CN103706411A - Device for rapid dehydration and of fixing biological molecule detection sample and method - Google Patents
Device for rapid dehydration and of fixing biological molecule detection sample and method Download PDFInfo
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- CN103706411A CN103706411A CN201310517752.0A CN201310517752A CN103706411A CN 103706411 A CN103706411 A CN 103706411A CN 201310517752 A CN201310517752 A CN 201310517752A CN 103706411 A CN103706411 A CN 103706411A
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Abstract
The invention discloses a device for rapid dehydration and fixing of a biological molecule detection sample and a use method thereof. Main parts of the device are as follows: a cylindrical bottle body with a diameter of 25-60 mm and a height of 35-75 mm and a round wire mouth bottle cap, a sample storage container is arranged in the bottle body or the bottle cap, a filter paper, cavernous body and biological molecule protectant is put in the sample storage container, an additive bag containing a desiccant, porous sponge foam and a deoxidizer is placed at the bottom of the bottle body, an isolation device is arranged between the additive bag and the sample storage container for preventing the additives from polluting the sample, and the isolation device is provided with multiple air holes convenient for the additive bag to play effects of drying, deoxidization, and the like. The device can fast dry the biological sample and preserve the biological sample for a long term, and has very broad application prospects.
Description
Technical field:
The present invention relates to a kind of device for biomolecule Sample storage and using method thereof.
Background technology:
The main component of blood of human body is blood plasma and haemocyte.The inorganic salts that contain in blood plasma, products of cellular metabolism, hormone, enzyme and antibody etc. have important physiological function, have carried the health and fitness information of human body.Haemocyte can be divided into red blood cell, leucocyte, blood platelet, and red blood cell has ABO type antigen, is some hemopathic diagnosis, curative effect judgement and one of estimation and the indexs such as anaemia; Leucocyte is the colourless sphaerocyst that has core that volume ratio red blood cell is slightly large, and leukocytic quantity can reflect the health status of human body; Blood platelet has specific morphosis and biochemical composition, in the physiology such as hemostasis, wound healing, inflammatory reaction, thrombosis and organ-graft refection and pathologic process, plays an important role.Therefore, the physiological change of organism and pathological change often cause the change of blood constituent, so the detection of blood constituent has important clinical meaning.
Urine be the mankind and vertebrate in metabolic processes, due to the needs of body, the liquid discharges excreting via urinary system and urinary tract.The health that in urine, entrained information can reflect human body whether, is also in the activities such as important sports events, the index whether sportsman's illegal drug is used.Urine examination is a very important clinically trial inspection, urine examination process is had relatively high expectations to the preservation of the sample of collecting, because place red blood cell and the leucocyte in urine after several hours after urine sample takes out, be just damaged, glucose is decomposed etc. by bacterium, affects the accuracy of check result.The requirement that urine examination sample in important sports events need to be preserved is preserved and is reached the several years, as the detection in later stage.Therefore, need a kind of store method fast and effectively, to guarantee the accuracy of testing result.
In addition, other various body fluid, as saliva, seminal fluid, and the cell in artificial bio-membrane's sample, nutrient solution, extract etc., before detection, all need through storing and processing, therefore, it is particularly important that the preservation of sample seems.
With the store method of blood testing sample in prior art, detecting is at present by adding the method for anticorrisive agent, glass seasoning and freeze-drying etc. to preserve with blood testing samples such as serum.The shortcoming of this store method is apparent: the method term of validity that 1, adds anticorrisive agent to preserve only has two weeks, easily grows mould meeting contaminated samples, the active ingredient in sample is changed, thereby affect the accuracy of result of use and testing result.2, freeze-drying is in freezing and dry process, and the low temperature of moment makes the volume of sample and surface pressure occur to change rapidly, and the molecule in sample is caused to damage.3, in conventional detection, for doing for the sample of different test items respectively, preserve, work loaded down with trivial details and complicated.Therefore seeking a kind of sample product for the treatment of that can be quick, stable under normal temperature condition preserves respectively and seems particularly important.
Summary of the invention:
Fast dewatering fixing biological molecules detects a device for sample, is a kind of rapid draing design box, comprising: cylindrical bottle, and diameter is 25～60mm, height is 35～75mm; Circular wire mouth bottle cap; In bottle or bottle cap, be provided with sample reservoir vessel, be placed with filter paper, the cavernous body with certain water absorbing capacity, the biomolecule protective agent being formed by trehalose, maltose, maltodextrin, HES etc. and a small amount of glycerine; Bottle bottom is placed with auxiliary agent bag; Between auxiliary agent bag and sample reservoir vessel, there is spacer assembly; On spacer assembly, a plurality of air-vents can be set, air-vent is circle, ellipse or rectangle, and auxiliary agent bag comprises: one or more drier, porous spongy foam and deoxidier.Deoxidier can be inner at porous foam, can be also independently.
The material of quick-drying design box of the present invention is the material with good biocompatibility.In preservation process, can not produce sample is had to the gas of pollution and powder etc.Quick-drying design box material can adopt polyethylene (PE), polypropylene (PP), polymethyl methacrylate (PMMA, be commonly called as lucite), Merlon (PC), polyurethane (PU), polytetrafluoroethylene (PTFE) (Teflon, PTFE), one or more in PETG (PET, PETE).Drier can be selected one or more of calcium chloride, calcium sulfate, magnesium chloride, silica gel, molecular sieve, montmorillonite.Deoxidier is selected one or more in technique of activated ferrous powder with, vitamin C, enzyme deoxidier and photaesthesia dyestuff.Macromolecular material selects natural, semi-synthetic and synthesized polymer material as PLA (PLA, PLLA), poly-alkanoic acid ester (PHAs), shitosan, alginic acid etc.
Using method: the biomolecule that needs are preserved, under sterile working state, is placed in sample reservoir vessel, and silk mouth bottle cap is tightened, and biological sample forms glassy state under the effect such as drier and deoxidier in auxiliary agent bag, preserves for a long time.
The present invention has the following advantages: 1, to design box material be the material with good biocompatibility in the present invention, in preservation process, can sample not produced and be polluted.2, because sample does not directly contact with auxiliary agents such as drier, can not cause damage to the molecule in sample.3, for doing for the sample of different test items while preserving respectively, can accomplish rapid saving.The present invention can be quick, stable under normal temperature condition the sample product for the treatment of preserve respectively.
Marginal data:
Below in conjunction with drawings and Examples, the present invention is further described.Wherein:
Fig. 1: liquid biological sample rapid draing box schematic diagram of the present invention: the 11 silk mouthful bottle caps with micro slide, 12 is the micro slide on silk mouthful bottle cap, and 13 is the brace of silk mouthful bottle cap and micro slide, and 14 is drier dividing plate, and 15 is air-vent, 16 is auxiliary agent bag.
Fig. 2: solid biologic sample rapid draing box schematic diagram of the present invention: 21 is silk mouthful bottle cap, and 22 is shuttle air-vent, and 23 is solid sample container, and 24 is solid sample, and 25 is auxiliary agent bag.
the specific embodiment:
Embodiment 1:
Quick-drying design box comprises: cylindrical bottle, and diameter is 35mm, height is 48mm; Circular wire mouth bottle cap 11, a silk mouthful bottle cap 11 contains a plurality of micro slides 12; Micro slide 12 is comprised of filter paper, the cavernous body with certain water absorbing capacity, contains trehalose, maltose, maltodextrin, HES etc. and a small amount of glycerine as the protective agent of biomolecule; A plurality of micro slides 12 interconnect with adhesive means by brace 13, and brace is connected with bottle cap 11, and connected mode is the methods such as gluing, clamping, clip; In order to prevent auxiliary agent, to the pollution of sample, can establish the division board 14 that diameter is identical with cylindrical bottle internal diameter; A plurality of air-vents 15 are set on division board 14, and air-vent is shaped as circle, ellipse or rectangle, in division board outer rim, is arranged in order; Auxiliary agent bag 16 is placed on bottle bottom.
Using method: the liquid bio molecule that needs are preserved is under sterile working state; drip respectively on the micro slide 12 on silk mouth bottle cap 11; now under the effect of the biomolecule protective agent moisture in biological sample on micro slide 12, be dissolved in biological sample; after being added dropwise to complete; silk mouth bottle cap 11 is tightened; biological sample forms glassy state under the effect of auxiliary agent bag, preserves for a long time.
Embodiment 2:
Quick-drying design box comprises: cylindrical bottle, and diameter is 35mm, height is 48mm; Circular wire mouth bottle cap 21; In bottle, be provided with groove shape solid sample container 23, its edge is slightly larger than bottleneck diameter, thereby is connected with bottleneck in the mode of clamping; Solid sample container 23 is provided with shuttle air-vent 22, is shaped as circle, ellipse or rectangle, at solids container sidewall, is from top to bottom staggered, and the solid sample 24 of collection can be placed in solid sample container 23; Auxiliary agent bag 25 is placed on bottle bottom.
Using method: the solid biologic sample 24 that needs are preserved, under sterile working state, is placed in solid sample container 23, tightens bottle cap 21, biological sample rapid draing under the effect of auxiliary agent bag, preserves for a long time.
Claims (8)
1. fast dewatering fixing biological molecules detects a device for sample, it is characterized in that, comprises following structure: cylindrical bottle, and diameter is 25～60mm, height is 35～75mm; Circular wire mouth bottle cap (11,21); In bottle or bottle cap, be provided with sample reservoir vessel (12,23), on described reservoir vessel (12,23), be placed with the protective agent of filter paper, cavernous body and the biomolecule with certain water absorbing capacity; Bottle bottom is placed with auxiliary agent bag (16,25); Between described auxiliary agent bag (16,25) and described sample reservoir vessel (12,23), there is spacer assembly (23,14); On described spacer assembly (23,14), be provided with air-vent (15,22).
2. dehydration fixing biological molecules as claimed in claim 1 detects the device of sample, it is characterized in that: described bottle material is one or more in polyethylene, polypropylene, polymethyl methacrylate, Merlon, polyurethane, polytetrafluoroethylene (PTFE), PETG.
3. dehydration fixing biological molecules as claimed in claim 1 detects the device of sample, it is characterized in that: described auxiliary agent bag (16,25) comprising: one or more drier, porous spongy foam and deoxidier;
Described drier is one or more of calcium chloride, calcium sulfate, magnesium chloride, silica gel, molecular sieve, montmorillonite;
Described deoxidier is one or more in technique of activated ferrous powder with, vitamin C, enzyme deoxidier and photaesthesia dyestuff.
4. dehydration fixing biological molecules as claimed in claim 3 detects the device of sample, it is characterized in that: deoxidier is inner at porous foam.
5. dehydration fixing biological molecules as claimed in claim 3 detects the device of sample, it is characterized in that: deoxidier is independent of porous foam outside.
6. dehydration fixing biological molecules as claimed in claim 1 detects the device of sample, it is characterized in that: protectant composition of biomolecule is trehalose, maltose, maltodextrin, HES and glycerine.
7. the arbitrary dehydration fixing biological molecules as described in claim 1～6 detects the device of sample, it is characterized in that: described air-vent (15,22) is circle, ellipse or rectangle.
8. described in a claim 1, fast dewatering fixing biological molecules detects the using method of sampling device, its step is as follows: the biomolecule that needs are preserved is under sterile working state, be placed in sample reservoir vessel (12,23), silk mouth bottle cap (11,21) is tightened.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310517752.0A CN103706411B (en) | 2013-10-25 | 2013-10-25 | A kind of fast dewatering fixing biological molecules detects the apparatus and method of sample |
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| CN201310517752.0A CN103706411B (en) | 2013-10-25 | 2013-10-25 | A kind of fast dewatering fixing biological molecules detects the apparatus and method of sample |
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| CN103706411A true CN103706411A (en) | 2014-04-09 |
| CN103706411B CN103706411B (en) | 2015-08-26 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8182747B2 (en) * | 2002-07-31 | 2012-05-22 | Roche Diagnostics Operations, Inc. | Test device for analyzing a biological sample liquid |
| CN102607918A (en) * | 2012-03-13 | 2012-07-25 | 张一意 | Body fluid specimen storage bottle |
| CN202715421U (en) * | 2012-09-10 | 2013-02-06 | 汕头大学医学院第一附属医院 | Medical sample conveying device |
| CN202814727U (en) * | 2012-10-10 | 2013-03-20 | 柯松华 | Collecting bottle |
| CN203803512U (en) * | 2013-10-25 | 2014-09-03 | 无锡灵锡医疗器械科技有限公司 | Device capable of rapidly dehydrating and fixing biomolecule detection sample |
-
2013
- 2013-10-25 CN CN201310517752.0A patent/CN103706411B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8182747B2 (en) * | 2002-07-31 | 2012-05-22 | Roche Diagnostics Operations, Inc. | Test device for analyzing a biological sample liquid |
| CN102607918A (en) * | 2012-03-13 | 2012-07-25 | 张一意 | Body fluid specimen storage bottle |
| CN202715421U (en) * | 2012-09-10 | 2013-02-06 | 汕头大学医学院第一附属医院 | Medical sample conveying device |
| CN202814727U (en) * | 2012-10-10 | 2013-03-20 | 柯松华 | Collecting bottle |
| CN203803512U (en) * | 2013-10-25 | 2014-09-03 | 无锡灵锡医疗器械科技有限公司 | Device capable of rapidly dehydrating and fixing biomolecule detection sample |
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| CN103706411B (en) | 2015-08-26 |
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Effective date of registration: 20170516 Address after: Pukou Tian Hua Bei Lu District of Nanjing City, Jiangsu province 210000 No. 6 Building 019 unit 802 room Patentee after: Sun Wenquan Address before: Xishan Economic Development Zone, Jiangsu province 214192 Technology Park in Wuxi city (three Furong Road No. 99) cloud five Patentee before: WUXI LINGXI MEDICAL DEVICES TECHNOLOGY CO., LTD. |
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Effective date of registration: 20190425 Address after: 250010 Port Xingsan, Jinan High-tech Zone, Shandong Province Patentee after: Yinfeng cryomedicine Technology Co. Ltd. Address before: Room 802, Unit 1, 019 Building No. 6 Tianbei Road, Pukou District, Nanjing City, Jiangsu Province Patentee before: Sun Wenquan |