CN103651132A - Rapid rooting medium for peanut tissue culture seedlings and culture method - Google Patents
Rapid rooting medium for peanut tissue culture seedlings and culture method Download PDFInfo
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Abstract
The invention relates to a rapid rooting medium for peanut tissue culture seedlings and a culture method. The medium is added the following components on the basis of a MS basic medium: indolebutyric acid, naphthylacetic acid, gibberellins, inositol and vitamin B1, wherein the pH of the medium is 5.8. The invention also relates to a culture method for rapidly rooting peanut tissue culture seedlings by utilizing the medium. By cultured by utilizing the medium provided by the invention, the peanut tissue culture seedlings have the rooting rate of 100% and the transplanting survival rate of 90% or more, and thus the problem that peanut tissue culture seedlings are difficult to root is solved, and the medium and the culture method establish a base for peanut tissue culture and genetic transformation.
Description
Technical field
The present invention relates to plant Rapid Rooting medium and cultural method, particularly peanut tissue culture seedlings Rapid Rooting medium and cultural method, belong to crop seedling technical field.
Background technology
Peanut is important economic crops, and the status in Chinese national economy and agricultural production is day by day remarkable.But peanut organize cultivation and paddy rice, corn, rape, etc. crop and horticultural crop compare, there is very large gap, main cause be because peanut tissue culture seedling rooting difficult, take root slowly, and the root bearing mostly is lopsided root, thereby causes group training transplantation of seedlings survival rate very low.
In the last few years, the tissue-culturing rapid propagation of peanut had been obtained greater advance, had substantially broken through the bottleneck of peanut inducing clumping bud, produced the method for Multiple Buds by induction peanut hypocotyl, and many peanut varieties have all obtained a large amount of Multiple Buds.Ensuing bottleneck problem is exactly peanut tissue culture seedling rooting.Because peanut tissue culture seedling rooting is difficult, people have found out various ways and have solved this problem, wherein more efficiently a kind of method is exactly grafting, on stem using healthy and strong group training seedling as scion grafting to wild-type peanut, by the cultivation of a period of time, can access the grafting of some, respite the problem of peanut tissue culture seedling rooting difficulty.But grafting is different from seedling after all; the budlet that often has wild-type peanut oneself at the base portion as the wild-type peanut seedling of stock is longer, and growing way is far longer than grafting, fights for nutrition with grafting; so will cut off at any time these budlets, thereby increase researcher's workload.Moreover grafting survival rate is not very high, only has 60% left and right.
At present existing part Study personnel have made and have been conducive to the medium that peanut is taken root, as Chinese patent literature CN102577981A(application number 201210079420.4) method that discloses a kind of strong sprout is effective, rooting rate is high, quality of rooting is good transgenic peanuts group training seedling strong sprout, taken root, comprise the steps: that (1) will induce the peanut tissue culture seedlings that differentiates Multiple Buds to transfer to upper 20 day of strong seedling culture base A, within 10 days, change a subculture, the component of described strong seedling culture base A is MS medium, and hormone used is basic element of cell division 6-BA; (2) the group training seedling growing to more than 1.5cm is divided into individual plant, move to strong seedling culture base B upper 20～25 days, within 10 days, change a subculture, the composition of described strong seedling culture base B is MS medium, the basic element of cell division 6-BA that hormone used is 0.4mg/L and the growth hormone NAA of 0.1mg/L; (3) treat that seedling grows to 2.5～4cm, move to root media and take root, the composition of described root media is 1/2MS medium, and hormone used is growth hormone IBA and NAA, and concentration is respectively 3mg/L and 0.3mg/L.
Chinese patent literature CN101411305A(application number 200810219406.3) method of cultivating peanut Vitro Quick Reproduction is disclosed, the method is to cultivate on the basis of included following four steps (1) the explant surface sterilization of peanut in-vitro propagate, the generation of (2) evoking adventive bud, the cultivation of (3) Elongation of adventitious bud and (4) culture of rootage at existing tissue, and bud elongation medium and condition of culture in the indefinite bud generation medium in step (2) and evoking adventive bud condition of culture and step (3) are optimized to improvement.The present invention adopts 4-forchlorfenuron as main component, promotes plant tissue generation indefinite bud, compares with the existing material that promotes indefinite bud to occur, activity is low, effect is showing, and in indefinite bud generating process, the approaching tissue of cultivating basal plane of explant there will not be black, promotes that blastogenesis is long.The inventive method promotes the method for Elongation of adventitious bud to compare with existing, and without increasing special equipment, gibberellin and cytokinin working concentration are little, and cost is low, extends the short and effect of cultivation required time and is showing.
In technical scheme, the Multiple Buds of acquisition need to be cultivated a period of time in strong seedling culture base or bud elongation medium, just can be used for taking root, thereby be unfavorable for the fast seedling growing of peanut after seedling is grown up.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of peanut tissue culture seedlings Rapid Rooting medium and cultural method are provided.
Terminological interpretation
IBA: indolebutyric acid;
NAA: methyl α-naphthyl acetate;
GA
3: gibberellin GA
3.
Technical solution of the present invention is as follows:
A peanut tissue culture seedlings Rapid Rooting medium adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.6～0.8mg, methyl α-naphthyl acetate (NAA) 0.08～0.12mg, gibberellin GA
3(GA
3) 0.8～1.2mg, inositol 80～120mg, Cobastab
1(VB
1) 0.8～1.2mg, pH5.8.
Preferred according to the present invention, on the basis of every liter of MS minimal medium, add following component:
Indolebutyric acid (IBA) 0.65～0.75mg, methyl α-naphthyl acetate (NAA) 0.09～0.11mg, gibberellin GA
3(GA
3) 0.9～1.1mg, inositol 90～110mg, Cobastab
1(VB
1) 0.9～1.1mg, pH5.8.
MS minimal medium is the conventional medium in this area, and component is as follows:
NH
4nO
31.65g/L, KNO
31.9g/L, CaCl
22H
2o0.44g/L, MgSO
47H
2o0.37g/L, KH
2pO
40.17g/L, KI0.83mg/L, H
3bO
36.2mg/L, MnSO
44H
2o22.3mg/L, ZnSO
47H
2o8.6mg/L, Na
2moO
42H
2o0.25mg/L, CuSO
45H
2o0.025mg/L, CoCl
26H
2o0.025mg/L, FeSO
27H
2o27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30g/L, agar 7.2g/L.
A peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is cut to 1.0～2.0 centimetres, be placed in peanut tissue culture seedlings Rapid Rooting medium, at 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate 20～30 days, bear a large amount of root systems.
As indivedual groups of training seedlings can not bear root system, only 1.0-1.5 centimetre of its tip need be cut, again take root.To there is the plantlet of transplant of better root system, normal management.Not too flourishing plant many growth a period of times on root media of root system can be improved survival rate greatly.
Preferred according to the present invention, the incubation step of described peanut tissue culture seedlings is as follows:
Peanut seed is placed in to aseptic triangular flask, and first adding percent by volume is 75% alcohol immersion 1 minute, takes out, the mercuric chloride solution that immersion mass percent is 0.1%, sterilizing 20 minutes, takes out, then with aseptic water washing, remove residual mercuric chloride, remove kind of a skin, be placed in 1/2MS medium, cultivate 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducing culture and sprout, cultivate 20～30 days and get final product;
Further preferred according to the present invention, described bud inducing culture adds following component on the basis of MS minimal medium:
Methyl α-naphthyl acetate (NAA) 0.7mg/L, 6-benzyl aminoadenine (6-BA) 8.0mg/L, pH5.8.
1/2MS medium is the conventional medium in this area, and component is as follows: except agar, each component content is half of MS minimal medium.
Beneficial effect
1, peanut tissue culture seedlings Rapid Rooting medium of the present invention, can make the rooting rate of peanut tissue culture seedlings reach 100%, transplanting survival rate reaches more than 90%, has solved the problem of peanut tissue culture seedling rooting difficulty, for the development of the cultivation of peanut tissue and genetic transformation is laid a good foundation.
2, in peanut tissue culture seedlings Rapid Rooting medium of the present invention, additionally added VB
1and inositol, these two kinds of vitamins combine use, for Rapid Rooting and the plant strain growth of peanut Multiple Buds, there is larger facilitation.
3, the more existing peanut tissue culture method of peanut tissue culture seedlings Rapid Rooting cultural method of the present invention is compared, omitted that Multiple Buds extends or strong sprout this process, the Multiple Buds that is directly 1.0-2.0 centimetre by length is positioned on root media takes root, when taking root, be extending again can strong sprout, formation with healthy and strong root system whole plant, greatly shorten the regeneration period of peanut Multiple Buds, simplified operating procedure, saved the time, man power and material.
Accompanying drawing explanation
Fig. 1 is peanut tissue culture seedlings;
Fig. 2 is the peanut tissue culture seedlings photo of taking root;
Fig. 3 is the healthy and strong root system photo that in embodiment 1, peanut tissue culture seedlings bears;
Fig. 4 is the healthy and strong root system photo that in embodiment 2, peanut tissue culture seedlings bears;
Fig. 5 is the healthy and strong root system photo that in embodiment 3, peanut tissue culture seedlings bears;
Fig. 6 is the healthy and strong root system photo that in embodiment 4, peanut tissue culture seedlings bears;
Fig. 7 is the healthy and strong root system photo that in embodiment 5, peanut tissue culture seedlings bears;
Fig. 8 is the root system photo that in comparative example 1, peanut tissue culture seedlings bears;
Fig. 9 is the root system photo that in comparative example 2, peanut tissue culture seedlings bears;
Figure 10 is the root system photo that in comparative example 3, peanut tissue culture seedlings bears.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated, but institute of the present invention protection domain is not limited to this.
Experiment material
Embodiment 1～3 adopts peanut varieties for ' flower educates 22 ', and it is ' rich spending No. 1 ' that embodiment 4 adopts peanut varieties, and the peanut varieties that embodiment 5 adopts is for ' Shandong spends 14 ', and above-mentioned kind is common commercially available kind.
Medium:
MS minimal medium, component is as follows:
NH
4nO
31.65g/L, KNO
31.9g/L, CaCl
22H
2o0.44g/L, MgSO
47H
2o0.37g/L, KH
2pO
40.17g/L, KI0.83mg/L, H
3bO
36.2mg/L, MnSO
44H
2o22.3mg/L, ZnSO
47H
2o8.6mg/L, Na
2moO
42H
2o0.25mg/L, CuSO
45H
2o0.025mg/L, CoCl
26H
2o0.025mg/L, FeSO
27H
2o27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30g/L, agar 7.2g/L.
1/2MS medium, component is as follows: except agar, each component content is half of MS minimal medium.
Bud inducing culture adds following component on the basis of MS minimal medium:
Methyl α-naphthyl acetate (NAA) 0.7mg/L, 6-benzyl aminoadenine (6-BA) 8.0mg/L, pH5.8.
Embodiment 1
A peanut tissue culture seedlings Rapid Rooting medium adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.7mg/L, methyl α-naphthyl acetate (NAA) 0.1mg/L, gibberellin GA
3(GA
3) 1.0mg/L, inositol 100mg/L, Cobastab
1(VB
1) 1.0mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings:
Select big or small uniformity ' flower is educated 22 ' seed and is placed in aseptic triangular flask, first adding percent by volume is 75% alcohol immersion 1 minute, take out, immersing mass percent is 0.1% mercuric chloride solution, rock continuously, sterilizing 20 minutes, take out, use aseptic water washing 4 times, wash away residual mercuric chloride, then the peanut seed after sterilizing is removed to kind of a skin, be placed in 1/2MS medium and cultivate after 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducing culture and sprout, after 20～30 days, obtain healthy and strong group training seedling (as shown in Figure 1);
A peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut to 1.0 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting medium, in 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate and within 7 days, can see that cutting part starts to expand and has the tip of a root to form, cultivate and within 25 days, have a large amount of normal roots to generate, and on root, also can constantly have lateral root to generate (as shown in Figure 2 and Figure 3).Be transplanted in flowerpot, normal management.
After testing, survival rate reaches 97%.
Embodiment 2
A peanut tissue culture seedlings Rapid Rooting medium adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.6mg/L, methyl α-naphthyl acetate (NAA) 0.08mg/L, gibberellin GA
3(GA
3) 0.8mg/L, inositol 80mg/L, Cobastab
1(VB
1) 0.8mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings as described in Example 1.
A peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut to 2.0 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting medium, in 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate and within 10 days, can see that cutting part starts to expand and has the tip of a root to form, cultivate and within 30 days, have a large amount of normal roots to generate, and on root, also can constantly have lateral root to generate (as shown in Figure 4).Be transplanted in flowerpot, normal management.
After testing, survival rate reaches 94%.
Embodiment 3
A peanut tissue culture seedlings Rapid Rooting medium adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.75mg/L, methyl α-naphthyl acetate (NAA) 0.11mg/L, gibberellin GA
3(GA
3) 1.1mg/L, inositol 110mg/L, Cobastab
1(VB
1) 1.1mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings as described in Example 1.
A peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut to 1.5 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting medium, in 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate and within 8 days, can see that cutting part starts to expand and has the tip of a root to form, cultivate and within 20 days, have a large amount of normal roots to generate, and on root, also can constantly have lateral root to generate (as shown in Figure 5).Be transplanted in flowerpot, normal management.
After testing, survival rate reaches 95%.
Embodiment 4
A peanut tissue culture seedlings Rapid Rooting medium adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.7mg/L, methyl α-naphthyl acetate (NAA) 0.1mg/L, gibberellin GA
3(GA
3) 1.0mg/L, inositol 100mg/L, Cobastab
1(VB
1) 1.0mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings:
The seed of selecting ' rich the spending No. 1 ' of big or small uniformity is placed in aseptic triangular flask, first adding percent by volume is 75% alcohol immersion 1 minute, take out, adding mass percent is 0.1% mercuric chloride solution, rock continuously, sterilizing 20 minutes, take out, use aseptic water washing 4 times, wash away residual mercuric chloride, then the peanut seed after sterilizing is removed to kind of a skin, be placed in 1/2MS medium and cultivate after 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducing culture and sprout, after 25 days, obtain healthy and strong group training seedling.
A peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut to 1.5 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting medium, in 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate and within 9 days, can see that cutting part starts to expand and has the tip of a root to form, cultivate and within 25 days, have a large amount of normal roots to generate, and on root, also can constantly have lateral root to generate (as shown in Figure 6).Be transplanted in flowerpot, normal management.
After testing, survival rate reaches 96%.
Embodiment 5
A peanut tissue culture seedlings Rapid Rooting medium adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.6mg/L, methyl α-naphthyl acetate (NAA) 0.12mg/L, gibberellin GA
3(GA
3) 1.2mg/L, inositol 80mg/L, Cobastab
1(VB
1) 0.8mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings:
Select big or small uniformity ' Shandong spends 14 ' seed to be placed in aseptic triangular flask, and first adding percent by volume is 75% alcohol immersion 1 minute, takes out, adding mass percent is 0.1% mercuric chloride solution, rocks continuously sterilizing 20 minutes, take out, use aseptic water washing 4 times, wash away residual mercuric chloride, then the peanut seed after sterilizing is removed to kind of a skin, be placed in 1/2MS medium and cultivate after 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducing culture and sprout, after 30 days, obtain healthy and strong group training seedling.
A peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut to 1.5 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting medium, in 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate and within 7 days, can see that cutting part starts to expand and has the tip of a root to form, cultivate and within 25 days, have a large amount of normal roots to generate, and on root, also can constantly have lateral root to generate (as shown in Figure 7).Be transplanted in flowerpot, normal management.
After testing, survival rate reaches 95%.
Comparative example 1
Root media adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.7mg/L, methyl α-naphthyl acetate (NAA) 0.1mg/L, gibberellin GA3(GA3) 1.0mg/L, vitamin B1 (VB1) 1.0mg/L, pH5.8.
Other experimental techniques are with embodiment 1.
Cultivate after 15 days, most of Multiple Buds can bear root system, and on average each bastem portion incision approximately generates 5～6 main roots.While continuing to be cultured to 25 days, main root approximately grows to 4～5 centimetres, only bears 4～5 little lateral roots above every main root, be transplanted in flowerpot, and normal management.
After testing, survival rate is 85%.
Comparative example 2
Root media adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.7mg/L, methyl α-naphthyl acetate (NAA) 0.1mg/L, gibberellin GA3(GA3) 1.0mg/L, inositol 100mg/L, sucrose 20g/L, pH5.8.
Other experimental techniques are with embodiment 1.
Cultivate after 15 days, most of Multiple Buds can bear root system, and on average each bastem portion incision approximately generates 5～6 main roots.While continuing to be cultured to 25 days, main root approximately grows to 4～5 centimetres, only bears 5～6 little lateral roots above every main root, plant in flowerpot, and normal management.
After testing, survival rate is 81%.
Comparative example 3
Root media adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.7mg/L, methyl α-naphthyl acetate (NAA) 0.1mg/L, gibberellin GA3(GA3) 1.0mg/L, sucrose 20g/L, pH5.8.
Other experimental techniques are with embodiment 1.
Cultivate after 15 days, most of Multiple Buds can bear root system, and on average each bastem portion incision approximately generates 3～4 main roots.While continuing to be cultured to 25 days, main root approximately grows to 3～4 centimetres, only bears 3～4 little lateral roots above every main root, be transplanted in flowerpot, and normal management.
After testing, survival rate is 73%.
Interpretation of result:
Through embodiment, interpretation of result shows, the rooting efficiency of this prescription of rooting medium is not subject to the impact of peanut genotype, and the group training seedling of whichever peanut varieties can comparatively fast grow healthy and strong root system on this root media.
Through comparative example, interpretation of result shows, add the ratio of taking root that vitamin B1 and inositol can obviously improve peanut tissue culture seedlings, and the lateral root bearing on main root is more simultaneously.
Claims (6)
1. a peanut tissue culture seedlings Rapid Rooting medium, is characterized in that, adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.6～0.8mg, methyl α-naphthyl acetate (NAA) 0.08～0.12mg, gibberellin GA
3(GA
3) 0.8～1.2mg, inositol 80～120mg, Cobastab
1(VB
1) 0.8～1.2mg, pH5.8.
2. peanut tissue culture seedlings Rapid Rooting medium as claimed in claim 1, is characterized in that, adds following component on the basis of every liter of MS minimal medium:
Indolebutyric acid (IBA) 0.65～0.75mg, methyl α-naphthyl acetate (NAA) 0.09～0.11mg, gibberellin GA
3(GA
3) 0.9～1.1mg, inositol 90～110mg, Cobastab
1(VB
1) 0.9～1.1mg, pH5.8.
3. peanut tissue culture seedlings Rapid Rooting medium as claimed in claim 1, is characterized in that, MS minimal medium component is as follows:
NH
4nO
31.65g/L, KNO
31.9g/L, CaCl
22H
2o0.44g/L, MgSO
47H
2o0.37g/L, KH
2pO
40.17g/L, KI0.83mg/L, H
3bO
36.2mg/L, MnSO
44H
2o22.3mg/L, ZnSO
47H
2o8.6mg/L, Na
2moO
42H
2o0.25mg/L, CuSO
45H
2o0.025mg/L, CoCl
26H
2o0.025mg/L, FeSO
27H
2o27.8mg/L, inositol 100mg/L, glycine 2mg/L, thiamine hydrochloride 0.1mg/L, puridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 20g/L, agar 7.2g/L.
4. a peanut tissue culture seedlings Rapid Rooting cultural method, is characterized in that, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is cut to 1.0～2.0 centimetres, be placed in peanut tissue culture seedlings Rapid Rooting medium claimed in claim 1, at 25 ℃, under 16 hours illumination/8 hour dark conditions, cultivate 20～30 days, transplant, normal management.
5. method as claimed in claim 4, is characterized in that, the incubation step of described peanut tissue culture seedlings is as follows:
Peanut seed is placed in to aseptic triangular flask, and first adding percent by volume is 75% alcohol immersion 1 minute, takes out, the mercuric chloride solution that immersion mass percent is 0.1%, sterilizing 20 minutes, takes out, then with aseptic water washing, remove residual mercuric chloride, remove kind of a skin, be placed in 1/2MS medium, cultivate 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducing culture and sprout, cultivate 20～30 days and get final product.
6. method as claimed in claim 5, is characterized in that, described bud inducing culture adds following component on the basis of MS minimal medium:
Methyl α-naphthyl acetate 0.7mg/L, 6-benzyl aminoadenine 8.0mg/L, pH5.8.
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| CN105494415A (en) * | 2016-01-19 | 2016-04-20 | 耿云强 | Rooting agent suitable for various plants and preparation method thereof |
| CN109809896A (en) * | 2017-11-22 | 2019-05-28 | 丹阳市陵口镇城墅蔬菜专业合作社 | Compost is used in a kind of plantation of crowndaisy chrysanthemum |
| CN110367124A (en) * | 2019-08-29 | 2019-10-25 | 淮北师范大学 | A method of building peanut cotylcdon regenerating system |
| CN110786245A (en) * | 2019-12-16 | 2020-02-14 | 山东省花生研究所 | Peanut tissue culture seedling root induction culture medium and preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105494415A (en) * | 2016-01-19 | 2016-04-20 | 耿云强 | Rooting agent suitable for various plants and preparation method thereof |
| CN109809896A (en) * | 2017-11-22 | 2019-05-28 | 丹阳市陵口镇城墅蔬菜专业合作社 | Compost is used in a kind of plantation of crowndaisy chrysanthemum |
| CN110367124A (en) * | 2019-08-29 | 2019-10-25 | 淮北师范大学 | A method of building peanut cotylcdon regenerating system |
| CN110367124B (en) * | 2019-08-29 | 2021-04-09 | 淮北师范大学 | Method for constructing peanut cotyledon regeneration system |
| CN110786245A (en) * | 2019-12-16 | 2020-02-14 | 山东省花生研究所 | Peanut tissue culture seedling root induction culture medium and preparation method and application thereof |
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