CN103735709A - Compound Ilex purpurea hassk ointment as well as preparation method and detection method thereof - Google Patents
Compound Ilex purpurea hassk ointment as well as preparation method and detection method thereof Download PDFInfo
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Abstract
The invention provides a compound Ilex purpurea hassk ointment as well as a preparation method and a detection method thereof. The compound Ilex purpurea hassk ointment is made from 100 g of Ilex purpurea hassk, 80 g of rhubarb, 60 g of lithospermum, 40 g of honeysuckle, 20 g of rhizoma corydalis, 4 g of borneol and 1000 g of yellow Vaseline. Aiming at the defects of the prior art, the preparation process of the compound Ilex purpurea hassk ointment is optimized to ensure better curative effects of clearing away heat and toxic materials, relieving swelling and pain, astringing and promoting regeneration of tissue, a content measurement method of L-shikonin contained in the monarch drug lithospermum is established, and is high in precision, good in accuracy and capable of effectively controlling the quality of the medicine, thereby ensuring the clinical curative effect of the medicine.
Description
Technical field
The present invention relates to a kind of Compound Sijiqing ointment machin its preparation method and detection method, belong to technical field of Chinese medicines.
Background technology
Purple handle Ilicis Purpureae ointment is made take purple handle Ilicis Purpureae, Radix Arnebiae (Radix Lithospermi), Radix Et Rhizoma Rhei, Flos Lonicerae, Borneolum Syntheticum and Rhizoma Corydalis as crude drug, there is the effect of heat-clearing and toxic substances removing, reducing swelling and alleviating pain, convergence granulation promoting, aspect the healing of the healing to burn, scald, wound and infected wound and ulcer surface, obtaining good effect.
Traditional preparation technology of purple handle Ilicis Purpureae ointment is: first take 1000g Yellow Vaselin heat fused, add Radix Arnebiae (Radix Lithospermi) 60g with 1 hour at a simmer, the Radix Et Rhizoma Rhei 80g, the Rhizoma Corydalis 20g that add recipe quantity add the Flos Lonicerae 40g of recipe quantity, purple handle Ilicis Purpureae 100g with 1 hour at a simmer with at a simmer after sallow, by 4 layers of filtered through gauze, after slightly cold, add Borneolum Syntheticum 4g, stir it is dissolved completely, let cool, subpackage, obtains.Preparation prepared by this technique, the alkannin content of one of its monarch drug is on the low side, and that shikonin has is anticancer, antiinflammatory, the effect such as antibacterial, its content height has directly affected the clinical efficacy of purple handle Ilicis Purpureae ointment, is its important effective ingredient.
Thus, the assay of shikonin is also the important indicator in the quality testing of purple handle Ilicis Purpureae ointment.At present, Chinese Pharmacopoeia only records the content assaying method of hydroxyl naphthoquinone total pigment in Radix Arnebiae (Radix Lithospermi) medical material, if adopt the method to measure the content of shikonin in purple handle Ilicis Purpureae ointment, its result error is larger.Current purple handle Ilicis Purpureae ointment does not also have strict, reliable, complete quality inspection standard.If there is no strict quality standard, the product obtaining can not be guaranteed its quality, and result will affect the clinical efficacy of this medicine; Think and improve the therapeutical effect of purple handle Ilicis Purpureae ointment, guarantee the safe, effectively and product quality stable of medication, formulates a strict reliable quality standard very necessary.
In addition, through medical material expert's discriminating, assert purple handle Ilicis Purpureae in purple handle Ilicis Purpureae ointment actual be exactly Ilex purpurea Hassk.[I.chinensis Sims in pharmacopeia, purple handle Ilicis Purpureae be only local.Therefore, the application renames purple handle Ilicis Purpureae ointment as Compound Sijiqing ointment.
Summary of the invention
The object of the invention is to, a kind of Compound Sijiqing ointment machin its preparation method and detection method are provided.The present invention is directed to the deficiencies in the prior art, preparation technology to Compound Sijiqing ointment is optimized, make its curative effect to heat-clearing and toxic substances removing, reducing swelling and alleviating pain, convergence granulation promoting more remarkable, and set up the content assaying method of contained Shikonin in monarch drug Radix Arnebiae (Radix Lithospermi), its precision is high, accuracy good, can effectively control the quality of this medicine, thereby guarantee its clinical efficacy.
Technical scheme of the present invention is as follows:
A kind of Compound Sijiqing ointment, is made by Ilex purpurea Hassk.[I.chinensis Sims 100g, Radix Et Rhizoma Rhei 80g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Lonicerae 40g, Rhizoma Corydalis 20g, Borneolum Syntheticum 4g and Yellow Vaselin 1000g.
The preparation method of aforesaid Compound Sijiqing ointment is: first take 200g Yellow Vaselin heat fused, add Radix Arnebiae (Radix Lithospermi) 60g with 1 hour at a simmer, by 4 layers of filtered through gauze, filtrate and residue all stay standby; Again by 800g Yellow Vaselin heat fused, add Radix Et Rhizoma Rhei 80g, Rhizoma Corydalis 20g with at a simmer to after sallow, add again Flos Lonicerae 40g, Ilex purpurea Hassk.[I.chinensis Sims 100g and Radix Arnebiae (Radix Lithospermi) residue with 1 hour at a simmer, by 4 layers of filtered through gauze, merge twice filtrate, add Borneolum Syntheticum 4g after slightly cold, stirring is dissolved it completely, let cool, subpackage, obtains.
The detection method of aforesaid Compound Sijiqing ointment comprises assay project; This assay is take Shikonin reference substance as contrast, adopts ultraviolet visible spectrophotometry at 516nm wavelength place, to measure the content of the contained Shikonin of Radix Arnebiae (Radix Lithospermi) in preparation.
In aforementioned detection method, concrete content assaying method is:
(1) preparation of reference substance solution: precision takes (100,000/balance) Shikonin reference substance 5.04mg, puts in 50mL measuring bottle, adds dissolve with ethanol and is diluted to scale, shakes up, in contrast product solution;
(2) preparation of need testing solution: take this preparation 5g, put in small beaker, add kieselguhr 5g, after stirring evenly, move in tool plug conical flask, add 100mL ethanol, ultrasonic 40min in 50 ℃ of water-baths, lets cool, standing, filter, precision measures in subsequent filtrate 5mL to 25mL measuring bottle, adds ethanol to scale, shake up, as need testing solution;
(3) drafting of standard curve: accurate reference substance solution 1.4,2.8,4.2,5.6,7.0, the 8.4mL of drawing, put respectively in 25mL measuring bottle, add ethanol to scale, shake up, take the ethanol of equivalent as blank, according to the ultraviolet visible spectrophotometry of second appendix V A of Chinese Pharmacopoeia, in 516nm wavelength place, measure absorbance, take concentration as abscissa, absorbance is vertical coordinate drawing standard curve, through linear regression, obtains regression equation;
(4) algoscopy: precision measures need testing solution 4.2mL, puts in 25mL measuring bottle, adds ethanol to scale, according to the method under Specification Curve of Increasing item, measures absorbance, and substitution regression equation, by Shikonin C
16h
16o
5absorptance
be 242 to calculate the content of Shikonin in this preparation.
In order to ensure medicament composing prescription of the present invention and preparation technology's science, reasonable, feasible thereof, applicant has carried out series of experimental research and Effect tests.
(1) clinical observation case (clinical practice)
1, effect and usage
Compound Sijiqing ointment of the present invention has the effect of heat-clearing and toxic substances removing, reducing swelling and alleviating pain, convergence granulation promoting.Treatment burn is that this ointment is made to medicine yarn, with there being aseptic " the medicine yarn " of Compound Sijiqing ointment to spread on wound surface, then covers antiseptic gauze immobilization with adhesive tape, and change dressings every day.For children's's lip crocodile, split the postoperative infection of changing dressings, with sterilization shears, be cut into small pieces, on photograph, method is changed dressings.
2, observation of curative effect (summary case)
(1) clinical data
Select 216 routine harelip patient with operation, carry out post-operative wound medication observation.Symptom mostly be lip edge of wound have in various degree red and swollen or have ooze out, tenderness etc.
Be divided at random treatment group and matched group.Treatment group 133 examples, male 87 examples, female's 46 examples, age 1-32 year; Matched group 128 examples, male 86 examples, female's 42 examples, age 1-28 year.Two groups of physical data contrast, and the situations such as its sex, age, severity extent, cardinal symptom are learned and processed by statistics, P ﹥ 0.05, and no significant difference, has comparability.
(2) Therapeutic Method
Treatment group: Compound Sijiqing ointment of the present invention is applied on the postoperative lip edge of wound of harelip, with antiseptic gauze covering, once a day, changes altogether 3 times; Matched group: use same method to change chlorotetracycline eye ointment into and be applied on lip edge of wound, once a day, totally 3 times.
(3) efficacy assessment standard
Effective: symptom disappears substantially, swelling, ooze out major part and disappear; Effective: symptom is improved, swelling is disappeared; Invalid: symptom is not improved.
(4) therapeutic outcome
Table 1 observation group and the comparison for the treatment of of control group result
As seen from the above table, the treated effect for the treatment of group is significantly better than matched group (P ﹥ 0.01).
(2) preparation technology investigates
1, the drug effect of two kinds of techniques contrast
Adopt traditional handicraft (Radix Arnebiae (Radix Lithospermi) in prescription is only extracted once) prepare Compound Sijiqing ointment, clinical observation light Ⅱ° burn and scald patient 10 examples; Adopt the present invention's light Ⅱ° burn and scald patient 18 examples of having improved Compound Sijiqing ointment clinical observation prepared by preparation technology's (Radix Arnebiae (Radix Lithospermi) extract twice) in prescription.Efficacy assessment standard: effective: symptom disappears substantially, swelling, oozes out major part and disappears; Effective: symptom is improved, swelling is disappeared; Invalid: symptom is not improved.
Clinical efficacy to two groups is observed, and therapeutic outcome is in Table 1.What show Compound Sijiqing ointment that preparation technology of the present invention prepares is evident in efficacyly better than Compound Sijiqing ointment prepared by traditional handicraft.
The observation of curative effect result of Compound Sijiqing ointment prepared by two kinds of techniques of table 2
| Group | Case load/example | Effective/example | Effectively/example | Invalid/example |
| Traditional handicraft | 10 | 2 | 8 | 0 |
| The present invention improves technique | 18 | 16 | 2 | 0 |
2, the alkannin content of two kinds of techniques contrast
Adopt traditional handicraft, the present invention to improve technique (extracted twice method) and prepare respectively Compound Sijiqing ointment, adopt ultraviolet visible spectrophotometry at 516nm wavelength place, to measure absorbance, calculate the content of Shikonin, the results are shown in Table 3.
Table 3
As can be seen here, the Compound Sijiqing ointment of preparing according to traditional handicraft, one of monarch drug alkannin content is on the low side, and after changing extracted twice method into, alkannin content is significantly improved, and the curative effect of said preparation is corresponding to be also improved.
In addition, in order to ensure detection method science of the present invention, reasonable, feasible, applicant has carried out series of experimental research and investigation.
(1) assay of Shikonin:
1, instrument and reagent
Instrument: UV2501 type-ultraviolet spectrophotometer (Japanese Shimadzu business administration company limited) electronic balance (Switzerland Mei Te company, model: XS205); Analytical balance (manufacturer Shanghai, model: AB204-S); Ultrasonic washing unit (Tianjin is difficult to understand safe, model: HS10260D).
Reagent: 95% ethanol (analytical pure, lot number 20110401, Chongqing Chuan Dong chemical industry (group) company limited chemical reagent factory); Kieselguhr (chemical pure, lot number 090605, Chengdu Ke Long chemical reagent factory).
Reference substance: Shikonin reference substance (lot number 0769-9903, Nat'l Pharmaceutical & Biological Products Control Institute).
Sample: Compound Sijiqing ointment provides (lot number 20121003) by the Drug Manufacturing Room of Hospital Attached to Guiyang Medical College.
2, the preparation of reference substance solution
Precision takes (100,000/balance) Shikonin reference substance 5.04mg, puts in 50mL measuring bottle, adds dissolve with ethanol and be diluted to scale to shake up product solution in contrast.
3, the preparation of need testing solution
Take this preparation 5g, put in small beaker, add kieselguhr 5g, after stirring evenly, move in tool plug conical flask, add 100mL ethanol, in 50 ℃ of water-baths ultrasonic 40 minutes, let cool, standing, filter, precision is got in subsequent filtrate 5mL to 25mL measuring bottle, adds ethanol to scale, shakes up, and obtains.
4, the mensuration of Shikonin reference substance maximum absorption wavelength
Blank assay: get Shikonin reference substance ethanol, take ethanol as blank, in the interscan of 600～400nm wave-length coverage, Shikonin reference substance has absorption maximum at 516nm place, as shown in Figure 1.
5, sample uv absorption figure measures
Get Compound Sijiqing ointment sample 10.0g, by " preparation of a need testing solution " lower preparation, on UV-2501 ultraviolet spectrophotometer, in the interscan of 600～400nm wave-length coverage, at 516nm place, have absorption maximum.As shown in Figure 2.
6, lack Radix Arnebiae (Radix Lithospermi) negative sample contrast liquid interference test
Get and lack Radix Arnebiae (Radix Lithospermi) negative sample 10.0g, by operating with method under " preparation of need testing solution " item, on UV-2501 ultraviolet spectrophotometer in the interscan of 800～400nm wave-length coverage, lack Radix Arnebiae (Radix Lithospermi) negative controls at 516nm place substantially without absorption, and absorbance is extremely low, can ignore, as shown in Figure 3.
7, the investigation of linear relationship
Accurate reference substance solution 1.4,2.8,4.2,5.6,7.0, the 8.4mL of drawing, puts respectively in 25mL measuring bottle, adds ethanol to scale, shakes up, and take the ethanol of equivalent as blank, measures absorbance in λ=516nm wavelength place, and measurement result is in Table 4.Take concentration as abscissa, absorbance is vertical coordinate drawing standard curve, through linear regression, obtain regression equation: y=23.157x+0.0017, R=0.9999, Shikonin is good linear relation at 5.64-33.87 μ g/mL scope internal absorbance A and concentration C, as shown in Figure 4.
Table 4
8, precision test
Get same reference substance solution and be measured in the same method 6 times, the results are shown in Table 5, RSD(%)=0.13%(n=6), show that instrument precision is good.
Table 5
9, replica test
Take with 6 parts, batch (lot number is 20121112) sample, each 5g, respectively by standby with legal system under " preparation of need testing solution " item, in 516nm place, measure various kinds product absorbance, calculate its Shikonin content, the results are shown in Table 6, measurement result RSD(%)=2.2%(n=6), illustrate that repeatability is good.
Table 6
10, stability test
Get same need testing solution, respectively 0.5,1,1.5,2,2.5,3h, on UV-2501 ultraviolet spectrophotometer, 516nm wavelength place measures its absorbance, measured value RSD(%)=1.4%(n=6), the results are shown in Table 7, show that need testing solution is basicly stable in 3 hours.
Table 7
11, average recovery test
Take the about 2.5g of same batch sample, Shikonin reference substance 4.03mg puts in small beaker, adds kieselguhr 2.5g, after stirring evenly, move in tool plug conical flask, add 100mL ethanol, in 50 ℃ of water-baths ultrasonic 40 minutes, let cool, standing, filter, get in subsequent filtrate 5mL to 25mL measuring bottle, measure absorbance, with method operation 6 times, calculate its response rate, average recovery rate is 98.8092%, RSD (%)=0.8849%(n=6), concrete outcome is in Table 8.
Table 8
12, sample size is measured
Precision takes each batch of sample 5g, by " preparation of a need testing solution " lower preparation, measures absorbance at 516nm wavelength place, and the content of Shikonin in calculation sample, the results are shown in Table 9.
Table 9
(3) sample size is measured contrast
In the Radix Arnebiae (Radix Lithospermi) medical material that employing Chinese Pharmacopoeia is recorded, the content assaying method of Shikonin is measured the Shikonin content of Compound Sijiqing ointment sample of the present invention, be specially: take sample appropriate, put in 100mL beaker, add appropriate kieselguhr, after stirring with glass rod, add appropriate ethanol stirring and dissolving, be transferred in 100mL measuring bottle, add ethanol to scale, 4 little in jolting constantly, filter; Precision measures subsequent filtrate 5mL, puts in 25mL measuring bottle, adds ethanol to scale, shakes up, and according to ultraviolet visible spectrophotometry (appendix VA), measures absorbance, by Shikonin (C at 516nm wavelength place
16h
16o
5) absorptance
be 242 calculating, obtain.The results are shown in Table 10.
The result (n=3) that table 10 official method is measured sample size
Adopt Shikonin content assaying method of the present invention to carry out assay to the Compound Sijiqing ointment sample of same batch, be specially: take sample appropriate, put in 100mL beaker, add appropriate kieselguhr, after stirring with glass rod, add appropriate ethanol stirring and dissolving, be transferred in 100mL measuring bottle, add ethanol to scale, supersound process 40 minutes in 50 ℃ of water-baths, standing, filter; Precision measures subsequent filtrate 5mL, puts in 25mL measuring bottle, adds ethanol to scale, shakes up, and according to ultraviolet visible spectrophotometry, measures absorbance at 516nm wavelength place, the results are shown in Table 11.
The result (n=3) that table 11 the inventive method is measured sample size
From above-mentioned test, in the Compound Sijiqing ointment that in the Radix Arnebiae (Radix Lithospermi) medical material that employing pharmacopeia is recorded, the content assaying method of Shikonin records, content and the actual content deviation of Shikonin are larger, are not suitable for the content assaying method as Shikonin in Compound Sijiqing ointment.
Compared with prior art, the present invention improves the preparation technology of Compound Sijiqing ointment, makes the main active substances of heat-clearing and toxic substances removing, reducing swelling and alleviating pain, convergence granulation promoting in medical material extract more abundant, and its clinical efficacy is better; And, the present invention is directed to Compound Sijiqing ointment after improvement and set up the content assaying method of contained Shikonin in monarch drug Radix Arnebiae (Radix Lithospermi), the precision of described method is high, favorable reproducibility, the response rate is high, measurement result is accurate, has reached the object of effective control drug quality, thereby has guaranteed stable and clinical application safe, effective of product quality.
Accompanying drawing explanation
Fig. 1 is the uv absorption figure of Shikonin reference substance;
Fig. 2 is the uv absorption figure of Compound Sijiqing ointment sample;
Fig. 3 is the uv absorption figure that lacks Radix Arnebiae (Radix Lithospermi) negative sample;
Fig. 4 is the canonical plotting of Shikonin assay.
The specific embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1: a kind of Compound Sijiqing ointment, by Ilex purpurea Hassk.[I.chinensis Sims 100g, Radix Et Rhizoma Rhei 80g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Lonicerae 40g, Rhizoma Corydalis 20g, Borneolum Syntheticum 4g and Yellow Vaselin 1000g, made.
The preparation method of this Compound Sijiqing ointment: first take 200g Yellow Vaselin heat fused, add Radix Arnebiae (Radix Lithospermi) 60g with 1 hour at a simmer, by 4 layers of filtered through gauze, filtrate and residue all stay standby; Again by 800g Yellow Vaselin heat fused, add Radix Et Rhizoma Rhei 80g, Rhizoma Corydalis 20g with at a simmer to after sallow, add again Flos Lonicerae 40g, Ilex purpurea Hassk.[I.chinensis Sims 100g and Radix Arnebiae (Radix Lithospermi) residue with 1 hour at a simmer, by 4 layers of filtered through gauze, merge twice filtrate, add Borneolum Syntheticum 4g after slightly cold, stirring is dissolved it completely, let cool, subpackage, obtains Compound Sijiqing ointment.
The content assaying method of the contained Shikonin of Radix Arnebiae (Radix Lithospermi) in the prepared Compound Sijiqing ointment of embodiment 2: embodiment 1, specifically comprises the following steps:
1. the preparation of reference substance solution: precision takes (100,000/balance) Shikonin reference substance 5.04mg, puts in 50mL measuring bottle, adds dissolve with ethanol and is diluted to scale, shakes up, in contrast product solution;
2. the preparation of need testing solution: take this preparation 5g, put in small beaker, add kieselguhr 5g, after stirring evenly, move in tool plug conical flask, add 100mL ethanol, ultrasonic 40min in 50 ℃ of water-baths, lets cool, standing, filter, precision measures in subsequent filtrate 5mL to 25mL measuring bottle, adds ethanol to scale, shake up, as need testing solution;
3. the drafting of standard curve: accurate reference substance solution 1.4,2.8,4.2,5.6,7.0, the 8.4mL of drawing, put respectively in 25mL measuring bottle, add ethanol to scale, shake up, take the ethanol of equivalent as blank, ultraviolet visible spectrophotometry according to second appendix V A of Chinese Pharmacopoeia is measured absorbance in 516nm wavelength place, take concentration C as abscissa, absorbance A is vertical coordinate drawing standard curve, through linear regression, obtain regression equation: y=23.157x+0.0017, R=0.9999;
4. algoscopy: precision measures need testing solution 4.2mL, puts in 25mL measuring bottle, adds ethanol to scale, according to the method under Specification Curve of Increasing item, measures absorbance, and substitution regression equation, by Shikonin C
16h
16o
5absorptance
be 242 to calculate the content of Shikonin in this preparation.
Claims (4)
1. a Compound Sijiqing ointment, is characterized in that: it is made by Ilex purpurea Hassk.[I.chinensis Sims 100g, Radix Et Rhizoma Rhei 80g, Radix Arnebiae (Radix Lithospermi) 60g, Flos Lonicerae 40g, Rhizoma Corydalis 20g, Borneolum Syntheticum 4g and Yellow Vaselin 1000g.
2. the preparation method of Compound Sijiqing ointment as claimed in claim 1, is characterized in that: first take 200g Yellow Vaselin heat fused, add Radix Arnebiae (Radix Lithospermi) 60g with 1 hour at a simmer, by 4 layers of filtered through gauze, filtrate and residue all stay standby; Again by 800g Yellow Vaselin heat fused, add Radix Et Rhizoma Rhei 80g, Rhizoma Corydalis 20g with at a simmer to after sallow, add again Flos Lonicerae 40g, Ilex purpurea Hassk.[I.chinensis Sims 100g and Radix Arnebiae (Radix Lithospermi) residue with 1 hour at a simmer, by 4 layers of filtered through gauze, merge twice filtrate, add Borneolum Syntheticum 4g after slightly cold, stirring is dissolved it completely, let cool, subpackage, obtains.
3. the detection method of Compound Sijiqing ointment as claimed in claim 1, is characterized in that: described detection method comprises assay project; This content assaying method is take Shikonin reference substance as contrast, adopts ultraviolet visible spectrophotometry at 516nm wavelength place, to measure the content of the contained Shikonin of Radix Arnebiae (Radix Lithospermi) in preparation.
4. the detection method of Compound Sijiqing ointment according to claim 3, is characterized in that: concrete content assaying method is:
(1) preparation of reference substance solution: precision takes Shikonin reference substance 5.04mg, puts in 50mL measuring bottle, adds dissolve with ethanol and is diluted to scale, shakes up, in contrast product solution;
(2) preparation of need testing solution: take this preparation 5g, put in small beaker, add kieselguhr 5g, after stirring evenly, move in tool plug conical flask, add 100mL ethanol, ultrasonic 40min in 50 ℃ of water-baths, lets cool, standing, filter, precision measures in subsequent filtrate 5mL to 25mL measuring bottle, adds ethanol to scale, shake up, as need testing solution;
(3) drafting of standard curve: accurate reference substance solution 1.4,2.8,4.2,5.6,7.0, the 8.4mL of drawing, put respectively in 25mL measuring bottle, add ethanol to scale, shake up, take the ethanol of equivalent as blank, according to the ultraviolet visible spectrophotometry of second appendix V A of Chinese Pharmacopoeia, in 516nm wavelength place, measure absorbance, take concentration as abscissa, absorbance is vertical coordinate drawing standard curve, through linear regression, obtains regression equation;
(4) algoscopy: precision measures need testing solution 4.2mL, puts in 25mL measuring bottle, adds ethanol to scale, according to the method under Specification Curve of Increasing item, measures absorbance, and substitution regression equation calculates the content of Shikonin in this preparation.
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| CN102462764A (en) * | 2010-11-15 | 2012-05-23 | 卢晓峰 | No.2 burn oil |
| RU2473329C2 (en) * | 2011-03-29 | 2013-01-27 | Муниципальное учреждение здравоохранения "Клиническая поликлиника № 5" г. Кемерово | Ointment for treating bitten wounds |
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2013
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| CN102397389A (en) * | 2010-09-08 | 2012-04-04 | 朱云利 | Preparation method of lithospermum ointment |
| CN102462764A (en) * | 2010-11-15 | 2012-05-23 | 卢晓峰 | No.2 burn oil |
| RU2473329C2 (en) * | 2011-03-29 | 2013-01-27 | Муниципальное учреждение здравоохранения "Клиническая поликлиника № 5" г. Кемерово | Ointment for treating bitten wounds |
| KR20130093820A (en) * | 2012-01-18 | 2013-08-23 | (재)예수병원유지재단 | Method for preparing japonica extract and cream and band comprising the same |
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