CN103626779B - A kind of extracting method of chlorophyll from luffa leaf - Google Patents
A kind of extracting method of chlorophyll from luffa leaf Download PDFInfo
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- CN103626779B CN103626779B CN201310644685.9A CN201310644685A CN103626779B CN 103626779 B CN103626779 B CN 103626779B CN 201310644685 A CN201310644685 A CN 201310644685A CN 103626779 B CN103626779 B CN 103626779B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- Organic Chemistry (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
The invention discloses a kind of extracting method of chlorophyll from luffa leaf, the method is using aqueous ethanol as extraction agent, adopt ultrasonic-assisted extraction technology, natural chlorophyll is extracted from Leaf of Luffa, concrete technology flow process is: carried out grinding process by Leaf of Luffa, the aqueous ethanol adding 60% ~ 80% by the solid-liquid ratio of 1:20 ~ 40 adopts Ultrasonic Wave-Assisted Extraction, carries out vacuum distilling and concentrates, obtain chlorophyll medicinal extract after being filtered by vat liquor.The present invention changes the extracting technology of the hazardous solvents such as original acetone, decreases industrial pollution, instead of original toxic reagent, not only effectively raise extracting efficiency with ethanol, and greatly reduces the injury of objectionable impurities to human body.
Description
Technical field
The present invention relates to chemical extraction field, be specifically related to a kind of extracting method of chlorophyll from luffa leaf.
Background technology
Chlorophyll is that plant carries out photosynthetic primary pigments, is a kind of pigment family containing fat, is positioned at thylakoid membrane.Chlorophyll is magnesium porphyrin compound, comprises chlorophyll a, b, c, d, f and protochlorophyll and bacteriochlorophyll etc.Chlorophyll is not bery stable, and light, acid, alkali, oxygen, oxygenant etc. all can make it decompose.Under acidic conditions, chlorophyll molecule is easy to the magnesium lost in porphyrin ring becomes de-magnesium pheophytin.Chlorophyll is present in most of green plants widely, there is hematopoiesis, beauty treatment and the multiple efficacies such as antiviral, but chlorophyllous extraction is still adopted mostly to the method for acetone and ether, these two kinds of organic reagents itself have very large toxicity, also can with certain injury to human body, a large amount of uses of this toxic reagent in industrialized production, can bring very large pollution to environment.
Ethanol, as a kind of conventional extraction reagent, does not have toxicity, water white transparency, volatile, and easily dissolving each other with most of organic solvent and water, and can dissolve gas chromatography and inorganics, is a kind of conventional extraction reagent.
Wherein sponge gourd is not utilized as the general branches and leaves of a kind of vegetables, but containing a large amount of effective constituent materials in sponge gourd branches and leaves, simultaneously containing abundant chlorophyll, has good health-care effect, therefore cause a large amount of wastes of effective constituent in sponge gourd branches and leaves.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of extracting method of chlorophyll from luffa leaf.
The invention provides a kind of extracting method of chlorophyll from luffa leaf, the method is using aqueous ethanol as extraction agent, adopt ultrasonic-assisted extraction technology, natural chlorophyll is extracted from Leaf of Luffa, concrete technology flow process is: carried out grinding process by Leaf of Luffa, the aqueous ethanol adding 60% ~ 80% by the solid-liquid ratio of 1:20 ~ 40 adopts Ultrasonic Wave-Assisted Extraction, carries out vacuum distilling and concentrates, obtain chlorophyll medicinal extract after being filtered by vat liquor.
The technique of Ultrasonic Wave-Assisted Extraction of the present invention is: ultrasonic frequency is 20kHz, and power is 40w, and ultrasonic time is 15min.
The present invention adds the cellulase of 0.03 ~ 0.05% when Ultrasonic Wave-Assisted Extraction, promote that cell walls is broken, improve chlorophyll leaching yield.
The temperature of vacuum distilling of the present invention controls at 70 ~ 90 DEG C, prevents chlorophyll degrading activity from reducing.
The invention has the advantages that: the extracting method of this chlorophyll from luffa leaf, change the extracting technology of the hazardous solvents such as original acetone, original toxic reagent is instead of with ethanol, not only effectively raise chlorophyllous extracting efficiency, reduce production cost, and greatly reduce the injury of objectionable impurities to human body, decrease industrial pollution, be suitable for industrialized production, abundant extraction sponge gourd Determination of Chlorophyll composition, improve the effective rate of utilization of Leaf of Luffa, reduce the wasting of resources, expand economic benefit.
embodiment:
Embodiment one:
Leaf of Luffa is carried out grinding process, the aqueous ethanol adding 80% by the solid-liquid ratio of 1:20 adopts Ultrasonic Wave-Assisted Extraction, its ultrasonic frequency is 20kHz, power is 40w, ultrasonic time is 15min, adds the cellulase of 0.03% during lixiviate, carries out vacuum distilling and concentrate after being filtered by vat liquor, the temperature of vacuum distilling is 70 DEG C, finally obtains chlorophyll medicinal extract.
Embodiment two:
Leaf of Luffa is carried out grinding process, the aqueous ethanol adding 60% by the solid-liquid ratio of 1:40 adopts Ultrasonic Wave-Assisted Extraction, its ultrasonic frequency is 20kHz, power is 40w, ultrasonic time is 15min, adds the cellulase of 0.05% during lixiviate, carries out vacuum distilling and concentrate after being filtered by vat liquor, the temperature of vacuum distilling is 90 DEG C, finally obtains chlorophyll medicinal extract.
Embodiment three:
Leaf of Luffa is carried out grinding process, the aqueous ethanol adding 70% by the solid-liquid ratio of 1:30 adopts Ultrasonic Wave-Assisted Extraction, its ultrasonic frequency is 20kHz, power is 40w, ultrasonic time is 15min, adds the cellulase of 0.04% during lixiviate, carries out vacuum distilling and concentrate after being filtered by vat liquor, the temperature of vacuum distilling is 80 DEG C, finally obtains chlorophyll medicinal extract.
Claims (1)
1. the extracting method of a chlorophyll from luffa leaf, it is characterized in that: using aqueous ethanol as extraction agent, adopt ultrasonic-assisted extraction technology, natural chlorophyll is extracted from Leaf of Luffa, concrete technology flow process is: carried out grinding process by Leaf of Luffa, the aqueous ethanol adding 60% ~ 80% by the solid-liquid ratio of 1:20 ~ 40 adopts Ultrasonic Wave-Assisted Extraction, carries out vacuum distilling and concentrates, obtain chlorophyll medicinal extract after being filtered by vat liquor; The technique of described Ultrasonic Wave-Assisted Extraction is: ultrasonic frequency is 20kHz, and power is 40w, and ultrasonic time is 15min, adds the cellulase of 0.03 ~ 0.05% when Ultrasonic Wave-Assisted Extraction; The temperature of described vacuum distilling controls at 70 ~ 90 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644685.9A CN103626779B (en) | 2013-12-05 | 2013-12-05 | A kind of extracting method of chlorophyll from luffa leaf |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310644685.9A CN103626779B (en) | 2013-12-05 | 2013-12-05 | A kind of extracting method of chlorophyll from luffa leaf |
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| Publication Number | Publication Date |
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| CN103626779A CN103626779A (en) | 2014-03-12 |
| CN103626779B true CN103626779B (en) | 2016-03-16 |
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| CN201310644685.9A Active CN103626779B (en) | 2013-12-05 | 2013-12-05 | A kind of extracting method of chlorophyll from luffa leaf |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923091B (en) * | 2014-04-22 | 2016-03-30 | 山西大学 | A kind of chlorophyllous method of extraction |
| CN104678066A (en) * | 2015-03-17 | 2015-06-03 | 内蒙古民族大学 | Method for recycling chlorophyll solution |
| CN105037377A (en) * | 2015-07-30 | 2015-11-11 | 潍坊友容实业有限公司 | Extraction preparation method for sodium ferrous chlorophyllin and sodium ferrous chlorophyllin prepared through extraction preparation method |
| CN105037378A (en) * | 2015-07-30 | 2015-11-11 | 潍坊友容实业有限公司 | Method for preparing and extracting iron chlorophyll and prepared iron chlorophyll |
| CN106083865A (en) * | 2016-06-21 | 2016-11-09 | 太仓市东明化工有限公司 | A kind of chlorophyllous method in high efficiency extraction Folium Bambusae |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229612A (en) * | 2011-04-20 | 2011-11-02 | 晨光生物科技集团天津有限公司 | Method for extracting chlorophyll Cu from silkworm excrements |
| CN103146223A (en) * | 2013-03-18 | 2013-06-12 | 徐静 | Ultrasonic extraction method of exocarpium benincasae pigment |
| CN103275090A (en) * | 2013-06-14 | 2013-09-04 | 北京林业大学 | Method for rapidly extracting fat soluble chlorophyll from pine needles by combining enzymatic hydrolysis with flash-type extraction |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6185A (en) * | 1984-06-08 | 1986-01-06 | Ihara Chem Ind Co Ltd | Industrial process for producing chlorophyll |
| JPH06185A (en) * | 1992-06-22 | 1994-01-11 | Matsushita Electric Ind Co Ltd | Device for washing ultrasonic probe connector pin |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229612A (en) * | 2011-04-20 | 2011-11-02 | 晨光生物科技集团天津有限公司 | Method for extracting chlorophyll Cu from silkworm excrements |
| CN103146223A (en) * | 2013-03-18 | 2013-06-12 | 徐静 | Ultrasonic extraction method of exocarpium benincasae pigment |
| CN103275090A (en) * | 2013-06-14 | 2013-09-04 | 北京林业大学 | Method for rapidly extracting fat soluble chlorophyll from pine needles by combining enzymatic hydrolysis with flash-type extraction |
Non-Patent Citations (1)
| Title |
|---|
| "菠菜叶绿素的提取及叶绿素铜钠盐的制备";陈小全 等;《泰山医学院学报》;20101231;第31卷(第4期);第259-261页 * |
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| CN103626779A (en) | 2014-03-12 |
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Effective date of registration: 20160823 Address after: 99 No. 236500 Anhui city of Fuyang Province Jieshou City feigexin Avenue Patentee after: ANHUI FINDSHE BIOLOGICAL PHARMACEUTICAL CO., LTD. Address before: 99 No. 236500 Anhui city of Fuyang Province Jieshou City feigexin Avenue Patentee before: Anhui Funland Biological Pharmaceutical Co., Ltd. |