CN103728271A - SPR (Surface Plasma Resonance) imaging detection device and light source thereof - Google Patents
SPR (Surface Plasma Resonance) imaging detection device and light source thereof Download PDFInfo
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Abstract
The embodiment of the invention discloses a light source of an SPR (Surface Plasma Resonance) imaging detection device. The light source comprises a white LED for generating white emergent light, a collimator for collimating the emergent light of the white LED, a polarizer for adjusting the polarization direction of the collimated emergent light to TM polarization, an attenuator for adjusting the intensity of the emergent light of the polarizer, and a filter for filtering the emergent light of the attenuator. The technical scheme provided by the embodiment of the invention is that the white LED is used for generating white emergent light, and then the filter is used for filtering the emergent light of the white LED, so that the emergent light with single wavelength can be formed, and a semiconductor optical maser in the prior art can be replaced to generate the emergent light with single wavelength. As the cost of a high-power white LED is far less than that of a high-power semiconductor optical maser, the cost of the light source in the SPR imaging detection device is reduced, and further the cost of the SPR imaging detection device is reduced.
Description
Technical field
The present invention relates to detection technique field, relate in particular to a kind of light source of surface plasma resonance image-forming pick-up unit and comprise the surface plasma resonance image-forming pick-up unit of this light source.
Background technology
Surface plasma body resonant vibration (Surface Plasmon Resonance, referred to as SPR) sensor is a kind of sensor of optical property (as dielectric thickness, the refractive index etc.) slight change that can detect testing medium, its core component is metal function layer.As certain incident angle, wavelength and horizontal magnetic (transverse magnetic, referred to as TM) incident light of polarization direction, after the coupling mechanisms such as prism, the interface excitation surface plasma-wave (Surface Plasmon Wave, referred to as SPW) of meeting between metal function layer and testing medium.When this surface plasma-wave launching efficiency is maximum, will produce SPR phenomenon, be that boundary reflection light intensity produces sharp-decay or polarization direction changes, thereby by detecting the variation of corresponding incident angle, incident wavelength, polarization direction or boundary reflection light intensity of SPR phenomenon, realize the detection to the optical property of near interface testing medium between metal function layer and testing medium.
The eighties in 20th century, spr sensor just starts for detection of near the variation of refractive index metal surface (Gordon, J.G.; Ernst, S.Surf.Sci.1980,101,499).Spr sensor was all widely used in fields such as drug screening, Biochemical Research in the last few years, become at present the important tool (Homola of qualitative and quantitative analysis biomolecular reaction (as kinetic parameter and detection substrate concentration), Chem.Rev.2008,108,462-493).
In prior art, utilize SPR principle, the method that detects near the variations in refractive index in metal surface has four kinds, comprising: angle detection, phase-detection, wavelength detect and intensity detection.Wherein, strength detecting method is that incident angle is fixed near intensity of reflected light minimum value, when the refractive index of check point generation biochemical reaction or check point changes, angle corresponding to intensity of reflected light minimum value changes, drive the variation of fixing incident angle place intensity of illumination simultaneously, thereby by detecting fixed angle place, the variation of intensity of reflected light, obtain the information of biochemical reaction or variations in refractive index, be widely used in high-throughout SPR imaging and detect.But SPR imaging detection device cost is higher in prior art.
Summary of the invention
For solving the problems of the technologies described above, the embodiment of the present invention provides a kind of light source of surface plasma resonance image-forming pick-up unit and has comprised the surface plasma resonance image-forming pick-up unit of this light source, to reduce the cost of described SPR imaging detection device.
For addressing the above problem, the embodiment of the present invention provides following technical scheme:
A light source for surface plasma resonance image-forming pick-up unit, comprising:
Produce the White LED of white emergent light;
The collimating apparatus that the emergent light of described White LED is collimated;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
The emergent light of described attenuator is carried out to the wave filter of filtering.
Preferably, described collimating apparatus is simple lens.
Preferably, described wave filter is filter plate.
A kind of surface plasma resonance image-forming pick-up unit, comprising:
Pedestal;
Level is fixed on the optical table of upper end, described pedestal side;
Be fixed on the equilateral triangle optical prism of described optical table side, one jiao of described optical prism vertically downward, is positioned on the vertical centering control axis of described optical prism;
The synchronous scanning device that is positioned at described optical prism below, described synchronous scanning device comprises: motor; Be positioned at the slide block guide rail of described motor top; Be connected with described motor, under the drive of described motor, the slide block moving up and down along described slide block guide rail; Be connected with described slide block, under the drive of described slide block, being parallel to the connection rod set of moving in described base plane; The incident arm being connected with described connection rod set one end and the reflection arm being connected with the described connection rod set other end, wherein, described incident arm and reflection arm are relatively arranged on the both sides of described optical prism;
Be fixed on the light source on described incident arm, described light source is the light source described in claim 1-3 any one;
Be fixed on the image received device on described reflection arm.
Preferably, described connection rod set comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, described first connecting rod and second connecting rod form V-type bar, and the other end of described first connecting rod is connected with incident arm, the other end of described second connecting rod with reflect arm and be connected.
Preferably, described connection rod set comprises:
The first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, described first connecting rod and second connecting rod form reverse V-shaped bar;
With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar;
The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar;
With the arc guide rail that the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod is slidably connected, the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod keeps symmetrical along described arc guide rail and slides.
Preferably, described optical table and described optical prism seal by annulus.
Preferably, the position being fixedly connected with described optical prism on described optical table is provided with groove.
Preferably, on described pedestal, be provided with the tommy of calibration described optical prism position.
Preferably, described motor is stepper motor.
Compared with prior art, technique scheme has the following advantages:
In the technical scheme that the embodiment of the present invention provides, described light source adopts White LED to produce white emergent light, and then utilize wave filter to filter the emergent light of described White LED, the emergent light that forms single wavelength, replaces semiconductor laser of the prior art to produce the coloured light that of single wavelength.Because the cost of high-power White LED will be much smaller than the cost of high power semiconductor lasers, thereby reduced described in the embodiment of the present invention cost of light source in surface plasma resonance image-forming pick-up unit, and then reduced the cost of described surface plasma resonance image-forming pick-up unit.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation of SPR imaging detection device in prior art;
The structural representation of the light source of the surface plasma resonance image-forming pick-up unit that Fig. 2 provides for the embodiment of the present invention;
The structural representation that Fig. 3 is the surface plasma resonance image-forming pick-up unit that provides in one embodiment of the invention;
The cut-open view of the partial structurtes that Fig. 4 is the surface plasma resonance image-forming pick-up unit that provides in one embodiment of the invention;
The partial structurtes schematic diagram of the surface plasma resonance image-forming pick-up unit that Fig. 5 provides for the embodiment of the present invention;
The structural representation that Fig. 6 is the surface plasma resonance image-forming pick-up unit that provides in another embodiment of the present invention.
Embodiment
As shown in Figure 1, SPR imaging detection device of the prior art, comprise: surface plasma body resonant vibration microarray Protein sensor, incident arm, reflection arm and signal processing unit, wherein, surface plasma body resonant vibration microarray Protein sensor comprises: prism 025, be placed in the micro-array chip 027 of prism 025 bottom surface and be placed in the sample pool 029 under described micro-array chip 027; Between prism 025 and micro-array chip 027, scribble refractive index oil reservoir 026; Described incident arm is positioned at a side of surface plasma body resonant vibration microarray Protein sensor, comprises successively collimating apparatus 021, the polarizer 022, attenuator 023 and the rectangular light hurdle 024 of semiconductor laser 019, built-in small filter 020; Described reflection arm is positioned at the opposite side of the surface plasma body resonant vibration microarray Protein sensor corresponding with incident arm, comprises successively lens 030 and CCD receiver 031; Described signal processing unit comprises the signal processing circuit 032 that is connected with described CCD receiver 031 and the computing machine 033 of storage signal handling procedure, and described signal processing circuit 032 is directly inserted in the host slot of computing machine 033.
During the work of the imaging detection device of SPR shown in Fig. 1, described semiconductor laser 019 produces the emergent light of single wavelength; Described collimating apparatus 021, to for the emergent light of described semiconductor laser 019 is collimated, guarantees that the incident angle of entire light is consistent; The described polarizer 022 is for being adjusted to TM polarization by the polarization direction of the emergent light after collimation; Described attenuator 023, for controlling emergent light intensity, prevents that saturated phenomenon from appearring in CCD receiver 031 when receiving reflected light; Described rectangular light hurdle 024, for the emergent light center intensity uniform parts through attenuator 023 is intercepted, guarantees the light intensity of entrance prism 025, consistent within the scope of covering space; Described lens 030 are for collecting the reflected light being produced by prism 025, refractive index oil reservoir 026 and micro-array chip 027; Described CCD receiver 031, for the emergent light of lens 030 is carried out to opto-electronic conversion, is embodied as the collection of picture detection signal.
But above-mentioned SPR imaging detection device cost is higher.
Inventor studies discovery, this is due to by the process of strength detecting method and imaging detection method combination, need to adopt suitable optical system, make incident light within the scope of institute's covering space, keep the high uniformity of incident angle, polarization direction and intensity, thereby realize under identical incident light condition, to the intensity detection of arbitrary region within the scope of covering space, and then reach the effect that imaging detects.And the monochromaticity of laser is relatively good, can directly produce the emergent light of single wavelength, be conducive to guarantee, in spatial dimension that incident light covers at it, keep the high uniformity of incident intensity, and the relatively good tune of light path.Therefore, the light source in SPR imaging detection device of the prior art adopts the light source of semiconductor laser as described SPR imaging detection device.
But, because described SPR imaging detection device is when working, require within the scope of incident light institute covering space, CCD receiver can receive the signal that can detect, thereby the emergent light that needs semiconductor laser has larger optical intensity density, described semiconductor laser can be exported larger power.And the output power of described semiconductor laser is larger, cost is higher, thereby causes the cost of SPR imaging detection device in prior art higher.
And, because the beam divergence angle of semiconductor laser is less, be difficult to produce larger illumination range, the coverage that is its emergent light is less, therefore, needs to comprise multiple lens in described collimating apparatus, emergent light to described semiconductor laser first expands, expand the coverage of described emergent light, then collimate, guarantee that incident angle is consistent.But, because the spectral distribution of laser is Gaussian distribution, after the emergent light of described semiconductor laser is expanded, can produce comparatively significantly intensity of illumination phenomenon pockety, be that the marginal portion of described emergent light and the core deviation of described emergent light are larger, therefore, need rectangular light hurdle to intercept in outgoing beam, the part of center intensity distribution uniform, thereby cause in prior art in SPR imaging detection device the size of light path system and regulate difficulty larger, and then causing the comparatively difficulty of realization of SPR imaging detection device in prior art.
In view of this, the embodiment of the present invention provides a kind of light source of surface plasma resonance image-forming pick-up unit, comprising:
Produce the White LED of white emergent light;
The collimating apparatus that the emergent light of described White LED is collimated;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
The emergent light of described attenuator is carried out to the wave filter of filtering.
Accordingly, the embodiment of the present invention also provides a kind of surface plasma resonance image-forming pick-up unit, comprising:
Pedestal;
Level is fixed on the optical table of upper end, described pedestal side;
Be fixed on the equilateral triangle optical prism of described optical table side, one jiao of described optical prism vertically downward, is positioned on the vertical centering control axis of described optical prism;
The synchronous scanning device that is positioned at described optical prism below, described synchronous scanning device comprises: motor; Be positioned at the slide block guide rail of described motor top; Be connected with described motor, under the drive of described motor, the slide block moving up and down along described slide block guide rail; Be connected with described slide block, under the drive of described slide block, being parallel to the connection rod set of moving in described base plane; The incident arm being connected with described connection rod set one end and the reflection arm being connected with the described connection rod set other end, wherein, described incident arm and reflection arm are relatively arranged on the both sides of described optical prism;
Be fixed on the light source on described incident arm, the light source of described light source for providing in the embodiment of the present invention;
Be fixed on the image received device on described reflection arm.
The surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides and comprising in the surface plasma resonance image-forming pick-up unit of this light source, its light source adopts White LED to produce white emergent light, and then utilize wave filter to filter the emergent light of described White LED, the emergent light that forms single wavelength, replaces semiconductor laser of the prior art to produce the coloured light that of single wavelength.Because the cost of high-power White LED will be much smaller than the cost of high power semiconductor lasers, thereby the cost of light source in the surface plasma resonance image-forming pick-up unit providing in the embodiment of the present invention has been provided, and then has reduced the cost of described surface plasma resonance image-forming pick-up unit.
For above-mentioned purpose of the present invention, feature and advantage can more be become apparent, below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.
Set forth detail in the following description so that fully understand the present invention.But the present invention can be different from alternate manner described here and implements with multiple, and those skilled in the art can do similar popularization without prejudice to intension of the present invention in the situation that.Therefore the present invention is not subject to the restriction of following public concrete enforcement.
Surface plasma-wave Surface Plasmon Wave, referred to as SPW) be to be formed by the collective oscillation of metal surface electric charge, and the vibration mode of propagating along metal and dielectric interface, it is present on the medium interface of two kinds of dielectric constants contrary (being generally metal and dielectric), and propagates along metal surface.And in collimated light beam, perpendicular to the wave vector component (TM pattern) of metal and dielectric surface, can excite the electronic oscillation of metal surface, form evanescent wave.
Again because by the process of strength detecting method and imaging detection method combination, need to adopt suitable optical system, make incident light within the scope of institute's covering space, keep the high uniformity of incident angle, polarization direction and intensity, thereby realize under identical incident light condition, to the intensity detection of arbitrary region within the scope of covering space, and then reach the effect that imaging detects.
Therefore the light source of the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides, as shown in Figure 2, comprising: White LED 51, collimating apparatus 52, the polarizer 53, attenuator 54 and wave filter 55.
The incident direction of each light and wavelength incomplete same in the emergent light producing due to White LED 51, and light source requirements in surface plasma resonance image-forming pick-up unit is within the scope of its light beam covering space, keep the high uniformity of incident angle, polarization direction and intensity, therefore, the light source that the embodiment of the present invention provides is when work, when described White LED 51 produces after emergent light, need to first through collimating apparatus 52, collimate, guarantee the consistent of described emergent light incident angle; The recycling polarizer 53 is adjusted to TM polarization by the polarization direction of the emergent light after collimation, so that the plasma of metal surface in excitating surface plasma resonance image-forming pick-up unit forms evanescent wave; Then, utilize attenuator 54 to regulate the intensity of illumination of the described polarizer 53 emergent lights, to prevent that image received device in surface plasma resonance image-forming pick-up unit from, when receiving reflected light, occurring saturated phenomenon; Finally, utilize wave filter 55 to carry out filtering to the emergent light of described attenuator 54, forming incident direction consistent, and thering is the emergent light of single wavelength.
Because the cost of high-power White LED 51 will be much smaller than the cost of high power semiconductor lasers, therefore the light source cost of the surface plasma resonance image-forming pick-up unit providing in the embodiment of the present invention is lower, thereby reduced the cost of described surface plasma resonance image-forming pick-up unit.
In one embodiment of the invention, described wave filter 55 can be filter plate, but the present invention do not limit this, as long as can play filter effect, obtains the emergent light of single wavelength.
It should be noted that, because the illumination range of White LED 51 emergent lights is larger, therefore, in one embodiment of the invention, described collimating apparatus 52 can be a simple lens, the emergent light of described White LED 51 is become to directional light, thereby the incident angle that guarantees described emergent light is consistent, and without the multiple lens of recycling, the emergent light of described White LED 51 is expanded, thereby simplified the light-source structure of described surface plasma resonance image-forming pick-up unit, also reduced the difficulty of the optical path adjusting in described light source.
Also it should be noted that, because the spectral distribution of the emergent light of White LED 51 is comparatively even, the intensity of illumination that is the emergent light of described White LED 51 distributes comparatively even, therefore, in one embodiment of the invention, described light source can not comprise rectangular light hurdle, thereby has further simplified the light-source structure of described surface plasma resonance image-forming pick-up unit, has reduced the difficulty of the optical path adjusting in described light source.
As shown in Figure 3, the embodiment of the present invention also provides a kind of surface plasma resonance image-forming pick-up unit, comprising: pedestal 1, optical table 2, optical prism 3, synchronous scanning device 4, light source 5 and image received device 6.
In one embodiment of the invention, described pedestal 1 is vertically placed, and its upper end, side level is fixed with optical table 2.The side of described optical table 2 is fixed with optical prism 3, seals between the two by annulus.Described optical prism 3 is equilateral triangle optical prism, and one angle vertically downward, is positioned on the vertical centering control axis of described optical prism 3.Under described optical prism 3, be provided with synchronous scanning device 4, described synchronous scanning device 4 comprises: motor 41; Be positioned at the slide block guide rail 42 of described motor 41 tops, described slide block guide rail 42 bottoms are connected with described motor 41, and described slide block guide rail 42 is fixed along the vertical centering control axis of described pedestal 1; Be connected with described motor 41, under the drive of described motor 41, the slide block 43 moving up and down along described slide block guide rail 42; Be connected with described slide block 43, under the drive of described slide block 43, in the connection rod set 44 that is parallel to described pedestal 1 flat in-plane moving; The incident arm 45 being connected with described connection rod set 44 one end and the reflection arm 46 being connected with described connection rod set 44 other ends, wherein, described incident arm 45 and reflection arm 46 are relatively arranged on the both sides of described optical prism 3.The light source 5 providing in above-mentioned arbitrary embodiment is provided on described incident arm 45; On described reflection arm 46, be provided with image received device 6.
As shown in Figure 4, in one embodiment of the invention, the position being fixedly connected with described optical prism 3 on described optical table 2 is provided with groove 21, so that the installation of described annulus 22 can also be played the effect that prevents fluid seepage.In another embodiment of the present invention, on described pedestal 1, be also provided with tommy 7, so that fixing described optical prism 3 is calibrated two of described optical prism 3 lower surfaces.Of the present invention, have in an embodiment, described motor 41 is preferably stepper motor, and described image collecting device is preferably CCD receiver.
In addition, as shown in Figure 5, the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides also comprises: be positioned at the micro-array chip 8 of plane in described optical prism 3 levels, and be positioned at micro-measuring cell 9 on described micro-array chip 8 surfaces.Preferably, in one embodiment of the invention, fill with index-matching fluid the clearance between described optical prism 3 and micro-array chip 8.
During described surface plasma resonance image-forming pick-up unit work, by described motor 41, drive described slide block 43 to move up and down along described slide block guide rail 42, thereby drive the connection rod set 44 being connected with described slide block 43, be parallel to the flat in-plane moving of described pedestal 1, and then the incident arm 45 that is connected with described connection rod set 44 one end of drive and the reflection arm 46 being connected with described connection rod set 44 other ends rotate centered by the bottom surface of described optical prism 3.Again because be provided with light source 5 on described incident arm 45, on described reflection arm 46, be provided with image received device 6, when described incident arm 45 rotates centered by the bottom surface of described optical prism 3 with reflection arm 46, the rotation that can make described light source 5 and image received device 6 produce symmetry angle, guarantee that emergent light that described light source 5 sends is with different incident angles, from the left tilted surface of described optical prism 3, inject, the interface of optical prism 3 and described micro-array chip described in directive, in described optical prism 3 and the interface of described micro-array chip, reflect, forming reflected light penetrates from the right tilted surface of described optical prism 3, full illumination is in the entrance port of described image collecting device, by described image collecting device, received, carry out imaging detection.
In this embodiment, the course of work of described light source 5 comprises: described White LED 51 produces white emergent light, first through described collimating apparatus 52, collimate, then by the described polarizer 53, described emergent light is adjusted to TM polarization, recycles described attenuator 54, regulate the light intensity of described emergent light to image received device 6 undersaturated conditions, finally utilize described wave filter 55, by the wavelength regulation of described emergent light to 600nm.Now, the emergent light of described light source 5 is injected through the left tilted surface of described optical prism 3, during the interface of optical prism 3 and micro-array chip, can cover described micro-array chip surface certain space scope described in directive.
In one embodiment of the invention, as shown in Figure 3, described connection rod set 44 comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block 43 respectively, described first connecting rod and second connecting rod form V-type bar, and the other end of described first connecting rod is connected with incident arm 45, the other end of described second connecting rod is connected with reflection arm 46.Wherein, the tie point of described first connecting rod and second connecting rod is positioned on the vertical centering control axis of described optical prism 3, and the intersection point of described incident arm 45 and reflection arm 46 place straight lines is also positioned on the vertical centering control axis of described optical prism 3.In this embodiment, described slide block 43 can be connected with described motor 41 by screw rod 47, and described motor 41 drives described screw rod 47 to be rotated, and by described screw rod 47, rotarily drives described slide block 43 and move up and down.
Preferably, described first connecting rod, second connecting rod, incident arm 45 and reflection arm 46 are isometric, thereby can calculate according to the mechanical drive advance amount of motor 41 swing offset of described screw rod 47, by the swing offset of described screw rod 47, calculate the angular displacement between described incident arm 45 and reflection arm 46 again, finally, according to the angular displacement between described incident arm 45 and reflection arm 46, calculate the variation of described light source 5 emergent light incident angles.
It should be noted that, described incident arm 45 is camber line with tie point and the reflection arm 46 of first connecting rod with the movement locus of the tie point of second connecting rod, and the movement locus of described slide block 43 is straight line, in this embodiment, while calculating the variation of described light source 5 emergent light incident angles, need first to convert the mechanical drive advance amount of described motor 41 to screw rod 47 amounts of spin, and then the amount of spin of described screw rod 47 is converted to the angular turn amount of connection rod set 44, easy like this in long-term use procedure because wearing and tearing and the dislocation of screw rod 47 produce return difference.And be subject to the impact of external shock, in the rotation process of described connection rod set 44, can not guarantee to be only positioned at the plane that is parallel to described pedestal 1 completely, also may in the plane perpendicular to described pedestal 1, rotate, cause introducing error when calculating the variation of described light source 5 emergent light incident angles.
In another embodiment of the present invention, in order further to improve the computational accuracy of the variable quantity of described light source 5 emergent light incident angles, as shown in Figure 6, described connection rod set 44 comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block 43 respectively, and described first connecting rod and second connecting rod form reverse V-shaped bar; With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar; The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar; The arc guide rail 48 being slidably connected with the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod.Wherein, described arc guide rail 48 is fixed on described pedestal 1, and its center of arc is positioned at the bottom center place of described optical prism 3; The tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod keeps symmetrical along described arc guide rail 48 and slides.
Preferably, described first connecting rod, second connecting rod, third connecting rod and the 4th connecting rod are isometric.In this embodiment can be by the mechanical drive advance amount of described motor 41, directly calculate in described connection rod set 44 first connecting rod and the tie point of third connecting rod and the tie point of second connecting rod and the 4th connecting rod along slide displacement on arc guide rail 48, again according to described slide displacement, the angular displacement of calculating described incident arm 45 and reflection arm 46, can obtain the variation of described light source 5 emergent light incident angles.
As can be seen here, when utilizing the surface plasma resonance image-forming pick-up unit providing in the present embodiment to detect, save and converted the mechanical drive advance amount of described motor 41 to screw rod 47 amounts of spin, and then the amount of spin of described screw rod 47 is converted to the computation process of the angular turn amount of connection rod set 44, thereby avoided wearing and tearing and the dislocation due to screw rod 47 in long-term use procedure to produce return difference.And, in embodiments of the present invention, because described connection rod set 44 is slidably connected with described arc guide rail 48, thereby the slip of described connection rod set 44 can be fixed in the plane at described arc guide rail 48 places, prevent that described connection rod set 44 is perpendicular to described arc guide rail 48 place rotation with in surfaces, thereby further improved the precision when calculating described light source 5 emergent light incident angles and changing.
In sum, the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides, its light source 5 adopts White LED 51 to produce white emergent light, and then utilize wave filter 55 to filter the emergent light of described White LED 51, the emergent light that forms single wavelength, replaces semiconductor laser of the prior art to produce the coloured light that of single wavelength.Because the cost of high-power White LED 51 will be much smaller than the cost of high power semiconductor lasers, thereby reduced the cost of light source 5 in the surface plasma resonance image-forming pick-up unit providing in the embodiment of the present invention, and then reduced the cost of described surface plasma resonance image-forming pick-up unit
And, the surface plasma resonance image-forming pick-up unit that the embodiment of the present invention provides, compared to surface plasma resonance image-forming pick-up unit of the prior art, size is less, optical path adjusting complexity is lower, and the computational accuracy of its light source 5 emergent light incident angles is higher.
In this instructions, various piece adopts the mode of going forward one by one to describe, and each part stresses is and the difference of other parts, between various piece identical similar part mutually referring to.
To the above-mentioned explanation of the disclosed embodiments, make professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to embodiment illustrated herein, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (10)
1. a light source for surface plasma resonance image-forming pick-up unit, is characterized in that, comprising:
Produce the White LED of white emergent light;
The collimating apparatus that the emergent light of described White LED is collimated;
The polarization direction of the emergent light after collimation is adjusted to the polarizer of TM polarization;
Regulate the attenuator of the emergent light light intensity of the described polarizer;
The emergent light of described attenuator is carried out to the wave filter of filtering.
2. light source according to claim 1, is characterized in that, described collimating apparatus is simple lens.
3. light source according to claim 1, is characterized in that, described wave filter is filter plate.
4. a surface plasma resonance image-forming pick-up unit, is characterized in that, comprising:
Pedestal;
Level is fixed on the optical table of upper end, described pedestal side;
Be fixed on the equilateral triangle optical prism of described optical table side, one jiao of described optical prism vertically downward, is positioned on the vertical centering control axis of described optical prism;
The synchronous scanning device that is positioned at described optical prism below, described synchronous scanning device comprises: motor; Be positioned at the slide block guide rail of described motor top; Be connected with described motor, under the drive of described motor, the slide block moving up and down along described slide block guide rail; Be connected with described slide block, under the drive of described slide block, being parallel to the connection rod set of moving in described base plane; The incident arm being connected with described connection rod set one end and the reflection arm being connected with the described connection rod set other end, wherein, described incident arm and reflection arm are relatively arranged on the both sides of described optical prism;
Be fixed on the light source on described incident arm, described light source is the light source described in claim 1-3 any one;
Be fixed on the image received device on described reflection arm.
5. imaging detection device according to claim 4, it is characterized in that, described connection rod set comprises: the first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, described first connecting rod and second connecting rod form V-type bar, and the other end of described first connecting rod is connected with incident arm, the other end of described second connecting rod is connected with reflection arm.
6. imaging detection device according to claim 4, is characterized in that, described connection rod set comprises:
The first connecting rod and the second connecting rod that are fixedly connected with described slide block respectively, described first connecting rod and second connecting rod form reverse V-shaped bar;
With the third connecting rod that the described first connecting rod other end is fixedly connected with, described first connecting rod and third connecting rod form V-type bar;
The 4th connecting rod being fixedly connected with the described second connecting rod other end, described second connecting rod and the 4th connecting rod form V-type bar;
With the arc guide rail that the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod is slidably connected, the tie point of tie point, second connecting rod and the 4th connecting rod of described first connecting rod and third connecting rod keeps symmetrical along described arc guide rail and slides.
7. imaging detection device according to claim 4, is characterized in that, described optical table and described optical prism seal by annulus.
8. imaging detection device according to claim 7, is characterized in that, the position being fixedly connected with described optical prism on described optical table is provided with groove.
9. imaging detection device according to claim 4, is characterized in that, is provided with the tommy of calibration described optical prism position on described pedestal.
10. imaging detection device according to claim 4, is characterized in that, described motor is stepper motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310673588.2A CN103728271B (en) | 2013-12-11 | 2013-12-11 | Surface plasma resonance image-forming detection device and light source thereof |
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