CN103773713A - Microbial preparation with high biological membrane culturing efficiency and preparation method thereof - Google Patents
Microbial preparation with high biological membrane culturing efficiency and preparation method thereof Download PDFInfo
- Publication number
- CN103773713A CN103773713A CN201310671373.7A CN201310671373A CN103773713A CN 103773713 A CN103773713 A CN 103773713A CN 201310671373 A CN201310671373 A CN 201310671373A CN 103773713 A CN103773713 A CN 103773713A
- Authority
- CN
- China
- Prior art keywords
- preparation
- microbial
- aerobic
- substratum
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a microbial preparation with high biological membrane culturing efficiency and a preparation method thereof. The preparation method of the microbial preparation comprises the following steps: performing aerobic processing or fermentation to obtain microbial inoculums after respectively mixing bacillus subtilis, rhodopseudanonas palustris, nitrospina sap and brachymonas denitrificans with a culture medium, and mixing the microbial inoculums according to a ratio to obtain the required microbial preparation, wherein the microbial preparation comprises 5-30 percent of bacillus subtilis, 10-40 percent of rhodopseudanonas palustris, 10-60 percent of nitrospina sap and 10-40 percent of brachymonas denitrificans. The preparation method disclosed by the invention is capable of performing large-scale production, high in efficiency and low in cost; multiple microbial inoculums can be simultaneously prepared through fermentation, and thus the time is shortened. A film prepared by adopting the microbial preparation is high in cell density, good in mass transfer property, stable, and long in validity period, and can be repeatedly used; the microbial preparation is capable of increasing the growth speed of a biological membrane, shortening the biological membrane culturing time, and increasing the quantity of advantage microorganisms in the biological membrane.
Description
Technical field
The present invention relates to a kind of microbial preparation, relate in particular to a kind of microbial preparation with high Biofilm Colonization efficiency and preparation method thereof.
Background technology
Biomembranous using method is to make wastewater streams outgrowth at the lip-deep microbial film of fixed support thing, utilizes biological oxidation and each alternate exchange of substance, and the method for organic pollutant in degrading waste water, is the one of waste water aerobiont facture.The structures of processing waste water with biomembrance process have biological filter, blodisc and biological contact oxidation pond etc.The ecosystem that microbial film is made up of aerobic bacteria, anerobe, amphimicrobe, fungi, protozoon and the algae etc. of highly dense, its solid dielectric adhering to is called filtrate or carrier.
Microbial film outwards can be divided into be sick of layer, good gas-bearing formation, adhere to water layer, motion water layer from filtrate.The action principle of biomembrance process is, first microbial film adsorbs and adheres to water layer organism, is decomposed, then enter the lonely layer of decomposition of being sick of by the aerobic organism of good gas-bearing formation, mobile water layer washes out by aging microbial film the new microbial film of growing, and so forth to reach the object of purifying waste water.
Patent CN1928081B discloses a kind of artificial biological film and preparation method.After adopting beneficial microbe strain fermentation, concentrate.With polyvinyl alcohol, sodium alginate, Xylo-Mucine etc., film forming starting material are soluble in water makes after glue, after again active carbon from coal powder, diatomite powder, sodium-acetate being mixed with individual plant or many strains microorganism cells, add in glue, then be placed in spraying plant pressurized spray and splash in powder bed fixingly, form artificial biological film.
Biomembranous preparation growth can also be biofilm, and biofilm refers to after the microorganic adhesion in sewage, the slowly process of growth.The time of growth generally needs 15 days-20 days ability biofilms to complete.Microbe species on microbial film is a lot, has bacterium, fungi, algae, protozoon and metazoan, and macroscopic microfauna.Biological filter at the middle and upper levels, middle level, lower floor form biomembranous microorganism, and kind is also had any different, and therefore the time of its biofilm is also not quite similar.The overlong time of biofilm, is unfavorable for biomembranous widespread use and recycles for a long time.
In order to solve the problem that the biofilm time is long, operation is more, patent CN202829708U discloses a kind of method of suspended packing microbial quick film forming.It is characterized in that first mud being fetched rear aeration 48h, do and inoculate bacterium with the mud after aeration, be inoculated in the sequence batch (sbr reactor device that floating stuffing is housed, aeration 6h, staticly settles 6h; Then add nutraceutical matrix, aeration 10h, staticly settles 2h as a cycle of operation, moves 2 cycles every day, and carrying out during this time spoil disposal, to keep sludge suspension solids concn to remain on a certain concentration value within the scope of 2-20g/L constant; Operation 7-10d biofilm completes.
Above-mentioned patent is mainly to improve in working method, flow process, although improved the speed of biofilm, but makes the operation of original biofilm more complicated, is unfavorable for promoting the use of.
Summary of the invention
The present invention seeks to biotechnological formulation of a kind of effective raising Biofilm Colonization efficiency and preparation method thereof.
For achieving the above object, first aspect of the present invention provides a kind of preparation method of the biotechnological formulation with high Biofilm Colonization efficiency, and concrete steps comprise:
In above-mentioned step 1, the mixed weight ratio of the front subtilis of aerobic fermentation and substratum is preferably 1-10:90-99, more preferably 1-3:97-99.
In above-mentioned step 1, the temperature of aerobic aerobic fermentation is preferably 30-39 ℃, and more preferably 35-38 ℃ most preferably is 37 ℃, and fermentation time is preferably 1-5 days, and more preferably 1-3 days most preferably is 2 days.
Subtilis described in above-mentioned step 1 (Bacillus subtilis) is preferably the bacterial classification that deposit number is No.ACCC10619.
Substratum described in above-mentioned step 1 is at least two kinds of mixtures in bean cake powder, peptone, Semen Maydis powder, sucrose, potassium primary phosphate, Sodium phosphate dibasic, ammonium sulfate, urea or sterilized water.
Substratum in the aerobic aerobic fermentation culturing process of subtilis described in above-mentioned step 1, according to weight percent proportioning, comprising: bean cake powder 2.0-3.5%, peptone 0-1.0%, Semen Maydis powder 3-4%, glucose 0-4%, Sodium phosphate dibasic 0-1%, potassium primary phosphate 0-1%, ammonium sulfate 0-2%, urea 0-1%, water 82.5-95%.
Before anaerobism illumination fermentation described in above-mentioned step 1, Rhodopseudomonas palustris and substratum mixed weight ratio are preferably 1-15:85-99, more preferably 2-13:90-99, more preferably 5-10:90-95.
In above-mentioned step 1, the temperature of anaerobism illumination fermentation is preferably 30-38 ℃, more preferably 30-35 ℃, and more preferably 32 ℃, the time of anaerobism illumination fermentation is preferably 2-10 days, and more preferably 3-8 days, most preferably is 5 days.
Rhodopseudomonas palustris described in above-mentioned step 1 (Rhodopseudomonas palustris) is preferably the bacterial classification that deposit number is No.ACCC10649.
Substratum described in above-mentioned step 1 is at least two kinds of mixtures in yeast powder, sodium acetate, ammonium sulfate, potassium primary phosphate, magnesium sulfate, sodium-chlor or sterilized water.
Substratum in Rhodopseudomonas palustris anaerobism illumination fermentation culture process described in above-mentioned step 1, according to weight percent proportioning, comprising: yeast powder 1-2%, sodium acetate 1-5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, magnesium sulfate 0-1%, sodium-chlor 0.5-1%, water 87-99.2%.
In above-mentioned step 1, the mixed weight ratio of nitrated thorn bacterium and substratum is preferably 1-15:85-99, more preferably 3-12:90-99, more preferably 5-10:90-95.
The temperature that nitrated thorn bacterium described in above-mentioned step 1 is fermented is preferably 25-35 ℃, and more preferably 25-30 ℃, most preferably is 28 ℃, and the time of fermentation is preferably 2-7 days, and more preferably 2-5 days most preferably is 3 days.
Nitrated thorn bacterium (Nitrospina sp) described in above-mentioned step 1 is preferably the bacterial classification that deposit number is No.MCCC1A00556.
Substratum described in above-mentioned step 1 is at least two kinds of mixtures of sodium bicarbonate, Sodium Nitrite, sodium carbonate, potassium primary phosphate, magnesium sulfate, sodium-chlor, sterilized water.
Substratum in nitrated thorn bacterium anaerobism illumination fermentation culture process described in above-mentioned step 1, according to weight percent proportioning, comprising: sodium bicarbonate 0.1-0.5%, Sodium Nitrite 0.1-0.5%, sodium carbonate 0.1-0.5%, potassium primary phosphate 0-1%, magnesium sulfate 0-1%, sodium-chlor 0.5-1%, water 95.5-96.5%.
In above-mentioned step 1, the mixed weight ratio of denitrification brachyplast Zymomonas mobilis and substratum is preferably 1-8:92-99, more preferably 1-5:95-99, more preferably 1-2:98-99.
The temperature of the fermentation described in above-mentioned step 1 is preferably 25-35 ℃, and more preferably 25-30 ℃ most preferably is 28 ℃, and the time of described fermentation is preferably 1-8 days, more preferably 1-5 days, and more preferably 2-4 days, most preferably is 3 days.
Denitrification brachyplast Zymomonas mobilis (Brachymonas denitrificans) described in above-mentioned step 1 is preferably the bacterial classification that deposit number is No.CGMCC1.7100.
Substratum described in above-mentioned step 1 is at least two kinds of mixtures in sodium-acetate, saltpetre, potassium primary phosphate, magnesium chloride, calcium chloride or sterilized water.
Substratum in the aerobic aerobic fermentation culturing process of denitrification brachyplast Zymomonas mobilis described in above-mentioned step 1, according to weight percent proportioning, comprising: sodium-acetate 0.01-0.05%, saltpetre 0.01-0.05%, potassium primary phosphate 0.001-0.002%, magnesium chloride 0.001-0.002%, calcium chloride 0.001-0.002%, water 99.894-99.97%.
A second aspect of the present invention is to provide a kind of microbial preparation that adopts above-mentioned method to prepare, and comprises microbial components, take microbial components total amount as benchmark, comprises the bacterial classification of following weight percent:
Bacillus subtilis microbial agent is preferably 5-30%, more preferably 10-25%, more preferably 10-20%;
Rhodopseudomonas palustris microbial inoculum is preferably 10-40%, more preferably 10-30%, more preferably 20-30%;
Nitrated thorn bacteria agent is preferably 10-60%, more preferably 20-50%, more preferably 30-40%;
Denitrification brachyplast Zymomonas mobilis microbial inoculum 10-40%, more preferably 20-40%, more preferably 20-30%.
The preparation method of the microbial preparation with high Biofilm Colonization efficiency of the present invention can accomplish scale production; process is simple, efficiency is high and cost is low; the fermentation preparation of multiple microbial inoculum can be carried out simultaneously, thereby greatly shortens the preparation time of microbial preparation, improves the efficiency of producing.
Adopt that in the film that microbial preparation of the present invention obtains, cell density is high, mass transfer performances good, stable, validity period long, reusable, also have and can improve the biomembranous speed of growth, shorten the biomembranous biofilm time, improve the advantage of the quantity of the superior microorganism of the necessary aerobic anaerobic in microbial film.
Accompanying drawing explanation
Fig. 1 contrasts COD between the aerobic incubation period of 0% inoculum size to remove situation temporal evolution figure in the embodiment of the present invention 3;
Fig. 2 contrasts COD between the aerobic incubation period of 0.05% inoculum size to remove situation temporal evolution figure in the embodiment of the present invention 3;
Fig. 3 contrasts COD between the aerobic incubation period of 0.075% inoculum size to remove situation temporal evolution figure in the embodiment of the present invention 3;
Fig. 4 contrasts COD between the aerobic incubation period of 0.1% inoculum size to remove situation temporal evolution figure in the embodiment of the present invention 3;
Fig. 5 contrasts COD between 0% inoculum size anaerobism incubation period to remove situation temporal evolution figure in the embodiment of the present invention 3;
Fig. 6 contrasts COD between 0.05% inoculum size anaerobism incubation period to remove situation temporal evolution figure in the embodiment of the present invention 3;
Fig. 7 contrasts COD between 0.075% inoculum size anaerobism incubation period to remove situation temporal evolution figure in the embodiment of the present invention 3.
Specific embodiment
The biomembrance process advantages such as to have the mud of generation few because of it, and operational management is convenient, and processing costs is low, in sewage disposal, especially breeding wastewater processing aspect has unique advantage.Belong to together or similar microorganism by interpolation, to the biomembranous growth efficiency promoter action that can improve, also can shorten the time of its biofilm simultaneously.Therefore, utilize suitable microbiobacterial agent can effectively improve the biomembranous speed of growth, improve the quantity of the superior microorganism of the necessary aerobic anaerobic in microbial film, thus the time of improving biomembranous quality and less biofilm.
Below in conjunction with the drawings and specific embodiments, the present invention will be further described, but not as limiting to the invention.
The bacterial classification that subtilis described in the present embodiment (Bacillus subtilis) is No.ACCC10619 for deposit number.
The bacterial classification that described Rhodopseudomonas palustris (Rhodopseudomonas palustris) is No.ACCC10649 for deposit number.
The bacterial classification that described nitrated thorn bacterium (Nitrospina sp) is No.MCCC1A00556 for deposit number.
The bacterial classification that described denitrification brachyplast Zymomonas mobilis (Brachymonas denitrificans) is No.CGMCC1.7100 for deposit number.
Preparation can have the method for the microbial preparation of high Biofilm Colonization efficiency, and its concrete steps are as follows:
Before aerobic fermentation by the preparation of raw material of following weight per-cent: subtilis 1, %, substratum 99%, carry out 37 ℃ of aerobic aerobic fermentations 2 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 1, the weight percent proportioning of substratum is: bean cake powder 2.0%, peptone 1.0%, Semen Maydis powder 3.0%, Sodium phosphate dibasic 0.75%, potassium primary phosphate 0.2%, water 93.05%.
Before anaerobism illumination fermentation by the preparation of raw material of following weight per-cent: Rhodopseudomonas palustris 5%, substratum 95%, carry out 32 ℃ of anaerobism illumination and ferment 5 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 2, the weight percent proportioning of substratum is: yeast powder 1.0%, sodium acetate 5.0%, ammonium sulfate 3.0%, sodium-chlor 0.5%, water 90.5%.
Before aerobic fermentation by the preparation of raw material of following weight per-cent: nitrated thorn bacterium 10%, substratum 90%, carry out 28 ℃ of aerobic aerobic fermentations 3 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 3, the weight percent proportioning of substratum is: sodium bicarbonate 0.5%, Sodium Nitrite 0.1%, sodium carbonate 0.5%, magnesium sulfate 0.5%, sodium-chlor 0.5%, water 97.9%.
Before aerobic fermentation by the preparation of raw material of following weight per-cent: denitrification brachyplast Zymomonas mobilis 1%, substratum 99%, carry out 28 ℃ of aerobic aerobic fermentations 3 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 4, the weight percent proportioning of substratum is: sodium-acetate 0.05%, saltpetre 0.05%, potassium primary phosphate 0.002%, magnesium chloride 0.001%, calcium chloride 0.001%, water 99.896%.
After mixing, obtain required microbial preparation.
The bacterial classification that subtilis described in the present embodiment (Bacillus subtilis) is No.ACCC10619 for deposit number.
The bacterial classification that described Rhodopseudomonas palustris (Rhodopseudomonas palustris) is No.ACCC10649 for deposit number.
The bacterial classification that described nitrated thorn bacterium (Nitrospina sp) is No.MCCC1A00556 for deposit number.
The bacterial classification that described denitrification brachyplast Zymomonas mobilis (Brachymonas denitrificans) is No.CGMCC1.7100 for deposit number.
Preparation has the method for the microbial preparation of high Biofilm Colonization efficiency, and its concrete steps are as follows:
Before aerobic fermentation by the preparation of raw material of following weight per-cent: subtilis 3%, substratum 97%, carry out 37 ℃ of aerobic aerobic fermentations 2 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 1, the weight percent proportioning of substratum is: bean cake powder 3.0%, Semen Maydis powder 4.0%, Sodium phosphate dibasic 0.75%, potassium primary phosphate 0.2%, ammonium sulfate 0.5%, water 91.55%.
Before anaerobism illumination fermentation by the preparation of raw material of following weight per-cent: Rhodopseudomonas palustris 10%, substratum 90%, carry out 32 ℃ of anaerobism illumination and ferment 5 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 2, the weight percent proportioning of substratum is: yeast powder 2.0%, sodium acetate 5.0%, potassium primary phosphate 0.05%, magnesium sulfate 0.05%, sodium-chlor 0.5%, water 92.4%.
Before aerobic fermentation by the preparation of raw material of following weight per-cent: nitrated thorn bacterium 10%, substratum 90%, carry out 28 ℃ of aerobic aerobic fermentations 3 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 3, the weight percent proportioning of substratum is: sodium bicarbonate 0.5%, Sodium Nitrite 0.5%, potassium primary phosphate 0.5%, magnesium sulfate 0.5%, sodium-chlor 0.5%, water 97.5%.
Before aerobic fermentation by the preparation of raw material of following weight per-cent: denitrification brachyplast Zymomonas mobilis 2%, substratum 98%, carry out 28 ℃ of aerobic aerobic fermentations 3 days.Fermentation secondary fermentation liquid is for subsequent use.
In described step 4, the weight percent proportioning of substratum is: sodium-acetate 0.05%, saltpetre 0.03%, potassium primary phosphate 0.002%, calcium chloride 0.002%, water 99.916%.
Bacillus subtilis microbial agent, Rhodopseudomonas palustris microbial inoculum, nitrated thorn bacteria agent and denitrification brachyplast Zymomonas mobilis microbial inoculum are mixed, and its weight percent is: bacillus subtilis microbial agent 10%, Rhodopseudomonas palustris microbial inoculum 30%, nitrated thorn bacteria agent 30%, denitrification brachyplast Zymomonas mobilis microbial inoculum 30%.
Microbial preparation of the present invention affects the experiment of the Biofilm Colonization efficiency under anaerobism, aerobic environment.
Experiment purpose: composite bacteria is inoculated in the reactor of anaerobic-aerobic combination, and adds through sedimentation or coagulation pretreatment pig farm breeding wastewater, by inoculation composite bacteria, carry out biofilm test.Test according to the difference before and after adding, investigate it for shortening biofilm time effect and improving the effect of removing pollutent.
Experiment sewage: this tests livestock breeding wastewater used, and its major traits is water turbidity and contains certain impurity, it is dark brown and with certain stink that color is.The main water-quality guideline COD of this experiment test sewage, its scope is in 3000mg/L left and right.
Experimental installation: this tests experimental installation used is the rectangular parallelepiped made from synthetic glass, its specification is 255mm × 65mm × 225mm, comprises three independently lattice chambers in reactor, and each lattice chamber is a little reactor, in each lattice chamber, filler is housed, for culturing micro-organisms.Waste water is housed in reactor, microorganism is cultivated to domestication, inject simulation Wastewater from Pig Farm, utilize microorganism to remove the COD in waste water.Aeration or sealing are carried out according to test requirements document (aerobic or anaerobism) in each lattice chamber.
Experimental technique: in this experiment, biomembranous cultivation and domestication are carried out on filler, so first filler is fixed in each junior unit of reactor, by fastening, it is hung vertically in each little lattice uniformly.Then in each little lattice, add aerating apparatus, for the required oxygen of microorganism growth.The test of two groups of anaerobic and aerobic condition is carried out respectively in this experiment to the cultivation of microorganism.
First stage is culturing micro-organisms under aerobic condition, in two little lattice, add manual simulation's breeding wastewater (to propagate waste water artificially and add tap water preparation by sucrose, ammonium chloride and potassium primary phosphate respectively, making its C:N:P is 100:5:1, add wherein again the breeding wastewater of 100mL, make its COD remain on 1000mg/L left and right) (volume pump is not intake to adopt interrupter method culturing micro-organisms, but continuous aeration), according to 0.05%, 0.075% and 0.1% 3 inoculative proportion access bacterial classification, approximately cultivate aerobic biofilm and finish for one week.Be specially and add analog culture waste water, continuous aeration is inoculated on filler, and influent COD remains on 1000mg/L left and right, carries out interrupter method culturing micro-organisms, test every other day Inlet and outlet water COD numerical value, after one week, determine that take COD clearance as basis for estimation the aerobic biofilm stage finishes and time point.
Subordinate phase is under anaerobic to cultivate microbial film.Reactor the first lattice stop aeration, meet anaerobic condition, the second lattice aeration.Adopt interrupter method to cultivate anaerobion (volume pump is not intake), add and propagate waste water (the same constant 1000mg/L of concentration left and right) artificially, according to 0.05%, 0.075% and 0.1% 3 inoculative proportion access bacterial classification, approximately cultivate anaerobism biofilm 10d and finish.Be specially when aerobic microbiological and cultivate maturation, on the basis of aerobic microbiological, cultivate anaerobion, remove aerator, stop aeration, water distribution concentration increases to and is greater than 1000mg/L, test Inlet and outlet water COD, and after 10 days, anaerobic bacterium is cultivated and finishes.
The mixed weight ratio of the composite bacteria that experiment is used, the numerical value of inoculum size are as shown in table 1-2.
Microbial preparation of the present invention aerobic, anaerobism biofilm number of days is as shown in table 3.
Table 1, many bacterial classification inoculation part by weight list for experiment
| Strain name | Bacteria containing amount | Proportioning |
| Rhodopseudomonas palustris | 2000000000/ml | 30% |
| Nitrated thorn bacterium | 0.1 hundred million/ml | 30% |
| Denitrification brachyplast Zymomonas mobilis | 0.1 hundred million/ml | 30% |
| Subtilis | 5000000000/ |
10% |
Table 2, the list of composite bacteria liquid inoculum size
Table 3, aerobic, the anaerobism biofilm number of days synopsis of microbial preparation of the present invention
About COD between aerobic incubation period removes the removal situation result of COD between situation and anaerobism incubation period: as shown in Fig. 1-7.
Interpretation:
(1) between relevant aerobic incubation period, COD removes situation temporal evolution trend.Fig. 1-4th, between aerobic incubation period, COD removes situation temporal evolution figure, and as shown and be about 1000mg/L along with influent COD remains on concentration in Fig. 1-4, water outlet COD value has obvious downtrending, and COD clearance increases gradually.Be that 1052mg/L starts from starting influent COD, clearance along with the time increases gradually, reaches 96.06% of clearance maximum from 9.89% in the time that influent COD is 1022.5mg/L after one week, indicates that aerobic stage cultivation aerobic microbiological reaches the requirement of purifying waste water.On filler, microbial film color deepens to some extent during this time, and from the thread yellow browning look again that gradually becomes of white, film thickness obviously increases, microorganism culturing success.
And comparison diagram 1-4 can find out, along with the increase of microbial preparation inoculum size of the present invention, COD removal efficiency obviously improves, and microbial preparation of the present invention can make COD removal efficiency tool after four days improve significantly.
(2) comparison diagram 5-7, along with the increase of microbial preparation inoculum size of the present invention, COD removal efficiency was also significantly improved since the 6th day; In first 6 days, after entering anaerobic environment, the amphimicrobe of growing needs an adaptive process under aerobic condition, COD removal efficiency variation tendency is not obvious, but even so, even in this process, the oxygen of trace also can make amphimicrobe increased activity (as shown in Figure 6 and Figure 7), therefore formed the phenomenon that acidifying bacterium breeds from top to bottom.Different vaccination amount is for the consumption of COD under anaerobic condition and be biomembranously formed with impact.
(3) from table 2,3 is known, to aerobic, different bacterial classification inoculum sizes is set under anaerobic condition, can see having any different in final biomembranous formation time, under good oxygen condition when inoculum size is 0.075% time, the microbial film rise time compared with control group time shorten 1 day and cost also lower than 0.1% inoculum size group; Under anaerobic state, it is 0.1% inoculum size effect optimum.Under aerobic condition 0.075%, 0.1% inoculum size and under anaerobic 0.1% inoculum size all contribute to reduce the biofilm time, reduce processing cost.
In sum, in embodiments of the present invention, in two groups of experiments of aerobic-anaerobic, all carried out bacterial classification interpolation, in the bacterial classification of interpolation, the bacteria containing amount of composite bacteria liquid is 2,000,000,000/ml, successfully realize aerobic and cultivation anaerobe film, thereby effectively improved Biofilm Colonization efficiency.
Above specific embodiments of the invention be have been described in detail, but it is as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the modification done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (10)
1. a preparation method with the microbial preparation of high Biofilm Colonization efficiency, is characterized in that, concrete steps comprise:
Step 1, subtilis, nitrated thorn bacterium, denitrification brachyplast Zymomonas mobilis carry out separately respectively aerobic aerobic fermentation and cultivate to obtain microbial inoculum, and Rhodopseudomonas palustris carries out anaerobism illumination fermentation culture and obtains microbial inoculum;
Step 2, each microbial inoculum that step 1 is obtained mixes, obtain required biotechnological formulation, wherein, the part by weight that each microbial inoculum mixes is bacillus subtilis microbial agent 5-30%, Rhodopseudomonas palustris microbial inoculum 10-40%, nitrated thorn bacteria agent 10-60%, denitrification brachyplast Zymomonas mobilis microbial inoculum 10-40%.
2. preparation method according to claim 1, it is characterized in that, substratum in the aerobic aerobic fermentation culturing process of subtilis described in step 1, according to weight percent proportioning, comprising: bean cake powder 2.0-3.5%, peptone 0-1.0%, Semen Maydis powder 3-4%, glucose 0-4%, Sodium phosphate dibasic 0-1%, potassium primary phosphate 0-1%, ammonium sulfate 0-2%, urea 0-1%, water 82.5-95%.
3. preparation method according to claim 2, is characterized in that, the weight ratio that the subtilis described in step 1 mixes with substratum is 1-10:90-99, and the temperature of aerobic aerobic fermentation is 30-39 ℃, and the time is 1-3 days.
4. preparation method according to claim 1, it is characterized in that, substratum in Rhodopseudomonas palustris anaerobism illumination fermentation culture process described in step 1, according to weight percent proportioning, comprising: yeast powder 1-2%, sodium acetate 1-5%, ammonium sulfate 1-3%, potassium primary phosphate 0-1%, magnesium sulfate 0-1%, sodium-chlor 0.5-1%, water 87-99.2%.
5. preparation method according to claim 4, is characterized in that, the weight ratio that the Rhodopseudomonas palustris described in step 1 mixes with substratum is 1-15:85-99, and the temperature of anaerobism illumination fermentation is 30-38 ℃, and the time is 2-10 days.
6. preparation method according to claim 1, it is characterized in that, substratum in nitrated thorn bacterium anaerobism illumination fermentation culture process described in step 1, according to weight percent proportioning, comprising: sodium bicarbonate 0.1-0.5%, Sodium Nitrite 0.1-0.5%, sodium carbonate 0.1-0.5%, potassium primary phosphate 0-1%, magnesium sulfate 0-1%, sodium-chlor 0.5-1%, water 95.5-96.5%.
7. preparation method according to claim 6, is characterized in that, the weight ratio that the nitrated thorn bacterium described in step 1 mixes with substratum is 3-12:90-99, and the temperature of aerobic aerobic fermentation is 25-35 ℃, and the time is 2-7 days.
8. preparation method according to claim 1, it is characterized in that, substratum in the aerobic aerobic fermentation culturing process of denitrification brachyplast Zymomonas mobilis described in step 1, according to weight percent proportioning, comprising: sodium-acetate 0.01-0.05%, saltpetre 0.01-0.05%, potassium primary phosphate 0.001-0.002%, magnesium chloride 0.001-0.002%, calcium chloride 0.001-0.002%, water 99.894-99.97%.
9. preparation method according to claim 8, is characterized in that, the weight ratio that the denitrification brachyplast Zymomonas mobilis described in step 1 mixes with substratum is 1-8:92-99, and the temperature of aerobic aerobic fermentation is 25-35 ℃, and the time is 1-8 days.
10. a microbial preparation that adopts method claimed in claim 1 to prepare, is characterized in that, comprises microbial components, take microbial components total amount as benchmark, comprises the bacterial classification of following weight percent:
Bacillus subtilis microbial agent 5-30%, Rhodopseudomonas palustris microbial inoculum 10-40%, nitrated thorn bacteria agent 10-60%, denitrification brachyplast Zymomonas mobilis microbial inoculum 10-40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310671373.7A CN103773713B (en) | 2013-12-10 | 2013-12-10 | There is microorganism formulation of high Biofilm Colonization efficiency and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310671373.7A CN103773713B (en) | 2013-12-10 | 2013-12-10 | There is microorganism formulation of high Biofilm Colonization efficiency and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103773713A true CN103773713A (en) | 2014-05-07 |
| CN103773713B CN103773713B (en) | 2016-08-17 |
Family
ID=50566480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310671373.7A Active CN103773713B (en) | 2013-12-10 | 2013-12-10 | There is microorganism formulation of high Biofilm Colonization efficiency and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103773713B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132340A (en) * | 2015-10-14 | 2015-12-09 | 武汉瑞泽园生物环保科技有限公司 | Compound microorganism water purifying bactericide and preparing method thereof |
| CN113322203A (en) * | 2021-05-31 | 2021-08-31 | 青岛万慧源环保科技有限公司 | Composite microbial inoculum for aerobic denitrification and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376877A (en) * | 2007-08-31 | 2009-03-04 | 天津中敖生物科技有限公司 | Compound microecological preparation for purifying cultivation water, preparation formulation thereof and preparation processes of the preparation and the preparation formulation |
| CN101698539A (en) * | 2009-10-13 | 2010-04-28 | 王颖 | Microecological agent for purifying water and preparation method thereof |
| CN101905985A (en) * | 2010-07-30 | 2010-12-08 | 河北省微生物研究所 | Method for preparing microorganism-decomposing agent used for producing biological organic fertilizer by livestock and poultry manure fermentation |
| CN102168045A (en) * | 2010-12-24 | 2011-08-31 | 北京科为博生物科技有限公司 | Bacillus subtilis preparation and preparation method thereof |
| CN102718327B (en) * | 2012-07-05 | 2013-11-06 | 浙江皇冠科技有限公司 | Nano-biological water body remediation agent for aquaculture and preparation method thereof |
-
2013
- 2013-12-10 CN CN201310671373.7A patent/CN103773713B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101376877A (en) * | 2007-08-31 | 2009-03-04 | 天津中敖生物科技有限公司 | Compound microecological preparation for purifying cultivation water, preparation formulation thereof and preparation processes of the preparation and the preparation formulation |
| CN101698539A (en) * | 2009-10-13 | 2010-04-28 | 王颖 | Microecological agent for purifying water and preparation method thereof |
| CN101905985A (en) * | 2010-07-30 | 2010-12-08 | 河北省微生物研究所 | Method for preparing microorganism-decomposing agent used for producing biological organic fertilizer by livestock and poultry manure fermentation |
| CN102168045A (en) * | 2010-12-24 | 2011-08-31 | 北京科为博生物科技有限公司 | Bacillus subtilis preparation and preparation method thereof |
| CN102718327B (en) * | 2012-07-05 | 2013-11-06 | 浙江皇冠科技有限公司 | Nano-biological water body remediation agent for aquaculture and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132340A (en) * | 2015-10-14 | 2015-12-09 | 武汉瑞泽园生物环保科技有限公司 | Compound microorganism water purifying bactericide and preparing method thereof |
| CN105132340B (en) * | 2015-10-14 | 2018-03-30 | 武汉瑞泽园生物环保科技有限公司 | Complex microorganism water purification microbial inoculum and preparation method thereof |
| CN113322203A (en) * | 2021-05-31 | 2021-08-31 | 青岛万慧源环保科技有限公司 | Composite microbial inoculum for aerobic denitrification and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103773713B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xie et al. | Biodiesel production with the simultaneous removal of nitrogen, phosphorus and COD in microalgal-bacterial communities for the treatment of anaerobic digestion effluent in photobioreactors | |
| Tang et al. | Performance and mechanism of a novel algal-bacterial symbiosis system based on sequencing batch suspended biofilm reactor treating domestic wastewater | |
| Zheng et al. | Substrates removal and growth kinetic characteristics of a heterotrophic nitrifying-aerobic denitrifying bacterium, Acinetobacter harbinensis HITLi7T at 2 C | |
| CN103896407B (en) | A kind of quick startup, biofilm carbon antimicrobial composition process for purifying water | |
| Xu et al. | The impact of seasonal variations about temperature and photoperiod on the treatment of municipal wastewater by algae-bacteria system in lab-scale | |
| CN103159529B (en) | Technical method for biological fermentation of liquid dung and biogas slurry | |
| CN111088179B (en) | Compound microbial agent for ecological restoration and preparation method thereof | |
| CN105174476A (en) | Activated sludge and microalgae coupled granular system for waste water treatment and establishment and operation method thereof | |
| CN102976497A (en) | Method for treating high-concentration organic waste water by bacilli | |
| CN106676022A (en) | Method for preparing microbe bacteria liquid for treating black-odor riverway | |
| CN104140935A (en) | Denitrification rhodococcus and rhodococcus microbial agent production method and application | |
| CN103103130A (en) | Production method for lipid through mixed culture of microbes | |
| CN102190373B (en) | Aerobic and facultative anaerobic microbial activated bactericide for water treatment and application thereof in contact oxidation process | |
| CN109942091B (en) | Bacterial-algae attached biological fiber bed, preparation method thereof and method for strengthening treatment of pig wastewater for nitrogen and phosphorus removal | |
| CN109694828A (en) | The comprehensive processing technique of threonine mother liquor | |
| CN102776140B (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| CN103103129A (en) | Production method for lipid through synchronous mixed culture of microbes | |
| CN103305443B (en) | Preparation and method for restoring ammonia nitrogen-containing industrial sewage | |
| CN108865924A (en) | Heterotrophic nitrification-biological aerobic denitrification pseudomonad microbial inoculum and preparation method thereof, application | |
| CN102220240A (en) | PM-I sludge reduction microbial agent | |
| CN101386822B (en) | Special effect phosphate accumulating organisms and waste water processing method using thereof | |
| CN108342338B (en) | Method for treating pharmaceutical wastewater containing antibiotics | |
| CN110272853B (en) | Microbial culture method | |
| CN101029298A (en) | Production of efficient microbe bacteria combing agent | |
| CN103773713A (en) | Microbial preparation with high biological membrane culturing efficiency and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200513 Address after: 201100 room J43, floor 2, building 11, No. 988, Zhongchun Road, Minhang District, Shanghai Patentee after: SHANGHAI CHUANGBO ECOLOGICAL ENGINEERING Co.,Ltd. Address before: 201108, room 111, building D, No. 4299 Jin Du Road, Shanghai, Minhang District Patentee before: SHANGHAI GENZHUO BIOLOGICAL ENGINEERING Co.,Ltd. |
|
| TR01 | Transfer of patent right |