CN103642684A - Magnetic probe search type cell sorting device - Google Patents
Magnetic probe search type cell sorting device Download PDFInfo
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- CN103642684A CN103642684A CN201310688961.1A CN201310688961A CN103642684A CN 103642684 A CN103642684 A CN 103642684A CN 201310688961 A CN201310688961 A CN 201310688961A CN 103642684 A CN103642684 A CN 103642684A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention relates to a magnetic probe search type cell sorting device. The sorting device is composed of a gantry type XYZ three-axis mechanical arm device, a mechanical arm digital controller, an engine base, a machine body, a magnetic probe, a probe fixture, a test board, a test box and a release magnet body, wherein the magnetic probe is connected to the probe fixture, the probe fixture is arranged on the gantry type XYZ three-axis mechanical arm device, the mechanical arm digital controller is connected with the gantry type XYZ three-axis mechanical arm device, the gantry type XYZ three-axis mechanical arm device is arranged above the test box, the test box is arranged on the table top of the test board, the test board is arranged on the machine body, the machine body is arranged above the engine base, and the release magnet body is arranged in the machine body and is located below the test box; sample liquid is filled in the test box, the magnetic probe is arranged on the probe fixture, the probe fixture can move in the gantry type XYZ three-axis mechanical arm device, the magnetic probe sucks and captures a target cell in the test box, and the release magnet body sucks the target cell to recycle and collect the target cell on the magnetic probe.
Description
Technical field
The present invention relates to a kind of magnetic probe search type cell sorting device, particularly relate to the highly sensitive automatic biological cell sorting technology in biomass cells sorting field, belong to biomass cells size separation equipment.
Background technology
In medical diagnosis on disease, the fields such as drug test, researchist need to extract technology and the equipment of specific cells from mixed the sample of various tissue of biological cells, to obtain pure cell sample, does various experimental analyses and diagnosis.Obtain higher sensitivity, higher separation velocity and purity, better sample type adaptive faculty, more efficient cell sorting technology and equipment automatically, is the target of effort of cell sorting field.
Existing cell sorting equipment mainly contains:
One, centrifugal classification technology, for the greatly different in size cell of sorting, is more used for sorting cells device.By differential centrifugation, can, by the preliminary sorting of organoid, often need further pass through the centrifugal row again of density level bands sorting purifying.Velocity sedimentation technology is a kind of in centrifugal classification technology, the cell differing in size for separating density is close or organoid.This to fall the Media density that method adopts lower, and the maximum density Should of medium is less than the minimum density that is sorted biological particles.It utilizes biological particles, comprises that cell or thin device reach sorting by settling ratio separately in very mild density gradient medium with different velocity sedimentations.This method can sorting cell very limited, and huge centrifugal force and process for a long time all unfavorable to cell.Maxicell is more subject to the damage of high centrifugal force than minicell, and the cell resting in isodensity medium is subject to larger damage than the cell being in moving.Therefore, this method is suitable for sorting cells device, and is not too suitable for sorting and purifying cells.
Two, fluidic cell sorting technology art is individual cells to be carried out to a special kind of skill of fast quantitative analysis and sorting.In analysis or assorting room, wrap in the nozzle that the cell in sheath fluid is controlled by HF oscillation, the drop that formation comprises individual cells, under the irradiation of laser beam, these cells send scattered light and fluorescence, detect Turn be changed to electrical signal through detector, send into computer and process, output statistics, and can sub-elect highly purified cell subsets according to these character.Though this method purity and the rate of recovery are high, apparatus expensive, technical sophistication, needs professional and technical personnel to operate, during operational cost.Because there are the parts of reusing that sample must contact, can not avoid sample crossed contamination completely.In addition, this method requires the treated sample number of cells that hits can not be less than hundreds of.Not good to the sample separation results that target cell content is few.In addition, it is also not suitable for the application that cytoactive is had relatively high expectations.
Three, cell electrophoresis technology.With clean plus or minus electric charge, can there is swimming in cell surface under certain pH value under the effect of extra electric field, and this phenomenon is called cell electrophoresis.Various cells or different in the allogenic cell carrying capacity of different physiological statuss, therefore the mobility speed in certain electric field is different.Under constant current field condition, homocellular electrophoretic velocity quite stable, thereby can come some kind cell position of sorting by measuring electrophoretic velocity, therefore on diagnosing the illness, there is certain values.The shortcoming of this sorting technology is that cytoactive is had to damage, is difficult to the cell that sorting kind is close
Four, immunological magnetic bead sorting technology.Immunomagnetic beads method sorting cells is can combine with the specific monoclonal antibody that is connected with magnetic bead based on cell-surface antigens, in externally-applied magnetic field, the cell being connected with magnetic bead by antibody is adsorbed and is trapped in magnetic field, without the cell of this kind of surface antigen owing to not being combined and thering is no magnetic with the specific monoclonal antibody that is connecting magnetic bead, in magnetic field, do not stop, thereby make cell be able to sorting.This technology is strong to the resolving power of target cell, and without expensive large-scale instrument, equipment is relatively cheap, and method is simple, is a kind of very potential method.But existing size separation equipment efficiency is low, and consumptive material is more expensive, very responsive to the impurity being sorted in sample.Greatly limited its practical function.
Adopt at present the equipment of this technology to mainly contain following several:
1, magnetic cell sorting instrument, comes from German Mei Tian Ni biotech company (MiltenyiBiotec), and it adopts the design of magnetic pipeline streaming, the less magnetic bead of general use.Very effective on this theoretical method, but because be sorted, in sample, be difficult to avoid oarse-grained biological tissue in actual use, cause the stifled match of pipeline or target cell (10) to lose, so the index that the cell sorting reliability that can reach in practicality and sensitivity and producer declare has suitable distance.
2, magnetic bead catches instrument (BeadRetriever), comes from U.S. Dynal company, and it adopts the design of magnetic post test tube type, the larger magnetic bead of general use.This method can be avoided the problem of line clogging, but sensitivity is not enough, and cell capture rate and purity are lower, and practical effect is also undesirable.
3, cell searching instrument (CallSearch), is the Veridex of subsidiary corporation of Johnson Co., and LLC develops recently, also adopts immunological magnetic bead sorting primary reason.Now, by U.S. FDA access, be applied to progresson free survival rate and total survival rate that prediction has metastatic mammary cancer, colorectal cancer or prostate cancer.This equipment has certain effect to cancer diagnosis.But the cell sub-electing loses activity, this has limited its range of application.
Summary of the invention
The object of the invention is to solve above-mentioned existing technological deficiency, provides a kind of better target cell capture rate high, adaptable to sample, easy and simple to handle, the magnetic probe search type cell sorting device (claiming again CI cell searching instrument) that level of automation is high.
Technical solution of the present invention is: magnetic probe search type cell sorting device is by planer-type XYZ three axle robert device (1), mechanical manipulator digitial controller (2), support (3), body (4), magnetic probe (5), probe jig (51), test board (6), testing cassete (7) and discharge magnet (8) formation
Described magnetic probe (5) is connected on probe jig (51), probe jig (51) is placed on planer-type XYZ three axle robert device (1), mechanical manipulator digitial controller (2) connects planer-type XYZ three axle robert device (1), planer-type XYZ three axle robert device (1) is placed in testing cassete (7) top, testing cassete (7) is placed on the table top of test board (6), test board (6) is placed on body (4), body (4) is placed in support (3) top, discharges the below that magnet (8) is placed in body (4) and is positioned at testing cassete (7);
Wherein: testing cassete is equipped with sample liquid in (7), magnetic probe (5) is installed on probe jig (51), probe jig (51) can be in planer-type XYZ three axle robert device (1) movement, magnetic probe (5) testing cassete (7) inner complete the suction of target cell (10) is caught after, by discharging the suction effect of magnet (8) to target cell (10), complete the recovery of the target cell (10) on magnetic probe (5) is collected.
Described planer-type XYZ three axle robert device (1) comprising: X-axis servomotor (11) connects X-axis beam guideway (12), and Y-axis servomotor (13) connects the two guide rails (14) of Y-axis planer-type, and Z axis servomotor (15) connects Z axis slide block (16),
Wherein: magnetic probe (5) connects Z axis slide block (16), it is upper that Z axis slide block (16) is fixed on probe jig (51), and Z axis servomotor (15) control magnetic probe (5) prolongs Z axis and slides up and down on Z axis slide block (16);
It is upper that described probe jig (51) is placed in X-axis beam guideway (12), and under the effect of X-axis servomotor (11), probe jig (51) moves horizontally on X-axis beam guideway (12);
It is upper that described X-axis beam guideway (12) is fixed on the two guide rails (14) of Y-axis planer-type, and X-axis beam guideway (12) moves horizontally under the effect of Y-axis servomotor (13) on the two guide rails (14) of Y-axis planer-type;
Described mechanical manipulator digitial controller (2) connects X-axis servomotor (11), Y-axis servomotor (13) and Z axis servomotor (15), for controlling magnetic probe (5) in the inner movement of body (4).
Described testing cassete (7) is provided with three containers, be respectively sampling receptacle (71), clean container (72) and target cell (10) and collect container (73), sample liquid is housed in sampling receptacle (71), clean container is equipped with scavenging solution in (72), magnetic probe (5) stretches in sampling receptacle (71) and regains after collection adsorption target cell (10), move to again to extend in clean container (72) and clean DC thing (101), finally in target cell (10) is collected container (73), target cell (10) is discharged.
Described release magnet (8) is placed in body (4) inside, be positioned at target cell (10) collect container (73) under.
5. according to a kind of magnetic probe search type cell sorting device described in any one in the claims, it is characterized in that described magnetic probe (5) end also comprises an isolation outer cover (52) being set with removably, the cover mouth edge of described isolation outer cover (52) has the outward extending limit (53) of prolonging of a circle.
Also comprise an isolation outer cover (52) handling frame, described isolation outer cover (52) handling are placed on testing cassete (7) side or are installed on the edge of body (4), isolation outer cover (52) handling mount and comprise encapsulation parts and dismounting parts, encapsulation parts and dismounting parts all have a probe draw-in groove (91) that holds magnetic probe (5), the upper end place of probe draw-in groove (91) has the baffle plate (92) that prolongs limit (53) internal diameter that a spacing is greater than isolation outer cover (52) internal diameter, is less than isolation outer cover (52)
Isolation outer cover (52) can be positioned in the draw-in groove of encapsulation parts, and shelves are blocked on baffle plate (92) in the limit (53) of prolonging of isolation outer cover (52);
Stretch in the draw-in groove of dismantling parts with the magnetic probe of isolating outer cover (52) after, up move, the limit (53) of prolonging of isolation outer cover (52) is stopped by baffle plate (92), completes the dismounting of isolation outer cover (52).
Also comprise a discarded isolation outer cover disposable box (54), described discarded isolation outer cover disposable box (54) is placed under isolation outer cover handling framves (9).
Described magnetic probe (5) is changeable type probe, and magnetic probe (5) is permanent magnetism probe or electromagnetic probe.
Described (7) three containers of testing cassete: sampling receptacle (71), clean container (72) and target cell (10) are collected container (73), have at least two groups to form side by side, and meanwhile, the quantity of magnetic probe (5) and the quantity of groups of containers are consistent.
Described sample liquid is blood, marrow, body fluid or the liquid that contains viable cell.
The present invention is based on immune antibody magnetic bead cellular segregation principle, adopt unsteady magnetic probe and the cell harvesting access panel of unique design, under the operation of computer controller, efficiently complete by the search of immunomagnetic beads labeled cell and acquisition procedure fast automaticly, successfully realize the cell separation apparatus with highly sensitive height and high reliability.
Principle of work: adopt planer-type XYZ three axle robert device (1) operating magnetic probe (5) to be inserted in the liquid sample that contains target cell (10) and realize target cell (10) sorting as searching movement.The liquid sample being sorted is first done immunomagnetic beads mark, then inject in the sampling receptacle (71) of testing cassete (7), when thering is the close target cell (10) being labeled of probe of strong magnetic, target cell (10) can be adsorbed to detecting head surface, the three coordinate robotic manipulation that magnetic probe (5) is controlled by mechanical manipulator digitial controller (2), by the default whole sample of track mobile search, after having searched for, magnetic probe (5) moves to and in clean container (72), washes the dirt of being infected with outside target cell (10), moving to the release magnet (8) by reverse magnetic force in target cell (10) collection container (73) is released into the target cell being adsorbed (10) in target cell (10) collection container (73) from magnetic probe (5) again.Magnetic probe (5) appearance cover has the non magnetic disposable use isolation outer cover (52) that can isolate sample and probe body to avoid magnetic probe (5) and sample contaminated.
The present invention realizes automated cell sorting by following steps:
One, the sampling receptacle (71) of testing cassete (7) will be put into by the sample liquids of immunomagnetic beads mark, in clean container (72), put into cell scavenging solution, in collecting container, put into cell-preservation liquid, then testing cassete (7) is put into the test board (6) of apparatus of the present invention.
Two, magnetic probe (5) moves to isolation outer cover (52) handling frame under the operation of planer-type XYZ three axle robert device (1), from encapsulation parts top, aim at after the isolation outer cover (52) of probe draw-in groove (91) and decline, stretch in isolation outer cover (52), after suit, stretch back, complete automatic sleeving isolation outer cover (52).
Three, magnetic probe (5) is under the operation of planer-type XYZ three axle robert device (1) in insertion sampling receptacle (71), and by along circular arc, spiral and straight path with the search target cell (10) in the XX speed sampling receptacle (71) of setting.
Four, after having searched, under the operation of planer-type XYZ three axle robert device (1), magnetic probe (5) inserts clean container (72), along default track and speed, make washing motion, to remove other contaminants (101) outside the target cell (10) that is attached to isolation outer cover (52) outside surface.
Five, after having cleaned, under the operation of planer-type XYZ three axle robert device (1), isolation outer cover (52) is made suitable part lock out operation with magnetic probe (5), increase magnetic probe (5) to the spacing of isolation outer cover (52) surface targets cell (10), to reduce the suction of magnetic probe (5) to target cell (10); Again magnetic probe (5) insertion target cell (10) is collected in container (73) and moved along default track and speed, by being arranged on release magnet (8) effect of target cell (10) collection container (73) below, target cell (10) absorption that is attached to isolation outer cover (52) outside surface is released into collection container.
Six, after having discharged, under the operation of planer-type XYZ three axle robert device (1), magnetic probe (5) enters the dismounting parts place in isolation outer cover (52) handling frame, stretch in the draw-in groove of dismantling parts with the magnetic probe (5) of isolating outer cover (52) after, up move, the limit (53) of prolonging of isolation outer cover (52) is stopped by baffle plate (92), completes the dismounting of isolation outer cover (52), isolation outer cover (52) and magnetic probe (5) sorting completely.
Above step realizes the assorting room of target cell (10).Actual measurement shows, the present invention can obtain high cell sorting purity, and has high cellular resolution, can from billions of cells, sub-elect fast a unique target cell (10).Therefore the present invention has great using value.
Apparatus of the present invention have obtained the multinomial advantage that existing cell separation technology equipment does not possess, comprise easy and simple to handle, separated fast, use cost is cheap etc.
Accompanying drawing explanation
Fig. 1: magnetic probe search type cell sorting device schematic diagram.
Fig. 2: testing cassete structural representation.
Fig. 3: magnetic probe structural representation.
Fig. 4 and isolation outer cover scheme of installation.
Fig. 5: load isolation outer cover schematic diagram.
Fig. 6: separated isolation outer cover schematic diagram.
Fig. 7: acquisition target cell schematic diagram in sampling receptacle.
Fig. 8: clean target cell schematic diagram in clean container.
Fig. 9: target cell is collected in container and discharges target cell schematic diagram.
Figure 10: target cell capture rate statistics figure.
Figure 11: target cell Photomicrograph is hunted down.
Figure 12: magnetic probe search type cell sorting device for carrying out said structure front view.
Figure 13: magnetic probe search type cell sorting device for carrying out said structure side view.
Wherein: 1-planer-type XYZ three axle robert device, 11-X-axis servomotor, 12-X-axis beam guideway, 13-Y-axis servomotor, the two guide rails of 14-Y-axis planer-type, 15-Z axis servomotor, 16-Z axis slide block,
2-mechanical manipulator digitial controller, 3-support, 4-body,
5-magnetic probe, 51-probe jig, 52-isolation outer cover, 53-prolong limit, 54-discarded isolation outer cover disposable box,
6-test board,
7-testing cassete, 71-sampling receptacle, 72-clean container, 73-target cell is collected container,
8-release magnet,
9-isolation outer cover handling frame, 91-probe draw-in groove, 92-baffle plate,
10-target cell, 101-contaminant.
Embodiment
Sorting unit of the present invention is shown in Fig. 1, Figure 11 and Figure 12.Comprise by planer-type XYZ three axle robert device 1, mechanical manipulator digitial controller 2, support 3, body 4, magnetic probe 5, probe jig 51, test board 6, testing cassete 7 and discharge magnet 8 forming,
Wherein: magnetic probe 5 connects Z axis slide block 16, Z axis slide block 16 is fixed on probe jig 51, and Z axis servomotor 15 control magnetic probes 5 prolong Z axis and slide up and down on Z axis slide block 16;
Described probe jig 51 is placed on X-axis beam guideway 12, and under the effect of X-axis servomotor 11, probe jig 51 moves horizontally on X-axis beam guideway 12;
Described X-axis beam guideway 12 is fixed on the two guide rails 14 of Y-axis planer-type, and X-axis beam guideway 12 moves horizontally under the effect of Y-axis servomotor 13 on the two guide rails 14 of Y-axis planer-type;
Described mechanical manipulator digitial controller 2 connects X-axis servomotor 11, Y-axis servomotor 13 and Z axis servomotor 15, for controlling magnetic probe 5 in the movement of body 4 inside.
Automatic sorting process starts by loading isolation outer cover 52, mechanical manipulator moves to magnetic probe 5 directly over the cleaning isolation outer cover 52 that is placed in isolation outer cover handling frame 9, accurately magnetic probe 5 is inserted to isolation outer cover 52, isolation outer cover 52 endoporus and the tight fit of magnetic probe 5, magnetic probe 5 moves up isolation outer cover 52 is pulled away to isolation outer cover handling frame 9 in the lump, and it is complete that isolation outer cover 52 loads step.See Fig. 5.
Mechanical manipulator moves to sampling receptacle 71 tops by the magnetic probe 5 that isolation outer cover 52 is housed, slowly insert and be equipped with in the sampling receptacle 71 of sample solution, then with 2 mm/second along continuous straight runs, move magnetic probe 5, magnetic probe 5 motion tracks cover whole sampling receptacle 71 surface-area.Magnetic probe 5 translational speeds can be by revising mechanical manipulator digitial controller 2 parameter changes.In slow moving process, the powerful magnetic field of magnetic probe 5 ends is adsorbed onto isolation outer cover 52 surfaces by near target cell 10.Wait to search complete, magnetic probe 5 slowly moves up and leaves sample solution.Now isolate outer cover 52 surfaces and have captive target cell 10 and a small amount of contaminant 101.See Fig. 6.
In order to improve the purity of sorting cells, next need to carry out cleaning step: mechanical manipulator moves to clean container 72 tops by probe, slowly inserts cleaning solution, then with the speed along continuous straight runs of 4 ~ 10 mm/second, make washing motion.Magnetic probe 5 translational speeds are slightly faster than catching step, to reach the effect of the contaminant 101 that cleans non-target cell 10.Cleaning translational speed also can be by revising mechanical manipulator digitial controller 2 parameter changes.In moving process, firmly adsorb target cell 10 in the powerful magnetic field of magnetic probe 5 ends, by the contaminant 101 of marked by magnetic bead, is not subject to washing away of scavenging solution and departs from isolation outer cover 52 surfaces.Cleaning process finished after two minutes, and magnetic probe 5 again slowly moves up and leaves cleaning solution.A small amount of contaminant 101 of now isolating outer cover 52 surfaces has been removed.See Fig. 7.
Next just can discharge captive target cell 10 and collect container 73 to target cell 10.For the target cell 10 that is adsorbed on isolation outer cover 52 surfaces being discharged, in this device, mechanical manipulator will move to magnetic probe 5 to isolate on outer cover 52 handling framves and in a probe draw-in groove 91, will isolate outer cover 52 work part separation, to reduce the magnetic attracting force of probe to target cell 10.Then, mechanical manipulator moves to target cell 10 by magnetic probe 5 and collects container 73 tops, inserts in the cell culture fluid in target cell 10 collection containers 73.Under target cell 10 collection containers, powerful magnet is installed and discharges magnet 8, the target cell 10 that 8 pairs of magnets of release are adsorbed on isolation outer cover 52 surfaces has stronger magnetism.Target cell 10, under the effect of magnetic force that discharges magnet 8, departs from isolation outer cover 52, sinks to collection container bottom.Dispose procedure finished after one minute, and magnetic probe 5 moves up and leaves collection container, sees Fig. 8.
For avoiding sample crossed contamination, 52 of outer covers of isolation make a one-time use only, after target cell in a sample 10 is discharged, the probe draw-in groove 91 that in this device, mechanical manipulator will move to magnetic probe 5 on the dismounting parts on isolation outer cover 52 handling framves is interior completely separated to isolation outer cover 52 works, stretch in the draw-in groove of dismantling parts with the magnetic probe 5 of isolating outer cover 52 after, up move, the limit 53 of prolonging of isolation outer cover 52 is stopped by baffle plate 92, completes the dismounting of isolation outer cover 52.Isolation outer cover 52 after separation falls into isolation outer cover 52 and collects regularly removing of box wait.See Fig. 5.
Robot actions has been popped one's head in after above step and has been automatically reset, and completes with sound prompt operator cell sorting process, can take out 7 pairs of target cell 10 processing for further analysis of testing cassete.
By the actual test of on this device, the former blood that contains human circulation tumour cell CTC being done, show, the inventive method can reach more than 90% capture rate, sees Fig. 9.Within whole minute, selection operation required time is less than 30 minutes.Further experiment also shows, this contrive equipment has in containing billions of former blood that mixes cell catches the sensitivity of a unique circulating tumor cell, and is not subject to the interference of the impurity such as macrobead.The cytoactive that is hunted down is good, still has division and proliferation ability, sees Figure 10.
This device and existing cell sorting method contrast described in background technology, have target cell 10 capture rates high, adaptable to sample, easy and simple to handle, the remarkable advantage that level of automation is high, to cancer diagnosis, drug test and biomass cells correlative study have obvious practical value.
Claims (10)
1. a magnetic probe search type cell sorting device, it is characterized in that by planer-type XYZ three axle robert device (1), mechanical manipulator digitial controller (2), support (3), body (4), magnetic probe (5), probe jig (51), test board (6), testing cassete (7) and discharge magnet (8) forming
Described magnetic probe (5) is connected on probe jig (51), probe jig (51) is placed on planer-type XYZ three axle robert device (1), mechanical manipulator digitial controller (2) connects planer-type XYZ three axle robert device (1), planer-type XYZ three axle robert device (1) is placed in testing cassete (7) top, testing cassete (7) is placed on the table top of test board (6), test board (6) is placed on body (4), body (4) is placed in support (3) top, discharges the below that magnet (8) is placed in body (4) and is positioned at testing cassete (7);
Wherein: testing cassete is equipped with sample liquid in (7), magnetic probe (5) is installed on probe jig (51), probe jig (51) can be in planer-type XYZ three axle robert device (1) movement, magnetic probe (5) testing cassete (7) inner complete the suction of target cell (10) is caught after, by discharging the suction effect of magnet (8) to target cell (10), complete the recovery of the target cell (10) on magnetic probe (5) is collected.
2. a kind of magnetic probe search type cell sorting device according to claim 1, it is characterized in that described planer-type XYZ three axle robert device (1) comprising: X-axis servomotor (11) connects X-axis beam guideway (12), Y-axis servomotor (13) connects the two guide rails (14) of Y-axis planer-type, Z axis servomotor (15) connects Z axis slide block (16)
Wherein: magnetic probe (5) connects Z axis slide block (16), it is upper that Z axis slide block (16) is fixed on probe jig (51), and Z axis servomotor (15) control magnetic probe (5) prolongs Z axis and slides up and down on Z axis slide block (16);
It is upper that described probe jig (51) is placed in X-axis beam guideway (12), and under the effect of X-axis servomotor (11), probe jig (51) moves horizontally on X-axis beam guideway (12);
It is upper that described X-axis beam guideway (12) is fixed on the two guide rails (14) of Y-axis planer-type, and X-axis beam guideway (12) moves horizontally under the effect of Y-axis servomotor (13) on the two guide rails (14) of Y-axis planer-type;
Described mechanical manipulator digitial controller (2) connects X-axis servomotor (11), Y-axis servomotor (13) and Z axis servomotor (15), for controlling magnetic probe (5) in the inner movement of body (4).
3. a kind of magnetic probe search type cell sorting device according to claim 1, it is characterized in that described testing cassete (7) is provided with three containers, be respectively sampling receptacle (71), clean container (72) and target cell (10) are collected container (73), sampling receptacle is equipped with sample liquid in (71), clean container is equipped with scavenging solution in (72), magnetic probe (5) stretches in sampling receptacle (71) and regains after collection adsorption target cell (10), move to again and extend into cleaning DC thing (101) in clean container (72), finally in target cell (10) is collected container (73), target cell (10) is discharged.
4. a kind of magnetic probe search type cell sorting device according to claim 3, is characterized in that described release magnet (8) is placed in body (4) inside, be positioned at target cell (10) collect container (73) under.
5. according to a kind of magnetic probe search type cell sorting device described in any one in the claims, it is characterized in that described magnetic probe (5) end also comprises an isolation outer cover (52) being set with removably, the cover mouth edge of described isolation outer cover (52) has the outward extending limit (53) of prolonging of a circle.
6. a kind of magnetic probe search type cell sorting device according to claim 5, characterized by further comprising an isolation outer cover (52) handling frame, described isolation outer cover (52) handling are placed on testing cassete (7) side or are installed on the edge of body (4), isolation outer cover (52) handling mount and comprise encapsulation parts and dismounting parts, encapsulation parts and dismounting parts all have a probe draw-in groove (91) that holds magnetic probe (5), the upper end place of probe draw-in groove (91) has a spacing and is greater than isolation outer cover (52) internal diameter, be less than the baffle plate (92) that prolongs limit (53) internal diameter of isolation outer cover (52),
Isolation outer cover (52) can be positioned in the draw-in groove of encapsulation parts, and shelves are blocked on baffle plate (92) in the limit (53) of prolonging of isolation outer cover (52);
Stretch in the draw-in groove of dismantling parts with the magnetic probe of isolating outer cover (52) after, up move, the limit (53) of prolonging of isolation outer cover (52) is stopped by baffle plate (92), completes the dismounting of isolation outer cover (52).
7. a kind of magnetic probe search type cell sorting device according to claim 6, characterized by further comprising a discarded isolation outer cover disposable box (54), and described discarded isolation outer cover disposable box (54) is placed under isolation outer cover handling framves (9).
8. a kind of magnetic probe search type cell sorting device according to claim 1, is characterized in that described magnetic probe (5) is for changeable type probe, and magnetic probe (5) is permanent magnetism probe or electromagnetic probe.
9. a kind of magnetic probe search type cell sorting device according to claim 3, it is characterized in that described (7) three containers of testing cassete: sampling receptacle (71), clean container (72) and target cell (10) are collected container (73), have at least two groups to form side by side, meanwhile, the quantity of magnetic probe (5) and the quantity of groups of containers are consistent.
10. according to a kind of magnetic probe search type cell sorting device described in claim 1 or 3, it is characterized in that described sample liquid is blood, marrow, body fluid or the liquid that contains viable cell.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106092174A (en) * | 2016-08-24 | 2016-11-09 | 北京主导时代科技有限公司 | A kind of carrier localization method based on sensor technology and positioner |
| CN106841037A (en) * | 2017-03-14 | 2017-06-13 | 骏实生物科技(上海)有限公司 | A kind of magnetisable material captures analytical equipment and its application |
| CN109971615A (en) * | 2019-04-24 | 2019-07-05 | 重庆工商大学 | A kind of device of magnetic guidance assembling cell microcarrier |
| CN110919634A (en) * | 2019-12-12 | 2020-03-27 | 重庆工商大学 | Device for enhancing magnetic induction intensity by arranging permanent magnets in tree root-shaped multi-layer manner |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106092174A (en) * | 2016-08-24 | 2016-11-09 | 北京主导时代科技有限公司 | A kind of carrier localization method based on sensor technology and positioner |
| CN106841037A (en) * | 2017-03-14 | 2017-06-13 | 骏实生物科技(上海)有限公司 | A kind of magnetisable material captures analytical equipment and its application |
| CN109971615A (en) * | 2019-04-24 | 2019-07-05 | 重庆工商大学 | A kind of device of magnetic guidance assembling cell microcarrier |
| CN109971615B (en) * | 2019-04-24 | 2022-06-03 | 重庆工商大学 | Device for assembling cell microcarrier through magnetic guidance |
| CN110919634A (en) * | 2019-12-12 | 2020-03-27 | 重庆工商大学 | Device for enhancing magnetic induction intensity by arranging permanent magnets in tree root-shaped multi-layer manner |
| CN110919634B (en) * | 2019-12-12 | 2023-02-07 | 重庆工商大学 | Device for enhancing magnetic induction intensity by arranging permanent magnets in tree root-shaped multi-layer manner |
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