CN103641810B - Polyketone compound, preparation method and application thereof - Google Patents
Polyketone compound, preparation method and application thereof Download PDFInfo
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- CN103641810B CN103641810B CN201310729537.7A CN201310729537A CN103641810B CN 103641810 B CN103641810 B CN 103641810B CN 201310729537 A CN201310729537 A CN 201310729537A CN 103641810 B CN103641810 B CN 103641810B
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- C—CHEMISTRY; METALLURGY
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- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
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Abstract
The invention discloses a polyketone compound. The chemical structure formula of the polyketone compound is shown in the specification. The invention also discloses a preparation method of the polyketone compound. The preparation method comprises the steps of performing fermentation culture on fusarium ZS07 (Fusarium proliferatum ZS07) with a preservation number of CCTCC NO:M2013257; filtering the obtained fermentation solution, extracting the filtered solution by using ethyl acetate, concentrating in vacuum and drying to obtain a crude extract; performing silica gel column chromatography fragmentation on the crude extract, and performing gradient elution by using dichloromethane/methyl alcohol; and concentrating an elution part F2 and then recrystallizing to obtain a polyketone compound. The polyketone compound can be used as an herbicide and in particular can be used for suppressing growth of amaranthus retroflexus radicles.
Description
Technical field
The present invention relates to biological technical field, particularly relate to preparation and the weeding application of katydid's commensal gut bacteria strain and new active metabolite thereof.
Background technology
Amaranthus retroflexus (Amaranthus retroflexus L) is the typical pernicious invasive weed of China, and serious harm dry field farming thing, affects crop yield.Chemical herbicide was once the major measure of control Amaranthus retroflexus harm, but the life-time service of chemical herbicide causes environmental pollution, many negative impacts such as disruption of ecological balance.Crave for the background of safe and effective, free of contamination new herbicides the mankind under, research that is efficient, environmental protection microbial herbicide is more and more subject to the attention of expert and scholar in world wide.
Insect fungal component is that a class studies less particular surroundings microorganism.Relative to Lu Sheng soil derived microbial, it has the diversity originated widely and enrich, simultaneously fungal component with the long-term common evolutionary process of host in define unique physiological property and pathways metabolism, be the ideal source of new active metabolite.
A kind of existing compound, its structural formula is as shown in Equation 1; At document Ma S M, Zhan J, Xie X, et al.Redirectingthe cyclization steps of fungal polyketide synthase [J] .Journal of the American Chemical Society, 2008, corresponding informing is had in 130 (1): 38-39, but there are no weeding activity and other any purposes reports.
Bacterial strain F.Proliferatum (FJ 648201) is a kind of crop pathogenic bacterium in agriculture production, can cause maize kernel rot (Lu Weihong, Huang Siliang, department victory beautiful, etc.; Chinese Plants pathology meeting Annual Conference collection of thesis in 2010), F.Proliferatum can produce fumonisin, various toxicity (the Molecular biodiversity ofmycotoxigenic fungi that threaten food safety.Int.J.Food microbiol.2013 with species specificity can be caused to livestock and poultry and laboratory animal, 167,57-66).
Publication number is that the patent of 101928672A informs: fusarium prolifertum can induce dragon tree to produce dragon's blood.
Publication number is that the patent of 103271022A informs: swollen leg honeybee is carried fusarium prolifertum and can be used for preventing and treating poplar ten spot buprestid beetle larva;
The article that the Ji Zhiqin of Xibei Univ. of Agricultural & Forest Science & Technology delivers informs: the meta-bolites of celastrus angulatus endogenetic fungus fusarium prolifertum has obvious fungicidal activity to various agricultural pathogenic fungi.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of polyketides and uses thereof.
In order to solve the problems of the technologies described above, the invention provides a kind of polyketides, its chemical structural formula is:
The present invention also provides a kind of fusarium prolifertum ZS07 (Fusarium proliferatum ZS07) simultaneously, and its preserving number is: CCTCC NO:M2013257.
The present invention also provides the preparation method of above-mentioned polyketides simultaneously, comprises the steps:
1) be, that the fusarium prolifertum ZS07 (Fusarium proliferatum ZS07) of CCTCC NO:M2013257 is inoculated on ME substratum by preserving number, on shaking table, cultivate 2 ~ 3 days 160 ~ 200rpm (being preferably 180rpm), 27.5 ~ 28.5 DEG C (being preferably 28 DEG C), obtain seed liquor;
2), seed liquor to be inoculated in ME substratum (generally speaking, the seed liquor of 10ml is inoculated in the ME substratum of 350 ~ 450ml), 160 ~ 200rpm (being preferably 180rpm), 27.5 ~ 28.5 DEG C (being preferably 28 DEG C) fermentation, 6.5 ~ 7.5 days (being preferably 7 days); Obtain fermented liquid;
3), by step 2) gained filtering fermentation liquor, filtrate ethyl acetate (for isopyknic ethyl acetate) extracts, and vacuum concentration drying (in the vacuum tightness of 0.1 negative pressure, 45 DEG C of dry 30-50 minute), obtains crude extract;
4), by step 3) gained crude extract carries out silica gel column chromatography segmentation, adopt methylene chloride/methanol to carry out gradient elution, the volume ratio of described methylene dichloride and methyl alcohol is followed successively by 100:0,100:1,100:2,100:4,100:8,100:16,100:32; Thus obtain 7 elution fraction F1 ~ F7 respectively;
5), by concentrated for elution fraction F2 (distillation and concentration) recrystallization afterwards, polyketides is obtained.
Improvement as the preparation method of polyketides of the present invention: ME substratum is: malt extract 20g, sucrose 20g, peptone 1g, distilled water is settled to 1L.
The present invention also provides the purposes of above-mentioned polyketides simultaneously: as weedicide.
Improvement as the purposes of polyketides of the present invention: the growth suppressing Amaranthus retroflexus young root.
The present invention adopts culture dish-filter paper enzyme to measure new compound to the restraining effect of Amaranthus retroflexus seed young root.
The present invention relates to preparation and the weeding application of the new active metabolite of a kind of katydid's commensal gut bacterium.Be separated from healthy katydid's enteron aisle and obtain symbiotic effects bacterial strain Fusarium proliferatum ZS07, this bacterial strain is preserved in China typical culture collection center (CCTCC), and its deposit number is CCTCC NO:M2013257.Described compound is novel compound, has no report before.Described purposes is embodied in this novel compound and invasive weed Amaranthus retroflexus young root can be suppressed to grow.Strain fermentation culture condition of the present invention is simple, and new compound extraction process is clear and definite, suppresses Amaranthus retroflexus young root growth result obvious.Can be used as agricultural herbicide.
Polyketides of the present invention is as usage during weedicide and consumption: be diluted to 0.05 ~ 0.1g/L and spray; The consumption (significant quantity) of per hectare is about 50 ~ 100g.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is cultural characteristic and the spore shape characteristic pattern of katydid's commensal gut bacterium (F.Proliferatum ZS07) bacterial strain;
The cultural characteristic figure of Zuo Tuwei katydid's commensal gut bacterium (F.Proliferatum ZS07) bacterial strain;
Right figure is katydid's commensal gut bacterium (F.Proliferatum ZS07) bacterial strain spore shape characteristic pattern.
Fig. 2 is the weeding activity comparison diagram of polyketides and 2,4-D.
Embodiment
The experimental technique used in following embodiment is ordinary method if no special instructions.
Described reagent all can obtain from commercial channels.
Embodiment 1, bacterial strain screening:
In November, 2012 catches healthy katydid's sample from suburb, Jinhua, and sample is taken back laboratory as early as possible and processed.
Substratum is MEA substratum: malt extract 20g, sucrose 20g, agar 20g, peptone 1g, and distilled water is settled to 1L, pH 7.0.1.1 normal atmosphere, sterilizing 20min (for conventional sterilant) at 121 DEG C.
Method: aseptically use 75% (volume %) alcohol surface sterilization 2min after the hungry 24h of healthy Tettigonionidae, dissect after rinsed with sterile water 3 times, get enteron aisle to add a small amount of sterilized water and grind in mortar and obtain suspension, with sterilized water respectively gradient dilution 10
1, 10
2, 10
3doubly, get each gradient dilution liquid 0.2mL and coat on MEA culture medium flat plate, in 28 DEG C of thermostat containers, cultivate 2-3 days.After bacterium colony grows, a small amount of mycelia of picking is transferred in new MEA culture medium flat plate purifying and obtains single bacterium colony, by this bacterium colony---and fungal component ZS07 is inoculated into MEA slant tube and saves backup.
Embodiment 2, identification of strains
ITS rDNA analyzes:
According to BioTeke novel quick-speed plant genome DNA extracting reagent kit specification sheets, extract the fungal component ZS07 genomic dna of above-described embodiment 1 gained, adopt ITS universal primer ITS1 (5 '-TCCGTAGGTGAACCTGCGG-3 ' forward) and ITS4 (5 '-TCCTCCGCTTATTGATATGC-3 ', the rDNA ITS district's gene order that oppositely) increases purifying.
Sequencing result carries out similarity-rough set by blast program; Analytical results shows, symbiotic effects ZS07 and bacterial strain F.Proliferatum (FJ 648201) has higher similarity, and similarity is 99.5%.In conjunction with this bacterium morphological feature, identify that this bacterium is F.Proliferatum.
This bacterium is carried out preservation, depositary institution: China typical culture collection center; Preservation name is called: sickle-like bacteria ZS07 (Fusarium proliferatum ZS07), preservation address: Wuhan, China Wuhan University; Preservation date: 2013.06.13; Preserving number is: CCTCC NO:M2013257.
The preparation of embodiment 3, polyketide
By the mycelium block (0.5 × 0.5cm of fresh sickle-like bacteria ZS07 (Fusarium proliferatum ZS07)
2, about 0.5g) and be inoculated into every bottle containing in the 250mL Erlenmeyer flask of 150mL ME substratum, inoculate 5 ~ 6 bottles, cultivate 2 ~ 3 days under 180rpm, 28 DEG C of conditions as seed liquor.
Remarks illustrate: sickle-like bacteria ZS07 after Fructus Hordei Germinatus solid medium (MEA substratum) activation of routine, fresh sickle-like bacteria ZS07.
10mL seed liquor is inoculated in the 1000mL Erlenmeyer flask of the ME substratum that 400mL is housed, 180rpm, 28 DEG C of condition bottom fermentations 7 days.
The two-layer filtered through gauze of fermented liquid, filtrate uses equal-volume extraction into ethyl acetate, and extraction times is 3 times, and vacuum concentration drying (in the vacuum tightness of 0.1 negative pressure, 45 DEG C of dry 30-50 minute), obtains crude extract 40.5g.
Above-mentioned ME substratum is: malt extract 20g, sucrose 20g, peptone 1g, and distilled water is settled to 1L, natural PH, then 1.1 normal atmosphere, sterilizing 20min (for conventional sterilant) at 121 DEG C.
(described silicagel column selects 200 ~ 300 object silica gel to carry out silica gel column chromatography segmentation to this 40.5g crude extract, weight is 150g), adopt methylene chloride/methanol gradient elution, the volume ratio of methylene chloride/methanol is followed successively by 100:0,100:1,100:2,100:4,100:8,100:16,100:32, the consumption of often kind of elutriant is respectively about 1500ml, about 1200ml, about 1000ml, about 1000ml, about 800ml, about 800ml, about 800ml; Thus obtain 7 elution fraction F1-F7 respectively.In methyl alcohol, carry out recrystallization after the 2nd of gained kind of elution fraction F2 is carried out distillation and concentration (in 45 DEG C of dry 40-55 minute), the light yellow crystal of gained is polyketides.
The Structural Identification of embodiment 4, polyketide
The polyketides of embodiment 3 gained is light yellow crystal, and ESI-MS show that compound molecular weight is m/z 357 [M+H]
+, molecular formula is C
19h
16o
7.
1h-NMR,
13c-NMR determines this compound structure in conjunction with 2D-NMR spectrum analysis.
Its chemical structural formula is:
Spectral data is:
1h NMR (Acetone-d
6) δ: 2.43 (3H, s, H-18), 4.25 (2H, s, H-10), 6.30 (1H, d, J=2.3Hz, H-8), 6.32 (1H, d, J=2.3Hz, H-16), 6.51 (1H, d, J=2.3Hz, H-14), 6.59 (1H, d, J=2.3Hz, H-4), 6.62 (1H, s, H-6), 11.14 (1H, s, 3-OH), 3.93 (3H, s, OCH
3).
13c NMR (Acetone-d
6) δ: 21.5 (C-18), 47.8 (C-10), 55.4 (5-OCH
3), 100.1 (C-2), 100.4 (C-14), 101.3 (C-4), 102.9 (C-6), 107.0 (C-8), 111.0 (C-16), 118.9 (C-12), 139.5 (C-7), 141.2 (C-17), 152.4 (C-9), 156.4 (C-13), 160.8 (C-15), 163.4 (C-3), 166.9 (C-5), 167.1 (C-1), 198.9 (C-11).
The polyketides of embodiment 5, embodiment 3 gained is to the restraining effect of Amaranthus retroflexus young root
Get proper amount of acetone and this compound dissolution is mixed with 10 μ g/mL, 1 μ g/mL diluent, add 5ml diluent in the culture dish being covered with filter paper, make filter paper and liquid fully saturated, after acetone volatilization, add 5ml sterilized water.Blank acetone does same process.Same concentration (that is, 10 μ g/mL, 1 μ g/mL) 2,4-D is as positive control.Choosing 150, the Amaranthus retroflexus seed (10, every ware) just showed money or valuables one carries unintentionally is placed on the filter paper of above-mentioned process, 27 DEG C, 80% relative humidity, timing illumination (12h illumination, 12h dark) condition artificial climate incubator cultivate 4d.Measure young root length, represent result with average root growth inhibition ratio, each process repeats 3 wares.
Average production inhibiting rate (%)=[contrast average root length-process average root is long]/contrast average root long × 100%.
As shown in Figure 2, result shows that this polyketides has restraining effect to Amaranthus retroflexus growth to experimental result.When concentration is 1 μ g/mL, new compound is 42.1% to the inhibiting rate that Amaranthus retroflexus young root grows, a little more than the positive control 2,4-D (40.1%) of comparable sodium.When concentration increases to 10 μ g/mL, new compound and positive drug all show stronger Amaranthus retroflexus young root growth inhibitory activity, and wherein this compound is 67.1% to Amaranthus retroflexus young root inhibiting rate, and under the same terms, positive drug 2,4-D inhibiting rate is 72.7%.
Above result prompting, this polyketides has inhibit activities to the growth of Amaranthus retroflexus young root, can as weedicide.
Comparative example 1, the compound described in the formula 1 of informing in background technology to be detected according to method described in embodiment 5, no matter be when concentration is 1 μ g/mL, or when concentration is 10 μ g/mL, the inhibiting rate of the compound described in formula 1 to Amaranthus retroflexus young root is 0%.That is, the compound described in formula 1 does not have inhibit activities to the growth of Amaranthus retroflexus young root, can not as weedicide.
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
<110> Zhejiang Normal University
<120> polyketides and its production and use
<160> 2
<210> 1
<211> 19
<212> DNA
<213> artificial sequence
<220>
<223> primer I TS1
<400> 1
tccgtaggtg aacctgcgg 19
<210> 2
<211> 20
<212> DNA
<213> artificial sequence
<220>
<223> primer I TS4
<400> 2
tcctccgctt attgatatgc 20
Claims (6)
1. polyketides, is characterized in that chemical structural formula is:
2. fusarium prolifertum ZS07 (Fusarium proliferatum ZS07), its preserving number is: CCTCC NO:M2013257.
3. the preparation method of polyketides as claimed in claim 1, is characterized in that comprising the steps:
1) be, that the fusarium prolifertum ZS07 (Fusarium proliferatum ZS07) of CCTCC NO:M2013257 is inoculated on ME substratum by preserving number, on shaking table, 160 ~ 200rpm, 27.5 ~ 28.5 DEG C cultivate 2 ~ 3 days, obtain seed liquor;
2), by seed liquor be inoculated in ME substratum, 160 ~ 200rpm, 27.5 ~ 28.5 DEG C fermentation 6.5 ~ 7.5 days; Obtain fermented liquid;
3), by step 2) gained filtering fermentation liquor, filtrate is extracted with ethyl acetate, and vacuum concentration is dry, obtains crude extract;
4), by step 3) gained crude extract carries out silica gel column chromatography segmentation, adopt methylene chloride/methanol to carry out gradient elution, the volume ratio of described methylene dichloride and methyl alcohol is followed successively by 100:0,100:1,100:2,100:4,100:8,100:16,100:32; Thus obtain 7 elution fraction F1 ~ F7 respectively;
5), by concentrated for elution fraction F2 rear recrystallization, polyketides is obtained.
4. the preparation method of polyketides according to claim 3, is characterized in that: described ME substratum is: malt extract 20g, sucrose 20g, peptone 1g, distilled water is settled to 1L.
5. the purposes of polyketides as claimed in claim 1, is characterized in that: as weedicide.
6. the purposes of polyketides according to claim 5, is characterized in that: the growth suppressing Amaranthus retroflexus young root.
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| CN113278531B (en) * | 2021-04-25 | 2023-07-04 | 中国热带农业科学院热带生物技术研究所 | Inducer for inducing Hainan dragon tree to rapidly generate dragon's blood and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785993A (en) * | 2005-12-14 | 2006-06-14 | 南开大学 | Preparation method of non natural isocumarin and its 3,4-dihydro derivative and use thereof |
| CN101880633A (en) * | 2010-06-25 | 2010-11-10 | 河北省农林科学院植物保护研究所 | Fusarium proliferatum, and bacterium agent and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785993A (en) * | 2005-12-14 | 2006-06-14 | 南开大学 | Preparation method of non natural isocumarin and its 3,4-dihydro derivative and use thereof |
| CN101880633A (en) * | 2010-06-25 | 2010-11-10 | 河北省农林科学院植物保护研究所 | Fusarium proliferatum, and bacterium agent and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 3株镰刀菌的鉴定、对野生燕麦的致病力及其对5种作物的影响;朱海霞等;《中国生物防治》;20101115;第26卷(第S1期);第84-89页 * |
| Classification, Prediction, and Verification of the Regioselectivity of Fungal Polyketide Synthase Product Template Domains;Yanran Li et al.;《Journal of Biological Chemistry》;20100517;第285卷(第30期);第22764-22773页 * |
| Redirecting the Cyclizaiton Steps of Fungal Polyketide Synthase;Suzanne M. Ma et al.;《J. Am. Chem. Soc》;20071212;第130卷(第1期);第38-39页 * |
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