CN103735539A - Use of coenzyme Q10 - Google Patents
Use of coenzyme Q10 Download PDFInfo
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- CN103735539A CN103735539A CN201410025463.3A CN201410025463A CN103735539A CN 103735539 A CN103735539 A CN 103735539A CN 201410025463 A CN201410025463 A CN 201410025463A CN 103735539 A CN103735539 A CN 103735539A
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Abstract
The invention discloses a coenzyme Q10, belonging to the field of a feed additive, and use of a coenzyme Q10 premix in preparation of a drug for preventing and treating podagra. The coenzyme Q10 premix comprises the following components by weight percent: 2-5% of coenzyme Q10, and 95-98% of a carrier. The coenzyme Q10 and the premix thereof disclosed by the invention have significant prevention and treatment effects on avian gout, the renal pathological damage caused by the avian gout of a laying hen can be significantly relieved, the poultry mortality rate is reduced, secondly, the coenzyme Q10 disclosed by the invention does not contain an organic ingredient, and is free of toxic and side effects on birds and beasts and free of toxicity on environment.
Description
The application is that the denomination of invention of December in 2011 proposition on the 12nd is the divisional application of a < < coenzyme Q10 pre-mixing agent and its preparation method and application > > (application number is: 201110410869.x) patent application.
Technical field
The invention belongs to feed additive field, be specifically related to a kind of purposes of coenzyme Q10.
Background technology
Fowl gout is mainly in poultry.Fowl gout is in enormous quantities generation conventionally, and foreign statistic shows that the sickness rate of gout is about 16～50%, and mortality rate is higher.Fowl gout is mainly to cause kidney injury.At present, the morbidity of fowl gout has the trend increasing, reason is that the chicken group of intensive farm is many factors of bringing out gout that exist in Feed Manufacturing, feeding and management level, particularly because feed resource is unstable, causes in feedstuff that calcic and albumen are too high easily causes young laying hen generation gout.Fowl gout is that China's aviculture cultivates one of serious problems that face.For fowl gout, there is no effectively preventing method at present, the medicine for the treatment of fowl gout mainly contains purine alcohol, probenecid, hexamethylenamine, dexamethasone, also have that renal swelling disappears, renal swelling antidote, and some Chinese medicine etc., but effect is all undesirable or have a side effect.
Coenzyme Q10 (CoQ10) is the fat-soluble quinones extensively existing in organism, the number difference of its side chain iso-amylene unit of the ubiquinone of separate sources, and the ubiquinone in the mankind and mammalian body is 10 iso-amylene units, therefore claim coenzyme Q10.Coenzyme Q10 is that cellular metabolism is breathed activator and immunostimulant, has antioxidation, heart tonifying, anti-stress and free radical scavenging function.Coenzyme Q10 is for the auxiliary treatment in diseases such as cardiovascular disease, cancer, hepatitis, parkinson disease, and in aspect application such as sports medical science and antifatigue effects, in cosmetics, oral hygiene goods, health promoting product and food additive, application is also more extensive.
The existing dosage form of coenzyme Q10 comprises: coenzyme Q10 sheet, coenzyme Q 10 soft capsule, coenzyme Q 10 injection, coenzyme Q10 capsule etc.
The patent application of existing coenzyme Q10 dosage form mainly contains: coenzyme q 10 dry (publication number CN1633245A), containing dry powder composite (publication number CN1969833A), compound coenzyme Q 10 medicine preparation (publication number CN101019854A), coenzyme Q 10 injection (publication number CN101278907A), topical co-enzyme Q 10 formulations (publication number CN102018693A), the coenzyme Q 10 mini-pill (publication number CN101703477A) of coenzyme Q10.The subject matter that above-mentioned co-enzyme Q 10 formulations exists is: the dissolubility of coenzyme Q10 in water is extremely low, and water dispersible dry powder will reach the medical function of expection need to take in the amount more much bigger than reality.Injection is large to Animal stress, and workload is large, is not suitable for the administration of veterinary clinic colony.Solution is all organic solvent hydrotropy at present, and toxicity is large, residual height, and the rising of carrier cost, and when this dosage form is for livestock and fowl drinking water simultaneously, medicine is easily separated out.The preparation cost such as micropill and microemulsion is higher, veterinary clinic is promoted and had some limitations.
Geng Ailian etc. reported the effect of coenzyme Q10 to ascites symptoms broiler growth performance and oxidation resistance aspect (Geng Ailian etc. journal of animal science and veterinary medicine .36 (1), 16～20 (2005); Geng Ailian etc. Animal nutrition journal .18 (2), 86～92 (2006)) effect, but there is not yet any relevant coenzyme Q10 at the report of preventing and treating aspect fowl gout.
Summary of the invention
The object of the invention is to provide the purposes of coenzyme Q10 aspect preparation control fowl gout medicine.
Another object of the present invention is to provide the purposes of coenzyme Q10 pre-mixing agent aspect preparation control fowl gout medicine.This coenzyme Q10 pre-mixing agent can be used for preventing and treating fowl gout, significantly relaxes the wind-induced Pathological damage of laying hen fowl pain, and can significantly improve fowl immunity of organisms.
The present invention's the 3rd object is to provide the purposes of coenzyme Q10 aspect the wind-induced Pathological damage medicine of preparation mitigation laying hen fowl pain.
The present invention's the 4th object is to provide the purposes of coenzyme Q10 pre-mixing agent aspect the wind-induced Pathological damage medicine of preparation mitigation laying hen fowl pain.
Described fowl refers to the one of chicken, duck, chicken, turkey, aquatic bird, Columba livia or Carnis Coturnicis japonicae etc.
Constituent and the percentage by weight thereof of described coenzyme Q10 pre-mixing agent are: coenzyme Q10 2～5%, and carrier 95～98%, wherein said carrier refers to zeolite powder, Semen Maydis powder or starch.
The preparation method of described coenzyme Q10 pre-mixing agent is that coenzyme Q10 is dry, crosses 65 mesh sieves; By carrier drying, cross 50 mesh sieves; According to equivalent incremental method, coenzyme Q10 and equivalent carrier are placed in mixer, stirring and evenly mixing, obtains coenzyme Q10 pre-composition; Then to adding in mixer and the carrier of coenzyme Q10 pre-composition equivalent, stirring and evenly mixing; So increase progressively interpolation carrier, stir; In the most backward mixer, add remaining carrier, stirring and evenly mixing.
The using method of coenzyme Q10 pre-mixing agent of the present invention, using coenzyme Q10 pre-mixing agent as feed additive, mixs homogeneously with basal diet.The additive capacity of coenzyme Q10 in feedstuff is 40mg/kg feedstuff.
The advantage that the present invention has and beneficial effect: (1) coenzyme Q10 of the present invention and pre-mixing agent thereof have good prevention effect to fowl gout, can significantly relax the wind-induced Pathological damage of laying hen fowl pain, reduces ill fowl mortality rate; (2) coenzyme Q10 of the present invention and pre-mixing agent thereof can significantly improve fowl immunity of organisms, strengthen fowl body resistance against diseases; (3) coenzyme Q10 pre-mixing agent of the present invention is not containing organic principle, nontoxic to fowl poultry, there is no toxic and side effects, environmental sound; (4) coenzyme Q10 pre-mixing agent of the present invention is easy to use, can save a large amount of human and material resources; (5) coenzyme Q10 pre-mixing agent preparation method of the present invention is simple, and cost is low.
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.These embodiment are only not used in restriction the scope of protection of present invention for the present invention is described.
The preparation of embodiment 1 coenzyme Q10 pre-mixing agent
Carry out as follows:
(1) coenzyme Q10 (Agricultural University Of Nanjing's pharmacological evaluation chamber provides) is dried to water content and is no more than 3%, cross 65 mesh sieves; Zeolite powder (Ninghai County is good to be provided with Chemical Co., Ltd.) is dried to water content and is no more than 5%, crosses 50 mesh sieves.
(2) get coenzyme Q10 40g, zeolite powder 1960g.According to equivalent incremental method, first zeolite powder 40g and coenzyme Q10 40g are placed in clean mixer, be uniformly mixed 5 minutes, form coenzyme Q10 pre-composition; Then in mixer, add zeolite powder 80g, be uniformly mixed 5 minutes; Then according to equivalent incremental method, constantly mix; In the most backward mixer, add remaining zeolite powder is uniformly mixed to 15 minutes to mix homogeneously, obtain coenzyme Q10 pre-mixing agent 2000g, be encapsulated as 1kg/ bag with clean bag.
The preparation of embodiment 2 coenzyme Q10 pre-mixing agents
Carry out as follows:
(1) coenzyme Q10 is dried to water content and is no more than 3%, cross 65 mesh sieves, Semen Maydis powder is dried to water content and is no more than 5%, crosses 50 mesh sieves.
(2) get coenzyme Q10 60g, zeolite powder 1940g.According to equivalent incremental method, first Semen Maydis powder 60g and coenzyme Q10 60g are placed in clean mixer, be uniformly mixed 5 minutes, form coenzyme Q10 pre-composition; Then in mixer, add Semen Maydis powder 120g, be uniformly mixed 5 minutes; According to equivalent incremental method, constantly mix; In in the most backward mixer, add remaining Semen Maydis powder to be uniformly mixed 15 minutes to mix homogeneously, obtain coenzyme Q10 pre-mixing agent 2000g.Be encapsulated as 1kg/ bag with clean bag.
The preparation of embodiment 3 coenzyme Q10 pre-mixing agents
Carry out as follows:
(1) coenzyme Q10 is dried to water content and is no more than 3%, cross 65 mesh sieves, carrier starch is dried to water content and is no more than 5%, crosses 50 mesh sieves.
(2) get coenzyme Q10 100g, zeolite powder 1900g.According to equivalent incremental method, first starch 100g and coenzyme Q10 100g are placed in clean mixer, be uniformly mixed 5 minutes, form coenzyme Q10 pre-composition; Then in mixer, add starch 200g, be uniformly mixed 5 minutes; According to equivalent incremental method, constantly mix; In the most backward mixer, add remaining starch to be uniformly mixed 15 minutes to mix homogeneously, obtain coenzyme Q10 pre-mixing agent 2000g.Be encapsulated as 1kg/ bag with clean bag.
The protective effect research of embodiment 4 coenzyme Q10 pre-mixing agents to laying hen fowl gout kidney
Carry out as follows:
(1) Experimental agents: the coenzyme Q10 pre-mixing agent of the embodiment of the present invention 1,2,3 preparations;
(2) test method: test site is in Agricultural University Of Nanjing's pharmacology group experimental animal room.120 1 age in days Yi Sha laying hens are divided into 4 groups at random, i.e. drug test group (being numbered respectively the 1st, 2,3 groups) and the matched group of embodiment 1,2,3 preparation, carry out the feeding experiment of 60 days.First 30 days, control group fed basal diet (with reference to the preparation of NRC standard), added coenzyme Q10 pre-mixing agent in the basal diet that test group is fed, and addition is 40mg (coenzyme Q10)/kg feedstuff.Latter 30 days control group fed high-calcium, high-protein daily rations (calcium 3.85%, protein 28.73%), test group is added coenzyme Q10 pre-mixing agent in high-calcium, high-protein feedstuff, and addition is still 40mg (coenzyme Q10)/kg feedstuff.In blood sampling in 0,8,16,24 day of the high-calcium, high-protein daily ration of feeding, separation of serum, dynamic measurement is observed the variation of uric acid in serum, creatinine, blood urea nitrogen.During off-test, each group is cutd open 6 chickens of inspection, does the microscopic examination of kidney.
(3) result of the test:
1, clinical observation and cut open inspection result
Matched group, in the typical fowl gout symptom that occurs for the 8th day of the high-calcium, high-protein daily ration of feeding, shows as body and becomes thin, and feces is water sample, and spirit is depressed, and serious dehydration appears in sick chicken, occurs death, and mortality rate is 36.7% (11/30).Cut open the visible kidney enlargement of inspection, be piebald shape, in excess of the kidney matter, deposit whiteness, ureter is cut off adularescent electrodeposition substance.There is fowl gout symptom the 15th talent of the high-calcium, high-protein daily ration of feeding in test group minority chicken, sporadicly dead, the 1st group of mortality rate is 6.7% (2/30), and the 2nd group of mortality rate is (1/30), and the 3rd group without death.Mortality rate chicken is cutd open inspection, and visible kidney enlargement, is piebald shape, in excess of the kidney matter, deposits whiteness, and ureter is cut off adularescent electrodeposition substance.
2, clinical blood biochemical indicator dynamic measurement result
Serum creatinine, uric acid, blood urea nitrogen are the indexs of clinical reflection renal function quality.Obvious when impaired when renal function, three's concentration can raise.
The detection of dynamic result (in Table 1) of 2.1 serum creatinines
Table 1 serum creatinine level detects (n=6) (μ mol/L) result of the test
From data in table, remove 0d beyond the highest heavens, the 1st, 2,3 groups of creatinine levels are all starkly lower than matched group.This result shows to add coenzyme Q10 in feedstuff and can effectively prevent the rising of creatinine levels in fowl goat chicken.
The detection of dynamic result (in Table 2) of 2.2 serum uric acids
Table 2 serum uric acid level detects (n=6) (μ mol/L) result of the test
From data in table, remove 0d beyond the highest heavens, the 1st, 2,3 groups of uric acid levels are all starkly lower than matched group.This result shows to add coenzyme Q10 in feedstuff and can effectively prevent the rising of uric acid level in fowl goat chicken.
The detection of dynamic result (in Table 3) of 2.3 serum urea nitrogens
(mmol/L) result of the test of table 3 serum urea nitrogen horizontal detection (n=6)
From data in table, remove 0d beyond the highest heavens, the 1st, 2,3 groups of urea nitrogen levels are all starkly lower than matched group.Show in feedstuff, to add coenzyme Q10 and can effectively prevent the rising of UAN level in fowl goat chicken.
3 pathological study results
Histopathological findings demonstration, matched group nephropathy degree is the most serious, shows as the necrosis of renal cells segment-like, ballooning degeneration, renal cells swelling, and luminal stenosis is locking even, massive inflammatory cells infiltrated.1st, 2,3 groups of nephropathy degree are obviously lighter than matched group, and renal cells edema alleviates, inflammatory cell reduces, renal tubules, and it is clear that glomerule structure becomes.
The above results explanation coenzyme Q10 can effectively reduce the wind-induced death of fowl pain, can effectively relax the wind-induced Pathological damage of fowl pain simultaneously.
The contrast test of the impact of embodiment 5 coenzyme Q10 pre-mixing agents on fowl gout body's immunity
Carry out as follows:
(1) trial drug: the coenzyme Q10 pre-mixing agent of the embodiment of the present invention 1,2,3 preparations;
(2) test method: test site is in Agricultural University Of Nanjing's pharmacology group experimental animal room.1 age in days Yi Sha laying chickling 120 plumages, carry out routine immunization by laying hen immune programme for children.After weighing, be divided at random the prepared drug test group of embodiment 1,2,3 (being numbered respectively the 1st, 2,3 groups) and blank group.First 30 days, control group fed basal diet (with reference to the preparation of NRC standard), added coenzyme Q10 pre-mixing agent in the basal diet that test group is fed, and addition is 40mg (coenzyme Q10)/kg feedstuff.Latter 30 days control group fed high-calcium, high-protein daily rations (calcium 3.85%, protein 28.73%), test group is added coenzyme Q10 pre-mixing agent in high-calcium, high-protein feedstuff, and addition is still 40mg (coenzyme Q10)/kg feedstuff.Test chicken is raised in cages, free choice feeding drinking-water.When the 8th day (chicken 39 ages in days) of the high-calcium, high-protein feedstuff of feeding, randomly draw 6 for every group, only, anticoagulant heparin, measures lymphopoiesis to heart blood sampling 2mL/.During respectively at test 46,60 age in days, get at random 6 chickens, carry out Immune Organs Index mensuration for every group.Heavy (the g)/body weight of Immune Organs Index=immune organ (kg).
Adopt mtt assay to carry out lymphocyte proliferation mensuration.By 1 times of anticoagulant blood sample Hanks ' liquid dilution, be carefully added in lymphocyte separation medium upper strata, the centrifugal 20min of 2000rpm/min, cloud leukocytic cream in the middle of drawing, washes 2 times the centrifugal 10min of 1500rpm/min with serum-free RPMI1640 nutritional solution.After viable count, with RPMI1640 culture fluid, adjusting cell concentration is 5 × 106/mL, join in 96 porocyte culture plates, 80 μ L/ holes, every hole adds 20 μ L PHA (final concentration is 20 μ g/mL) again, then be placed in 37 ℃, under 5%CO2 condition, cultivate 44h, take out every hole and add MTT20 μ L, continue to cultivate after 4h, suck supernatant, every hole adds DMSO100 μ L, cell plates are placed in and on micro-oscillator, shake 5min precipitation is dissolved completely, with detect its absorbance (A570 value) on enzyme linked immunological instrument at 570nm place, as the lymphopoietic index of T.
(3) result of the test:
1, the result of the test of the impact of peripheral blood lymphocyte proliferation (in Table 4)
Table 4 peripheral blood lymphocyte proliferation (A
570value) result of the test of impact
| Group | 39 ages in days |
| Matched group | 0.061±0.012 |
| The 1st group | 0.107±0.005 |
| The 2nd group | 0.144±0.021 |
| The 3rd group | 0.193±0.013 |
From data in table, the 1st, 2,3 groups of T lymphocyte proliferation rates, apparently higher than matched group, illustrate that in feedstuff, adding coenzyme Q10 can obviously promote the lymphocytic propagation of T.
2, the result of the test of the impact on Immune Organs Index (in Table 5)
The result of the test of table 5 on the impact of different days laying hen Immune Organs Index
From data in table, the 1st, 2,3 groups of different days thymus indexs, index and spleen index and fabricius bursa indexes are all apparently higher than matched group.
The above results shows, adds coenzyme Q10 and can significantly strengthen fowl gout laying hen lymphopoiesis in feedstuff, can promote the growth promoter of the immune organs such as thymus, spleen, fabricius bursa, thereby strengthens laying hen immunologic function.
Claims (4)
1. the purposes of coenzyme Q10 aspect preparation control fowl gout medicine.
2. the purposes of coenzyme Q10 pre-mixing agent aspect preparation control fowl gout medicine; The constituent and the percentage by weight thereof that it is characterized in that described coenzyme Q10 pre-mixing agent are: coenzyme Q10 2～5%, carrier 95～98%; The preparation method of described coenzyme Q10 pre-mixing agent is that coenzyme Q10 is dry, crosses 65 mesh sieves; By carrier drying, cross 50 mesh sieves; According to equivalent incremental method, coenzyme Q10 and equivalent carrier are placed in mixer, stirring and evenly mixing, obtains coenzyme Q10 pre-composition; Then to adding in mixer and the carrier of coenzyme Q10 pre-composition equivalent, stirring and evenly mixing; So increase progressively interpolation carrier, stir; In the most backward mixer, add remaining carrier, stirring and evenly mixing; Wherein said carrier refers to zeolite powder, Semen Maydis powder or starch.
3. the purposes of coenzyme Q10 aspect the wind-induced Pathological damage medicine of preparation mitigation laying hen fowl pain.
4. the purposes of coenzyme Q10 pre-mixing agent aspect the wind-induced Pathological damage medicine of preparation mitigation laying hen fowl pain; The constituent and the percentage by weight thereof that it is characterized in that described coenzyme Q10 pre-mixing agent are: coenzyme Q10 2～5%, carrier 95～98%; The preparation method of described coenzyme Q10 pre-mixing agent is that coenzyme Q10 is dry, crosses 65 mesh sieves; By carrier drying, cross 50 mesh sieves; According to equivalent incremental method, coenzyme Q10 and equivalent carrier are placed in mixer, stirring and evenly mixing, obtains coenzyme Q10 pre-composition; Then to adding in mixer and the carrier of coenzyme Q10 pre-composition equivalent, stirring and evenly mixing; So increase progressively interpolation carrier, stir; In the most backward mixer, add remaining carrier, stirring and evenly mixing; Wherein said carrier refers to zeolite powder, Semen Maydis powder or starch.
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| CN201410025463.3A CN103735539A (en) | 2011-12-12 | 2011-12-12 | Use of coenzyme Q10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511821A (en) * | 2019-01-10 | 2019-03-26 | 杨婧芳 | A kind of prevention and treatment bird flu feed addictive and preparation method thereof |
-
2011
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Non-Patent Citations (3)
| Title |
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| 徐彩菊等: "辅酶Q10在免疫调解中的作用，徐彩菊等，《中国卫生检验杂质》，2007年2月，第17卷第2期，第222-224页", 《中国卫生检验杂质》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109511821A (en) * | 2019-01-10 | 2019-03-26 | 杨婧芳 | A kind of prevention and treatment bird flu feed addictive and preparation method thereof |
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Application publication date: 20140423 |