CN103720733A - Preparation method of manyprickle acanthopanax root extract - Google Patents
Preparation method of manyprickle acanthopanax root extract Download PDFInfo
- Publication number
- CN103720733A CN103720733A CN201410031670.XA CN201410031670A CN103720733A CN 103720733 A CN103720733 A CN 103720733A CN 201410031670 A CN201410031670 A CN 201410031670A CN 103720733 A CN103720733 A CN 103720733A
- Authority
- CN
- China
- Prior art keywords
- radix
- preparation
- acanthopanacis senticosi
- caulis acanthopanacis
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a preparation method of a manyprickle acanthopanax root extract. According to the preparation method, an improvement on an existing manyprickle acanthopanax root crude-extract technology is performed, and a microbial enzyme liquid processing step is added, so that the dissolution-out of active ingredients of manyprickle acanthopanax roots can be effectively increased, and the yield of manyprickle acanthopanax root crude-extract products is increased by 20-30%, thereby effectively using wild resources, greatly increasing the utilization rate of raw materials, and improving the economic benefits; the discharge capacity of decoction dregs pollutants can be reduced, therefore, effects of environmental friendliness and no pollution are achieved, and the green development of manyprickle acanthopanax roots is realized; the method disclosed by the invention is easy to operation, and suitable for industrialized production; the content of effective components of the manyprickle acanthopanax root extract is significantly improved in comparison with that in the prior art, therefore, the method has a good market prospect.
Description
Technical field
The invention belongs to the preparation method of medicinal plant extract, be specifically related to a kind of preparation method of Radix Et Caulis Acanthopanacis Senticosi extract.
Background technology
Radix Et Caulis Acanthopanacis Senticosi is Araliaceae Acanthopanax shrub, and root, stem, leaf, skin, fruit all can people's medicines.Radix Et Caulis Acanthopanacis Senticosi has the disorder of the body of adjusting, the functions such as invigorating middle warmer, benefit essence, strong will, wind-damp dispelling, strengthening bone and muscle, blood circulation and promoting silt, stomach invigorating diuresis.Radix Et Caulis Acanthopanacis Senticosi extract has the effects such as replenishing QI to invigorate the spleen, beneficial able-bodied bone, strengthening the body resistance, tranquillizing and allaying excitement, can regulate human body metabolism, strengthens body immunity.Medicine take Radix Et Caulis Acanthopanacis Senticosi as raw material and food are subject to welcome both domestic and external deeply, but recent two decades is robbed excavating of formula, and wild Radix Et Caulis Acanthopanacis Senticosi resource is seriously damaged, and it is exhausted that natural resources are tending towards; The means organic solvent consumption that obtains at present Radix Et Caulis Acanthopanacis Senticosi extract is large, has high input, seriously polluted; And partial solvent be difficult to reclaim, toxic, can cause great injury to human body; Therefore, how on the basis of current extracting method, to develop new extracting method to improve the productive rate of effective ingredient in Radix Et Caulis Acanthopanacis Senticosi, improve the utilization rate of the former medicine of Radix Et Caulis Acanthopanacis Senticosi, reduce environmental pollution, become current this area urgent problem.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of Radix Et Caulis Acanthopanacis Senticosi extract, the method can provide the preparation method that a kind of production cost is low, pollute little Radix Et Caulis Acanthopanacis Senticosi extract.
The technical solution adopted in the present invention is: a kind of preparation method of Radix Et Caulis Acanthopanacis Senticosi extract, is characterized in that: the method comprises the steps:
(1) preparation of microbial enzyme: the mixture of preparing three parts of wheat brans and water, the wheat bran that is 1:1 by mass ratio and water mix, mix thoroughly, sterilizing 60 minutes under 120 ~ 130 ℃ of conditions, the mixture of three parts of wheat brans and water is inoculated respectively to aspergillosis, Trichoderma spp. and root enzyme, at 28 ~ 32 ℃, cultivate 40-60 hour, and then to the water that adds 4 ~ 6 times of its quality in every part of postvaccinal mixture, under 28 ~ 32 ℃ of conditions, soak 2 ~ 4 hours, after filtration, get supernatant, will after the supernatant mix homogeneously of aspergillosis, Trichoderma spp. and root enzyme, obtain microbial enzyme liquid;
(2) preparation of Radix Et Caulis Acanthopanacis Senticosi raw material: Radix Et Caulis Acanthopanacis Senticosi rhizome is crushed to 80 ~ 100 orders, adds the water soaking of 4 times of Radix Et Caulis Acanthopanacis Senticosi rhizome opaque amounts, then heat 60 minutes under 80 ~ 120 ℃ of conditions;
(3) enzymolysis: treat that in step (2), Radix Et Caulis Acanthopanacis Senticosi rhizome amidin temperature is down to 30 ~ 40 ℃, add the microbial enzyme liquid of continuing to employ in step (1), be incubated enzymolysis 8 ~ 24 hours after mix homogeneously under 30 ℃ of conditions;
(4) alcohol is mentioned the preparation of extract: the mixed liquor in 85 ℃ of water-baths after the middle enzymolysis of use alcohol reflux lixiviate step (3) 6 ~ 10 times, in the mixed liquor after enzymolysis in step (3), add ethanol at every turn, the ultimate density that makes ethanol is 65 ~ 85%, the time of each lixiviate is 20 ~ 90 minutes, obtain extracting solution, by centrifugal extracting solution (1800r/min, 8min) rear with 400 order silk cover filterings, filtrate distilling under reduced pressure under vacuum 0.015Mpa is removed to ethanol, then the dry Radix Et Caulis Acanthopanacis Senticosi extract that obtains.
The microbial enzyme liquid using in the present invention derives from aspergillosis (Aspergillus), Trichoderma spp. (Trichoderma) and root enzyme (Rhizopus), what wherein in aspergillosis (Aspergillus), commonly use is aspergillus niger (Aspergillus niger) and aspergillus oryzae (Aspergillus oryzae), what in Trichoderma spp. (Trichoderma), commonly use is Trichoderma viride (Trichoderma mavirde) and trichoderma reesei (Trichoderma reesei), that in root enzyme (Rhizopus), commonly use is a day origin enzyme (Rhizopus japonicus), meegan enzyme (Rhizopus oryzae) and magnificent root enzyme (Rhizopus chinenisi).
Beneficial effect of the present invention: the present invention improves existing Radix Et Caulis Acanthopanacis Senticosi crude extract technology, add microbial enzyme liquid treatment step, can effectively increase the stripping of Radix Et Caulis Acanthopanacis Senticosi effective ingredient, the output that can make Radix Et Caulis Acanthopanacis Senticosi slightly carry product increases by 20% ~ 30%, thereby effectively utilize wild resource, greatly increase raw-material utilization rate, improved economic benefit; The present invention can reduce the discharge capacity of medicinal residues pollutant, environment friendly and pollution-free, realizes the Green Development to Radix Et Caulis Acanthopanacis Senticosi; Easy operating of the present invention simultaneously, is applicable to suitability for industrialized production; In Radix Et Caulis Acanthopanacis Senticosi extract of the present invention, the content of effective ingredient increases substantially than prior art, has fabulous market prospect.
The specific embodiment
Below in conjunction with instantiation, the invention will be further described.
A preparation method for Radix Et Caulis Acanthopanacis Senticosi extract, comprises the following steps:
(1) preparation of microbial enzyme: the mixture of preparing three parts of wheat brans and water, the wheat bran that is 1:1 by mass ratio and water mix, mix thoroughly, sterilizing 60 minutes under 130 ℃ of conditions, the mixture of three parts of wheat brans and water is inoculated respectively to aspergillosis, Trichoderma spp. and root enzyme, at 30 ℃, cultivate 60 hours, and then to the water that adds 4 times of its quality in every part of postvaccinal mixture, under 32 ℃ of conditions, soak 4 hours, after filtration, get supernatant, will after the supernatant mix homogeneously of aspergillosis, Trichoderma spp. and root enzyme, obtain microbial enzyme liquid;
(2) preparation of Radix Et Caulis Acanthopanacis Senticosi raw material: Radix Et Caulis Acanthopanacis Senticosi rhizome is crushed to 80 orders, adds the water soaking of 4 times of Radix Et Caulis Acanthopanacis Senticosi rhizome opaque amounts, then heat 60 minutes under 120 ℃ of conditions;
(3) enzymolysis: treat that in step (2), Radix Et Caulis Acanthopanacis Senticosi rhizome amidin temperature is down to 35 ℃, add the microbial enzyme liquid of continuing to employ in step (1), be incubated enzymolysis 24 hours after mix homogeneously under 30 ℃ of conditions;
(4) alcohol is mentioned the preparation of extract: the mixed liquor in 85 ℃ of water-baths after the middle enzymolysis of use alcohol reflux lixiviate step (3) 10 times, in the mixed liquor after enzymolysis in step (3), add ethanol at every turn, the ultimate density that makes ethanol is 85%, the time of each lixiviate is 90 minutes, obtain extracting solution, by after centrifugal extracting solution (1800r/min, 8min) with 400 order silk cover filterings, filtrate distilling under reduced pressure under vacuum 0.015Mpa is removed to ethanol, then the dry Radix Et Caulis Acanthopanacis Senticosi extract that obtains.
Claims (1)
1. a preparation method for Radix Et Caulis Acanthopanacis Senticosi extract, is characterized in that: the method comprises the steps:
(1) preparation of microbial enzyme: the mixture of preparing three parts of wheat brans and water, the wheat bran that is 1:1 by mass ratio and water mix, mix thoroughly, sterilizing 60 minutes under 120 ~ 130 ℃ of conditions, the mixture of three parts of wheat brans and water is inoculated respectively to aspergillosis, Trichoderma spp. and root enzyme, at 28 ~ 32 ℃, cultivate 40-60 hour, and then to the water that adds 4 ~ 6 times of its quality in every part of postvaccinal mixture, under 28 ~ 32 ℃ of conditions, soak 2 ~ 4 hours, after filtration, get supernatant, will after the supernatant mix homogeneously of aspergillosis, Trichoderma spp. and root enzyme, obtain microbial enzyme liquid;
(2) preparation of Radix Et Caulis Acanthopanacis Senticosi raw material: Radix Et Caulis Acanthopanacis Senticosi rhizome is crushed to 80 ~ 100 orders, adds the water soaking of 4 times of Radix Et Caulis Acanthopanacis Senticosi rhizome opaque amounts, then heat 60 minutes under 80 ~ 120 ℃ of conditions;
(3) enzymolysis: treat that in step (2), Radix Et Caulis Acanthopanacis Senticosi rhizome amidin temperature is down to 30 ~ 40 ℃, add the microbial enzyme liquid of continuing to employ in step (1), be incubated enzymolysis 8 ~ 24 hours after mix homogeneously under 30 ℃ of conditions;
(4) alcohol is mentioned the preparation of extract: the mixed liquor in 85 ℃ of water-baths after the middle enzymolysis of use alcohol reflux lixiviate step (3) 6 ~ 10 times, in the mixed liquor after enzymolysis in step (3), add ethanol at every turn, the ultimate density that makes ethanol is 65 ~ 85%, the time of each lixiviate is 20 ~ 90 minutes, obtain extracting solution, by centrifugal extracting solution (1800r/min, 8min) rear with 400 order silk cover filterings, filtrate distilling under reduced pressure under vacuum 0.015Mpa is removed to ethanol, then the dry Radix Et Caulis Acanthopanacis Senticosi extract that obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410031670.XA CN103720733A (en) | 2014-01-23 | 2014-01-23 | Preparation method of manyprickle acanthopanax root extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410031670.XA CN103720733A (en) | 2014-01-23 | 2014-01-23 | Preparation method of manyprickle acanthopanax root extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103720733A true CN103720733A (en) | 2014-04-16 |
Family
ID=50445195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410031670.XA Pending CN103720733A (en) | 2014-01-23 | 2014-01-23 | Preparation method of manyprickle acanthopanax root extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103720733A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100463984B1 (en) * | 2002-12-24 | 2004-12-30 | 강훈구 | Manufacturing method of drink its main material is extract of Acanthopanax senticosus Harms |
| CN101100660A (en) * | 2007-06-14 | 2008-01-09 | 东莞理工学院 | Method for producing cellulose by microorganism mixing fermentation |
| CN101967504A (en) * | 2010-06-28 | 2011-02-09 | 南京泽朗农业发展有限公司 | Method for extracting isofraxidin |
| CN102676404A (en) * | 2012-05-29 | 2012-09-19 | 黑龙江大学 | Endophytic fungi capable of improving content of isofraxidin in Radix Acanthopanacis Semticosi through fermenting Radix Acanthopanacis Semticosi |
| CN102977164A (en) * | 2012-11-05 | 2013-03-20 | 河南牧翔动物药业有限公司 | Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms |
| CN103087922A (en) * | 2012-10-29 | 2013-05-08 | 温州大学 | Penicillium, cellulose produced by fermenting penicillium in solid state and method thereof for preparing pomelo peel high-ester pectin |
-
2014
- 2014-01-23 CN CN201410031670.XA patent/CN103720733A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100463984B1 (en) * | 2002-12-24 | 2004-12-30 | 강훈구 | Manufacturing method of drink its main material is extract of Acanthopanax senticosus Harms |
| CN101100660A (en) * | 2007-06-14 | 2008-01-09 | 东莞理工学院 | Method for producing cellulose by microorganism mixing fermentation |
| CN101967504A (en) * | 2010-06-28 | 2011-02-09 | 南京泽朗农业发展有限公司 | Method for extracting isofraxidin |
| CN102676404A (en) * | 2012-05-29 | 2012-09-19 | 黑龙江大学 | Endophytic fungi capable of improving content of isofraxidin in Radix Acanthopanacis Semticosi through fermenting Radix Acanthopanacis Semticosi |
| CN103087922A (en) * | 2012-10-29 | 2013-05-08 | 温州大学 | Penicillium, cellulose produced by fermenting penicillium in solid state and method thereof for preparing pomelo peel high-ester pectin |
| CN102977164A (en) * | 2012-11-05 | 2013-03-20 | 河南牧翔动物药业有限公司 | Enzyme-assistant extraction technology of hyperin in leaves of Acanthopanax senticosus Harms |
Non-Patent Citations (3)
| Title |
|---|
| 艾斌凌等: "麸皮对里氏木霉Rut C-30产纤维素酶的促进作用", 《生物质化学工程》 * |
| 陈书峰等: "绿色木霉在稻壳和麸皮混合基质上固态发酵生产纤维素酶的研究", 《食品与发酵工业》 * |
| 鱼红闪等: "部分霉菌曲对谷物的水解性能及其酶系", 《大连轻工业学院学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103503962B (en) | Anti-aging wheat germ biscuit and preparation method thereof | |
| CN102793040B (en) | Preparation method of mulberry leaf tea | |
| CN103907713A (en) | Method for preparing waxberry black tea | |
| CN104042529A (en) | Anti-allergic cosmetic composition and preparation method thereof | |
| CN103849523B (en) | A kind of making method of Rhizome of Japanese Polypody yellow rice wine | |
| CN106085750A (en) | A kind of processing method of Rhizoma Bistortae Radix ziziphi mauritianae health promoting wine | |
| CN107586641A (en) | A kind of Qingqian Willow leaf tea wine | |
| CN103849522A (en) | Brewing process of wild buckwheat rhizome-wintersweet root healthcare wine | |
| CN104054925A (en) | Easily-digested palatable rabbit feed and preparation method thereof | |
| CN103843922A (en) | Lobed kudzuvine root slimming tea | |
| CN102742705A (en) | Sealwort sanye tea | |
| CN104306732A (en) | A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule and a preparing method thereof | |
| CN103627571A (en) | Chrysanthemum wine | |
| CN103232920A (en) | Flavor rice wine containing Fructus Tsaoko and coffee, and preparation method thereof | |
| CN103436410A (en) | Vaccinium bracteatum rice Chinese spirit and preparation method thereof | |
| CN106106928A (en) | A kind of Folium Nelumbinis Fructus Citri Limoniae alternative tea formula and production technology thereof | |
| CN103720733A (en) | Preparation method of manyprickle acanthopanax root extract | |
| CN103734678A (en) | Beef paste with healthcare function in spring and preparation method thereof | |
| CN104171543A (en) | Formula and preparation method of antiviral natural chicken feed | |
| CN103931705B (en) | A kind of wax gourd undaria pinnitafida biscuit and preparation method thereof | |
| CN108770886B (en) | Flammulina velutipes growth regulator | |
| CN103349202B (en) | Radix puerariae alcohol-dispelling corn flour and preparation method thereof | |
| CN106190690A (en) | A kind of Fructus Rubi fruit wine | |
| CN104845831A (en) | Formula and manufacturing technology for health-preserving health-care wine | |
| CN103666915A (en) | Coconut-flavored flower and plant rice wine and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140416 |