CN102500340B - Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle - Google Patents
Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle Download PDFInfo
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- CN102500340B CN102500340B CN 201110291350 CN201110291350A CN102500340B CN 102500340 B CN102500340 B CN 102500340B CN 201110291350 CN201110291350 CN 201110291350 CN 201110291350 A CN201110291350 A CN 201110291350A CN 102500340 B CN102500340 B CN 102500340B
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- porous particle
- petroleum hydrocarbon
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- polyoxyethylene
- hydroxybutyrate
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Abstract
The invention discloses a porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and a preparation method of the porous particle. Poly-beta-hydroxybutyrate chloroformic solution with mass fraction of 8% and polyoxyethylene chloroformic solution with mass fraction of 8% are mixed according to the proportion of 4:1; deionized water is dripped into the mixed solution according to the proportion of 1:4, and mixture of the deionized water and the mixed solution is stirred for 4 minutes; obtained mixed liquid is dripped into polyvinyl alcohol solution with mass fraction of 0.3% according to the proportion of 1:1, and mixture is stirred for 4 minutes; polyvinyl alcohol solution with mass fraction of 0.4% is added in the mixture according to the proportion of 2:1, the mixture is centrifugally separated after being stirred for 7 hours to 8 hours, and is washed by deionized water for three times, the porous particle is obtained after the mixture is lyophilized; and the porous particle is taken out to be aired after being cultivated in petroleum hydrocarbon degrading bacterium culture solution for 24 hours. The porous particle and the preparation method thereof are simple in process, a prepared porous particle is high in efficiency to treat pollutants of the petroleum hydrocarbon, and an ecological environment of water cannot be polluted secondarily or damaged.
Description
Technical field
The invention belongs to the physical absorption and the biodegradation processing technology field that are subjected to the oil spill water body, particularly a kind of porous particle that petroleum hydrocarbon is had absorption and biological degradation function and preparation method thereof.
Background technology
In recent years, the demand that oil and goods thereof is day by day increased along with countries in the world, at sea exploit, load and unload, transport and utilize that oil spill accident happens occasionally in the oil process, not only cause a large amount of crude oil to run off, also environment is caused severe contamination, threatening the ecological environment of water body and land-based area with being on the rise, water body environment, natural resources and culture resources etc. are had long-term harm, therefore, the water body oil spill has become the great environmental problem that people must face.
The method of disposing at present the water body oil spill both at home and abroad mainly contains Physical, chemical method and bioanalysis.Chemical method is the technology path of comparative maturity in point-source pollution is administered, and has obtained widely propagation and employment.But adopt chemical method in the water body oil spill is administered, need to add chemical agent in water body, the chemical agent that wherein adds may produce secondary pollution on the one hand, can produce short-term or long-term impact to water ecological setting on the other hand.The characteristics such as bioremediation technology has efficiently, economy, safety, non-secondary pollution, become the on-the-spot important selection approach of removing the water body oil spill, the oil sheet that particularly can't remove mechanical device, when chemical agent is used in restriction again simultaneously, use biological prosthetic its larger superiority of showing.Physisorphtion is that a kind for the treatment of effeciency is high, cost is low, without sediment pollution, simple to operate and can not cause to water ecological setting the method for secondary pollution and destruction, can solve preferably the existing problem of chemical method.But, the cycle of bioremediation technology is long, be subjected to simultaneously whether to exist the restriction of highly effective petroleum alkane degradation bacterium, physisorphtion faces absorption needs problem that adsorbent is remarketed after saturated, then can remedy the deficiency of the two if physisorphtion and biodegradation can be combined, be used for disposing the water body oil spill and will have larger advantage.
But, for the technology that physisorphtion and biodegradation are combined obtains further propagation and employment in the water body oil spill is processed, develop the material that has simultaneously physical absorption and biological degradation function, have great importance for raising water body oil spill treatment effeciency, reduction environmental pollution, but the present technology that also lacks this aspect is reported with research.
Summary of the invention
Purpose of the present invention provides a kind of porous particle that petroleum hydrocarbon is had absorption and biological degradation function and preparation method thereof.Its concrete steps are as follows:
(1) the preparation mass fraction is 8% poly beta-hydroxybutyrate chloroformic solution; The preparation mass fraction is 8% polyoxyethylene chloroformic solution; Be that 4: 1 ratio fully is mixed to get mixed solution with the two in the volume ratio of poly beta-hydroxybutyrate chloroformic solution and polyoxyethylene chloroformic solution;
(2) be that 1: 4 ratio is added drop-wise to deionized water in the step (1) in the mixed solution by volume under the 1000r/min stirring condition, then under the 1000r/min condition, stir 4min;
(3) be by volume 1: 1 ratio the mixed liquor that obtains in the step (2) is added drop-wise to mass fraction under the 400r/min stirring condition be 3% poly-vinyl alcohol solution, then under the 400r/min condition, stir 4min;
(4) be that 2: 1 ratio is that 0.4% poly-vinyl alcohol solution joins in the mixed liquor that obtains in the step (3) with mass fraction by volume, under the 400r/min condition, stir 7～8h, solvent is fully volatilized and cause poly beta-hydroxybutyrate-polyoxyethylene polymerization reaction take place, be to carry out centrifugation under the 6000r/min condition at rotating speed, then with deionized water washing 3 times, freeze drying gets the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene;
(5) the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene in the step (4) is cultivated 24h in the petroleum hydrocarbon degradation bacteria culture fluid, petroleum hydrocarbon degradation bacterium is absorbed and fixed on the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene, and wherein the bacteria containing amount of petroleum hydrocarbon degradation bacteria culture fluid is 5 * 10
10CFU/ml, the consisting of of nutrient solution: NH
4NO
3: 2.0gL
-1, NaCl:10.5g/L, KH
2PO
4: 2.5gL
-1, FeCl
3: 0.10gL
-1, MgSO
4: 3.5gL
-1, CaCl
22H
2O:0.15gL
-1
(6) the compound porous particle of the poly beta-hydroxybutyrate-polyoxyethylene that obtains in the step (5) is taken out air-dry, can obtain petroleum hydrocarbon is had the porous particle of absorption and biological degradation function.
The invention has the beneficial effects as follows that manufacture craft is simple, the porous particle that makes is high to the petroleum hydrocarbon contaminant treatment effeciency, and can not cause secondary pollution and destruction to water ecological setting.
The specific embodiment
The invention provides a kind of porous particle that petroleum hydrocarbon is had absorption and biological degradation function and preparation method thereof.Be that 8% poly beta-hydroxybutyrate chloroformic solution and 40mL polyoxyethylene chloroformic solution fully are mixed to get the 200mL mixed solution with the 160mL mass fraction; The 50mL deionized water is added drop-wise under stirring condition in the above-mentioned 200mL mixed solution obtains the 250mL mixed solution, then under the 1000r/min condition, stir 4min; It is to obtain the 500mL mixed solution in 3% the poly-vinyl alcohol solution that the 250mL mixed liquor that obtains is added drop-wise to the 250mL mass fraction, then stirs 4min under the 400r/min condition; Be that 0.4% poly-vinyl alcohol solution joins in the mixed liquor of above-mentioned 500mL with the 1000mL mass fraction, under the 400r/min condition, stir 7～8h, solvent is fully volatilized and cause poly beta-hydroxybutyrate-polyoxyethylene polymerization reaction take place, then be to carry out centrifugation under the 6000r/min condition at rotating speed, with deionized water washing 3 times, freeze drying gets the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene; Be 5 * 10 with the porous particle that obtains at bacteria containing amount
10Cultivate in the petroleum hydrocarbon degradation bacteria culture fluid of CFU/mL and take out behind the 24h air-dryly, can obtain petroleum hydrocarbon is had the porous particle of absorption and biological degradation function.
The below is that the porous particle that petroleum hydrocarbon is had absorption and a biological degradation function that uses that the inventive method makes has carried out absorption and biodegradation test to the petroleum hydrocarbon in the oil spill water body, further specifies the present invention.
Embodiment
The porous particle that petroleum hydrocarbon is had absorption and a biological degradation function that uses that the inventive method makes has carried out absorption and biodegradation test to the petroleum hydrocarbon in the oil spill water body, the result shows that this porous particle can effectively adsorb and biodegradation the petroleum hydrocarbon in the water body: when the initial concentration of polluted-water Petroleum Hydrocarbon is 66.7mg/L, the concentration of energy water body Petroleum Hydrocarbon is reduced to 0.01mg/L after 12 hours, and porous particle Petroleum Hydrocarbon concentration does not detect after 14 days.
Claims (1)
1. one kind has absorption and the porous particle preparation method of biological degradation function to petroleum hydrocarbon, it is characterized in that the concrete steps of the method are as follows:
(1) the preparation mass fraction is 8% poly beta-hydroxybutyrate chloroformic solution; The preparation mass fraction is 8% polyoxyethylene chloroformic solution; Be that 4: 1 ratio fully is mixed to get mixed solution with the two in the volume ratio of poly beta-hydroxybutyrate chloroformic solution and polyoxyethylene chloroformic solution;
(2) be that 1: 4 ratio is added drop-wise to deionized water in the step (1) in the mixed solution by volume under the 1000r/min stirring condition, then under the 1000r/min condition, stir 4min;
(3) be by volume 1: 1 ratio the mixed liquor that obtains in the step (2) is added drop-wise to mass fraction under the 400r/min stirring condition be 3% poly-vinyl alcohol solution, then under the 400r/min condition, stir 4min;
(4) be that 2: 1 ratio is that 0.4% poly-vinyl alcohol solution joins in the mixed liquor that obtains in the step (3) with mass fraction by volume, under the 400r/min condition, stir 7～8h, solvent is fully volatilized and cause poly beta-hydroxybutyrate-polyoxyethylene polymerization reaction take place, be to carry out centrifugation under the 6000r/min condition at rotating speed, then with deionized water washing 3 times, freeze drying gets the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene;
(5) the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene in the step (4) is cultivated 24h in the petroleum hydrocarbon degradation bacteria culture fluid, petroleum hydrocarbon degradation bacterium is absorbed and fixed on the compound porous particle of poly beta-hydroxybutyrate-polyoxyethylene, and wherein the bacteria containing amount of petroleum hydrocarbon degradation bacteria culture fluid is 5 * 10
10CFU/ml, the consisting of of nutrient solution: NH
4NO
3: 2.0gL
-1, NaCl:10.5g/L, KH
2PO
4: 2.5gL
-1, FeCl
3: 0.10gL
-1, MgSO
4: 3.5gL
-1, CaCl
22H
2O:0.15gL
-1
(6) the compound porous particle of the poly beta-hydroxybutyrate-polyoxyethylene that obtains in the step (5) is taken out air-dry, can obtain petroleum hydrocarbon is had the porous particle of absorption and biological degradation function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110291350 CN102500340B (en) | 2011-09-30 | 2011-09-30 | Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110291350 CN102500340B (en) | 2011-09-30 | 2011-09-30 | Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle |
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| Publication Number | Publication Date |
|---|---|
| CN102500340A CN102500340A (en) | 2012-06-20 |
| CN102500340B true CN102500340B (en) | 2013-05-01 |
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|---|---|---|---|
| CN 201110291350 Expired - Fee Related CN102500340B (en) | 2011-09-30 | 2011-09-30 | Porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and preparation method of porous particle |
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| CN103011380B (en) * | 2012-12-03 | 2013-11-20 | 北京师范大学 | Preparation method of biodegradable oil absorption plate capable of slowly releasing nitrogen element |
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| CN102941071B (en) * | 2012-12-03 | 2014-04-09 | 北京师范大学 | Preparation method of biodegradable oil absorption microsphere with nitrogen nutrient element slow release function |
| CN103663731B (en) * | 2013-12-12 | 2015-04-08 | 北京师范大学 | Porous adsorbent for adsorbing and biologically degrading petrochemical wastewater and preparation method |
| CN103623785B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | Can the suspension oil absorption plate of slowly-releasing phosphorus and biodegradation of petroleum hydrocarbon and preparation method |
| CN103623791B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | Petrochemical wastewater is had to porous adsorption plate material and the preparation method of multiple complex function |
| CN103623781B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | Organic chemical waste water is had to adsorption plate and the preparation method of efficient adsorption function |
| CN103623786B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | What be applied to coal chemical industry enterprises wastewater treatment releases phosphorus adsorption plate and preparation method |
| CN103623792B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | Be applied to multifunctional ceiling attached plate and the preparation method of nitrogen phosphorus disappearance treatment of Organic Wastewater |
| CN103611506B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | May float on biodegradable multifunctional ceiling elaioleucite and the preparation method of the water surface |
| CN103623793B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | Petroleum hydrocarbon contaminated water body is had to porous adsorption plate and the preparation method of several functions |
| CN103623790B (en) * | 2013-12-12 | 2015-09-09 | 北京师范大学 | For polymer-function material and the preparation method of adsorption treatment organic chemical waste water |
| CN103611515B (en) * | 2013-12-12 | 2015-08-05 | 北京师范大学 | For the multi-stage absorption pond of biological reinforced advanced treating coal chemical industrial waste water |
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| US4828695A (en) * | 1987-02-26 | 1989-05-09 | Yamamura Chemical Laboratories, Co., Ltd. | Packaging material for high pressure liquid chromatography and method of making the same |
| CN1695795A (en) * | 2005-04-05 | 2005-11-16 | 天津大学 | Method for preparing ultra macropore type rigid macromolecule medium by using suspension liquid of fine particles of calcium carbonate |
| CN101524637A (en) * | 2009-04-23 | 2009-09-09 | 中国海洋石油总公司 | Preparation method of LSX-molecular-sieve-rich cementing-agent-free absorbent |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002166168A (en) * | 2000-11-30 | 2002-06-11 | Unitika Chem Co Ltd | Method for molding adsorptive porous solid |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4828695A (en) * | 1987-02-26 | 1989-05-09 | Yamamura Chemical Laboratories, Co., Ltd. | Packaging material for high pressure liquid chromatography and method of making the same |
| CN1695795A (en) * | 2005-04-05 | 2005-11-16 | 天津大学 | Method for preparing ultra macropore type rigid macromolecule medium by using suspension liquid of fine particles of calcium carbonate |
| CN101524637A (en) * | 2009-04-23 | 2009-09-09 | 中国海洋石油总公司 | Preparation method of LSX-molecular-sieve-rich cementing-agent-free absorbent |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2002-166168A 2002.06.11 |
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Inventor after: Dou Junfeng Inventor after: Li Shuairan Inventor after: Wang Hongting Inventor after: Cheng Lirong Inventor after: Ding Aizhong Inventor after: Zheng Lei Inventor after: Ma Mengjiao Inventor before: Dou Junfeng Inventor before: Li Shuairan Inventor before: Cheng Lirong Inventor before: Ding Aizhong Inventor before: Zheng Lei Inventor before: Ma Mengjiao Inventor before: Wang Hongting |
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Free format text: CORRECT: INVENTOR; FROM: DOU JUNFENG LI SHUAIRAN CHENG LIRONG DING AIZHONG ZHENG LEI MA MENGJIAO WANG HONGTING TO: DOU JUNFENG LI SHUAIRAN WANG HONGTING CHENG LIRONG DING AIZHONG ZHENG LEI MA MENGJIAO |
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