CN103664318A - High-quality hericium erinaceus culture medium - Google Patents
High-quality hericium erinaceus culture medium Download PDFInfo
- Publication number
- CN103664318A CN103664318A CN201210354216.9A CN201210354216A CN103664318A CN 103664318 A CN103664318 A CN 103664318A CN 201210354216 A CN201210354216 A CN 201210354216A CN 103664318 A CN103664318 A CN 103664318A
- Authority
- CN
- China
- Prior art keywords
- hericium erinaceus
- culture medium
- quality
- pers
- bull
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a high-quality hericium erinaceus culture medium and particularly relates to a formula and preparation method of a high-quality hericium erinaceus culture medium. The high-quality hericium erinaceus culture medium is prepared by using a special process, is a high-yield and disease-preventive culture medium special for culturing hericium erinaceus and is used for specification authentication of a high-quality hericium erinaceus strain, the enriched culture and strain passage of the hericium erinaceus and fermentation base culture of fungi. The culture medium is rich in nutrition, simple in preparation and strong in selectivity; compared with the traditional culture medium, the high-quality hericium erinaceus culture medium is high in output rate, little in pollution, satisfied in production and application effects and capable of greatly prolonging the life of a viable fungus; and the method is simple, short in time and high in accuracy.
Description
technical fieldthe present invention relates to a kind of high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium, particularly formula of high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium and preparation method thereof.Its main component is that Tryptones, multivalence peptone, yeast extract powder, beef extract powder, glucose, maltose, Pericarppium arachidis hypogaeae, sodium-chlor, vitamin complex, dipotassium hydrogen phosphate, wheat bran are prepared from; Hedgehog hydnum is antimycotic, antibacterium, antiviral ability are stronger.Therefore, fall ill in the course of cultivation less, also lighter.During Hericium erinaceus (Bull. Ex Fr.) Pers. produces, common a kind of phenomenon is sporophore atrophy, i.e. sporophore early growth is normal, and bacterium thorn was with regard to atrophy lodging, the shrinkage of mushroom body afterwards.This is non-cause of disease disease, is due to excess Temperature, dry air, and sporophore lack of water causes.The space, mushroom room of should spraying in time, improves indoor air relative humidity to 85%.Non-cause of disease disease be due to unfavorable envrionment conditions or inappropriate cultivation step caused.As too high or too low in culture material water content, pH is too small or excessive.Relative air humidity is excessive or too small, and light is crossed by force or excessively, density of carbon dioxide gas overrich, and the factors such as improper use such as agricultural chemicals, growth regulatory substance cause, this class disease is noncommunicable.There is relatively severe deformities mushroom in Hericium erinaceus (Bull. Ex Fr.) Pers., just belongs to this disease.During Hericium erinaceus (Bull. Ex Fr.) Pers. produces, common another kind of phenomenon is that after sporophore atrophy, browning is mouldy again soon, and this is infected by mould, is cause of disease disease.Should strengthen mushroom room and ventilate, reduce indoor humidity, and in affected part, smear 3% Eusol, or 1% lime solution, prevent that it from spreading.Cause of disease disease is owing to being subject to the parasitic disease causing of harmful microbe, being also infectious disease.Cause of disease disease has infectivity, so be also contagious disease.The biology that causes Hericium erinaceus (Bull. Ex Fr.) Pers. morbidity is referred to as pathogen.Pathogen mainly contains fungi, bacterium, virus etc.Cause of disease disease control, except carrying out in real earnest the measures of integrated control noted earlier, key is the preparation of substratum and the quality of formula.High-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium of the present invention is to cultivate high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi high yield special culture media.
background technologyfor a long time, people mention in the same breath it and bird's nest.Hericium erinaceus (Bull. Ex Fr.) Pers. has good tonic effect, can treat the diseases such as maldigestion, stomach ulcer, duodenal ulcer, neurasthenia, among the peoplely has saying of " eating hedgehog hydnum more, recover one's youthful vigour ".The traditional Chinese medical science thinks that Hericium erinaceus (Bull. Ex Fr.) Pers. property is flat, taste is sweet.Profit the five internal organs are aid digestion; There is stomach invigorating, qi-restoratives, anticancer, the effect Hericium erinaceus (Bull. Ex Fr.) Pers. of kidney-nourishing essence cures mainly the illnesss such as anorexia and loose stool, gastric and duodenal ulcer, neurasthenia, esophagus cancer, cancer of the stomach, dizzy, impotence.Elderly and infirm eats Hericium erinaceus (Bull. Ex Fr.) Pers., has the effect of nourishing and fit keeping function.. Hericium erinaceus (Bull. Ex Fr.) Pers. is a kind of good food of a kind of high protein, lower fat, rich in mineral substances and VITAMIN; Hericium erinaceus (Bull. Ex Fr.) Pers., containing unsaturated fatty acids, can reduce blood cholesterol and content of triglyceride, regulates blood fat, is beneficial to blood circulation, is the ultimate food of cardiovascular patient; The polysaccharide body that Hericium erinaceus (Bull. Ex Fr.) Pers. contains, polypeptide class and fatty substance, in energy anticancer, genetic material is synthetic, thus prevention and treatment alimentary tract cancer and other malignant tumours; In Hericium erinaceus (Bull. Ex Fr.) Pers., contain multiple amino acids and abundant polysaccharide body, can be aid digestion, the curative effect of the digestive tract diseases such as gastritis, cancer of the stomach, esophagus cancer, stomach ulcer, duodenal ulcer is attracted people's attention; Hericium erinaceus (Bull. Ex Fr.) Pers. has the function that improves body immunity, delaying sanility.Take Hericium erinaceus (Bull. Ex Fr.) Pers. for the slight insomnia good assisting therapy of can yet be regarded as.In addition, insomniac should cultivate good living habit, as take a walk after supper, usually does more physical exercises more, also should adjust work habit training interest, in the middle of devoting oneself to work with positive phychology.These recoveries for symptom all have good help.The object of the present invention is to provide high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium, for the standard of high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi strain, identify, and Hericium erinaceus (Bull. Ex Fr.) Pers. fungi increases, and bacterium is cultivated and bacterial classification goes down to posterity, cultivate on the fermentation of fungi mushroom basis.This culture medium culturing base is nutritious, preparation simple, selectivity is strong, and with traditional substratum comparison, output capacity is high, pollute little, production application good results. greatly improve the viable bacteria life-span, method is simple, the time is short, accuracy is high.
summary of the inventionmain points of the present invention are to select suitable component, and carry out rational mixture, through heating, dissolve, the technique such as mixing, cooling, packing, high-temperature sterilization forms high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium.
Culture medium prescription that the present invention selects following (gram/L) Tryptones (or domestic trypticase) 8.0-10.0; Multivalence peptone 8.0-10.0; Extractum carnis (powder) 3.0-4.0; Sodium-chlor 4.0-5.0; Glucose 1.0-2.0; Maltose 1.0-2.0; Wheat bran 20.0-30.0; Pericarppium arachidis hypogaeae 40.0-50.0; Yeast powder 3.0-4.0; Vitamin complex 1.0-2.0; Dipotassium hydrogen phosphate 1.0-2.0; Distilled water adds to 1000 mL.
High-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium, starting material are easy to get, easy to make, basic ingredient be take Mycoplasma Broth Base as main, adjust the component proportions of multivalence peptone, add nutritious beef extract powder, yeast extract powder, yeast extract powder is mainly the breeding of high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungal growth solubility nitrogenous substances, nucleic acid degradation product, VITAMIN and inorganic salts etc. is provided, to supplement the insufficient section of peptone nutritive ingredient.
Phosphoric acid salt is the good phosphorus source of bacterium and has shock absorption concurrently.The effect of sodium-chlor is to regulate osmotic pressure, especially in preparation, during containing the substratum of high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi, needs isotonic solution, and it also can form in thalline the activator as enzyme.
The preparation method of product of the present invention is:
1), Tryptones, multivalence peptone, yeast extract powder, beef extract powder, glucose, maltose, sodium-chlor, vitamin complex, dipotassium hydrogen phosphate Hybrid Heating are dissolved to correction pH7.4;
2), stir in wheat bran, Pericarppium arachidis hypogaeae, packing, through 121 ℃ of 15min autoclavings.
The present invention has the following advantages: the substratum of (1) high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi, and nutritious, preparation is simple; (2) selectivity is strong, output capacity is high, it is little to pollute, and effect is satisfied; (3) cultural method is simple, the time is short, accuracy is high.(4) highly sensitive, high specificity, good stability.
embodimentbelow in conjunction with embodiment, the present invention is described in further detail:
According to following table institute column data (gram/L) and described step thereof, be configured:
Formula one:
Tryptones (or domestic trypticase) 8.0-10.0; Multivalence peptone 8.0-10.0; Extractum carnis (powder) 3.0-4.0; Sodium-chlor 4.0-5.0; Glucose 1.0-2.0; Maltose 1.0-2.0; Wheat bran 20.0-30.0; Pericarppium arachidis hypogaeae 40.0-50.0; Yeast powder 3.0-4.0; Vitamin complex 1.0-2.0; Dipotassium hydrogen phosphate 1.0-2.0; Distilled water adds to 1000 mL
____________________________________________________________________
Formula two:
Tryptones (or domestic trypticase) 9.0-10.0; Multivalence peptone 9.0-10.0; Extractum carnis (powder) 3.5-4.0; Sodium-chlor 4.5-5.0; Glucose 1.5-2.0; Maltose 1.5-2.0; Wheat bran 25.0-30.0; Pericarppium arachidis hypogaeae 45.0-50.0; Yeast powder 3.5-4.0; Vitamin complex 1.5-2.0; Dipotassium hydrogen phosphate 1.5-2.0; Distilled water adds to 1000 mL
____________________________________________________________________
Formula three:
Tryptones (or domestic trypticase) 8.0-9.0; Multivalence peptone 8.0-9.0; Extractum carnis (powder) 3.0-3.8; Sodium-chlor 4.0-4.6; Glucose 1.0-1.6; Maltose 1.0-1.8; Wheat bran 26.0-28.0; Pericarppium arachidis hypogaeae 42.0-49.0; Yeast powder 3.2-3.8; Vitamin complex 1.2-1.7; Dipotassium hydrogen phosphate 1.1-1.8; Distilled water adds to 1000 mL.
Claims (2)
1. the present invention relates to a kind of high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium, it is characterized in that having following formula (gram/L) Tryptones (or domestic trypticase) 8.0-10.0; Multivalence peptone 8.0-10.0; Extractum carnis (powder) 3.0-4.0; Sodium-chlor 4.0-5.0; Glucose 1.0-2.0; Maltose 1.0-2.0; Wheat bran 20.0-30.0; Pericarppium arachidis hypogaeae 40.0-50.0; Yeast powder 3.0-4.0; Vitamin complex 1.0-2.0; Dipotassium hydrogen phosphate 1.0-2.0; Distilled water adds to 1000 mL.
2. a preparation method for high-quality Hericium erinaceus (Bull. Ex Fr.) Pers. fungi culture medium described in claim 1, is characterized in that having following step:
1), Tryptones, multivalence peptone, yeast extract powder, beef extract powder, glucose, maltose, sodium-chlor, vitamin complex, dipotassium hydrogen phosphate Hybrid Heating are dissolved to correction pH7.4;
2), stir in wheat bran, Pericarppium arachidis hypogaeae, packing, through 121 ℃ of 15min autoclavings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210354216.9A CN103664318A (en) | 2012-09-21 | 2012-09-21 | High-quality hericium erinaceus culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210354216.9A CN103664318A (en) | 2012-09-21 | 2012-09-21 | High-quality hericium erinaceus culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103664318A true CN103664318A (en) | 2014-03-26 |
Family
ID=50303217
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210354216.9A Pending CN103664318A (en) | 2012-09-21 | 2012-09-21 | High-quality hericium erinaceus culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103664318A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017117701A1 (en) * | 2016-01-04 | 2017-07-13 | 葡萄王生技股份有限公司 | Active substance for preventing degeneration of hearing, composition comprising same, and preparation method thereof |
| CN108610167A (en) * | 2016-12-19 | 2018-10-02 | 谢善慈 | The method for improving the edible mushroom cultivation nutrition element and application of lignin conversion rate the nutrient culturing edible fungus |
| CN111727810A (en) * | 2020-06-29 | 2020-10-02 | 新疆农业科学院植物保护研究所 | Special culture medium and culture method for wild petiole nail ash wrapped strain |
-
2012
- 2012-09-21 CN CN201210354216.9A patent/CN103664318A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017117701A1 (en) * | 2016-01-04 | 2017-07-13 | 葡萄王生技股份有限公司 | Active substance for preventing degeneration of hearing, composition comprising same, and preparation method thereof |
| US10405504B2 (en) | 2016-01-04 | 2019-09-10 | Grape King Bio Ltd. | Active substances for preventing hearing deterioration, the composition containing the active substances, and the preparation method thereof |
| CN108610167A (en) * | 2016-12-19 | 2018-10-02 | 谢善慈 | The method for improving the edible mushroom cultivation nutrition element and application of lignin conversion rate the nutrient culturing edible fungus |
| CN108610167B (en) * | 2016-12-19 | 2021-04-02 | 南平市农康生物技术有限公司 | Edible fungus cultivation nutrient for improving lignin conversion rate and method for cultivating edible fungus by using nutrient |
| CN111727810A (en) * | 2020-06-29 | 2020-10-02 | 新疆农业科学院植物保护研究所 | Special culture medium and culture method for wild petiole nail ash wrapped strain |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5517215B2 (en) | Fermentation and culture method, plant fermented extract, plant fermented extract powder and blended plant fermented extract | |
| CN101760478B (en) | Preparation method of radix puerariae red yeast rice | |
| CN102366023B (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN102366025B (en) | Method for preparing grass carp feed additive containing traditional Chinese medicine active probiotics | |
| CN108740145B (en) | Hangover alleviating black tea and preparation method thereof | |
| CN107197966A (en) | A kind of method of microorganism ferment making GABA tea | |
| CN105936606A (en) | Functional edible fungus culture substrate and method for culturing edible fungi by using same | |
| CN105249100A (en) | Production method of fermented fruit and vegetable juice and Chinese rice wine composited function beverage | |
| CN103141702A (en) | Chinese herb extract feed additive capable of effectively preventing and treating porcine epizootic diarrhea | |
| CN102726665B (en) | Method for producing corn flour by the solid state fermentation of maize pulp by using edible fungi | |
| CN104206923A (en) | Feed for chicken breeding | |
| CN103451144B (en) | Microbial growth promoters, Preparation Method And The Use | |
| CN103664318A (en) | High-quality hericium erinaceus culture medium | |
| CN108497118B (en) | Alcohol-dispelling health tea and preparation method thereof | |
| CN104718990B (en) | The production method of a kind of composite fungi feed production and Edible Fungi unification | |
| CN102334679A (en) | Method for preparing buckwheat pollen fermented health-care food | |
| CN105367303A (en) | Culture medium for culturing ganoderma lucidum | |
| CN104304561A (en) | Phellinus mycelium and tea fungus beverage | |
| CN102367417B (en) | Traditional Chinese medicine probiotics liquid medium used for fish feed, culturing method and feed additive | |
| CN103371367A (en) | Food for acquired immune deficiency syndrome nutritional therapy | |
| CN1772007A (en) | Fermented aweto hypha tablet | |
| CN107997125A (en) | Edible mushroom mycelium and or edible mushroom mycelium powder manufacture craft and products thereof | |
| CN107484547A (en) | A kind of method that medium culture Cordyceps militaris is made using blueberry leaf as major ingredient | |
| CN105347932A (en) | Hericium erinaceus culture medium | |
| CN107916208A (en) | A kind of lentinan zymotic fluid and its process for solid state fermentation for improving immunity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140326 |