CN103743896A - Detachable, volume adjustable and evaporation-prevention glass slide incubator - Google Patents
Detachable, volume adjustable and evaporation-prevention glass slide incubator Download PDFInfo
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- CN103743896A CN103743896A CN201410012875.3A CN201410012875A CN103743896A CN 103743896 A CN103743896 A CN 103743896A CN 201410012875 A CN201410012875 A CN 201410012875A CN 103743896 A CN103743896 A CN 103743896A
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- 239000011521 glass Substances 0.000 title abstract 7
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 35
- 239000010935 stainless steel Substances 0.000 claims abstract description 35
- 238000001704 evaporation Methods 0.000 claims abstract description 11
- 230000012447 hatching Effects 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 24
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 20
- 239000000741 silica gel Substances 0.000 claims description 20
- 229910002027 silica gel Inorganic materials 0.000 claims description 20
- 229960001866 silicon dioxide Drugs 0.000 claims description 20
- 230000002265 prevention Effects 0.000 claims description 14
- 238000009834 vaporization Methods 0.000 claims description 14
- 230000008016 vaporization Effects 0.000 claims description 14
- 230000008020 evaporation Effects 0.000 claims description 10
- 230000002093 peripheral effect Effects 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 7
- 238000013508 migration Methods 0.000 claims description 5
- 230000005012 migration Effects 0.000 claims description 5
- 241000397426 Centroberyx lineatus Species 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 238000011534 incubation Methods 0.000 abstract description 31
- 239000007788 liquid Substances 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 abstract description 7
- 238000011160 research Methods 0.000 abstract description 3
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract 2
- 229910052710 silicon Inorganic materials 0.000 abstract 2
- 239000010703 silicon Substances 0.000 abstract 2
- 230000008569 process Effects 0.000 description 6
- 230000018044 dehydration Effects 0.000 description 5
- 238000006297 dehydration reaction Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000011532 immunohistochemical staining Methods 0.000 description 4
- 238000003364 immunohistochemistry Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000002055 immunohistochemical effect Effects 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
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Abstract
The invention discloses a detachable, volume adjustable and evaporation-prevention glass slide incubator. A stainless steel incubation frame is pressed on a glass slide, and forms a small incubation chamber by a rectangular space in the middle by taking the glass slide as the bottom surface together with the silicon gel cushion layer below; elastic clampers which clamp the stainless steel incubation frame and the silicon gel cushion layer on the glass slide are arranged additionally; four surfaces of the inner walls of the stainless steel incubation frame and the gel cushion layer are respectively provided with a dovetail groove; the dovetail grooves and incubation chamber space regulating blocks with dovetail-shaped bulges are inserted and matched; an upper cover plate and an upper cover handle of the incubation chamber are arranged additionally. The detachable, volume adjustable and evaporation-prevention glass slide incubator provided by the invention is reasonable in structure, guarantees the effect that when an antibody is incubated, all tissue slices pasted on the glass slide can be completely dipped into an antibody incubation liquid by utilizing trace antibody incubation liquid, prevents moisture in the antibody incubation liquid from evaporating, and further saves the experiment cost of scientific research.
Description
Technical field
The present invention relates to the microslide couveuse of a kind of detachable, volume adjustable and vaporization prevention.
Background technology
Carrying out in the research of life science, people often need to use high specificity, highly sensitive immunohistochemistry technology to position histiocytic predetermined substance and quantitatively.The ultimate principle of immunohistochemistry technology is first to utilize immunologic known antibodies (first antibody) to need to identify antigen in observed tissue to be combined.Because the association reaction of the antigen-antibody carrying out on histocyte is generally sightless, need to by the method for mark, certain label (as enzyme, fluorescein) be connected on first antibody again to the fluorescence that then shows this label or send at the micro-Microscopic observation fluorescein of fluorescent microscope, lazer scan confocal microscope or ultrahigh resolution with histochemical method.Make the Cucumber in observed histocyte visual.When stating in realization the method with mark of mentioning certain label (as enzyme, fluorescein) being attached on first antibody, people often adopt the method in conjunction with the second antibody of upper certain label and the specific binding of first antibody.
Histiocytic predetermined substance being positioned with immunohistochemistry technology and quantitatively time, people adopt the method for paster dyeing to keep testing the good form of histotomy and the front and back of clearly each histotomy sequentially conventionally.The ultimate principle of the method for paster dyeing is on microtome, to cut after tissue, immediately this histotomy is sticked on microslide by section order.Sealing, the first antibody of follow-up histotomy hatched, washing, second antibody are hatched, wash again, dehydration, transparent and mounting are all to stick under the condition on microslide and carry out at this histotomy.
In sealing, the washing of above-mentioned histotomy, wash again, in dehydration, transparent and mounting process, because the reagent cost of these solution of preparation is lower, in addition in washing process, the large usage quantity of cleansing solution.In order to obtain good experiment effect, people are conventionally placed on the whole microslide that is pasted with histotomy in above-mentioned related solution and process.But carrying out first antibody and second antibody while hatching, because antibody price is higher, (price of at present general monoclonal first antibody 100 μ l is 3000 yuan, special monoclonal first antibody price can be higher), and use time attenuable multiple again can not be very large.Therefore, antibody especially first antibody become the major part of immunohistochemical assay cost.So histiocytic predetermined substance being positioned with immunohistochemistry technology and quantitatively time, can not complete by the method that the whole microslide that is pasted with histotomy is placed in antibody incubation liquid.
People, when paster method carries out immunohistochemical staining, conventionally adopt antibody incubation liquid are directly added in to the method on the histotomy of microslide.But while using this method, antibody incubation liquid has been added can preserve and diffuse out microslide loss, add lacked the antibody incubation liquid on histotomy can be in the process of hatching, because moisture evaporation in Incubating Solution can not keep the histotomy on microslide to be immersed in all the time in antibody incubation liquid.Sometimes, people also can use the method for drawing a frame around the histotomy of microslide attaching with oily material, and add antibody incubation liquid in this oiliness picture frame, prevent overflowing of antibody incubation liquid.Though but the method than by antibody incubation liquid be directly added in microslide attach histotomy on method have improvements, but use the method, because oiliness picture frame stops the excessive poor ability of antibody incubation liquid, can not guarantee that histotomy is immersed in antibody incubation liquid all the time in whole antibody incubation process.And in antibody incubation, washing, dehydration, transparent process, these oily materials can lose because coming off the excessive effect of antibody incubation liquid in antibody incubation process that stops; When generation oily material comes off and sticks on histotomy, can affect final sections observation effect; And oily material, also can be molten in relevant experimental solutions in the processing procedure of histotomy, pollute experimental solutions and the recycling of experimental solutions and the treatment effect of histotomy that affect.
Summary of the invention
The object of the present invention is to provide a kind of rational in infrastructurely, all experimental procedure energy convenience and high-efficiencies carry out, and can obtain detachable, the volume adjustable of good immunohistochemical staining experimental result and the microslide couveuse of vaporization prevention.
Technical solution of the present invention is:
The microslide couveuse of a kind of detachable, volume adjustable and vaporization prevention, it is characterized in that: comprise that stainless steel hatches frame, stainless steel is hatched frame and is pressed on microslide, jointly by middle coffin, take microslide, as bottom surface, is formed and hatches cell with the silica gel bed course below it; Described silica gel bed course has with stainless steel hatches the intermediate rectangular space that frame is identical, and sticks to stainless steel and hatch on frame, contacts with microslide, prevents the leakage of Incubating Solution; Stainless steel hatches the peripheral dimension of frame and the peripheral dimension of silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched to frame and silica gel bed course is clamped in the resilient clamp on microslide; Described resilient clamp comprises the clamping part of resilient clamp root, resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and the hand position of resilient clamp when forming microslide couveuse and the operation of dismounting microslide couveuse is provided; Clamp port by resilient clamp is clamped in resilient clamp to hatch on frame and microslide, and the clamping part of resilient clamp will be hatched frame and microslide and is clamped together to form and hatch cell; The inwall four sides that stainless steel is hatched frame and silica gel bed course arranges respectively a dovetail groove, dovetail groove is pegged graft and is coordinated with the camera incubata Space adjustment piece with swallow-tail form convex body, regulate the size in camera incubata space, prevent that camera incubata Space adjustment piece is hatching migration in cell; Separately there is camera incubata upper cover cover plate to cover and hatching frame upper surface, prevent from hatching the evaporation of moisture in Incubating Solution in cell; Camera incubata upper cover handle is set on camera incubata upper cover cover plate, by hand-held this handle, opens or cover camera incubata upper cover.
Using method is: stainless steel is hatched to frame and silicagel pad is laminated on the microslide that is pasted with histotomy, guarantee the bottom of hatching cell that all histotomies are all being hatched frame and microslide and formed.The any one side in frame and upper and lower overlapping four limits of microslide is hatched in selection, hand-held resilient clamp root, the clamp port of resilient clamp is aimed at and hatched the structure that frame and microslide are stacked up and down, resilient clamp is pushed and hatches the structure that frame and microslide are stacked up and down, the clamping part that guarantees resilient clamp will be hatched frame and microslide forcing together tightly, its excess-three limit is repeated above-mentioned action resilient clamp is sandwiched, guarantee to hatch frame and microslide close contact, form the cell of hatching not leaking, form hatching after cell of not leaking, the position attaching on microslide according to histotomy, optionally to hatching, in frame, in 4 dovetail grooves in rectangular wall, insert 1 to 4 camera incubata Space adjustment piece, with flexible, hatch rectangle in frame arbitrarily or the size in polygon space on one side, regulate after the size in camera incubata space, to hatching the Incubating Solution that adds first antibody in cell, assurance sticks on the histotomy of hatching cell bottom and can be immersed in after the Incubating Solution of first antibody, hand-held camera incubata upper cover handle, camera incubata upper cover cover plate is covered at stainless steel and hatches frame upper surface, prevent because of moisture in Incubating Solution evaporation change the concentration of volume and the first antibody of Incubating Solution, then carry out on request hatching of histotomy, after first antibody has been hatched, hand-held camera incubata upper cover handle is opened camera incubata upper cover cover plate, the Incubating Solution of sucking-off first antibody, extract out to insert and hatch the camera incubata Space adjustment piece in rectangular wall dovetail groove in frame, take off and hatch frame and the microslide resilient clamp on phase stack structure four limits up and down, from microslide, unload and hatch frame, the histotomy sticking on microslide is carried out to carrying out washing treatment.Need to carry out second antibody while hatching, repeat above-mentioned action.
The present invention is rational in infrastructure, on the microslide of histotomy, form one do not leak hatch cell; To antibody incubation chamber, insert the method for 1 to 4 camera incubata Space adjustment piece and come flexible antibody incubation chamber arbitrarily or the size in polygon space on one side, remove the unnecessary space of hatching in camera incubata; At stainless steel, hatch frame upper surface and add a cover, prevented the evaporation of moisture in antibody incubation liquid; Final form one hatch space suitable, do not leak and vaporization prevention hatch cell.Guaranteed when carrying out antibody incubation, use more micro-antibody incubation liquid, all histotomies that just can make to stick on microslide can be fully immersed in antibody incubation liquid, and can maintain the stable of antibody incubation liquid composition.Further saved the experimental cost of scientific research.Meanwhile, the impact that by repeatedly taking out camera incubata Space adjustment piece and unloading and hatch frame, guaranteed again microslide pre-treatment, organize paster, the experimental procedure such as washing, dehydration, transparent, mounting can not hatched frame and Space adjustment piece.All experimental procedure energy convenience and high-efficiencies are carried out, and finally can obtain the experimental result of a good immunohistochemical staining.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
Fig. 1 is the camera incubata upper cover schematic perspective view of the microslide couveuse of detachable, volume adjustable and vaporization prevention.
Fig. 2 be detachable, volume adjustable and vaporization prevention microslide couveuse hatch frame schematic perspective view.
Fig. 3 is the microslide schematic perspective view of the microslide couveuse of detachable, volume adjustable and vaporization prevention.
Fig. 4 is the resilient clamp schematic perspective view of the microslide couveuse of detachable, volume adjustable and vaporization prevention.
Fig. 5 is the camera incubata Space adjustment piece schematic perspective view of the microslide couveuse of detachable, volume adjustable and vaporization prevention.
Fig. 6 is the microslide couveuse longitudinal profile schematic diagram of the microslide couveuse of detachable, volume adjustable and vaporization prevention.
In figure:
1. camera incubata upper cover handle: be placed in the upper surface of camera incubata upper cover, open or cover camera incubata upper cover by hand-held this handle.
2. camera incubata upper cover cover plate: cover and hatching frame upper surface, prevent from hatching the evaporation of moisture in Incubating Solution in cell.
3. stainless steel is hatched frame: be pressed on microslide, jointly by middle coffin, take microslide, as bottom surface, formed and hatch cell with the silica gel bed course below it
4. stainless steel is hatched the silica gel bed course of frame: have with stainless steel and hatch the intermediate rectangular space that frame is identical, and stick to stainless steel and hatch on frame, form while hatching cell, contact with microslide, prevent the leakage of Incubating Solution.
5. hatch the dovetail groove in rectangular wall in frame: fixing camera incubata Space adjustment piece, prevents that camera incubata Space adjustment piece is hatching migration in cell.
6. microslide: carrying histotomy, and as the bottom surface of hatching cell.
7. resilient clamp root: connect resilient clamp clamping part, and the handgrip part of resilient clamp when forming microslide couveuse and dismounting microslide couveuse is provided.
8. the clamping part of resilient clamp: will hatch frame and microslide and force together to form and hatch cell.
9. the clamp port of resilient clamp: resilient clamp is clamped in and is hatched on frame and microslide by this clamp port.
10. the Space adjustment part of camera incubata Space adjustment piece: insert and hatch cell, regulate the size in camera incubata space.
The dovetail convex body of 11. camera incubata Space adjustment pieces: insert and hatch in frame in the dovetail groove in rectangular wall, prevent that regulating block is hatching migration in cell.
12. camera incubata upper covers: its effect is set forth in 1,2.
13. hatch cell: form small space and deposit Incubating Solution, accomplish both to use micro-Incubating Solution, guaranteed that again the histotomy on microslide is immersed in Incubating Solution all the time.
14. resilient clamps.
Embodiment
A microslide couveuse for detachable, volume adjustable and vaporization prevention, comprises that stainless steel hatches frame, and stainless steel is hatched frame and is pressed on microslide, jointly by middle coffin, take microslide, as bottom surface, is formed and hatches cell with the silica gel bed course below it; Described silica gel bed course has with stainless steel hatches the intermediate rectangular space that frame is identical, and sticks to stainless steel and hatch on frame, contacts with microslide, prevents the leakage of Incubating Solution; Stainless steel hatches the peripheral dimension of frame and the peripheral dimension of silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched to frame and silica gel bed course is clamped in the resilient clamp on microslide; Described resilient clamp comprises the clamping part of resilient clamp root, resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and the hand position of resilient clamp when forming microslide couveuse and the operation of dismounting microslide couveuse is provided; Clamp port by resilient clamp is clamped in resilient clamp to hatch on frame and microslide, and the clamping part of resilient clamp will be hatched frame and microslide and is clamped together to form and hatch cell; The inwall four sides that stainless steel is hatched frame and silica gel bed course arranges respectively a dovetail groove, dovetail groove is pegged graft and is coordinated with the camera incubata Space adjustment piece with swallow-tail form convex body, regulate the size in camera incubata space, prevent that camera incubata Space adjustment piece is hatching migration in cell; Separately there is camera incubata upper cover cover plate to cover and hatching frame upper surface, prevent from hatching the evaporation of moisture in Incubating Solution in cell; Camera incubata upper cover handle is set on camera incubata upper cover cover plate, by hand-held this handle, opens or cover camera incubata upper cover.
The microslide of selecting according to immunohistochemical requirement processing, to guarantee that histotomy has good sticking effect on microslide.Choose the required tissue of experiment and cut into slices, and be attached on microslide.The histotomy being attached on microslide is carried out to the sealing processing before antibody incubation.Complete after above-mentioned experimental procedure, stainless steel is hatched to frame (3) and silica gel bed course (4) and be pressed in that to be pasted with the microslide (6) of histotomy upper, guarantee that all histotomies are all hatching the bottom of hatching cell (13) of frame with microslide formation.Any one side in frame and upper and lower overlapping four limits of microslide is hatched in selection, hand-held resilient clamp root (7), the clamp port of resilient clamp (9) is aimed at and hatched the structure that frame and microslide are stacked up and down, resilient clamp is pushed and hatches the structure that frame and microslide are stacked up and down, the clamping part (8) that guarantees resilient clamp will be hatched frame and microslide forcing together tightly, and its excess-three limit is repeated above-mentioned action resilient clamp (14) is sandwiched.Guarantee to hatch frame and microslide close contact, form the cell of hatching not leaking.Form hatching after cell of not leaking, the position attaching on microslide according to histotomy, optionally to hatching, in frame, in 4 dovetail grooves in rectangular wall, insert 1 to 4 camera incubata Space adjustment piece, camera incubata Space adjustment piece is comprised of Space adjustment part (10) and the swallow-tail form convex body (11) of camera incubata Space adjustment piece, with flexible, hatch rectangle in frame arbitrarily or the size in polygon space on one side, insert camera incubata Space adjustment piece and do not hide the histotomy that is attached at camera incubata bottom as standard after inserting.Regulate after the size in camera incubata space, to hatching the Incubating Solution adding in cell containing special first antibody, be sure oing to stick on the histotomy of hatching cell bottom can be immersed in containing after the Incubating Solution of special first antibody, hand-held camera incubata upper cover handle, camera incubata upper cover cover plate is covered at stainless steel and hatches frame upper surface, prevent because of moisture in Incubating Solution evaporation change the concentration of volume and the first antibody of Incubating Solution.Then carry out on request hatching of histotomy.After first antibody has been hatched, hand-held camera incubata upper cover handle is opened camera incubata upper cover cover plate, sucking-off is containing the Incubating Solution of special first antibody, extract out to insert and hatch the camera incubata Space adjustment piece in rectangular wall dovetail groove in frame, take off and hatch frame and the microslide resilient clamp on phase stack structure four limits up and down, from microslide, unload and hatch frame.Routinely the histotomy sticking on microslide is carried out to carrying out washing treatment.Need to carry out second antibody while hatching, repeat above-mentioned action, again form camera incubata space size suitable and prevent the cell of hatching of moisture evaporation in Incubating Solution.Add the Incubating Solution containing special second antibody, histotomy is hatched.After having hatched, open camera incubata upper cover, remove containing special second antibody Incubating Solution.Repeat above-mentioned action, under dismounting, hatch frame.To sticking on histotomy on microslide, wash, the aftertreatment such as dehydration, transparent, mounting.After all finishing dealing with, examine under a microscope the immunohistochemical staining result of histotomy.
Claims (2)
1. the microslide couveuse of detachable a, volume adjustable and vaporization prevention, it is characterized in that: comprise that stainless steel hatches frame, stainless steel is hatched frame and is pressed on microslide, jointly by middle coffin, take microslide, as bottom surface, is formed and hatches cell with the silica gel bed course below it; Described silica gel bed course has with stainless steel hatches the intermediate rectangular space that frame is identical, and sticks to stainless steel and hatch on frame, contacts with microslide, prevents the leakage of Incubating Solution; Stainless steel hatches the peripheral dimension of frame and the peripheral dimension of silica gel bed course is all identical with microslide peripheral dimension; Separately be provided with and stainless steel hatched to frame and silica gel bed course is clamped in the resilient clamp on microslide; Described resilient clamp comprises the clamping part of resilient clamp root, resilient clamp and the clamp port of resilient clamp; Resilient clamp root connects resilient clamp clamping part, and the hand position of resilient clamp when forming microslide couveuse and the operation of dismounting microslide couveuse is provided; Clamp port by resilient clamp is clamped in resilient clamp to hatch on frame and microslide, and the clamping part of resilient clamp will be hatched frame and microslide and is clamped together to form and hatch cell; The inwall four sides that stainless steel is hatched frame and silica gel bed course arranges respectively a dovetail groove, dovetail groove is pegged graft and is coordinated with the camera incubata Space adjustment piece with swallow-tail form convex body, regulate the size in camera incubata space, prevent that camera incubata Space adjustment piece is hatching migration in cell; Separately there is camera incubata upper cover cover plate to cover and hatching frame upper surface, prevent from hatching the evaporation of moisture in Incubating Solution in cell; Camera incubata upper cover handle is set on camera incubata upper cover cover plate, by hand-held this handle, opens or cover camera incubata upper cover.
2. the microslide couveuse of detachable, volume adjustable according to claim 1 and vaporization prevention, it is characterized in that: using method is: stainless steel is hatched to frame and silicagel pad is laminated on the microslide that is pasted with histotomy, guarantee the bottom of hatching cell that all histotomies are all being hatched frame and microslide and formed.The any one side in frame and upper and lower overlapping four limits of microslide is hatched in selection, hand-held resilient clamp root, the clamp port of resilient clamp is aimed at and hatched the structure that frame and microslide are stacked up and down, resilient clamp is pushed and hatches the structure that frame and microslide are stacked up and down, the clamping part that guarantees resilient clamp will be hatched frame and microslide forcing together tightly, its excess-three limit is repeated above-mentioned action resilient clamp is sandwiched, guarantee to hatch frame and microslide close contact, form the cell of hatching not leaking, form hatching after cell of not leaking, the position attaching on microslide according to histotomy, optionally to hatching, in frame, in 4 dovetail grooves in rectangular wall, insert 1 to 4 camera incubata Space adjustment piece, with flexible, hatch rectangle in frame arbitrarily or the size in polygon space on one side, regulate after the size in camera incubata space, to hatching the Incubating Solution that adds first antibody in cell, assurance sticks on the histotomy of hatching cell bottom and can be immersed in after the Incubating Solution of first antibody, hand-held camera incubata upper cover handle, camera incubata upper cover cover plate is covered at stainless steel and hatches frame upper surface, prevent because of moisture in Incubating Solution evaporation change the concentration of volume and the first antibody of Incubating Solution, then carry out on request hatching of histotomy, after first antibody has been hatched, hand-held camera incubata upper cover handle is opened camera incubata upper cover cover plate, the Incubating Solution of sucking-off first antibody, extract out to insert and hatch the camera incubata Space adjustment piece in rectangular wall dovetail groove in frame, take off and hatch frame and the microslide resilient clamp on phase stack structure four limits up and down, from microslide, unload and hatch frame, the histotomy sticking on microslide is carried out to carrying out washing treatment.Need to carry out second antibody while hatching, repeat above-mentioned action.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510031613.6A CN104614512B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of detachable, the volume adjustable that experimental result is good and vaporization prevention |
| CN201510031557.6A CN104597234B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of rational in infrastructure, detachable, volume adjustable and vaporization prevention |
| CN201410012875.3A CN103743896B (en) | 2014-01-13 | 2014-01-13 | Detachable, volume adjustable and evaporation-prevention glass slide incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410012875.3A CN103743896B (en) | 2014-01-13 | 2014-01-13 | Detachable, volume adjustable and evaporation-prevention glass slide incubator |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510031613.6A Division CN104614512B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of detachable, the volume adjustable that experimental result is good and vaporization prevention |
| CN201510031557.6A Division CN104597234B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of rational in infrastructure, detachable, volume adjustable and vaporization prevention |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103743896A true CN103743896A (en) | 2014-04-23 |
| CN103743896B CN103743896B (en) | 2015-04-15 |
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| CN201410012875.3A Expired - Fee Related CN103743896B (en) | 2014-01-13 | 2014-01-13 | Detachable, volume adjustable and evaporation-prevention glass slide incubator |
| CN201510031557.6A Expired - Fee Related CN104597234B (en) | 2014-01-13 | 2014-01-13 | The microslide couveuse of rational in infrastructure, detachable, volume adjustable and vaporization prevention |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095759A (en) * | 1993-02-16 | 1994-11-30 | 帕金-埃尔默有限公司 | The in-situ polymerization PCR amplification system |
| WO1999058954A1 (en) * | 1998-05-13 | 1999-11-18 | Bayer Corporation | Optical spectroscopy sample cell |
| WO1999063342A1 (en) * | 1998-06-02 | 1999-12-09 | Ventana Medical Systems, Inc. | Method for staining biological specimens by combining unstable reagents on a microscope slide |
| CN1335501A (en) * | 2001-04-27 | 2002-02-13 | 上海晶泰生物技术有限公司 | Multiple-sample microarray biochip |
| CN1882382A (en) * | 2003-11-24 | 2006-12-20 | 生物概念股份有限公司 | Microarray hybridization device |
| WO2008002951A2 (en) * | 2006-06-29 | 2008-01-03 | Ge Healthcare Bio-Sciences Ab | Chamber apparatus |
| CN202433235U (en) * | 2012-01-30 | 2012-09-12 | 上海海洋大学 | Clamp for fixing glass slides |
| CN103344760A (en) * | 2013-07-23 | 2013-10-09 | 成都安铂奥金生物科技有限公司 | High-throughput immunohistochemical detection method and multi-sample immunohistochemical detection board |
| CN203350255U (en) * | 2013-07-02 | 2013-12-18 | 武汉三鹰生物技术有限公司 | Adjustable horizontal incubation box |
-
2014
- 2014-01-13 CN CN201410012875.3A patent/CN103743896B/en not_active Expired - Fee Related
- 2014-01-13 CN CN201510031557.6A patent/CN104597234B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095759A (en) * | 1993-02-16 | 1994-11-30 | 帕金-埃尔默有限公司 | The in-situ polymerization PCR amplification system |
| WO1999058954A1 (en) * | 1998-05-13 | 1999-11-18 | Bayer Corporation | Optical spectroscopy sample cell |
| WO1999063342A1 (en) * | 1998-06-02 | 1999-12-09 | Ventana Medical Systems, Inc. | Method for staining biological specimens by combining unstable reagents on a microscope slide |
| CN1335501A (en) * | 2001-04-27 | 2002-02-13 | 上海晶泰生物技术有限公司 | Multiple-sample microarray biochip |
| CN1882382A (en) * | 2003-11-24 | 2006-12-20 | 生物概念股份有限公司 | Microarray hybridization device |
| WO2008002951A2 (en) * | 2006-06-29 | 2008-01-03 | Ge Healthcare Bio-Sciences Ab | Chamber apparatus |
| CN202433235U (en) * | 2012-01-30 | 2012-09-12 | 上海海洋大学 | Clamp for fixing glass slides |
| CN203350255U (en) * | 2013-07-02 | 2013-12-18 | 武汉三鹰生物技术有限公司 | Adjustable horizontal incubation box |
| CN103344760A (en) * | 2013-07-23 | 2013-10-09 | 成都安铂奥金生物科技有限公司 | High-throughput immunohistochemical detection method and multi-sample immunohistochemical detection board |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104597234A (en) | 2015-05-06 |
| CN104597234B (en) | 2016-03-16 |
| CN103743896B (en) | 2015-04-15 |
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