CN103718958B - Sponge fluid nutrient medium and the application thereof of the breeding of cold blue tissue culture sprout quick speed - Google Patents
Sponge fluid nutrient medium and the application thereof of the breeding of cold blue tissue culture sprout quick speed Download PDFInfo
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- CN103718958B CN103718958B CN201310696835.0A CN201310696835A CN103718958B CN 103718958 B CN103718958 B CN 103718958B CN 201310696835 A CN201310696835 A CN 201310696835A CN 103718958 B CN103718958 B CN 103718958B
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- Prior art keywords
- sponge
- fluid nutrient
- nutrient medium
- medium
- cold blue
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 45
- 239000012530 fluid Substances 0.000 title claims abstract description 38
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 27
- 229920001817 Agar Polymers 0.000 claims abstract description 6
- 239000008272 agar Substances 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 4
- 229940079593 drug Drugs 0.000 claims abstract description 4
- 239000000203 mixture Substances 0.000 claims abstract description 4
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 3
- 239000002609 medium Substances 0.000 claims description 40
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000012856 packing Methods 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 238000005213 imbibition Methods 0.000 description 2
- 150000004053 quinones Chemical class 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Hydroponics (AREA)
Abstract
The invention discloses the sponge fluid nutrient medium of cold blue tissue culture sprout quick speed breeding, that on sponge, evenly to have 15 bores be 0.8cm, the degree of depth is the V-shape hole of 0.5cm size, sponge block is lain in to blake bottle bottom, one of perforate faces up, directly prepare medicines with running water and through constant volume mix up pH value fluid nutrient medium, appropriate point installs in the blake bottle that is placed with sponge, nutrient solution has just been invaded and finish sponge block; The component of fluid nutrient medium consists of B5 medium+TDZ plant growth regulator 0.8mg/L+ methyl α-naphthyl acetate 0.2-0.8mg/L. Beneficial effect of the present invention is: sponge fluid nutrient medium provided by the invention, has the following advantages: 1, cold blue group training material can fully absorb nutrition, improves quality and output, and the rate of increase can reach 5-6 doubly; 2, sponge can reuse, and fluid nutrient medium packing preparation is more convenient, and the apparatus such as bottle easily clean up; 3, save the expense of buying agar powder, reduce production costs.
Description
Technical field
The present invention relates to the fast numerous field of plant tissue culture seedling, be specifically related to sponge fluid nutrient medium and the application thereof of cold blue tissue culture sprout quick speed breeding.
Background technology
At present, the cold blue group training operator schemes that adopt simple Liquid culture medium+solid medium more, but this kind of training method efficiency is lower, and the rate of increase is low, and the quality of cold blue group training seedling plant is not high, and wherein used medium cost is higher.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, provides one can improve cold blue group training rate of increase sponge fluid nutrient medium.
To achieve these goals, technical scheme provided by the invention is: the sponge fluid nutrient medium of cold blue tissue culture sprout quick speed breeding, to select columniform high temperature resistant filtration sponge, diameter and ventilating cover thread bottle bottom are in the same size, sponge thickness is 1cm, on sponge, evenly having 15 bores is 0.8cm, the degree of depth is the V-shape hole of 0.5cm size, sponge block is lain in to blake bottle bottom, one of perforate faces up, directly prepare medicines with running water and through constant volume mix up pH value fluid nutrient medium, appropriate point installs in the blake bottle that is placed with sponge, nutrient solution is just invaded and finish sponge block, the component of described fluid nutrient medium consists of B5 medium+TDZ plant growth regulator 0.8mg/L+ methyl α-naphthyl acetate 0.2-0.8mg/L.
Second object of the present invention has been to provide the application of above-mentioned sponge fluid nutrient medium in the breeding of cold blue tissue culture sprout quick speed.
Further, above-mentioned application, comprises the following steps:
1) by the above-mentioned point sponge fluid nutrient medium installing through autoclaving, sterilising temp is 121-123 DEG C, sterilizing pressure is 0.11-0.13MP, sterilization time is 22-25 minute, obtains culture medium after sterilizing;
2) inoculation of culture materials: under germ-free condition, cold blue culture materials is cut into simple bud and insert in the V-shape hole of planting the sponge fluid nutrient medium after sterilizing respectively, carry out fast breeding cultivation, incubation time is 30-35 days, and the rate of increase is 5-6 times;
3) switching of fertile material: by step 2) in material after propagation hold under the arm out to cut to inoculate and in fresh fluid nutrient medium, proceed propagation and cultivate; After this in fresh fluid nutrient medium, breed cultivation when cultivating to 30-35 days time, again material is cut to change to;
4) culture of rootage: the cold blue fertile material after the propagation that step 3) is obtained is cultivated is transferred to after some, cut into individual plant and be transferred to and in the solid medium that is added with agar powder, carry out culture of rootage, after within 2～3 months, cultivating hardening, obtain cold blue group training seedling and transplant strain.
Beneficial effect of the present invention is: sponge fluid nutrient medium provided by the invention, has the following advantages:
1, cold blue group training material can fully absorb nutrition, improves quality and output, and the rate of increase can reach 5-6 doubly;
2, sponge can reuse, and fluid nutrient medium packing preparation is more convenient, and the apparatus such as bottle easily clean up;
3, save the expense of buying agar powder, reduce production costs.
Detailed description of the invention
Embodiment 1:
(1) making of sponge fluid nutrient medium:
Select columniform high temperature resistant filtration sponge, diameter and ventilating cover thread bottle bottom are in the same size, and sponge thickness is 1cm, on sponge, evenly having 15 bores is 0.8cm, the degree of depth is the V-shape hole of 0.5cm size, and sponge block is lain in to blake bottle bottom, and one of perforate faces up. Because not adding agar powder, can directly prepare medicines with running water and through constant volume mix up pH value fluid nutrient medium, last appropriate point installs in the blake bottle that is placed with sponge, nutrient solution has just been invaded and finish sponge block;
(2) sterilizing of culture medium:
Because the sponge of selecting is exotic material, main component is polyurethane, have anticorrosive, have no side effect, high temperature resistant (can reach 200 DEG C), intensity high (resilience is good), water imbibition be extremely strong, the advantages such as recoverable, through after autoclaving repeatedly, modification is still not reusable; Divide the culture medium installing through autoclaving (sterilising temp is 121-123 degree, and pressure is 0.11-0.13MP, and the time is 22-25 minute), finally the good fluid nutrient medium of sterilizing is put into culture medium storeroom for subsequent use;
(3) inoculation of culture materials:
Culture materials being cut into simple bud on aseptic working platform inserts and plants in the V-shape hole of sponge fluid nutrient medium respectively, because sponge has good resilience and water imbibition, the culture materials being inserted in V-shape hole is had to certain fixation and moisture-keeping functions, be beneficial to the growth of culture and the absorption of nutrient;
(4) propagation of culture materials is cultivated:
Cultivate the material on sponge fluid nutrient medium, due to molecule in fluid nutrient medium or ion motion very fast, its base portion can fully touch nutrient solution and can absorb preferably nutrient, the quinones harmful substance of simultaneously also self otch being secreted is out diluted in fluid nutrient medium, reduce the generation of brownization, thereby reach the effect of fast breeding, generally cultivate the rate of increase after 30-35 days and can reach 5-6 doubly;
(5) switching of fertile material:
When cultivating after 30-35 days in fertile material liquid medium within, culture materials is proliferate constantly, has absorbed most nutrition and moisture, has also accumulated the harmful components such as certain quinones in fluid nutrient medium simultaneously; Now the material after propagation should be held under the arm out to cut to inoculate and in fresh fluid nutrient medium, proceed propagation and cultivate, after this in the time cultivating 30-35 days, constantly material is cut to change in fresh fluid nutrient medium and breed cultivation, can make to produce the material that reaches some and scale;
(6) culture of rootage:
Cold blue fertile material is transferred to be produced after required quantity, is cut into individual plant and is transferred in the solid medium that is added with agar powder and carries out culture of rootage, after 2～3 months cultivate hardening, can transplant by bottle outlet.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement. Within the spirit and principles in the present invention all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (1)
1. the application of sponge fluid nutrient medium in the breeding of cold blue tissue culture sprout quick speed, it is characterized in that, sponge fluid nutrient medium is to select columniform high temperature resistant filtration sponge, diameter and ventilating cover thread bottle bottom are in the same size, sponge thickness is 1cm, on sponge, evenly having 15 bores is 0.8cm, the degree of depth is the V-shape hole of 0.5cm size, sponge block is lain in to blake bottle bottom, one of perforate faces up, directly prepare medicines with running water and through constant volume mix up pH value fluid nutrient medium, appropriate point installs in the blake bottle that is placed with sponge, make nutrient solution just soak sponge block, the component of described fluid nutrient medium consists of B5 medium+TDZ plant growth regulator 0.8mg/L+ methyl α-naphthyl acetate 0.2-0.8mg/L,
The application of sponge fluid nutrient medium in the breeding of cold blue tissue culture sprout quick speed comprises the following steps:
1) the sponge fluid nutrient medium that will point install is through autoclaving, and sterilising temp is 121-123 DEG C, and sterilizing pressure is 0.11-0.13MP, and sterilization time is 22-25 minute, obtains culture medium after sterilizing;
2) inoculation of culture materials: under germ-free condition, cold blue culture materials is cut into simple bud and insert in the V-shape hole of planting the sponge fluid nutrient medium after sterilizing respectively, carry out fast breeding cultivation, incubation time is 30-35 days, and the rate of increase is 5-6 times;
3) switching of fertile material: by step 2) in material after propagation hold under the arm out to cut to inoculate and in fresh fluid nutrient medium, proceed propagation and cultivate; After this in fresh fluid nutrient medium, breed cultivation when cultivating to 30-35 days time, again material is cut to change to;
4) culture of rootage: the cold blue fertile material after the propagation that step 3) is obtained is cultivated is transferred to after some, cut into individual plant and be transferred to and in the solid medium that is added with agar powder, carry out culture of rootage, after within 2～3 months, cultivating hardening, obtain cold blue group training seedling and transplant strain.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310696835.0A CN103718958B (en) | 2013-12-18 | 2013-12-18 | Sponge fluid nutrient medium and the application thereof of the breeding of cold blue tissue culture sprout quick speed |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310696835.0A CN103718958B (en) | 2013-12-18 | 2013-12-18 | Sponge fluid nutrient medium and the application thereof of the breeding of cold blue tissue culture sprout quick speed |
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| Publication Number | Publication Date |
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| CN103718958A CN103718958A (en) | 2014-04-16 |
| CN103718958B true CN103718958B (en) | 2016-05-25 |
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| CN103734008B (en) * | 2013-12-26 | 2016-04-13 | 广西森仙源生物科技有限公司 | A kind of butterfly orchid tissue cultivating Fast-propagation liquid nutrient medium and corresponding cultural method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695283A (en) * | 2009-11-10 | 2010-04-21 | 北京师范大学 | Culture medium for tissue culture of pedicel buds of oncidium hybridum and tissue cultured seedling propagating method |
| CN103004608A (en) * | 2013-01-08 | 2013-04-03 | 四川农业大学 | Culture medium for culturing hoya tissue and culture method |
| CN103314852A (en) * | 2013-06-28 | 2013-09-25 | 上海市农业科学院 | Method for efficiently propagating dendrobium by using roots and culture medium of dendrobium |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695283A (en) * | 2009-11-10 | 2010-04-21 | 北京师范大学 | Culture medium for tissue culture of pedicel buds of oncidium hybridum and tissue cultured seedling propagating method |
| CN103004608A (en) * | 2013-01-08 | 2013-04-03 | 四川农业大学 | Culture medium for culturing hoya tissue and culture method |
| CN103314852A (en) * | 2013-06-28 | 2013-09-25 | 上海市农业科学院 | Method for efficiently propagating dendrobium by using roots and culture medium of dendrobium |
Non-Patent Citations (3)
| Title |
|---|
| 中国兰的组织培养;徐程;《植物生理学通讯》;20020430;第38卷(第2期);第171页摘要，第1.1节，第172页第2.2节 * |
| 寒兰的快速繁殖技术;朱国兵;《热带亚热带植物学报》;20061231;第14卷(第2期);第151页摘要 * |
| 载体式培养基的制备与应用;陈其皎等;《食用菌》;20021231(第5期);第1.2节和第2.1-2.3节 * |
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Effective date of registration: 20170925 Address after: Calculation of business R & D center eleven layer research room 530029 Nanning city the Guangxi Zhuang Autonomous Region Qingxiu District Bauhinia Road No. 1 Nanning international internet dedicated channel and cloud Patentee after: GUANGXI YIMU AGRICULTURAL TECHNOLOGY Co.,Ltd. Address before: 545002, the Guangxi Zhuang Autonomous Region, Liuzhou North Long Pond flower demonstration Office Patentee before: LIUZHOU TIANZI HORTICULTURE Co.,Ltd. |
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