CN103773845A - Side flow test strip detection kit for detecting Cervusaxis in rood and feed and application thereof - Google Patents
Side flow test strip detection kit for detecting Cervusaxis in rood and feed and application thereof Download PDFInfo
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- CN103773845A CN103773845A CN201310670347.2A CN201310670347A CN103773845A CN 103773845 A CN103773845 A CN 103773845A CN 201310670347 A CN201310670347 A CN 201310670347A CN 103773845 A CN103773845 A CN 103773845A
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- nucleic acid
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-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- Bioinformatics & Cheminformatics (AREA)
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Abstract
Description
Template DNA: deer Mitochondrial DNA | 1.0 μl |
PrimerF/R | 0.8 μl |
dNTP | 2.0 μl |
10X PCRbuffer | 2.0 μl |
HS Taq DNA polymerase | 0.2 μl |
ddH2O | 13.2 μl |
Cumulative volume | 20 μl |
95 |
5 min |
94 ℃ | 30 sec |
55 ℃ | 30 sec |
72 ℃ | 30 sec |
72 |
5 min |
Totally 30 circulations | ? |
Template DNA: |
10 μl |
PrimerF/R | 0.8 μl |
dNTP | 2.0 μl |
10X PCRbuffer | 2.0 μl |
HS Taq DNA polymerase | 0.2 μl |
ddH2O | 4.2 μl |
Cumulative volume | 20 μl |
95 |
5 min |
94 ℃ | 30 sec |
57 ℃ | 30 sec |
72 ℃ | 80 sec |
72 ℃ | 5 min |
Totally 30 circulations | ? |
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310670347.2A CN103773845B (en) | 2013-12-10 | 2013-12-10 | Deer derived components detection lateral flow ELISA test strip test kit and its application in a kind of food and feedstuff |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310670347.2A CN103773845B (en) | 2013-12-10 | 2013-12-10 | Deer derived components detection lateral flow ELISA test strip test kit and its application in a kind of food and feedstuff |
Publications (2)
Publication Number | Publication Date |
---|---|
CN103773845A true CN103773845A (en) | 2014-05-07 |
CN103773845B CN103773845B (en) | 2017-03-01 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310670347.2A Expired - Fee Related CN103773845B (en) | 2013-12-10 | 2013-12-10 | Deer derived components detection lateral flow ELISA test strip test kit and its application in a kind of food and feedstuff |
Country Status (1)
Country | Link |
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CN (1) | CN103773845B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104017872A (en) * | 2014-05-29 | 2014-09-03 | 天津出入境检验检疫局动植物与食品检测中心 | Lateral flow test paper strip detection kit for detection of fox source components in food and feed and application thereof |
CN114686600A (en) * | 2022-02-24 | 2022-07-01 | 宁波大学 | Primer group and method for meat detection based on seven-fold PCR technology |
Citations (3)
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CN1811447A (en) * | 2006-02-08 | 2006-08-02 | 杭州优思达生物技术有限公司 | Nucleic acid membrane chromatographic fast detecting method and its test paper bar and use thereof |
CN102520172A (en) * | 2011-12-12 | 2012-06-27 | 北京陆桥技术有限责任公司 | Listeria monocytogenes nucleic acid chromatography detection kit, its detection method and application thereof |
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2013
- 2013-12-10 CN CN201310670347.2A patent/CN103773845B/en not_active Expired - Fee Related
Patent Citations (3)
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CN1811447A (en) * | 2006-02-08 | 2006-08-02 | 杭州优思达生物技术有限公司 | Nucleic acid membrane chromatographic fast detecting method and its test paper bar and use thereof |
CN102520172A (en) * | 2011-12-12 | 2012-06-27 | 北京陆桥技术有限责任公司 | Listeria monocytogenes nucleic acid chromatography detection kit, its detection method and application thereof |
CN103255217A (en) * | 2013-04-25 | 2013-08-21 | 天津出入境检验检疫局动植物与食品检测中心 | Side direction current test strip detection kit for detecting canis familiaris component in feed and application thereof |
Non-Patent Citations (2)
Title |
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DIANC KOZWICH,ET AL: "Development of a novel,rapid integrated cryptosporidium parvum detection assay", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 * |
石丰运: "应用基因芯片技术鉴别检测动物源性成分", 《中国优秀硕士学位论文全文数据库农业科学辑》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104017872A (en) * | 2014-05-29 | 2014-09-03 | 天津出入境检验检疫局动植物与食品检测中心 | Lateral flow test paper strip detection kit for detection of fox source components in food and feed and application thereof |
CN104017872B (en) * | 2014-05-29 | 2017-02-08 | 天津出入境检验检疫局动植物与食品检测中心 | Lateral flow test paper strip detection kit for detection of fox source components in food and feed and application thereof |
CN114686600A (en) * | 2022-02-24 | 2022-07-01 | 宁波大学 | Primer group and method for meat detection based on seven-fold PCR technology |
CN114686600B (en) * | 2022-02-24 | 2023-12-12 | 宁波大学 | Primer group and method for meat detection based on seven-fold PCR technology |
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