CN103816214B - Application of the peanut root extract in the medicine or food for preparing preventing and treating hyperplasia of prostate - Google Patents
Application of the peanut root extract in the medicine or food for preparing preventing and treating hyperplasia of prostate Download PDFInfo
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- CN103816214B CN103816214B CN201410103892.8A CN201410103892A CN103816214B CN 103816214 B CN103816214 B CN 103816214B CN 201410103892 A CN201410103892 A CN 201410103892A CN 103816214 B CN103816214 B CN 103816214B
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- peanut
- prostate
- root extract
- roots
- medicine
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- 235000017060 Arachis glabrata Nutrition 0.000 title claims abstract description 37
- 235000010777 Arachis hypogaea Nutrition 0.000 title claims abstract description 37
- 235000018262 Arachis monticola Nutrition 0.000 title claims abstract description 37
- 235000020232 peanut Nutrition 0.000 title claims abstract description 37
- 241001553178 Arachis glabrata Species 0.000 title claims abstract description 21
- 239000003814 drug Substances 0.000 title claims abstract description 21
- 206010004446 Benign prostatic hyperplasia Diseases 0.000 title claims abstract description 18
- 235000013305 food Nutrition 0.000 title claims abstract description 10
- 240000005781 Arachis hypogaea Species 0.000 claims abstract description 16
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 238000010992 reflux Methods 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 3
- 239000012141 concentrate Substances 0.000 claims abstract 2
- 239000012153 distilled water Substances 0.000 claims abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 7
- 229940079593 drugs Drugs 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 2
- 241000700159 Rattus Species 0.000 abstract description 17
- 210000002307 Prostate Anatomy 0.000 abstract description 10
- 231100000486 side effect Toxicity 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 229960001712 Testosterone Propionate Drugs 0.000 description 2
- PDMMFKSKQVNJMI-BLQWBTBKSA-N Testosterone propionate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](OC(=O)CC)[C@@]1(C)CC2 PDMMFKSKQVNJMI-BLQWBTBKSA-N 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 239000003182 parenteral nutrition solution Substances 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 102100013894 BCL2 Human genes 0.000 description 1
- 108060000885 BCL2 Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 210000004907 Glands Anatomy 0.000 description 1
- 206010020718 Hyperplasia Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003098 androgen Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000002424 anti-apoptotic Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000020828 fasting Nutrition 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002045 lasting Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000001717 pathogenic Effects 0.000 description 1
- 244000052769 pathogens Species 0.000 description 1
- WRLGYAWRGXKSKG-UHFFFAOYSA-M phenobarbital sodium Chemical compound [Na+].C=1C=CC=CC=1C1(CC)C(=O)NC([O-])=NC1=O WRLGYAWRGXKSKG-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Abstract
The present invention relates to medicine food technical field, specifically application of the peanut root extract in the medicine or food for preparing preventing and treating hyperplasia of prostate, the preparation method of peanut root extract decoction：Roots of Peanut is cleaned first and dries smashing, then dissolved with extract solution, boiling reflux, secondly cooling filters, filtrate is concentrated, finally concentrate is dissolved with distilled water, make up to pharmaceutical grade, the present invention passes through SD rat tests, demonstrate and take the peanut root extract decoction for reaching doses, prostate index can be effectively reduced, suppress hyperplasia of prostate, the present invention is possible to be developed to a kind of curative effect height for Roots of Peanut, the preventing and treating hyperplasia of prostate of Small side effects provides a kind of new medicament sources, the application of Roots of Peanut is expanded, and because Roots of Peanut is originally crops, to the Small side effects of human body, it is extensive with plantation, the advantage of abundance, for the plantation of peanut, increasing peasant income are by with good economic benefit and social benefit.
Description
Technical field
The present invention relates to pharmaceutical technology field, specifically peanut root extract prepare the medicine of preventing and treating hyperplasia of prostate or
Application in food.
Background technology
Benign prostatic hyperplasis (Benign Prostatic Hyperplasia, BPH) is elderly men common disease, more
Morbidity, with the aging of population in the world, its incidence of disease constantly increases.So far, BPH pathogenesis is not completely clear
Chu.There is research to think, BPH generation is in addition to relevant with the unconventionality expression of amplified gene, apoptogene and anti-apoptotic genes expression, very
May be also relevant with the interaction of male, estrogen unbalance and various growth factor.The elderly's androgen levels resist after declining
Apoptogene bcl-2 high expression causes prostatic cell apoptosis to decline the principal element for being likely to that BPH occurs.
At present, operative treatment BPH is evident in efficacy, but certain damage is caused to patient.Seek drug therapy, particularly plant
Thing medicine is treated, and emphasis is overall with it, controls that benefit has concurrently, Small side effects, curative effect stablize the concern that lasting advantage enjoys people.
The effect of " single side's taste " the Roots of Peanut Chinese medicine among the people that is known as says, Roots of Peanut has qi-restoratives by oneself, but applied to preparing
Prevent and treat and still do not had been reported that in the medicine or food of hyperplasia of prostate.
The content of the invention
It is an object of the invention to which peanut root extract is applied in the medicine or food of preventing and treating hyperplasia of prostate is prepared,
Peanut root extract decoction is made up of following steps：
Step A：The fresh Roots of Peanut for throwing aside field is collected, cleans and dries smashing；
Step B：By the use of the product obtained by the ethanol that volume fraction is 50-80% as extractant dissolving step A, boiling reflux
For a period of time；
Step C：Product obtained by step B is cooled down and filtered, takes filtrate；
Step D：Product at reduced pressure obtained by step C is distilled, obtains down concentrate；
Step E：Product obtained by step D is dissolved with distilled water, obtains pharmaceutical grade decoction.
Further improve is：The mass figures of Roots of Peanut described in the above method and the volumetric quantities of alcohol solvent it
Than for 1: 2.
Further improve is：The liquor strength obtained in the step E is equivalent to crude drug 10g/ml.
By the pharmacological evaluation to SD (Sprague-Dawley) rat, additionally filled to rat and raise peanut root extract decoction
Reach the rat of doses and do not fill to raise peanut root extract decoction or fill and raise low big of peanut root extract liquid dose
Mouse is compared, and the prostate index that the rat that feeding peanut root extract decoction reaches doses is additionally filled to rat is more preferable.
The present invention has expanded the new medical applications of peanut root extract, is provided to prevent and treat the medicine of hyperplasia of prostate or food
A kind of new medicament sources, and peanut root extract safety and low toxicity, Small side effects, raw material sources enrich, to make full use of
The medical value of this crop of peanut is laid a good foundation.
Embodiment
In order to deepen the understanding of the present invention, the present invention is described in further detail below in conjunction with embodiment, the implementation
Example is only used for explaining the present invention, and protection domain is not formed and limited.
Embodiment 1：The preparation of peanut root extract decoction
Step A：The fresh Roots of Peanut for throwing aside field is collected, cleans and dries smashing；
Step B：The Roots of Peanut powder 500g in step A is taken, extraction is used as by the use of the ethanol 1000mi that volume fraction is 60%
Agent is dissolved, boiling reflux 30min；
Step C：Product obtained by step B is cooled to 20 DEG C of suction filtrations, takes filtrate；
Step D：Product at reduced pressure obtained by step C is distilled, obtains down concentrate；
Step E：Product obtained by step D 50ml distilled waters are dissolved, obtain pharmaceutical grade decoction.
The peanut root extract decoction of gained is equivalent to crude drug 10g/ml.
Embodiment 2：Pharmacological testing
Test material：
1. animal：
Male SD rat, body weight 180-200g, no-special pathogen, provided by Jiangxi College of Traditional Chinese Medicine animal center.
2. medicine and reagent：
Peanut root extract decoction obtained by embodiment 1, testosterone propionate parenteral solution (TP) 25mg/ml, Shanghai GM medicine
Industry limited company produces.
3. test method：
SD rats are randomly divided into 5 groups, every group 12.
Normal group：Diet, not medication.
Model group：TP50mg/kg, 3 times a week (Monday, three, five), continuous 14 days is subcutaneously injected.
Treatment group：It is divided into 3 groups, not only follows model group to inject TP50mg/kg, it is just (all 3 times a week also from first day
First, three, five), it is continuous to fill the peanut root extract decoction for raising various dose.Wherein, low dose group：Peanut root extract decoction
1ml/kg；Middle dose group：Peanut root extract decoction 5ml/kg；High dose group：10ml/kg.
15th day, fasting 10h, each group rat gave excessive sodium phenobarbital (100mg/kg) execution, separates rat
Prostate, rat body weight and prostate wet weight are weighed with assay balance respectively, calculate prostate index.
Data and statistical disposition：Parameters represent that significant difference uses software spss10.0 progress sides with X ± S
Difference is analysed.
Model group prostate wet weight and index increase, the statistically significant (P of difference compared with normal group<0.01) it is, middle and high
For dosage group compared with model group, difference has statistical significance (P<0.05), compared with model group, difference does not have low dose group
Standby statistical significance (P>0.05).
The influence dosage group (X ± S, n=12) of each group SD rat prostates weight in wet base of table 1 and index
4. result of the test：
In addition to normal group, remaining each group SD rats have injected testosterone propionate parenteral solution, and this can cause rat forefront
Gland hyperplasia, establish benign prostatic hyperplasia in rats animal model.
Result of the test：According to the data in table 1, the prostate wet weight of rat substantially compares model group in middle and high dosage group
Reduce, prostate index also reduces, and the rat of low dose group compares model group, and prostate index does not have significant changes.
Claims (2)
1. application of the peanut root extract in the medicine or food for preparing preventing and treating hyperplasia of prostate, peanut root extract decoction
Preparation method is as follows：
Step A：The fresh Roots of Peanut for throwing aside field is collected, cleans and dries smashing；
Step B：By the use of the product obtained by the ethanol that volume fraction is 50-80% as extractant dissolving step A, boiling reflux one
The section time；
Step C：Product obtained by step B is cooled down and filtered, takes filtrate；
Step D：Product at reduced pressure obtained by step C is distilled, obtains concentrate；
Step E：Product obtained by step D is dissolved with distilled water, obtains pharmaceutical grade decoction, the pharmaceutical grade liquor strength phase
When in crude drug 10g/ml.
2. the answering in the medicine or food for preparing preventing and treating hyperplasia of prostate of the peanut root extract according to claim 1
With, it is characterised in that：The ratio between the mass figures of Roots of Peanut described in the above method and the volumetric quantities of alcohol solvent are 1: 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410103892.8A CN103816214B (en) | 2014-03-17 | Application of the peanut root extract in the medicine or food for preparing preventing and treating hyperplasia of prostate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410103892.8A CN103816214B (en) | 2014-03-17 | Application of the peanut root extract in the medicine or food for preparing preventing and treating hyperplasia of prostate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103816214A CN103816214A (en) | 2014-05-28 |
| CN103816214B true CN103816214B (en) | 2018-02-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ma Yongchao Inventor after: Guo Xiaohui Inventor after: Chen Honglian Inventor after: Bei Zhanyu Inventor after: Luo Xiaobing Inventor after: Wang Min Inventor after: Zhao Lifang Inventor after: Chen Yongfang Inventor before: Bei Zhanyu Inventor before: Cui Yanbing Inventor before: Wang Wenbao Inventor before: Luo Hailan Inventor before: Lu Jianfeng |
|
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180209 Termination date: 20190317 |