CN103656635B - A kind of preparation method of Groton bug anaphylactogen preparation - Google Patents
A kind of preparation method of Groton bug anaphylactogen preparation Download PDFInfo
- Publication number
- CN103656635B CN103656635B CN201310694219.1A CN201310694219A CN103656635B CN 103656635 B CN103656635 B CN 103656635B CN 201310694219 A CN201310694219 A CN 201310694219A CN 103656635 B CN103656635 B CN 103656635B
- Authority
- CN
- China
- Prior art keywords
- groton bug
- anaphylactogen
- preparation
- powder
- groton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 41
- 239000013566 allergen Substances 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 25
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000284 extract Substances 0.000 claims abstract description 12
- 239000000287 crude extract Substances 0.000 claims abstract description 8
- 230000008014 freezing Effects 0.000 claims abstract description 7
- 238000007710 freezing Methods 0.000 claims abstract description 7
- 241000238631 Hexapoda Species 0.000 claims abstract description 6
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 6
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 6
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 6
- 230000009849 deactivation Effects 0.000 claims abstract description 6
- 238000005238 degreasing Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 6
- 239000006228 supernatant Substances 0.000 claims abstract description 6
- 238000012797 qualification Methods 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 108010011172 allergen Bla g 2 Proteins 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 6
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000007654 immersion Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 241001674044 Blattodea Species 0.000 description 5
- 208000006673 asthma Diseases 0.000 description 4
- 239000012925 reference material Substances 0.000 description 2
- 235000018185 Betula X alpestris Nutrition 0.000 description 1
- 235000018212 Betula X uliginosa Nutrition 0.000 description 1
- 208000035533 House dust allergy Diseases 0.000 description 1
- 206010039085 Rhinitis allergic Diseases 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 201000010105 allergic rhinitis Diseases 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
This statement provides a kind of preparation method of Groton bug anaphylactogen preparation, comprises the steps: that (1) is by Groton bug deactivation; (2) for subsequent use with ethanol cleaning, immersion, air-dry being placed on-80 DEG C Refrigerator store; (3) get appropriate freezing Groton bug grind into powder in liquid nitrogen; (4) freezing after the degreasing of use acetone, be lyophilized into subsequently powder; (5) take Groton bug powder and add extract, fully mix absorption supernatant on vortex oscillator, (6) identify content and the ratio of anaphylactogen in Groton bug anaphylactogen crude extract with the specific antibody of corresponding anti-allergen; (7) recombinant protein that adds saccharomycete or insect to express, making it reach with five kinds of main allergen content of Groton bug self and the uniformity of ratio is 65%～75%, obtains anaphylactogen preparation of the present invention. The Groton bug anaphylactogen crude extract that the inventive method is extracted can farthest obtain Groton bug main allergen albumen, extract favorable repeatability, and error is less than 30%.
Description
Technical field
The present invention relates to biological pharmacy technical field, be specially a kind of preparation side of Groton bug anaphylactogen preparationMethod.
Background technology
Anaphylactia is one of mankind's major disease. Its incidence of disease estimates to account for 22% of world population at present,And just increasing to be greater than every year 1% speed, and rise the most obvious with the incidence of disease of child patient. Its dangerEvil property can not be weighed with its death rate simply, and will consider that the high outbreak frequency of disease causes to patientFinancial burden. Because anaphylactia mostly occurs young crowd, it on human society and economic impact justMore serious. China is in industrialization transformation process, and its incidence of disease significantly increases, and has had a strong impact on China and has got involvedThe quality of life of population, operating efficiency, increased the weight of patient's financial burden. Only Europe was used for the treatment of every yearQuick patient's expense has exceeded 50,000,000,000 Euros, and continues with 3 times of speedups to Other diseases in development.The cost of asthma and allergic rhinitis is often only respectively up to 64 and 6,000,000,000 dollars by the U.S.. Still there is number in the whole nationThere are potential diagnosis and Treatment need in hundred million people. Therefore, active support China anaphylactogen in-vivo diagnostic andThe research and development for the treatment of preparation, can effectively prevent that the medical expense infiltration of China being caused because of external product from going up, and subtractsFew national medical expenditure and people burden.
Anaphylactogen standardization is a global problem, and China widens gradually in this field and American-European countries's gap.For example, the U.S. effectively manages anaphylactogen preparation, has set up specially FDA/CBER/LIB laboratory, is intended to buildBiological value calibration method, the setting of vertical anaphylactogen reference material, autopath's reference serum pond, standard are effectiveReasonably quality clearance standard. 1981, on American market, there is more than five thousand nonstandardized technique anaphylactogen productSell, wherein the overwhelming majority does not all have security and validation verification. And now, FDA is through anaphylactogen for many yearsStandardization System specification, only 19 kinds of the standardization anaphylactogens of official approval. Germany institute of biological products-PEIResearch institute (PaulEhrlichInstitute, PEI) has carried out same strategy, forces to carry out in GermanyStandardization anaphylactogen, strengthens supervision, has closed tens of anaphylactogen manufacturing enterprises. European Union is by continuous threeFramework plan of science and technology is subsidized, and has set up the main allergic protein restructuring reference material rBetv1 of birch and standardELISA calibration method, prepares to write European Pharmacopoeia. We utilize new extraction and recombinant technique in this project,Prepare second generation standardization anaphylactogen preparation.
As far back as the sixties in last century, just there is cockroach to cause the report of asthma. But until in recent years,, cockroach is being roaredThe effect in morbidity of breathing heavily just receives people's concern gradually. Data shows, cockroach allergy in U.S.'s asthmatic patientIncidence reach 27.5～42%, Europe is 3.9～24.5%, Africa is 30～44.6%, in China,In the big city such as Beijing, Shanghai, the irritated rate of the cockroach of children asthma is also up to 27.8～43.8%. AsthmaThe continuous rising of the incidence of disease, relevant with cockroach pollution aggravation to a great extent, cockroach becomes and is only second toThe second largest indoor anaphylactogen of dirt mite. At present common Groton bug anaphylactogen is out identified, makes theTwo generations standardization Groton bug anaphylactogen preparation be prepared with foundation. But, up to the present, there is no public affairsThe Groton bug preparation technology of exploitation table, and Groton bug anaphylactogen preparation has larger clinical demand, withThis our company develops second generation standardization Groton bug anaphylactogen preparation.
Summary of the invention
Technical problem solved by the invention is to provide a kind of preparation method of Groton bug anaphylactogen preparation,To solve the problem in above-mentioned background technology.
Technical problem solved by the invention realizes by the following technical solutions: a kind of Groton bug anaphylactogenThe preparation method of preparation, comprises the steps:
(1) by Groton bug deactivation in-80 DEG C of refrigerators;
(2) clean, soak 15 minutes with 75% ethanol, air-dry being placed on-80 DEG C Refrigerator store is for subsequent use;
(3) get appropriate freezing Groton bug grind into powder in liquid nitrogen;
(4) use after acetone degreasing 2 times, be placed in-80 DEG C of refrigerator and cooled and freeze, subsequently in vacuum freeze dryingIn machine, be lyophilized into powder;
(5) take Groton bug powder, add corresponding according to the ratio of Groton bug powder 1g extract 1LExtract, on vortex oscillator, fully mix, put extracting on 4 DEG C of shaking tables, be 12000g at rotating speed afterwards,Centrifugal 30min under 4 DEG C of conditions, draws supernatant, is Groton bug anaphylactogen crude extract;
(6) with anti-Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, BlagtrypsinAnd main irritated in the specific antibody of a kind of less important allergen Bla g 5 qualification Groton bug anaphylactogen crude extractFormer Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin and a kind of less important mistakeThe content of quick former Blag5 and ratio;
(7) recombinant protein that adds saccharomycete or insect to express according to qualification result in step (6), makesIts reach with five kinds of main allergen Blag2 of Groton bug self or Blag9, Blag6.01, Blag7,BlagGSTD1, Blagtrypsin, a kind of less important allergen Bla g 5 content and ratio are basically identical, obtainAnaphylactogen preparation of the present invention.
It is 0.5～4h that described Groton bug powder adds extraction time on the rearmounted 4 DEG C of shaking tables of extract.
Described extract is high phosphatizing phthalate buffer (PBS), and filling a prescription is: NaCl:35.1g～70.2g,KCl：0.2g、Na2HPO4：1.42g、KH2PO4: 0.27g, adds 800mlddH2O adjusting pH value 5.0～9.0, be settled to 1L.
Five kinds of main allergen content of described Groton bug self and the uniformity of ratio are 65%～75%.
Described Groton bug, for pure lines, is multiplied into by a pair of parents.
Compared with prior art, the present invention has following beneficial effect: the Groton bug mistake that the inventive method is extractedQuick former crude extract can farthest obtain Groton bug main allergen albumen, extract favorable repeatability,Error is less than 30%.
Brief description of the drawings
Fig. 1 is 1 hour result of different N aCl concentration PBS solution extracting Groton bug in the present invention.
Detailed description of the invention
Reach object and merit in order to make technological means of the present invention, creation characteristic, workflow, using methodEffect is easy to understand, and below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is enteredRow is described clearly and completely, and obviously, described embodiment is only the present invention's part embodiment, andNot whole embodiment. Based on the embodiment in the present invention, those of ordinary skill in the art are not makingThe every other embodiment obtaining under creative work prerequisite, belongs to the scope of protection of the invention.
Embodiment 1
A preparation method for Groton bug anaphylactogen preparation, comprises the steps:
(1) by Groton bug deactivation in-80 DEG C of refrigerators;
(2) clean, soak 15 minutes with 75% ethanol, air-dry being placed on-80 DEG C Refrigerator store is for subsequent use;
(3) get appropriate freezing Groton bug grind into powder in liquid nitrogen;
(4) use after acetone degreasing 2 times, be placed in-80 DEG C of refrigerator and cooled and freeze, subsequently in vacuum freeze dryingIn machine, be lyophilized into powder;
(5) take Groton bug powder, add corresponding according to the ratio of Groton bug powder 1g extract 1LExtract, on vortex oscillator, fully mix, put extracting 1h on 4 DEG C of shaking tables, at rotating speed be afterwards12000g, centrifugal 30min under 4 DEG C of conditions, draws supernatant, is Groton bug anaphylactogen crude extract;
(6) with anti-Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, BlagtrypsinAnd main in the specific antibody of a kind of less important allergen Bla g 5 anaphylactogen qualification Groton bug anaphylactogen crude extractWant allergen Bla g 2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin and a kindThe content of less important allergen Bla g 5 and ratio;
(7) recombinant protein that adds saccharomycete or insect to express according to qualification result in step (6), makesMain allergen Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin, 1The uniformity of planting less important allergen Bla g 5 content and ratio is 68%, obtains anaphylactogen preparation of the present invention.
Embodiment 2
A preparation method for Groton bug anaphylactogen preparation, comprises the steps:
(1) by Groton bug deactivation in-80 DEG C of refrigerators;
(2) clean, soak 15 minutes with 75% ethanol, air-dry being placed on-80 DEG C Refrigerator store is for subsequent use;
(3) get appropriate freezing Groton bug grind into powder in liquid nitrogen;
(4) use after acetone degreasing 2 times, be placed in-80 DEG C of refrigerator and cooled and freeze, subsequently in vacuum freeze dryingIn machine, be lyophilized into powder;
(5) take Groton bug powder, add corresponding according to the ratio of Groton bug powder 1g extract 1LExtract, on vortex oscillator, fully mix, put extracting 1.5h on 4 DEG C of shaking tables, at rotating speed be afterwards12000g, centrifugal 30min under 4 DEG C of conditions, draws supernatant, is Groton bug anaphylactogen crude extract;
(6) with anti-Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, BlagtrypsinAnd main in the specific antibody of a kind of less important allergen Bla g 5 anaphylactogen qualification Groton bug anaphylactogen crude extractWant allergen Bla g 2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin and a kindThe content of less important allergen Bla g 5 and ratio;
(7) recombinant protein that adds saccharomycete or insect to express according to qualification result in step (6), makesMain allergen Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin, 1The uniformity of planting less important allergen Bla g 5 content and ratio is 70%, obtains anaphylactogen preparation of the present invention.
Embodiment 3
A preparation method for Groton bug anaphylactogen preparation, comprises the steps:
(1) by Groton bug deactivation in-80 DEG C of refrigerators;
(2) clean, soak 15 minutes with 75% ethanol, air-dry being placed on-80 DEG C Refrigerator store is for subsequent use;
(3) get appropriate freezing Groton bug grind into powder in liquid nitrogen;
(4) use after acetone degreasing 2 times, be placed in-80 DEG C of refrigerator and cooled and freeze, subsequently in vacuum freeze dryingIn machine, be lyophilized into powder;
(5) take Groton bug powder, add corresponding according to the ratio of Groton bug powder 1g extract 1LExtract, on vortex oscillator, fully mix, put extracting 2h on 4 DEG C of shaking tables, at rotating speed be afterwards12000g, centrifugal 30min under 4 DEG C of conditions, draws supernatant, is Groton bug anaphylactogen crude extract;
(6) with anti-Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, BlagtrypsinAnd main in the specific antibody of a kind of less important allergen Bla g 5 anaphylactogen qualification Groton bug anaphylactogen crude extractWant allergen Bla g 2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin and a kindThe content of less important allergen Bla g 5 and ratio;
(7) recombinant protein that adds saccharomycete or insect to express according to qualification result in step (6), makesMain allergen Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin, 1The uniformity of planting less important allergen Bla g 5 content and ratio is 72%, obtains anaphylactogen preparation of the present invention.
More than show and described general principle of the present invention, principal character and advantage of the present invention. The industryTechnical staff should understand, the present invention is not restricted to the described embodiments, in above-described embodiment and descriptionThat describes just illustrates principle of the present invention, without departing from the spirit and scope of the present invention, and the present inventionAlso have various changes and modifications, these changes and improvements all fall in the claimed scope of the invention. ThisThe claimed scope of invention is defined by appending claims and equivalent thereof.
Claims (5)
1. a preparation method for Groton bug anaphylactogen preparation, is characterized in that: comprise the steps:
(1) by Groton bug deactivation in-80 DEG C of refrigerators;
(2) clean, soak 15 minutes with 75% ethanol, air-dry being placed on-80 DEG C Refrigerator store is for subsequent use;
(3) get appropriate freezing Groton bug grind into powder in liquid nitrogen;
(4) use after acetone degreasing 2 times, be placed in-80 DEG C of refrigerator and cooled and freeze, subsequently in vacuum freeze dryingIn machine, be lyophilized into powder;
(5) take Groton bug powder, add corresponding according to the ratio of Groton bug powder 1g extract 1LExtract, on vortex oscillator, fully mix, put extracting on 4 DEG C of shaking tables, be 12000g at rotating speed afterwards,Centrifugal 30min under 4 DEG C of conditions, draws supernatant, is Groton bug anaphylactogen crude extract;
(6) with anti-Blag2 or Blag9, Blag6.01, Blag7, BlagGSTD1, BlagtrypsinAnd main in the specific antibody of a kind of less important allergen Bla g 5 anaphylactogen qualification Groton bug anaphylactogen crude extractWant allergen Bla g 2 or Blag9, Blag6.01, Blag7, BlagGSTD1, Blagtrypsin and a kindThe content of less important allergen Bla g 5 and ratio;
(7) recombinant protein that adds saccharomycete or insect to express according to qualification result in step (6), makesIts reach with five kinds of main allergen Blag2 of Groton bug self or Blag9, Blag6.01, Blag7,BlagGSTD1, Blagtrypsin, the uniformity of a kind of less important allergen Bla g 5 content and ratio is 65%～75%, obtain anaphylactogen preparation of the present invention.
2. the preparation method of a kind of Groton bug anaphylactogen preparation according to claim 1, its feature existsIn: it is 0.5～4h that described Groton bug powder adds extraction time on the rearmounted 4 DEG C of shaking tables of extract.
3. the preparation method of a kind of Groton bug anaphylactogen preparation according to claim 1, its feature existsIn: described extract is high phosphatizing phthalate buffer (PBS), and filling a prescription is: NaCl:35.1g～70.2g,KCl：0.2g、Na2HPO4：1.42g、KH2PO4: 0.27g, adds 800mlddH2O adjusting pH value 5.0～9.0, be settled to 1L.
4. the preparation method of a kind of Groton bug anaphylactogen preparation according to claim 1, its feature existsBe 65%～75% in five kinds of main allergen content of: described Groton bug self and the uniformity of ratio.
5. the preparation method of a kind of Groton bug anaphylactogen preparation according to claim 1, its feature existsIn: described Groton bug, for pure lines, is multiplied into by a pair of parents.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310694219.1A CN103656635B (en) | 2013-12-05 | 2013-12-05 | A kind of preparation method of Groton bug anaphylactogen preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310694219.1A CN103656635B (en) | 2013-12-05 | 2013-12-05 | A kind of preparation method of Groton bug anaphylactogen preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103656635A CN103656635A (en) | 2014-03-26 |
| CN103656635B true CN103656635B (en) | 2016-05-18 |
Family
ID=50296192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310694219.1A Active CN103656635B (en) | 2013-12-05 | 2013-12-05 | A kind of preparation method of Groton bug anaphylactogen preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103656635B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102227214A (en) * | 2008-11-28 | 2011-10-26 | 切尔卡西亚有限公司 | Compositions with reduced dimer formation |
-
2013
- 2013-12-05 CN CN201310694219.1A patent/CN103656635B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102227214A (en) * | 2008-11-28 | 2011-10-26 | 切尔卡西亚有限公司 | Compositions with reduced dimer formation |
Non-Patent Citations (2)
| Title |
|---|
| 5种德国小镰全虫蛋自提取方法的效果比较;黄艳红等;《中国媒介生物学及控制杂质》;20120630;第23卷(第3期);第209-210页 * |
| 安徽省3种常见蜚蝙的可溶性蛋自质组成分析;姚湧等;《安徽医科人学学报》;20010430;第36卷(第2期);第114-115页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103656635A (en) | 2014-03-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103405699B (en) | Beverage for preventing and treating respiratory diseases caused by haze and preparation method thereof | |
| CN104140953A (en) | Breast cancer multidrug-resistant cell strain constructed by virtue of epirubicin induction as well as construction method and application thereof | |
| CN115869345B (en) | Shennong chrysanthemum flavonoid compound and preparation method and application thereof | |
| CN104000197A (en) | Health care product or medicine composition capable of relieving fatigue and preparation method and purpose thereof | |
| CN104017100A (en) | Qamgur polysaccharide extract and preparation method | |
| CN103656635B (en) | A kind of preparation method of Groton bug anaphylactogen preparation | |
| CN103920147B (en) | A kind of preparation method of American cockroach allergen preparation | |
| CN108434363A (en) | A kind of Chinese medicine composition and preparation method thereof for anti-trioxypurine | |
| CN104962515A (en) | Application of rhizome panacis majoris saponin inducing stem cells differentiating hepatic cells and hepatosis curing medicine | |
| Pawan et al. | Study on harmful effects of opium on liver and lungs in chronic opium addicts of western Rajasthan | |
| CN104161850B (en) | The preparation method of a kind of Chinese medicine extract and the Chinese medicine extract of preparation thereof and purposes | |
| CN104098491B (en) | A kind of mucolyticum acid compound and the suction acetylcysteine solution containing this compound | |
| CN103932184B (en) | A kind of Chinese medicine composition | |
| CN103610048B (en) | To chemical damage health food having assistant protection function and preparation method thereof | |
| CN107126416A (en) | The preparation method of salbutamol sulfate aerosol inhalation solution | |
| CN102283859A (en) | Application of dicliptera chinensis polysaccharide | |
| CN104436172B (en) | A kind of preparation method of new animal model in asthma | |
| CN1919875A (en) | Cryptoporus volvatus polysaccharide, preparation and application thereof | |
| Rahman et al. | Clinical presentation of nasopharyngeal carcinoma in West Sumatra, Indonesia | |
| CN102258504A (en) | Compound salbutamol sulfate and ambroxol hydrochloride inhalation solution | |
| Najafi et al. | Trend in lung cancer incidence in Kermanshah province-Iran, 1997-2007 | |
| CN102058779A (en) | Traditional Chinese medicine decoction for treating asthma | |
| CN102205045A (en) | Compound pharmaceutical composition for treating upper airway cough syndrome and preparation method as well as application thereof | |
| CN103784712A (en) | Health care product capable of repairing lung injuries caused by environmental pollution and preparation method of health care product | |
| CN100387280C (en) | Phlegm transforming Chinese medicinal formulation for children and its preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160622 Address after: 121000 Jinzhou city of Liaoning Province Taihe District South West Ling in a beautiful day. D 35-97 Patentee after: He Shaoheng Address before: 232000 No. ten, No. seven, weft Road, Huainan hi tech Industrial Development Zone, Anhui, China Patentee before: ANHUI RAIN MIX BIOTECHNOLOGY CO., LTD. |