CN103777336B - Microoptic capture device fluorescence accessory module, pedestal and the device - Google Patents
Microoptic capture device fluorescence accessory module, pedestal and the device Download PDFInfo
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- CN103777336B CN103777336B CN201310047142.9A CN201310047142A CN103777336B CN 103777336 B CN103777336 B CN 103777336B CN 201310047142 A CN201310047142 A CN 201310047142A CN 103777336 B CN103777336 B CN 103777336B
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Abstract
A kind of microoptic capture device fluorescence accessory module, the fluorescence accessory module includes：One be formed with a predetermined observation position pedestal；One correspondence links and covering pedestal and the shade for surrounding out a light masking chamber jointly with pedestal；And a light source assembly, the light source component of plural number offer low angle exciting light is provided；Wherein, at least one of shade and pedestal are formed with breach and a guidance part that at least one supplies carrier to pass in and out, and guidance part is to extend along a predetermined direction, moved for guiding carrier along predetermined direction；And the light emission direction of light source component that low angle is excited is to concentrate the predetermined observation position towards pedestal.
Description
【Technical field】
The present invention is on a kind of fluorescence accessory module, especially a kind of microoptic capture device fluorescence accessory mould
Group, pedestal and the device.
【Background technology】
Fluorescence microscopy is except the cell analysis in for biological study with addition to tracking, further extending to industrial inspection in recent years
The essence application such as survey, counterfeit banknote identification and criminal identification so that the importance of fluorescence microscopy image capture is gradually lifted, conventional
Fluorescence microscope, mainly by the light irradiation of a high frequency on the thing to be observed with fluorescent characteristic, such as banknote it is false proof
Line, or the doubtful bloodstain in punishment case scene position, use and inspire the fluorescence compared with low frequency, along with appropriate filter group
Close, just clearly can watch or capture the fluoroscopic image of above-mentioned banknote or bloodstain；In addition, in the field of bioscience, many is ground
It is to turn to plant for gene to study carefully, and for ease of observation, the gene of implantation can often produce fluorescin, and the fluorescence by thing to be observed is anti-
Ying Youwu, confirms the success or failure of gene insertion, and can be to turning to plant successfully biological further further investigation.
Although fluorescence microscope, which has, uses upper convenience, it can effectively assist such as researcher's observation that there is fluorescence
The determinand of reaction；However, forcing the observer for requiring fluorescence reaction to abandon existing microscope in research department, purchase again
The high fluorescence microscope of price is bought, and does not meet general expectation, especially microscope price and its performance correlation is very high, merely
The quality of eyeglass number and manufacturing process, is just enough significantly to influence its price, if having purchased high performance general optics
Microscope, unlikely separately purchases a same high performance fluorescence microscope easily.Therefore, how by additional accessory, one is allowed
As light microscope can also be transformed into the fluorescence microscope of lockable fluorescent characteristic easily, just turn into the emphasis studied of the present invention.
For ease of analyzing and observing, the related Bioexperiment of current fluorescence is usually used by ultraviolet light or dark blue
Light excites and discharged the fluorescin of blue light or green glow.Therefore, a kind of existing gram difficult servicing unit 7 is as shown in figure 1, be direct
Ultraviolet source 72 is set with such as two front ends of snake belly tube 71, and allows user voluntarily to curve snake belly tube 71 and captures appropriate illumination angle
Degree, exposes to the culture dish 6 for being loaded with fluorescence thing to be seen, uses generation luminescence generated by light, but due to the structure of snake belly tube 71, when
Light microscope object lens 92 and object distance only two or three centimeter when, snake belly tube 71 will be difficult to bend into from this small space,
So that angle can not precision adjust, often not as expected, and brightness in illumination range is not easy to uniformly lighting angle so that
The image of acquisition can not be analyzed by accurate quantification.
Especially, there is a kind of fluorogene zebra fish by numerous studies in recent years, be primarily due to such zebra fish numerous
The amount of growing is big and the breeding cycle is short, can produce a large amount of offsprings, and with the characteristic such as subhuman tract, in addition it is expanded extremely
The correlative studys such as disease research, drug screening, poisonous substance test, but zebra fish is still living body biological in an experiment, so
When carrying out above-mentioned fluorescence microscopy experiment, when thing to be observed is such as fluorescence zebra fish or maggot, object may be moved constantly
Position, also dissolved oxygen is not enough and dead in an experiment during zebra fish movable in small water droplet is also possible to because of water droplet, it is necessary to simultaneously
Operating time and experimental situation are considered to biological influence, uneven small range illumination is even more to make us being fed up with, if operation
Unskilled or bad luck, is also easy for being oriented to abortive experiment result.
Therefore, from a kind of uniform illumination and easy to operate rapid fluorescence accessory module, researcher is allowed in observed and recorded
During, light source, and appropriate covering outer interference light are operated without constantly diverting one's attention, allows experimentation smoothly and is easy to observation and record
In record, live body finite lifetime that more can be on carrier, experimental study is readily accomplished, on the one hand lifting experiment speed, it is easy to
Larger amount of data information is obtained, another aspect also lifts the quality of experimental study, allows success rate to increase.
In summary, the present invention provides a kind of more excellent microoptic capture device fluorescence accessory module, pedestal
And the device, being on the one hand easily promoted to general microscope or microcamera can be in response to fluorescence Research Requirements；Another aspect
While structure, lifting operation convenience is simplified, the quality of microexamination record will not be also sacrificed, and proposition one is very steady
Fixed and succinct operating platform, meets miscellaneous experimental applications requirement, while having cheap advantage, possesses it
Unrivaled cost performance.
【The content of the invention】
It is an object of the present invention to provide a kind of fluorescence accessory module with high compatibility, one can be linked easily
As the microoptic capture device such as light microscope or microcamera, comply with fluorescence operation demand, increase microoptic
The application of capture device.
Another object of the present invention is the fluorescence accessory module for providing a kind of uniform illumination, allows animated thing to be observed
Observation scope will not be departed from easily, the success rate of research experiment is improved.
Another object of the present invention is the fluorescence accessory module for providing a kind of illumination-constant, allows observation record is quantitative to be melted into
For possibility, the quality of lifting microexamination record is used.
It is yet a further object of the present invention to provide a kind of fluorescence accessory module of modular structure so that research observation
Operation simplifies, and forms the success rate of a stabilization and the experiment of friendly operating platform, further Study of Lifting.
A further object of the present invention is to provide a kind of light source member that there is the low angle that can adjust light emission direction to excite
The fluorescence accessory module of part, excitation beam can be conditioned in response to the position of fluorescence thing to be observed, lifts the convenience used.
The further object again of the present invention is a kind of fluorescence accessory module with collecting optical opticas component of offer, makes light source
Predetermined observation scope is correctly converged to, makes the scope being observed brighter and uniform illumination.
More one purpose of the present invention is to provide a kind of fluorescence accessory module with shade, can cover extraneous strong light and
The interference of veiling glare, the quality of lifting fluorescence microscopy imaging.
A further object of the present invention is a kind of fluorescence accessory module of more radiating effect of offer, makes light source in energy
The heat energy given out during conversion lifts the efficiency and precision of experiment away from experiment body, interference of the reduction equipment to experiment body.
For up to above-mentioned purpose, the present invention discloses a kind of microoptic capture device fluorescence accessory module, pedestal and the dress
Put, be to observe the fluorescence thing to be observed being carried on a carrier, and shape on the carrier for foregoing microoptic capture device
Into there is at least one predetermined supporting region, the fluorescence accessory module includes：One be formed with a predetermined observation position pedestal；One
Individual correspondence links and covering pedestal and the shade for surrounding out a light masking chamber jointly with pedestal；And a light source assembly,
The light source component that the low angle of the one of shade and pedestal is excited is arranged at including plural number；Wherein, shade and at least the one of pedestal
Person is formed with the breach and a guidance part that at least one supplies carrier to pass in and out, and guidance part is to extend along a predetermined direction, supply
Guiding carrier is moved along predetermined direction；And the light emission direction of above-mentioned light source component is to concentrate the predetermined observation bit towards pedestal
Put.
Above-mentioned fluorescence accessory module is installed in after microoptic capture device, can obtain disclosed micro-
Optics capture device, including：One fuselage and the object lens for being installed in the fuselage；One is arranged at the fluorescence accessory module of the fuselage,
The fluorescence accessory module is the fluorescence thing to be observed being carried on a carrier for foregoing micro- sem observation, and shape on the carrier
Into there is at least one predetermined supporting region, including：One be formed with a predetermined observation position pedestal；One correspondence links and covered
Lid pedestal and the shade for surrounding out a light masking chamber jointly with pedestal；And a light source assembly, including plural number is arranged at
The light source component that shade and the low angle of the one of pedestal are excited；Wherein, at least one of shade and pedestal are formed with least one
The breach and a guidance part of individual confession carrier turnover, guidance part are extended along a predetermined direction, for guiding carrier along in advance
Determine direction movement；And the light emission direction of above-mentioned light source component is to concentrate the predetermined observation position towards pedestal.
It is as disclosed after above-mentioned fluorescence accessory module function is combined with microoptic capture device with pedestal
Microoptic capture device pedestal, including：One be formed with a predetermined observation position frame body, this body more wraps
Include a pedestal；One correspondence links and covers said base and cover chamber for surrounding out a light jointly with said base
Shade；And a light source assembly, the light source component of plural number offer low angle exciting light is provided；Wherein, the shade and the pedestal
At least one be formed with least one breach and a guidance part for supplying above-mentioned carrier to pass in and out, the guidance part is pre- along one
Determine direction extension, moved for guiding above-mentioned carrier along the predetermined direction；And the light emission direction of said light source element is to concentrate
Towards the above-mentioned predetermined observation position of said base.
Microoptic capture device fluorescence accessory module, pedestal and the device disclosed according to the present invention, the present invention is carried
The fluoroscopic assist module of confession has the characteristic for simplifying operation, and can be connected easily in known optical microscope or microcamera
Deng microoptic capture device, increase the application of microoptic capture device, meet the expectation of consumer, and it is modular
Design can allow excitation source to provide user adjustment luminous position, not have the sacrifice quality of fluorescence microscopy and asking for efficiency equally
Topic, and the micro- information of height of fluorescence thing to be observed can be obtained, reach all above-mentioned purposes.
【Brief description of the drawings】
Fig. 1 is a kind of known optical servicing unit side view.
Fig. 2 is the fluorescence accessory module profile of the first preferred embodiment of the invention.
Fig. 3 is the stereogram that Fig. 2 fluorescence accessories module is applied to microoptic capture device.
Fig. 4 is the part-structure figure of Fig. 2 fluorescence accessory modules.
Fig. 5 is the stereogram that Fig. 2 fluorescence accessories module is applied to stereomicroscope.
Fig. 6 is the fluorescence accessory module side view that the second preferred embodiment of the invention illustrates another embodiment breach.
Fig. 7 is the fluorescence accessory module side view that the 3rd preferred embodiment of the invention illustrates another embodiment breach.
Fig. 8 is to illustrate the structure chart of base form in Fig. 7.
Fig. 9 is the fluorescence accessory module profile of the 4th preferred embodiment of the invention.
Figure 10 is the fluorescence accessory module profile of the 5th preferred embodiment of the invention.
Figure 11 is the fluorescence accessory module profile of the 6th preferred embodiment of the invention.
Figure 12 is the microoptic capture device pedestal profile of the 7th preferred embodiment of the invention.
Figure 13 is the stereogram of Figure 12 microoptic capture device pedestal partial structures.
Figure 14 is the 8th preferred embodiment microoptic capture device pedestal partial structure figure of the invention.
Figure 15 is the microoptic capture device pedestal profile of the 8th preferred embodiment of the invention.
【Main element symbol description】
Fluorescence accessory module 1,13Frame body 106、107
Pedestal 11,111、112、115、114、116Shade 12,121、122、126、127
Perforation 121,1216Light source assembly 13,132、135、136、137
Light source component 131,1314、1315、1316、1317
Regulating part 132,1324、1326Photocon 1335、1337
Light covers chamber 14,145、146Breach 15,151、152、156
Guidance part 16,166Optical filter 17,173、176
Collecting optical opticas component 18,186Foldable parts 193
The servicing unit 7 of culture dish 6
The ultraviolet source 72 of snake belly tube 71
【Embodiment】
For the present invention foregoing and other technology contents, feature and effect, in the preferable of following cooperation Figure of description
In the detailed description of embodiment, can clearly it present；Same or analogous element, by with similar label sign.
As shown in Figures 2 to 4, the fluorescence accessory module 1 in this example captures dress with releasing the microoptic that example is light microscope 9
Put collocation to use, light microscope 9 includes fuselage 91, object lens 92 and plummer 93, and fluorescence accessory module 1 is to be installed in light
Between the object lens 92 and plummer 93 of learning microscope 9.The one side of fluorescence accessory module 1 can provide appropriate excitation source, thorn
The fluorescence thing 941 to be observed that swash has fluorescence reaction in carrier 94 sends fluorescence, another aspect can suitably covering outer it is miscellaneous
Light, lifting fluorescent signal allows observation result to be more easy to interpretation relative to the ratio of noise.Certainly, also can be as shown in figure 5, by fluorescence
Accessory module 1 is applied to stereomicroscope 91In, it cannot be only used for the two-dimentional magnified image of observed objects, moreover it is possible to see object
Depth.
Carrier 94 in this example is to be formed with three depressions by taking glass carrier as an example, and in glass carrier, as pre-
Supporting region 942 is determined for as described in above-mentioned example, allowing the zebra fish with fluorogene to be placed on the depression for filling a small amount of aqueous
In, as the fluorescence thing 941 to be observed in this example.Certainly, the fluorescence thing to be observed to observation be compared with mcroorganism such as maggot,
Also the carrier for adopting large-size can be changed, and only stay single depression, for preventing it from departing from predetermined illumination model during observation
Enclose.
The fluorescence accessory module 1 of the present invention mainly includes：Pedestal 11, shade 12 and light source assembly 13.Wherein, pedestal 11
It is to be seated on the plummer 93 of light microscope 9 in this example, and is formed with a predetermined observation position, specifically,
The formation of pedestal 11 is provided with the breach 15 and guidance part 16 of the turnover of carrier 94；As shown in figure 4, the surface of pedestal 11 can be by example
Such as fine turning lathe processing, the conduit being recessed together in middle body formation so that two ends of the pedestal 11 along conduit are formed with two
Away from each other, the breach 15 of depression, the channel width in the line direction of breach 15 just meets the size of carrier 94, makes carrier 94
It can be placed in conduit, and be slid along conduit direction, allow researcher's automatic moving carrier 94 to be directed to optimal sight
Location is put, certainly, and guiding carrier 94 is not limited to the conduit using this example, therefore is referred to as leading by this class formation in the present invention
Draw portion 16.
Shade 12 is a kind of semicircular cover body in this example, and is made of that, with alternatively non-transparent material, effectively will use
Extraneous ambient light isolation, it is to avoid existing bias light is irradiated into object lens 92 in environment, causes meaningless noise to disturb, in order to avoid
The image data to be captured is allowed to be more difficult to interpretation.Certainly, the form of shade 12 is not limited when actually implementing with illustrating, it is only necessary to
The predetermined observation position of pedestal 11 is coated wherein, and a light masking chamber 14 can be surrounded out jointly with pedestal 11；
The top of shade 12 is then formed with the perforation 121 that an offer object lens 92 are passed, and uses offer image strip to microscope, even
This chadless can be configured as collimation and be used, and it is not that the light beam directly injected from predetermined observation position can not lead to allow all
Cross.
Light source assembly 13 is to be provided with multiple light sources element 131 on the internal face of shade 12 in this example, due to general use
To excite the light beam of fluorescence, the part being really absorbed by the phosphor is less, and the ratio directly reflected is higher, if light source is set
Put close to perforation 121 at, will cause substantial amounts of reflected light enter microscopical object lens, thus the observation of interference experiment result or
Record.So the present invention is when setting light source component 131, the direction of selection light source and predetermined observation position line, relative to base
Angle between 11 bearing of trends of seat is less than 45 degree, therefore, light source component especially is defined as into the light source component that low angle is excited
131, especially this example is as light source component 131 using long lifespan, the light emitting diode (LED) of stable luminescence；Also, LED can
In response to the space size of fluorescence accessory module 1, there can be the configuration mode of multi-form or quantity, and be not limited to example of the present invention and release
Design structure.
Because the fluorescence thing to be observed in this example is that by taking zebra fish as an example, thing to be observed can depart from optimum illumination model at any time
Enclose, once the subregion of predetermined observation position has illumination inadequate or the even situation of uneven illumination, zebra fish enters the region
The difficulty of observation will be caused；To allow excitation source efficiently and uniformly to irradiate whole above-mentioned predetermined observation position, Huo Zheyin
Fluorescence thing size to be observed is answered, light source assembly 13 can further include the regulating part 132 of one group of regulation LED location,
LED irradiating angle is finely tuned by regulating part 132, for operator as shown in solid line and dotted line in figure, each LED edges are voluntarily controlled
A track, in the movement of the surface of shade 12, offer more preferably light angle is used.
Further, there is certain dispersion angle because LED is luminous, to allow the luminous beam of excitation source can be efficiently
Cover above-mentioned predetermined observation position, fluorescence accessory module 1 further includes one group of collecting optical opticas component 18, be to be molded in this example
The conduit both sides of pedestal 11, allow each LED to send slightly in the excitation beam of dispersion angle towards the convergent convex lens of carrier 94
Exemplified by so that the luminous beam of excitation source institute can be converged to parallel by dispersion angle, and more accurately uniformly dispersing is waited to see to fluorescence
The institute of thing 941 region that may be present is surveyed, certainly, this structure is not limited to convex lens, and being familiar with this technology person can also be further
Collecting optical opticas component and guidance part are done into an integration, two lens are embedded at a certain distance from smooth base-plates surface, by chance shape
Into the slide guide track of carrier, collecting optical opticas component is not possessed the function of light harvesting singly, be more provided simultaneously with effect of guiding.
Can effectively to stop that veiling glare and bias light enter object lens 92, fluorescence accessory module 1 further includes one in this example
Group is arranged at the front of object lens 92, the optical filter 17 to cover object lens 92, is greatly reduced and does not meet this time experiment specific fluorescent ripple
The penetrance of long component, it is allowed to which specific fluorescent wavelength components are largely penetrated, uses lifting signal noise ratio, meets experimental result
Detection and analysis demand, for example, when excitation source be ultraviolet light, and fluorescence thing 941 to be observed send fluorescence for blue light when,
Optical filter 17 may filter that and absorb the ultraviolet light for being directly mirrored and returning to object lens 92, it is to avoid the strong light lid of reflection crosses what is observed
Blue light image, and enter object lens 92 only for the light of the specific wavelength of fluorescence；In addition, optical filter is according to detection demand, Ke Yishi
The optical filtering microscope group that plural optical filter is constituted.
Further, second embodiment of the invention is as shown in fig. 6, pedestal 111Upper surface be in plane and non-notch
And the construction of slide guide track, the breach 15 in this example1Then it is formed at shade 121Bottom, therefore when shade 121It is engaged in pedestal
111When, you can formed such as the guidance part of previous embodiment, to provide guidance function during carrier turnover.It is of course also possible to
As shown in Fig. 7 3rd embodiments, via pedestal 112With shade 122The breach of half is respectively formed with, and is bonded to each other as one
Complete breach 152Combination, thereby formed one have guide rails pedestal 112, the carrier bigger for passing in and out volume,
Increase the application elasticity of fluorescence accessory module, specifically, 3rd embodiment only forms single breach, in the opposite side of schema
Face is then completely enclosed to be existed without any breach, by pedestal 112On guide rails, can equally reach guiding carrier
Purpose.
As shown in figure 8, example is interpreted as low angle LED light source assembly 13 in this example2It is to be arranged at pedestal 112In, and with six
Root can synchronization lifting support column set up six groups of laser diodes respectively exemplified by so that light source assembly 132Can be relative to pedestal
112Move up and down, change the angle of illumination by height change, certainly, to make the luminous of laser diode can be effective
Diffusion, is both provided with the optical lens (not shown) of one group of diffusion in front of each laser diode, and by for example
Light beam after diffusion is converged to a less dispersion angle by the collecting optical opticas component being arranged on carrier again.Certainly exist
In the case of not influenceing LED setting angles, light source assembly may also set up on shade, hold the facility for seeing setting and the need used
Ask to be changed.
In addition, the structure that shade and pedestal are formed in one also is feasible scheme, fourth embodiment of the invention such as Fig. 9 institutes
Show, be that one section of long-shaped cavity is molded with such as aluminium extruded, while as pedestal and shade, in addition to being formed with one except top and import and export,
Two sides outside towards figure then can be completely obscured with shield, this fluorescence accessory module 13Further include one be pivotally coupled to it is above-mentioned enter
The foldable parts 19 of outlet port3, allow foldable parts 193Carrier 94 is used as during unlatching3Enter out position, and cover in import and export
When, in addition to shading is assisted, optical filter 17 can also be provided as3Erection be used.Similarly, as shown in Figure 10, low angle is excited
Light source component 1314Can be by the regulating part 132 in this example4Pivot, by LED lighting angle with such as 45 degree of light emitting anger
Degree, is turned and pedestal 114The level angle being parallel to each other, uses offer more preferably light angle.
If in addition, situations such as running into the observation that is biological temperature sensitive or needing long duration of experiment, and light masking chamber
Cladding design unfavorable light emitting source again heat energy diverging, the obstruction of experiment will be caused the problem of radiating.Therefore, the present invention the 6th
Embodiment is as shown in figure 11, in light source assembly 135Part do further improvement, by light source component 131 therein5Light is removed to hide
Cover chamber 145, it is arranged at away from pedestal 115Place, then it is interpreted as by one the photocon 133 of optical fiber5By light source component 1315Excite
Light beam guide to pedestal 115On predetermined observation position.The light source component 131 located at outside after improvement5Radiating is preferable, again
Can be away from pedestal 115And then influence of the reduction to fluorescence thing to be observed.Certainly, being familiar with this technology person can deduce easily, with it
His guide structure is combined with multi-disc lens reflecting, be may achieve similar effect and should be contained in the present invention in the lump and disclose scope.
Seventh embodiment of the invention further improves the pedestal of microoptic capture device, and pedestal, which is removed, has support installation aobvious
Outside the function of micro-optics capture device body, the also function with fluorescence accessory module, as shown in figure 12, the pedestal in this example,
Except with the frame body 10 to support, install6In addition, separately in frame body 106On be provided with pedestal 116And cladding pedestal 116
Shade 126, a light masking chamber 14 is formed after both clamping connections6Space, use the interference for blocking ambient light.Shade 126Top
The object lens that portion is also formed with an offer microoptic capture device body use the perforation 121 for obtaining optical image6, and arrange in pairs or groups
There is optical filter 176, to screen out by light source reflects or diffusing reflection and Lai wavelength components, and allow the specific fluorescent of requirement of experiment
Wavelength components are largely penetrated, and to lift signal noise ratio, make experimenter be easier to obtain required information.
Light source assembly 13 in this example6, including it is arranged at shade 126On to excite the light source component 131 of fluorescence6, such as
LED etc.；And an adjustment light source component 1316Position, adjusts most suitable angle to facilitate user, collects the regulating part of optical position
1326.Certainly, also visual situation is arranged at pedestal or is respectively arranged on pedestal and shade above-mentioned light source assembly.
The pedestal of pedestal is as shown in figure 13, corresponding two breach 156Between, the guidance part 16 with a conduit path6,
Purpose is that carrier of the guiding equipped with observation thing is smoothly embedded in.Guidance part 166It is from breach 156Single port extend to the other end
Mouthful, guidance part 166Then it is located at pedestal 116Plane on.Collecting optical opticas component 18 in this example6Two lens keep certain distance, if
It is placed in pedestal 116Surface, guidance part 16 is also formed while light harvesting function is provided6Slide guide track.Collecting optical opticas component 186
One of purpose sent for convergence LED be slightly in dispersion angle excitation beam, make excitation source luminous beam by dissipating
Converging angular is accurately dispersed evenly to the region of observation to parallel.Certainly in response to the demand used, collecting optical opticas are not limited to
The integration design of component and guidance part, guidance part can also be formed directly into the bottom of pedestal, light harvesting light by being familiar with this technology person
Learn the slide guide track both sides that component is then molded over pedestal.And breach also can elasticity be designed at covering, one of pedestal；Covering, pedestal
Half and half, combination is begun to be one；Or it is formed at the bottom of pedestal.
Another aspect, because optical filter can not only filter out the wavelength components from light source component, for originally faint glimmering
Light information also can significantly weaken, thus such as inventor in No. 201210483558.0 invention patent application case institute
State, can also use halo component instead as time difference type filter, eighth embodiment of the invention is one to have as shown in Figure 14,15
The pedestal of halo component, halo component 8 therein7Plural printing opacity hole 810 is formed with one7Halo body 817, and
By belt 837One end surround, the other end of belt is then around a motor 827, when motor 827Can drive belt during operating
837, thus drive halo body 817It is rotated up to 1000 turns per second.
The purpose of this design is to exclude the interference of reflected light, allows instrument smoothly to catch the fluorescence needed for experiment.Due to fluorescence
For luminescence generated by light, luminous energy can be discharged after incident light is absorbed so that fluorescence can still continue hair after incident light disappearance
Light about 10-5Second, i.e., about 10ns.Therefore can be by above-mentioned halo component 87, with different hole arrange in pairs or groups respectively instrument camera lens with
Light source component, light source component reaches printing opacity hole 8107With printing opacity hole 8107Between when, instrument camera lens will be in face of another is saturating
Unthreaded hole gap 8107；On the contrary, light source component faces printing opacity hole 8107When, instrument camera lens is just in printing opacity hole 8107With printing opacity
Hole 8107Between.In this way, when instrument camera lens catches fluorescence information, halo body 817It will block incident light, and allow fluorescence
Appear, allow that object lens obtain this fluorescence；And during incident light beam strikes, then object lens capture is covered, in case incident light is excessively bright and reaches saturation.
Wherein, it is enhancing radiating, also can be by the light source assembly 13 of pedestal7It is respectively arranged at frame body 107With shade 127On,
To avoid light source component 1317The light source component 131 in heat influence experiment, this example is produced in energy conversion7Change located at frame sheet
Body 107On, and by a photocon 1337Such as optical fiber, by light source component 1317The luminous beam excited imports shade 127It
In.This design removes the interference of light source heat energy while light needed for providing, and lifts the stability of experiment.
In summary, the lighting angle for the light source component that low angle is excited on the one hand is adjusted by regulating part, then via collection
Light optical module makes above-mentioned excitation beam concentrate in position, and by the light of optical filter restriction specific wavelength, it is to avoid
It covers the fluorescence information to be observed, so as to obtain appropriate fluoroscopic image；Certainly, microoptic capture device not only limits to
In microscope, it might even be possible to which in application such as camera, video camera optical equipment, for example in criminal identification, related personnel can
Directly install the fluorescence accessory module of the present invention additional at the camera lens of camera, shoot the body fluid vestige picture with fluorescence protein；
Therefore, the fluoroscopic assist modular structure of the invention provided is simple, be widely used, and the quality and efficiency for sacrificing fluorescence microscopy are not had
The problem of, it is able to obtain the micro- information of height of fluorescence thing to be observed.
Only as described above, only presently preferred embodiments of the present invention, it is impossible to which the scope that the present invention is implemented is limited with this,
Every simple equivalent changes and modifications made according to claims of the present invention and description, all should still belong to of the invention special
In the range of profit covers.
Claims (19)
1. a kind of microoptic capture device fluorescence accessory module, is to be carried on one for the observation of foregoing microoptic capture device
Fluorescence thing to be observed on carrier, and at least one predetermined supporting region, the fluorescence accessory module bag are formed with the carrier
Include：
One be formed with a predetermined observation position pedestal；
One is made with alternatively non-transparent material, corresponds to link and cover said base and supply to surround out one jointly with said base
Light covers the shade of chamber, effectively to completely cut off extraneous ambient light；And
One light source assembly, including plural light source component, the direction of said light source element and foregoing predetermined observation position line, phase
It is less than 45 degree for the angle between foregoing pedestal bearing of trend, to provide low angle exciting light；
Wherein, at least one of the shade and the pedestal are formed with least one and pass in and out above-mentioned light masking chamber for above-mentioned carrier
Breach and a guidance part, the guidance part is extended along predetermined direction, for guiding above-mentioned carrier along the predetermined direction
It is mobile；And the light emission direction of said light source element is to concentrate the above-mentioned predetermined observation position towards said base.
2. fluorescence accessory module as claimed in claim 1, wherein above-mentioned breach is two breach away from each other.
3. fluorescence accessory module as claimed in claim 2, wherein above-mentioned guidance part is one group of line side along above-mentioned breach
To elongation moulding in the slide guide track slid on the pedestal, for the carrier.
4. fluorescence accessory module as claimed in claim 1 or 2, further includes one group of excitation beam direction for supplying above-mentioned light source component
The convergent collecting optical opticas component of the pedestal.
5. fluorescence accessory module as claimed in claim 1 or 2, is used to wherein foregoing microoptic capture device further includes one group
Receive the object lens that are fluoresced from above-mentioned fluorescence thing to be observed, and the shade wearing of being formed with that the foregoing object lens of confession pass
Hole, the fluorescence accessory module further includes one group of optical filter for covering above-mentioned object lens.
6. fluorescence accessory module as claimed in claim 1 or 2, wherein above-mentioned light source component is that plural rising angle is adjustable
Light emitting diode.
7. fluorescence accessory module as claimed in claim 6, wherein above-mentioned light emitting diode is disposed on the shade.
8. fluorescence accessory module as claimed in claim 6, wherein above-mentioned light emitting diode is disposed on the pedestal.
9. fluorescence accessory module as claimed in claim 6, the wherein light source assembly further include one group for adjusting above-mentioned luminous two
The regulating part of pole pipe position.
10. fluorescence accessory module as claimed in claim 9, the wherein light source assembly further include one group and are arranged at the screening for regulation
The above-mentioned light emitting diode displacement of cover, and make the regulating part of above-mentioned light emitting diode change lighting angle.
11. fluorescence accessory module as claimed in claim 9, the wherein light source assembly further include one group and are arranged at the base for regulation
The above-mentioned light emitting diode of seat, makes above-mentioned light emitting diode with respect to the regulating part of the pedestal displacement.
12. fluorescence accessory module as claimed in claim 1 or 2, wherein above-mentioned light source component is arranged on away from above-mentioned base
The position of seat, and above-mentioned light source assembly further include the luminous beam of above-mentioned light source component institute being directed to the above-mentioned predetermined observation bit of irradiation
The photocon put.
13. a kind of microoptic capture device pedestal, for one microoptic capture device body of carrying, observation is carried on one
Fluorescence thing to be observed on carrier, and at least one predetermined supporting region is formed with the carrier；The pedestal includes：
One be formed with a predetermined observation position frame body, this body further includes a pedestal；
One is made with alternatively non-transparent material, corresponds to link and cover said base and supply to surround out one jointly with said base
Light covers the shade of chamber, effectively to completely cut off extraneous ambient light；And
One light source assembly, including plural light source component, the direction of said light source element and foregoing predetermined observation position line, phase
It is less than 45 degree for the angle between foregoing pedestal bearing of trend, to provide low angle exciting light；
Wherein, at least one of the shade and the pedestal are formed with least one and pass in and out above-mentioned light masking chamber for above-mentioned carrier
Breach and a guidance part, the guidance part is extended along predetermined direction, for guiding above-mentioned carrier along the predetermined direction
It is mobile；And the light emission direction of said light source element is to concentrate the above-mentioned predetermined observation position towards said base.
14. pedestal as claimed in claim 13, extends wherein above-mentioned guidance part is one group of line direction along above-mentioned breach
The slide guide track for taking shape on the pedestal, being slid for the carrier.
15. pedestal as claimed in claim 13, further includes one group of excitation beam for above-mentioned light source component and is received towards the pedestal
The collecting optical opticas component held back；And, wherein foregoing microoptic capture device further includes one group comes from above-mentioned fluorescence to receive
The object lens that thing to be observed is fluoresced, and the shade is formed with the perforation that the foregoing object lens of confession are passed, the fluorescence accessory module
Further include one group of optical filter for covering above-mentioned object lens.
16. pedestal as claimed in claim 13, wherein above-mentioned light source component is the adjustable light-emitting diodes of plural rising angle
Pipe.
17. pedestal as claimed in claim 16, the wherein light source assembly further include one group for adjusting above-mentioned light emitting diode position
The regulating part put.
18. pedestal as claimed in claim 13, wherein above-mentioned light source component is to be arranged on the position away from said base, with
And above-mentioned light source assembly further include by above-mentioned light source component luminous beam be directed to irradiate above-mentioned predetermined observation position light guide member
Part.
19. a kind of microoptic capture device, including：
One fuselage and the object lens for being installed in the fuselage；And
One is arranged at the fluorescence accessory module of the fuselage, and the fluorescence accessory module is held for the observation of foregoing microoptic capture device
The fluorescence thing to be observed being loaded on a carrier, and at least one predetermined supporting region is formed with the carrier, including：
One be formed with a predetermined observation position pedestal；
One is made with alternatively non-transparent material, corresponds to link and cover said base and supply to surround out one jointly with said base
Light covers the shade of chamber, effectively to completely cut off extraneous ambient light；And
One light source assembly, including plural number are arranged at the light source component that the low angle of the one of the shade and the pedestal is excited, preceding
The direction of light source component and foregoing predetermined observation position line is stated, is less than 45 relative to the angle between foregoing pedestal bearing of trend
Degree, to provide low angle exciting light；
Wherein, at least one of the shade and the pedestal are formed with least one and pass in and out above-mentioned light masking chamber for above-mentioned carrier
Breach and a guidance part, the guidance part is extended along predetermined direction, for guiding above-mentioned carrier along the predetermined direction
It is mobile；And the light emission direction of said light source element is to concentrate the above-mentioned predetermined observation position towards said base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310047142.9A CN103777336B (en) | 2012-10-22 | 2013-02-06 | Microoptic capture device fluorescence accessory module, pedestal and the device |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104029315 | 2012-10-22 | ||
| CN201210402931.5 | 2012-10-22 | ||
| CN201210402931 | 2012-10-22 | ||
| CN201310047142.9A CN103777336B (en) | 2012-10-22 | 2013-02-06 | Microoptic capture device fluorescence accessory module, pedestal and the device |
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| Publication Number | Publication Date |
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| CN103777336A CN103777336A (en) | 2014-05-07 |
| CN103777336B true CN103777336B (en) | 2017-09-05 |
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| CN201310047142.9A Expired - Fee Related CN103777336B (en) | 2012-10-22 | 2013-02-06 | Microoptic capture device fluorescence accessory module, pedestal and the device |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105486667A (en) * | 2015-07-01 | 2016-04-13 | 上海睿钰生物科技有限公司 | Integrated fluorescence excitation light source apparatus |
| CN110568601A (en) * | 2019-08-30 | 2019-12-13 | 北京临近空间飞行器系统工程研究所 | Image scanning system |
| CN110632750B (en) * | 2019-08-30 | 2024-01-12 | 北京临近空间飞行器系统工程研究所 | Fluorescent microscopic optical system and fluorescent staining cell scanning and analyzing system |
| CN111239086B (en) * | 2019-08-30 | 2024-04-05 | 北京临近空间飞行器系统工程研究所 | Visual background device, fluorescence microscopic optical system and scanning analysis system |
| CN110514633A (en) * | 2019-08-30 | 2019-11-29 | 北京临近空间飞行器系统工程研究所 | Light supply apparatus, fluorescence microscopy optical system, scanning and analysis system |
| CN114287881B (en) * | 2021-12-11 | 2024-03-15 | 中国科学院深圳先进技术研究院 | Miniature single photon fluorescence microscope implantation device and implantation method |
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| CN103777336A (en) | 2014-05-07 |
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