CN103719879A - Method for manufacturing laminaria japonica aresch dietary fiber - Google Patents
Method for manufacturing laminaria japonica aresch dietary fiber Download PDFInfo
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- CN103719879A CN103719879A CN201310649386.4A CN201310649386A CN103719879A CN 103719879 A CN103719879 A CN 103719879A CN 201310649386 A CN201310649386 A CN 201310649386A CN 103719879 A CN103719879 A CN 103719879A
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- dietary fiber
- laminaria japonica
- tangle
- sea
- water
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 title abstract description 5
- 241000015177 Saccharina japonica Species 0.000 title abstract 9
- 102000004190 Enzymes Human genes 0.000 claims abstract description 29
- 108090000790 Enzymes Proteins 0.000 claims abstract description 29
- 229940088598 enzyme Drugs 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000004365 Protease Substances 0.000 claims abstract description 21
- 108091005804 Peptidases Proteins 0.000 claims abstract description 20
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 20
- 150000001875 compounds Chemical class 0.000 claims abstract description 20
- 108010059892 Cellulase Proteins 0.000 claims abstract description 19
- 229940106157 cellulase Drugs 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 238000005360 mashing Methods 0.000 claims description 5
- 230000004907 flux Effects 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 3
- 230000002040 relaxant effect Effects 0.000 abstract description 3
- 238000010009 beating Methods 0.000 abstract description 2
- 238000010790 dilution Methods 0.000 abstract description 2
- 239000012895 dilution Substances 0.000 abstract description 2
- 239000002002 slurry Substances 0.000 abstract description 2
- 230000002255 enzymatic effect Effects 0.000 abstract 3
- 239000000413 hydrolysate Substances 0.000 abstract 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 230000009849 deactivation Effects 0.000 abstract 1
- 238000010612 desalination reaction Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 235000019419 proteases Nutrition 0.000 description 17
- 230000000694 effects Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 206010010774 Constipation Diseases 0.000 description 4
- 230000013872 defecation Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000002747 voluntary effect Effects 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Mycology (AREA)
- Edible Seaweed (AREA)
Abstract
The invention relates to a method for manufacturing laminaria japonica aresch dietary fiber. The method comprises the following steps: soaking dry laminaria japonica aresch taken as a raw material with water for desalination and softening, cleaning, draining water, and beating; adding water into the laminaria japonica aresch slurry for dilution, adding cellulase and compound protease double enzyme, controlling the enzymolysis condition for enzymolysis, and carrying out enzyme deactivation to obtain a laminaria japonica aresch enzymatic hydrolysate; adjusting the concentration and the pH value of the laminaria japonica aresch enzymatic hydrolysate, filtering the laminaria japonica aresch enzymatic hydrolysate with a tubular membrane, drying, and collecting a retention object to obtain the laminaria japonica aresch dietary fiber. The method is reasonable in process, simple and convenient to operate, high in operability, and easy to realize industrialized production; the laminaria japonica aresch dietary fiber manufactured according to the method is high in bowel relaxing efficacy.
Description
Technical field
The present invention relates to produce cellulosic other processes, especially a kind of preparation method of kelp dietary fiber.
Background technology
Dietary fiber is current a kind of functional food ingredient of generally acknowledging in the world, has unique trophic function in human body, and it can promote gastrointestinal peristalsis, increases the secretion of digestive juice, reduces the absorption of harmful substance; Can also integrate Dan Gu Tun, reduce the absorption of Dan Gu Tun, Gao Dan Gu Tun mass formed by blood stasis and artery sclerosis are played to prevention effect, therefore be called as the 3rd nutrient except water, protein, carbohydrate, lipid, vitamin, the large nutrient of mineral matter six, and be described as in 21 century food industry of greatest concernly, there are a kind of food additives of special health care and food modifying function.Dietary fiber is a kind of mixture of complexity, raw material sources difference, and also there is difference in the physicochemical properties that show, and the physiological activity being risen also has power.
At present, the research overwhelming majority of China's dietary fiber rests on terrestrial plant aspect, and less to marine field the Study on Resources.The nutritive value of sea-tangle is very high, is rich in algin, dietary fiber and mineral matter etc., is the good source that produces dietary fiber.
Summary of the invention
The object of this invention is to provide that a kind of technique is reasonable, easy and simple to handle, the preparation method of the kelp dietary fiber of advanced technology.
The technical solution adopted for the present invention to solve the technical problems is: a kind of preparation method of kelp dietary fiber, is characterized in that: process following process steps:
Choosing dry sea-tangle is raw material, with clear water, soak desalt, softening after, adopt flowing water to clean, draining, weighs, measures its protein content, with tissue mashing machine's processing of pulling an oar; Add its quality 5-10 water doubly to dilute kelp paste, regulate pH value 4.0~5.0, add the two enzymes of cellulase and compound protease, control temperature 40-55 ℃, enzymolysis 2-4h, described cellulase, the enzyme dosage of compound protease are the 2-5% of sea-tangle protein content: after enzymolysis, heating 90-100 ℃, enzyme 10~the 20min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 3-5%, regulate pH value 6.0-7.0, adopt again tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.1~0.2 μ m, operating temperature is controlled at 30~45 ℃, enters film pressure and is controlled at 2.5~3.0bar, goes out film pressure and is controlled at 0.8~1.0 bar; Filtered solution soluble solid content be down to after 0% or flux decline 65-70% after stop cleaning, collect trapped substance, dry, obtain kelp dietary fiber.
The present invention be dry sea-tangle raw material is soaked in water desalt, softening after, clean draining, making beating; Kelp paste adds water dilution, adds the two enzymes of cellulase and compound protease, and controlled enzymatic hydrolysis condition is carried out enzymolysis processing, and the enzyme that goes out obtains sea-tangle enzymolysis liquid; After sea-tangle enzymolysis liquid is adjusted to material concentration, pH value, adopt tubular membrane to filter, collect trapped substance, obtain kelp dietary fiber.This trapped substance is the unhydrolyzable part after water extraction and enzymolysis and extraction, is rich in crude fibre, has unique local flavor and the effect relaxing bowel.In the enzymolysis process of sea-tangle slurries of the present invention, be to utilize cellulase effectively to destroy the cell membrane of frond, utilize protein in composite protease hydrolysis sea-tangle and marine alga starch etc.; Adopting aperture is that organic tubular membrane of 0.1-0.2um is carried out filtration treatment again, and this tubular membrane material liquid flowing channel is wide, allows the material of high suspended matter content to enter, and pretreatment is simple, and its solid content is automatically separate with clear liquid, and separating and filtering efficiency is high, has realized serialization production.The preparation method technique of kelp dietary fiber of the present invention is reasonable, easy and simple to handle, workable, easily realizes suitability for industrialized production.
The specific embodiment
below in conjunction with embodiment, the present invention is described in further details.
embodiment 1
A preparation method for kelp dietary fiber, process following process steps: choosing dry sea-tangle 100kg is raw material, soaks and desalts with the clear water of 10 times of dry sea-tangle quality, after frond is fully softening, adopt flowing water to clean, draining, weigh, measure its protein content, with tissue mashing machine's processing of pulling an oar; Add the water of its quality 6 to dilute kelp paste, regulate pH value 4.5, add the two enzymes of cellulase and compound protease, its cellulase and compound protease can be commercial commercial enzyme, the enzyme activity 15000u/g of cellulase; Compound protease adopts Protamex compound protease, enzyme activity 50000u/g; Control temperature 50 C, enzymolysis 3h, described cellulase, the enzyme dosage of compound protease are 4% of sea-tangle protein content; After enzymolysis, heat 95 ℃, the enzyme 15min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 4%, regulate pH value 6.5, then adopt tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.2 μ m, and operating temperature is controlled at 40 ℃, enter film pressure and be controlled at 2.9bar, go out film pressure and be controlled at 1.0 bar; Filtered solution flow is at 45-95L/h, and after continuous operation 5h, filtered solution soluble solid content is down to 0%, and stop cleaning is collected trapped substance, dry, obtains kelp dietary fiber 26.7kg.
embodiment 2
A preparation method for kelp dietary fiber, through following process steps: choosing dry sea-tangle 200kg is raw material, with 12 times of clear water of dry sea-tangle quality, soak desalt, after frond softens, employing flowing water is cleaned, draining, weighs, measures its protein content, with tissue mashing machine's processing of pulling an oar; Add the water of 5 times of its quality to dilute kelp paste, regulate pH value 4.0, add the two enzymes of cellulase and compound protease, its cellulase is commercial commercial enzyme, the enzyme activity 15000u/g of cellulase; Compound protease is prepared voluntarily, in the compound protein hydrolase of preparation, contains the plurality of enzymes such as papain, amylase, enzyme activity 52000u/g; Control 55 ℃ of temperature, enzymolysis 2h, described cellulase, the enzyme dosage of compound protease are 5% of sea-tangle protein content: after enzymolysis, heat 100 ℃, the enzyme 10min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 5%, regulate pH value 6.0, then adopt tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.1 μ m, and operating temperature is controlled at 40 ℃, enter film pressure and be controlled at 2.5bar, go out film pressure and be controlled at 1.0bar; Filtered solution flow is at 40-90L/h, can continuous operation 4.5h after, filtered solution flux declines 70%, stop cleaning is collected trapped substance, dry, obtains kelp dietary fiber 51.8kg.
embodiment 3
A preparation method for kelp dietary fiber, process following process steps: choosing dry sea-tangle 100kg is raw material, soaks and desalts with the clear water of 8 times, dry sea-tangle, after frond is fully softening, adopt flowing water to clean, draining, weigh, measure its protein content, with tissue mashing machine's processing of pulling an oar; Add the water of 10 times of its quality to dilute kelp paste, regulate pH value 5.0, add the two enzymes of cellulase and compound protease, its cellulase and compound protease can be commercial commercial enzyme, the enzyme activity 15000u/g of cellulase; Compound protease adopts Protamex compound protease, enzyme activity 55000u/g; Control 40 ℃ of temperature, enzymolysis 4h, described cellulase, the enzyme dosage of compound protease are 2% of sea-tangle protein content: after enzymolysis, heat 90 ℃, the enzyme 20min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 3%, regulate pH value 7.0, then adopt tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.2 μ m, and operating temperature is controlled at 30 ℃, enter film pressure and be controlled at 3.0bar, go out film pressure and be controlled at 0.9bar; Filtered solution flow is at 30-70L/h, can continuous operation 3h after, filtered solution flux declines 65%, stop cleaning is collected trapped substance, dry, obtains kelp dietary fiber 27.1kg.
the physiological function test of the kelp dietary fiber that the inventive method is produced.
Choose and have 40 of the voluntary tested patients of constipation history, age 30-70 year, (45 years old mean age), once, 25% people's 3 days stool of people of 75% defecates weekly once, constipation history 0.5-5.Take the kelp dietary fiber of system of the present invention, every day 3 times, each 2g, links and takes 30 days, patient's constipation and alleviation situation in detail, and do detailed record.
Its result is as follows: there is the voluntary tested patient of constipation history to take through 40, and short 1-2 days, long about 5 days, start to occur normally or significantly alleviate.Wherein, in 40 patients, defecation 1 time every day 34 (accounting for 85%), 4 (the accounting for 10%) that every 2 days defecations are 1 time, 2 (the accounting for 5%) that every 3 days defecations are 1 time.Natural rate of interest is 85%, and normal rate is 10%, and efficient is 100%.Illustrate that the kelp dietary fiber that the inventive method is produced has good effect of relaxing bowel.
Claims (1)
1. a preparation method for kelp dietary fiber, is characterized in that: process following process steps:
Choosing dry sea-tangle is raw material, with clear water, soak desalt, softening after, adopt flowing water to clean, draining, weighs, measures its protein content, with tissue mashing machine's processing of pulling an oar; Add its quality 5-10 water doubly to dilute kelp paste, regulate pH value 4.0~5.0, add the two enzymes of cellulase and compound protease, control temperature 40-55 ℃, enzymolysis 2-4h, described cellulase, the enzyme dosage of compound protease are the 2-5% of sea-tangle protein content: after enzymolysis, heating 90-100 ℃, enzyme 10~the 20min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 3-5%, regulate pH value 6.0-7.0, adopt again tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.1~0.2 μ m, operating temperature is controlled at 30~45 ℃, enters film pressure and is controlled at 2.5~3.0bar, goes out film pressure and is controlled at 0.8~1.0 bar; Filtered solution soluble solid content be down to after 0% or flux decline 65-70% after stop cleaning, collect trapped substance, obtain kelp dietary fiber.
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CN201310649386.4A CN103719879B (en) | 2013-12-06 | 2013-12-06 | A kind of preparation method of kelp dietary fiber |
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CN201310649386.4A CN103719879B (en) | 2013-12-06 | 2013-12-06 | A kind of preparation method of kelp dietary fiber |
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CN103719879B CN103719879B (en) | 2016-05-18 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105192766A (en) * | 2015-11-16 | 2015-12-30 | 天津春发生物科技集团有限公司 | Method for preparing kelp powder by microwave compound enzyme method |
CN105533370A (en) * | 2015-12-30 | 2016-05-04 | 山东好当家海洋发展股份有限公司 | Preparation method of kelp-probiotics beverage |
CN106092815A (en) * | 2016-06-27 | 2016-11-09 | 江西出入境检验检疫局检验检疫综合技术中心 | The assay method of total dietary fiber in a kind of food |
CN108030103A (en) * | 2017-11-28 | 2018-05-15 | 齐鲁工业大学 | A kind of hypoglycemic preparation method with seaweed diet fiber containing probiotics |
Citations (1)
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CN101756295A (en) * | 2008-11-21 | 2010-06-30 | 威海健方医药研究所 | Method for preparing kelp dietary fiber by ultrasonic enzymolysis |
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2013
- 2013-12-06 CN CN201310649386.4A patent/CN103719879B/en active Active
Patent Citations (1)
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CN101756295A (en) * | 2008-11-21 | 2010-06-30 | 威海健方医药研究所 | Method for preparing kelp dietary fiber by ultrasonic enzymolysis |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105192766A (en) * | 2015-11-16 | 2015-12-30 | 天津春发生物科技集团有限公司 | Method for preparing kelp powder by microwave compound enzyme method |
CN105533370A (en) * | 2015-12-30 | 2016-05-04 | 山东好当家海洋发展股份有限公司 | Preparation method of kelp-probiotics beverage |
CN106092815A (en) * | 2016-06-27 | 2016-11-09 | 江西出入境检验检疫局检验检疫综合技术中心 | The assay method of total dietary fiber in a kind of food |
CN108030103A (en) * | 2017-11-28 | 2018-05-15 | 齐鲁工业大学 | A kind of hypoglycemic preparation method with seaweed diet fiber containing probiotics |
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