CN103623785B - Can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method - Google Patents
Can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method Download PDFInfo
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- CN103623785B CN103623785B CN201310669563.5A CN201310669563A CN103623785B CN 103623785 B CN103623785 B CN 103623785B CN 201310669563 A CN201310669563 A CN 201310669563A CN 103623785 B CN103623785 B CN 103623785B
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Abstract
The invention discloses can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method.By NH
4hCO
3solution, NH
4h
2pO
4solution, Ca (NO
3)
2solution, NH
4oH solution, hydrochloric acid solution, NH
4the Cl aqueous solution and Ca-EDTA potassium solution are prepared into mixed liquor F.Poly beta-hydroxybutyrate chloroformic solution and polyethylene glycol chloroformic solution are prepared into mixed liquor M.Mixed liquor F is added drop-wise in mixed liquor M, obtains mixed liquor O; Spurted in the container that liquid nitrogen is housed by distilled water and make ice hockey particle, it is real that screening is placed on mould inner pressure; Mixed liquor O is poured in mould freeze settled, through the demoulding and freezing after cultivate in petroleum hydrocarbon degradation bacteria culture fluid 24h take out air-dry, can obtain can slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon suspension oil absorption plate.Manufacture craft of the present invention is simple, and obtained suspension oil absorption plate is high to petroleum hydrocarbon class pollutant treatment effeciency, and can not cause secondary pollution and destruction to water ecological setting.
Description
Technical field
The invention belongs to the processing technology field by petroleum hydrocarbon contaminated water body, particularly a kind of can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method.
Background technology
In recent years, along with the demand that countries in the world day by day increase oil and goods thereof, exploit, load and unload, transport and utilize oil spill accident in oil process to happen occasionally at sea, a large amount of crude oil is not only caused to run off, also severe contamination is caused to environment, threaten the ecological environment of water body and land-based area with being on the rise, have long-term harm to water body environment, natural resources and culture resources etc., therefore, water body oil spill become people must faced by great environmental problem.
The method disposing water body oil spill at present both at home and abroad mainly contains Physical, chemical method and bioanalysis.Chemical method is the technology path of comparative maturity in point-source pollution is administered, and has obtained propagation and employment widely.But adopt chemical method in water body oil spill is administered, need to add chemical agent in water body, the chemical agent wherein added may produce secondary pollution on the one hand, can produce short-term or long-term impact on the other hand on water ecological setting.The features such as bioremediation technology has efficiently, economy, safety, non-secondary pollution, become the on-the-spot important selection approach removing water body oil spill, particularly to the oil sheet that mechanical device cannot be removed, when simultaneously restriction uses chemical agent again, use and biological prostheticly show its larger superiority.Physisorphtion is that a kind for the treatment of effeciency is high, cost is low, without sediment pollution, simple to operate and can not cause the method for secondary pollution and destruction to water ecological setting, can solve the problem existing for chemical method preferably.But, the cycle of bioremediation technology is long, physisorphtion faces the saturated rear problem needing to remarket adsorbent of absorption, if a kind of biodegradable adsorbent can be prepared, fix petroleum hydrocarbon degradation bacterium on the sorbent to improve the clearance of petroleum hydrocarbon simultaneously, after absorption is saturated, pass through the effect of microbe inoculation by adsorbent petroleum hydrocarbon degradation, and final biodegradable adsorbent itself also thoroughly can be degraded, then can address this problem.Physisorphtion and fixation of microbe being carried out enhancing degradation combine, larger advantage will be had for disposing water body oil spill.
In the process of microbial degradation petroleum hydrocarbon and adsorbent itself, need the participation of phosphorus nutrition element, the nutrients such as phosphorus contained in oil spill water body under normal circumstances cannot meet the needs of microorganism, but the adsorbent with slowly-releasing phosphorus function can meet this requirement.The technology combined to physisorphtion and fixation of microbe be carried out enhancing degradation obtains further propagation and employment in the process of water body oil spill, need to develop the material simultaneously with slowly-releasing P elements and physical absorption-biodegradation of petroleum hydrocarbon function, this has great importance for raising water body oil spill treatment effeciency, reduction environmental pollution, but the technology also lacking this aspect is at present reported with research.
Summary of the invention
The object of this invention is to provide a kind of can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method.Its concrete steps are as follows:
(1) be the NH of 0.35mol/L by 100mL concentration
4hCO
3solution and 100mL concentration are the NH of 0.62mol/L
4h
2pO
4mixing, obtain mixed liquor A l, is then the NH of 0.01mol/L by concentration
4oH solution and concentration are that the hydrochloric acid solution of 0.01mol/L regulates the pH value of mixed liquor A l to be 9.0, obtain mixed liquor A;
(2) 200mL mixed liquor A is slowly added drop-wise under agitation the Ca (NO that 300mL concentration is 0.025mol/L
3)
2in solution, obtain mixed liquid B;
(3) in mixed liquid B, dense HNO is dropwise added
3to clarification, obtain mixed liquor C;
(4) regulate the pH value of mixed liquor C to be 3.5 with the salpeter solution of concentration to be the NaOH solution of 0.01mol/L and concentration be 0.01mol/L, obtain mixed liquor D;
(5) in mixed liquor D, add the aqueous solution of urea that 100mL concentration is 1.2mol/L, after mixing, obtain mixed liquor E;
(6) in mixed liquor E, add the calcio-disodium edetate solution that 50mL concentration is 0.15mol/L, after mixing, obtain mixed liquor F;
(7) by 240mL mass fraction be 7.5% poly beta-hydroxybutyrate chloroformic solution and 40mL mass fraction be 5.5% polyethylene glycol chloroformic solution fully mix, obtain mixed liquor M;
(8) 60mL mixed liquor F is added drop-wise in mixed liquor M under 1000r/min stirring condition, then under 1000r/min condition, stirs 8min, obtain mixed liquor O;
(9) distilled water being spurted in the container that liquid nitrogen is housed snap frozen and make ice hockey particle, is that the ice hockey particle of 50 ~ 100 μm is stand-by with choosing grain size scope after sieved through sieve;
(10) ice hockey particle garbled in step (9) is placed in also compacting in mould cavity;
(11) mixed liquor O is poured in the mould that step (10) obtains, and together puts into liquid nitrogen freeze settled 6 hours, after taking out the demoulding, obtain solid mixt; Obtaining after this solid mixt vacuum freeze drying is removed chloroform and ice hockey particle can the suspension oil absorption plate of slow releasing P elements;
(12) the suspension oil absorption plate that step (11) obtains is cultivated 24h in petroleum hydrocarbon degradation bacteria culture fluid, be absorbed and fixed at by petroleum hydrocarbon degradation bacterium in this suspension oil absorption plate, wherein the bacteria containing amount of petroleum hydrocarbon degradation bacteria culture fluid is 5 × 10
10cFU/ml, consisting of of nutrient solution: NH
4nO
3: 2.0gL
-1, NaCl:10.5gL
-1, KH
2pO
4: 2.5gL
-1, FeCl
3: 0.10gL
-1, MgSO
4: 3.5gL
-1, CaCl
22H
2o:0.15gL
-1;
(13) the suspension oil absorption plate that step (12) obtained is taken out air-dry, and can obtain can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon.
The invention has the beneficial effects as follows, manufacture craft is simple, obtained can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon high to petroleum hydrocarbon contaminant treatment effeciency, and secondary pollution and destruction can not be caused to water ecological setting.
Detailed description of the invention
The invention provides a kind of can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method, with an embodiment, the present invention is described below.
Embodiment 1.
Be the NH of 0.35mol/L by 100mL concentration
4hCO
3solution and 100mL concentration are the NH of 0.62mol/L
4h
2pO
4mixing, obtain mixed liquor A l, is then the NH of 0.01mol/L by concentration
4oH solution and concentration are that the hydrochloric acid solution of 0.01mol/L regulates the pH value of mixed liquor A l to be 9.0, obtain mixed liquor A; 200mL mixed liquor A is slowly added drop-wise under agitation the Ca (NO that 300mL concentration is 0.025mol/L
3)
2in solution, obtain mixed liquid B; Dense HNO is dropwise added in mixed liquid B
3to clarification, obtain mixed liquor C; Regulate the pH value of mixed liquor C to be 3.5 with the salpeter solution of concentration to be the NaOH solution of 0.01mol/L and concentration be 0.01mol/L, obtain mixed liquor D; In mixed liquor D, add the aqueous solution of urea that 100mL concentration is 1.2mol/L, after mixing, obtain mixed liquor E; In mixed liquor E, add the calcio-disodium edetate solution that 50mL concentration is 0.15mol/L, after mixing, obtain mixed liquor F;
By 240mL mass fraction be 7.5% poly beta-hydroxybutyrate chloroformic solution and 40mL mass fraction be 5.5% polyethylene glycol chloroformic solution fully mix, obtain mixed liquor M;
60mL mixed liquor F is added drop-wise in mixed liquor M under 1000r/min stirring condition, then under 1000r/min condition, stirs 8min, obtain mixed liquor O;
Distilled water being spurted in the container that liquid nitrogen is housed snap frozen and make ice hockey particle, is that the ice hockey particle of 50 ~ 100 μm to be placed in mould cavity and compacting with choosing grain size scope after sieved through sieve; Mixed liquor O is poured in mould, and together puts into liquid nitrogen freeze settled 6 hours, after taking out the demoulding, obtain solid mixt; Obtaining after this solid mixt vacuum freeze drying is removed chloroform and ice hockey particle can the suspension oil absorption plate of slow releasing P elements; Suspension oil absorption plate being cultivated in petroleum hydrocarbon degradation bacteria culture fluid 24h takes out air-dry, and can obtain can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon.
Here be use the inventive method to obtain the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon can carry out absorption and biodegradation test to the petroleum hydrocarbon in petroleum hydrocarbon contaminated water body, further illustrate the present invention.
That uses the inventive method to obtain the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon can carry out absorption and biodegradation test to the petroleum hydrocarbon in petroleum hydrocarbon contaminated water body, result shows that this suspension oil absorption plate effectively can be adsorbed and biodegradation the petroleum hydrocarbon in water body: when the initial concentration of polluted-water Petroleum Hydrocarbon is 86.4mg/L, 12g oil absorption plate is added in the water body of 500mL, after 10 hours, the concentration of water body Petroleum Hydrocarbon is reduced to 0.01mg/L, within 10 days, the Petroleum Hydrocarbon of suspension oil absorption plate afterwards concentration does not detect, the quality of 150 days suspension oil absorption plate afterwards reduces by 42%.
Claims (1)
1. can the preparation method of suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon, it is characterized in that, the concrete steps of the method are as follows:
(1) be the NH of 0.35mol/L by 100mL concentration
4hCO
3solution and 100mL concentration are the NH of 0.62mol/L
4h
2pO
4mixing, obtain mixed liquor A 1, is then the NH of 0.01mol/L by concentration
4oH solution and concentration are that the hydrochloric acid solution of 0.01mol/L regulates the pH value of mixed liquor A 1 to be 9.0, obtain mixed liquor A;
(2) 200mL mixed liquor A is slowly added drop-wise under agitation the Ca (NO that 300mL concentration is 0.025mol/L
3)
2in solution, obtain mixed liquid B;
(3) in mixed liquid B, dense HNO is dropwise added
3to clarification, obtain mixed liquor C;
(4) regulate the pH value of mixed liquor C to be 3.5 with the salpeter solution of concentration to be the NaOH solution of 0.01mol/L and concentration be 0.01mol/L, obtain mixed liquor D;
(5) in mixed liquor D, add the aqueous solution of urea that 100mL concentration is 1.2mol/L, after mixing, obtain mixed liquor E;
(6) in mixed liquor E, add the calcio-disodium edetate solution that 50mL concentration is 0.15mol/L, after mixing, obtain mixed liquor F;
(7) by 240mL mass fraction be 7.5% poly beta-hydroxybutyrate chloroformic solution and 40mL mass fraction be 5.5% polyethylene glycol chloroformic solution fully mix, obtain mixed liquor M;
(8) 60mL mixed liquor F is added drop-wise in mixed liquor M under 1000r/min stirring condition, then under 1000r/min condition, stirs 8min, obtain mixed liquor O;
(9) distilled water being spurted in the container that liquid nitrogen is housed snap frozen and make ice hockey particle, is that the ice hockey particle of 50 ~ 100 μm is stand-by with choosing grain size scope after sieved through sieve;
(10) ice hockey particle garbled in step (9) is placed in also compacting in mould cavity;
(11) mixed liquor O is poured in the mould that step (10) obtains, and together puts into liquid nitrogen freeze settled 6 hours, after taking out the demoulding, obtain solid mixt; Obtaining after this solid mixt vacuum freeze drying is removed chloroform and ice hockey particle can the suspension oil absorption plate of slow releasing P elements;
(12) the suspension oil absorption plate that step (11) obtains is cultivated 24h in petroleum hydrocarbon degradation bacteria culture fluid, be absorbed and fixed at by petroleum hydrocarbon degradation bacterium in this suspension oil absorption plate, wherein the bacteria containing amount of petroleum hydrocarbon degradation bacteria culture fluid is 5 × 10
10cFU/mL, consisting of of nutrient solution: NH
4nO
3: 2.0gL
-1, NaCl:10.5gL
-1, KH
2pO
4: 2.5gL
-1, FeCl
3: 0.10gL
-1, MgSO
4: 3.5gL
-1, CaCl
22H
2o:0.15gL
-1;
(13) the suspension oil absorption plate that step (12) obtained is taken out air-dry, and can obtain can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310669563.5A CN103623785B (en) | 2013-12-12 | 2013-12-12 | Can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method |
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|---|---|---|---|
| CN201310669563.5A CN103623785B (en) | 2013-12-12 | 2013-12-12 | Can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method |
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|---|---|
| CN103623785A CN103623785A (en) | 2014-03-12 |
| CN103623785B true CN103623785B (en) | 2015-09-09 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004058647A1 (en) * | 2002-12-31 | 2004-07-15 | Ku-Su Kim | Wastewater disposal agent and process for preparing thereof |
| CN102500340A (en) * | 2011-09-30 | 2012-06-20 | 北京师范大学 | Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle |
| CN102963959A (en) * | 2012-12-03 | 2013-03-13 | 北京师范大学 | Preparation method of microsphere particles capable of slowly releasing nitrogen and adsorbing-biologically degrading petroleum hydrocarbon |
| CN102976499A (en) * | 2012-12-03 | 2013-03-20 | 北京师范大学 | Method for preparing composite board capable of simultaneously slowly releasing nitrogen and adsorbing/biologically degrading petroleum hydrocarbons |
| WO2013102729A1 (en) * | 2012-01-03 | 2013-07-11 | Compagnie Financiere Et De Participations Roullier | Phosphate-based granular material, process for manufacturing same and use thereof in a device for removing phosphates from wastewaters |
-
2013
- 2013-12-12 CN CN201310669563.5A patent/CN103623785B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004058647A1 (en) * | 2002-12-31 | 2004-07-15 | Ku-Su Kim | Wastewater disposal agent and process for preparing thereof |
| CN102500340A (en) * | 2011-09-30 | 2012-06-20 | 北京师范大学 | Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle |
| WO2013102729A1 (en) * | 2012-01-03 | 2013-07-11 | Compagnie Financiere Et De Participations Roullier | Phosphate-based granular material, process for manufacturing same and use thereof in a device for removing phosphates from wastewaters |
| CN102963959A (en) * | 2012-12-03 | 2013-03-13 | 北京师范大学 | Preparation method of microsphere particles capable of slowly releasing nitrogen and adsorbing-biologically degrading petroleum hydrocarbon |
| CN102976499A (en) * | 2012-12-03 | 2013-03-20 | 北京师范大学 | Method for preparing composite board capable of simultaneously slowly releasing nitrogen and adsorbing/biologically degrading petroleum hydrocarbons |
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Granted publication date: 20150909 Termination date: 20161212 |