CN103655661A - Method for promoting extraction of active components in folium artemisiae argyi - Google Patents
Method for promoting extraction of active components in folium artemisiae argyi Download PDFInfo
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- CN103655661A CN103655661A CN201210349439.6A CN201210349439A CN103655661A CN 103655661 A CN103655661 A CN 103655661A CN 201210349439 A CN201210349439 A CN 201210349439A CN 103655661 A CN103655661 A CN 103655661A
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- folium artemisiae
- artemisiae argyi
- extraction
- argyi
- active components
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Abstract
The invention relates to a method for promoting extraction of active components in folium artemisiae argyi. The active components in the folium artemisiae argyi can be extracted with a solvent and subjected to further processing. The extraction of the folium artemisiae argyi generally uses water or ethanol as the solvent, and an ultrasonic action is further used, thereby enhancing the yields of the main components.
Description
Technical field
After active component useable solvents in Folium Artemisiae Argyi is extracted, more further process.The extraction of Folium Artemisiae Argyi generally adopts water or ethanol as solvent, and the present invention further uses ul-trasonic irradiation, has improved the yield of each main component.
Background technology
Folium Artemisiae Argyi is the dried leaves of feverfew Chinese mugwort, is herbaceos perennial, is a kind of conventional Chinese herbal medicine, has the effects such as dispersing cold for relieving pain, warming the meridian for stopping bleeding, anti-bacteria and anti-virus.Be distributed in the ground such as Heilungkiang, Jilin, Liaoning, Hebei, Shandong, Anhui, Jiangsu, Zhejiang, Guangdong, Guangxi, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Yunnan, Shaanxi, Gansu.Although Folium Artemisiae Argyi is widely distributed throughout the country, wild resource is compared with horn of plenty, and its quality is affected by often in the place of production.In the Song dynasty, the Hai Ai that north that Folium Artemisiae Argyi is produced with volt road, Tangyin, Henan Chinese mugwort and Ningbo of Zhejiang are produced is for well, and Song is with so far, and people generally believe that Folium Artemisiae Argyi take Qichun, Hubei product person as good.
Folium Artemisiae Argyi is warm in nature, and acrid in the mouth, hardship are slightly poisonous.The effect with dispersing cold for relieving pain, warming the meridian for stopping bleeding.The chemical composition of Folium Artemisiae Argyi mainly contains volatile oil, flavonoid, Folium eucalypti globueli (Eucalyptus globulus Labill.) alkanes, triterpenes etc.Modern pharmacological research shows that Folium Artemisiae Argyi has anti-bacteria and anti-virus, the relieving cough and expelling phlegm of relievining asthma, hemostasis and the effect such as anticoagulation, antiallergic, calmness, immunity, hepatoprotective function of gallbladder promoting and complement activation.
After active component useable solvents in Folium Artemisiae Argyi is extracted, more further process.
Summary of the invention
The extraction of Folium Artemisiae Argyi generally adopts water or ethanol as solvent, and the present invention further uses ul-trasonic irradiation, has improved the yield of each main component.
The specific embodiment
Embodiment mono-
Matched group: accurately take the dry Folium Artemisiae Argyi of 100g and pack in volatile oil extractor, carry out vapor distillation, filtrate is filtered, then in standardize solution to 1000 bottle, shake up, polysaccharide solution obtained.Use petroleum ether extraction volatile oil simultaneously, after distilling under reduced pressure, with anhydrous sodium sulfate drying, obtain volatile oil.Measure argyi leaf polysaccharide extraction rate and volatile oil extraction rate.
The assay method of argyi leaf polysaccharide extraction rate and volatile oil extraction rate: adopt sulphuric acid one anthrone method to measure polyoses content.
Quality (the g)/Folium Artemisiae Argyi quality (g) * 100% of volatile oil extraction rate (%)=volatile oil.
Ultrasound wave group: accurately take the dry Folium Artemisiae Argyi of 100g and pack in volatile oil extractor, carry out vapor distillation, apply 20kHz ultrasound wave simultaneously with supersonic generator, filtrate is filtered, then in standardize solution to 1000 bottle, shake up, polysaccharide solution obtained.Use petroleum ether extraction volatile oil simultaneously, after distilling under reduced pressure, with anhydrous sodium sulfate drying, obtain volatile oil.
With said method, measure argyi leaf polysaccharide extraction rate and volatile oil extraction rate.
Result:
| Group | Argyi leaf polysaccharide (%) | Volatile oil (%) |
| Matched group | 0.428 | 2.56 |
| Ultrasound wave group | 0.587 | 3.03 |
Ultrasound wave group has obtained better extraction effect.
Embodiment bis-
Matched group: test sample extractum is pulverized the dry Folium Artemisiae Argyi of 500g, with certain density ethanol (65%, 75%, 95%) dipping, each 4L, 24h, totally 2 times.Merging filtrate, sucking filtration.Medicinal residues are discarded, and gained filtrate, through rotary evaporation distilling under reduced pressure, is reclaimed ethanol, makes extractum.Precision takes pretreated Folium Artemisiae Argyi extractum, and dry extract 100mg is placed in 100ml volumetric flask, with ethanol dilution, to scale, shakes up, and obtains.Measure general flavone content.
Ultrasound wave group: test sample extractum is pulverized the dry Folium Artemisiae Argyi of 500g, with certain density ethanol (65%, 75%, 95%) dipping, each 4L, 24h applies 20kHz ultrasound wave with supersonic generator simultaneously, totally 2 times.Merging filtrate, sucking filtration.Medicinal residues are discarded, and gained filtrate, through rotary evaporation distilling under reduced pressure, with receiving ethanol, is made extractum.Precision takes pretreated Folium Artemisiae Argyi extractum, and dry extract 100mg is placed in 100ml volumetric flask, with ethanol dilution, to scale, shakes up, and obtains.Measure general flavone content.
Result: matched group total flavones is 4.57%, ultrasound wave group is 5.38%, ultrasound wave group has obtained better extraction effect.
Embodiment tri-
Matched group: the extraction of argyi leaf polysaccharide, get Folium Artemisiae Argyi 50g and add dehydrated alcohol submergence, in 85 ℃ of water bath with thermostatic control reflux extraction 80min, to filter, Folium Artemisiae Argyi is dry under natural conditions, add purified water 400ml (solid-liquid ratio is 1: 8), 96 ℃ of water bath with thermostatic control cocurrent flow extraction 2h, sucking filtration obtains filtrate, and medicinal residues add 400ml distilled water extraction 2h again, merge secondary filtrate, in Rotary Evaporators, 50-60 ℃ concentrates to obtain Folium Artemisiae Argyi water extract.Folium Artemisiae Argyi water extract and dehydrated alcohol are mixed for by volume 1: 3, and constantly stir, in centrifuge with the centrifugal 3min of 4500r/min, precipitation is washed 3 times with dehydrated alcohol, centrifugal, obtain precipitation, precipitation is put in vacuum drying oven, 60 ℃ of dry 90min, obtain argyi leaf polysaccharide, it is 10mg/ml that argyi leaf polysaccharide powder is made into concentration with distilled water, filtration sterilization, sealing, standby in room temperature preservation.Measure the content of argyi leaf polysaccharide.
Ultrasound wave group: the extraction of argyi leaf polysaccharide, get Folium Artemisiae Argyi 50g and add dehydrated alcohol submergence, in 85 ℃ of water bath with thermostatic control reflux extraction 80min, with supersonic generator, apply 20kHz ultrasound wave simultaneously, filter, Folium Artemisiae Argyi is dry under natural conditions, adds purified water 400ml (solid-liquid ratio is 1: 8), 96 ℃ of water bath with thermostatic control reflux extraction 2h, sucking filtration obtains filtrate, medicinal residues add 400ml distilled water extraction 2h again, merge secondary filtrate, and in Rotary Evaporators, 50-60 ℃ concentrates to obtain Folium Artemisiae Argyi water extract.By Folium Artemisiae Argyi water extract and dehydrated alcohol by volume 1:3 mix, and constantly stir, in centrifuge with the centrifugal 3min of 4500r/min, precipitation is washed 3 times with dehydrated alcohol, centrifugal, obtain precipitation, precipitation is put in vacuum drying oven, 60 ℃ of dry 90min, obtain argyi leaf polysaccharide, it is 10mg/ml that argyi leaf polysaccharide powder is made into concentration with distilled water, filtration sterilization, sealing, standby in room temperature preservation.Measure the content of argyi leaf polysaccharide.
Result: matched group argyi leaf polysaccharide is 2.87%, ultrasound wave group is 3.37%, ultrasound wave group has obtained better extraction effect.
Claims (2)
1. select the active component in suitable solvent extraction Folium Artemisiae Argyi.
2. with supersonic generator, apply 20kHz ultrasound wave to improve the yield of the active component in Folium Artemisiae Argyi simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210349439.6A CN103655661A (en) | 2012-09-20 | 2012-09-20 | Method for promoting extraction of active components in folium artemisiae argyi |
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| CN201210349439.6A CN103655661A (en) | 2012-09-20 | 2012-09-20 | Method for promoting extraction of active components in folium artemisiae argyi |
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| CN103655661A true CN103655661A (en) | 2014-03-26 |
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| CN201210349439.6A Pending CN103655661A (en) | 2012-09-20 | 2012-09-20 | Method for promoting extraction of active components in folium artemisiae argyi |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462690A (en) * | 2015-11-18 | 2016-04-06 | 广州暨南生物医药研究开发基地有限公司 | Argy wormwood leaf essence oil and preparing method thereof |
| CN111588741A (en) * | 2020-05-13 | 2020-08-28 | 扬州大学 | Preparation method and application of folium artemisiae argyi extract |
-
2012
- 2012-09-20 CN CN201210349439.6A patent/CN103655661A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105462690A (en) * | 2015-11-18 | 2016-04-06 | 广州暨南生物医药研究开发基地有限公司 | Argy wormwood leaf essence oil and preparing method thereof |
| CN105462690B (en) * | 2015-11-18 | 2019-02-15 | 广东暨晴生物医药科技有限公司 | A kind of P-Cymene and preparation method thereof |
| CN111588741A (en) * | 2020-05-13 | 2020-08-28 | 扬州大学 | Preparation method and application of folium artemisiae argyi extract |
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Application publication date: 20140326 |