CN103725639A - Medium formula for improving heat resistance of strains - Google Patents
Medium formula for improving heat resistance of strains Download PDFInfo
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- CN103725639A CN103725639A CN201310681355.7A CN201310681355A CN103725639A CN 103725639 A CN103725639 A CN 103725639A CN 201310681355 A CN201310681355 A CN 201310681355A CN 103725639 A CN103725639 A CN 103725639A
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- medium
- strains
- bacterial strain
- heat resistance
- formula
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Abstract
The invention discloses a medium formula for improving heat resistance of strains. The formula of 1000 ml medium medium formula comprises the following components: 10 g of yeast extract, 25 g of sodium chloride, 5 g of calcium chloride, 5 g of zinc chloride, 200 g of glycerin, 20 g of isoprene, 10 g of proline, 10 g of arginine, 5 g of superoxide dismutase, and distilled water in balance amount; the pH value is adjusted to be 7.0. All components in the medium are processed to prepare the medium according to the ratio, strains are inoculated in the medium, and heating domestication is performed for a period of time, so that the heat resistance of the strains can be improved.
Description
Technical field
The present invention relates to the stable on heating culture medium prescription of a kind of raising bacterial strain.
Background technology
The substratum that researchist uses at present, just for bacterial strain can be survived therein, still but cannot change the attribute of bacterial strain itself, such as the thermotolerance of bacterial strain; Thereby bacterium cannot be played a role in high temperature, industrial production condition is caused to considerable restraint.
Summary of the invention
The object of this invention is to provide the stable on heating culture medium prescription of a kind of raising bacterial strain, this substratum can make bacterial strain in this substratum, live after for some time to possess suitable thermotolerance, and tolerate script over the temperature of self tolerance degree, thereby make bacterial strain can in high temperature, bring into play its various industrial applicability, greatly improve industrial efficiency, produced considerable economic benefit.
The technical solution adopted for the present invention to solve the technical problems is: the stable on heating culture medium prescription of a kind of raising bacterial strain, in the formula of preparation 1000ml substratum, contain: yeast extract paste 10g, sodium-chlor 25g, calcium chloride 5g, zinc chloride 5g, glycerine 200g, isoprene 20g, proline(Pro) 10g, arginine 10g, superoxide-dismutase 5g, adding distil water complements to 1000 milliliters, regulates PH to 7.0; Said components is become to substratum by formulated, inoculate into needing the bacterial strain of cultivating after for some time, can significantly improve the thermotolerance of bacterial strain.
The invention has the beneficial effects as follows: researchist can make bacterial strain live in this substratum to possess suitable thermotolerance after for some time and tolerate the temperature that originally surpasses self tolerance degree, thereby make bacterial strain can in high temperature, bring into play its various industrial applicability, greatly improve industrial efficiency, produced considerable economic benefit.
Embodiment
The stable on heating culture medium prescription of raising bacterial strain, is comprised of following component: yeast extract paste 10g, sodium-chlor 20g, calcium chloride 5g, zinc chloride 5g, glycerine 200g, isoprene 20g, proline(Pro) 10g, arginine 10g, superoxide-dismutase 5g, adding distil water complements to 1000 milliliters, regulates PH to 7.0.
Each component in substratum is mixed with to substratum in ratio in formula, inoculation is entered in this substratum, temperature raising just can improve the thermotolerance of this bacterial strain after domestication for some time gradually.
Claims (2)
1. improve the stable on heating culture medium prescription of bacterial strain, it is characterized in that: in the formula of preparation 1000ml substratum, contain:
Yeast extract paste 10g;
Sodium-chlor 25g;
Calcium chloride 5g;
Zinc chloride 5g;
Glycerine 200g;
Isoprene 20g;
Proline(Pro) 10g;
Arginine 10g;
Superoxide-dismutase 5g;
Distilled water adds to 1000ml.
2. the stable on heating culture medium prescription of raising bacterial strain according to claim 1, is characterized in that: regulate PH to 7.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310681355.7A CN103725639A (en) | 2013-12-13 | 2013-12-13 | Medium formula for improving heat resistance of strains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310681355.7A CN103725639A (en) | 2013-12-13 | 2013-12-13 | Medium formula for improving heat resistance of strains |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103725639A true CN103725639A (en) | 2014-04-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310681355.7A Pending CN103725639A (en) | 2013-12-13 | 2013-12-13 | Medium formula for improving heat resistance of strains |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103725639A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129217A (en) * | 2019-04-22 | 2019-08-16 | 华中农业大学 | A kind of method and application improving lactic acid bacteria heat resistance |
-
2013
- 2013-12-13 CN CN201310681355.7A patent/CN103725639A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129217A (en) * | 2019-04-22 | 2019-08-16 | 华中农业大学 | A kind of method and application improving lactic acid bacteria heat resistance |
| CN110129217B (en) * | 2019-04-22 | 2021-07-23 | 华中农业大学 | Method for improving heat resistance of lactic acid bacteria and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140416 |
|
| WD01 | Invention patent application deemed withdrawn after publication |