CN103766219A - Method for tissue culture of long-ear kniphofia - Google Patents
Method for tissue culture of long-ear kniphofia Download PDFInfo
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- CN103766219A CN103766219A CN201410024946.1A CN201410024946A CN103766219A CN 103766219 A CN103766219 A CN 103766219A CN 201410024946 A CN201410024946 A CN 201410024946A CN 103766219 A CN103766219 A CN 103766219A
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Abstract
The invention discloses a method for the tissue culture of long-ear kniphofia. The method comprises the steps of picking up long-ear kniphofia budlet, washing, culturing by a bud induction medium, putting cultured calluses with buds into a multiplication medium for differentiation multiplication, carrying out adventitious bud cultivation and rooting cultivation, and obtaining the long-ear kniphofia plant through the seedling hardening technology and the transplanting technology. Compared with the prior art, according to the tissue culture technology, the maturing rate can be improved, and the different nutrition facts can be provided at the different cultivation stages by the selected bud induction culture medium, multiplication culture medium, seedling boosting culture medium and rooting culture medium, so that the original female characters can be kept to the maximum, and the transplanting survival rate in the steps of seedling hardening and transplanting can reach 90%.
Description
Technical field
The present invention relates to plant tissue culture technology, especially relate to a kind of method of cultivating long fringe torch flower of organizing.
Background technology
Long fringe torch flower is Liliaceae torch Pittosporum herbaceos perennial.80～120 centimetres of plant heights, stem is upright.Raceme raw hundreds of tubular little Hua, is torch-shaped, corolla Chinese red, 6～July of florescence.Like warmly, should grow in loose fertile sandy loam.The scape that very sends out is high has held up the inflorescence as torch flower, glorious considerable, is applicable to the perennial mixed border of layout and configures before building, and group planting is among lawn or plant by rockwork, is used as entourage, also can make cut-flower.Long fringe torch flower is longer than general torch flower inflorescence, but ripening rate is very low, and plant division is slow, therefore need to develop tissue culture technology, improve the regularity of seedling propagation speed and seedling and keep original maternal character, realize the batch production of growing seedlings, at present, the group training report of long fringe torch flower there is not yet.
Summary of the invention
Object of the present invention is exactly to provide a kind of transplanting survival rate can reach 90% tissue to cultivate the colored method of long fringe torch in order to overcome the defect that above-mentioned prior art exists.
Object of the present invention can be achieved through the following technical solutions:
Organize a method of cultivating long fringe torch flower, adopt following steps:
(1) bud induction is cultivated
Win long fringe torch flower budlet, after cleaning, 2h soaks 15min, aseptic water washing 5-6 time with alcohol immersion 30s, 1wt ‰ mercuric chloride of 75v/v%, then blot surperficial moisture content, get tender shoots base portion and be cut into 1cm length, be inoculated on bud inducing culture, control cultivation temperature is 24-28 ℃, and illumination is 70-100 μ mol/ms, carries out bud induction and cultivates;
(2) bud differentiation and proliferation
After tender shoots is inoculated on bud inducing culture 5 weeks, bastem portion starts to expand and occurs yellow green projection, after 3 weeks, visible significantly callus continues to cultivate 1 month, cut band bud callus and put into propagation cultivation, control cultivation temperature is 24-28 ℃, illumination is that 70-100 μ mol/ms breeds cultivation, and indefinite bud growth there is no rapidly vitrified bad phenomenon;
(3) indefinite bud strong seedling culture
Every clump of the Multiple Buds inducing has 2-3 strain to extend, and all the other are divided into after little Cong or simple bud in dwarfing state, put into strong seedling culture base, control cultivation temperature is 24-28 ℃, and illumination is that 70-100 μ mol/ms carries out strong seedling culture, indefinite bud extends rapidly, after 20 days, can grow to 2-3cm;
(4) culture of rootage
Get the plantlet of 2-3cm, transfer in root media, control cultivation temperature is 24-28 ℃, and illumination is that 70-100 μ mol/ms carries out root induction, and after 10 days, seedling base section dissolves the former base of root of many whites, after 30 days, grows to 3-4cm;
(5) hardening and transplanting
Culture of rootage 20-30 days, root system grows to 1-2cm, selects the aseptic seedling of well developed root system robust growth, and indoor uncork hardening 5 days is taken out afterwash root agar, tames after 40 days in greenhouse, transplants outdoor.
The basic ingredient of described bud inducing culture is sucrose 20-40g/L, agar 3-8g/L, and nutrient component is MS+6-BA1.0-5.0mg/L+NAA0.1-0.5mg/L, the pH value of bud inducing culture is 5.5-6.
The preferred MS+6-BA3mg/L+NAA0.3mg/L of nutrient component of described bud inducing culture.
The basic ingredient of described proliferated culture medium is sucrose 20-40g/L, agar 3-8g/L, and nutrient component is MS+6-BA1.0-3.0mg/L+NAA0.1-0.3mg/L, the pH value of proliferated culture medium is 5.5-6.
The preferred MS+6BA1mg/L+NAA0.1mg/L of nutrient component of described proliferated culture medium.
The basic ingredient of described strong seedling culture base is sucrose 20-40g/L, agar 3-8g/L, and nutrient component is MS+6-BA0.5-2.0mg/L+NAA0.1-0.2mg/L, the pH value of strong seedling culture base is 5.5-6.
The preferred MS+6-BA0.5mg/L+NAA0.1mg/L of nutrient component of described strong seedling culture base.
The basic ingredient of described root media is sucrose 20-40g/L, agar 3-8g/L, and nutrient component is MS+NAA0.1-0.5mg/L+IBA1.0-5.0mg/L, the pH value of root media is 5.5-6.
The preferred MS+NAA0.3mg/L+IBA3.0mg/L of nutrient component of described root media.
Compared with prior art, the present invention passes through tissue culture technique, can improve ripening rate, utilize bud inducing culture, proliferated culture medium, strong seedling culture base, the root media selected to provide different nutrient components at different cultivation stages, thereby keep to the full extent original maternal character, and transplanting survival rate in the time of hardening and transplant step can reach 90%.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
Organize a method of cultivating long fringe torch flower, adopt following steps:
(1) bud induction is cultivated: win long fringe torch flower budlet, rinse after 2h on superclean bench with running water, with 75% alcohol immersion 30s, 1 ‰ mercuric chloride soak 15min, aseptic water washing 5-6 time, aseptic filter paper blots surperficial moisture content, get tender shoots base portion and be cut into 1cm length, be inoculated on bud inducing culture, the basic ingredient of this medium is sucrose 20g/L, agar 3g/L, nutrient component is MS+6-BA1.0mg/L+NAA0.1mg/L, and pH value is 5.5, and controlling cultivation temperature is 24 ℃, illumination is 100 μ mol/ms, carries out bud induction and cultivates;
(2) bud differentiation and proliferation: after tender shoots is inoculated on bud inducing culture 5 weeks, bastem portion starts to expand, there is yellow green projection, visible significantly callus after 3 weeks, cultivate 1 month, cut band bud callus and put into propagation cultivation, the basic ingredient of this medium is sucrose 20g/L, agar 3g/L, nutrient component is MS+6-BA1mg/L-+NAA0.1mg/L, pH value is 5.5, controlling cultivation temperature is 24 ℃, illumination is 100 μ mol/ms, breed cultivation, base portion callus is many, but do not affect the propagation of indefinite bud, indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, in this cultivation, grow good,
(3) indefinite bud strong seedling culture: every clump of the Multiple Buds inducing only has 2-3 strain to extend, all the other are divided into after little Cong or simple bud in dwarfing state, put into strong seedling culture base, the basic ingredient of this medium is sucrose 20g/L, agar 3g/L, nutrient component is MS+6-BA0.5mg/L+NAA0.1mg/L, pH value is 5.5, controlling cultivation temperature is 24 ℃, illumination is 100 μ mol/ms, and indefinite bud extends rapidly, after 20 days, can grow to 2-3cm;
(4) culture of rootage: get the plantlet of 2-3cm, transfer in root media, the basic ingredient of this medium is sucrose 20g/L, agar 3g/L, nutrient component is MS+NAA0.1mg/L+IBA1.0mg/L, and controlling cultivation temperature is 24 ℃, and illumination is that 100 μ mol/ms carry out root induction.After 10 days, seedling base section dissolves the former base of root of many whites, after 30 days, can grow to 3-4cm;
(5) hardening and transplanting: culture of rootage is about 20 days, when root system grows to 1-2cm, select the aseptic seedling of well developed root system robust growth, indoor uncork hardening 5 days, take out afterwash root agar, in greenhouse, tame after 40 days, can transplant outdoor, give rich water quality management, transplanting survival rate is 90%.
Embodiment 2
Organize a method of cultivating long fringe torch flower, adopt following steps:
(1) bud induction is cultivated: win long fringe torch flower budlet, rinse after 2h on superclean bench with running water, with 75% alcohol immersion 30s, 1 ‰ mercuric chloride soak 15min, aseptic water washing 5-6 time, aseptic filter paper blots surperficial moisture content, get tender shoots base portion and be cut into 1cm length, be inoculated on bud inducing culture, the basic ingredient of this medium is sucrose 25g/L, agar 4g/L, nutrient component is MS+6-BA2.0mg/L+NAA0.2mg/L, and pH value is 5.7, and controlling cultivation temperature is 25 ℃, illumination is 90 μ mol/ms, carries out bud induction and cultivates:
(2) bud differentiation and proliferation: after tender shoots is inoculated on bud inducing culture 5 weeks, bastem portion starts to expand, there is yellow green projection, visible significantly callus after 3 weeks, cultivate 1 month, cut band bud callus and put into propagation cultivation, the basic ingredient of this medium is sucrose 25g/L, agar 4g/L, nutrient component is MS+6-BA2mg/L-+NAA0.2mg/L, pH value is 5.7, controlling cultivation temperature is 25 ℃, illumination is 90 μ mol/ms, breed cultivation, base portion callus is many, but do not affect the propagation of indefinite bud, indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, in this cultivation, grow good,
(3) indefinite bud strong seedling culture: every clump of the Multiple Buds inducing only has 2-3 strain to extend, all the other are divided into after little Cong or simple bud in dwarfing state, put into strong seedling culture base, the basic ingredient of this medium is sucrose 25g/L, agar 4g/L, nutrient component is MS+6-BA1mg/L+NAA0.1mg/L, pH value is 5.7, control cultivation temperature be 25 ℃ (, illumination is 90 μ mol/ms, and indefinite bud extends rapidly, after 20 days, can grow to 2-3cm;
(4) culture of rootage: get the plantlet of 2-3cm, transfer in root media, the basic ingredient of this medium is sucrose 25g/L, agar 4g/L, nutrient component is MS+NAA0.2mg/L+IBA2.0mg/L, and controlling cultivation temperature is 24 ℃, and illumination is that 100 μ mol/ms carry out root induction.After 10 days, seedling base section dissolves the former base of root of many whites, after 30 days, can grow to 3-4cm;
(5) hardening and transplanting: culture of rootage is about 25 days, when root system grows to 1-2cm, select the aseptic seedling of well developed root system robust growth, indoor uncork hardening 5 days, take out afterwash root agar, in greenhouse, tame after 40 days, can transplant outdoor, give rich water quality management, transplanting survival rate is 90%.
Embodiment 3
Organize a method of cultivating long fringe torch flower, adopt following steps:
(1) bud induction is cultivated: win long fringe torch flower budlet, rinse after 2h on superclean bench with running water, with 75% alcohol immersion 30s, 1 ‰ mercuric chloride soak 15min, aseptic water washing 5-6 time, aseptic filter paper blots surperficial moisture content, get tender shoots base portion and be cut into 1cm length, be inoculated on bud inducing culture, the basic ingredient of this medium is sucrose 30g/L, agar 6g/L, nutrient component is MS+6-BA3.0mg/L+NAA0.3mg/L, and pH value is 5.8, and controlling cultivation temperature is 25 ℃, illumination is 80 μ mol/ms, carries out bud induction and cultivates;
(2) bud differentiation and proliferation: after tender shoots is inoculated on bud inducing culture 5 weeks, bastem portion starts to expand, there is yellow green projection, visible significantly callus after 3 weeks, cultivate 1 month, cut band bud callus and put into propagation cultivation, the basic ingredient of this medium is sucrose 30g/L, agar 6g/L, nutrient component is MS+6-BA2mg/L-+NAA0.2mg/L, pH value is 5.8, controlling cultivation temperature is 25 ℃, illumination is 80 μ mol/ms, breed cultivation, base portion callus is many, but do not affect the propagation of indefinite bud, indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, in this cultivation, grow good,
(3) indefinite bud strong seedling culture: every clump of the Multiple Buds inducing only has 2-3 strain to extend, all the other are divided into after little Cong or simple bud in dwarfing state, put into strong seedling culture base, the basic ingredient of this medium is sucrose 30g/L, agar 6g/L, nutrient component is MS+6-BA1.0mg/L+NAA0.1mg/L, pH value is 5.8, controlling cultivation temperature is 25 ℃, illumination is 80 μ mol/ms, and indefinite bud extends rapidly, after 20 days, can grow to 2-3cm;
(4) culture of rootage: get the plantlet of 2-3cm, transfer in root media, the basic ingredient of this medium is sucrose 30g/L, agar 6g/L, nutrient component is MS+NAA0.3mg/L+IBA3.0mg/L, and controlling cultivation temperature is 25 ℃, and illumination is that 80 μ mol/ms carry out root induction.After 10 days, seedling base section dissolves the former base of root of many whites, can grow to 3-4cm, and root system is sturdy after 30 days, and fibrous root is numerous, and rooting rate is 100%;
(5) hardening and transplanting: culture of rootage is about 25 days, when root system grows to 1-2cm, select the aseptic seedling of well developed root system robust growth, indoor uncork hardening 5 days, take out afterwash root agar, in greenhouse, tame after 40 days, can transplant outdoor, give rich water quality management, transplanting survival rate is 90%.
Embodiment 4
Organize a method of cultivating long fringe torch flower, adopt following steps:
(1) bud induction is cultivated: win long fringe torch flower budlet, rinse after 2h on superclean bench with running water, with 75% alcohol immersion 30s, 1 ‰ mercuric chloride soak 15min, aseptic water washing 5-6 time, aseptic filter paper blots surperficial moisture content, get tender shoots base portion and be cut into 1cm length, be inoculated on bud inducing culture, the basic ingredient of this medium is sucrose 40g/L, agar 8g/L, nutrient component is MS+6-BA5.0mg/L+NAA0.5mg/L, and pH value is 6, and controlling cultivation temperature is 28 ℃, illumination is 70 μ mol/ms, carries out bud induction and cultivates;
(2) bud differentiation and proliferation: after tender shoots is inoculated on bud inducing culture 5 weeks, bastem portion starts to expand, there is yellow green projection, visible significantly callus after 3 weeks, cultivate 1 month, cut band bud callus and put into propagation cultivation, the basic ingredient of this medium is sucrose 40g/L, agar 8g/L, nutrient component is MS+6-BA3mg/L-+NAA0.3mg/L, pH value is 6, controlling cultivation temperature is 28 ℃, illumination is 70 μ mol/ms, breed cultivation, base portion callus is many, but do not affect the propagation of indefinite bud, indefinite bud growth there is no rapidly the bad phenomenon such as vitrifying, in this cultivation, grow good,
(3) indefinite bud strong seedling culture: every clump of the Multiple Buds inducing only has 2-3 strain to extend, all the other are divided into after little Cong or simple bud in dwarfing state, put into strong seedling culture base, the basic ingredient of this medium is sucrose 40g/L, agar 8g/L, nutrient component is MS+6-BA2.0mg/L+NAA0.2mg/L, pH value is 5.5, controlling cultivation temperature is 28 ℃, illumination is 70 μ mol/ms, and indefinite bud extends rapidly, after 20 days, can grow to 2-3cm;
(4) culture of rootage: get the plantlet of 2-3cm, transfer in root media, the basic ingredient of this medium is 40g/L, agar 8g/L, nutrient component is MS+NAA0.5mg/L+IBA5.0mg/L, and controlling cultivation temperature is 28 ℃, and illumination is that 70 μ mol/ms carry out root induction.After 10 days, seedling base section dissolves the former base of root of many whites, after 30 days, can grow to 3-4cm;
(5) hardening and transplanting: culture of rootage is about 30 days, when root system grows to 1-2cm, select the aseptic seedling of well developed root system robust growth, indoor uncork hardening 5 days, take out afterwash root agar, in greenhouse, tame after 40 days, can transplant outdoor, give rich water quality management, transplanting survival rate is 90%.
Claims (9)
1. organize a method of cultivating long fringe torch flower, it is characterized in that, the method adopts following steps:
(1) bud induction is cultivated
Win long fringe torch flower budlet, after cleaning, 2h soaks 15min, aseptic water washing 5-6 time with alcohol immersion 30s, 1wt ‰ mercuric chloride of 75v/v%, then blot surperficial moisture content, get tender shoots base portion and be cut into 1cm length, be inoculated on bud inducing culture, control cultivation temperature is 24-28 ℃, and illumination is 70-100 μ mol/ms, carries out bud induction and cultivates;
(2) bud differentiation and proliferation
After tender shoots is inoculated on bud inducing culture 5 weeks, bastem portion starts to expand and occurs yellow green projection, after 3 weeks, visible significantly callus continues to cultivate 1 month, cut band bud callus and put into propagation cultivation, control cultivation temperature is 24-28 ℃, illumination is that 70-100 μ mol/ms breeds cultivation, and indefinite bud growth there is no rapidly vitrified bad phenomenon;
3) indefinite bud strong seedling culture
Every clump of the Multiple Buds inducing has 2-3 strain to extend, and all the other are divided into after little Cong or simple bud in dwarfing state, put into strong seedling culture base, control cultivation temperature is 24-28 ℃, and illumination is that 70-100 μ mol/ms carries out strong seedling culture, indefinite bud extends rapidly, after 20 days, can grow to 2-3cm;
4) culture of rootage
Get the plantlet of 2-3cm, transfer in root media, control cultivation temperature is 24-28 ℃, and illumination is that 70-100 μ mol/ms carries out root induction, and after 10 days, seedling base section dissolves the former base of root of many whites, after 30 days, grows to 3-4cm;
(5) hardening and transplanting
Culture of rootage 20-30 days, root system grows to 1-2cm, selects the aseptic seedling of well developed root system robust growth, and indoor uncork hardening 5 days is taken out afterwash root agar, tames after 40 days in greenhouse, transplants outdoor.
2. a kind of method of cultivating long fringe torch flower of organizing according to claim 1, it is characterized in that, the basic ingredient of described bud inducing culture is sucrose 20-40g/L, agar 3-8g/L, nutrient component is MS+6-BA1.0-5.0mg/L+NAA0.1-0.5mg/L, and the pH value of bud inducing culture is 5.5-6.
3. a kind of method of cultivating long fringe torch flower of organizing according to claim 2, is characterized in that the preferred MS+6-BA3mg/L+NAA0.3mg/L of nutrient component of described bud inducing culture.
4. a kind of method of cultivating long fringe torch flower of organizing according to claim 1, it is characterized in that, the basic ingredient of described proliferated culture medium is sucrose 20-40g/L, agar 3-8g/L, nutrient component is MS+6-BA1.0-3.0mg/L+NAA0.1-0.3mg/L, and the pH value of proliferated culture medium is 5.5-6.
5. a kind of method of cultivating long fringe torch flower of organizing according to claim 4, is characterized in that the preferred MS+6BA1mg/L+NAA0.1mg/L of nutrient component of described proliferated culture medium.
6. a kind of method of cultivating long fringe torch flower of organizing according to claim 1, it is characterized in that, the basic ingredient of described strong seedling culture base is sucrose 20-40g/L, agar 3-8g/L, nutrient component is MS+6-BA0.5-2.0mg/L+NAA0.1-0.2mg/L, and the pH value of strong seedling culture base is 5.5-6.
7. a kind of method of cultivating long fringe torch flower of organizing according to claim 6, is characterized in that the preferred MS+6-BA0.5mg/L+NAA0.1mg/L of nutrient component of described strong seedling culture base.
8. a kind of method of cultivating long fringe torch flower of organizing according to claim 1, it is characterized in that, the basic ingredient of described root media is sucrose 20-40g/L, agar 3-8g/L, nutrient component is MS+NAA0.1-0.5mg/L+IBA1.0-5.0mg/L, and the pH value of root media is 5.5-6.
9. a kind of method of cultivating long fringe torch flower of organizing according to claim 8, is characterized in that the preferred MS+NAA0.3mg/L+IBA3.0mg/L of nutrient component of described root media.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869061A (en) * | 2009-04-24 | 2010-10-27 | 上海上房园林植物研究所 | Tissue culture method of ophiopogon planiscapus |
| CN101869062A (en) * | 2009-04-24 | 2010-10-27 | 上海上房园林植物研究所 | Tissue culture method of Hemerocallis dumortieri |
| CN102265785A (en) * | 2010-06-02 | 2011-12-07 | 上海上房园艺有限公司 | Tissue culturing method of hemerocallis middendorfii poinsettia |
| CN102273405A (en) * | 2010-06-10 | 2011-12-14 | 上海上房园艺有限公司 | Cultivating method of Hosta plantaginea Gold Standard |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869061A (en) * | 2009-04-24 | 2010-10-27 | 上海上房园林植物研究所 | Tissue culture method of ophiopogon planiscapus |
| CN101869062A (en) * | 2009-04-24 | 2010-10-27 | 上海上房园林植物研究所 | Tissue culture method of Hemerocallis dumortieri |
| CN102265785A (en) * | 2010-06-02 | 2011-12-07 | 上海上房园艺有限公司 | Tissue culturing method of hemerocallis middendorfii poinsettia |
| CN102273405A (en) * | 2010-06-10 | 2011-12-14 | 上海上房园艺有限公司 | Cultivating method of Hosta plantaginea Gold Standard |
Non-Patent Citations (1)
| Title |
|---|
| 谭文澄等: "火炬花的组织培养和植株再生", 《植物生理学通讯》 * |
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