CN103724388B - A kind of method extracting plant sterol from Kidney bean - Google Patents
A kind of method extracting plant sterol from Kidney bean Download PDFInfo
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- CN103724388B CN103724388B CN201310603475.5A CN201310603475A CN103724388B CN 103724388 B CN103724388 B CN 103724388B CN 201310603475 A CN201310603475 A CN 201310603475A CN 103724388 B CN103724388 B CN 103724388B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention provides the extracting method of a plant sterols, particularly relate to a kind of method extracting plant sterol from Kidney bean, belong to technical field of natural product extraction.Comprise the steps: to gather fresh Kidney bean, after peeling, get seed, seed is ground to form fine powder; The phosphate buffer solution of preparation prozyme; Phosphate buffer soln is mixed with fine powder, carries out enzymolysis, obtain enzymolysis solution; Enzymolysis solution is filtered, obtains filter residue, filter residue is squeezed, remove excess water, obtain wet-milling; Wet-milling is dropped in extraction kettle, carry out carbon dioxide upercritical fluid extraction, then obtain solid sterol by the separation of Cyclonic separator.The present invention successfully extracts plant sterol from Kidney bean, and product yield is high, and purity is good.
Description
Technical field
The invention provides the extracting method of a plant sterols, particularly relate to a kind of method extracting plant sterol from Kidney bean, belong to technical field of natural product extraction.
Background technology
Plant sterol (phytosterol or plant sterol) is a kind of activeconstituents in plant, structurally as similar in cholesterol to animality sterol.Sterol is 3 oxy-compound of ring penta perhydro phenanthrene, C-5 has the title sterol of double bond, the title stanols that C-5 is saturated.Sterol has 4 kinds of existence forms in plant materials: free state, sterol ester (fatty acid ester and phenols acids), steroid base glucosides and acidylate steroid base glucosides free sterol and sterol ester dissolve in non-polar solvent as normal hexane, and steroid base glucosides and acidylate steroid base glucosides need polarity modifier to dissolve.Plant sterol is extensively present in the root of plant, stem, leaf, fruit and seed, and its content of different floristics is different.Vegetables oil and oleaginous food are the primary natural source of plant sterol, are secondly cereal, grain products and nut.
Plant sterol has stronger anti-inflammatory action to human body, has the effects such as the biochemistry synthesis that human body can be suppressed the absorption of cholesterol, the catabolism promoting cholesterol, suppression cholesterol.For the heart trouble of prophylactic treatment coronary atherosclerosis class, there is obvious curative effect to treatment ulcer, Skin Squamous Cell Carcinoma, cervical cancer etc.; Can wound healing be promoted, make hypertrophy, strengthen capillary circulation; Also can be used as the hold back agent that gallbladdergallstonecholetithiasis is formed.
Kidney bean formal name used at school Kidney bean (be commonly called as two season beans or string bean), pulse family section Phaseolus.Kidney bean originates in Mexico and the Argentina in America.Pod is long 10 ~ 20 centimetres, and directly or slightly bending, transverse section is circular or oblate, and epidermis is close by fine hair for shape; Tender pod is the colors such as deep mixed green, yellow, purplish red (or dapple), yellowish-white to tawny time ripe.Along with beans pod development, the vascular bundle at its back of the body, outside of belly suture place is flourishing gradually, in, the sclerenchyma number of plies of endocarp increases gradually, thus fresh food quality reduces.Therefore tender pod is gathered and will make every effort in good time.Every pod is containing 4 ~ 8, seed, and seed kidney shape, has the colors such as red, white, yellow, black and speckle; Thousand seed weight 0.3 ~ 0.7 kilogram.
Summary of the invention
The object of the invention is: provide a kind of method extracting plant sterol from Kidney bean, concrete technical scheme is as follows:
From Kidney bean, extract a method for plant sterol, comprise the steps:
1st step, gather fresh Kidney bean, after peeling, get seed, seed is ground to form fine powder;
2nd step, the pH of preparation containing mass concentration 1 ~ 4% prozyme are the phosphate buffer solution of 3.5 ~ 4.5, and described prozyme refers to the cellulase of mass ratio 2: 1: protease;
3rd step, phosphate buffer soln to be mixed according to weight ratio 15 ~ 10:1 with fine powder, be heated to 35 ~ 45 DEG C, carry out enzymolysis, obtain enzymolysis solution;
4th step, enzymolysis solution to be filtered, obtain filter residue, filter residue is squeezed, remove excess water, obtain wet-milling;
5th step, wet-milling is dropped in extraction kettle, pass into and guide and support agent and CO
2gas, the quality of guiding and supporting agent is 3% ~ 5% of wet-milling weight, and extraction conditions is: extraction temperature 40 ~ 55 DEG C, pressure 20 ~ 30MPa; Be separated a temperature 40 ~ 60 DEG C, pressure 5 ~ 10MPa; Be separated two temperature 30 ~ 40 DEG C, pressure 3 ~ 5MPa; Carbon dioxide flow is 200 ~ 220kg/h, extraction time 3 ~ 4 hours, the Cyclonic separator that fluid separation one exported is sent into, and is separated and obtains solid sterol from fluid-phase.
As preferably, the granularity of the fine powder in the 1st step is 200 order ~ 400 orders.
As preferably, described agent of guiding and supporting refers to the solution that water, ethanol, ethyl acetate mix with volume ratio 1: 3: 3.
As preferably, the pressure 5 ~ 7MPa in Cyclonic separator, temperature is 30 ~ 35 DEG C.
beneficial effect
The present invention successfully extracts plant sterol from Kidney bean, and product yield is high, and purity is good.
Embodiment
Embodiment 1
1st step, gather fresh Kidney bean, after peeling, get seed, seed is ground to form fine powder, granularity is 200 order ~ 400 orders;
2nd step, the pH of preparation containing mass concentration 1% prozyme are the phosphate buffer solution of 3.5 ~ 4.5, and described prozyme refers to the cellulase of mass ratio 2: 1: protease;
3rd step, phosphate buffer soln to be mixed according to weight ratio 15:1 with fine powder, be heated to 35 DEG C, carry out enzymolysis, obtain enzymolysis solution;
4th step, enzymolysis solution to be filtered, obtain filter residue, filter residue is squeezed, remove excess water, obtain wet-milling;
5th step, wet-milling is dropped in extraction kettle, pass into and guide and support agent and CO
2gas, the quality of guiding and supporting agent is 3% of wet-milling weight, and described agent of guiding and supporting refers to the solution that water, ethanol, ethyl acetate mix with volume ratio 1: 3: 3, and extraction conditions is: extraction temperature 40 DEG C, pressure 20MPa; Be separated a temperature 40 DEG C, pressure 5MPa; Be separated two temperature 30 DEG C, pressure 3MPa; Carbon dioxide flow is 200kg/h, extraction time 3 hours, the Cyclonic separator that fluid separation one exported is sent into, the pressure 5MPa in Cyclonic separator, and temperature is 35 DEG C, is separated and obtains solid sterol from fluid-phase.
In the Kidney bean of every 100g, can extract and obtain plant sterol 76 mg, purity is 75.2%.
Embodiment 2
1st step, gather fresh Kidney bean, after peeling, get seed, seed is ground to form fine powder, granularity is 200 order ~ 400 orders;
2nd step, the pH of preparation containing mass concentration 4% prozyme are the phosphate buffer solution of 3.5 ~ 4.5, and described prozyme refers to the cellulase of mass ratio 2: 1: protease;
3rd step, phosphate buffer soln to be mixed according to weight ratio 10:1 with fine powder, be heated to 45 DEG C, carry out enzymolysis, obtain enzymolysis solution;
4th step, enzymolysis solution to be filtered, obtain filter residue, filter residue is squeezed, remove excess water, obtain wet-milling;
5th step, wet-milling is dropped in extraction kettle, pass into and guide and support agent and CO
2gas, the quality of guiding and supporting agent is 5% of wet-milling weight, and described agent of guiding and supporting refers to the solution that water, ethanol, ethyl acetate mix with volume ratio 1: 3: 3, and extraction conditions is: extraction temperature 55 DEG C, pressure 30MPa; Be separated a temperature 60 C, pressure 10MPa; Be separated two temperature 40 DEG C, pressure 5MPa; Carbon dioxide flow is 220kg/h, extraction time 4 hours, the Cyclonic separator that fluid separation one exported is sent into, the pressure 7MPa in Cyclonic separator, and temperature is 35 DEG C, is separated and obtains solid sterol from fluid-phase.
In the Kidney bean of every 100g, can extract and obtain plant sterol 78 mg, purity is 76.4%.
Embodiment 3
1st step, gather fresh Kidney bean, after peeling, get seed, seed is ground to form fine powder, granularity is 200 order ~ 400 orders;
2nd step, the pH of preparation containing mass concentration 3% prozyme are the phosphate buffer solution of 3.5 ~ 4.5, and described prozyme refers to the cellulase of mass ratio 2: 1: protease;
3rd step, phosphate buffer soln to be mixed according to weight ratio 12:1 with fine powder, be heated to 40 DEG C, carry out enzymolysis, obtain enzymolysis solution;
4th step, enzymolysis solution to be filtered, obtain filter residue, filter residue is squeezed, remove excess water, obtain wet-milling;
5th step, wet-milling is dropped in extraction kettle, pass into and guide and support agent and CO
2gas, the quality of guiding and supporting agent is 4% of wet-milling weight, and described agent of guiding and supporting refers to the solution that water, ethanol, ethyl acetate mix with volume ratio 1: 3: 3, and extraction conditions is: extraction temperature 50 DEG C, pressure 25MPa; Be separated a temperature 45 C, pressure 8MPa; Be separated two temperature 35 DEG C, pressure 4MPa; Carbon dioxide flow is 2100kg/h, extraction time 3 hours, the Cyclonic separator that fluid separation one exported is sent into, the pressure 5MPa in Cyclonic separator, and temperature is 32 DEG C, is separated and obtains solid sterol from fluid-phase.
In the Kidney bean of every 100g, can extract and obtain plant sterol 72mg, purity is 79.5%.
Claims (1)
1. from Kidney bean, extract a method for plant sterol, it is characterized in that, comprise the steps:
1st step, gather fresh Kidney bean, after peeling, get seed, seed is ground to form fine powder, granularity is 200 order ~ 400 orders;
2nd step, the pH of preparation containing mass concentration 3% prozyme are the phosphate buffer solution of 3.5 ~ 4.5, and described prozyme refers to the cellulase of mass ratio 2: 1: protease;
3rd step, phosphate buffer soln to be mixed according to weight ratio 12:1 with fine powder, be heated to 40 DEG C, carry out enzymolysis, obtain enzymolysis solution;
4th step, enzymolysis solution to be filtered, obtain filter residue, filter residue is squeezed, remove excess water, obtain wet-milling;
5th step, wet-milling is dropped in extraction kettle, pass into and guide and support agent and CO
2gas, the quality of guiding and supporting agent is 4% of wet-milling weight, and described agent of guiding and supporting refers to the solution that water, ethanol, ethyl acetate mix with volume ratio 1: 3: 3, and extraction conditions is: extraction temperature 50 DEG C, pressure 25MPa; Be separated a temperature 45 C, pressure 8MPa; Be separated two temperature 35 DEG C, pressure 4MPa; Carbon dioxide flow is 2100kg/h, extraction time 3 hours, the Cyclonic separator that fluid separation one exported is sent into, the pressure 5MPa in Cyclonic separator, and temperature is 32 DEG C, is separated and obtains solid sterol from fluid-phase;
In the Kidney bean of every 100g, extract and obtain plant sterol 72mg, purity is 79.5%.
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| CN103724388B true CN103724388B (en) | 2015-08-19 |
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| CN105819935A (en) * | 2016-06-12 | 2016-08-03 | 句容市后白良种场 | Method for preparing tomato soilless culture substrate |
| CN106069658A (en) * | 2016-06-12 | 2016-11-09 | 句容市后白良种场 | A kind of preparation method of organic type Fructus Cucumidis sativi soilless culture substrate |
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| CN101381398A (en) * | 2008-10-30 | 2009-03-11 | 九三粮油工业集团有限公司 | Supercritical extraction method of soybean phytosterol material by CO2 |
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Effective date of registration: 20210428 Address after: 226300 WuJie central bridge, Tongzhou District, Nantong City, Jiangsu Province Patentee after: Nantong Tongzhou District WuJie agricultural electromechanical management station Address before: 226300, Jiangsu Nantong hi tech Zone, Century Road, No. 788 East Science Park, building of excellence Patentee before: NEOVOLTAIC ENERGY NANTONG Co.,Ltd. |
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