CN110514633A - Light supply apparatus, fluorescence microscopy optical system, scanning and analysis system - Google Patents
Light supply apparatus, fluorescence microscopy optical system, scanning and analysis system Download PDFInfo
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- CN110514633A CN110514633A CN201910817728.6A CN201910817728A CN110514633A CN 110514633 A CN110514633 A CN 110514633A CN 201910817728 A CN201910817728 A CN 201910817728A CN 110514633 A CN110514633 A CN 110514633A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 54
- 238000000799 fluorescence microscopy Methods 0.000 title claims abstract description 45
- 238000004458 analytical method Methods 0.000 title claims abstract description 26
- 239000011324 bead Substances 0.000 claims abstract description 36
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 23
- 230000000007 visual effect Effects 0.000 claims description 46
- 239000000758 substrate Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 7
- 230000035945 sensitivity Effects 0.000 claims description 4
- 239000004744 fabric Substances 0.000 claims description 2
- 230000003447 ipsilateral effect Effects 0.000 claims description 2
- 230000004044 response Effects 0.000 claims description 2
- 208000033748 Device issues Diseases 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 7
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 238000009795 derivation Methods 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 239000011796 hollow space material Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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Abstract
A kind of LED light source device, fluorescence microscopy optical system and fluorescent staining cellscan and analysis system are provided in the embodiment of the present application.LED light source device includes: convex lens, and the front arc of convex lens is spherical surface；At least two LED light source mould groups, LED light source mould group and the front arc of the convex lens are oppositely arranged, and the center of the lamp bead of each LED light source mould group is respectively facing the centre of sphere of the front arc of the convex lens；The centre of sphere convergence of front arc of the light that the LED light source mould group issues through the convex lens to the convex lens.Fluorescence microscopy optical system includes above-mentioned LED light source device.Fluorescent staining cellscan and analysis system include above-mentioned LED light source device.Fluorescent staining cellscan and analysis system include fluorescence microscopy optical system.The embodiment of the present application solves the technical issues of luminance shortage of traditional LED light source device of fluorescent staining cellscan and analysis system.
Description
Technical field
This application involves LED light source technical fields, and in particular, to a kind of LED light source device, fluorescence microscopy optical system
With fluorescent staining cellscan and analysis system.
Background technique
Traditional LED light source device in fluorescent staining cellscan and analysis system, as shown in Figure 1, including convex lens 10
The LED light source mould group 20 being arranged in contrast.Since structure limits, the lamp bead 21 of LED light source mould group only one being capable of cloth
The focal point in convex lens is set, thus leads to the luminance shortage of LED light source device, hot spot uniformity is also insufficient.
Therefore, the luminance shortage of LED light source device is that those skilled in the art are badly in need of technical problems to be solved.
Disclosed above- mentioned information are only used for reinforcing the understanding to the background of the application in the background technology, therefore it may be wrapped
Containing the information for not being formed as the prior art that those of ordinary skill in the art are known.
Summary of the invention
A kind of LED light source device is provided in the embodiment of the present application, fluorescence microscopy optical system and fluorescent staining cell are swept
It retouches and analysis system, the technical issues of luminance shortage to solve traditional LED light source device.
The embodiment of the present application provides a kind of LED light source device, is used for fluorescent staining cellscan and analysis system, packet
It includes:
Convex lens, the front arc of the convex lens are spherical surface；
At least two LED light source mould groups, the LED light source mould group and the front arc of the convex lens are oppositely arranged, and each
The center of the lamp bead of a LED light source mould group is respectively facing the centre of sphere of the front arc of the convex lens；
Wherein, the centre of sphere of front arc of the light that the LED light source mould group issues through the convex lens to the convex lens converges
It is poly-.
The embodiment of the present application also provides a kind of fluorescence microscopy optical systems, for fluorescent staining cellscan and analysis system
System, including above-mentioned LED light source device.
The embodiment of the present application also provides a kind of fluorescent staining cellscan and analysis systems, including above-mentioned LED light source to fill
It sets.
The embodiment of the present application also provides a kind of fluorescent staining cellscan and analysis systems, including above-mentioned fluorescence microscopy light
System.
The embodiment of the present application due to using the technology described above, has following technical effect that
LED light source mould group and the front arc of the convex lens are oppositely arranged, and the lamp bead of each LED light source mould group
Center is respectively facing the centre of sphere of the front arc of the convex lens.Compared with LED light source device traditional in background technique, the application
The LED light source device of embodiment, firstly, LED light source mould group is more, secondly, to the position of LED light source mould group setting into
Capable limitation, the center of the lamp bead of each LED light source mould group is respectively facing the centre of sphere of the front arc of the convex lens, in this way, respectively
The centre of sphere convergence of front arc of the light that the lamp bead of a LED light source mould group issues to the convex lens, thus before the convex lens
Brightness around the centre of sphere of cambered surface is higher, simultaneously as being each LED light source mould around the centre of sphere of the front arc of the convex lens
The position of the light interaction compensation of group, therefore, the uniformity of the hot spot around the centre of sphere of the front arc of the convex lens is higher.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present application, constitutes part of this application, this Shen
Illustrative embodiments and their description please are not constituted an undue limitation on the present application for explaining the application.In the accompanying drawings:
Fig. 1 is the schematic diagram of traditional LED light source device；
Fig. 2 is a kind of schematic diagram of LED light source device of the embodiment of the present application；
Fig. 3 is the LED light source mould group of LED light source device shown in Fig. 2 and the schematic diagram that fixed plate is fixed；
Fig. 4 is the geometrical relationship schematic diagram of LED light source device shown in Fig. 2；
Fig. 5 is the schematic diagram of the fluorescence microscopy optical system of the embodiment of the present application；
Fig. 6 is the schematic diagram of the visual background device of Fig. 5 fluorescence microscopy optical system；
Fig. 7 is the schematic diagram of visual background device and object lens shown in fig. 6.
Description of symbols:
In background technique:
10 convex lenses, 20LED light source module group, the lamp bead of 21LED light source module group；
In the embodiment of the present application:
100LED light supply apparatus,
100-1 convex lens, the front arc of 110 convex lenses, the centre of sphere of the front arc of 120 convex lenses,
The primary optical axis of 130 convex lenses,
140LED light source module group, 141 lamp beads, 142 substrates, 150 fixed plates,
310 non-reflecting regions, 320 fluorescent plates, 321 current supply lines, 331 object lens, 332 are observed object,
333PWM dimming device, 334 filtering apparatus.
Specific embodiment
In order to which technical solution in the embodiment of the present application and advantage is more clearly understood, below in conjunction with attached drawing to the application
Exemplary embodiment be described in more detail, it is clear that described embodiment be only the application a part implement
Example, rather than the exhaustion of all embodiments.It should be noted that in the absence of conflict, embodiment and reality in the application
The feature applied in example can be combined with each other.
Fluorescent staining cellscan and analysis system, abbreviation CTC scanning and analysis system, are adhered to on needle-shaped carrier
The system that staining cell carries out 360 degree of image scans and identification.Fluorescent staining cellscan and analysis system include multiple hardware
Equipment, software is equipped with analysis software.LED light source device in the following examples one is fluorescent staining cellscan and analysis
The LED light source device of system, the fluorescence microscopy optical system in embodiment two is fluorescent staining cellscan and analysis system
Fluorescence microscopy optical system.
Embodiment one
Fig. 2 is a kind of schematic diagram of LED light source device of the embodiment of the present application；Fig. 3 is LED light source device shown in Fig. 2
LED light source mould group and the fixed schematic diagram of fixed plate.
As shown in Figures 2 and 3, the LED light source device 100 of the embodiment of the present application for fluorescent staining cellscan and is divided
Analysis system, comprising:
Convex lens 100-1, the front arc 110 of the convex lens are spherical surface；
At least two LED light source mould groups 140, the LED light source mould group 140 are opposite with the front arc 110 of the convex lens
Setting, and the center of the lamp bead 141 of each LED light source mould group is respectively facing the centre of sphere 120 of the front arc of the convex lens；
Wherein, the light that the LED light source mould group 140 issues is through the convex lens 100-1 to the front arc of the convex lens
The centre of sphere 120 converge.
The LED light source device of the embodiment of the present application, LED light source mould group and the front arc of the convex lens are oppositely arranged, and
The center of the lamp bead of each LED light source mould group is respectively facing the centre of sphere of the front arc of the convex lens.In background technique
Traditional LED light source device is compared, the LED light source device of the embodiment of the present application, firstly, LED light source mould group is more,
Secondary, to the limitation that the position of LED light source mould group setting carries out, the center of the lamp bead of each LED light source mould group is respectively facing described
The centre of sphere of the front arc of convex lens, in this way, the front arc of the lamp bead of each LED light source mould group light that issues to the convex lens
Centre of sphere convergence, so that the brightness around the centre of sphere of the front arc of the convex lens is higher, simultaneously as the frontal arc of the convex lens
It is the position of the light interaction compensation of each LED light source mould group, therefore, the centre of sphere of the front arc of the convex lens around the centre of sphere in face
The uniformity of the hot spot of surrounding is higher.
In implementation, as shown in Fig. 2, the lamp bead of one of LED light source mould group is located at the focal point of the convex lens, for coke
Point LED light source mould group；
The centre of sphere 120 of the front arc of the convex lens is located on the primary optical axis 130 of the convex lens.
Focus LED light source mould group is located at the focal point of convex lens, and the convergence of the light of convex lens focus point LED light source mould group is made
With preferable, so that the luminous intensity around the centre of sphere of the front arc of the convex lens is higher.
In implementation, as shown in Fig. 2, the LED light source mould group in addition to the focus LED light source mould group is side LED light source
Mould group；
The lamp bead of the side LED light source mould group is tilted to the key light axis direction of the convex lens, to realize the side
The center of the lamp bead of LED light source mould group towards the convex lens front arc the centre of sphere.
Using the above structure, it can easily realize the center of the lamp bead of side LED light source mould group towards the convex lens
Front arc the centre of sphere.
In implementation, as shown in Fig. 2, the projection of primary optical axis of the lamp bead of the side LED light source mould group in the convex lens
Between the lamp bead and the convex lens of the focus LED light source mould group.
That is focal length of the object distance of the lamp bead of side LED light source mould group less than convex lens, the lamp bead hair of side LED light source mould group
The light that the lamp bead of hot spot and focus LED light source mould group that light out is formed around the centre of sphere of the front arc of the convex lens issues
The hot spot formed around the centre of sphere of the front arc of the convex lens staggeredly compensates preferably, around the centre of sphere of the front arc of convex lens
Spot intensity it is higher, uniformity is also higher.
In implementation, the side LED light source mould group is n, and n is greater than the integer equal to 2；
N side LED light source mould groups be using the focus LED light source mould group as the center of circle same circle even circumferential
Distribution.
Even circumferential of the side LED light source mould group using the focus LED light source mould group as the center of circle in same circle is distributed, then
The hot spot formed around the centre of sphere of the front arc of convex lens is also substantially at circle.
In implementation, as an alternative embodiment, as shown in Figures 2 and 3, the side LED light source mould group is two
It is a；
Two side LED light source mould groups are symmetrical arranged relative to the focus LED light source mould group.
Focus LED light source mould group and two side LED light source modular arrangements are at linear, then in the front arc of convex lens
The hot spot formed around the centre of sphere is also substantially at linear.
In implementation, as shown in Figures 2 and 3, have between the focus LED light source mould group and the side LED light source mould group
There is interval.
Spaced LED light source mould group, is conducive to the heat dissipation of LED light source mould group.
In implementation, as shown in Fig. 2, LED light source mould group further includes fixed plate 150, for fixing the LED light source mould group
140；
The fixed plate 150 and the front arc 110 of the convex lens are oppositely arranged, and the focus LED light source mould group is fixed
In the center of the inner plate surface of the fixed plate.
Fixed plate realizes the fixation of multiple LED light source mould groups, and the focus LED light source mould group is fixed on the fixed plate
The center of inner plate surface, the location rule of setting, manufacture easy to process.
In implementation, as shown in Fig. 2, primary optical axis 130 of the marginal position of the inner plate surface of the fixed plate to the convex lens
Direction inclination；
The side LED light source mould group is fixed on the marginal position of the inner plate surface of the fixed plate, to realize the side
The lamp bead of LED light source mould group is tilted to 130 direction of primary optical axis of the convex lens；
Wherein, the fixed plate and the LED light source mould group form LED light source component.
It is tilted by the marginal position of the inner plate surface of fixed plate to the key light axis direction of the convex lens, realizes side LED
The lamp bead of light source module group is tilted to the key light axis direction of the convex lens, and structure is simple, is easy to implement.
In implementation, as shown in figure 3, the LED light source mould group 140 includes the substrate 142 of square and is fixed on the base
The lamp bead 141 of plate center；Substrate can be square as shown in Figure 3, be also possible to other shapes, such as round, rectangular
Shape etc.；
The substrate is fixed with the fixed plate, to realize the fixation of the LED light source mould group and the fixed plate.
In implementation, meet following relationship between the LED light source mould group and the convex lens:
Wherein, b is the center of the lamp bead of the side LED light source mould group and the lamp bead of the focus LED light source mould group
Center between the projection in the perpendicular direction of the primary optical axis of the convex lens at a distance from；
φ is the diameter of the convex lens, and D is the focal length of convex lens,
L is the side length of the substrate of LED light source mould group,
θ is key light axis direction inclined angle of the side LED light source mould group relative to the convex lens,
α be the side LED light source mould group lamp bead center to the convex lens ipsilateral penumbra angle.
Derivation process it is as follows:
The marginal position of the inner plate surface of the fixed plate is equal to institute to the inclined angle of key light axis direction of the convex lens
State key light axis direction inclined angle and θ of the side LED light source mould group relative to the convex lens.Fig. 4 is shown in Fig. 2
The geometrical relationship schematic diagram of LED light source device, as shown in figure 4, in △ ABC, according to geometrical relationship, angle BAC=α-θ；Then
Due toBC and AB are brought intoIt can derive
Out
In implementation, b also meets following relationship:
Derivation process it is as follows:
As shown in figure 4, according to geometrical relationship,Due toIt can derive
In implementation, θ also meets following relationship:
Wherein, r is the radius of ball where the front arc of the convex lens.
Derivation process it is as follows: as shown in figure 4, in △ UVW, according to geometrical relationship,It can derive
In implementation, θ and b should meet relational expressionWithUnder conditions of take minimum
Value.
Embodiment two
Fig. 5 is the schematic diagram of the fluorescence microscopy optical system of the embodiment of the present application, and Fig. 6 is Fig. 5 fluorescence microscopy optical system
Visual background device schematic diagram；Fig. 7 is the schematic diagram of visual background device and object lens shown in fig. 6.
As shown in figure 5, the embodiment of the present application provides a kind of fluorescence microscopy optical system, for fluorescent staining cellscan and
Analysis system.LED light source device 100 including embodiment one, LED light source device are a part in PWM dimming device 333.
In implementation, as shown in figure 5, fluorescence microscopy optical system further includes visual background device, object lens 331, filtering apparatus
334。
As shown in Figure 6 and Figure 7, the visual background device of the fluorescence microscopy optical system of the embodiment of the present application, the vision
The wherein side of Background apparatus is capable of providing fluorescence as the bias light for being observed object 332, and the visual background device can
The side for providing fluorescence has non-reflecting regions 310, and the non-reflecting regions 310 carry out across or absorb to exciting light；
Wherein, the non-reflecting regions 310 reduce the vision back towards the object lens of the fluorescence microscopy optical system
Reflection of the scape device to exciting light, the exciting light are the exciting lights through the object lens of the fluorescence microscopy optical system.
The visual background device be capable of providing fluorescence side have non-reflecting regions, non-reflecting regions to exciting light not
It is reflected, but exciting light is carried out across or absorbed.In this way, due to the presence of non-reflecting regions, visual background device pair
The reflection of exciting light is not present or less.Compared with the background art, visual background device is less to the reflection of exciting light, reflective existing
As smaller, fluorescence microscopy optical system will not generate halation being observed object surface in micro-imaging, improve fluorescence microscopy
The quality of imaging.
In implementation, as shown in Figure 6 and Figure 7, the non-reflecting regions 310 are through visual background device thickness direction
Hollow region.
In this way, hollow region is as non-reflecting regions, exciting light can be directly through；Meanwhile visual background device at
This is relatively low.
In implementation, the size that the visual background device provides the outer profile of the side of fluorescence is greater than the visual field of the object lens
Diameter.
In this way, the visual background device can provide fluorescence for the field range of entire object lens, the visual field of object lens is improved
Brightness.
In implementation, the visual background device can be the visual background device of annular or rectangle frame.
In this way, the visual background device of annular or rectangle frame, hollow space is as non-reflecting regions, the side of entity part
It is capable of providing fluorescence, the fluorescence of the field range of entire object lens is more uniform.
In implementation, as a kind of optional mode, as shown in Figure 6 and Figure 7, the visual background device includes:
Two symmetrically arranged fluorescent plates 320, the wherein side of the fluorescent plate are capable of providing fluorescence, each fluorescence
The emission side of plate towards the same side, and between two fluorescent plates interval setting using as the hollow of the visual background device
Region.
The visual background device of above structure, structure is simple, manufacture easy to process.
In implementation, the fluorescent plate is the fluorescent plate of monochromatic source, and each fluorescent plate passes through current supply line 321 and electricity
Way switch connects to power supply；
Wherein, the circuit switch is used to control the on/off of the fluorescent plate, to control the visual background device
The presence or absence of fluorescence.
Fluorescent plate is active fluorescent plate, firstly, the intensity of the fluorescence issued is relatively stable, fluorescence microscopy optical system exists
Imaging effect also can be relatively stable when micro-imaging；Secondly, the presence or absence of the fluorescence of visual background device can flexibly be controlled, more
Add flexibly；Again, fluorescent plate provides the wavelength of fluorescence and intensity can flexible choice according to actual needs.
In implementation, as shown in Figure 6 and Figure 7, the fluorescent plate 320 is the fluorescent plate of rectangle.
The fluorescent plate simple shape of rectangle, manufacture easy to process.
In implementation, as shown in fig. 7, the width of hollow region meets following relationship between two fluorescent plates 320:
2 × s of a > × tan β；
Wherein, a is the width of hollow region between two fluorescent plates, and s is that the object lens and the visual background fill
The distance between set, β is the angle of divergence through the exciting light of the object lens.
As shown in fig. 7, s=p+q, p are the distances for being observed object 332 and arriving object lens, q is to be observed object 332 to the vision
Background apparatus is capable of providing the distance of the side of fluorescence；Or p is at a distance from marker to object lens, q is marker described in
Visual background device is capable of providing the distance of the side of fluorescence, and marker is fixed with the distance between object is observed.
β is the angle of divergence through the exciting light of the object lens, is after the frequency of object lens and exciting light determines, the value of β is also
It has determined.2 × s of a > × tan β derivation process is as follows:
As shown in fig. 7, in △ XYZ, according to geometrical relationship,
Due to YZ=s,It can derive 2 × s of a > × tan β.
In implementation, the length C of the fluorescent plate1Greater than the diameter in the object lens visual field, two fluorescent plate long sides
The distance between outer edge is greater than the diameter in the object lens visual field.
The distance between the outer edge of the length of fluorescent plate and two fluorescent plate long sides is all greater than the object lens visual field
The fluorescence of diameter, the field range of entire object lens is more uniform.
As an optional mode, the length C of the fluorescent plate1It is 1 millimeter bigger than the diameter in the object lens visual field.
As an optional mode, the width C of the fluorescent plate2More than or equal to 0.1 millimeter.
In implementation, the fluorescent plate, the filtering apparatus and fluorescence camera of the fluorescence microscopy optical system meets with ShiShimonoseki
It is formula:
ε < λ (f0)×E0< K；
Wherein, f0For the frequency for the fluorescence that the fluorescent plate provides, E0It is f for frequency0Fluorescence energy, λ (f0) for institute
The filtering apparatus for stating fluorescence microscopy optical system is f to frequency0Fluorescence response rate, ε be the fluorescence microscopy optical system
Fluorescence camera minimum sensitivity, K be the fluorescence microscopy optical system fluorescence camera sensitivity maximum.
λ(f0)×E0It is exactly the energy of fluorescence, ε < λ (f0)×E0< K is exactly to express the energy of fluorescence in fluorescence camera
In sensitive volume.
Embodiment three
A kind of fluorescent staining cellscan and analysis system of the embodiment of the present application, including LED light described in embodiment one
Source device.
Example IV
A kind of fluorescent staining cellscan and analysis system of the embodiment of the present application, it is aobvious including fluorescence described in embodiment two
Micro-optical systems.
In the description of the application and embodiment, it is to be understood that the instructions such as term "top", "bottom", " height "
Orientation or positional relationship is to be based on the orientation or positional relationship shown in the drawings, and is merely for convenience of description the application and simplification is retouched
It states, rather than the device or element of indication or suggestion meaning must have a particular orientation, be constructed and operated in a specific orientation,
Therefore it should not be understood as the limitation to the application.
In the application and embodiment unless specifically defined or limited otherwise, term " setting ", " installation ", " phase
Even ", the terms such as " connection ", " fixation " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or
Integrally；It can be mechanical connection, be also possible to be electrically connected, can also be communication；It can be directly connected, it can also be in
Between medium be indirectly connected, can be the connection inside two elements or the interaction relationship of two elements.For this field
For those of ordinary skill, the concrete meaning of above-mentioned term in this application can be understood as the case may be.
In the application and embodiment unless specifically defined or limited otherwise, fisrt feature second feature it
"upper" or "lower" may include that the first and second features directly contact, may include the first and second features be not directly to connect yet
It touches but by the other characterisation contact between them.Moreover, fisrt feature second feature " on ", " top " and " on
Face " includes fisrt feature right above second feature and oblique upper, or to be merely representative of first feature horizontal height special higher than second
Sign.Fisrt feature under the second feature " below ", " below " and " below " include fisrt feature right above second feature and tiltedly on
Side, or first feature horizontal height is merely representative of less than second feature.
Above disclosure provides many different embodiments or example is used to realize the different structure of the application.In order to
Simplify disclosure herein, above the component of specific examples and setting are described.Certainly, they are merely examples, and
And purpose does not lie in limitation the application.In addition, the application can in different examples repeat reference numerals and/or reference letter,
This repetition is for purposes of simplicity and clarity, itself not indicate between discussed various embodiments and/or setting
Relationship.In addition, this application provides various specific techniques and material example, but those of ordinary skill in the art can be with
Recognize the application of other techniques and/or the use of other materials.
Although the preferred embodiment of the application has been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the application range.
Obviously, those skilled in the art can carry out various modification and variations without departing from the essence of the application to the application
Mind and range.In this way, if these modifications and variations of the application belong to the range of the claim of this application and its equivalent technologies
Within, then the application is also intended to include these modifications and variations.
Claims (29)
1. a kind of LED light source device is used for fluorescent staining cellscan and analysis system characterized by comprising
Convex lens, the front arc of the convex lens are spherical surface；
At least two LED light source mould groups, the LED light source mould group and the front arc of the convex lens are oppositely arranged, and each institute
State the lamp bead of LED light source mould group center be respectively facing the convex lens front arc the centre of sphere；
Wherein, the centre of sphere convergence of front arc of the light that the LED light source mould group issues through the convex lens to the convex lens.
2. LED light source device according to claim 1, which is characterized in that the lamp bead of one of LED light source mould group is located at
The focal point of the convex lens is focus LED light source mould group；
The centre of sphere of the front arc of the convex lens is located on the primary optical axis of the convex lens.
3. LED light source device according to claim 2, which is characterized in that in addition to the focus LED light source mould group
LED light source mould group is side LED light source mould group；
The lamp bead of the side LED light source mould group is tilted to the key light axis direction of the convex lens, to realize the side LED light
The center of the lamp bead of source mould group towards the convex lens front arc the centre of sphere.
4. LED light source device according to claim 3, which is characterized in that the lamp bead of the side LED light source mould group is in institute
The projection for stating the primary optical axis of convex lens is located between the lamp bead and the convex lens of the focus LED light source mould group.
5. LED light source device according to claim 4, which is characterized in that the side LED light source mould group is n, and n is
Integer more than or equal to 2；
The n side LED light source mould groups are that the even circumferential using the focus LED light source mould group as the center of circle in same circle divides
Cloth.
6. LED light source device according to claim 4, which is characterized in that the side LED light source mould group is two；
Two side LED light source mould groups are symmetrical arranged relative to the focus LED light source mould group.
7. LED light source device according to claim 6, which is characterized in that the focus LED light source mould group and the side
There is interval between LED light source mould group.
8. LED light source device according to claim 7, which is characterized in that further include fixed plate, for fixing the LED
Light source module group；
The fixed plate and the front arc of the convex lens are oppositely arranged, and the focus LED light source mould group is fixed on the fixation
The center of the inner plate surface of plate.
9. LED light source device according to claim 8, which is characterized in that the marginal position of the inner plate surface of the fixed plate
It is tilted to the key light axis direction of the convex lens；
The side LED light source mould group is fixed on the marginal position of the inner plate surface of the fixed plate, to realize the side LED light
The lamp bead of source mould group is tilted to the key light axis direction of the convex lens.
10. LED light source device according to claim 9, which is characterized in that the LED light source mould group includes square
Substrate and the lamp bead for being fixed on the substrate center position；
The substrate is fixed with the fixed plate, to realize the fixation of the LED light source mould group and the fixed plate.
11. LED light source device according to claim 10, which is characterized in that the LED light source mould group and the convex lens
Between meet following relationship:
Wherein, b is the center at the center of the lamp bead of the side LED light source mould group and the lamp bead of the focus LED light source mould group
At a distance between the projection in the perpendicular direction of the primary optical axis of the convex lens；
φ is the diameter of the convex lens, and D is the focal length of convex lens,
L is the side length of the substrate of LED light source mould group,
θ is key light axis direction inclined angle of the side LED light source mould group relative to the convex lens,
α be the side LED light source mould group lamp bead center to the convex lens ipsilateral penumbra angle.
12. LED light source device according to claim 11, which is characterized in that b also meets following relationship:
13. LED light source device according to claim 12, which is characterized in that θ also meets following relationship:
Wherein, r is the radius of ball where the front arc of the convex lens.
14. LED light source device according to claim 13, which is characterized in that θ and b should meet relational expressionWithUnder conditions of be minimized.
15. a kind of fluorescence microscopy optical system is used for fluorescent staining cellscan and analysis system, which is characterized in that including upper
State any LED light source device of claim 1 to 14.
16. fluorescence microscopy optical system according to claim 15, which is characterized in that further include:
Visual background device, the wherein side of the visual background device are capable of providing fluorescence as the bias light for being observed object,
And the side that the visual background device is capable of providing fluorescence has non-reflecting regions, the non-reflecting regions carry out exciting light
It passes through or absorbs；
Wherein, the non-reflecting regions reduce the visual background device pair towards the object lens of the fluorescence microscopy optical system
The reflection of exciting light, the exciting light are optical filtering of the light through the fluorescence microscopy optical system that the LED light source device issues
It is formed after device through the object lens.
17. fluorescence microscopy optical system according to claim 16, which is characterized in that the non-reflecting regions are through institute
State the hollow region in visual background device thickness direction.
18. fluorescence microscopy optical system according to claim 17, which is characterized in that the visual background device provides glimmering
The size of the outer profile of the side of light is greater than the diameter in the visual field of the object lens.
19. fluorescence microscopy optical system according to claim 18, which is characterized in that the visual background device is annular
Or the visual background device of rectangle frame.
20. fluorescence microscopy optical system according to claim 18, which is characterized in that the visual background device includes:
Two symmetrically arranged fluorescent plates, the wherein side of the fluorescent plate are capable of providing fluorescence, the hair of each fluorescent plate
Towards the same side, and between two fluorescent plates, interval is arranged using the hollow region as the visual background device for light side.
21. fluorescence microscopy optical system according to claim 20, which is characterized in that the fluorescent plate is monochromatic source
Fluorescent plate, each fluorescent plate are connected to power supply by current supply line and circuit switch；
Wherein, the circuit switch is used to control the on/off of the fluorescent plate, to control the glimmering of the visual background device
The presence or absence of light.
22. fluorescence microscopy optical system according to claim 21, which is characterized in that the fluorescent plate is the fluorescence of rectangle
Plate.
23. fluorescence microscopy optical system according to claim 22, which is characterized in that hollow between two fluorescent plates
The width in region meets following relationship:
2 × s of a > × tan β；
Wherein, a is the width of hollow region between two fluorescent plates, s be the object lens and the visual background device it
Between distance, β be through the object lens exciting light the angle of divergence.
24. fluorescence microscopy optical system according to claim 23, which is characterized in that the length of the fluorescent plate is greater than institute
The diameter in the object lens visual field is stated, the distance between outer edge of two fluorescent plate long sides is greater than the diameter in the object lens visual field.
25. fluorescence microscopy optical system according to claim 24, which is characterized in that described in the length ratio of the fluorescent plate
The diameter in the object lens visual field is 1 millimeter big.
26. fluorescence microscopy optical system according to claim 25, which is characterized in that the width of the fluorescent plate be greater than etc.
In 0.1 millimeter.
27. fluorescence microscopy optical system according to claim 26, which is characterized in that the fluorescent plate, the fluorescence are aobvious
The filtering apparatus and fluorescence camera of micro-optical systems meet following relationship:
ε < λ (f0)×E0< K；
Wherein, f0For the frequency for the fluorescence that the fluorescent plate provides, E0It is f for frequency0Fluorescence energy, λ (f0) it is described glimmering
The filtering apparatus of light micro optical system is f to frequency0Fluorescence response rate, ε be the fluorescence microscopy optical system it is glimmering
The minimum sensitivity of light camera, K are the sensitivity maximum of the fluorescence camera of the fluorescence microscopy optical system.
28. a kind of fluorescent staining cellscan and analysis system, which is characterized in that any described including claim 1 to 14
LED light source device.
29. a kind of fluorescent staining cellscan and analysis system, which is characterized in that any described including claim 15 to 27
Fluorescence microscopy optical system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910817728.6A CN110514633A (en) | 2019-08-30 | 2019-08-30 | Light supply apparatus, fluorescence microscopy optical system, scanning and analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910817728.6A CN110514633A (en) | 2019-08-30 | 2019-08-30 | Light supply apparatus, fluorescence microscopy optical system, scanning and analysis system |
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| CN110514633A true CN110514633A (en) | 2019-11-29 |
Family
ID=68628703
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| CN201910817728.6A Pending CN110514633A (en) | 2019-08-30 | 2019-08-30 | Light supply apparatus, fluorescence microscopy optical system, scanning and analysis system |
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| CN108518590A (en) * | 2018-03-26 | 2018-09-11 | 杨毅 | Laser light source and lamps and lanterns |
| CN210834667U (en) * | 2019-08-30 | 2020-06-23 | 北京临近空间飞行器系统工程研究所 | LED light source device |
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| US20010048082A1 (en) * | 2000-01-07 | 2001-12-06 | Yuri Osipchuk | Scanning microscope |
| CN102305782A (en) * | 2011-08-10 | 2012-01-04 | 浙江大学 | Method and device for analyzing fluorescent correlation spectroscopy based on medium microsphere |
| CN103777336A (en) * | 2012-10-22 | 2014-05-07 | 承奕科技股份有限公司 | fluorescence assistive module, pedestal for microscopic optical capture device and microscopic optical capture device |
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