CN103690582A - Composition containing dendrobium polysaccharide and atractylodes oil and application thereof - Google Patents
Composition containing dendrobium polysaccharide and atractylodes oil and application thereof Download PDFInfo
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Abstract
The invention provides a composition containing dendrobium polysaccharide and atractylodes oil and application thereof. The composition contains dendrobium polysaccharide, atractylodes oil and pharmaceutically acceptable auxiliaries for treating or preventing effective dose, and can be applied to preparation of medicines or health-care foods for preventing or relieving gastric ulcer.
Description
Technical field
The invention belongs to medicine and food compositions field, particularly, relate to a kind of medicine or food compositions containing dendrobium polysaccharide and atractylis concrete, and the preparation method of described compositions and treat and/or prevent the application in Gastric Ulcer Treatment or food in preparation.
Technical background
Chinese medicine Herba Dendrobii is the general name of the multiple medicinal plants of the orchid family (Orchidaceae) plant Dendrobium (Dendrobium).Herba Dendrobii begins to be loaded in Sheng Nong's herbal classic, and it has the function of nourishing the stomach to promote the production of body fluid, nourishing YIN and clearing away heat, improving eyesight, for cloudy impairment of body fluid, lose, and xerostomia excessive thirst, lack of appetite is retched, deficiency-heat after being ill, the symptoms such as poor vision.In decades, Chinese and overseas scholars has carried out a large amount of research to the chemical composition of Dendrobium Sw and pharmacological action, found that in Dendrobium Sw, chemical composition type is various; Pharmacological research proof Herba Dendrobii has defying age, antitumor, falls hypoglycemic effect, aspect treatment gastroenteropathy and treatment cataract, is also having good curative effect.
It is reported, approximately there are 63 kinds of Dendrobium Sws in China, is mainly distributed in south China and southwest, and be made for medicinal Dendrobium Sw approximately have kind more than 30 (Zhang Jili, He Jinli. Herba Dendrobii pharmacological research progress. time precious traditional Chinese medical science traditional Chinese medicines .2000; 11 (5): 469-470).< < Pharmacopoeia of People's Republic of China > > version in 2000 is recorded Herba Dendrobii for U.S. flower Herba Dendrobii (dendrobium loddigesii Rolfe; pharmacopeia is recorded name; lower with) the fresh or dry stem of D.loddigesii, Dendrobium fimbriatum Hook. (Herba Dendrobii oculati) D.fimbriatum (D.fimbriatum), Dendrobium chrysanthum (HERBA DENDROBII) D.chrysanthum, Herba Dendrobii D.officinale (D.candidum) and Herba Dendrobii (Dendrobium nobile) D.nobile (and Zhang Guangnong, etc. Advances in studies on chemical constitutents from plants of Dendrobium Sw. Chinese herbal medicine .2003; 34 (6): attached 5-8).
It is reported; the chemical constitution study of multiple Dendrobium Sw; found that the contained chemical composition type of this platymiscium is various; except alkaloid; also have luxuriant and rich with fragrance class, bibenzyl, Fluorenone class, sesquiterpenoids, coumarin, polysaccharide, steroidal and volatilization wet goods (Zhang Guangnong, etc. Advances in studies on chemical constitutents from plants of Dendrobium Sw. Chinese herbal medicine .2003; 34 (6): attached 5-8).
Dendrobium polysaccharide refers to from Dendrobium plant and extracts the polysaccharide compound obtaining.Studies have reported that, dendrobium polysaccharide there is immunomodulating and regulate the activity of blood glucose (Liu Li, etc. Dendrobium medicinal plant polysaccharide progress. R&D of modern TCM with put into practice .2009; 23 (1): 77-80).
According to < < Chinese Pharmacopoeia > > (2010 editions), Rhizoma Atractylodis refer to the dry rhizome of Compositae [WTBX plant Atractylodes lancea (Thunb.) DC. (RHIZOMA ATRACTYLODIS IANCEAE) Atractylodeslancea, Atractylis chinensis Atractylodeschinensis.In addition, the congener using as medicinal plants also comprises atractylodes japonica Atractylodes japonica, Atractylodes koreana A.koreana, full leaf Rhizoma Atractylodis A.chinensis, the Rhizoma Atractylodis A.chinensis of Chifeng.
The Rhizoma Atractylodis Chinese medicine of existing thousands of years tradition applicating history.Chinese Traditional Medicine document is recorded, and Rhizoma Atractylodis acrid in the mouth, hardship are warm in nature, return spleen, stomach, Liver Channel, have drying damp and strengthening spleen, wind-damp dispelling, the effect of improving eyesight.Be mainly used in distension and fullness in the abdomen, have loose bowels, edema, beriberi flaccidity syndrome and arthralgia syndrome, rheumatic arthralgia, anemofrigid cold, nyctalopia nyctalopia.
Atractylis concrete, claims again Rhizoma Atractylodis oil (Atranctyllis oil), is a kind of volatile oil obtaining that extracts from Chinese medicine Rhizoma Atractylodis dry rhizome.Conventional Rhizoma Atractylodis oil extracting method is steam distillation, and its yield is about 3%～8%.In food and daily chemicals, atractylis concrete is used mainly as fixastive.
Chinese medicine is learned and is thought that Rhizoma Atractylodis are dry; parched with bran is one of main medicinal material processing method of Rhizoma Atractylodis, it is generally acknowledged, the effect of parched with bran is the volatile oil content that reduces medical material; reduce its " property is dry " characteristic (Xu Dechun, etc. Rhizoma Atractylodis are concocted primary research at all times. time precious traditional Chinese medical science traditional Chinese medicines .2001; L2 (3): 257-258).
Have bibliographical information, Rhzoma Atractylodis Lanceae volatile oil has antibacterial sterilization functions, can regulate the gastrointestinal movement function of laboratory animal.
Gastric ulcer is modal a kind of in peptic ulcer, mainly refers to the tissue injury that surpasses muscularis mucosae that gastric mucosa is caused by peptic digestion liquid autodigestion.Along with increase and the diet of people's social pressure are irregular, increasing people suffers from chronic gastric ulcer, and except minority is compared with the patient of Seek early medical advice, the most of patients course of disease is several years, more than ten years or longer time, and relapse rate is high.
Modern medicine study thinks, the main pathological factor of gastritis and gastric ulcer comprises gastroxia, Helicobacter pylori infection etc.Wherein, it is the Etiological of chronic gastritis that helicobacter pylori (Helicobacter pylori, HP) infects, and is the Important cause of disease that causes peptic ulcer.The epithelial cell microvilli adhering at HP reduces, and iuntercellular connects to be lost, cellular swelling, surface imperfection, and in cell, MG is exhausted, and cavity sample becomes, and antibacterial and iuntercellular form the adhesion base of a fruit and tazza spline structure.
Modern medicine is increasingly deep to the cause of disease of gastritis, gastric ulcer and pathological research, and doctor trained in Western medicine has improved the treatment effect to gastritis, peptic ulcer significantly.The treatment measure of peptic ulcer comprises feelings will, dietary adjustments and Drug therapy.Antacid can lower the acidity of Stomach duodenum as sodium bicarbonate, gel aluminum hydroxide, bismuth subcarbonate etc., and alleviating pain, promotes ulcer healing.Anticholinergic atropine, propantheline, metoclopramide etc. can suppress vagus nerve and reduce gastric acid secretion, but it delays gastric emptying and can cause that gastrin secretion increases.Histamine H2-receptor antagonist cimetidine, the competition H of famotidine Deng Yu histamine
2receptor, eliminates histamine and causes gastric acid secretion effect.Acid pump inhibitor forms triple therapy as metronidazole and Li Fuxisheng as omeprazole and lansoprazole and inhibition helicobacter pylori medicine, to treat the peptic ulcer disease of the helicobacter pylori positive.
The clinical manifestation of gastritis, gastric ulcer belongs to the categories such as Chinese medicine " noisy ", " stomach painful abdominal mass ", " acid regurgitation ", " gastric abscess ", " pained ".The doctor trained in Western medicine exquisite symptomatic treatment of curing the disease, it is main with repairing ulcer face, diminishing inflammation, kill the measures such as helicobacter pylori, drug treatment is long, and side effect is large.The traditional Chinese medical science is thought spleen and stomach phase the exterior and the interior, and The spleen is in charge of sending up essential substances, and stomach master is respectful to fall, and incoordination between the spleen and stomach or irritability are cut down the pathogenesis such as stomach all can cause gastropathy, adopts the methods such as the spleen and stomach regulating, catharsis irritability can treat on the whole gastropathy.
So far, in prior art, there is not the report of the compositions that atractylis concrete and dendrobium polysaccharide are mixed with, can not produce the report of collaborative antiulcer action with there is no said composition.
Summary of the invention
The object of the present invention is to provide a kind of compositions that atractylis concrete and dendrobium polysaccharide are mixed with, the present invention mixes the atractylis concrete of molten condition with dendrobium polysaccharide, under room temperature, make solid, shaped mixture, gained complex can produce collaborative antiulcer action, particularly the Experimental Gastric Ucler model due to helicobacter pylori is had to significant preventive and therapeutic action.In addition, the present invention is also to provide the preparation method of said composition and treats and/or prevents the application in Gastric Ulcer Treatment or food in preparation.
Following technical proposals of the present invention is for realizing above-mentioned goal of the invention:
First the present invention provides a kind of edible and/or Pharmaceutical composition, and described compositions contains the upper acceptable excipient of dendrobium polysaccharide extract, atractylis concrete and physiology that prevents and/or treats gastric ulcer effective dose, wherein:
Described dendrobium polysaccharide is from Herba Dendrobii (Dendrobiumcandidum) and/or Dendrobium nobile (Dendrobiumnobile), to extract the total polysaccharides obtaining, and in described dendrobium polysaccharide extract, carbohydrate content content is not less than 80% of extract total amount, and polysaccharide weight average molecular weight is not less than 20kD;
Described atractylis concrete is from Rhizoma Atractylodis Atractylodeslancea dry rhizome, to extract the total volatile oil obtaining, and wherein the content sum of atractylol and eudesmol is not less than 50% of volatile oil total amount;
The proportion of composing of described dendrobium polysaccharide and atractylis concrete is in the scope of 1:0.25 to 1:2.
Edible and/or Pharmaceutical composition of the present invention refers to that compositions of the present invention can be used as functional food, also can be used as prevention and treats the pharmaceutical composition of peptic ulcer.
Dendrobium polysaccharide of the present invention can optionally extract from Herba Dendrobii Dendrobiumcandidum and/or Dendrobium nobile Dendrobiumnobile.The present invention studies and shows, Dendrobium officinale polysaccharide and Dendrobium nobile polysaccharide can produce approximate anti-gastric-ulcer activity for the present invention's pharmaceutical composition.
In the [WTBX plant drying rhizome of different plant species the chemical composition of contained volatile oil form and to differ greatly (Wang Liuying, etc. analysis and the comparison of Rhizoma Atractylodis class medical material volatile ingredient. time precious traditional Chinese medical science traditional Chinese medicines 2009; 20 (4): 924-925).Atractylis concrete of the present invention refers to the volatile oil that claims again to extract RHIZOMA ATRACTYLODIS IANCEAE Atractylodeslances dry rhizome acquisition from Atractylodes lancea (Thunb.) DC..In described atractylis concrete, in weight ratio, the content sum of atractylol (hinesol) and eudesmol (β-eudesmol) is not less than 50% of volatile oil total amount.Atractylol (hinesol) and eudesmol (β-eudesmol) are as shown below.
The atractylol that atractylis concrete is contained and β-eudesmol can be confirmed by chromatography of gases-GC-MS, and its content can detect by gas chromatography.
The present invention studies discovery, and the proportion of composing of dendrobium polysaccharide and atractylis concrete is in the scope of 1:0.25～1:2 time, and it is active that described compositions all has good collaborative anti-experimental character gastric ulcer.In the preferred embodiment of the invention, the dendrobium polysaccharide described in it in pharmaceutical composition and the proportion of composing of atractylis concrete are in the scope of 3:0.5～1:1.
The present invention also provides the preparation method of described dendrobium polysaccharide, and described method comprises the steps:
(1) water extraction method obtains Herba Dendrobii water extract;
(2) aqueous solution of step (1) gained Herba Dendrobii water extract is obtained to Herba Dendrobii total polysaccharides through C1-C4 lower alcohol or ketone fractional precipitation;
(3) the dendrobium polysaccharide extract that can further optionally adopt decolouring, ultrafiltration or chromatography purification to obtain step (2) gained Herba Dendrobii total polysaccharides.
In its step (1), it is generally the water with approximately 8～15 times of weight that described water extraction extracts, and under room temperature to 100 ℃ condition, extracts 2～3 times; After can optionally obtained aqueous solution suitably being concentrated, be for further processing;
In step (2), preferred rudimentary alcohol ketone comprises ethanol and acetone, and gained precipitation can optionally adopt rudimentary alcohol ketone to wash after 1～3 time, adopts drying under reduced pressure, cryodesiccated method to be dried.
Step (2) gained total polysaccharides can be directly used in the preparation of the present composition.It will be understood by those skilled in the art that described total polysaccharides also can further adopt decolouring, ultrafiltration, gel chromatography, ion exchange chromatography to be further purified.
The repeated trials result of study repeatedly according to the present invention, the neutral sugar content in gained dendrobium polysaccharide extract is not less than 80% of total extract; Efficient gel chromatography (HPGPC) testing result shows, impact due to raw material sources (manufacturer and production batch are different) and degree of purification, the component of gained dendrobium polysaccharide is not quite similar, but generally, its mean molecule quantity is all not less than about 20kD.
The present invention also further provides the preparation method of atractylis concrete, described method can optionally adopt (1) steam distillation, (2) supercritical extraction, (3) organic solvent extraction method to extract and obtain total volatile oil, i.e. atractylis concrete of the present invention.
Described essential oil extraction method is well known to those skilled in the art, and wherein, steam distillation is conventional essential oil extraction method; And CO
2supercritical extraction can improve the extraction yield of volatile oil, in its product, may contain the fatty acid ester of high level, the methods such as further employing crystallization, solvent extraction that it will be understood by those skilled in the art that can reduce or part removes fatty acid in supercritical extraction product and the content of ester thereof; Rudimentary property organic solvent also can obtain atractylis concrete required for the present invention as cyclohexane.
The present invention also provides the solid preparation preparation method of described pharmaceutical composition, and its step comprises:
At (1) 60 ℃～80 ℃, make the atractylis concrete of amount of formulation be molten condition, stir the dendrobium polysaccharide that adds amount of formulation ,-18 ℃～4 ℃ cold preservations make it to solidify, pulverize at low temperature;
(2) in step (1) products therefrom, add the upper acceptable excipient of physiology and granulate;
(3) by step (2) gained granule tabletting or filled capsules.
Generally speaking, plant volatile oil constituents, due to its grease character, is prepared into soft capsule class preparation more, the more difficult solid preparation that is prepared into.The present invention finds, dendrobium polysaccharide is uniformly mixed in the atractylis concrete under molten condition, in the situation that reducing temperature, can obtain the solid content that is easy to pulverizing, after its solid content is pulverized, can be easily and pharmaceutically acceptable mixed with excipients, and gained mix powder or granule can have good mobility, be easy to granulate with pharmaceutical adjunct, be therefore easy to filled capsules or be pressed into tablet.
Described pharmaceutically acceptable excipient can include but not limited to filler, slip agents, binding agent, coloring agent etc.For solid tablet, also can optionally adopt the coatings such as coating materials, coloring agent.
The present invention studies have shown that, of the present invention has the effect of the ulcer of preventing and/or treating containing dendrobium polysaccharide atractylis concrete compositions in the gastric ulcer model due to pylorus ligation, helicobacter pylori, and its effect presents obvious synergism effect, therefore of the present inventionly further provide pharmaceutical composition of the present invention to prevent and/or treat the purposes in Gastric Ulcer Treatment in preparation.
Accompanying drawing explanation
Fig. 1 is efficient gel chromatograph (HPGPC) collection of illustrative plates of Dendrobium officinale polysaccharide and Dendrobium nobile polysaccharide, (A: Dendrobium officinale polysaccharide; B: Dendrobium nobile polysaccharide);
Fig. 2 is atractylis concrete GC-MS spectrogram, (A:GC spectrogram; B: atractylol MS spectrum; C: β-eudesmol MS spectrum).
The specific embodiment
The present invention, below in conjunction with accompanying drawing, is described further essentiality content of the present invention with specific embodiment.But these specific embodiments only, for the present invention is described, do not constitute any limitation scope of the present invention.
Embodiment 1:
Dendrobium polysaccharide preparation
Get Herba Dendrobii Dendrobiumcandidum and each 1000g of Dendrobium nobile Dendrobiumnobile dry aerial parts, the defat of 95% (V/V) soak with ethanol, the centrifugal ethanol that goes, at 90 ℃, 12L water extraction is twice, merge aqueous extract, be evaporated to the about 8L of extracting solution cumulative volume, add 24L95% (V/V) ethanol, standing over night at 4 ℃, centrifugal must precipitation.With 3L pure water, dissolve gained precipitation respectively, the centrifugal insoluble matter that goes, supernatant is adjusted pH2.8 with 2N HCl, standing about 4hr at 4 ℃, the centrifugal precipitation of going, 2N NaOH adjusts pH9, adds 90ml30%H
2o
2, stir at 60 ℃ after decolouring 1hr, add 9L95% (V/V) ethanol, standing over night at 4 ℃, centrifugal must precipitation, gained precipitate with 2L90% washing with alcohol twice, and under room temperature, drying under reduced pressure respectively must the about 132.7g of total polysaccharides and 38.5g.
Get Herba Dendrobii total polysaccharides 80g and be dissolved in 2000ml pure water, 3kD ultrafilter membrane (Millipore ultrafiltration apparatus) ultrafiltration, holds back solution lyophilization and obtains Dendrobium officinale polysaccharide 52.6g;
Separately get Dendrobium nobile total polysaccharides 25g, be dissolved in 800ml pure water, cross Sephadex-G25 gel chromatographic columns (Ф 8cm x150cm) purification, collect containing after saccharic composition, lyophilization obtains Dendrobium nobile total polysaccharides 15.3g.
The sugared content that phenol sulfuric acid method detects in described Herba Dendrobii total polysaccharides, Dendrobium officinale polysaccharide, Dendrobium nobile total polysaccharides, Dendrobium nobile polysaccharide is respectively 81.3%, 88.7%, and 82.6% and 92.8%.
Take standard glucosan as standard, and efficient gel chromatograph (HPGPC) method detects described Dendrobium officinale polysaccharide and Dendrobium nobile polysaccharide, and it is multi-component mixing polysaccharide as seen, and the weight average molecular weight of polysaccharide component is all greater than 20kD (seeing Figure 1A, B).
Embodiment 2:
Atractylis concrete preparation
Method: get three parts of each 1kg of Atractylodes lancea (Thunb.) DC. Atractylodes Lances dry rhizome granule that pulverized, be respectively used to vapor distillation extraction, organic solvent extraction and CO
2supercritical extraction.Wherein:
(1) to extract be that 1kg Atractylodes lancea (Thunb.) DC. dry rhizome granule is inserted in the port grinding bottle of volatile oil extractor to vapor distillation, by Chinese Pharmacopoeia appendix method continuous still 5 hours, then stops heating, discharge water after cooling, and the grease on collection distillate surface.
(2) organic solvent extraction method is that 1kg Atractylodes lancea (Thunb.) DC. dry rhizome granule is inserted in flask, usings normal hexane as solvent, reflux, extract, 8h, and distilling under reduced pressure, except desolventizing, obtains extracting product.
(3) CO
2supercritical extraction is that 1kg Atractylodes lancea (Thunb.) DC. dry rhizome granule is put into supercritical fluid extraction device, flow～35kg/h, static extracting 1hr, dynamic extraction 3hr under temperature～50 ℃, pressure～20MPa.At separating pressure～10MPa, at 40 ℃ of temperature, separate out volatile oil.
Gc analysis method (GC) detects the content of atractylol and β-eudesmol in three kinds of extraction method gained atractylis concretes, and the chromatographic condition of described detection is, HP-5MS chromatographic column, quartz capillary column (μ m * 0.25,30m * 250 μ m); 280 ℃ of vaporizer temperature; Mass spectrum (MS) detector, 230 ℃ of ion source temperatures; 200 ℃ of quadrupole rod temperature; Column temperature (temperature programming): 60 ℃ (3min), 10 ℃/min is warming up to 150 ℃, and 3 ℃/min is warming up to 220 ℃, and 25 ℃/min is warming up to 280 ℃ (10min); Split ratio 10:1; Carrier gas He, flow 1.0ml/min.
Result: steam distillation obtains the about 32.5g of atractylis concrete, and products therefrom at low temperatures (20 ℃ or following) is light yellow decorating film.Gas chromatography detects, and the content sum of its atractylol and β-eudesmol is approximately 83.6% (wherein containing atractylol approximately 48.8%, containing β-eudesmol approximately 34.8%).The GC-MS collection of illustrative plates of steam distillation gained atractylis concrete as shown in Figure 2.Wherein Fig. 2 A is that GC detects gained total ion current spectrogram; 2B is atractylol MS spectrum; 2C is β-eudesmol MS spectrum.
Normal hexane extraction method obtains the about 25.1g of atractylis concrete, the yellow oil that products therefrom is thickness.Gas chromatography-mass spectrography (GC-MS) detects, and the content sum of its atractylol and β-eudesmol is approximately 54.2%;
CO
2supercritical extraction obtains the about 41.6g of atractylis concrete, and products therefrom at low temperatures (20 ℃ or following) is light yellow decorating film.Gas chromatography-mass spectrography (GC-MS) detects, and the content sum of its atractylol and β-eudesmol is approximately 69.2%.
Embodiment 3:
The therapeutical effect of dendrobium polysaccharide atractylis concrete compositions to pyloric ligation ulcers laboratory animal gastric ulcer model:
Test specimen: dendrobium polysaccharide; Atractylis concrete (extraction by steam distillation); Compositions A, it is the weight ratio Dendrobium officinale polysaccharide that is 1:0.5 and the mixture of steam distillation gained atractylis concrete; Compositions B, it is the mixture of the atractylis concrete of the weight ratio Dendrobium nobile polysaccharide that is 1:2 and normal hexane extraction; Compositions C, it is the mixture of the atractylis concrete of the weight ratio Dendrobium nobile polysaccharide that is 1:0.25 and supercritical extraction.
Test method: 64 of body weight 180～220g SD rats, be divided at random 8 groups (8 every group), it is model control group, dendrobium polysaccharide administration group, atractylis concrete administration group, dendrobium polysaccharide-Rhizoma Atractylodis water volatile-oil composite administration group (establishing respectively A-200mg/kg, A-100mg/kg, B-200mg/kg, C-100mg/kg administration group), positive controls (ranitidine-50mg/kg).
Test rat fasting in first 48 hours and can't help water.During test, after rat anesthesia is fixing, sterile working's ligation pylorus.Postoperative each test group animal is the corresponding tested medicine of gavage respectively, positive controls gavage ranitidine, and model group gives the solvent of equivalent by the same method, and it is for food to stop supplying water.
After 18 hours, put to death rat, open abdominal cavity, nearly diaphragm place's ligation esophagus, gets stomach and fixes 10 minutes in 1% formalin.Along greater gastric curvature, cut coat of the stomach, clean content, launch coat of the stomach and be fixed on stencil plate with pin, observe gastric mucosa face, record according to a conventional method every group of ulcer number of animals, ulcer area and ulcer index.
Result of the test: experimental result is in Table 1.
Result shows, with model group comparison, the Mus number of each administration group generation ulcer reduces to some extent, ulcer level significantly alleviates, and ulcer area is dwindled, and ulcer index reduces.
The antiulcer result demonstration of compositions A, the pharmacodynamic action of dendrobium polysaccharide-atractylis concrete compositions is obviously better than corresponding dendrobium polysaccharide, the individually dosed drug effect of Rhizoma Atractylodis water volatile oil, and its drug effect has the pharmacodynamics effect of Synergistic.The pharmacodynamic experiment result of compositions B and compositions C shows, Dendrobium officinale polysaccharide and Dendrobium nobile polysaccharide coupling atractylis concrete all can produce synergistic function; Vapor distillation, supercritical extraction and organic solvent extraction gained atractylis concrete all have antiulcer action.
Table 1. rat Pylorus Ligated Gastric Ulcer animal model test result
Embodiment 4:
Dendrobium polysaccharide atractylis concrete compositions causes the therapeutical effect of rat gastric ulcer model to helicobacter pylori:
Test specimen: dendrobium polysaccharide atractylis concrete compositions D, it is the weight ratio Dendrobium officinale polysaccharide that is 1:1 and the mixture of steam distillation gained atractylis concrete.
Test method: 40 of body weight 180～220g SD rats.Rat fasting 24hr, after pentobarbital anesthesia, cuts open the belly, and with diameter 5.5mm filter paper, dips glacialacetic acid dipping gastric antrum portion serosal surface twice, each 30s, and normal saline cotton balls is put on the skin and is washed the position 3 times of burning, stitching abdominal cavity, postoperative general nursing.Postoperative 3 days, gavage helicobacter pylori HP10 after rat fasting 24hr
9cFUP/ only, continuous four day afterwards every day gavage HP, totally 5 times.After modeling, rat is divided into 4 groups (10 every group) at random, i.e. model control group, the compositions D administration group of 250mg/kg and 125mg/kg dosage, positive controls (bismuth potassium citrate sheet/tinidazole tablets/clarithromycin tablet, 75mg/kg).The continuous gastric infusion of each administration group 7 days, model group gives equal-volume normal saline.Within after last perfusion 2 weeks, put to death animal, get stomach and observe and calculate ulcer index; Get gastric antrum portion tissue simultaneously and do the existence of urease dependence test (RUT) checking experiment animal Hp.
Experimental result: dendrobium polysaccharide atractylis concrete compositions D infects and has significant inhibitory action HP, and burn-HP of glacial acetic acid infective ulcer model is had to good therapeutical effect.
Table 1. rat Pylorus Ligated Gastric Ulcer animal model test result
Embodiment 5:
The solid tablet preparation of dendrobium polysaccharide atractylis concrete compositions:
Prescription
Method for making: the Rhizoma Atractylodis oil of melting at 60 ℃, in stirring, add dendrobium polysaccharide, place at 0 ℃ and spend the night, gained solid content is pulverized (80 order), the microcrystalline Cellulose mixing granulation that adds recipe quantity, gained granule adds recipe quantity magnesium stearate and mixes, and conventional method is suppressed tablet in blocks.
Embodiment 5:
The hard capsule preparation of dendrobium polysaccharide atractylis concrete compositions:
Prescription
Method for making: the atractylis concrete of the recipe quantity of melting at 60 ℃, the polyvinylpyrrolidone (PVP K90) and the dendrobium polysaccharide that in stirring at 60 ℃, add recipe quantity, after continuing 30min and stirring, places at 0 ℃ and spend the night, gained solid content pulverizing (80 order), add recipe quantity lactose, microcrystalline Cellulose mix homogeneously, add the HPMC solution preparing in advance to make soft material, 20 mesh sieves are granulated, and 60 ℃ are dried approximately 30 minutes, 18 mesh sieve granulate, fill 2
#capsule.
Above-described embodiment 5 and 6 Tablet and Capsula agent also can be for food (health foods).
Claims (7)
1. a compositions, the upper acceptable excipient of dendrobium polysaccharide, atractylis concrete and the pharmacology of containing treatment or preventing gastric ulcer effective dose, wherein:
Described dendrobium polysaccharide is that the content that extracts from Herba Dendrobii Dendrobiumcandidum and/or Dendrobium nobile Dendrobiumnobile is not less than the total polysaccharides that extract total amount 80%, polysaccharide weight average molecular weight are not less than 20kD;
Described atractylis concrete is the total volatile oil extracting from Rhizoma Atractylodis Atractylodeslancea, and wherein the content sum of atractylol and eudesmol is not less than 50% of volatile oil total amount;
The composition weight percent of described dendrobium polysaccharide and atractylis concrete is in the scope of 1:0.25 to 1:2.
2. compositions as claimed in claim 1, the composition weight percent of wherein said dendrobium polysaccharide and atractylis concrete is in the scope of 1:0.5 to 1:1.
3. the compositions as described in claim 1～2, wherein the preparation method of dendrobium polysaccharide comprises:
(1) water extraction method obtains Herba Dendrobii water extract;
(2) aqueous solution of step (1) gained Herba Dendrobii water extract is obtained to Herba Dendrobii crude polysaccharides through C2-C4 lower alcohol or ketone fractional precipitation;
(3) step (2) gained Herba Dendrobii crude polysaccharides is decoloured, ultrafiltration or chromatography purification obtain dendrobium polysaccharide.
4. the compositions as described in claim 1～2, wherein the preparation method of atractylis concrete is steam distillation or supercritical extraction or organic solvent extraction method.
5. the solid preparation preparation method of compositions described in claim 1 or 2, comprising:
At (1) 60 ℃～80 ℃, make the atractylis concrete of preparation effective dose be molten condition, stir the dendrobium polysaccharide that adds amount of formulation ,-18 ℃～4 ℃ cold preservations make it to solidify, pulverize at low temperature;
(2) in step (1) products therefrom, add the upper acceptable pharmaceutical excipient of pharmacology and granulate;
(3) by step (2) gained granule tabletting or filled capsules.
6. the application of compositions in the medicine of preparation prevention or treatment gastric ulcer described in claim 1 or 2.
7. the application of compositions in preparation prevention or treatment gastric ulcer functional food described in claim 1 or 2.
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| CN201310680378.6A Expired - Fee Related CN103690582B (en) | 2013-12-12 | 2013-12-12 | A kind of compositions and application thereof containing dendrobium polysaccharide and atractylis concrete |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106943450A (en) * | 2017-04-28 | 2017-07-14 | 苏州半部堂生物科技有限公司 | Containing astragalus polyose, the composition of atractylis concrete, preparation method and its usage |
| CN107149613A (en) * | 2017-04-28 | 2017-09-12 | 苏州半部堂生物科技有限公司 | Containing soluble polysaccharide, the composition of atractylis concrete, preparation method and its usage |
| CN112022966A (en) * | 2020-10-10 | 2020-12-04 | 中国药科大学 | Traditional Chinese medicine composition for preventing, relieving and/or treating hyperuricemia |
| CN112426525A (en) * | 2020-11-27 | 2021-03-02 | 浙江康佰裕生物科技有限公司 | A soluble composition for stimulating T cell activation and expansion and having synergistic anti-tumor effect on T cells, and its preparation method |
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| CN101703738A (en) * | 2009-11-11 | 2010-05-12 | 刘福启 | Chinese medicament for treating gastritis and gastric ulcer |
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| CN101703738A (en) * | 2009-11-11 | 2010-05-12 | 刘福启 | Chinese medicament for treating gastritis and gastric ulcer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106943450A (en) * | 2017-04-28 | 2017-07-14 | 苏州半部堂生物科技有限公司 | Containing astragalus polyose, the composition of atractylis concrete, preparation method and its usage |
| CN107149613A (en) * | 2017-04-28 | 2017-09-12 | 苏州半部堂生物科技有限公司 | Containing soluble polysaccharide, the composition of atractylis concrete, preparation method and its usage |
| CN112022966A (en) * | 2020-10-10 | 2020-12-04 | 中国药科大学 | Traditional Chinese medicine composition for preventing, relieving and/or treating hyperuricemia |
| CN112426525A (en) * | 2020-11-27 | 2021-03-02 | 浙江康佰裕生物科技有限公司 | A soluble composition for stimulating T cell activation and expansion and having synergistic anti-tumor effect on T cells, and its preparation method |
| CN112426525B (en) * | 2020-11-27 | 2023-09-01 | 浙江康佰裕生物科技有限公司 | A soluble composition for stimulating T cell activation and proliferation and having synergistic T cell anti-tumor effect, and its preparation method |
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| CN103690582B (en) | 2016-03-30 |
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