CN103655940B - A kind of Chinese medicine compound treating menopathy - Google Patents
A kind of Chinese medicine compound treating menopathy Download PDFInfo
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- CN103655940B CN103655940B CN201310656429.1A CN201310656429A CN103655940B CN 103655940 B CN103655940 B CN 103655940B CN 201310656429 A CN201310656429 A CN 201310656429A CN 103655940 B CN103655940 B CN 103655940B
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Abstract
The invention discloses a kind of Chinese medicine compound treating menopathy, this compound recipe is made up of Radix Bupleuri, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Fructus Viticis, Folium Ginkgo, Cortex Magnoliae Officinalis, Rhizoma Corydalis, Radix Glycyrrhizae eight taste medical material, after selection, clean system, Radix Bupleuri, Radix Angelicae Sinensis, liquorice beverage carry, the Radix Paeoniae Alba, Fructus Viticis, Folium Ginkgo, Cortex Magnoliae Officinalis, Rhizoma Corydalis alcohol extraction, add appropriate amount of auxiliary materials and make peroral dosage form after concentration.For treating women because of the mental symptom such as pain symptom and depressed emotion, fidgety irritability, insomnia and dreamful sleep such as headache produced by dysmenorrhea, premenstrual syndrome, menopausal syndrome, distending pain of the breast, pelvic pain, hypogastric region angor, pendant pain, systemic pain, the pain over the hypochondriac region, waist abdomen cold type of pain.Through pharmacodynamic evaluation, it was demonstrated that the compound Chinese medicinal preparation prepared by said method has obvious anxiety, antidepressant, analgesic effect compared to blank group and matched group.And this Chinese medicine compound preparation method is simple, it is easy to large-scale production.
Description
Technical field
The invention belongs to the field of Chinese medicines, relate to a kind of Chinese patent medicine prescription treating menopathy.
Background technology
Menopathy (emmeniopathy) refer to the cycle with menstruation, menstrual period, through amount, through color, through the exception of matter, or with the moon
The disease that the symptom occurred through the cycle is characterized.Menopathy is the modal disease of gynecological, common are: after preceeded menorrhea, menstruation
Phase, irregular menstrual period, menorrhagia, hypomenorrhea, menostaxis, intermenstrual bleeding, metrorrhagia, dysmenorrhea, amenorrhea, the moon
Through the most all cards (dizziness during menstruation, diarrhea during menstruation, edema during menstruation, through sector-style rash, distending pain of the breast during menstruation, headache during menstruation, through row body
Bitterly, moody state during menstruation), menopausal syndrome, postmenopausal menstruation etc..
The traditional Chinese medical science is thought, the etiology and pathogenesis of menopathy is mainly damaged by excess of seven emotions or diseases caused by exogenous pathogenic factor six climate exopathogens, or congenital deficiency of kidney-QI, fecund exhaustion due to sexual indulgence,
Overstrain, makes internal organs impaired, kidney,liver,spleen malfunction, disorder of QI and blood, causes punching, wantonly two arteries and veins damages, sends out as menopathy.
The traditional Chinese medical science focuses on regulating menstruation to the treatment of menopathy.The method of traditional Chinese medical science regulating menstruation, should follow "Nei Jing" " cautiously grasping pathogenesis " and " careful Cha Yin
Sun place and adjust it, schedule to last with flat " objective.The concrete rule of regulating menstruation, have the kidney invigorating, hold up spleen, soothing the liver, QI and blood regulating different.
" go out all kidneys through water ", therefore the basis of regulating menstruation is at kidney.The kidney invigorating is to fill up essence and blood, and helps with supporing yang product, makes YIN and YANG in a relative equilibrium in kidney,
Essence and blood is the most prosperous, then menstruation self-regulated.Hold up spleen to be the source of benefiting vital QI and blood, based on spleen invigorating yang invigorating, make the strong fortune of temper, have under one's command and have the right,
Biochemical active, abundance of blood in CHONG meridian.Soothing the liver reaching irritability based on bar, it is intended that adjust the merit of its catharsis, make irritability to dredge, the sea of blood stores to overflow often to be had.
QI and blood regulating, at gas or at blood, disease is gas person, when to control gas, assistant is with nourshing blood and promoting blood circulation first should to differentiate disease；It is sick blood person,
Then to control blood, assistant is with QI invigorating circulation of qi promoting.Additionally, regulating menstruation is the most sick when distinguishing again, then gives birth to all patients through uncomfortable, adjust in the ban
Warp；Because his disease then causes through uncomfortable person, control him in the ban sick.
Summary of the invention
It is an object of the invention to, it is provided that one is treated women and produced because of menopathys such as dysmenorrhea, premenstrual syndrome, menopausal syndromes
The pain such as the headache of life, distending pain of the breast, pelvic pain, hypogastric region angor, pendant pain, systemic pain, the pain over the hypochondriac region, waist abdomen cold type of pain
The Chinese medicine compound of the mental symptoms such as shape and depressed emotion, fidgety irritability, insomnia and dreamful sleep.In order to realize above-mentioned task, the present invention takes
Following technical solution:
One, prescription
A kind of Chinese medicine compound treating menopathy, is made up of following crude drug: Radix Bupleuri 150～300g, Radix Angelicae Sinensis 150～300g, the Radix Paeoniae Alba
150～300g, Fructus Viticis 100～200g, Folium Ginkgo 100～200g, Cortex Magnoliae Officinalis 60～120g, Rhizoma Corydalis 60～120g, Radix Glycyrrhizae 60～
120g。
Optimum prescription is: Radix Bupleuri 225g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 225g, Fructus Viticis 150g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 90g, prolongs
Rhizoma Corydalis 90g, Radix Glycyrrhizae 90g.
Two, Fang Xie
Liver storing blood, the most main catharsis, have the meaning of " not cloudy and with positive ".Liver happiness bar reaches and dislikes depression, its passages through which vital energy circulates cloth side of body rib, follows
Few abdomen.Radix Bupleuri is pungent to be dissipated, main enter edema caused by disorder of QI, merit arrogates to oneself bar and reaches irritability and dredge pent-up, with for monarch drug.The pungent fragrance of Radix Angelicae Sinensis is opened, and walks and does not keeps,
Sweet Wen Zhirun, blood-supplementing blood-nourishing, call it " the gas medicine in blood "；The acid of Radix Paeoniae Alba property, yin fluid astringing, nourishing blood to suppress the hyperactive liver, to keep and do not walk, the two closes
With, pungent and only dissipate, acid and only receive, one opens a conjunction, and sound is suitable, helps Radix Bupleuri to solve the stasis of Liver Channel altogether, and line increment gas is lived
Effect of blood and relieving pain, is all ministerial drug.Fructus Viticis is pungent to be dissipated, bitter, be slightly cold, enter Liver Channel, have effect of wind-expelling pain-stopping, kind wind syndrome of head of dispelling；
Folium Ginkgo, astringes the lung, relievings asthma, promoting blood circulation and stopping pain；Cortex Magnoliae Officinalis is warm in nature, pungent, bitter, enters spleen, stomach, large intestine channel, dampness expectorant, and the therapeutic method to keep the adverse QI flowing downwards is removed
Full；Rhizoma Corydalis is warm in nature, pungent, bitter, returns liver, spleen channel, invigorate blood circulation, circulation of qi promoting, pain relieving, be all adjuvant drug.Radix Glycyrrhizae is not only for adjuvant drug but also for making
Medicine, coordinating the actions of various ingredients in a prescription, strengthening the spleen and in.
The combination of above-mentioned all herbal medicines makes each efficacy of drugs produce synergism, for the menopathy cause of disease and symptom, uses the traditional Chinese medical science to take orally
Method, dispersing the stagnated live-QI to relieve the stagnation of QI, promoting the circulation of QI to relieve pain.For treating women because of pain produced by dysmenorrhea, premenstrual syndrome, menopausal syndrome
Shape and mental symptom.
Three, preparation method
(1) Radix Bupleuri, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Fructus Viticis, Folium Ginkgo, Cortex Magnoliae Officinalis, Rhizoma Corydalis, Radix Glycyrrhizae eight taste medical material in prescription are cleaned, only
After system, Radix Bupleuri, Radix Angelicae Sinensis, Radix Glycyrrhizae mixing, the reflux, extract, that adds water 2 times, backflow merges 2 extracting solution in 2 hours every time, filters, filter
Liquid is concentrated into relative density and is 1.15-1.20(60 DEG C) clear paste, standby；
(2) the Radix Paeoniae Alba, Fructus Viticis, Folium Ginkgo, Cortex Magnoliae Officinalis, Rhizoma Corydalis mixing, add 65% alcohol reflux 2 times, and backflow 2 is little every time
Time merge 2 extracting solution, filter, filtrate recycling ethanol, be concentrated into relative density and be 1.15-1.20(60 DEG C) clear paste, standby；
Will (1) and (2) in clear paste merge, after adding appropriate amount of starch mixing, be dried, pelletize.
This composition of medicine can make tablet, capsule, pill or granule.
Detailed description of the invention
The present invention is described in further detail for the instantiation be given below in conjunction with inventor.
Embodiment 1
According to technical scheme, applicant makes capsule to after prescription medicinal material extract, specifically includes the following step:
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 225g, Fructus Viticis 150g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 90g.
Preparation method: (1) by clean to Radix Bupleuri in prescription, Radix Angelicae Sinensis, the Radix Paeoniae Alba, Fructus Viticis, Folium Ginkgo, Cortex Magnoliae Officinalis, Rhizoma Corydalis, Radix Glycyrrhizae eight taste medical material
After choosing, clean system, Radix Bupleuri, Radix Angelicae Sinensis, Radix Glycyrrhizae mixing, the reflux, extract, that adds water 2 times, backflow merges 2 extracting solution, filter for 2 hours every time
Crossing, filtrate is concentrated into relative density and is 1.15-1.20(60 DEG C) clear paste, standby；
(2) the Radix Paeoniae Alba, Fructus Viticis, Folium Ginkgo, Cortex Magnoliae Officinalis, Rhizoma Corydalis mixing, add 65% alcohol reflux 2 times, and backflow 2 is little every time
Time merge 2 extracting solution, filter, filtrate recycling ethanol, be concentrated into relative density and be 1.15-1.20(60 DEG C) clear paste, standby；
Will (1) and (2) in clear paste merge, after adding appropriate amount of starch mixing, be dried, pelletize, be dried；
(4) adding moderate lubrication agent after granulate, fill is in No. 0 capsule.
Usage: three times a day, each 4 are administered orally.
Embodiment 2
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 300g, Fructus Viticis 200g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 3
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 150g, Fructus Viticis 100g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 4
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 150g, Fructus Viticis 100g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 5
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 150g, Fructus Viticis 100g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 6
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 225g, Fructus Viticis 150g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 7
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 225g, Fructus Viticis 150g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 8
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 225g, Fructus Viticis 150g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 9
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 300g, Fructus Viticis 200g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 10
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 300g, Fructus Viticis 200g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 11
Prescription: Radix Bupleuri 150g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 300g, Fructus Viticis 200g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 12
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 225g, Fructus Viticis 200g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 13
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 225g, Fructus Viticis 200g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 14
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 225g, Fructus Viticis 200g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 15
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 300g, Fructus Viticis 100g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 16
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 300g, Fructus Viticis 100g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 17
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 300g, Fructus Viticis 100g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 18
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 150g, Fructus Viticis 150g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 19
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 150g, Fructus Viticis 150g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 20
Prescription: Radix Bupleuri 225g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 150g, Fructus Viticis 150g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 21
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 300g, Fructus Viticis 150g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 22
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 300g, Fructus Viticis 150g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 23
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 150g, Radix Paeoniae Alba 300g, Fructus Viticis 150g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 24
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 150g, Fructus Viticis 200g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
Embodiment 25
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 150g, Fructus Viticis 200g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 26
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 150g, Fructus Viticis 200g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 27
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 225g, Fructus Viticis 100g, Folium Ginkgo 100g, Cortex Magnoliae Officinalis 120g, Rhizoma Corydalis 90g,
Radix Glycyrrhizae 120g.
Preparation method and usage are with embodiment 1.
Embodiment 28
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 225g, Fructus Viticis 100g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 60g, Rhizoma Corydalis 120g,
Radix Glycyrrhizae 60g.
Preparation method and usage are with embodiment 1.
Embodiment 29
Prescription: Radix Bupleuri 300g, Radix Angelicae Sinensis 300g, Radix Paeoniae Alba 225g, Fructus Viticis 100g, Folium Ginkgo 200g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 60g,
Radix Glycyrrhizae 90g.
Preparation method and usage are with embodiment 1.
For proving the practicality of this patent further, drug nomenclature of the present invention is " through flat capsule ", and inventor passes through pharmacological evaluation pair
Compound preparation prepared by this technique carries out evaluating drug effect, as follows:
One, test material
1. test drug: through flat capsule, specification: 0.4g/ grain, every is equivalent to crude drug 1.245g, and lot number: 121101, by Yang Ling
The self-control of Dong Ke Mildison pharmaceutical Co. Ltd.
2. comparison medicine: fragrant Chinese herbaceous peony granule, specification: 4g/ bag, lot number: 12120301, sea, Yangzijiang Pharmaceutical Group Sichuan Rong's Pharmaceutical has
Limit company produces.
3. test equipment: Sartorius-BP210S electronic balance, Sartorius company of Germany；Sartorius-BL1500 electronic balance,
Sartorius company of Germany.PMT-100 Elevated plus-maze and video analytic system, Chengdu TME Technology Co., Ltd..
4. experimental animal: Sprague Dawley rat, SPF level, female, body weight 200～230g；ICR mice, SPF level, body
Weigh 18～22g；Thered is provided by Xi'an Jiaotong University Medical College's Experimental Animal Center, the animal certificate of competency (SYXK2007-001).
Animal sub-cage rearing, eats solid feed, and freely, feedstuff is provided drinking water by Xi'an Jiaotong University Medical College's Experimental Animal Center.Room temperature
18～25 DEG C, relative humidity 50%～70%.
Two, statistical disposition
Experimental data is with mean ± standard deviationRepresent.Use SPSS13.0 statistics software processes, the comparison of mean between group
Using t inspection, P ＜ represents that difference has statistical significance.
Three, test method and result
1. the impact (forced swimming method) on rat antidepressant effect
Taking healthy SD strain rats 50, female, body weight 200～230g, Stochastic Equilibrium is divided into 5 groups, often group 10: 1.
Blank group；2. through flat capsule small dose group (60mg/kg)；3. dosage group (120mg/kg) in flat capsule；4. through flat glue
Capsule heavy dose group (180mg/kg)；5. fragrant Chinese herbaceous peony granule group (100mg/kg).Packet gastric infusion, gastric infusion volume is 10ml/kg,
Once a day, continuous 10 days.It is administered the 9th day and rat is positioned in the water vat of depth of water 30cm, water temperature 25 DEG C, test 15min
After in 30 DEG C of room temperatures natural drying.Test in rat is positioned over after within 10th day, being administered 30 minutes above-mentioned water vat.Measure
Each group rat keeps the floating motionless time in 5min.Each administration group compares with blank group, the results are shown in Table 1.
The table 1 impact on rat forced swimming test
Note: each administration group compares with blank group: * P < 0.05, * * P < 0.01
Table 1 result shows, in rat forced swimming test, each administration group compares with blank group, through flat capsule (big or middle,
Low dose of) rat keeps the floating motionless time to shorten more apparent (* * P < 0.01) in 5min, and point out and make through flat Capsules On Anti depression
With being better than fragrant Chinese herbaceous peony granule.
2. the impact (outstanding tail method) on mice antidepressant effect
By ICR Strains of Mouse 50, female, body weight 18-22g, Stochastic Equilibrium is divided into 8 groups, often group 10: the most blank right
According to group；2. through flat capsule small dose group (60mg/kg)；3. dosage group (120mg/kg) in flat capsule；4. through big dose of flat capsule
Amount group (180mg/kg)；5. fragrant Chinese herbaceous peony granule group (100mg/kg).Packet gastric infusion, gastric infusion volume is 10ml/kg, often
It once, continuous 10 days.After last is administered 30 minutes, mice, is made in stand edge in immobilization with adhesive tape at tail point 2cm
Mice head hangs downwards.Not record during mice activity, starts stopwatch when occurring motionless, the time that in accumulative 6min, mice is motionless.
Each administration group compares with blank group, the results are shown in Table 2.
The table 2 impact on Tail suspension test
Note: each administration group compares with blank group: * P < 0.05, * * P < 0.01
Table 2 result shows, in Tail suspension test, each administration group compares with blank group, mice in flat Capsules group 6min
The motionless time shortens more apparent (* * P < 0.01, * P < 0.05), and prompting has antidepressant effect through flat capsule.
3. the impact (Elevated plus-maze method) on rat angst resistance effect
Taking healthy SD strain rats 80, female, body weight 200-230g, Stochastic Equilibrium is divided into 8 groups, often group 10: 1. empty
White matched group；2. through flat capsule small dose group (60mg/kg)；3. dosage group (120mg/kg) in flat capsule；4. through flat capsule
Heavy dose of group (180mg/kg)；5. fragrant Chinese herbaceous peony granule group (100mg/kg).Packet gastric infusion, gastric infusion volume is 10ml/kg,
Once a day, continuous 10 days.Last be administered after 30 minutes carry out elevated plus-maze test (PMT-100 Elevated plus-maze,
Chengdu TME Technology Co., Ltd.).Before elevated plus-maze model (EPM) experiment starts, first by every rat individually
Put in a 60cm × 60cm × 35cm plastic casing, be immediately placed in the central platform of EPM after being allowed to freely probe into 5min,
Make its head just to an open arms, after release, i.e. start to record the following active situation of rat in 5min: enter open arms time
Number (OE)；Enter the number of times (CE) of closure arm；Enter the time (OT) of open arms, unit: s；Enter the time (CT) of closure arm,
Unit: s.Calculate respectively according to data above: enter open arms and the total degree (OE+CE) of closure arm, represent rat with this
Mobility；The number of times entering open arms accounts for the ratio always entering arm number of times: OE%=OE/ (OE+CE) × 100%；Enter open arms
Time account for the ratio always entering the arm time: OT%=OT/ (OT+CT) × 100%, using OE% and OT% as the treatment of angst resistance effect
Effect deliberated index, each administration group compares with blank group, the results are shown in Table 3.
The table 3 impact on rat elevated plus-maze test
Note: each administration group compares with blank group: * P < 0.05, * * P < 0.01
Table 3 result shows, in rat elevated plus-maze test, each administration group compares with blank group, in flat capsule dosage,
Heavy dose of group, fragrant Chinese herbaceous peony granule group open arms/at arm holdup time percentage ratio (OT%) significantly raised (* P < 0.05, * * P < 0.01),
Dosage, heavy dose of group open arms/enter percentage of time (OE%) the most significantly raised (* P < 0.05), prompting at arm in flat capsule
In flat capsule, dosage, heavy dose have certain angst resistance effect.
4. mice thermostimulation is caused the impact (hot plate method) of pain reaction
By ICR Strains of Mouse, female, body weight 18～22g, after adapting to laboratory environment, use thermostatic water-circulator bath metal buckets,
Temperature temperature control, at 55 DEG C, first measures the threshold of pain (i.e. latency of pain response refers to that mice contact hot plate is to the time licking metapedes).Pain is anti-
Should be qualified Mus in 5～30S incubation period, select qualified mice 80 (failure need not), numbering of weighing, Stochastic Equilibrium divides
It is 5 groups, often group 10.1. blank group；2. through flat capsule small dose group (60mg/kg)；3. dosage group in flat capsule
(120mg/kg)；4. through flat capsule heavy dose group (180mg/kg)；5. fragrant Chinese herbaceous peony granule group (100mg/kg).Gastric infusion every day
Once, continuous 10 days, mice was placed on the hot plate of 55 DEG C the mensuration mice threshold of pain by last respectively after being administered 30 minutes and 60 minutes,
It was limited with 60 seconds, obtains the threshold of pain of each treated animal different time, compare before being administered, the results are shown in Table 4.
Table 4 causes the impact of pain reaction (hot plate method) to mice thermostimulation
Note: compare before being administered: * P ＜ 0.05, * * P ＜ 0.01
Table 4 result shows: within 30 minutes, 60 minutes after flat Capsules group is administered, have significant difference with comparing before administration
(* * P < 0.01).Fragrant Chinese herbaceous peony granule group is administered latter 60 minutes has significant difference (* P < 0.05, * * P < 0.01) with comparing before administration.
Result is pointed out: the above-mentioned mice pain caused thermostimulation through flat capsule has significant analgesic activity, and fragrant Chinese herbaceous peony particle effect is taken second place.
5. mice chemical stimulation is caused the impact (writhing method) of pain reaction
By ICR Strains of Mouse 80, female, body weight 18-22g, numbering of weighing after adapting to laboratory environment, Stochastic Equilibrium is divided into
5 groups, often group 10,1. blank group；2. through flat capsule small dose group (60mg/kg)；3. dosage group in flat capsule
(120mg/kg)；4. through flat capsule heavy dose group (180mg/kg)；5. fragrant Chinese herbaceous peony granule group (100mg/kg).Packet gastric infusion,
Gastric infusion volume is 10ml/kg, once a day, and continuous 10 days.After last is administered 30 minutes, mouse peritoneal injects 0.6% acetic acid
(0.2ml/ only), the writhing number of times of every mice and the mice number of elements of writhing response occurs in observing 15 minutes, each administration group and sky
White matched group compares, and the results are shown in Table 5.
Table 5 causes the impact of pain reaction (writhing method) to mice chemical stimulation
Note: each group compares with blank group: * * P ＜ 0.01
Table 5 result shows, the acetic acid 0.2ml/ of mouse peritoneal injection 0.6% only, can cause mouse peritoneal pain, occur that writhing is anti-
Should.After administration, test 1 group, test 2 groups of pain reaction that can substantially suppress mice to occur (* * P < 0.01).Result shows:
The mice pain that chemical stimulation is caused by above-mentioned prescription has significant analgesic activity.In flat capsule, dosage, heavy dose of group effect are excellent
In fragrant Chinese herbaceous peony granule group and through flat capsule small dose group.
Claims (3)
1. the Chinese medicine composition treating menopathy, it is characterised in that: its active component be by with
Lower weight crude drug forms, Radix Bupleuri 150～300g, Radix Angelicae Sinensis 150～300g, and the Radix Paeoniae Alba 150～300g is climing
Fructus Vitics Simplicifoliae 100～200g, Folium Ginkgo 100～200g, Cortex Magnoliae Officinalis 60～120g, Rhizoma Corydalis 60～120g, sweet
Grass 60～120g.
2. the Chinese medicine composition treating menopathy as claimed in claim 1, it is characterised in that it is lived
Property composition is made up of following preferred weight crude drug: Radix Bupleuri 225g, Radix Angelicae Sinensis 225g, Radix Paeoniae Alba 225g,
Fructus Viticis 150g, Folium Ginkgo 150g, Cortex Magnoliae Officinalis 90g, Rhizoma Corydalis 90g, Radix Glycyrrhizae 90g.
3. the use in the medicine of preparation treatment menopathy of the Chinese medicine composition described in claim 1 or 2
On the way.
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