CN103651132B - Rapid rooting medium for peanut tissue culture seedlings and culture method - Google Patents
Rapid rooting medium for peanut tissue culture seedlings and culture method Download PDFInfo
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- CN103651132B CN103651132B CN201310654356.2A CN201310654356A CN103651132B CN 103651132 B CN103651132 B CN 103651132B CN 201310654356 A CN201310654356 A CN 201310654356A CN 103651132 B CN103651132 B CN 103651132B
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Abstract
The invention relates to a rapid rooting medium for peanut tissue culture seedlings and a culture method. The medium is added the following components on the basis of a MS basic medium: indolebutyric acid, naphthylacetic acid, gibberellins, inositol and vitamin B1, wherein the pH of the medium is 5.8. The invention also relates to a culture method for rapidly rooting peanut tissue culture seedlings by utilizing the medium. By cultured by utilizing the medium provided by the invention, the peanut tissue culture seedlings have the rooting rate of 100% and the transplanting survival rate of 90% or more, and thus the problem that peanut tissue culture seedlings are difficult to root is solved, and the medium and the culture method establish a base for peanut tissue culture and genetic transformation.
Description
Technical field
The present invention relates to plant Rapid Rooting substratum and cultural method, particularly peanut tissue culture seedlings Rapid Rooting substratum and cultural method, belong to crop seedling technical field.
Background technology
Peanut is important cash crop, and the status in Chinese national economy and agriculture production is day by day remarkable.But peanut tissue culture and paddy rice, corn, rape, etc. compared with crop and garden crop, there is very large gap, major cause is because of peanut tissue culture seedling rooting difficulty, takes root slowly, and the root born mostly is lopsided root, thus causes tissue cultured seedling transplanting survival rate very low.
In the last few years, the tissue culture fast-propagation of peanut achieved greater advance, substantially breached the bottleneck of peanut inducing clumping bud, and produced the method for Multiple Buds by induction peanut hypocotyl, many peanut varieties are obtained for a large amount of Multiple Buds.Ensuing bottleneck problem is exactly peanut tissue culture seedling rooting.Because peanut tissue culture seedling rooting is difficult, people have found out various way and have solved this problem, wherein more efficiently a kind of method is exactly grafting, using the tissue cultured seedling of stalwartness as scion grafting on the stem of wild-type peanut, the Graft of some amount can be obtained, the respite problem of peanut tissue culture seedling rooting difficulty by the cultivation of for some time.But Graft is different from seedling after all; the budlet often having wild-type peanut oneself at the base portion of the wild-type peanut seedling being used as stock grows, and growing way is far longer than Graft, fights for nutrition with Graft; so these budlets will be cut off at any time, thus add the workload of researchist.Moreover Graft surviving rate is not very high, only has about 60%.
Existing some investigators has obtained the substratum being conducive to peanut and taking root at present, as Chinese patent literature CN102577981A(application number 201210079420.4) disclose the transgenic peanuts tissue cultured seedling strong sprout that a kind of strong sprout is effective, rooting rate is high, quality of rooting is good, the method for taking root, comprise the steps: (1) by differentiation-inducing go out the peanut tissue culture seedlings of Multiple Buds transfer to strong seedling culture base A upper 20 day, within 10 days, change a subculture, the component of described strong seedling culture base A is MS substratum, and hormone used is phytokinin 6-BA; (2) tissue cultured seedling growing to more than 1.5cm is divided into individual plant, move to strong seedling culture base B upper 20 ~ 25 days, within 10 days, change a subculture, the composition of described strong seedling culture base B is MS substratum, and hormone used is the growth hormone NAA of phytokinin 6-BA and 0.1mg/L of 0.4mg/L; (3) treat that seedling grows to 2.5 ~ 4cm, move to root media and take root, the composition of described root media is 1/2MS substratum, and hormone used is growth hormone IBA and NAA, and concentration is respectively 3mg/L and 0.3mg/L.
Chinese patent literature CN101411305A(application number 200810219406.3) disclose a method of cultivating peanut Vitro Quick Reproduction, the method is cultivated on the basis with (4) root culture at following four steps (1) the explant surface sterilization included by existing tissue culture peanut in-vitro propagate, the generation of (2) evoking adventive bud, (3) Elongation of adventitious bud, is optimized improvement to the bud elongation medium in the indefinite bud generation substratum in step (2) and evoking adventive bud culture condition and step (3) and culture condition.The present invention adopts 4-forchlorfenuron as main component, promotes plant tissue generation indefinite bud, compared with the material occurred with existing promotion indefinite bud, activity is low, effect shows, and in indefinite bud generating process, explant there will not be black close to the tissue cultivating basal plane, promotes that blastogenesis is long.The inventive method compared with the method for existing promotion Elongation of adventitious bud, without the need to increasing specific equipment, Plant hormones regulators,gibberellins and cytokinin working concentration little, cost is low, extends to cultivate the short and effect of required time and show.
In technical scheme, the Multiple Buds of acquisition needs to cultivate for some time on strong seedling culture base or bud elongation medium, just can be used for taking root, be thus unfavorable for the fast seedling growing of peanut after seedling is grown up.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of peanut tissue culture seedlings Rapid Rooting substratum and cultural method are provided.
Terminological interpretation
IBA: indolebutyric acid;
NAA: naphthylacetic acid;
GA
3: Plant hormones regulators,gibberellins GA
3.
Technical solution of the present invention is as follows:
A kind of peanut tissue culture seedlings Rapid Rooting substratum, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.6 ~ 0.8mg, naphthylacetic acid (NAA) 0.08 ~ 0.12mg, Plant hormones regulators,gibberellins GA
3(GA
3) 0.8 ~ 1.2mg, inositol 80 ~ 120mg, vitamins B
1(VB
1) 0.8 ~ 1.2mg, pH5.8.
Preferred according to the present invention, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.65 ~ 0.75mg, naphthylacetic acid (NAA) 0.09 ~ 0.11mg, Plant hormones regulators,gibberellins GA
3(GA
3) 0.9 ~ 1.1mg, inositol 90 ~ 110mg, vitamins B
1(VB
1) 0.9 ~ 1.1mg, pH5.8.
MS minimum medium is that substratum is commonly used in this area, and component is as follows:
NH
4nO
31.65g/L, KNO
31.9g/L, CaCl
22H
2o0.44g/L, MgSO
47H
2o0.37g/L, KH
2pO
40.17g/L, KI0.83mg/L, H
3bO
36.2mg/L, MnSO
44H
2o22.3mg/L, ZnSO
47H
2o8.6mg/L, Na
2moO
42H
2o0.25mg/L, CuSO
45H
2o0.025mg/L, CoCl
26H
2o0.025mg/L, FeSO
27H
2o27.8mg/L, inositol 100mg/L, glycine 2mg/L, vitamin 0.1mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30g/L, agar 7.2g/L.
A kind of peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is cut 1.0 ~ 2.0 centimetres, is placed in peanut tissue culture seedlings Rapid Rooting substratum, at 25 DEG C, under 16 h light/8 h dark conditions, cultivate 20 ~ 30 days, namely bear a large amount of root system.
As indivedual tissue cultured seedling can not bear root system, only 1.0-1.5 centimetre, its tip need be cut, again take root.To the plantlet of transplant of better root system be had, normal management.Root system not too flourishing plant many growth for some time on root media can improve surviving rate greatly.
Preferred according to the present invention, the culturing step of described peanut tissue culture seedlings is as follows:
Peanut seed is placed in aseptic triangular flask, and first adding volume percent is 75% alcohol immersion 1 minute, takes out, immersing mass percent is the mercuric chloride solution of 0.1%, sterilizing 20 minutes, takes out, then with the residual mercuric chloride of aseptic water washing removing, remove seed coat, be placed in 1/2MS substratum, cultivate 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducement substratum and sprout, cultivate 20 ~ 30 days and get final product;
Preferred further according to the present invention, described bud inducement substratum, the basis of MS minimum medium is added following component:
Naphthylacetic acid (NAA) 0.7mg/L, 6-benzyl aminoadenine (6-BA) 8.0mg/L, pH5.8.
1/2MS substratum is that substratum is commonly used in this area, and component is as follows: except agar, and each component content is the half of MS minimum medium.
Beneficial effect
1, peanut tissue culture seedlings Rapid Rooting substratum of the present invention, the rooting rate of peanut tissue culture seedlings can be made to reach 100%, transplanting survival rate reaches more than 90%, solves the problem of peanut tissue culture seedling rooting difficulty, for the development of peanut tissue culture and genetic transformation is laid a good foundation.
2, in peanut tissue culture seedlings Rapid Rooting substratum of the present invention, additionally with the addition of VB
1and inositol, the conbined usage of these two kinds of VITAMIN, has larger promoter action for the Rapid Rooting of peanut Multiple Buds and plant strain growth.
3, the more existing peanut tissue culture method of peanut tissue culture seedlings Rapid Rooting cultural method of the present invention is compared, eliminate Multiple Buds extend or strong sprout this process, be directly that the Multiple Buds of 1.0-2.0 centimetre is positioned on root media and takes root by length, not only extending but also can strong sprout while taking root, formed with healthy and strong root system whole plant, substantially reduce the regeneration period of peanut Multiple Buds, simplify operation steps, save time, man power and material.
Accompanying drawing explanation
Fig. 1 is peanut tissue culture seedlings;
Fig. 2 is the peanut tissue culture seedlings photo of taking root;
Fig. 3 is the healthy and strong root system photo that in embodiment 1, peanut tissue culture seedlings bears;
Fig. 4 is the healthy and strong root system photo that in embodiment 2, peanut tissue culture seedlings bears;
Fig. 5 is the healthy and strong root system photo that in embodiment 3, peanut tissue culture seedlings bears;
Fig. 6 is the healthy and strong root system photo that in embodiment 4, peanut tissue culture seedlings bears;
Fig. 7 is the healthy and strong root system photo that in embodiment 5, peanut tissue culture seedlings bears;
Fig. 8 is the root system photo that in comparative example 1, peanut tissue culture seedlings bears;
Fig. 9 is the root system photo that in comparative example 2, peanut tissue culture seedlings bears;
Figure 10 is the root system photo that in comparative example 3, peanut tissue culture seedlings bears.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
Experiment material
Embodiment 1 ~ 3 adopts peanut varieties for ' flower educates 22 ', and embodiment 4 adopts peanut varieties to be ' rich spend No. 1 ', and the peanut varieties that embodiment 5 adopts is for ' Shandong spends 14 ', and above-mentioned kind is common commercially available kind.
Substratum:
MS minimum medium, component is as follows:
NH
4nO
31.65g/L, KNO
31.9g/L, CaCl
22H
2o0.44g/L, MgSO
47H
2o0.37g/L, KH
2pO
40.17g/L, KI0.83mg/L, H
3bO
36.2mg/L, MnSO
44H
2o22.3mg/L, ZnSO
47H
2o8.6mg/L, Na
2moO
42H
2o0.25mg/L, CuSO
45H
2o0.025mg/L, CoCl
26H
2o0.025mg/L, FeSO
27H
2o27.8mg/L, inositol 100mg/L, glycine 2mg/L, vitamin 0.1mg/L, pyridoxine hydrochloride 0.5mg/L, nicotinic acid 0.5mg/L, sucrose 30g/L, agar 7.2g/L.
1/2MS substratum, component is as follows: except agar, and each component content is the half of MS minimum medium.
Bud inducement substratum, the basis of MS minimum medium is added following component:
Naphthylacetic acid (NAA) 0.7mg/L, 6-benzyl aminoadenine (6-BA) 8.0mg/L, pH5.8.
Embodiment 1
A kind of peanut tissue culture seedlings Rapid Rooting substratum, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.7mg/L, naphthylacetic acid (NAA) 0.1mg/L, Plant hormones regulators,gibberellins GA
3(GA
3) 1.0mg/L, inositol 100mg/L, vitamins B
1(VB
1) 1.0mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings:
That selects size uniformity ' spends the seed of educating 22 ' to be placed in aseptic triangular flask, first adding volume percent is 75% alcohol immersion 1 minute, take out, immersing mass percent is 0.1% mercuric chloride solution, rock continuously, sterilizing 20 minutes, take out, with aseptic water washing 4 times, wash away residual mercuric chloride, then the peanut seed after sterilizing is removed seed coat, being placed in 1/2MS substratum cultivates after 5 days, takes out, cuts hypocotyl, be placed in induced bundle on bud inducement substratum to sprout, after 20 ~ 30 days, obtain healthy and strong tissue cultured seedling (as shown in Figure 1);
A kind of peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut 1.0 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting substratum, in 25 DEG C, cultivate under 16 h light/8 h dark conditions and within 7 days, can see that cutting part starts to expand and has the tip of a root to be formed, cultivate and within 25 days, namely have a large amount of normal root to generate, and on root, also can constantly have side root to generate (as shown in Figure 2 and Figure 3).Be transplanted in flowerpot, normal management.
After testing, survival rate 97%.
Embodiment 2
A kind of peanut tissue culture seedlings Rapid Rooting substratum, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.6mg/L, naphthylacetic acid (NAA) 0.08mg/L, Plant hormones regulators,gibberellins GA
3(GA
3) 0.8mg/L, inositol 80mg/L, vitamins B
1(VB
1) 0.8mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings as described in Example 1.
A kind of peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut 2.0 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting substratum, in 25 DEG C, cultivate under 16 h light/8 h dark conditions and within 10 days, can see that cutting part starts to expand and has the tip of a root to be formed, cultivate and within 30 days, namely have a large amount of normal root to generate, and on root, also can constantly have side root to generate (as shown in Figure 4).Be transplanted in flowerpot, normal management.
After testing, survival rate 94%.
Embodiment 3
A kind of peanut tissue culture seedlings Rapid Rooting substratum, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.75mg/L, naphthylacetic acid (NAA) 0.11mg/L, Plant hormones regulators,gibberellins GA
3(GA
3) 1.1mg/L, inositol 110mg/L, vitamins B
1(VB
1) 1.1mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings as described in Example 1.
A kind of peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut 1.5 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting substratum, in 25 DEG C, cultivate under 16 h light/8 h dark conditions and within 8 days, can see that cutting part starts to expand and has the tip of a root to be formed, cultivate and within 20 days, namely have a large amount of normal root to generate, and on root, also can constantly have side root to generate (as shown in Figure 5).Be transplanted in flowerpot, normal management.
After testing, survival rate 95%.
Embodiment 4
A kind of peanut tissue culture seedlings Rapid Rooting substratum, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.7mg/L, naphthylacetic acid (NAA) 0.1mg/L, Plant hormones regulators,gibberellins GA
3(GA
3) 1.0mg/L, inositol 100mg/L, vitamins B
1(VB
1) 1.0mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings:
The seed selecting ' rich spend No. 1 ' of size uniformity is placed in aseptic triangular flask, first adding volume percent is 75% alcohol immersion 1 minute, take out, adding mass percent is 0.1% mercuric chloride solution, rock continuously, sterilizing 20 minutes, take out, with aseptic water washing 4 times, wash away residual mercuric chloride, then the peanut seed after sterilizing is removed seed coat, being placed in 1/2MS substratum cultivates after 5 days, takes out, cuts hypocotyl, be placed in induced bundle on bud inducement substratum to sprout, after 25 days, obtain healthy and strong tissue cultured seedling.
A kind of peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut 1.5 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting substratum, in 25 DEG C, cultivate under 16 h light/8 h dark conditions and within 9 days, can see that cutting part starts to expand and has the tip of a root to be formed, cultivate and within 25 days, namely have a large amount of normal root to generate, and on root, also can constantly have side root to generate (as shown in Figure 6).Be transplanted in flowerpot, normal management.
After testing, survival rate 96%.
Embodiment 5
A kind of peanut tissue culture seedlings Rapid Rooting substratum, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.6mg/L, naphthylacetic acid (NAA) 0.12mg/L, Plant hormones regulators,gibberellins GA
3(GA
3) 1.2mg/L, inositol 80mg/L, vitamins B
1(VB
1) 0.8mg/L, pH5.8.
The acquisition of peanut tissue culture seedlings:
Select size uniformity ' Shandong spends the seed of 14 ' to be placed in aseptic triangular flask, and first adding volume percent is 75% alcohol immersion 1 minute, take out, adding mass percent is 0.1% mercuric chloride solution, rocks continuously, sterilizing 20 minutes, take out, with aseptic water washing 4 times, wash away residual mercuric chloride, then the peanut seed after sterilizing is removed seed coat, being placed in 1/2MS substratum cultivates after 5 days, takes out, cuts hypocotyl, be placed in induced bundle on bud inducement substratum to sprout, after 30 days, obtain healthy and strong tissue cultured seedling.
A kind of peanut tissue culture seedlings Rapid Rooting cultural method, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is neatly cut 1.5 centimetres, be placed in above-mentioned peanut tissue culture seedlings Rapid Rooting substratum, in 25 DEG C, cultivate under 16 h light/8 h dark conditions and within 7 days, can see that cutting part starts to expand and has the tip of a root to be formed, cultivate and within 25 days, namely have a large amount of normal root to generate, and on root, also can constantly have side root to generate (as shown in Figure 7).Be transplanted in flowerpot, normal management.
After testing, survival rate 95%.
Comparative example 1
Root media, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.7mg/L, naphthylacetic acid (NAA) 0.1mg/L, Plant hormones regulators,gibberellins GA3(GA3) 1.0mg/L, VITMAIN B1 (VB1) 1.0mg/L, pH5.8.
Other experimental techniques are with embodiment 1.
Cultivate after 15 days, most of Multiple Buds can bear root system, and average each bastem portion incision approximately generates 5 ~ 6 main roots.When continuing to be cultured to 25 days, main root approximately grows to 4 ~ 5 centimetres, only bears 4 ~ 5 little side roots, be transplanted in flowerpot, normal management above every root main root.
After testing, surviving rate is 85%.
Comparative example 2
Root media, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.7mg/L, naphthylacetic acid (NAA) 0.1mg/L, Plant hormones regulators,gibberellins GA3(GA3) 1.0mg/L, inositol 100mg/L, sucrose 20g/L, pH5.8.
Other experimental techniques are with embodiment 1.
Cultivate after 15 days, most of Multiple Buds can bear root system, and average each bastem portion incision approximately generates 5 ~ 6 main roots.When continuing to be cultured to 25 days, main root approximately grows to 4 ~ 5 centimetres, only bears 5 ~ 6 little side roots, plant in flowerpot, normal management above every root main root.
After testing, surviving rate is 81%.
Comparative example 3
Root media, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid (IBA) 0.7mg/L, naphthylacetic acid (NAA) 0.1mg/L, Plant hormones regulators,gibberellins GA3(GA3) 1.0mg/L, sucrose 20g/L, pH5.8.
Other experimental techniques are with embodiment 1.
Cultivate after 15 days, most of Multiple Buds can bear root system, and average each bastem portion incision approximately generates 3 ~ 4 main roots.When continuing to be cultured to 25 days, main root approximately grows to 3 ~ 4 centimetres, only bears 3 ~ 4 little side roots, be transplanted in flowerpot, normal management above every root main root.
After testing, surviving rate is 73%.
Interpretation of result:
Show through embodiment interpretation of result, the rooting efficiency of this prescription of rooting medium is not by the impact of peanut genotype, and the tissue cultured seedling of whichever peanut varieties, this root media can comparatively fast grow healthy and strong root system.
Show through comparative example interpretation of result, interpolation VITMAIN B1 and inositol can significantly improve the ratio of taking root of peanut tissue culture seedlings simultaneously, and the side root that main root bears is more.
Claims (2)
1. a peanut tissue culture seedlings Rapid Rooting substratum, is characterized in that, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid 0.6 ~ 0.8 mg, naphthylacetic acid 0.08 ~ 0.12 mg, Plant hormones regulators,gibberellins GA
30.8 ~ 1.2 mg, inositol 80 ~ 120 mg, vitamins B
10.8 ~ 1.2 mg, pH5.8.
2. peanut tissue culture seedlings Rapid Rooting substratum as claimed in claim 1, is characterized in that, the basis of often liter of MS minimum medium is added following component:
Indolebutyric acid 0.65 ~ 0.75 mg, naphthylacetic acid 0.09 ~ 0.11 mg, Plant hormones regulators,gibberellins GA
30.9 ~ 1.1 mg, inositol 90 ~ 110 mg, vitamins B
10.9 ~ 1.1 mg, pH5.8.
3
.peanut tissue culture seedlings Rapid Rooting substratum as claimed in claim 1, it is characterized in that, MS minimum medium component is as follows:
NH
4nO
31.65 g/L, KNO
31.9 g/L, CaCl
22H
2o 0.44 g/L, MgSO
47H
2o 0.37 g/L, KH
2pO
40.17 g/L, KI 0.83 mg/L, H
3bO
36.2 mg/L, MnSO
44H
2o 22.3 mg/L, ZnSO
47H
2o 8.6 mg/L, Na
2moO
42H
2o 0.25 mg/L, CuSO
45H
2o 0.025 mg/L, CoCl
26H
2o 0.025 mg/L, FeSO
27H
2o 27.8 mg/L, inositol 100 mg/L, glycine 2 mg/L, vitamin 0.1 mg/L, pyridoxine hydrochloride 0.5 mg/L, nicotinic acid 0.5 mg/L, sucrose 20 g/L, agar 7.2 g/L.
4
.a kind of peanut tissue culture seedlings Rapid Rooting cultural method, it is characterized in that, step is as follows:
The young tender shoots tip of peanut tissue culture seedlings is cut 1.0 ~ 2.0 centimetres, is placed in peanut tissue culture seedlings Rapid Rooting substratum according to claim 1, at 25 DEG C, under 16 h light/8 h dark conditions, cultivate 20 ~ 30 days, transplant, normal management.
5
.method as claimed in claim 4, is characterized in that, the culturing step of described peanut tissue culture seedlings is as follows:
Peanut seed is placed in aseptic triangular flask, and first adding volume percent is 75% alcohol immersion 1 minute, takes out, immersing mass percent is the mercuric chloride solution of 0.1%, sterilizing 20 minutes, takes out, then with the residual mercuric chloride of aseptic water washing removing, remove seed coat, be placed in 1/2 MS substratum, cultivate 5 days, take out, cut hypocotyl, be placed in induced bundle on bud inducement substratum and sprout, cultivate 20 ~ 30 days and get final product.
6
.method as claimed in claim 5, is characterized in that, described bud inducement substratum is: following component is added on the basis of MS minimum medium:
Naphthylacetic acid 0.7 mg/L, 6-benzyl aminoadenine 8.0 mg/L, pH5.8.
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| CN105494415A (en) * | 2016-01-19 | 2016-04-20 | 耿云强 | Rooting agent suitable for various plants and preparation method thereof |
| CN109809896A (en) * | 2017-11-22 | 2019-05-28 | 丹阳市陵口镇城墅蔬菜专业合作社 | Compost is used in a kind of plantation of crowndaisy chrysanthemum |
| CN110367124B (en) * | 2019-08-29 | 2021-04-09 | 淮北师范大学 | Method for constructing peanut cotyledon regeneration system |
| CN110786245A (en) * | 2019-12-16 | 2020-02-14 | 山东省花生研究所 | Peanut tissue culture seedling root induction culture medium and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217541A (en) * | 2011-05-23 | 2011-10-19 | 山东省花生研究所 | Peanut rooting medium formula |
| CN102577981A (en) * | 2012-03-23 | 2012-07-18 | 山东省花生研究所 | Method for strengthening and rooting tissue culture seedlings of transgenic peanuts |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217541A (en) * | 2011-05-23 | 2011-10-19 | 山东省花生研究所 | Peanut rooting medium formula |
| CN102577981A (en) * | 2012-03-23 | 2012-07-18 | 山东省花生研究所 | Method for strengthening and rooting tissue culture seedlings of transgenic peanuts |
Non-Patent Citations (1)
| Title |
|---|
| 植物组培技术在花生育种方面的应用;翁跃进;《中国油料作物学报》;19890330(第01期);第88页倒数第1-2行 * |
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