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104997874A (en) * | 2015-08-19 | 2015-10-28 | 陆茹姣 | Traditional Chinese medicine for treating pheochromocytoma |
| CN105193715A (en) * | 2015-10-23 | 2015-12-30 | 云南云龙制药股份有限公司 | Preparation method for Torvosaurus liquid and application of Torvosaurus liquid |
| CN105194002A (en) * | 2015-09-16 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing echinacea and application of plant composition |
| CN112057548A (en) * | 2020-09-29 | 2020-12-11 | 鲁南制药集团股份有限公司 | Application of ginseng root-strengthening preparation in treating sperm abnormality |
| CN114129656A (en) * | 2020-09-04 | 2022-03-04 | 山东新时代药业有限公司 | New use of Ginseng radix oral liquid for preventing and treating radiation-induced lung injury |
| CN114246905A (en) * | 2020-09-21 | 2022-03-29 | 山东新时代药业有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating coronary heart disease and restenosis after PCI (percutaneous coronary intervention) operation |
| CN114246908A (en) * | 2021-02-09 | 2022-03-29 | 山东新时代药业有限公司 | Application of ginseng root-strengthening oral liquid in treatment of thyroid dysfunction |
| CN114377076A (en) * | 2021-02-09 | 2022-04-22 | 山东新时代药业有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating hyperplasia of mammary glands |
| CN115068547A (en) * | 2022-07-22 | 2022-09-20 | 青岛市第六人民医院(青岛市传染病医院、青岛肝病研究所) | Traditional Chinese medicine formula granules for treating bone metastasis tumor |
| CN115429847A (en) * | 2020-11-27 | 2022-12-06 | 山东新时代药业有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for treating intractable insomnia |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175447A (en) * | 1997-07-10 | 1998-03-11 | 迟经惠 | Restoration injection for nephropathy |
| CN1390588A (en) * | 2002-08-06 | 2003-01-15 | 王凯 | Composite medicine for treating leukemia |
| CN101327307A (en) * | 2008-07-29 | 2008-12-24 | 李天德 | Medicament for treating cancer and preparation method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1199736B1 (en) * | 2000-04-18 | 2004-06-30 | Mitsumi Electric Co., Ltd. | Membrane switch |
-
2014
- 2014-03-12 CN CN201410091162.0A patent/CN103816368B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1175447A (en) * | 1997-07-10 | 1998-03-11 | 迟经惠 | Restoration injection for nephropathy |
| CN1390588A (en) * | 2002-08-06 | 2003-01-15 | 王凯 | Composite medicine for treating leukemia |
| CN101327307A (en) * | 2008-07-29 | 2008-12-24 | 李天德 | Medicament for treating cancer and preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 周恩平等: "人参固本口服液的药理实验研究", 《山东生物医学工程》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104997874A (en) * | 2015-08-19 | 2015-10-28 | 陆茹姣 | Traditional Chinese medicine for treating pheochromocytoma |
| CN105194002A (en) * | 2015-09-16 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing echinacea and application of plant composition |
| CN105193715A (en) * | 2015-10-23 | 2015-12-30 | 云南云龙制药股份有限公司 | Preparation method for Torvosaurus liquid and application of Torvosaurus liquid |
| CN105193715B (en) * | 2015-10-23 | 2017-12-12 | 云南云龙制药股份有限公司 | A kind of preparation method and application of Torvosaurus liquid |
| CN114129656A (en) * | 2020-09-04 | 2022-03-04 | 山东新时代药业有限公司 | New use of Ginseng radix oral liquid for preventing and treating radiation-induced lung injury |
| CN114246905A (en) * | 2020-09-21 | 2022-03-29 | 山东新时代药业有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating coronary heart disease and restenosis after PCI (percutaneous coronary intervention) operation |
| CN112057548A (en) * | 2020-09-29 | 2020-12-11 | 鲁南制药集团股份有限公司 | Application of ginseng root-strengthening preparation in treating sperm abnormality |
| CN115429847A (en) * | 2020-11-27 | 2022-12-06 | 山东新时代药业有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for treating intractable insomnia |
| CN114246908A (en) * | 2021-02-09 | 2022-03-29 | 山东新时代药业有限公司 | Application of ginseng root-strengthening oral liquid in treatment of thyroid dysfunction |
| CN114377076A (en) * | 2021-02-09 | 2022-04-22 | 山东新时代药业有限公司 | Application of traditional Chinese medicine composition in preparation of medicine for preventing and treating hyperplasia of mammary glands |
| CN115068547A (en) * | 2022-07-22 | 2022-09-20 | 青岛市第六人民医院(青岛市传染病医院、青岛肝病研究所) | Traditional Chinese medicine formula granules for treating bone metastasis tumor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103816368B (en) | 2016-02-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103816368B (en) | A kind of vegetable composition extracting method containing Radix Ginseng and application | |
| CN103784738B (en) | A kind of vegetable composition extracting method containing Lepidinm meyenii Walp and application | |
| CN103599292B (en) | A kind of preparation method of Guishaoliujun Pill and application | |
| CN103860802A (en) | Preparation method and application of Jieguqili tablet | |
| CN105030868A (en) | Extraction method for plant composition containing silybum marianum and application of plant composition | |
| CN103520446B (en) | A kind of preparation method of the Zijin ball that bonesets and application | |
| CN103656600B (en) | A kind of preparation method of 12 WENJING WAN and application | |
| CN104887814A (en) | Preparation method and application of Shenqi Wuweizi capsules | |
| CN103690751A (en) | Application and preparation method of Shuxinning tablets | |
| CN103520430A (en) | Preparing method and application of pill containing seven drugs for kidney deficiency | |
| CN103536733B (en) | Preparation method and application of rhizoma cyperi pill with ten precious materials | |
| CN103638489B (en) | Preparation method and application of kidney-tonifying pills | |
| CN105055587A (en) | Extraction method and application of plant composition containing ginkgo leaves | |
| CN103638487B (en) | A kind of preparation method of Babao Ruisheng pill and application | |
| CN103610857B (en) | A kind of preparation method and application of returning red pill for traumatic injuries | |
| CN105250373A (en) | Extraction method and application of plant composition containing salvia miltiorrhiza | |
| CN104906351A (en) | Preparation method and application of capsule for nourishing kidney essence and benefiting brain | |
| CN105106570A (en) | Extraction method and application of garlic-containing plant combination | |
| CN104887905A (en) | Preparation method for six-ingredient rehmannia pills and application thereof | |
| CN103721071B (en) | A kind of preparation method of YULU Baofei bolus and application | |
| CN105194002A (en) | Method for extracting plant composition containing echinacea and application of plant composition | |
| CN103655923B (en) | A kind of preparation method of Auricularia SHUJIN WAN and application | |
| CN103623212B (en) | A kind of preparation method and application consolidating ball | |
| CN105106331A (en) | Extraction method and application of plant composition containing oenothera biennis | |
| CN105079162A (en) | Extraction method and application of honeysuckle-containing herbal composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Guo Zhigang Inventor after: Yan Xiaoxiao Inventor after: Li Yinghong Inventor after: Zhao Linjing Inventor after: Zhong Jiateng Inventor after: Guo Zhifang Inventor after: Cui Liusu Inventor before: Li Zhengmei |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160121 Address after: No. 601 Xinxiang City, Henan province Jinsui road 453003 Applicant after: Xinxiang Medical College Address before: 211200 Rongchang City, Nanjing province Lishui Yong Yang Town, garden building, room 105, room 27, No. Applicant before: Li Zhengmei |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160217 Termination date: 20170312 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |