CN103689267B - A kind of production method and nutritional agents of high DHA low cholesterol eggs nutritional agents - Google Patents
A kind of production method and nutritional agents of high DHA low cholesterol eggs nutritional agents Download PDFInfo
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Abstract
The invention belongs to Feed Manufacturing field, relate in particular to a kind of production method of producing high DHA low cholesterol eggs nutritional agents, nutritional agents and the feed that contains nutritional agents. A kind of production method of producing high DHA low cholesterol eggs nutritional agents: it is characterized in that: comprise following production stage: marine alga is fermented, obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents. The high DHA low cholesterol eggs nutritional agents of a kind of production, by fermentation of seaweed product, fish oil, linseed oil composition. Beneficial effect of the present invention is: by nutritional agents raw material is carried out to probiotics fermention, utilize the biology enzyme in sweat to transform, the DHA in nutrition fortifier is enriched in birds, beasts and eggs more efficiently; DHA content 420mg/100g in egg, improves more than 50 times than common egg; Cholesterol content in eggs≤290mg/100g is lower by 57% than common egg (cholesterol level 680mg/100g).
Description
Technical field
The invention belongs to Feed Manufacturing field, relate in particular to a kind of production method of producing high DHA low cholesterol eggs nutritional agents, nutritional agents and the feed that contains nutritional agents.
Background technology
Along with improving constantly of human living standard, people's health care consciousness is more and more stronger, also more and more to the demand of health food, makes developing green functional health-care food become the important directions of modern farming research. DHA(Docosahexaenoicacid, i.e. DHA, is commonly called as " docosapentaenoic acid ") belonging to a member in bunch polyunsaturated fatty acid of ω-3, it is one of chief component material of human brain, accounts for 10% left and right of people's cephalopin.
Human body can not synthesize DHA, must from food, absorb. Terrestrial life is not containing DHA, and natural DHA only exists in the planktonic organisms such as Diatomeae, eaten the rear absorption storage of digestion in vivo by food chain by the raw fish in sea, and the main source of mankind DHA is ocean fish/ocean fish oil/algal oil and manufacture thereof at present.DHATo protection and raising children/student and brain worker's intelligence and eyesight; to pregnant and lying-in women, fetus, infant, student, the elderly, vegetarian, to brain function lowly subtract, cognitive function decline, depression, high fat of blood, cardiovascular and cerebrovascular so anticancer etc. all have widely, indispensable and irreplaceable nutrition recuperating effect. The World Health Organization (WHO) in 1999 and international aliphatic acid and lipoid EASD (ISSFAL) formally announce to the whole world DHA substantially the standard of supplementing be: 220mg/ days is grown up. But, when people rely on Poultry and Eggs to supplement DHA, the same cholesterol of worrying hyperabsorption, thereby, develop a kind of high DHA low cholesterol eggs nutritional agents, be those skilled in the art's problem demanding prompt solutions, be of great significance and wide application prospect for the general level of the health and the intelligence tool that promote the mankind.
At present, relevant scholar has done some R&D works to the DHA content improving in birds, beasts and eggs both at home and abroad, substantially all concentrate on to the material that adds high DHA content in feed, thereby bird is enriched to the DHA content in feed in egg, but, because the DHA conversion ratio in feed or additive is difficult to control, the DHA content in egg improves not remarkable.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of production method of producing high DHA low cholesterol eggs nutritional agents, nutritional agents and the feed that contains nutritional agents, this nutritional agents can significantly improve the content of DHA in birds, beasts and eggs.
A kind of production method of producing high DHA low cholesterol eggs nutritional agents: it is characterized in that: comprise following production stage: marine alga is fermented, obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents.
A kind of production method of producing high DHA low cholesterol eggs nutritional agents: it is characterized in that: described fermentation is carried out in accordance with the following steps, obtains tunning;
(1) prepare shake-flask seed liquid: taking saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio is 3:1:1, under aseptic condition, starting strain is inoculated in fluid nutrient medium, cultivate 16-18h, obtain shake-flask seed liquid;
(2) prepare saccharomycete seed liquid: shake-flask seed liquid by volume, with the inoculum concentration of 2-5%, under aseptic condition, is inoculated in 50L seeding tank, cultivate 14-16h, fermentation obtains saccharomycete seed liquid;
(3) fermented and cultured: the saccharomycete seed liquid that step 2 is obtained, be by volume inoculated in 500L fermentation tank with 5-20%, between yeast phase, frequently add marine alga, the interpolation quality of marine alga is the 10-20% of fermentation cumulative volume, cultivate 24-30h, stop tank tapping, obtain tunning.
Wherein, described marine alga is preferably rich in the algae of DHA; Described marine alga is 20-60 order powder, process pasteurization before fermentation.
Wherein, described marine alga is preferably any one or a few in chlorella, sargassum thunbergii, Freshwater Chrysophytes.
Wherein, in step (1), inoculum concentration is the 4-10% of fluid nutrient medium volume, and shake-flask seed medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, and dipotassium hydrogen phosphate 0.3%, 20%NaOH solution regulates pH=7.5; Shake-flask seed condition of culture is preferably: temperature 32-37 DEG C, and 120-200rpm, liquid amount is 140-200mL;
In step (2), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Saccharomycete seed liquid condition of culture is: 37 DEG C, and mixing speed 200-250rpm, liquid amount is 30L-50L, throughput 2.0-2.5L/h;
In step (3), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Fermentation condition is preferably: temperature 32-37 DEG C, and mixing speed 100-150rpm, liquid amount is 300-500L, throughput 2.0-3.0L/h.
Wherein, it is characterized in that: described culture medium all passes through moist heat sterilization preferably 121 DEG C of sterilising conditions, autoclaving 20-30min.
A kind of produce high DHA low cholesterol eggs nutritional agents: it is characterized in that: described nutritional agents is by fermentation of seaweed product, fish oil, linseed oil composition.
Wherein, described fermentation of seaweed product accounts for the 10-30% of total nutritional agents weight, and fish oil accounts for the 20-50% of total nutritional agents weight, the 20-50% that accounts for total nutritional agents weight of linseed oil, and nutritional agents gross weight is 100 parts.
A kind of feed of producing high DHA low cholesterol eggs nutritional agents that contains: it is characterized in that: described feed optimization formula is as follows: corn flour accounts for the 60-70% of feed gross weight, dregs of beans accounts for the 20-30% of feed gross weight, oyster shell whiting accounts for the 5-10% of feed gross weight, calcium monohydrogen phosphate accounts for the 1-2% of feed gross weight, vitamin accounts for the 1-2% of feed gross weight, mineral matter accounts for the 1-2% of feed gross weight, and nutritional agents accounts for the 0.5-2% of feed gross weight.
Wherein, the bird that described feed is preferably fed is any one in chicken, duck, goose.
Starting strain in the present invention goes down as found activity, can carry out rejuvenation of spawn, and condition is as follows: culture medium: glucose 0.5%, beef extract 0.5%, yeast extract 0.2%, peptone 1.0%, dipotassium hydrogen phosphate 0.3%, agar 0.18%, 20%NaOH solution regulates pH=7.5; Condition of culture: cultivate 24h for 37 DEG C.
Beneficial effect of the present invention is: 1, by nutritional agents raw material is carried out to probiotics fermention, utilize the biology enzyme in sweat to transform, the DHA in nutrition fortifier is enriched in birds, beasts and eggs more efficiently; 2, feed nutrition agent harmless, without residual, pollution-free, easy to use, be easy to promote; 3, produce and meet NY5039-2005 standard non-polluted egg, in egg, (containing egg white and yolk) DHA content >=420mg/100g, improves more than 50 times than common egg; 4, cholesterol content in eggs≤290mg/100g is lower by 57% than common egg (cholesterol level 680mg/100g).
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further detailed explanation.
The bacterial strain that the present invention selects is purchased from Institute of Micro-biology of the Chinese Academy of Sciences, saccharomycete (bacterium numbering 2.540), bacillus subtilis (bacterium numbering 1.1413), Bifidobacterium (bacterium numbering 1.1852)
Embodiment 1:
A kind of production method of producing high DHA low cholesterol eggs nutritional agents: marine alga is fermented, obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents.
Fermentation is carried out in accordance with the following steps, obtains tunning;
(1) prepare shake-flask seed liquid: taking saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio is 3:1:1, under aseptic condition, starting strain is inoculated in fluid nutrient medium, cultivate 16h, obtain shake-flask seed liquid;
(2) prepare saccharomycete seed liquid: shake-flask seed liquid by volume, with 2% inoculum concentration, under aseptic condition, is inoculated in 50L seeding tank, cultivate 14h, fermentation obtains saccharomycete seed liquid;
(3) fermented and cultured: the saccharomycete seed liquid that step 3 is obtained, be by volume inoculated in 500L fermentation tank with 5%, between yeast phase, frequently add marine alga, the interpolation quality of marine alga is 10% of fermentation cumulative volume, cultivates 24h, stops tank tapping, obtains tunning.
The preferred chlorella of marine alga; Be 20 order powder, process pasteurization before fermentation.
In step (1), inoculum concentration is 4% of fluid nutrient medium volume, and shake-flask seed medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, and dipotassium hydrogen phosphate 0.3%, 20%NaOH solution regulates pH=7.5; Shake-flask seed condition of culture is preferably: 32 DEG C of temperature, and 120rpm, liquid amount is 140mL;
In step (2), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Saccharomycete seed liquid condition of culture is: 37 DEG C, and mixing speed 200rpm, liquid amount is 30L, throughput 2.0L/h;
In step (3), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Fermentation condition is: 32 DEG C of temperature, and mixing speed 100rpm, liquid amount is 300L, throughput 2.0L/h.
Described culture medium all passes through moist heat sterilization, preferably 121 DEG C of sterilising conditions, autoclaving 20-30min.
One is produced high DHA low cholesterol eggs nutritional agents by fermentation of seaweed product, fish oil, linseed oil composition; Described fermentation of seaweed product accounts for 10% of total nutritional agents weight, fish oil account for total nutritional agents weight 50%, linseed oil account for 40% of total nutritional agents weight, nutritional agents gross weight is 100 parts.
The high DHA low cholesterol eggs feed of a kind of production: it is characterized in that: described feed optimization formula is as follows: corn flour accounts for 60% of feed gross weight, dregs of beans accounts for 22% of feed gross weight, oyster shell whiting accounts for 10% of feed gross weight, calcium monohydrogen phosphate accounts for 2% of feed gross weight, vitamin accounts for 2% of feed gross weight, mineral matter accounts for 2% of feed gross weight, and nutritional agents accounts for 2% of feed gross weight.
Described Vitamin U is elected as: vitamin A, vitamin B2, cobalamin, vitamin D, vitamin E, folic acid; Described mineral matter is preferably: the compound of various trace elements, comprises calcium, iron, zinc, manganese, selenium etc.
Detect DHA content 420mg/100g in egg, cholesterol level DHA content 290mg/100g.
Embodiment 2:
A kind of production method of producing high DHA low cholesterol eggs nutritional agents: marine alga is fermented, obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents.
Fermentation is carried out in accordance with the following steps, obtains tunning;
(1) prepare shake-flask seed liquid: taking saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio is 3:1:1, under aseptic condition, starting strain is inoculated in fluid nutrient medium, cultivate 18h, obtain shake-flask seed liquid;
(2) prepare saccharomycete seed liquid: shake-flask seed liquid by volume, with 5% inoculum concentration, under aseptic condition, is inoculated in 50L seeding tank, cultivate 16h, fermentation obtains saccharomycete seed liquid;
(3) fermented and cultured: the saccharomycete seed liquid that step 3 is obtained, be by volume inoculated in 500L fermentation tank with 20%, between yeast phase, frequently add marine alga, the interpolation quality of marine alga is 20% of fermentation cumulative volume, cultivates 30h, stops tank tapping, obtains tunning.
The preferred sargassum thunbergii of marine alga; Be 60 order powder, process pasteurization before fermentation.
In step (1), inoculum concentration is 10% of fluid nutrient medium volume, and shake-flask seed medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, and dipotassium hydrogen phosphate 0.3%, 20%NaOH solution regulates pH=7.5; Shake-flask seed condition of culture is preferably: 37 DEG C of temperature, and 200rpm, liquid amount is 200mL;
In step (2), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Saccharomycete seed liquid condition of culture is: 37 DEG C, and mixing speed 250rpm, liquid amount is 50L, throughput 2.5L/h;
In step (3), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Fermentation condition is: 37 DEG C of temperature, and mixing speed 150rpm, liquid amount is 500L, throughput 3.0L/h.
Described culture medium all passes through moist heat sterilization, preferably 121 DEG C of sterilising conditions, autoclaving 20-30min.
One is produced high DHA low cholesterol eggs nutritional agents by fermentation of seaweed product, fish oil, linseed oil composition; Described fermentation of seaweed product accounts for 30% of total nutritional agents weight, fish oil account for total nutritional agents weight 50%, linseed oil account for 20% of total nutritional agents weight, nutritional agents gross weight is 100 parts.
The high DHA low cholesterol eggs feed of a kind of production: it is characterized in that: described feed optimization formula is as follows: corn flour accounts for 70% of feed gross weight, dregs of beans accounts for 20% of feed gross weight, oyster shell whiting accounts for 5% of feed gross weight, calcium monohydrogen phosphate accounts for 1.5% of feed gross weight, vitamin accounts for 1.5% of feed gross weight, mineral matter accounts for 1.5% of feed gross weight, and nutritional agents accounts for 0.5% of feed gross weight.
Described Vitamin U is elected as: vitamin A, vitamin B2, cobalamin, vitamin D, vitamin E, folic acid; Described mineral matter is preferably: the compound of various trace elements, comprises calcium, iron, zinc, manganese, selenium etc.
Detect DHA content 430mg/100g in egg, cholesterol level DHA content 220mg/100g.
Embodiment 3:
A kind of production method of producing high DHA low cholesterol eggs nutritional agents: marine alga is fermented, obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents.
Fermentation is carried out in accordance with the following steps, obtains tunning;
(1) prepare shake-flask seed liquid: taking saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio is 3:1:1, under aseptic condition, starting strain is inoculated in fluid nutrient medium, cultivate 17h, obtain shake-flask seed liquid;
(2) prepare saccharomycete seed liquid: shake-flask seed liquid by volume, with 4% inoculum concentration, under aseptic condition, is inoculated in 50L seeding tank, cultivate 15h, fermentation obtains saccharomycete seed liquid;
(3) fermented and cultured: the saccharomycete seed liquid that step 3 is obtained, be by volume inoculated in 500L fermentation tank with 10%, between yeast phase, frequently add marine alga, the interpolation quality of marine alga is 15% of fermentation cumulative volume, cultivates 25h, stops tank tapping, obtains tunning.
The preferred chrysophyceae of marine alga; Be 40 order powder, process pasteurization before fermentation.
In step (1), inoculum concentration is 5% of fluid nutrient medium volume, and shake-flask seed medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, and dipotassium hydrogen phosphate 0.3%, 20%NaOH solution regulates pH=7.5; Shake-flask seed condition of culture is preferably: 35 DEG C of temperature, and 150rpm, liquid amount is 180mL;
In step (2), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Saccharomycete seed liquid condition of culture is: 37 DEG C, and mixing speed 220rpm, liquid amount is 40L, throughput 2.2L/h;
In step (3), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Fermentation condition is: 35 DEG C of temperature, and mixing speed 120rpm, liquid amount is 400L, throughput 2.5L/h.
Described culture medium all passes through moist heat sterilization, preferably 121 DEG C of sterilising conditions, autoclaving 20-30min.
One is produced high DHA low cholesterol eggs nutritional agents by fermentation of seaweed product, fish oil, linseed oil composition; Described fermentation of seaweed product accounts for 30% of total nutritional agents weight, fish oil account for total nutritional agents weight 20%, linseed oil account for 50% of total nutritional agents weight, nutritional agents gross weight is 100 parts.
The high DHA low cholesterol eggs feed of a kind of production: it is characterized in that: described feed optimization formula is as follows: corn flour accounts for 60% of feed gross weight, dregs of beans accounts for 30% of feed gross weight, oyster shell whiting accounts for 6% of feed gross weight, calcium monohydrogen phosphate accounts for 1% of feed gross weight, vitamin accounts for 1% of feed gross weight, mineral matter accounts for 1% of feed gross weight, and nutritional agents accounts for 1% of feed gross weight.
Described Vitamin U is elected as: vitamin A, vitamin B2, cobalamin, vitamin D, vitamin E, folic acid; Described mineral matter is preferably: the compound of various trace elements, comprises calcium, iron, zinc, manganese, selenium etc.
Detect DHA content 440mg/100g in egg, cholesterol level DHA content 210mg/100g.
Embodiment 4:
A kind of production method of producing high DHA low cholesterol eggs nutritional agents: marine alga is fermented, obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents.
Fermentation is carried out in accordance with the following steps, obtains tunning;
(1) prepare shake-flask seed liquid: taking saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio is 3:1:1, under aseptic condition, starting strain is inoculated in fluid nutrient medium, cultivate 16.5h, obtain shake-flask seed liquid;
(2) prepare saccharomycete seed liquid: shake-flask seed liquid by volume, with 3% inoculum concentration, under aseptic condition, is inoculated in 50L seeding tank, cultivate 15.5h, fermentation obtains saccharomycete seed liquid;
(3) fermented and cultured: the saccharomycete seed liquid that step 3 is obtained, be by volume inoculated in 500L fermentation tank with 12%, between yeast phase, frequently add marine alga, the interpolation quality of marine alga is 18% of fermentation cumulative volume, cultivates 27h, stops tank tapping, obtains tunning.
Preferred chrysophyceae mixes with chlorella marine alga; Be 30 order powder, process pasteurization before fermentation.
In step (1), inoculum concentration is 5% of fluid nutrient medium volume, and shake-flask seed medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, and dipotassium hydrogen phosphate 0.3%, 20%NaOH solution regulates pH=7.5; Shake-flask seed condition of culture is preferably: 36 DEG C of temperature, and 145rpm, liquid amount is 160mL;
In step (2), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Saccharomycete seed liquid condition of culture is: 37 DEG C, and mixing speed 240rpm, liquid amount is 35L, throughput 2.4L/h;
In step (3), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%, manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Fermentation condition is: 36 DEG C of temperature, and mixing speed 115rpm, liquid amount is 350L, throughput 2.8L/h.
Described culture medium all passes through moist heat sterilization, preferably 121 DEG C of sterilising conditions, autoclaving 20-30min.
One is produced high DHA low cholesterol eggs nutritional agents by fermentation of seaweed product, fish oil, linseed oil composition; Described fermentation of seaweed product accounts for 20% of total nutritional agents weight, fish oil account for total nutritional agents weight 45%, linseed oil account for 35% of total nutritional agents weight, nutritional agents gross weight is 100 parts.
The high DHA low cholesterol eggs feed of a kind of production: it is characterized in that: described feed optimization formula is as follows: corn flour accounts for 66% of feed gross weight, dregs of beans accounts for 21% of feed gross weight, oyster shell whiting accounts for 8% of feed gross weight, calcium monohydrogen phosphate accounts for 1% of feed gross weight, vitamin accounts for 1% of feed gross weight, mineral matter accounts for 1% of feed gross weight, and nutritional agents accounts for 2% of feed gross weight.
Described Vitamin U is elected as: vitamin A, vitamin B2, cobalamin, vitamin D, vitamin E, folic acid; Described mineral matter is preferably: the compound of various trace elements, comprises calcium, iron, zinc, manganese, selenium etc.
Detect DHA content 445mg/100g in egg, cholesterol level DHA content 275mg/100g.
Claims (9)
1. a production method of producing high DHA low cholesterol eggs nutritional agents, is characterized in that: comprise following production stage: marine alga is fermented,Obtain fermentation of seaweed product, described fermentation of seaweed product is mixed with fish oil, linseed oil, make nutritional agents;
Described fermentation is carried out in accordance with the following steps, obtains tunning;
(1) prepare shake-flask seed liquid: taking saccharomycete, bacillus subtilis, Bifidobacterium as starting strain, inoculum concentration ratio is 3:1:1, aseptic conditionUnder, starting strain is inoculated in fluid nutrient medium, cultivate 16-18h, obtain shake-flask seed liquid;
(2) prepare saccharomycete seed liquid: shake-flask seed liquid by volume, with the inoculum concentration of 2-5%, under aseptic condition, is inoculated in 50L seeding tank, trainingSupport 14-16h, fermentation obtains saccharomycete seed liquid;
(3) fermented and cultured: the saccharomycete seed liquid that step 2 is obtained, be by volume inoculated in 500L fermentation tank with 5-20%, between yeast phase, frequently addEnter marine alga, the interpolation quality of marine alga is the 10-20% of fermentation cumulative volume, cultivates 24-30h, stops tank tapping, obtains tunning.
2. a kind of production method of producing high DHA low cholesterol eggs nutritional agents according to claim 1, is characterized in that: described marine alga is preferredBe rich in the algae of DHA; Described marine alga is 20-60 order powder, process pasteurization before fermentation.
3. a kind of production method of producing high DHA low cholesterol eggs nutritional agents according to claim 1, is characterized in that: described marine alga is preferredFor any one or a few in chlorella, sargassum thunbergii, Freshwater Chrysophytes.
4. a kind of production method of producing high DHA low cholesterol eggs nutritional agents according to claim 1, is characterized in that: in step (1),Inoculum concentration is the 4-10% of fluid nutrient medium volume, and shake-flask seed medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, phosphoric acidHydrogen dipotassium 0.3%, 20%NaOH solution regulates pH=7.5; Shake-flask seed condition of culture is preferably: temperature 32-37 DEG C, 120-200rpm, dress liquidAmount is 140-200mL;
In step (2), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%,Defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Saccharomycete seed liquid condition of culture is: 37 DEG C, and mixing speed 200-250rpm, dressLiquid measure is 30L-50L, throughput 2.0-2.5L/h;
In step (3), medium optimization formula is: glucose 0.5%, 20 order corn flour 1.0%, 20 order bean cake powder 3%, and dipotassium hydrogen phosphate 0.3%,Manganese sulfate 0.05%, defoamer 0.07%, 20%NaOH solution regulates pH=7.5; Fermentation condition is preferably: temperature 32-37 DEG C, mixing speed100-150rpm, liquid amount is 300-500L, throughput 2.0-3.0L/h.
5. according to a kind of production method of producing high DHA low cholesterol eggs nutritional agents described in claim 1-4 any one, it is characterized in that: instituteState culture medium and all pass through moist heat sterilization, preferably 121 DEG C of sterilising conditions, autoclaving 20-30min.
6. the alimentation of eggs agent that a kind of production method of producing high DHA low cholesterol eggs nutritional agents based on described in claim 1-5 any one obtains,It is characterized in that: described nutritional agents is by fermentation of seaweed product fish oil, linseed oil composition.
7. alimentation of eggs agent according to claim 6, is characterized in that: described fermentation of seaweed product accounts for the 10-30% of total nutritional agents weight, fish oilAccount for the 20-50% of total nutritional agents weight, the 20-50% that accounts for total nutritional agents weight of linseed oil, nutritional agents gross weight is 100 parts.
8. a birds, beasts and eggs feed that contains alimentation of eggs agent as claimed in claim 6, is characterized in that: described feed optimization formula is as follows: corn flour accounts forThe 60-70% of feed gross weight, dregs of beans accounts for the 20-30% of feed gross weight, and oyster shell whiting accounts for the 5-10% of feed gross weight, and it is total that calcium monohydrogen phosphate accounts for feedThe 1-2% of weight, vitamin accounts for the 1-2% of feed gross weight, and mineral matter accounts for the 1-2% of feed gross weight, and nutritional agents accounts for the 0.5-2% of feed gross weight.
9. a kind of high DHA low cholesterol eggs feed of producing according to claim 8, is characterized in that: the bird that described feed is preferably fed isAny one in chicken, duck, goose.
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| CN104381653A (en) * | 2014-11-12 | 2015-03-04 | 浙江省长兴艾格生物制品有限公司 | Chicken feed and chicken breeding method |
| CN104382085A (en) * | 2014-11-12 | 2015-03-04 | 浙江省长兴艾格生物制品有限公司 | High-phospholipid type DHA (docosahexenoic acid) and low-cholesterol yolk powder |
| CN105166519A (en) * | 2015-09-14 | 2015-12-23 | 南京林业大学 | Compound biological feed additive |
| CN105533251A (en) * | 2015-12-30 | 2016-05-04 | 天津市寿南山禽蛋营养研发有限公司 | Method and nutritional agent for producing poultry eggs rich in DHA and selenium |
| CN105494261A (en) * | 2015-12-30 | 2016-04-20 | 天津市寿南山禽蛋营养研发有限公司 | Method and nutrient for producing DHA (Docosahexaenoic Acid) and zinc-rich eggs |
| CN105533250A (en) * | 2015-12-30 | 2016-05-04 | 天津市寿南山禽蛋营养研发有限公司 | Method and nutritional agent for producing bird eggs rich in DHA and folic acid |
| CN105532574A (en) * | 2015-12-30 | 2016-05-04 | 天津市寿南山禽蛋营养研发有限公司 | Production method of eggs rich in DHA and iron elements and nutritional agent |
| CN106561972A (en) * | 2016-10-09 | 2017-04-19 | 青岛琅琊台集团股份有限公司 | Method for producing biological feed rich in nucleic acid, DHA and proteins |
| CN107455605B (en) * | 2017-09-21 | 2020-10-16 | 徐州正昌饲料有限公司 | Feed compound for reducing cholesterol content of egg yolk |
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| CN114885893A (en) * | 2022-05-06 | 2022-08-12 | 禄丰双丰良种猪有限公司 | Production method of three-high one-low green shell eggs |
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| CN101444256A (en) * | 2007-11-26 | 2009-06-03 | 张儒德 | Chicken feed additive for increasing the DHA content of eggs |
| CN102715382B (en) * | 2012-07-12 | 2013-05-22 | 湖南农业大学 | Feed regulating and controlling agent for producing eggs enriching dehydroacetic acid (DHA) and feed containing feed regulating and controlling agent |
| CN102907573B (en) * | 2012-08-01 | 2013-09-04 | 中国农业大学 | Feed additive for improving DHA conversion efficiency and content in poultry egg, and application thereof |
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