CN103752594B - A kind of plant restoration method of phthalic acid ester contaminated soil - Google Patents
A kind of plant restoration method of phthalic acid ester contaminated soil Download PDFInfo
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- CN103752594B CN103752594B CN201310745346.XA CN201310745346A CN103752594B CN 103752594 B CN103752594 B CN 103752594B CN 201310745346 A CN201310745346 A CN 201310745346A CN 103752594 B CN103752594 B CN 103752594B
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Abstract
The invention belongs to the recovery technique field of contaminated soil, disclose a kind of plant restoration method of phthalic acid ester contaminated soil. The method comprises the following steps: (1) is smooth by soil breaking, bag Gou Qi Kui, and use base manure, water waters to ground moistening; (2) before sowing, every cave packs Nutrition Soil or culture medium into, trickle, and seeding corn seed, then cover Nutrition Soil; (3) balled transplanting when seedling grows to 3～4 true leaves, keeps ground moistening; (4) corn grew to before the fringe phase, harvesting corn plant. The present invention, by utilizing maize root system to combine with rhizosphere microorganism, promotes phthalic acid ester degraded, makes clearance in its soil reach 86%, realizes the efficient Phthalate Esters In Soil of removing. After reaping corn, cauline leaf can be used as feed, has economic benefit. Corn growth is fast, well developed root system, and biomass is large, can effectively remove Phthalate Esters In Soil; Be applicable in phthalic acid ester on a large scale, the reparation of low concentration pollution soil.
Description
Technical field
The invention belongs to the recovery technique field of contaminated soil, particularly a kind of phthalic acid ester Polluted SoilThe plant restoration method of earth.
Background technology
Phthalic acid ester (PAEs, claims again phthalate ester, is commonly called as plasticiser) is important the organising of a classCompound, they are widely used as plastic plasticizer, farm chemical carrier, pest repellant, clothes, cosmetics, lubricatedThe raw materials for production of agent etc. Wherein consumption maximum is plastic plasticizer, accounts for 80% of its total output. PAEsAlso be a class environment incretion interferent, what have has a Reproductive and developmental toxicity, hepatotoxicity and potential carcinogenicProperty and bioaccumulation effect, wherein 6 kinds of PAEs compounds are by China Environmental Monitoring General Station, American NationalEnvironmental Protection Agency and European Union etc. classify " excellent control pollutant " as.
In recent years, along with a large amount of uses and the covering with ground sheeting (comprise hot house) of PAEs in industry plantedThe extensive use of training technology, the pollution of PAEs general (Cai Quanying etc., 2005 in agricultural soil; Yang GuoyiDeng, 2007; Caietal., 2008; Xuetal., 2008; Zengetal., 2008,2009; Xiaetal.,2011.). China's industrial or agricultural district soil is affected by PAEs all in various degree, and content is generally microgram/thousandGram to the mg/kg order of magnitude, comparatively serious with dirty irrigated area and industrial area soil pollution. Considerable part soilTwo (2-ethylhexyl) esters (DEHP) of middle phthalic acid and the positive dibutyl ester of phthalic acid (DnBP)Deng the relevant control standard (Cai Quanying etc., 2005 that have exceeded Soil Loss; Caietal., 2008.). And,The content of PAEs in soil is far above the persistence organic pollutant such as polycyclic aromatic hydrocarbon, Polychlorinated biphenyls (CaietAl., 2008.), become the global important environmental organic pollutant of a class at present. Hydrophobic due to PAEsProperty and anti-degradation capability are strong, easily cause its enrichment in environment, and this certainly will have influence on plants in this pollutionThe safety of crops on soil, harm humans healthy.
The recovery technique of the organic polluted soil such as heavy metal and polycyclic aromatic hydrocarbon is carried out to large quantity research both at home and abroad,But relative with recovery technique research little to the improvement of PAEs contaminated soil. General contaminated soil can adoptPhysical chemistry is repaired and the large class methods of biological restoration two. Physical chemistry reparation comprise soil moved in to improve the original method, chemistry fixing,Electronic reparation, Soil leaching etc., these technology not only very expensive, be difficult to extensive improvement, and can causeDegradation under soil texture destruction and fertility. At present, be mainly to adopt biology to PAEs contaminated soil both at home and abroadRestorative procedure, especially microorganism are repaired. Be mainly by screening microbiological bacterials, utilize efficient degradationPAEs bacterial classification removes PAEs in soil. For example, Qin Hua etc. screens the micro-life to DEHP efficient degradationThing bacterial strain bacillus (Bacillussp1DW1) and leather are stepped on Salmonella (GordonaspDH3). Duan XingchunBe separated to the not lever of calcium acetate of the dibutyl phthalate (DBP) of degrading Deng domestication from contaminated soilPseudomonas (Acinetobactercalcoaceticus) and pseudomonas aeruginosa (Pseudomonasaeruginosa),Degradation rate to 40mg/L initial concentration DBP reaches respectively 98.64%, 74.62%. Microorganism is repairedRelate to the screening of efficient degrading bacteria, and by such inoculation in PAEs contaminated soil, this technology weekPhase is relatively long, and degradation bacteria is subject to the impacts such as a lot of environmental factors after being inoculated in soil and is difficult to reach realTest the removal effect in chamber.
Phytoremediation refers to that the system of coexisting of utilizing plant and rhizosphere microorganism thereof absorbs, holds, transforms or turnsMoving pollutant makes it harmless. Be mainly by planting plants impurely, realize partially or completely and repair and polluteThe original position of environment is administered technology. Both at home and abroad the phytoremediation of heavy metal and polycyclic aromatic hydrocarbon pollution is entered at presentGo large quantity research, but utilized the rarely seen report of method of phytoremediation PAEs contaminated soil.
Summary of the invention
In order to overcome the shortcoming and deficiency of above-mentioned prior art, it is a kind of adjacent that primary and foremost purpose of the present invention is to provideThe plant restoration method of phthalic acid ester contaminated soil, degraded phthalic acid ester, recovery soil.
Object of the present invention realizes by following proposal:
A plant restoration method for phthalic acid ester contaminated soil, comprises the following steps:
(1) soil breaking is smooth, bag Gou Qi Kui, and use base manure, water waters to ground moistening;
(2) before sowing, every cave packs Nutrition Soil or culture medium into, trickle, and seeding corn seed, then cover battalionSupport soil;
(3) balled transplanting when seedling grows to 3～4 true leaves, keeps ground moistening;
(4) corn grew to before the fringe phase, harvesting corn plant.
The present invention, by utilizing maize root system to combine with rhizosphere microorganism, promotes phthalic acid ester degraded, makesThe clearance of soil DEHP reaches 86% left and right, reaches efficient removal Phthalate Esters In Soil.
After reaping corn, can be used as feed. If whether the content of phthalic acid ester in uncertain milpaToo high or ultra mark, phthalic acid ester content in the recorded plant body of conditions permit, is used further to prepare feed.
The plant spacing in the rows of transplanting described in step (3) is preferably 10～12cm.
When harvesting corn plant described in step (4) is preferably corn growth 40～45 days.
The concentration of step (1) Phthalate Esters In Soil is 100mg/Kg or following.
Described phthalic acid ester is two (2-ethylhexyl) esters (DEHP) of phthalic acid and adjacent benzeneAt least one in the positive dibutyl ester of dioctyl phthalate (DnBP).
Preferably, described phthalic acid ester is two (2-ethylhexyl) esters (DEHP) of phthalic acid.
The described corn seed of step (2) is preferably ten thousand blue or green kinds.
Preferably, described corn seed refers to selected evenly full corn seed.
Preferably, before described corn seed sowing, clean with 0.1% mercury chloride sterilization.
Preferably, the special seedlings cultivating board of 100 cave corn is selected in described sowing, and every mu with coiling 40 left and right.
Preferably, the amount that adds Nutrition Soil or culture medium described in is 2/3 of cave inner volume.
Preferably, described trickle refers to drench water to water capacity 70% left and right.
Preferably, in above-mentioned planting process, do not use agricultural chemicals as far as possible.
The present invention has following advantage and beneficial effect with respect to prior art:
(1) corn growth is fast, well developed root system, and biomass is large, effectively removes Phthalate Esters In Soil;
(2) corn strong adaptability, is suitable for being planted on different soils;
(3) maize root system is combined with rhizosphere microorganism, can promote phthalic acid ester degraded, makes in soilThe clearance of phthalic acid ester reaches 86%, realizes the efficient phthalic acid ester of removing.
(4) in the preferred ten thousand blue or green kind corn stem leafs of the present invention, phthalic acid ester absorption and accumulation amount is obviously lowIn other kinds and the root system of self, can effectively avoid the residual problem exceeding standard of phthalic acid ester, therefore receiveThe corn stem leaf cutting can be used as feed, has economic benefit.
(5) the inventive method still can under up to 117mg/Kg condition in Phthalate Esters In Soil concentrationRealize 86% clearance, be therefore applicable in phthalic acid ester on a large scale, the repairing of low concentration pollution soilMultiple.
Brief description of the drawings
Fig. 1 is the biomass of different cultivars corn.
Fig. 2 is DEHP content in the cauline leaf of different cultivars corn and root system.
Fig. 3 is the residual concentration of DEHP in soil after plantation different cultivars corn.
Fig. 4 is the clearance of soil DEHP after plantation different cultivars corn.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but enforcement side of the present inventionFormula is not limited to this.
Embodiment 1: in indoor pot experiment of carrying out
(1) rice soil air-dry, pulverize after be mixed with DEHP contaminated soil.
(2) DEHP contaminated soil is put into porcelain basin, lower end apopore is blocked with window screening and broken ceramics,The native 4Kg of every basin, in soil, the initial concentration of DEHP is 117mg/Kg.
(3) water to ground moistening with running water, after antecedent soil moisture, (dress basin after the 5th day) poured out brokenlyAfter broken mixing, refill basin.
(4) totally 8 kinds of the corn varieties of plantation, comprise ten thousand green grass or young crops, Jin Hai-No. 6, zea mexicana, Hua Nong-No. 1, Huahai, can single-No. 4, white jade glutinous and super sweet-23. By selected evenly full corn seed,Adopt 0.1% mercury chloride sterilization to clean.
(5) seeding and seedling raising: adopting river sand is seedling medium, and seedling medium is put into seedlings cultivating board, paves,Then the corn seed after sterilization is evenly sown in the sand of river, then with the covering of a small amount of rice soil. Water waters pouring,Sooner or later water once every day later.
(6), in the time that seedling grows to 3～4 true leaves, transplant seedlings field planting to DEHP Polluted Soil is housed from seedlings cultivating boardIn the porcelain basin of earth, the plant spacing in the rows of corn is 10～12cm. Sooner or later water once every day. Corn planting mistakeIn journey, do not use agricultural chemicals.
(7) when corn grows to 40～45 days, harvesting corn plant, point overground part (being cauline leaf) and undergroundPortion's (root system) gathers, and gathers pedotheque simultaneously. Measure the biomass (Fig. 1) of milpa.
(8) after harvesting corn, record respectively the residual dense of DEHP in corn stem leaf, root system and pedothequeDegree (Fig. 2 and Fig. 3). According to the initial concentration of soil, calculate the clearance (Fig. 4) of DEHP in soil.
By finding out in Fig. 1, corn plant biomass is at 103.48～144.39g/ basin, wherein with white jadeGlutinous biomass is for the highest, Hua Nong-No. 1, ten thousand blue or green and super sweet-23 biomass is also higher.
Fig. 2 has provided DEHP content in the cauline leaf of corn and root system. As seen from the figure, corn stem leafThe content of middle DEHP is at 0.74～2.46mg/Kg, wherein the highest with super sweet-23 cauline leafs, ten thousand sapphire riceMinimum, and significant difference (except zea mexicana) between other processing. Ten thousand blue or green corn stem leafs pair are describedThe absorption cumulant of DEHP is relatively low, even lower than being planted in logical dish on DEHP contaminated soil (againName water spinach) also low (Cai Quanying etc., 2004.) of content; Far below being grown near farmland plastics industry districtThe concentration (Wang Jiawen etc., 2010.) of vegetables DEHP. Not yet formulate both at home and abroad at present in plant feedThe limit standard of DEHP. The DEHP amount of taking in from food the every day per capita of Environmental Protection Agency's regulation is not superCross 0.25mg(Cai all over Britain etc., 2004.). If corn is as feed, animal permission is taken in from foodDEHP amount should be slightly higher than 0.25mg. By the result of present case, the possibility comparison that ten thousand sapphire rice exceed standardLittle, therefore belong to most preferably kind.
Fig. 3 has provided after maize planting the residual concentration of DEHP in soil. As seen from Figure 3, plantPlant after different cultivars corn in soil the residual concentration difference of DEHP larger, with Huahai's corn varietyHeight, Wan Qing, Hua Nong-No. 1 and glutinous lower of white jade, significant difference between processing.
Fig. 4 has provided after maize planting the clearance of DEHP in soil. Contrary with residual concentration, Wan Qing,The DEHP clearance of Hua Nong-No. 1 and the glutinous processing of white jade is higher. In conjunction with DEHP's in Fig. 2 corn stem leafAbsorb cumulative concentration, can find out that the absorption cumulant of ten thousand sapphire rice is lower, but clearance is higher, illustrates ten thousandSapphire rice can promote the degraded of soil DEHP, and the biomass of ten thousand sapphire rice is relatively high, therefore,Ten thousand sapphire rice are larger to the reparative potential of soil DEHP, can ensure better the safety of corn, are used asFeed, has certain economic benefit.
Above-described embodiment is preferably embodiment of the present invention, but embodiments of the present invention are not subject to above-mentioned realityExecute routine restriction, other any do not deviate from the change done under Spirit Essence of the present invention and principle, modification,Substitute, combine, simplify, all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (9)
1. a plant restoration method for phthalic acid ester contaminated soil, is characterized in that comprising following stepRapid:
(1) soil breaking is smooth, bag ditch plays furrow, and uses base manure, and water waters to ground moistening;
(2) before sowing, every cave packs Nutrition Soil into, trickle, and seeding corn seed, then cover Nutrition Soil;
(3) balled transplanting when seedling grows to 3～4 true leaves, keeps ground moistening;
(4) corn grew to before the fringe phase, harvesting corn plant;
The described corn seed of step (2) is ten thousand blue or green kinds.
2. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: the plant spacing in the rows of transplanting described in step (3) is 10～12 centimetres.
3. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: when harvesting corn plant described in step (4) is corn growth 40～45 days.
4. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: the concentration of the described Phthalate Esters In Soil of step (1) is 100 mg/kg or is less than 100Mg/kg.
5. the plant restoration method of phthalic acid ester contaminated soil according to claim 4, its spyLevy and be: described phthalic acid ester is two (2-ethylhexyl) ester and the phthalic acids of phthalic acidAt least one in positive dibutyl ester.
6. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: before the described corn seed sowing of step (2), clean with 0.1% mercury chloride sterilization.
7. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: the special seedlings cultivating board of 100 cave corn is selected in the described sowing of step (2), every mu with coiling 40.
8. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: described trickle refers to drench water to water capacity 70% left and right.
9. the plant restoration method of phthalic acid ester contaminated soil according to claim 1, its spyLevy and be: after described milpa harvesting, be used as and prepare feed.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105290094B (en) * | 2015-11-25 | 2017-11-28 | 沈阳大学 | The method polluted using phthalate in Vermicomposting degraded plastic shed soil |
| CN106424127B (en) * | 2016-09-05 | 2019-06-18 | 青岛农业大学 | The restorative procedure of phthalic acid ester contaminated soil |
| CN108580542A (en) * | 2018-05-22 | 2018-09-28 | 西南大学 | A kind of method of repefral in photocatalytic degradation contaminated soil |
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| CN101687699A (en) * | 2007-06-18 | 2010-03-31 | 欧洲圣戈班技术结构公司 | Glass fibre structure for reinforcing agglomerated abrasive objects |
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| CN101687699A (en) * | 2007-06-18 | 2010-03-31 | 欧洲圣戈班技术结构公司 | Glass fibre structure for reinforcing agglomerated abrasive objects |
| CN102477403A (en) * | 2010-11-23 | 2012-05-30 | 中国科学院生态环境研究中心 | Degradation bacteria of phthalate compounds and production method of degradation bacteria |
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