CN103719879A - Method for manufacturing laminaria japonica aresch dietary fiber - Google Patents

Method for manufacturing laminaria japonica aresch dietary fiber Download PDF

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Publication number
CN103719879A
CN103719879A CN201310649386.4A CN201310649386A CN103719879A CN 103719879 A CN103719879 A CN 103719879A CN 201310649386 A CN201310649386 A CN 201310649386A CN 103719879 A CN103719879 A CN 103719879A
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China
Prior art keywords
dietary fiber
laminaria japonica
tangle
sea
water
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Granted
Application number
CN201310649386.4A
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Chinese (zh)
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CN103719879B (en
Inventor
张绵松
刘昌衡
胡炜
乔若瑾
齐君
孙永军
史亚萍
夏雪奎
袁文鹏
张永刚
贾爱荣
鞠文明
唐晓波
毕春雨
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Biology Institute of Shandong Academy of Sciences
Shandong Homey Aquatic Development Co Ltd
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Biology Institute of Shandong Academy of Sciences
Shandong Homey Aquatic Development Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L17/00Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
    • A23L17/60Edible seaweed
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Edible Seaweed (AREA)

Abstract

The invention relates to a method for manufacturing laminaria japonica aresch dietary fiber. The method comprises the following steps: soaking dry laminaria japonica aresch taken as a raw material with water for desalination and softening, cleaning, draining water, and beating; adding water into the laminaria japonica aresch slurry for dilution, adding cellulase and compound protease double enzyme, controlling the enzymolysis condition for enzymolysis, and carrying out enzyme deactivation to obtain a laminaria japonica aresch enzymatic hydrolysate; adjusting the concentration and the pH value of the laminaria japonica aresch enzymatic hydrolysate, filtering the laminaria japonica aresch enzymatic hydrolysate with a tubular membrane, drying, and collecting a retention object to obtain the laminaria japonica aresch dietary fiber. The method is reasonable in process, simple and convenient to operate, high in operability, and easy to realize industrialized production; the laminaria japonica aresch dietary fiber manufactured according to the method is high in bowel relaxing efficacy.

Description

A kind of preparation method of kelp dietary fiber
Technical field
The present invention relates to produce cellulosic other processes, especially a kind of preparation method of kelp dietary fiber.
Background technology
Dietary fiber is current a kind of functional food ingredient of generally acknowledging in the world, has unique trophic function in human body, and it can promote gastrointestinal peristalsis, increases the secretion of digestive juice, reduces the absorption of harmful substance; Can also integrate Dan Gu Tun, reduce the absorption of Dan Gu Tun, Gao Dan Gu Tun mass formed by blood stasis and artery sclerosis are played to prevention effect, therefore be called as the 3rd nutrient except water, protein, carbohydrate, lipid, vitamin, the large nutrient of mineral matter six, and be described as in 21 century food industry of greatest concernly, there are a kind of food additives of special health care and food modifying function.Dietary fiber is a kind of mixture of complexity, raw material sources difference, and also there is difference in the physicochemical properties that show, and the physiological activity being risen also has power.
At present, the research overwhelming majority of China's dietary fiber rests on terrestrial plant aspect, and less to marine field the Study on Resources.The nutritive value of sea-tangle is very high, is rich in algin, dietary fiber and mineral matter etc., is the good source that produces dietary fiber.
Summary of the invention
The object of this invention is to provide that a kind of technique is reasonable, easy and simple to handle, the preparation method of the kelp dietary fiber of advanced technology.
The technical solution adopted for the present invention to solve the technical problems is: a kind of preparation method of kelp dietary fiber, is characterized in that: process following process steps:
Choosing dry sea-tangle is raw material, with clear water, soak desalt, softening after, adopt flowing water to clean, draining, weighs, measures its protein content, with tissue mashing machine's processing of pulling an oar; Add its quality 5-10 water doubly to dilute kelp paste, regulate pH value 4.0~5.0, add the two enzymes of cellulase and compound protease, control temperature 40-55 ℃, enzymolysis 2-4h, described cellulase, the enzyme dosage of compound protease are the 2-5% of sea-tangle protein content: after enzymolysis, heating 90-100 ℃, enzyme 10~the 20min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 3-5%, regulate pH value 6.0-7.0, adopt again tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.1~0.2 μ m, operating temperature is controlled at 30~45 ℃, enters film pressure and is controlled at 2.5~3.0bar, goes out film pressure and is controlled at 0.8~1.0 bar; Filtered solution soluble solid content be down to after 0% or flux decline 65-70% after stop cleaning, collect trapped substance, dry, obtain kelp dietary fiber.
The present invention be dry sea-tangle raw material is soaked in water desalt, softening after, clean draining, making beating; Kelp paste adds water dilution, adds the two enzymes of cellulase and compound protease, and controlled enzymatic hydrolysis condition is carried out enzymolysis processing, and the enzyme that goes out obtains sea-tangle enzymolysis liquid; After sea-tangle enzymolysis liquid is adjusted to material concentration, pH value, adopt tubular membrane to filter, collect trapped substance, obtain kelp dietary fiber.This trapped substance is the unhydrolyzable part after water extraction and enzymolysis and extraction, is rich in crude fibre, has unique local flavor and the effect relaxing bowel.In the enzymolysis process of sea-tangle slurries of the present invention, be to utilize cellulase effectively to destroy the cell membrane of frond, utilize protein in composite protease hydrolysis sea-tangle and marine alga starch etc.; Adopting aperture is that organic tubular membrane of 0.1-0.2um is carried out filtration treatment again, and this tubular membrane material liquid flowing channel is wide, allows the material of high suspended matter content to enter, and pretreatment is simple, and its solid content is automatically separate with clear liquid, and separating and filtering efficiency is high, has realized serialization production.The preparation method technique of kelp dietary fiber of the present invention is reasonable, easy and simple to handle, workable, easily realizes suitability for industrialized production.
The specific embodiment
below in conjunction with embodiment, the present invention is described in further details.
embodiment 1
A preparation method for kelp dietary fiber, process following process steps: choosing dry sea-tangle 100kg is raw material, soaks and desalts with the clear water of 10 times of dry sea-tangle quality, after frond is fully softening, adopt flowing water to clean, draining, weigh, measure its protein content, with tissue mashing machine's processing of pulling an oar; Add the water of its quality 6 to dilute kelp paste, regulate pH value 4.5, add the two enzymes of cellulase and compound protease, its cellulase and compound protease can be commercial commercial enzyme, the enzyme activity 15000u/g of cellulase; Compound protease adopts Protamex compound protease, enzyme activity 50000u/g; Control temperature 50 C, enzymolysis 3h, described cellulase, the enzyme dosage of compound protease are 4% of sea-tangle protein content; After enzymolysis, heat 95 ℃, the enzyme 15min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 4%, regulate pH value 6.5, then adopt tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.2 μ m, and operating temperature is controlled at 40 ℃, enter film pressure and be controlled at 2.9bar, go out film pressure and be controlled at 1.0 bar; Filtered solution flow is at 45-95L/h, and after continuous operation 5h, filtered solution soluble solid content is down to 0%, and stop cleaning is collected trapped substance, dry, obtains kelp dietary fiber 26.7kg.
embodiment 2
A preparation method for kelp dietary fiber, through following process steps: choosing dry sea-tangle 200kg is raw material, with 12 times of clear water of dry sea-tangle quality, soak desalt, after frond softens, employing flowing water is cleaned, draining, weighs, measures its protein content, with tissue mashing machine's processing of pulling an oar; Add the water of 5 times of its quality to dilute kelp paste, regulate pH value 4.0, add the two enzymes of cellulase and compound protease, its cellulase is commercial commercial enzyme, the enzyme activity 15000u/g of cellulase; Compound protease is prepared voluntarily, in the compound protein hydrolase of preparation, contains the plurality of enzymes such as papain, amylase, enzyme activity 52000u/g; Control 55 ℃ of temperature, enzymolysis 2h, described cellulase, the enzyme dosage of compound protease are 5% of sea-tangle protein content: after enzymolysis, heat 100 ℃, the enzyme 10min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 5%, regulate pH value 6.0, then adopt tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.1 μ m, and operating temperature is controlled at 40 ℃, enter film pressure and be controlled at 2.5bar, go out film pressure and be controlled at 1.0bar; Filtered solution flow is at 40-90L/h, can continuous operation 4.5h after, filtered solution flux declines 70%, stop cleaning is collected trapped substance, dry, obtains kelp dietary fiber 51.8kg.
embodiment 3
A preparation method for kelp dietary fiber, process following process steps: choosing dry sea-tangle 100kg is raw material, soaks and desalts with the clear water of 8 times, dry sea-tangle, after frond is fully softening, adopt flowing water to clean, draining, weigh, measure its protein content, with tissue mashing machine's processing of pulling an oar; Add the water of 10 times of its quality to dilute kelp paste, regulate pH value 5.0, add the two enzymes of cellulase and compound protease, its cellulase and compound protease can be commercial commercial enzyme, the enzyme activity 15000u/g of cellulase; Compound protease adopts Protamex compound protease, enzyme activity 55000u/g; Control 40 ℃ of temperature, enzymolysis 4h, described cellulase, the enzyme dosage of compound protease are 2% of sea-tangle protein content: after enzymolysis, heat 90 ℃, the enzyme 20min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 3%, regulate pH value 7.0, then adopt tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.2 μ m, and operating temperature is controlled at 30 ℃, enter film pressure and be controlled at 3.0bar, go out film pressure and be controlled at 0.9bar; Filtered solution flow is at 30-70L/h, can continuous operation 3h after, filtered solution flux declines 65%, stop cleaning is collected trapped substance, dry, obtains kelp dietary fiber 27.1kg.
the physiological function test of the kelp dietary fiber that the inventive method is produced.
Choose and have 40 of the voluntary tested patients of constipation history, age 30-70 year, (45 years old mean age), once, 25% people's 3 days stool of people of 75% defecates weekly once, constipation history 0.5-5.Take the kelp dietary fiber of system of the present invention, every day 3 times, each 2g, links and takes 30 days, patient's constipation and alleviation situation in detail, and do detailed record.
Its result is as follows: there is the voluntary tested patient of constipation history to take through 40, and short 1-2 days, long about 5 days, start to occur normally or significantly alleviate.Wherein, in 40 patients, defecation 1 time every day 34 (accounting for 85%), 4 (the accounting for 10%) that every 2 days defecations are 1 time, 2 (the accounting for 5%) that every 3 days defecations are 1 time.Natural rate of interest is 85%, and normal rate is 10%, and efficient is 100%.Illustrate that the kelp dietary fiber that the inventive method is produced has good effect of relaxing bowel.

Claims (1)

1. a preparation method for kelp dietary fiber, is characterized in that: process following process steps:
Choosing dry sea-tangle is raw material, with clear water, soak desalt, softening after, adopt flowing water to clean, draining, weighs, measures its protein content, with tissue mashing machine's processing of pulling an oar; Add its quality 5-10 water doubly to dilute kelp paste, regulate pH value 4.0~5.0, add the two enzymes of cellulase and compound protease, control temperature 40-55 ℃, enzymolysis 2-4h, described cellulase, the enzyme dosage of compound protease are the 2-5% of sea-tangle protein content: after enzymolysis, heating 90-100 ℃, enzyme 10~the 20min that goes out, obtains sea-tangle enzymolysis liquid; Sea-tangle enzymolysis liquid is added to water and adjust material concentration 3-5%, regulate pH value 6.0-7.0, adopt again tubular membrane to filter, wherein, the work aperture of described tubular membrane is controlled at 0.1~0.2 μ m, operating temperature is controlled at 30~45 ℃, enters film pressure and is controlled at 2.5~3.0bar, goes out film pressure and is controlled at 0.8~1.0 bar; Filtered solution soluble solid content be down to after 0% or flux decline 65-70% after stop cleaning, collect trapped substance, obtain kelp dietary fiber.
CN201310649386.4A 2013-12-06 2013-12-06 A kind of preparation method of kelp dietary fiber Active CN103719879B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105192766A (en) * 2015-11-16 2015-12-30 天津春发生物科技集团有限公司 Method for preparing kelp powder by microwave compound enzyme method
CN105533370A (en) * 2015-12-30 2016-05-04 山东好当家海洋发展股份有限公司 Preparation method of kelp-probiotics beverage
CN106092815A (en) * 2016-06-27 2016-11-09 江西出入境检验检疫局检验检疫综合技术中心 The assay method of total dietary fiber in a kind of food
CN108030103A (en) * 2017-11-28 2018-05-15 齐鲁工业大学 A kind of hypoglycemic preparation method with seaweed diet fiber containing probiotics

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101756295A (en) * 2008-11-21 2010-06-30 威海健方医药研究所 Method for preparing kelp dietary fiber by ultrasonic enzymolysis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101756295A (en) * 2008-11-21 2010-06-30 威海健方医药研究所 Method for preparing kelp dietary fiber by ultrasonic enzymolysis

Non-Patent Citations (3)

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Title
付全意等: "膳食纤维提取方法的研究进展", 《食品科技》 *
朱蓓薇等: "《水产品加工工艺学》", 31 January 2011 *
潘文洁等: "海带膳食纤维不同提取方法的研究", 《安徽工程科技学院学报》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105192766A (en) * 2015-11-16 2015-12-30 天津春发生物科技集团有限公司 Method for preparing kelp powder by microwave compound enzyme method
CN105533370A (en) * 2015-12-30 2016-05-04 山东好当家海洋发展股份有限公司 Preparation method of kelp-probiotics beverage
CN106092815A (en) * 2016-06-27 2016-11-09 江西出入境检验检疫局检验检疫综合技术中心 The assay method of total dietary fiber in a kind of food
CN108030103A (en) * 2017-11-28 2018-05-15 齐鲁工业大学 A kind of hypoglycemic preparation method with seaweed diet fiber containing probiotics

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